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Development Of Multiplex Rt-Pcr For Detection And Differentiationof Foot-And-Mouth Disease Virus O And A Serotypes In Turkey, Beyhan Sareyyüpoğlu, İbrahi̇m Burgu

Turkish Journal of Veterinary & Animal Sciences

In this study, a two-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed and evaluated using designed primers for the simultaneous detection and differentiation of Turkish foot-and-mouth disease virus (FMDV) serotypes A and O from clinical samples. In the method, a mix of one universal reverse primer designed from the 2B gene and two serotype-specific forward primers designed from the hypervariable regions of the capsid VP1-1D coding gene of FMDV were used. Totally, 272 FMDV-infected samples collected between 2006 and 2008 constituted the material of the study. mRT-PCR was compared to ELISA in terms of positivity percentage (%) and the …

Our Gift To All Of Us: Ga(Y)Am: Preface, Frank Loesche, Klara ?Ucznik

More Faculty of Arts, Humanities and Business Research

This special issue of AVANT is all about Cognitive Innovation. It is not about CogNovo, the interdisciplinary and international doctoral training programme that produced three different Off the Lip events. It is not about Off the Lip 2017, the novel sympo

Comparative Review Of Education Doctorates In Three Countries.., M East, Eb Hajdukova, Me Carr, Wh Evans, G Hornby

More Faculty of Arts, Humanities and Business Research

No abstract provided.

The Csnp Scanning And Expression Analysis Of The Duck Fth1 Gene, Yu Zhang, Yiyu Tong, Yang Chen, Zhengyang Huang, Zhen Zhu, Yang Zhang, Qi Xu, Guohong Chen

Turkish Journal of Veterinary & Animal Sciences

Ferritin, heavy polypeptide 1 (FTH1) is a kind of ferritin complex that catalyzes the conversion of Fe(II) into Fe(III) to protect the cell from oxidative damage. Recent research has shown that FTH1 is also associated with several disease-related processes, such as inflammation and tumor progression and so on. In this study, the coding sequence (CDS) of FTH1 in Jinding ducks was cloned. The CDS sequence revealed an open reading frame of 546 nucleotides encoding a protein with 181 amino acids. Based on this sequence, the duck FTH1 protein is predicted to have conserved domains typical of eukaryotic ferritin. Only one …

A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi

Turkish Journal of Veterinary & Animal Sciences

Ticks are the main hematophagous arthropods and obligatory ectoparasites that are considered a vector of serious pathogens for animals and humans. The aims of the present survey were to assess the prevalence and diversity of ticks in cattle in Southeast Iran and to determine Coxiella burnetii in the ticks. From May 2014 to April 2015, 583 ticks were collected randomly from 257 cattle. Nested trans-PCR was performed to detect C. burnetii. The overall prevalence of hard tick infestation on cattle was 56.8%. In total, seven different species were identified: Rhipicephalus sanguineus sensu lato (32.6%), Hyalomma anatolicum (28.8%), Hyalomma excavatum (19%), …

Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

The present study was undertaken to investigate the immunohistochemical expression of pulmonary surfactant proteins (SP-A, SP-B, proSP-C) associated with proliferating cell nuclear antigen (PCNA), thyroid transcription factor-I (TTF-I), and lymphocytic phenotypes (CD3+ T and CD79αcy+ B cells) in ovine lungs with exudative and verminous pneumonia. Pneumonic lungs (n = 50) were classified as presenting fibrinous bronchopneumonia (n = 16), abscessing necrotic bronchopneumonia (n = 10), verminous pneumonia (n = 15), and interstitial pneumonia (n = 9). The lungs with fibrinous bronchopneumonia displayed consolidation of lobes with adhesion to surrounding tissues. The lungs with verminous pneumonia were characterized by lungworm sections …

Hematological Effects Of Etomidate And Tricaine In Common Carp, Malgorzata Witeska, Beata Teodorczuk, Katarzyna Lugowska

Turkish Journal of Veterinary & Animal Sciences

Hematological effects of etomidate and tricaine at concentrations causing general anesthesia or sedation of carp juveniles were compared. Recovery of body balance took similar time in the case of both anesthetics, but fish treated with etomidate were depressed for several hours, while those subjected to tricaine immediately regained normal behavior. Immediately after exposure anesthetics caused minor hematological changes; the only statistically significant effects (decrease of hematocrit and plasma glucose) occurred after sedation with etomidate. One week after exposure a significant increase in mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration occurred in all groups of fish compared to the previous …

Influence Of Dietary Vitamin D3 Supplementation On The Color Changes In Longissimus Lumborum, Vastus Lateralis, Semitendinosus, And Semimembranosus Beef Muscles, Urszula Karolina Rafalska

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effect of dietary vitamin D3 supplementation on changes in color and heme pigment (myoglobin and metmyoglobin) in 4 beef muscles. The muscles came from 48 crossbred bulls that were subjected to supplementation with vitamin D3 at 4 different doses: 0.0 (control group), 3.5, 7.0, and 10.0 x 106 IU. The 3.5 x 106 IU dose was administered daily for 6 days, before the morning feeding. Four days before slaughter, the supplementation was withdrawn. The dose of 7.0 and 10.0 x 106 IU was administered for 3 days and withdrawn 7 days …

Radiographic Examination Of Hip Joint Geometry In Healthy Sivas Kangal Dogs, Murat Sarierler, Zeynep Bozkan Tatli, İbrahi̇m Akin, Rahi̇me Yaygingül, Onur Özgün Deri̇ncegöz

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the normal ranges of proximal femoral and acetabular parameters in 26 adult healthy Sivas Kangal dogs (SKDs). Significant differences were observed between the sexes in hip axis length (HAL) of 8.03 ± 0.82 cm and 7.47 ± 0.40 cm, femoral neck axis length-a (FNALa) of 6.40 ± 0.55 cm and 6.09 ± 0.27 cm, FNALb of 5.09 ± 0.47 cm and 4.78 ± 0.31 cm, acetabular width (AW) of 1.62 ± 0.35 cm and 1.37 ± 0.24 cm, femoral shaft diameter (FSD) of 3.11 ± 0.31 cm and 2.88 ± 0.21 cm, …

Presidential Politics And Judicial Review, Robert L. Glicksman, David Aldeman

GW Law Faculty Publications & Other Works

This Article assesses the impact of judicial review on one of the nation’s foundational environmental statutes, the National Environmental Policy Act (NEPA). Based on litigation spanning fifteen years, we find that the stringency of judicial review is driven by the interaction of judicial ideology and presidential politics. Our principal findings are two-fold: First, judicial ideology, here defined by political party affiliation, is most influential when NEPA’s environmental goals conflict with the politics of the presidential administration in power. Second, the influence of judicial ideology is mediated by the distribution of cases across federal circuits and the ideological balance of judges …

How Adverse Outcome Pathways Can Aid The Development And Use Of Computational Prediction Models For Regulatory Toxicology, Clemens Wittwehr, Hristo Aladjov, Gerald Ankley, Hugh Byrne, Joop Deknecht

Articles

Efforts are underway to transform regulatory toxicology and chemical safety assessment from a largely empirical science based on direct observation of apical toxicity outcomes in whole organism toxicity tests to a predictive one in which outcomes and risk are inferred from accumulated mechanistic understanding. The adverse outcome pathway (AOP) framework provides a systematic approach for organizing knowledge that may support such inference. Likewise, computational models of biological systems at various scales provide another means and platform to integrate current biological understanding to facilitate inference and extrapolation. We argue that the systematic organization of knowledge into AOP frameworks can inform and …

Synthesis And Characterization Of A Multifunctional Gold-Doxorubicin Nanoparticle System For Ph Triggered Intracellular Anti-Cancer Drug Release, Ghanesh Khutale, Alan Casey

Articles

A nanoparticle drug carrier system has been developed to alter the cellular uptake and chemotherapeutic performance of an available chemotherapeutic drug. The system comprises of a multifunctional gold nanoparticle based drug delivery system (Au-PEG-PAMAM-DOX) as a novel platform for intracellular delivery of doxorubicin (DOX). Spherical gold nanoparticles were synthesized by a gold chloride reduction, stabilized with thiolated polyethylene glycol (PEG) and then covalently coupled with a polyamidoamine (PAMAM) G4 dendrimer. Further, conjugation of an anti-cancer drug doxorubicin to the dendrimer via amide bond resulted in Au-PEG-PAMAM-DOX drug delivery system. Acellular drug release studies proved that DOX released from Au-PEG-PAMAM-DOX at …

In Vitro Comparative Cytotoxicity Study Of Aminated Polystyrene, Zinc Oxide And Silver Nanoparticles On A Cervical Cancer Cell Line, Akash Sharma, Brian Gorey, Alan Casey

Articles

Nanoparticles use in nano-biotechnology applications have increased significantly with Aminated polystyrene amine (AmPs NP), Zinc oxide (ZnO NP), and Silver (Ag NP) nanoparticles utilized in wide variety of consumer products. This has presented a number of concerns due to their increased exposure risks and associated toxicity on living systems. Changes in the structural and physicochemical properties of nanoparticles can lead to changes in biological activities. This study investigates, compares, and contrasts the potential toxicity of AmPs, ZnO and Ag NPs on an in vitro model (HeLa cells) and assesses the associated mechanism for their corresponding cytotoxicity relative to the surface …

Carbon Nanomaterials And Their Application To Electrochemical Sensors: A Review, Aoife Power, Shaneel Chandra, Brian Gorey, James Chapman

Articles

Carbon has long been applied as an electrochemical sensing interface owing to its unique electrochemical properties. Moreover, recent advances in material design and synthesis, particularly nanomaterials, has produced robust electrochemical sensing systems that display superior analytical performance. Carbon nanotubes (CNTs) are one of the most extensively studied nanostructures because of their unique properties. In terms of electroanalysis, the ability of CNTs to augment the electrochemical reactivity of important biomolecules and promote electron transfer reactions of proteins is of particular interest. The remarkable sensitivity of CNTs to changes in surface conductivity due to the presence of adsorbates permits their application as …

Biofunctionalized Capillary Flow Channel Platform Integrated With 3d Nanostructured Matrix To Capture Circulating Tumor Cells, Shashwat Banerjee, Ganesh Khutale, Vrushali Khobragade, Narendra Kale, Milind Pore, Govind Chate, Archana Jalota-Badhwar, Manoj Dongare, Jayant Khandare

Articles

Circulating tumor cells (CTCs) from peripheral blood account genetic information for cancer diagnosis and overall disease monitoring. Analysis of “liquid biopsy” holds immense promise as it may lead to new approaches for cancer treatment. The study reports effective and continuous flow microchannel system for isolating CTCs using transferrin conjugated 3D matrix synthesized by crosslinking polyethylene glycol-Fe3O4 nanostructures for rapid and efficient capturing of CTCs. The platform provides option of using multiple microchannel units in series that can influence higher cell-capture efficiency due to increasing cell-substrate contact frequency. CTCs are captured with high efficiency even at low concentration of target cells …

Integration Of Biological Effects, Fish Histopathology And Contaminant Measurements For The Assessment Of Fish Health: A Pilot Application In Irish Marine Waters, Michelle Giltrap, Jenny Ronan, John Bignell, Brett Lyons, Evelyn Collins, Heather Rochford, Brendan Mchugh, Evin Mcgovern, Lorraine Bull, James Wilson

Articles

This study investigates the use of a weight of evidence (WOE) approach to evaluate fish health status and biological effects (BEs) of contaminants for assessment of ecosystem health and discusses its potential application in support of the Marine Strategy Framework Directive (MSFD). External fish disease, liver histopathology and several BEs of contaminant exposure including 7-ethoxy resorufin O-de-ethylase (EROD), acetylcholinesterase (AChE), bile metabolites, vitellogenin (VTG) and alkali labile phosphates (ALP) were measured in two flatfish species from four locations in Ireland. Contaminant levels in fish were generally low with PCBs in fish liver below OSPAR environmental assessment criteria (EAC). There were …

Finding A Fit For Nonprofit Hospitals: A National Perspective Of State Property Tax Exemption Laws, Jillian A. Swogier

Seton Hall Journal of Legislation and Public Policy

No abstract provided.

Sexy, Thin, And White: The Intersection Of Sexualization, Body Type, And Race On Stereotypes About Women And Women's Body Dissatisfaction, Ellen A. Stone

Theses and Dissertations--Psychology

The vast majority of media images present one idealized type of woman: she is thin, sexualized, and White. While research has shown that there are stereotypes associated with sexualized women, research has not addressed whether these stereotypes vary based on other characteristics such as body type and race. The current study aimed to examine the stereotypes associated with women who varied in body size, sexualization, and race. Additionally, the current study examined whether exposure to differing portrayals of women was related to endorsement of gender stereotypes and body dissatisfaction. College-aged students (n = 226, 161 women) rated four traits …

Testing A Values-Based Approach To Healthcare Decision-Making In Older Adults, Paul Jefferson Geiger

Theses and Dissertations--Psychology

Despite natural declines in physical and cognitive function, older adults maintain good emotion regulation abilities, leading to emotional wellbeing and resilience. This phenomenon can partially be explained by socioemotional selectivity theory (SST), which posits that when time is perceived as a limited resource, older adults focus attention on positive environmental stimuli to regulate emotions. Although this positivity effect maintains emotional wellbeing, it may disrupt information processing related to healthcare decision-making. Older adults request less information from their doctors, are less likely to ask for a second opinion, make their decisions more quickly, and devote more attention to positive medical information, …

Behavioral Deficits Across Development In A Novel Mouse Model Of Fetal Ethanol Effects, Andrew B. Hawkey

Theses and Dissertations--Psychology

Fetal Alcohol Spectrum Disorders (FASD) are a spectrum of anatomical, developmental and neurobehavioral impairments resulting from ethanol (ETOH) exposure during fetal development. Efforts to develop and screen novel pharmacotherapies against fetal ETOH effects depend heavily upon rodent models to provide indicators of the safety and efficacy of such compounds, in addition to helping better understand the underlying mechanisms to develop and test these pharmacotherapies. The following experiments describe the development of a novel mouse model of FASD using behavioral batteries to assess behavioral or cognitive deficits in juvenile and adolescent offspring (Experiment 1, Experiment 2) and whether deficits with this …

Too Pretty For Homework: The Academic Correlates Of Sexualized Gender Stereotypes Among Adolescent Girls, Andrew A. Nelson

Theses and Dissertations--Psychology

Girls grow up in a culture of ubiquitous female sexualization, and this culture propagates stereotypes that could interfere with their academic outcomes. The current study examined the academic correlates of these sexualized gender stereotypes (SGS) among early adolescent girls. Girls (N = 99) aged 11 to 14 (M_age = 12.4 years, SD = .57 years) completed a survey assessing their academic performance, attitudes, and beliefs. The survey also assessed the degree to which girls believed that boys and girls should act in accordance with these sexualized gender stereotypes. Results indicated that higher endorsement of sexualized gender stereotypes …

Dismantling The Five Factor Form, Stephanie L. Rojas

Theses and Dissertations--Psychology

The purpose of this dissertation was to provide a further validation of the Five Factor Form (FFF; Rojas & Widiger, 2014). The FFF is a brief (one page) rating form that assesses for adaptive and maladaptive variants of both poles for each of six facets for the five domains of the five-factor model. Two prior validation studies of the FFF have been completed using the items as they are scored within the FFF (Rojas & Widiger, 2014, in press). However, the FFF has a unique scoring system in which each item has normal and abnormal variants at both poles (e.g., …

Internalizing And Externalizing Dysfunction: An Integrative Model Of Adolescent Drinking, Leila Guller

Theses and Dissertations--Psychology

Separate externalizing and internalizing pathways to problem drinking have been described. However, internalizing and externalizing are substantially correlated, thus, there is good reason to believe that these two forms of dysfunction behaviors do not operate independently.

We tested an integrative developmental model of transactions among internalizing symptomatology, externalizing personality, and psychosocial learning in the prediction of both drinking problems and future internalizing symptoms. To do so, we studied a large sample (n = 1910, 49.9% female) of children over a critical developmental period, from the spring of 5th (last year elementary school) grade through the spring of 9th grade (first …

Maintenance Of Positive Affect Following Pain In Younger And Older Adults, Ian Andres Boggero

Theses and Dissertations--Psychology

Socioemotional selectivity theory posits that as people age, they become motivated and successful at maximizing positive emotions and minimizing negative ones. Yet, 70% of older adults report physical pain, which is associated with negative affect. The strategies and resources that older adults use to maintain positive affect in the face of pain remain largely unknown. Specific positivity-enhancing strategies include recalling, recognizing, and responding to positive stimuli and prioritizing close over knowledgeable social partners. Executive functions (EF, i.e., task-switching, working memory, and inhibition) and heart rate variability (HRV) may be important resources for coping with pain. The current project used two …

The Influence Of Cocaine-Related Images On Inhibitory Control In Cocaine Users, Erika Pike

Theses and Dissertations--Psychology

Cocaine users display impaired inhibitory control. The influence of cocaine-related stimuli on inhibitory control has not been assessed. The Attentional Bias-Behavioral Activation (ABBA) task uses cocaine and neutral images as cues to determine if drug-related images impair inhibitory control in cocaine users. This dissertation was designed to assess the influence of cocaine images on inhibitory control in cocaine users through the conduct of studies designed to address four aims. The first aim was to demonstrate that cocaine users display impaired inhibitory control following cocaine images compared to neutral images on the ABBA task. This was accomplished through the conduct of …

Optimism And Pain Interference In Aging Women, Stephanie T. Judge

Theses and Dissertations--Psychology

Pain interferes with people's daily lives and often limits the extent to which they can pursue goals and engages in activities that promote well-being. The present study test how optimism affects and is affected by pain interference and activity among older women. Every three months for two years, middle- and older-age women (N = 199) complete daily diaries at home for a seven-day period, reporting their daily pain, pain interference, and activity. Optimism was measured at baseline and end-of-study. Multilevel models test the between- and within-person relationships among pain, optimism, pain interference and activity. Pain best predicted pain interference …

Risk Factors For Abuse Of Prescription Stimulants In College Students: A Dissertation, Eric A. Haak

Theses and Dissertations--Psychology

The abuse of prescription stimulants among college students is a growing public health concern. While these substances are often viewed as safe, “smart drugs”, they are associated with a number of maladaptive outcomes, ranging from poorer academic performance to cardiovascular incidents and even death. To date, the majority of research on the abuse of these substances have focused on demographic factors which are not amenable to intervention, such as race, Greek organization status, and class rank. The current study examined family factors, academic stress, and impulsivity as potential risk factors which are amenable to intervention. In a sample of 335 …

Caars-S:L Infrequency Index Validation: A Pilot Comparison Of Paper And Online Assessments, Elizabeth R. Wallace

Theses and Dissertations--Psychology

One obstacle to the accurate diagnosis of ADHD in college students is malingering, although many symptom self-report measures do not contain feigning validity scales. The Infrequency Index (CII) for the Conners’ Adult ADHD Rating Scale–Self-Report: Long Version (CAARS-S:L) was developed for this purpose, although further validation of the index is needed. Another topic of interest in ADHD malingering research is the increasing use of online assessments. Little is known about how ADHD is malingered in an online format, particularly on the CAARS-S:L. The current study aims to integrate these strands of research by examining the utility of the CII in …

From A Tweet To The Street: The Effect Of Social Media On Social Movement Theory, Elizabeth Romary

Honors Theses

This thesis studies the role of social media within social movements and social movement theory and focuses on applying these theories to the Ni Una Menos movement of Argentina. I focus on three social movement theories: the resource mobilization theory, the political process theory, and the cultural approach. I also analyze the rise of social media in the 21st century and how they fit within the framework of the three theories. I then apply these theories to the Ni Una Menos movement. I argue that the Ni Una Menos movement emerged as a cultural movement, and shifted towards a resource …

Altruism, Autonomy, And Human Dignity For The Sustainability Of Post-Mortal Organ Donation, Aiyub Mohammad Fahad Alwehaibi

Electronic Theses and Dissertations

In most countries, the shortage for transplant organ sources has become common due to the significant increase in number of awaiting organ recipients, which, in turn, urges countries develop systemic programs in order to increase the number of organ donors and resolve their problems. Generally, either living or deceased organs are accepted in transplant practice, yet, each source holds its own benefits and adverse effects. Living organ donation encounters controversial and problematic issues at individual, social, and humanity levels. Cadaveric organ donation faces the obstacles of the criteria of death, which vary from cultural to medical perspectives, and the effective …