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2017

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Cadaveric Evaluation Of The Location Of Arteries And Their Anastomotic Territories On The Face: The Feasibility Determination Of Risky Severe Complications Regarding To The Filler Injections Sites, Benrita Jitaree Jan 2017

Cadaveric Evaluation Of The Location Of Arteries And Their Anastomotic Territories On The Face: The Feasibility Determination Of Risky Severe Complications Regarding To The Filler Injections Sites, Benrita Jitaree

Chulalongkorn University Theses and Dissertations (Chula ETD)

The facial aging features are most concerns; therefore, the filler injection procedures are selected to provide the youthful face. However, the several complications following arterial devastating have been reported continually. Therefore, this study objectively evaluated the arterial location relative to filler injection sites. Furthermore, the arterial anastomoses and arterial supply of each injection site were also studied to verify the filler embolic pathways. This dissection was performed on 62 hemi-faces Thai embalmed cadavers to investigate nearest artery and its location of all injection sites. While, the 20 hemi-faces Thai embalmed cadavers were employed with Sihler's staining procedure to examine the …

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Molecular Epidemiology And Genome Analysis Of Hepatitis E Virus In Swine And Blood Donors, Duangnapa Intharasongkroh Jan 2017

Molecular Epidemiology And Genome Analysis Of Hepatitis E Virus In Swine And Blood Donors, Duangnapa Intharasongkroh

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hepatitis E virus (HEV) infection affects approximately 20 million individuals and results in more than 50,000 deaths each year. The highest prevalence is in Asia where contaminated food and water are often the sources of infection. There was the difference among the genotype of human Hepatitis E virus which genotype 1 to 4 can infect human. HEV genotypes 1 and 2 are found only in humans and are responsible for viral hepatitis outbreaks, while genotypes 3 and 4 are zoonotic. The HEV infection in Thailand caused by HEV genotype 3f and usually found among adult males. However, the route of …

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Vitamin D Receptors Distribution And Mapk Activation In Cigarette Smoke Induced Emphysema Rats, Fatist Okrit Jan 2017

Vitamin D Receptors Distribution And Mapk Activation In Cigarette Smoke Induced Emphysema Rats, Fatist Okrit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Rat model was used in this study in order to investigate subcellular vitamin D receptors (VDR) distribution and mitogen activated protein kinase (MAPK) alteration in the lungs of emphysema development after cigarette smoke exposure for 7 days (acute) and 14 days (subacute). Thirty male Wistar rats were randomly divided into 3 groups per each time point; 1) control (air exposed), 2) no-filter (smoke exposed without cigarette filter) and 3) filter (smoke exposed with cigarette filter) (n=5 per group). Each rat was daily and constantly exposed to 6 cigarettes smoke for 7 or 14 days. After 24 hours of the last …

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Molecular Epidemiology Of Human Enterovirus In Various Clinical Specimens With Suspected Enterovirus Infection, Jira Chansaenroj Jan 2017

Molecular Epidemiology Of Human Enterovirus In Various Clinical Specimens With Suspected Enterovirus Infection, Jira Chansaenroj

Chulalongkorn University Theses and Dissertations (Chula ETD)

Many infectious diseases have been well recognized caused by several genotypes of human enterovirus species which significantly in public health. Publications worldwide have reported on the emerging and re-emerging of enteroviruses. Until now, epidemiological surveillance data, clinical complication and evolutionary history regarding these viruses in actual on the Asia continent are still limited. To address these concerns, this study aimed to establish comprehensive population-based surveillances, and provided evidence for the evolution in many diseases such as acute haemorrhagic conjunctivitis, herpangina, hand foot mouth disease, acute gastroenteritis, poliovirus and meningitis caused by enteroviruses. Employing reverse transcription-polymerase chain reaction and sequencing approaches …

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Generation And Characterization Of Hla-Universal, Ipsc-Derived Platelets, Phatchara Norbnop Jan 2017

Generation And Characterization Of Hla-Universal, Ipsc-Derived Platelets, Phatchara Norbnop

Chulalongkorn University Theses and Dissertations (Chula ETD)

Platelet demand has been increased around the world resulting from many factors including the rise of aging population, hematological malignancy incidence, and hematopoietic stem cell transplantation. In addition, inadequacy of donors or matched donors, time consuming, cost, the risk of transfusion-transmitted infections and transfusion-associated reactions, and platelet transfusion refractoriness are limits of this approach. To solve these problems, here we propose to generate platelets in vitro which do not induce alloimmunity to HLA class I, a major cause of immune factors in platelet transfusion refractoriness. In this study, we have knocked out β2-microglobulin gene (β2m) in iPSCs using paired CRISPR/Cas9 …

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Assessment Of Human Papillomavirus Detection Using Different Methods And Clinical Samples, Pornjarim Nilyanimit Jan 2017

Assessment Of Human Papillomavirus Detection Using Different Methods And Clinical Samples, Pornjarim Nilyanimit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Human papillomavirus (HPV) is the etiological agent implicated cancers include those of the cervix, vulva, vagina, penis, anus, rectum and oropharynx. Currently, the Papanicolaou (Pap) smear is the recommended for cervical cancer screening but only a small percentage of women follow this screening. Overall, this thesis aimed to develop the technique and type of specimen to detect HPV DNA in Thai population and to characterize of the HPV genotypes in Thai patients with anogenital warts. Cervical swab and urine sample of 116 women and 100 men were collected before the Pap smear test. The histological examination of women can classified …

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Lipoic Acid-Incorporated Calcium Carbonate Nanoparticlesfor Controlling Of Adipose Derived Stem Cells (Adscs) Differentiation And Adipocyte Function, Sarocha Cherdchom Jan 2017

Lipoic Acid-Incorporated Calcium Carbonate Nanoparticlesfor Controlling Of Adipose Derived Stem Cells (Adscs) Differentiation And Adipocyte Function, Sarocha Cherdchom

Chulalongkorn University Theses and Dissertations (Chula ETD)

CaCO3 NPs has received a growing interest due to its potential properties as a safe drug carrier. The LA-CaCO3 NPs were synthesized by a chemical precipitation method and characterized using transmission electron microscopy, thermogravimetric analysis, and zeta potential measurement. We used LA-CaCO3 NPs to evaluate their therapeutic effects in lipid accumulation, lipolysis, adipogenesis, and adipokines expression in ADSCs and differentiated adipocytes. The average particle sizes were observed to be 10-20 nanometers. Thermogravimetric analysis results confirmed the percentages of LA in CaCO3 NPs. The zeta potential showed a negative charge -30.9 mV. Our findings indicated that LA-CaCO3 NPs were apparently more …

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Effects Of Genistein On Non-Alcoholic Steatohepatitis (Nash) Induced By High-Fat High-Fructose Diet In Bilateral Ovariectomized Rats, Sudaporn Pummoung Jan 2017

Effects Of Genistein On Non-Alcoholic Steatohepatitis (Nash) Induced By High-Fat High-Fructose Diet In Bilateral Ovariectomized Rats, Sudaporn Pummoung

Chulalongkorn University Theses and Dissertations (Chula ETD)

To investigate the effects of genistein on estrogen deficiency with high-fat high-fructose (HFHF) diet-induced NASH rats. Female Sprague-Dawley rats (n=48) were randomly divided into ovariectomized (OVX) and non-ovariectomized (non-OVX) group. Both rat groups, further allocated into 3 subgroups; controls, rats fed with HFHF diet (NASH), and rats fed with HFHF diet plus daily 16 mg/kg BW of genistein (NASH+Gen) for 8 weeks. Serum samples were collected for liver enzymes (AST and ALT) and TNF-α level analysis. Liver tissue samples were harvested for histopathology, hepatic lipid accumulation (oil red o stained, FFA and TG level), hepatic lipid peroxidation (MDA level), hepatic …

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Evaluation Of Lipid Accumulation And Lipolysis Activity In Human Subcutaneous Adipocytes And Human Visceral Adipocytes By Α-Lipoic Acid, Α-Lipoic Acid-Incorporated Caco3 Nanoparticles And Caffeine., Sunisa Kaewdang Jan 2017

Evaluation Of Lipid Accumulation And Lipolysis Activity In Human Subcutaneous Adipocytes And Human Visceral Adipocytes By Α-Lipoic Acid, Α-Lipoic Acid-Incorporated Caco3 Nanoparticles And Caffeine., Sunisa Kaewdang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Recently, much attention has been paid to natural plant-derived compounds as an alternative strategy for developing anti-obesity agents with minimal detrimental effects. Among various natural compounds, caffeine (1, 3, 7-trimethyl xanthine) and alpha lipoic acid (1, 2-dithiolane-3-pentanoic acid; LA) are the natural agents which show a promising result in decreasing fat deposit. Moreover, we have improved efficiencies of LA by CaCO3 NPs because LA had low bioavailability. In the present study, we investigated the effect of combination between caffeine and LA-CaCO3 NPs in lipid accumulation reduction and lipolysis stimulation in human subcutaneous adipocytes and human visceral adipocytes. Our results showed …

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Effects Of Curcumin On Oxidative Stress, Inflammation And Apoptosis In L-Arginine Induced Acute Pancreatitis In Mice, Thidarat Chingchit Jan 2017

Effects Of Curcumin On Oxidative Stress, Inflammation And Apoptosis In L-Arginine Induced Acute Pancreatitis In Mice, Thidarat Chingchit

Chulalongkorn University Theses and Dissertations (Chula ETD)

L-arginine (L-arg) is an amino acid that has been used to induce acute pancreatitis (AP) in mice. Curcumin is an active ingredient constituent of rhizomes of the plant Curcuma longa Linn. It has been used for the treatment of inflammatory conditions and shown to exhibit a variety of biological activities such as anti-inflammatory and anti-oxidant. The present study aims to examine the effects of curcumin on oxidative stress, inflammation and apoptosis in AP. Male ICR mice were randomly divided into 4 groups. 1) Control group, mice will be received i.p. of 1% DMSO as a vehicle. 2) AP group, mice …

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Effect Of Calcium Citrate In Calcium Carbonate Nanoparticles On Hk-2 Cells In Acidic Environment, Tipagorn Udomsinsirikul Jan 2017

Effect Of Calcium Citrate In Calcium Carbonate Nanoparticles On Hk-2 Cells In Acidic Environment, Tipagorn Udomsinsirikul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Metabolic acidosis exerts several cellular and tissue dysfunctions, such as increases mineral bone and protein degradation, induces inflammation, oxidative stress and cell death. These complications lead to multiple organ failure, including chronic kidney disease. Sodium bicarbonate and Shohl's solution are commonly used to treat metabolic acidosis. However, these agents cause frequent gastrointestinal adverse effects. Hence, we invented the new calcium carbonate nanoparticles to use as a drug delivery system for carrying citrate to renal cell which we hypothesize to reduce adverse effects of metabolic acidosis. We studied the physical characteristics and pharmacological effects of calcium citrate nanoparticle (Cacit) and calcium …

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Carotenoids And Metabolism Of The Green Microalga Chlorococcum Sp. 8367re Under Stress Conditions, Kantima Janchot Jan 2017

Carotenoids And Metabolism Of The Green Microalga Chlorococcum Sp. 8367re Under Stress Conditions, Kantima Janchot

Chulalongkorn University Theses and Dissertations (Chula ETD)

Green microalgae are one of the natural resources for productions of various and beneficial bioactive compounds, such as UV-screening compounds and carotenoids. This study focuses on the changes of carotenoids and metabolisms in the green microalga Chlorococcum sp. 8367RE under stress conditions. The results showed that stresses by salt, light, drought, and nitrogen starvation affected on alterations of morphological characteristics. Interestingly, size of cells culturing under salt stresses dramatically increased. It should be noted that KCl stress caused the enlargement of cell sizes approximately 5 folds. Carotenoids were identified and quantified by using ultra-high performance liquid chromatography (UHPLC) and mass …

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Functional Characterization Of Natural Sunscreen Compound Mycosporine-2-Glycine From Extremophilic Cyanobacterium Halothece Sp. Pcc 7418 In Macrophage Cell Line And Fresh Water Cyanobacterium, Supamate Tarasuntisuk Jan 2017

Functional Characterization Of Natural Sunscreen Compound Mycosporine-2-Glycine From Extremophilic Cyanobacterium Halothece Sp. Pcc 7418 In Macrophage Cell Line And Fresh Water Cyanobacterium, Supamate Tarasuntisuk

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mycosporine-2-glycine (M2G) is a rare UV-screening compound in a group of mycosporine-like amino acids (MAAs). This molecule is found in only two cyanobacteria; Euhalothece sp. LK-1 and Aphanothece halophytica (Halothece sp. PCC 7418). The structure of M2G composed of 4-deoxygadusol as a core structure, attached by 2 molecules of glycine at C1 and C3 positions. M2G has a maximal adsorption at 331 nm and a better antioxidative activity than other MAAs. Thus, it is interesting in determination of other activities for further cosmeceutical and pharmaceutical applications. In this study, a high purity M2G was successfully extracted and purified from Halothece …

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Theoretical Studies On Electronic Properties Of Curved Graphene Quantum Dots And Lithium Adsorption On Graphene Quantum Dots, Naruwan Pattarapongdilok Jan 2017

Theoretical Studies On Electronic Properties Of Curved Graphene Quantum Dots And Lithium Adsorption On Graphene Quantum Dots, Naruwan Pattarapongdilok

Chulalongkorn University Theses and Dissertations (Chula ETD)

Graphene quantum dots (GQDs), with their attractive properties, are of interest to apply for electronic devices. Therefore, this dissertation aimed to investigate the electronic of curved graphene quantum dots (CGQDs) and the adsorption of lithium on GQDs. For the first part, two shapes of flat GQDs, rhomboidal (RGQDs) and hexagonal (HGQDs), were modified to make CGQDs with different folding axes and angles. Stabilities and electronic properties of CGQDs were studied using PBE/DNP. The results showed that the deformation energies of GQDs depend on sizes and folding axes but not their shapes. HOMO-LUMO gap variations, both widening and narrowing the gap, …

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Development Of Data Acquisition Technique For Computed Tomography Of Simplified Distillation Column Model As Used In Petroleum And Petrochemical Plants, Dhanaj Saengchantr Jan 2017

Development Of Data Acquisition Technique For Computed Tomography Of Simplified Distillation Column Model As Used In Petroleum And Petrochemical Plants, Dhanaj Saengchantr

Chulalongkorn University Theses and Dissertations (Chula ETD)

This dissertation propose the development of data acquisition technique for computed tomography of simplified distillation column model as used in petroleum and petrochemical industries. The aim of this dissertation is to apply the computed tomography technique to inspect the internal structure of distillation column regardless of complex system to move radiation detectors and source. The developed data acquisition technique allows the operator to move radiation detectors and radiation source by follow designed algorithm. The scanning data are reconstructed using filtered back projection algorithm to evaluate internal structure at the region of interest from cross sectional reconstructed image. In this dissertation, …

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Development Of Technique For Screening Liquids In Unopened Bottle Using Low Energy X-Ray Transmission, Parkphum Orachorn Jan 2017

Development Of Technique For Screening Liquids In Unopened Bottle Using Low Energy X-Ray Transmission, Parkphum Orachorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Since August 2006 after the discovery of terrorists' attempts to explode airplanes with liquid explosives in United Kingdom, each airline passenger is allowed to carry only a maximum of 100 ml liquid in each container and a total of not more than 1,000 ml in carry-on luggage. This measure was adopted to prevent bringing sufficient amount of fuel oil, alcohol, hydrogen peroxide or other flammable and explosive liquids into the aircraft. In this research, X-ray transmission technique was experimentally investigated using low energy X-rays between 13.6 - 43.5 keV emitted from 238Pu radioisotopic source and linear attenuation coefficient of liquids …

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Factors Relating To Methamphetamine Relapse Risk Among Clients In The Substance Rehabilitation Center Of National Narcotics Boards In West Java, Indonesia, Gian Nurmaindah Hendianti Jan 2017

Factors Relating To Methamphetamine Relapse Risk Among Clients In The Substance Rehabilitation Center Of National Narcotics Boards In West Java, Indonesia, Gian Nurmaindah Hendianti

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purposes of this descriptive correlational research were to describe methamphetamine relapse risk and examine the relationship between self-efficacy, outcome expectancy, motivation, coping, emotional state, craving, social support, and methamphetamine relapse risk among methamphetamine users. The purposive sample of 165 who met the inclusion criteria were recruited from clients admitted to a primary phase treatment in the Substance Rehabilitation Center of National Narcotics Board in West Java, Indonesia. The research instruments were: 1) the demographic characteristic questionnaire, 2) Drug Taking Confidence Questionnaire, 3) Stimulant Effect Expectancy Questionnaire, 4) Stage of Change Readiness and Treatment Eagerness Scale version 8.0 for Drug, …

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A Development Of Thai Moral Integrity Scale For Professional Nurses, Jinda Nunthawong Jan 2017

A Development Of Thai Moral Integrity Scale For Professional Nurses, Jinda Nunthawong

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this study was to develop the Thai Moral Integrity Scale (TMIS), and to test its psychometric properties. The construct definition and content domains of the TMIS were developed through the intensive literature reviews and test the content validity by experts. This used to guide the conceptual framework in this instrument. The content validity was examined using the panel of five experts. The content validity index (S-CVI) was 1.00. The Moral Integrity Scale was consists 27 items with five Likert scale. The result of psychometric properties from 502 professional nurses by using exploratory factor analysis indicated that there …

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Predicting Factors Of Recovery Among Persons With Major Depressive Disorder, Nusra Vorapatratorn Jan 2017

Predicting Factors Of Recovery Among Persons With Major Depressive Disorder, Nusra Vorapatratorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purposes of this descriptive research was to examine the causal relationship between strength self-efficacy, resourcefulness, purpose in life, social support, alcohol abuse, and recovery among persons with major depressive disorder (MDD). The conceptual framework was guided by Schlotfeldt's Health Seeking Model. 444 participants with MDD. who attended outpatient department, mental health and psychiatric clinics, and mental health and psychiatric division in eight hospitals from all part of Thailand participated in this study. The participants were obtained by simple random sampling. The research instruments included a personal data sheet, the Strength Self-Efficacy Scale, the Resourcefulness Scale, the Purpose in Life …

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Self-Care Of Older Persons Living With Diabetes In Vietnam: A Qualitative Study, Quyen Truong Thi Mai Jan 2017

Self-Care Of Older Persons Living With Diabetes In Vietnam: A Qualitative Study, Quyen Truong Thi Mai

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to describe the meaning of "self-care" from the perspectives of older persons living with diabetes and explored the self-care behaviors of older persons living with diabetes. The qualitative method was applied in this study. Data were collected by in-depth interview of 16 older persons with Type 2 diabetes in Vietnam. In addition, field note and observation note were written on the reflections, ideas, and meaning of self-care and self-care practices during the data collection process. All data were analyzed by using content analysis method. The results of the study were reflected the self-care from the perspective of …

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Prevalence Of Chronic Temporomandibular Disorder Pain And Comorbidities In A Group Of Thai Patient: A Cross Sectional Study, Tra Thu Nguyen Jan 2017

Prevalence Of Chronic Temporomandibular Disorder Pain And Comorbidities In A Group Of Thai Patient: A Cross Sectional Study, Tra Thu Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: 1. to investigate the prevalence of chronic temporomandibular disorder (TMD) and its comorbidities in a group of Thai patients; 2. to evaluate the relationship between pain parameters (pain duration, pain intensity, and pain frequency) with the number of comorbidities and widespread pain index (WPI); 3. to assess the relationship between the presence of chronic pain with familial history in TMD pain patients. Methods: A cross-sectional study was undertaken of 351 outpatients. Patients were given an interviewer-administered questionnaire which contained four parts (demographic data, pain parameters, WPI and comorbidities diagnosis). Diagnosis was made in accordance with Diagnostic Criteria for Temporomandibular …

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The Effect Of Uncertainty Management Program On Quality Of Life Among Vietnamese Women With Post Mastectomy, Xuan Ha Thi Nhu Jan 2017

The Effect Of Uncertainty Management Program On Quality Of Life Among Vietnamese Women With Post Mastectomy, Xuan Ha Thi Nhu

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aim of this study was to test the effect of the Uncertainty Management Program (UMP) on quality of life among Vietnamese women at 3-weeks post mastectomy. The quasi-experimental design with posttest only was conducted in 115 women with post mastectomy who were assigned in experimental group (n=57) and control group (n=58). The experimental group received the UMP and routine care while the control group received only routine care. Participants were assessed at 3-weeks post mastectomy using the modified Quality of Life Index Scale Vietnamese version with Cronbach's alpha coefficient of .81. It was found that at 3-weeks post-mastectomy, the …

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Patient's Perception And Satisfaction On Dental Implant Therapy By Postgraduate Dental Students And Experienced Dentists, Pat Vipattanaporn Jan 2017

Patient's Perception And Satisfaction On Dental Implant Therapy By Postgraduate Dental Students And Experienced Dentists, Pat Vipattanaporn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: To evaluate and compare patient's perception and satisfaction between patients who received dental treatment from postgraduate dental students and experienced dentists. Material and methods: This descriptive cross-sectional study was performed in patients who received the dental implant from Faculty of dentistry Chulalongkorn University. A data collection of patient's perception and satisfaction was done by questionnaire which was sent by mail. The questionnaire contained 23 statements including 7 statements of demographic data, 7 statements of perception, and 9 statements of satisfaction. The patient had to answer through the one-best-answer multiple choices and visual analog scale (VAS). Results: The 382 participants …

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Comparison Of The Accuracy Of Surgical Guided Template Produced From Intraoral Scan Technique And Model Scan Technique For Implantation Using Computer Assisted Implant Surgery, Prudsaporn Kiatkroekkrai Jan 2017

Comparison Of The Accuracy Of Surgical Guided Template Produced From Intraoral Scan Technique And Model Scan Technique For Implantation Using Computer Assisted Implant Surgery, Prudsaporn Kiatkroekkrai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: To compare the accuracy of implant position placed by CAIS technique using surgical guided template produced by intraoral scan and model scan. Materials and methods: Patients who needed single tooth implant restoration were enrolled in this study and devided into two groups randomly according to the surface scan technique (n=30 each group). Intraoral scans were done using the Trios Scanner (3shape). While, diagnostic casts were scanned by D900L lab scanner (3shape). The templates were design by the clinician using coDiagnostiX software (Dental Wings Inc.). Implant surgery were performed according to fully CAIS protocol. CoDiagnostiX software was used to compare …

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In Vitro Effect Of L-Ascorbic Acid On Gingival Fibroblast To Regulate Wound Healing., Tatcha Chaitrakoonthong Jan 2017

In Vitro Effect Of L-Ascorbic Acid On Gingival Fibroblast To Regulate Wound Healing., Tatcha Chaitrakoonthong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background and Objective: Besides closing the wound by intrinsic properties like migration and proliferation, gingival fibroblasts produce a major source of extracellular matrix especially collagen and play a major role in oral wound healing. Vitamin C or L-ascorbic acid has diverse functions in the body, including an essential role in hydroxylation reactions which is necessary for collagen formation. Whether L-ascorbic acid can promote gingival wound healing through inducing proliferation of fibroblasts is of our interest. The aim of this study is to evaluate the effect of L-ascorbic acid on gingival fibroblasts behaviors to promote wound healing in vitro. Materials and …

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Comparative Study Of In Vivo Bone Regeneration Using Chitosan/Dicarboxylic Acid Scaffold Implanted With Human Periodontal Ligament Cells, Teerawat Sukpaita Jan 2017

Comparative Study Of In Vivo Bone Regeneration Using Chitosan/Dicarboxylic Acid Scaffold Implanted With Human Periodontal Ligament Cells, Teerawat Sukpaita

Chulalongkorn University Theses and Dissertations (Chula ETD)

After tooth loss, a consequent atrophy of the alveolar process always takes place and adversely affect the reconstruction process especially by dental implant. A novel chitosan/dicarboxylic acid scaffold (CS/DA scaffold) has been developed and proven to be an excellent candidate material in bone tissue engineering. This study aimed to evaluate the effects of a CS/DA scaffold with and without seeded primary human periodontal ligament cells (hPDLCs) in mouse calvarial defect model. Eighteen mice were divided into 3 groups of 6 each. Four-millimeter calvarial defects were created on both side of parietal bone and implanted with either CS/DA scaffold or CS/DA …

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Induction Of S100a7 Expression From Mechanical Stress-Induced Human Dental Pulp Cells And Its Effect On Osteoclastogenesis, Hataichanok Charoenpong Jan 2017

Induction Of S100a7 Expression From Mechanical Stress-Induced Human Dental Pulp Cells And Its Effect On Osteoclastogenesis, Hataichanok Charoenpong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mechanical injuries of dental pulp tissues could lead to root resorption by osteoclast/odontoclast. S100A7 has been demonstrated to involve in inflammatory processes and potentially involved in bone destruction. However, the roles of S100A7 in dental pulp have not been reported. Therefore, this this study aimed to investigate the effect of mechanical stress on S100A7 expression in human dental pulp cells (hDPCs) and to investigate the effect of S100A7 protein on osteoclast differentiation. HDPCs were stimulated with in vitro compressive loading. Cell viability was determined using MTT assay. Real-time PCR (qPCR) and ELISA were utilized to determine the mRNA and protein …

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The Application Of Prostacyclin In Promoting Angiogenesis Of Dental Pulp Tissue In A Three Dimensional Organ Culture System, Sonntana Seang Jan 2017

The Application Of Prostacyclin In Promoting Angiogenesis Of Dental Pulp Tissue In A Three Dimensional Organ Culture System, Sonntana Seang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Dental pulp vitality can be threatened by carious or iatrogenic exposure that can lead to pulp tissue loss. The formation of a vascular network is crucial to provide a suitable environment for tissue regeneration. Thus, promoting angiogenesis is essential for successful pulpal repair in regenerative endodontics therapy. Iloprost, a prostacyclin analog, promotes vascularization in several organs such as, heart, lung, and corneal. Previously, it has been demonstrated that, iloprost induced pulpal blood flow and enhanced dentin formation in a rat mechanical pulp exposure model. However, the angiogenic effect of iloprost on the human dental pulp vasculature remains unknown. Objective: …

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Differential Dendritic Cell Responses To Candida Species, Thu Nguyen Ngoc Jan 2017

Differential Dendritic Cell Responses To Candida Species, Thu Nguyen Ngoc

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mannan in the outermost layer of Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes. However, little is known how different mannan structures of common Candida species affect DC activation and T cell responses. Thus, this work compared the effects of mannans of Candida albicans, Candida tropicalis, Candida glabrata, Candida krusei, Candida parapsilosis and Candida dubliniensis on bone marrow-derived DC (BMDC) responses in mice. C. albicans and C. tropicalis mannan could not activate DC maturation, however, they induced IFN-gamma production which consequently provoked Th1 responses. C. …

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Cd103+ Tissue Resident Memory T Cells In Oral Lichen Planus, Phisut Amnuaiphanit Jan 2017

Cd103+ Tissue Resident Memory T Cells In Oral Lichen Planus, Phisut Amnuaiphanit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Oral lichen planus (OLP) is a chronic inflammatory disease that results from a disorder of infiltrating T cells. Tissue resident memory T cells (TRM cells) are CD103-expressing T cells that persist within previously inflamed oral mucosa in the long term to provide locally rapid defensive responses against encountered pathogens. They are also speculated to be associated with some diseases including OLP. This study aimed to determine the number and the proportion of CD103+ TRM cells in the OLP lesions as compared to the normal mucosa. Immunohistochemical study of CD3+, CD4+, CD8+ and CD103+ cells in 15 OLP tissues and 15 …

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