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2017

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Articles 41131 - 41134 of 41134

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Rapid Rhd Zygosity Determination Using Digital Pcr., Kelly A. Sillence, Amr J. Halawani, Wajnat A. Tounsi, Kirsty A. Clarke, Michele Kiernan, Tracey E. Madgett, Neil D. Avent Jan 2017

Rapid Rhd Zygosity Determination Using Digital Pcr., Kelly A. Sillence, Amr J. Halawani, Wajnat A. Tounsi, Kirsty A. Clarke, Michele Kiernan, Tracey E. Madgett, Neil D. Avent

School of Biomedical Sciences

BACKGROUND: Paternal zygosity testing is used for determining homo- or hemizygosity of RHD in pregnancies that are at a risk of hemolytic disease of the fetus and newborn. At present, this is achieved by using real-time PCR or the Rhesus box PCR, which can be difficult to interpret and unreliable, particularly for black African populations. METHODS: DNA samples extracted from 58 blood donors were analyzed using 2 multiplex reactions for RHD-specific targets against a reference (AGO1)(2) to determine gene dosage by digital PCR. Results were compared with serological data, and the correct genotype for 2 discordant results was determined by …

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Kinetic Measurements Of Di- And Tripeptide And Peptidomimetic Drug Transport In Different Kidney Regions Using The Fluorescent Membrane Potential-Sensitive Dye, Dis-C3-(3)., Othman A. Alghamdi, Nicola King, Graham L. Jones, Pierre D.J. Moens Jan 2017

Kinetic Measurements Of Di- And Tripeptide And Peptidomimetic Drug Transport In Different Kidney Regions Using The Fluorescent Membrane Potential-Sensitive Dye, Dis-C3-(3)., Othman A. Alghamdi, Nicola King, Graham L. Jones, Pierre D.J. Moens

School of Biomedical Sciences

Tri- and dipeptides are transported in the kidney by PEPT1 and PEPT2 isoforms. The aim of this study was to investigate differences in transport kinetics between renal brush border (BBMV) and outer medulla (OMMV) membrane vesicles (where PEPT1 and PEPT2 are sequentially available) for a range of di- and tripeptides and peptidomimetic drugs. This was accomplished through the use of the potential-sensitive fluorescent dye 3,3'-dipropylthiacarbocyanine iodide [DiS-C3-(3)]. BBMV and OMMV were prepared from the rat kidney using standard techniques. The presence of PEPT1 in BBMV and PEPT2 in OMMV was confirmed using Western blotting. Fluorescence changes were measured when extravesicular …

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Identification Of The Direct Regulon Of Ntca During Early Acclimation To Nitrogen Starvation In The Cyanobacterium Synechocystis Sp. Pcc 6803., Joaquín Giner-Lamia, Rocío Robles-Rengel, Miguel A. Hernández-Prieto, Mi Muro-Pastor, Francisco J. Florencio, Matthias E. Futschik Jan 2017

Identification Of The Direct Regulon Of Ntca During Early Acclimation To Nitrogen Starvation In The Cyanobacterium Synechocystis Sp. Pcc 6803., Joaquín Giner-Lamia, Rocío Robles-Rengel, Miguel A. Hernández-Prieto, Mi Muro-Pastor, Francisco J. Florencio, Matthias E. Futschik

School of Biomedical Sciences

In cyanobacteria, nitrogen homeostasis is maintained by an intricate regulatory network around transcription factor NtcA. Although mechanisms controlling NtcA activity appear to be well understood, its regulon remains poorly defined. To determine the NtcA regulon during the early stages of nitrogen starvation for the model cyanobacterium Synechocystis sp. PCC 6803, we performed chromatin immunoprecipitation, followed by sequencing (ChIP-seq), in parallel with transcriptome analysis (RNA-seq). Through combining these methods, we determined 51 genes activated and 28 repressed directly by NtcA. In addition to genes associated with nitrogen and carbon metabolism, a considerable number of genes without current functional annotation were among …

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Ab0115 Comparison Of The Bacterial Stool Microbiota In Established Psoriatic Arthritis (Psa) And Psoriasis (Psc) - Exploratory Analysis Of Pilot Data, M Castelino, M Tutino, J Moat, Uz Ijaz, R Parslew, A Al-Sharqi, Rb Warren, C Quince, P Ho, M Upton, S Eyre, A Barton Jan 2017

Ab0115 Comparison Of The Bacterial Stool Microbiota In Established Psoriatic Arthritis (Psa) And Psoriasis (Psc) - Exploratory Analysis Of Pilot Data, M Castelino, M Tutino, J Moat, Uz Ijaz, R Parslew, A Al-Sharqi, Rb Warren, C Quince, P Ho, M Upton, S Eyre, A Barton

School of Biomedical Sciences

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