Digital Commons Network^™

Biofunctionalized Capillary Flow Channel Platform Integrated With 3d Nanostructured Matrix To Capture Circulating Tumor Cells, Shashwat Banerjee, Ganesh Khutale, Vrushali Khobragade, Narendra Kale, Milind Pore, Govind Chate, Archana Jalota-Badhwar, Manoj Dongare, Jayant Khandare

Articles

Circulating tumor cells (CTCs) from peripheral blood account genetic information for cancer diagnosis and overall disease monitoring. Analysis of “liquid biopsy” holds immense promise as it may lead to new approaches for cancer treatment. The study reports effective and continuous flow microchannel system for isolating CTCs using transferrin conjugated 3D matrix synthesized by crosslinking polyethylene glycol-Fe3O4 nanostructures for rapid and efficient capturing of CTCs. The platform provides option of using multiple microchannel units in series that can influence higher cell-capture efficiency due to increasing cell-substrate contact frequency. CTCs are captured with high efficiency even at low concentration of target cells …

Design, Synthesis, Characterization And Evaluation Of Near Infrared Bodipy Dyes For Various Applications, Qianli Meng

LSU Doctoral Dissertations

4,4-Difluoro-4-bora-3a,4a-diaza-5-indacene (BODIPY) have drawn a large number of research attention, for its numerous useful application in the fields of material and biology. Small modification of this well-known fluophore has been shown to optimize its photophysical and chemical properties. This work mainly focuses on functionalization of BODIPY cores with halogenated atoms, particularly iodine, and their subsequent post-functionalization reactions, mainly cross-coupling reaction and substitutions, for various further applications. Chapter 1 of this dissertation gives a general overview of BODIPYs including their synthetic strategies, post-functionalization, and how BODIPYs are utilized in many biological applications. Chapter 2 mainly focuses on the synthesis and characterization …

Isolation And Structural Elucidation Of Novel Bioactive Natural Products From Marine Organisms Of The Western Atlantic Ocean, Long Zhang

Electronic Theses and Dissertations 2010 - 2019

The aim of this dissertation was to elaborate the exploration of biologically active secondary metabolites from the marine sponge Cacospongia cf. linteiformis collected from the Bahamas and the soft coral Briareum asbestinum collected from two different sites in Florida State, Boca Raton and Dry Tortugas. In chapter one, a review on previous chemical and biological studies of the marine sponge C. cf. linteiformis and soft coral B. asbestinum is provided. Particular attention is given to spongianolides and briarellins, two important classes of compounds isolated from C. cf. linteiformis and B. asbestinum, respectively, and their structural features and diverse bioactivities. In …

University Of Texas At Tyler Course Catalog, 2017 - 2018, University Of Texas At Tyler

Catalogs

No abstract provided.

An Investigation Into Spike-Based Neuromorphic Approaches For Artificial Olfactory Systems, Anup Vanarse, Adam Osseiran, Alexander Rassau

Research outputs 2014 to 2021

The implementation of neuromorphic methods has delivered promising results for vision and auditory sensors. These methods focus on mimicking the neuro-biological architecture to generate and process spike-based information with minimal power consumption. With increasing interest in developing low-power and robust chemical sensors, the application of neuromorphic engineering concepts for electronic noses has provided an impetus for research focusing on improving these instruments. While conventional e-noses apply computationally expensive and power-consuming data-processing strategies, neuromorphic olfactory sensors implement the biological olfaction principles found in humans and insects to simplify the handling of multivariate sensory data by generating and processing spike-based information. Over …

Studies On Development In Euphilomedes Ostracods: Embryology, Nervous System Development, And The Genetics Of Sexually Dimorphic Eye Development, Kristina Koyama

University of the Pacific Theses and Dissertations

Model organism studies have been fundamental in understanding evolutionary and developmental biology. However, non-model organisms present opportunities to study unique characteristics and as comparisons to model organisms, leading us toward broader and more relevant perspectives on diversity. The Euphilomedes genus of ostracods is an example of a non-model group with potential for evolutionary and developmental studies.

Ostracoda is an ancient, basally branching lineage of Crustaceans with a complete and prodigious fossil record. Despite the group’s promise for evolutionary studies, much remains unknown about the basic biology of this clade. There are a limited number of embryogenesis studies in Ostracoda; here, …

A Review Of Random Matrix Theory With An Application To Biological Data, Jesse Aaron Marks

Masters Theses

"Random matrix theory (RMT) is an area of study that has applications in a wide variety of scientific disciplines. The foundation of RMT is based on the analysis of the eigenvalue behavior of matrices. The eigenvalues of a random matrix (a matrix with stochastic entries) will behave differently than the eigenvalues from a matrix with non-random properties. Studying this bifurcation of the eigenvalue behavior provides the means to which system-specific signals can be distinguished from randomness. In particular, RMT provides an algorithmic approach to objectively remove noise from matrices with embedded signals.

Major advances in data acquisition capabilities have changed …

Imaging Bioturbation In Supratidal Carbonates: Non-Invasive Field Techniques Enhance Neoichnological And Zoogeomorphological Research, San Salvador, The Bahamas, Karen Kopcznski, Ilya Buynevich, H. Allen Curran, Jon Caris, Jonathan Nyquist

Geosciences: Faculty Publications

A case study in unconsolidated carbonates on San Salvador Island, The Bahamas, utilized high-frequency (800 MHz) georadar imaging to augment existing methodologies (burrow counts and measurements, casting) in brachyuran bioturbation research (Ocypode quadrata and Gecarcinus lateralis), and as part of a new dataset characterizing blue land crab (Cardisoma guanhumi) burrows. Non-invasive techniques such as ground-penetrating radar (GPR) can complement traditional field surveys aimed at quantifying mesoscale bioturbation in modern settings. These methods can establish diagnostic features for tracemaker identification and refine existing ichnofacies models. Drone-mounted aerial coverage provided the first high-resolution images of the micro-topography and …

Effects Of Dredging-Related Pressures On Critical Ecological Processes For Organisms Other Than Fish Or Coral. Report Of Theme 9 - Project 9.1 Prepared For The Dredging Science Node, Jessie Short, Matthew Fraser, Dianne Mclean, Gary Kendrick, Maria Byrne, Julian Caley, Doug Clarke, Andy Davis, Paul Ertemeijer, Stuart Field, Sam Gustin-Craig, John Huisman, John Keesing, Mick Keough, Paul Lavery, Ray Mansini, Kathryn Mcmahon, Kerrie Mergersen, Michael Rasheed, John Statton, Jim Stoddart, Paul Wu

Research outputs 2014 to 2021

This study reviewed environmental windows for organisms other than corals and fish such as sessile invertebrates, macroalgae and seagrasses. Environmental windows are periods of critical importance to the life history of a marine species, like reproduction and recruitment.

Aims

• To review the scientific literature on the timing and spatial extent of critical ecological processes relevant to tropical WA for marine primary producers and invertebrates (i.e. organisms other than coral and finfish) and the state of knowledge regarding potential effects of dredging on these key ecological processes. The review also had a particular focus on ecological processes in marine primary producers …

Diet Of The Bull Shark, Carcharhinus Leucas, And The Tiger Shark, Galeocerdo Cuvier, In The Eastern Pacific Ocean, Colombo Estupiñán-Montaño, José F. Estupiñán-Ortiz, Luis G. Cedeño-Figueroa, Felipe Galvan Magaña, Carlos J. Polo-Silva

Turkish Journal of Zoology

This study presents information on the diet of two shark species, Carcharhinus leucas and Galeocerdo cuvier, that inhabit the southeastern Pacific Ocean. The stomachs were collected from October 2003 to July 2005 in Ecuador. Stomachs of 41 C. leucas and six G. cuvier were analyzed. According to the index of relative importance (%IRI), the most important prey for C. leucas were fishes: family Ophichthidae (13.41%), Tylosurus pacificus (9.79%), Katsuwonus pelamis (4.54%), and fish remains (44.81%). G. cuvier, for its part, consumed squids: Ancistrocheirus lesueuri (45.14%), Pholidoteuthis boschmaii (7.81%), and Octopoteuthis spp. (5.17%), as well as turtles: Caretta caretta (9.7%), Lepidochelys …

The Phenology Of Pulvinaria Floccifera Westwood (Hemiptera: Coccomorpha: Coccidae), A New Invasive Pest On Ornamentals Outdoors In Poland, Bozena Lagowska, Katarzyna Golan, Katarzyna Kmiec, Izabela Kot, Edyta Gorska Drabik, Katarzyna Goliszek

Turkish Journal of Zoology

The phenology of Pulvinaria floccifera infesting common holly was studied from June 2009 to October 2011 in Central Poland. Live specimens were most abundant on the leaves in July and August, while dead individuals were most numerous in winter and early spring each year. The scale completed one generation per year in the field and both the second and third instar nymphs overwintered. Following the spring molting to the 3rd instar or to the young adult females were observed in April and early May. The young adult females were first recorded in the second 10-day period of May. Oviposition occurred …

Human Serine Racemase Structure/Activity Relationship Studies Provide Mechanistic Insight And Point To Position 84 As A Hot Spot For Β-Elimination Function, David L. Nelson, Greg A. Applegate, Matthew L. Beio, Danielle L. Graham, David B. Berkowitz

Department of Chemistry: Faculty Publications

There is currently great interest in human serine racemase, the enzyme responsible for producing the NMDA co-agonist D-serine. Reported correlation of D-serine levels with disorders including Alzheimer’s disease, ALS, and ischemic brain damage (elevated D-serine) and schizophrenia (reduced D-serine) has further piqued this interest. Reported here is a structure/activity relationship study of position Ser⁸⁴, the putative re-face base. In the most extreme case of functional reprogramming, the S84D mutant displays a dramatic reversal of β-elimination substrate specificity in favor of L-serine over the normally preferred L-serine-O-sulfate (~1200-fold change in k_cat/K_{m …}

Nitroxide-Based Macromolecular Contrast Agents With Unprecedented Transverse Relaxivity And Stability For Magnetic Resonance Imaging Of Tumors, Hung V.T. Nguyen, Qixian Chen, Joseph T. Paletta, Peter Harvey, Yivan Jiang, Hui Zhang, Michael Boska, M. Francesca Ottaviani, Alan Jasanoff, Andrzej Rajca, Jeremiah A. Johnson

Andrzej Rajca Publications

Metal-free magnetic resonance imaging (MRI) agents could overcome the established toxicity associated with metal-based agents in some patient populations and enable new modes of functional MRI in vivo. Herein, we report nitroxidefunctionalized brush-arm star polymer organic radical contrast agents (BASP-ORCAs) that overcome the low contrast and poor in vivo stability associated with nitroxide-based MRI contrast agents. As a consequence of their unique nanoarchitectures, BASP-ORCAs possess per-nitroxide transverse relaxivities up to ∼44-fold greater than common nitroxides, exceptional stability in highly reducing environments, and low toxicity. These features combine to provide for accumulation of a sufficient concentration of BASP-ORCA in murine subcutaneous …

Biophysical Evidence For Intrinsic Disorder In The C-Terminal Tails Of The Epidermal Growth Factor Receptor (Egfr) And Her3 Receptor Tyrosine Kinases, Theodore R. Keppel, Kwabena Sarpong, Elisa M. Murray, John Monsey, Jian Zhu, Ron Bose

Open Access Publications

No abstract provided.

A Putative Causal Relationship Between Genetically Determined Female Body Shape And Posttraumatic Stress Disorder, Laura J. Bierut, John P. Rice, Nancy L. Saccone, Et Al

Open Access Publications

No abstract provided.

Synthetic (P)Ppgpp Analogue Is An Inhibitor Of Stringent Response In Mycobacteria, Kirtimaan Syal, Kelly Flentie, Neerupma Bhardwaj, Krishnagopal Maiti, Narayanaswamy Jayaraman, Christina L. Stallings, Dipankar Chatterji

Open Access Publications

No abstract provided.

Molecular Detection Of Nosema Spp. And Black Queen-Cell Virusin Honeybees In Van Province, Turkey, Beki̇r Oğuz, Zeynep Karapinar, Ender Di̇nçer, Mustafa Serdar Değer

Turkish Journal of Veterinary & Animal Sciences

This study was planned to determine the prevalences of the Nosema spp. and the black queen-cell virus (BQCV) among honeybees (Apis mellifera) raised in the province of Van by PCR and to determine the molecular characteristics of the determined isolates. A total of 260 adult worker bees from 26 colonies at 5 apiary locations belonging to the province of Van in April and May 2015 were collected for this reason. Samples were examined microscopically. In the case of positivity, spore identification was done by multiplex PCR. Reverse transcription/PCR analysis (RT/PCR) was carried out for the BQCV analysis. At the end …

Bibliometric Analysis Of Research Literature On Piper Betle, Dr. A. Thirumagal, A. Vanitha, M. Mani

Library Philosophy and Practice (e-journal)

Betel leaf plays an important role in ancient civilization. Chewing betel leaves with areca nut was pointed out in the pre-historic books. In 13^th century, Marco Polo mentioned about the betel chewing among kings and nobles in India. Betel and areca nut plays an important role in Indian Culture, especially among Hindus. On that basis of idea the keyword of “Piper betle” or betel was collected from the Web of Science. This study is limited for the period 1997- 2016 with Bibexcel and Pajek tool. Scientists are so much interested to publish the research immediately in the journal article. …

Fluorescent Probe Development For Fructose Specific Transporters In Cancer, Joseph Fedie

Dissertations, Master's Theses and Master's Reports

Carbohydrate transporters or GLUTs of the major facilitator superfamily (MFS) are responsible for transporting sugars into the cell and have been of research interest for decades. Disruptions, mutations, and over-activations of GLUTs have been linked to a number of major diseases including cancer, obesity, and diabetes. Differentiating between transporters is incredibly difficult due to highly conserved structures, and so specific targeting between transporters has proven a complex challenge. GLUTs are highly flexible in their conformations however exactly what will and will not pass through the transporter is ambiguous at best, and many attempt to target these transporters have failed.

In …

Advanced Analytical Strategies To Determine Biomedical Parameters For Medical Diagnostics, Drug Delivery, And Therapy, Sisi Chen

Doctoral Dissertations

"Biomedical parameters are critical for diseases prognosis, diagnosis and therapy. Many research groups have dedicated their studies to develop analytical instrumentation and apply analytical methods to determine biomedical parameters that have the potential to help with disease control and increase public health. This dissertation focuses on three major aspects of analytical strategies development and applications: 1) detection of pH changes caused by nanotoxicity (induced by TiO₂ nanoparticles) using newly developed micro-pH sensor, 2) quantification of renal cell carcinoma (RCC) biomarkers by high-performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS), and 3) nuclear magnetic resonance (NMR) studies of porous wall …

The Human Mitochondrial Chaperonin: It Takes Two Single-Rings To Tango, Adrian Sergio Enriquez

Open Access Theses & Dissertations

The human mitochondrial chaperonin is a macromolecular machine that catalyzes the proper folding and assembly of newly imported mitochondrial proteins into their biologically active state. It is composed of two proteins from the highly conserved heat shock protein family, hsp10 and hsp60, that assemble into large oligomeric complexes responsible for mediating the folding of non-native polypeptides in an ATP dependent manner. In addition to its innate role in protein folding, human mitochondrial hsp60 has been implicated in numerous moonlighting cellular activities that have been linked to diseases conditions such as cancer and neurodegeneracy. In light of its cellular importance, the …

Nest-To-Surf Mortality Of Loggerhead (Caretta Caretta) Sea Turtle Hatchlings On Florida’S East Coast In 2016, Victoria Erb

Electronic Theses and Dissertations 2010 - 2019

Worldwide, sea turtles are especially vulnerable immediately after emerging from nests. Many monitoring programs measure hatchling production from nest inventories. These inventories rarely account for mortality occurring post-emergence, leaving an incomplete estimate of hatchling production. This study addresses the nest-to-surf data gap for Florida’s east coast nesting assemblages of loggerhead sea turtles (Caretta caretta). Five locations were surveyed during the 2016 nesting season by using infrared time-lapse imagery, night vision optics, and track maps. Over all beaches, 7.6% of the observed hatchlings did not survive to reach the water. Mortality sources varied by location. Observed predators included: foxes, bobcats, yellow-crowned …