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2016

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The Tettigoniidae (Orthoptera: Ensifera): Phylogeny, Origins, And Leaf-Like Crypsis, Joseph D. Mugleston Jun 2016

The Tettigoniidae (Orthoptera: Ensifera): Phylogeny, Origins, And Leaf-Like Crypsis, Joseph D. Mugleston

Theses and Dissertations

Tettigoniidae (katydids) has more than 7200 species and is the largest family within the insect order Orthoptera. Their unique biology including leaf-like crypsis, acoustic signaling, and courtship rituals garners much of their academic attention. However, the taxonomy of katydids is chaotic and previous to these studies, little work had been done to decipher the phylogenetic relationships within this family. Without a robust phylogenetic framework, questions regarding the evolution of katydid disguises including the leaf-like crypsis cannot be addressed. This dissertation contains three chapters. Chapter 1 provides the first phylogenetic hypothesis focusing on Tettigoniidae. In this chapter we show a character …


Using Hadoop To Identify False Positives In Bacterial Strain Typing From Dna Fingerprints, Colin C. Adams Jun 2016

Using Hadoop To Identify False Positives In Bacterial Strain Typing From Dna Fingerprints, Colin C. Adams

Computer Science and Software Engineering

Pyroprinting is a novel technique used by the Department of Biological Sciences to obtain “fingerprints” from the DNA of E. coli isolates in order to categorize them into strains. To determine the number of false positives that occur in the pyroprinting process, isolates with the same pyroprints needed to be sequenced to see if their underlying alleles match. If they do match, this shows they are indeed the same strain and are a true positive. If the alleles don’t match, they are different strains and are a false positive. To do this 100 isolates with nucleotide identifiers were sequenced. Over …


Building A Freshwater Bacterial Flora Database For Remote Sensing Applications, Daniel Zimmerman, Laura Moore, J. A. Concha, N. Raqueño, Maryann Herman, Fernando Ontiveros Jun 2016

Building A Freshwater Bacterial Flora Database For Remote Sensing Applications, Daniel Zimmerman, Laura Moore, J. A. Concha, N. Raqueño, Maryann Herman, Fernando Ontiveros

Undergraduate External Publications

The identification and classification of microbial flora in bodies of fresh water has the potential of enhancing our understanding of this ecosystem and improving water management and bioremediation. This effort may be facilitated by the use of remote sensing technologies. For the last 3 years our undergraduate students have collected water samples in the Lake Ontario Rochester Embayment and Irondequoit Bay with the goal of constructing a database of bacterial species and water parameters (e.g. organic matter and chlorophyl content). Such a database is necessary to establish potential correlations between the presence of certain bacterial species and water parameters that …


A Novel Caloric Restriction-Like Mimetic Affects Longevity In Yeast By Reprogramming Core Metabolic Pathways, Jonathan I. Millen, Gregory Tombline, Bogdan Polevoda, Matan Rapaport, Bonnie Baxter, Andy Cannon, David S. Goldfarb Jun 2016

A Novel Caloric Restriction-Like Mimetic Affects Longevity In Yeast By Reprogramming Core Metabolic Pathways, Jonathan I. Millen, Gregory Tombline, Bogdan Polevoda, Matan Rapaport, Bonnie Baxter, Andy Cannon, David S. Goldfarb

Biology Faculty/Staff Publications

Glucose limitation is a simple intervention that extends yeast replicative lifespan (RLS) via the same pathway(s) thought to mediate the benefits of caloric restriction (CR) in mammals. Here we report on “C1”, a small molecule that mimics key aspects of CR. C1 was identified in a high throughput screen for drug-like molecules that reverse the RLS shortening effect of the sirtuin inhibitor and NAD+ precursor nicotinamide. C1 reduces the cellular dependence on glycolysis and the pentose phosphate pathway, even in the presence of glucose, and compensates by elevating fatty acid -oxidation to maintain acetyl-CoA levels. C1 acts either downstream of …


Carl Jung's Historic Place In Psychology And Continuing Influence In Narrative Studies And American Popular Culture, Emily S. Darowski, Joseph J. Darowski Jun 2016

Carl Jung's Historic Place In Psychology And Continuing Influence In Narrative Studies And American Popular Culture, Emily S. Darowski, Joseph J. Darowski

Swiss American Historical Society Review

Cart Gustav Jung was a Swiss-born psychiatrist who lived from the late nineteenth century well into the twentieth century. He founded the analytical psychology movement and is known for ideas such as the collective unconscious, archetypes, and one of the first conceptions of introversion and extraversion. The following paper explores Jung's influence on the field of psychology and other disciplines, postulating that his more lasting, but subtle influence exists outside his chosen field. This topic for exploration developed out of conversations between the two authors, one with an educational background in cognitive psychology, the other in American Studies. We draw …


The Male Anti-Circumcision Movement: Ideology, Privilege, And Equity In Social Media, Amanda Kennedy, Lauren M. Sardi Jun 2016

The Male Anti-Circumcision Movement: Ideology, Privilege, And Equity In Social Media, Amanda Kennedy, Lauren M. Sardi

Societies Without Borders

Social media has become a primary way in which various social movements may attempt to gain traction within larger frames of cultural discourse (Obar, Zube, and Lampe 2012). However, not all movements that profess human rights and equality goals are truly egalitarian in their orientation. Many men’s movements are ostensibly about gender equality but fall short of their claims because they fail to come to terms with issues of privilege (Kimmel 2013; Messner 1997, 1998). While the male anti-circumcision movement (sometimes referred to as the Intactivist movement) is less radically anti-feminist and has utilized social media to develop and maintain …


How-To Understand And Create Hip-Hop Art, Joshua Wolcott Jun 2016

How-To Understand And Create Hip-Hop Art, Joshua Wolcott

Graphic Communication

Typography is an essential component of Graphic Communication. The purpose of this study was to bridge typography with hip-hop art. To bridge the two disciplines, hip-hop art was presented in the context that it is actually practiced by these artists. The use of a questionnaire and a drawing lesson was intended to bring the subject within its true context. Presenting the art form in its true context enabled participants involved in the study to understand the art form with the perspective that the discipline, like typography, involves the use of lettering. Participants that were interested in drawing were able to …


Multidisciplinary Approaches And Challenges In Integrating Emerging Medical Devices Security Research And Education, Mehran Mozaffari Kermani, Reza Azarderakhsh, Mehdi Mirakhorli Jun 2016

Multidisciplinary Approaches And Challenges In Integrating Emerging Medical Devices Security Research And Education, Mehran Mozaffari Kermani, Reza Azarderakhsh, Mehdi Mirakhorli

Presentations and other scholarship

Traditional embedded systems such as secure smart cards and nano-sensor networks have been utilized in various usage models. Nevertheless, emerging secure deeply-embedded systems, e.g., implantable and wearable medical devices, have comparably larger “attack surface”. Specifically, with respect to medical devices, a security breach can be life-threatening (for which adopting traditional solutions might not be practical due to tight constraints of these often-battery-powered systems), and unlike traditional embedded systems, it is not only a matter of financial loss. Unfortunately, although emerging cryptographic engineering research mechanisms for such deeply-embedded systems have started solving this critical, vital problem, university education (at both graduate …


Biomimetic Seal Flipper Test Rig, Gordon D. Belyea, Laura Kawashiri, Dylan Rinker, Kurt Beske Jun 2016

Biomimetic Seal Flipper Test Rig, Gordon D. Belyea, Laura Kawashiri, Dylan Rinker, Kurt Beske

Mechanical Engineering

No abstract provided.


Characterization Of Nuclear Factor-Kappab Binding Sites In The Freshwater Snail, Biomphalaria Glabrata, Laura E. Deneckere Jun 2016

Characterization Of Nuclear Factor-Kappab Binding Sites In The Freshwater Snail, Biomphalaria Glabrata, Laura E. Deneckere

Lawrence University Honors Projects

Biomphalaria glabrata is an intermediate snail host for the digenean trematode, Schistosoma mansoni, which causes the human disease schistosomiasis. A lot of research has focused on the snail-schistosome interaction, especially in regards to the immune response of the snail. The nuclear factor-kappaB (NF-κB) pathway, which is involved in regulating the immune response, can be triggered by the Toll-like receptor (TLR) signaling pathway. However, not much is known about the specific molecular mechanisms regulating these responses. Both NF-κB and TLR homologues have recently been reported in B. glabrata so it is of great interest to determine if BgNF-κB can regulate …


“How Can You Live Without Your Kids?”: Distancing From And Embracing The Stigma Of “Incarcerated Mother”, Brittnie L. Aiello, Krista Mcqueeney Jun 2016

“How Can You Live Without Your Kids?”: Distancing From And Embracing The Stigma Of “Incarcerated Mother”, Brittnie L. Aiello, Krista Mcqueeney

Criminology Faculty Publications

This article examines how incarcerated mothers constructed moral identities in the face of stigma. Analyzing data from participant observation and 83 in-depth interviews with incarcerated mothers, we show that mothers claimed moral identities by distancing from the stigma of incarceration and/or embracing the identity of incarcerated mothers. Utilizing these strategies, women challenged the stigma of convicted felon/ bad mother and reinforced the assumptions that motherhood is compulsory and should be reserved for women with enough money and standing to give their children advantages. The implications for understanding motherhood as a mechanism of moral identity and social control are discussed.


Heterologous Expression Of A Rice Mir395 Gene In Nicotiana Tabacum Impairs Sulfate Homeostasis, Ning Yuan, Shuangrong Yuan, Zhigang Li, Dayong Li, Hong Luo Jun 2016

Heterologous Expression Of A Rice Mir395 Gene In Nicotiana Tabacum Impairs Sulfate Homeostasis, Ning Yuan, Shuangrong Yuan, Zhigang Li, Dayong Li, Hong Luo

Publications

Sulfur participates in many important mechanisms and pathways of plant development. The most common source of sulfur in soil –SO42−– is absorbed into root tissue and distributed into aerial part through vasculature system, where it is reduced into sulfite and finally sulfide within the subcellular organs such as chloroplasts and mitochondria and used for cysteine and methionine biosynthesis. MicroRNAs are involved in many regulation pathways by repressing the expression of their target genes. MiR395 family in Arabidopsis thaliana has been reported to be an important regulator involved in sulfate transport and assimilation, and a high-affinity sulphate transporter and three ATP …


Gray Area: Court Of Arbitration For Sport Says Neigh To Reconsidering Strict Liability For Equestrian Sport, Mary Zoeller Jun 2016

Gray Area: Court Of Arbitration For Sport Says Neigh To Reconsidering Strict Liability For Equestrian Sport, Mary Zoeller

Jeffrey S. Moorad Sports Law Journal (1994 - )

No abstract provided.


Calcium Phosphate As A Key Material For Socially Responsible Tissue Engineering, Vuk Uskoković, Victoria M. Wu Jun 2016

Calcium Phosphate As A Key Material For Socially Responsible Tissue Engineering, Vuk Uskoković, Victoria M. Wu

Pharmacy Faculty Articles and Research

Socially responsible technologies are designed while taking into consideration the socioeconomic, geopolitical and environmental limitations of regions in which they will be implemented. In the medical context, this involves making therapeutic platforms more accessible and affordable to patients in poor regions of the world wherein a given disease is endemic. This often necessitates going against the reigning trend of making therapeutic nanoparticles ever more structurally complex and expensive. However, studies aimed at simplifying materials and formulations while maintaining the functionality and therapeutic response of their more complex counterparts seldom provoke a significant interest in the scientific community. In this review …


Mononuclear Iron-(Hydro/Semi)Quinonate Complexes Featuring Neutral And Charged Scorpionates: Synthetic Models Of Intermediates In The Hydroquinone Dioxygenase Mechanism, Amanda E. Baum, Sergey V. Lindeman, Adam T. Fiedler Jun 2016

Mononuclear Iron-(Hydro/Semi)Quinonate Complexes Featuring Neutral And Charged Scorpionates: Synthetic Models Of Intermediates In The Hydroquinone Dioxygenase Mechanism, Amanda E. Baum, Sergey V. Lindeman, Adam T. Fiedler

Chemistry Faculty Research and Publications

Neutral and anionic scorpionate ligands have been employed to generate active-site models of hydroquinone dioxygenases (HQDOs). While the nonheme Fe center in nearly all HQDOs is coordinated to one Asp (or Glu) and two His residues, 1,2-gentisate dioxygenase (GDO) is unique in featuring a three His triad instead. A synthetic GDO model was therefore prepared with the neutral tris(4,5-diphenyl-1-methylimidazol-2-yl)phosphine (Ph2TIP) ligand. The gentisate substrate was mimicked with the bidentate ligand 2-(1-methylbenzimidazol-2-yl)hydroquinonate (BIHQ). X-ray diffraction analysis of the resulting complex, [Fe(Ph2TIP)(BIHQ)]OTf (1a), revealed a distorted square-pyramidal geometry. Structural and electrochemical data collected for 1a were compared to …


The Effects Of Forgiveness On Sustaining Sobriety In 12 Step Group Attendees, Rebecca Cornelius Jun 2016

The Effects Of Forgiveness On Sustaining Sobriety In 12 Step Group Attendees, Rebecca Cornelius

Electronic Theses, Projects, and Dissertations

There has been an increase of research in the area of forgiveness, particularly since the early 1980’s; however, there has been far less research done in the field of addiction and how forgiveness or the lack of forgiveness impact’s a person’s recovery. The profession of social work has a wide array of fields and services, and it is not uncommon for a social worker to work with a person abusing substances at some point in his or her career. While there are many aspects of treating and assisting client’s in their recovery, one topic that may not be discussed with …


Empowerment In Parent-Child Interaction Therapy (Pcit), Marlena M. Hernandez Jun 2016

Empowerment In Parent-Child Interaction Therapy (Pcit), Marlena M. Hernandez

Electronic Theses, Projects, and Dissertations

The purpose of this study is to explore empowerment in Parent‑Child Interaction therapy (PCIT). Research has shown that attachment issues and child maltreatment are serious social problems that may lead to risks of child abuse and children developing mental illnesses. Interventions, like PCIT have shown to decrease these risks by improving the parent‑child interactions by enhancing parent skill levels and by decreasing parent stress levels and child behaviors. The purpose of this study was to examine whether enhancing parent skills and decreasing parent stress levels would therefore increase parent empowerment. This study utilized a quantitative method to examine potential growth …


Challenges Of Social Workers Giving Mental Health Services To Juveniles Who Have Been Incarcerated For Drug Use, Nathalie Marie Jones Jun 2016

Challenges Of Social Workers Giving Mental Health Services To Juveniles Who Have Been Incarcerated For Drug Use, Nathalie Marie Jones

Electronic Theses, Projects, and Dissertations

This research examined challenges social workers face when delivering mental health services to juveniles who have been incarcerated due to drug use. Through the use of a web-based survey program a total of ten research questions were examined statistically. The research questions discussed what challenges social workers face when trying to administer services to juveniles who have been incarcerated for drug use and also what services are available to juveniles with or without a mental health problem who have been incarcerated for drug use. Questionnaires were sent out electronically, with a response rate of twenty-seven participants. SurveyMonkey® was used to …


Mental Health Practitioner Stigma, Attitude, And Belief: A Multidimensional Study On Marriage And Family Therapists, Clinical Social Workers, And Clinical Psychologists, Jessica Ann De La Rosa, Ruxandra Elena Tanase Jun 2016

Mental Health Practitioner Stigma, Attitude, And Belief: A Multidimensional Study On Marriage And Family Therapists, Clinical Social Workers, And Clinical Psychologists, Jessica Ann De La Rosa, Ruxandra Elena Tanase

Electronic Theses, Projects, and Dissertations

Existing stigma in the form of negative attitudes towards individuals with severe mental illness by mental health practitioners, has the potential to set barriers towards recovery. A survey of 72 mental health practitioners from three disciplines were surveyed, in an attempt to measure mental health practitioner attitudes towards individuals with severe mental illness, and how their attitudes impact their belief in client recovery. This was a quantitative study, based on two Likert Scale surveys and distributed both in paper form and using Survey Monkey. Participants were gathered through a snowball effect, and consisted of 42 social workers, 18 marriage and …


Effects Of Bisphenol-A And Styrene On Fertilization And Development Of The Purple Sea Urchin (Strongylocentrotus Purpuratus), Nicole C. Uibel, Nikki L. Adams, Jennifer Carroll, Sean Lema Jun 2016

Effects Of Bisphenol-A And Styrene On Fertilization And Development Of The Purple Sea Urchin (Strongylocentrotus Purpuratus), Nicole C. Uibel, Nikki L. Adams, Jennifer Carroll, Sean Lema

Master's Theses

Plastic waste and debris have greatly increased in the marine environment during the past 50 years. Not only do these plastics entangle and get ingested by marine mammals, turtles, and sea birds, but they also leach chemicals, such as bisphenol-A (BPA) and styrene into the aquatic environment. While some of these chemicals are known to be toxic, few studies have examined their effects on broadcast spawning marine invertebrates, specifically at environmentally relevant concentrations. Purple sea urchins, Strongylocentrotus purpuratus, from the central coast of California, were utilized to examine effects of BPA and styrene on fertilization success and early development of …


Genes Encoding Flower- And Root-Specific Functions Are More Resistant To Fractionation Than Globally Expressed Genes In Brassica Rapa, Naiyerah F. Kolkailah Jun 2016

Genes Encoding Flower- And Root-Specific Functions Are More Resistant To Fractionation Than Globally Expressed Genes In Brassica Rapa, Naiyerah F. Kolkailah

Master's Theses

Like many angiosperms, Brassica rapa underwent several rounds of whole genome duplication during its evolutionary history. Brassica rapa is particularly valuable for studying genome evolution because it also experienced whole genome triplication shortly after it diverged from the common ancestor it shares with Arabidopsis thaliana about 17-20 million years ago. While many B. rapa genes appear resistant to paralog retention, close to 50% of B. rapa genes have retained multiple, paralogous loci for millions of years and appear to be multi-copy tolerant. Based on previous studies, gene function may contribute to the selective pressure driving certain genes back to singleton …


The Effects Of Quorum Sensing And Temperature On The Soluble Proteome Of Vibrio Salmonicida, Christopher L. Massey Jun 2016

The Effects Of Quorum Sensing And Temperature On The Soluble Proteome Of Vibrio Salmonicida, Christopher L. Massey

Master's Theses

Vibrio salmonicida causes cold-water vibriosis in salmon populations around the world and causes financial damage to fisheries designed to farm these salmon. Very little is known about the physiology of how V. salmonicida causes disease and measures to contain vibriosis are restricted to either vaccinating individual fish against disease or administering antibiotics when an outbreak is detected. These procedures are costly and increase the risk for selection of antibiotic-resistant V. salmonicida strains. A recent reoccurrence of outbreaks in Norwegian fisheries provided incentive to better understand the virulence mechanisms of V. salmonicida. In this thesis, a proteomic approach was used to …


The Influence Of Exclusive Breastfeeding On Growth Trajectory From Birth To Year 5 In A Wic Cohort, Lindsey Arevalos Jun 2016

The Influence Of Exclusive Breastfeeding On Growth Trajectory From Birth To Year 5 In A Wic Cohort, Lindsey Arevalos

Master's Theses

OBJECTIVE. The impact of exclusively breastfeeding (EBF) on child body mass index (BMI) from birth through 60 months of age was investigated.

METHODS. 60,190 mothers and children attending Women, Infant and Children (WIC) clinics in the Santa Barbara and San Luis Obispo counties of California provided data on breastfeeding duration, maternal pre-pregnancy BMI, and child weight-for-length (WL) was measured at 3 month intervals until 5 years of age. Missing time points were interpolated in determining child BMI/WL z-score trajectories plotted on the Center of Disease Control (CDC) infant and child growth curves. Multivariate analysis of variance was used to contrast …


An Ergonomics Analysis Of Redundancy Effect In Touch Screen Design For The Aged Population, Sarah Riesenberg Jun 2016

An Ergonomics Analysis Of Redundancy Effect In Touch Screen Design For The Aged Population, Sarah Riesenberg

Master's Theses

Touch screen technology is rapidly increasing, and at the same time there is a shifting, aging population. As the percentage of the population over the age of 65 increases, adults of the age group are adopting smartphones and tablets more now than ever before. Although older adults are adopting touch screen devices, they face many challenges when interacting with said devices, such as not knowing how to navigate between pages, not knowing where to click for an action to occur, and the touch screen interface is often too sensitive or the buttons are not big enough. Furthermore, the challenges of …


Skewer: Sentiment Knowledge Extraction With Entity Recognition, Christopher James Wu Jun 2016

Skewer: Sentiment Knowledge Extraction With Entity Recognition, Christopher James Wu

Master's Theses

The California state legislature introduces approximately 5,000 new bills each legislative session. While the legislative hearings are recorded on video, the recordings are not easily accessible to the public. The lack of official transcripts or summaries also increases the effort required to gain meaningful insight from those recordings. Therefore, the news media and the general population are largely oblivious to what transpires during legislative sessions.

Digital Democracy, a project started by the Cal Poly Institute for Advanced Technology and Public Policy, is an online platform created to bring transparency to the California legislature. It features a searchable database of state …


Rearing Temperature Affects The Expression Of Proteins In The Adhesive Of The Striped Acorn Barnacle, Balanus Amphitrite, Melissa J. Daugherty Jun 2016

Rearing Temperature Affects The Expression Of Proteins In The Adhesive Of The Striped Acorn Barnacle, Balanus Amphitrite, Melissa J. Daugherty

Master's Theses

Barnacles are dominant hard–fouling organisms in marine waters. They attach to substrates by secreting a complex proteinaceous adhesive. Understanding the chemical composition of this multi–protein underwater adhesive and how it is affected by environmental variables, such as oceanic temperatures, is critical for developing nontoxic solutions to control biofouling. Previous experiments in our lab revealed an inverse relationship between critical removal stress (CRS) and temperatures at which barnacles were reared. Further investigations showed that this correlation is not attributed to differences in physical properties such as barnacle size or short–term changes in the viscosity of adhesive. Therefore, the observed effects may …


Characterizing Neurotransmitter Receptor Activation With A Perturbation Based Decomposition Method, Stephen Gregory Jue Jun 2016

Characterizing Neurotransmitter Receptor Activation With A Perturbation Based Decomposition Method, Stephen Gregory Jue

Master's Theses

The characterization of postsynaptic potentials, in terms of neurotransmitter receptor activation, is of clinical significance because information associated with receptor activation can be used in the diagnosis and study of neurological disorders. Single-unit recordings provide a method of measuring postsynaptic potentials in neurons using a microelectrode system, but yield no detailed information regarding the neurotransmitter receptors that contribute to the potential. To determine the types of neurotransmitter receptors that result in a compound postsynaptic potential from a microelectrode reading, decomposition of the potential is necessary. In this work, a perturbation-based decomposition method developed by R. Szlavik is evaluated for this …


Lower Scotts Creek Floodplain And Habitat Enhancement Project, Benjmain O. Cook Jun 2016

Lower Scotts Creek Floodplain And Habitat Enhancement Project, Benjmain O. Cook

Master's Theses

Scotts Creek, located in northern Santa Cruz County, maintains the southernmost persistent population of Central California Coast (CCC) Coho Salmon (endangered) in addition to CCC steelhead (threatened). Fisheries biologists believe overwinter mortality due to lack of refuge habitat is the primary factor limiting salmonid production. Instream rearing habitat may also be limiting, especially during drought years. The legacy effects of historic land use practices, including dredging, wood removal, and the construction of levees, continued to limit refuge and rearing opportunities. A restoration project was implemented to improve refuge and rearing opportunities for salmonids along lower Scotts Creek by removing portions …


Impact Of Assembly Bill 12 On Emancipated Foster Youth, Amber E. King Jun 2016

Impact Of Assembly Bill 12 On Emancipated Foster Youth, Amber E. King

Electronic Theses, Projects, and Dissertations

Assembly Bill 12 was passed into law for the purpose of preparing foster youth for the transition into adulthood and provided extended support services and transitional support up to the age of 21. This qualitative study focuses on the experiences and outcome of foster youth who are participating in extended care services. Key findings were identified in the areas of education, social and emotional support, and living arrangements. These key findings provide the social workers with a better understanding of the difficulties associated with the complexity involving the transition to young adulthood. The implications of key findings have resulted in …


Music Therapy Among The Elderly: What Social Workers Need To Know, Adriana Navarrete-Campos Jun 2016

Music Therapy Among The Elderly: What Social Workers Need To Know, Adriana Navarrete-Campos

Electronic Theses, Projects, and Dissertations

The purpose of this study was to gain a better understanding of music therapy among the elderly. This qualitative study utilized interviews as a means to extract themes from the experiences of music therapists who have provided music therapy to the elderly. Results were transcribed to written form. Qualitative analysis procedures were followed to identify themes and subthemes. Areas of particular interest to the social work profession were the emotional, social, physical, and mental benefits and challenges of music therapy among the elderly. Through the guidance of systems theory and the concept of holism, spiritual benefits and challenges were also …