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The Organization Of Technology In The Pine Hills Of Mississippi, Ronald W. Wise Jr.

Master's Theses

This thesis details the use of experimental flintknapping to better understand stone tool production and the organization of technology among Woodland period hunter-gatherers within the Pine Hills region of Mississippi. The Pine Hills region is characterized archaeologically by the presence of numerous sites consisting of flake scatters and little other material remains. Local tool stone resources consist of high grade chert in the form of small river cobbles, which restricts potential tool forms available to users.

Research for this project focused on the statistical analysis of debitage created during the experimental replication of stone tools using local chert cobbles. Special …

Reduction In Squamous Cell Carcinomas In Mouse Skin By Dietary Zinc Supplementation., Jin Sun, Rulong Shen, Morgan S. Schrock, James Liu, Xueliang Pan, Donald Quimby, Nicola Zanesi, Teresa Druck, Louise Y. Fong, Kay Huebner

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Inadequate dietary Zn consumption increases susceptibility to esophageal and other cancers in humans and model organisms. Since Zn supplementation can prevent cancers in rodent squamous cell carcinoma (SCC) models, we were interested in determining if it could have a preventive effect in a rodent skin cancer model, as a preclinical basis for considering a role for Zn in prevention of human nonmelanoma skin cancers, the most frequent cancers in humans. We used the 7,12-dimethyl benzanthracene carcinogen/phorbol myristate acetate tumor promoter treatment method to induce skin tumors in Zn-sufficient wild-type and Fhit (human or mouse protein) knockout mice. Fhit protein expression …

Editorial: Learn, By Listening To The Child In Neoliberal Schools, Debbie Sonu, Julie Gorlewski, Daniel Vallée

Publications and Research

This Special Issue for the Journal for Critical Education Policy Studies emerged out of a disappointed search for literature on the experiences of neoliberal education as spoken by children and youth. While there is no shortage of work on the reverberations of market ideology within the structures, policies, and practices of schooling in the United States, an overwhelming majority of this is discussed through the reflective hindsight of the adult. Thus, we as editors, purposefully designed this issue to address the marginalization of a constituency who we believe can illuminate the state of schooling in ways that we as adults …

The Effects Of Teachers’ Self-Efficacy & A Positive Learning Environment On Marginal Students In Mainstream High School Settings, Renée E. Thompson

All Theses And Dissertations

Research from data and anecdotal research from high school students informs us that many traditional high schools are not able to meet the academic, as well as the social and emotional needs of all students, particularly marginal students. The purpose of this research study was to determine how high schools may support marginal students in achieving success by the implementation of teacher self-efficacy and a positive classroom environment. In addition, the research will articulate the critical role of teachers and how their impact shapes the future of their students. The research questions posed will identify what interventions and strategies teachers …

Development Of Sybr Green I-Based Melting Curve Method For Her2i655v Polymorphism Detection In Breast Cancer, Bugi R. Budiarto, Wirsma A. Harahap, Desriani Desriani

Makara Journal of Health Research

Background: Currently available molecular method to detect HER2I655V polymorphism such as PCR-RFLP is hampered by the costly experimental method, and post-PCR treatment requirement that makes this technique is not meeting for high-throughput analysis purpose. In this study, we developed an accurate, simple, low cost and rapid test to detect polymorphism at HER2 gene using SBR Green I based-melting curve method. Methods: Two forward allele-specific primers and one common reverse primer were used then these primers were tested to discriminate known genotypes of genomic templates (GG type or AA type) and genomic samples retrieved from breast cancer patients. Results: Melting curve …

Evaluating The Relationship Between Derived Relational Responding, Verbal Operant Development, And Linguistic Structure: Correlating The Peak-E-Pa, The Ablls-R, And The Told-I:4, Bridget Munoz

Theses

The increasing prevalence of Autism Spectrum Disorder has produced a longstanding relevance for continued progressive measures towards a systematic approach to the treatment of deficient language repertoires. Current behavior analytic assessments, such as the Assessment of Basic Language and Learning Skills-Revised (ABLLS-R), have demonstrated utility in providing relative measures of the functional characteristics of an individual’s language and learner repertoire, as consistent with a traditional Skinnerian approach. Further assessments have been created under other existing theoretical frameworks, such as the Test of Language Development (TOLD), and the Promoting the Emergence of Advanced Knowledge Relational Training System (PEAK). Each assessment was …

Missing The Point Of The Practice-Based View, Philip Bromiley, Devaki Rau

Faculty Articles, Papers, and Other Scholarship

In this article, we address Jarzabkowski et al.’s strategy-as-practice criticism of Bromiley and Rau’s practice-based view as ignoring the “who” and “how” of practice implementation. Bromiley and Rau explicitly note that any statistical model under the practice-based view should consider mediating and moderating variables that depend on the specific practice and context but that the article would not attempt to identify such variables. Strategy-as-practice’s focus on the “who” and “how” of a practice are two of many such potential mediating or moderating variables. More fundamentally, strategy-as-practice scholars’ discomfort with the practice-based view may arise both from their different definitions of …

A Phase I Dose-Escalation Study Of The Braf Inhibitor Vemurafenib In Combination With The Mtor Inhibitor Everolimus In Subjects With Advanced Cancer, Javier Munoz

Dissertations and Theses (Open Access)

Vemurafenib has been approved in the United States for the treatment of relapsed or refractory BRAF mutation positive malignant melanoma and is being investigated in various other malignancies. The RAS/RAF/MEK/ERK (MAPK) pathway is critical to cell proliferation in many human cancers. The mTOR inhibitors are well known to exert profound anticancer effects across malignancies through inhibition of the PTEN/PI3K/AKT/mTOR (mTOR) pathway. We hypothesize that the toxicity profile of the combination of vemurafenib and everolimus will be well tolerated. The primary objective is to find the maximum tolerated dose (MTD) and the toxicity of the combination of vemurafenib and everolimus following …

Development Of An In Silico Kir Genotyping Algorithm And Its Application To Population And Cancer Immunogenetic Analyses, Howard Rosoff

Dissertations and Theses (Open Access)

Gene content determination and variant calling in the complex KIR genomic region are useful for immune system function analysis, pathogenesis and disease risk factor elucidation, immunotherapy development, evolutionary investigations, and human migration modeling. Sequence-specific oligonucleotide and sequence-specific primer PCR methods are the de facto standards for KIR presence/absence identification, but the current platforms are unsuitable for SNP calling, impractical for KIR typing large cohorts of DNA samples, and inapplicable for typing repositories in which sequence data, but not cells or cell analytes, are available. Alternative typing methods, such as in silico sequence-based typing, can address the problems associated with amplicon-based …

Investigating The Roles Of Δnp63 As A Suppressor Of Migration, Invasion, And Metastasis, Ramon E. Flores Gonzalez

Dissertations and Theses (Open Access)

Cancer is one of the leading causes of death and disease in the world. Considerable resources are spent to study and understand cancer, with the hope of developing new treatments and eventually cures that will help millions of people. Efforts to understand cancer are hindered by its inherent complexity and instability. Nonetheless, understanding the basics of tumor development and progression are the key to focused on studying the role of ΔNp63 in cancer, a p53 family member known to be involved in epithelial development, microRNA biogenesis, and stem cell maintenance. Using the strength of in vivo mouse models, we found …

Clinical Significance Of Homologous Recombination Deficiency Score Testing In Endometrial Cancer, Jean M. Hansen

Dissertations and Theses (Open Access)

Objectives: Homologous recombination deficiency (HRD) score is related to chemotherapy response in breast and ovarian cancers. The role of HRD is unknown in endometrial cancer. We examined the relationship between HRD score and survival in a cohort of endometrial cancer patients and with tumor growth using murine orthotopic models.

Methods: TCGA was queried to determine frequency and clinical significance of alterations in the HR pathway in endometrial cancer. 137 formalin-fixed paraffin-embedded endometrioid adenocarcinoma patient samples were tested for HRD score, microsatellite instability (MSI) and high mutation load (HML) using a next generation sequencing assay targeting the coding regions of 43 …

Development Of Rational Combination Therapy With Parp Inhibitors And Kinase Inhibitors In Tnbc, Wen-Hsuan Yu

Dissertations and Theses (Open Access)

Poly (ADP-ribose) polymerase inhibitors (PARPi) emerge as potential targeting drugs for BRCA-deficient cancers including triple negative breast cancer (TNBC). However, it has been reported that a subgroup of patients even with BRCA mutation fails to respond to PARPi in multiple clinical trials. In this study, we identified c-Met, a tyrosine kinase, phosphorylates PARP1 at Y907 and that the phosphorylation increases PARP1 activity, thereby rendering cancer cells resistant to PARPi. The combination of c-Met inhibitors (METi) and PARPi has a synergistic effect for c-Met overexpressed TNBC in vitro and in vivo. In addition to c-Met, through functional analysis, we found …

Development And Implementation Of An Anthropomorphic Head & Neck Phantom For The Assessment Of Proton Therapy Treatment Procedures, Daniela Branco

Dissertations and Theses (Open Access)

Proton therapy has been used to treat cancer for more than 50 years, and over the past decade, its use has grown rapidly. One of the main goals of modern radiation therapy is to deliver a high dose to the planning target volume (PTV) with minimal exposure and damage to the surrounding healthy tissue. Protons offer a unique advantage over photon radiotherapy in that they deposit dose over a finite range, in contrast to the more gradual energy deposition of photon and electron beams. At present, 23 proton centers are in operation in the United States and another 13 centers …

Define The Epigenetic Profiles And Subtype-Specific Genes Of Breast Cancer, Wenqian Li

Dissertations and Theses (Open Access)

Molecular profiling has identified 5 distinct subtypes of breast cancer, luminal A, luminal B, HER2-enriched, basal-like, and claudin-low breast cancer. These 5 subtypes correlate with hormone response, patient prognosis, and response to therapy. Although steady state gene expression patterns have been explored using expression microarrays, very little is known about the initial, disease-driving transcriptional changes in these cancers or epigenetic changes associated with the differential gene expression signatures. Defining these changes may provide new insights into the mechanisms by which these subtypes arise, as well as new avenues for breast cancer prevention, diagnosis, and treatment. Using Chromatin Immunoprecipitation sequencing and …

Interrogating Dux4 Mrna 3′ End Processing, Natoya J. Peart

Dissertations and Theses (Open Access)

Double Homeobox 4, Dux4, is the leading candidate gene for Facioscapulohumeral Dystrophy (FSHD). FSHD is the third most common muscular dystrophy, and is characterized by progressive muscle weakness primarily in the upper body. In individuals diagnosed with FSHD, Dux4 is inappropriately expressed in somatic cells due to two conditions. The first is hypomethylation of the subtelomeric D4Z4 repeats on chromosome 4. Each D4Z4 repeat on chromosome 4 is 3.3kb in length and contains the open reading frame for Dux4. Hypomethylation of the D4Z4 repeats primarily occurs due to contraction of the repeats from 11-100 (typical numbers in the healthy population) …

Tnf Signaling During Tissue Damage-Induced Nociceptive Sensitization In Drosophila, Juyeon Jo

Dissertations and Theses (Open Access)

Tumor necrosis factor (TNF) signaling is required for inflammatory nociceptive sensitization in both Drosophila and vertebrates. In Drosophila larval model of nociceptive sensitization, UV irradiation in results in epidermal apoptosis and thermal allodynia. TNF/Eiger is produced from dying epidermal cells and acts its receptor in nociceptive sensory neurons to induce thermal allodynia. Inhibition of TNF signaling results in attenuation of nociceptive sensitization whereas epidermal apoptosis still occurs in the absence of TNF. Major gaps in this model are the precise relationship between apoptotic cell death and production of TNF/Eiger, downstream signaling mediators for TNFR/Wengen, and target genes that alter nociceptive …

Defining The Functions Of Usp22 And Usp44 In Regulation Of H2bub1 Levels, Xianjiang Lan

Dissertations and Theses (Open Access)

Aberrant levels of histone ubiquitination are involved in various human diseases including neurodegenerative disorders and cancers. Particularly, Histone H2B monoubiquitination (H2Bub1) is highly associated with gene regulation in both normal cells and diseases. Many deubiquitinases (mainly USPs) are defined to regulate global H2Bub1 levels. However, how these USPs are regulated and how they contribute to diseases are not well understood.

USP22, part of the deubiquitination module (DUBm) in the SAGA complex, is a well-defined regulator of H2Bub1 levels. ATXN7, another crucial subunit of the SAGA DUBm, is involved in a neurodegenerative disease, spinocerebellar ataxia type 7 (SCA7), due to a …

An Automated Syringe Pump System For Improving The Reproducibility Of Dynamic Hyperpolarized Mri Phantoms, Harlee G. Harrison

Dissertations and Theses (Open Access)

AN AUTOMATED SYRINGE PUMP SYSTEM FOR IMPROVING THE REPRODUCIBILITY OF DYNAMIC HYPERPOLARIZED MRI PHANTOMS

Harlee Grace Harrison, B.S.

Advisory Professor: James Bankson, Ph.D.

Magnetic Resonance Imaging (MRI) is a powerful tool in the diagnosis of cancer due to its ability to provide good soft tissue contrast and image resolution without the use of ionizing radiation. The use of hyperpolarized pyruvate as a contrast agent for tumor metabolism during MR scans has the potential to provide information about tumor metabolism in vivo that is not available from traditional imaging measurements or any other method. Hyperpolarization is achieved through dynamic nuclear polarization. …

Epigenetic Modulation In Braf Mutated Metastatic Colorectal Cancer, Van Morris

Dissertations and Theses (Open Access)

Introduction: BRAF V600E mutations are associated with poor clinical outcomes for patients with metastatic colorectal cancer (mCRC). Unlike other tumors with the same mutation, BRAF inhibitors are ineffective as monotherapy. CRC tumors with BRAF V600E mutations are associated with global hypermethylation, which may turn off tumor suppressor gene expression. We studied demethylation in BRAF V600E mCRC to restore sensitivity to BRAF inhibitors.

Methods: Tumor databanks were investigated for genes differentially expressed according to BRAF mutation status to identify genes which may be particularly susceptible to epigenetic influence. Mouse xenograft models of BRAFV600E mCRC were treated with vemurafenib or azacitidine, alone …

The Immune Microenvironment Of Microsatellite Instable Endometrial Cancer, Janelle B. Pakish

Dissertations and Theses (Open Access)

Limited treatment options are available for patients with advanced and recurrent endometrial cancer (EC) should standard chemotherapy fail. Recent studies in other tumor types have shown that tumors with high microsatellite instability (MSI-H) have increased immunogenicity and response to immunotherapy treatments compared to microsatellite stable (MSS) tumors. Patients with MSI-H EC may also benefit from these therapies; however, the tumor immune microenvironment in MSI-H EC has not yet been well described.

In order to evaluate the immune microenvironment of MSI-H EC, multiple approaches were used, including analysis of large publically available datasets and detailed characterization of patient tumor samples. Uterine …

Employing T Cells For Antigen Presentation: Role Of Ny-Eso-1+ T-Apc Vaccine In Multiple Myeloma, Krina K. Patel

Dissertations and Theses (Open Access)

Introduction: Cellular therapy has shown great potential in early phase clinical trials with persistence of effector cells appearing to lead to improved outcomes. Combined immunotherapy with NY-ESO-1⁺ CAR T cells (TCR mimetic) and an NY-ESO-1 specific T-APC vaccine in myeloma patients may lead to enhanced anti-myeloma efficacy due to improved persistence of the CAR T cells.

Materials and Methods: Using the Sleeping Beauty transposon/transposase system, an NY-ESO-1⁺ TCR and CAR were created as effector cells. Vaccine was produced by electroporation of the NY-ESO-1 gene into T cells to make T cell antigen presenting cells (T-APC). Costimulatory …

Function And Mechanism Of Alkbh5 In N6-Methyl-Adenosine Rna Modification In Glioblastoma, Sicong Zhang

Dissertations and Theses (Open Access)

N⁶-methyl-adenosine (m⁶A) is the most prevalent internal chemical modification of mRNAs in eukaryotes. In mammals, m⁶A installed by m⁶A methyltransferases METTL3 and METTL14 is erased by two members of the AlkB family of nonheme Fe(II)/a-ketoglutarate (a-KG)-dependent dioxygenases, fat-mass and obesity associated protein (FTO) or ALKBH5. ALKBH5 affects nuclear RNA export and metabolism, gene expression and mouse fertility. To date, little is known about the biological significance of m⁶A in human cancer. We found that ALKBH5 is highly expressed in human glioblastoma stem cells which are resistant to conventional therapy and …

Novel Mechanisms Of Β-Adrenergic Signaling In Prostate Cancer Progression, Mohit Hulsurkar

Dissertations and Theses (Open Access)

Prostate cancer is the second leading cause of cancer death among American men. The American Cancer Society estimates that 180,890 men will be will be diagnosed with prostate cancer in 2016 in the USA. (http://www.cancer.org/cancer/prostatecancer/detailedguide/prostate-cancer-key-statistics). Androgen deprivation therapy (ADT) is the standard treatment for early stage prostate cancer. But most patients relapse with aggressive variants of prostate cancer, with survival time between 1-3 years. In order to develop cure for such aggressive variants of prostate cancer, our present understanding of the mechanisms underlying its progression needs to be advanced.

Recently, it has been found that activation of β-adrenergic signaling pathway …

Encapsulated Contrast Agent Markers For Mri-Based Post-Implant Dosimetry, Tze Yee Lim

Dissertations and Theses (Open Access)

Low-dose-rate prostate brachytherapy involves the implantation of tiny radioactive seeds into the prostate to treat prostate cancer. The current standard post-implant imaging modality is computed tomography (CT). On CT images, the radioactive seeds can be distinctively localized but delineation of the prostate and surrounding soft tissue is poor. Magnetic resonance imaging (MRI) provides better prostate and soft tissue delineation, but seed localization is difficult. To aid with seed localization, MRI markers with encapsulated contrast agent that provide positive-contrast on MRI images (Sirius MRI markers; C4 Imaging, Houston, TX) have been proposed to be placed adjacent to the negative-contrast seeds. This …

Morphological Systematics Of The Nightshade Flea Beetles Epitrix Foudras And Acallepitrix Bechyné (Coleoptera: Chrysomelidae: Galerucinae: Alticini) In America North Of Mexico, Anthony Martin Deczynski

All Theses

The flea beetle genera Epitrix and Acallepitrix are revised for America North of Mexico, building on a prior preliminary revision of the genus Epitrix by the author (Deczynski 2014). Four new species are described: Epitrix cuprea sp. nov., E. rileyi sp. nov., E. latifrons sp. nov., and E. vasinoda sp. nov., bringing the North American Epitrixfauna to a total of 26 species. A key is provided to adults of all species. A phylogenetic analysis is performed based on 44 external morphological characters and 30 genitalic characters. Acallepitrix nitens arose within the North American Epitrix, suggesting generic revision is required. The …

Hyberbranched Polymers As Biocompatible Oil Dispersants: Influence Of Salinity, Ph, And Concentration On Dispersion Effectiveness, Kristen Mae Carpenter

All Theses

The Deepwater Horizon oil spill in 2010 marked one of the biggest spills in history. Like many other oil spills, Corexit 9500 was used to attempt to prevent some of the environmental impacts of the spill. The abundant use of Corexit during this spill raised public awareness and called into question the dispersant’s toxicity and long-term impacts on the environment. These concerns brought to light the need to discover less toxic, more biodegradable oil dispersants. This research helps determine the feasibility of using hyperbranched polymers (HBP) as oil dispersants by comparing their oil dispersion effectiveness to that of the commonly …

Effect Of Forest Opening Characteristics, Prey Abundance, And Environmental Factors On Bat Activity In The Southern Appalachians, Jonathan Daniel Brooks

All Theses

Early successional habitat (ESH) is important for many wildlife species. Over the past century, land use changes have caused ESH to decline in hardwood forests of the eastern United States. The decline of ESH and ESH dependent wildlife has caused concern among land managers and scientists. Bats, which utilize ESH for foraging, are also a conservation concern, however little information is available on how ESH restoration affects bats. My objective was to determine how opening size, presence of edge, prey abundance, vegetation structure, and environmental factors affect bat activity in forest openings. In June-August 2014 and May-August 2015, I placed …

Fabrication And Characterization Of A Novel Porous Polymer Microsphere - Hydrogel Composite For Cell Therapy, Prathik Thulluri

All Theses

For patients with organ failure complications, organ transplantation is not alwaysan option due to a shortage of donors along with obstacles such as risk of pathogentransfer and the possibility of immune rejection. Tissue engineering approaches based oncell therapy provide a promising alternative that could potentially solve these problems.The success of cell therapy for tissue repair and regeneration requires reliable andefficient cell delivery methods. Currently, hydrogels are favored matrices for celldelivery because of their ability to be injected by minimally invasive techniques asviscous liquids and crosslinked in situ under mild conditions compatible with thehomogeneous incorporation of cells and bioactive molecules. However, …

Autologous Protein Solution Exhibits Potential To Mitigate Osteoarthritis (Oa) Progression In A Human Explant Co-Culture Model, Kathleen Emily Steckbeck

All Theses

Osteoarthritis (OA) is a debilitating and painful disease that affects upwards of one in every eight adults1,2. OA influences the entire joint, but is traditionally characterized by cartilage degradation3. OA is a multifactorial disease with genetic, biological, and biochemical consideration, but there has been a recent shift in scientific opinion that the pathological progression of OA stems from an inflammatory milieu produced by the feed-forward progressive pathway as evidenced by simultaneous cartilage and synovium co-culture4–7. Autologous protein solution (APS) poses as an intriguing potential OA therapy by possessing a high anti-inflammatory and anabolic mediator concentration profile that could potentially mitigate …

An Analysis Of The Putative Rhurm Pathway In Campylobacter Curvus, Marco Valera

All Theses

Recent genomic studies within the Epsilonproteobacteria have uncovered a potentially novel mechanism for chemotrophic respiration: the reverse Hydroxylamine Ubiquinone Redox Module (rHURM), a dissimilatory nitrate reduction pathway utilizing a hydroxylamine intermediate. Originally discovered in the chemoautolithotroph Nautilia profundicola, genes indicative of the rHURM pathway have been identified in several species of Campylobacter, including C. curvus. In the absence of classic nitrite reductase genes, a hydroxylamine oxidoreductase (hao) homolog encodes a periplasmic octoheme potentially capable of reducing nitrite, a product of periplasmic nitrate reductase (NapA), to hydroxylamine, which is then converted to ammonium by a putative hydroxylamine reductase …