Digital Commons Network^™

Synthesis And Characterization Of Imidazolo 3,1- Tetrakis (N-Phenylacetamidato) Dirhodium (Ii) And A Crystallographic Study Of A Copper And Two Molybdenum Model Cofactors, Gabriel I.G. Thompson

Electronic Theses and Dissertations

Imidazole was reacted with 3,1-tetrakis (N-phenlyacetamidato) dirhodium (II) to explore the chemistry of asymmetric dirhodium catalysts. The imidazolo 3,1-tetrakis (Nphenlyacetamidato) dirhodium (II) complex was synthesized and then characterized by Nuclear Magnetic Resonance and Ultraviolet-Visible spectroscopies as well as by single crystal X-ray Diffraction. Additionally, one copper and two molybdenum model cofactors were characterized by XRD to better understand their structure/function relationships. NMR results gave evidence of the formation of the 3,1-imidazole complex, and UV-Vis indicated that even in large excess imidazole was coordinated only to one axial site. The structure of the 3,1-imidazole complex was confirmed by XRD with the …

Characterization Of Pro-Inflammatory And Anti-Inflammatory Microglia In The Anterior Cingulate Cortex In Autism Spectrum Disorder, Aubrey N. Sciara

Electronic Theses and Dissertations

Autism spectrum disorder (ASD) is associated with functional abnormalities of the anterior cingulate cortex (ACC), a brain area that mediates social behavior. Given evidence of a role of inflammation in ASD, markers of pro-inflammatory and anti-inflammatory microglia were studied using postmortem ACC tissues from ASD and age-matched typically developed control donors. Gene expression levels of pro-inflammatory (CD68, HLA-DRA, IL1B, NOS2, PTGS2) and anti-inflammatory (ARG1, IGF1, MRC1, PPARG) microglial genes were measured using quantitative real-time PCR. Additionally, brain sections were immunohistochemically stained for a microglial marker. Expression levels of IGF1 were modestly higher, while the expression of …

Venom Peptides Lasioglossin Ii And Mastoparan B As Escherichia Coli Atp Synthase Inhibitors, Rafiat Ajoke Bello

Electronic Theses and Dissertations

The inhibitory effects on Escherichia coli ATPase activity by two venom peptides, lasioglossin II and mastoparan B. Membrane bound F₁F_O ATP synthase was isolated from E. coli strain pBWU13.4/DK8 and treated with varied concentrations of lasioglossin II and mastoparan B. Lasioglossin II caused very low inhibition of ATPase activity, but the inhibition profile of mastoparan B was suggestive of an interesting biological effect. A relatively shorter total length, a smaller net positive charge, and a reduced amphipathic character of both peptides, as compared to previously tested antimicrobial peptides, may account for the limited degree of inhibition observed …

Exploring Protective Factors Among Lesbians, Gays, And Bisexuals: A Framework For Psychological Well-Being And Relative Influence, Sheri Laduke

Electronic Theses and Dissertations

Lesbian, gay, and bisexual individuals must regularly navigate stigma, or social situations in which they are devalued because of their sexual orientation. The research has well established minority stress processes which link situations of stigma to reports of poor psychological well-being. However, protective factors leading to healthy psychological well-being are relatively understudied. This dissertation is a review of protective factors that have already emerged in the research and an assessment of these protective factors simultaneously to better understand how they influence psychological well-being. I recruited adult sexual minority participants using a comprehensive social media approach. I then tested mastery, problem-solving …

Classroom Environment: Content Analysis Examining Characteristics Of Classroom Environments That Affect Students' Academic Achievement, Lucinda Shaddock Bellamy

Electronic Theses and Dissertations

The purpose of this qualitative study was to extend the understanding of the characteristics of a classroom environment that impact students’ engagement in academics and therefore has the potential to positively impact student achievement scores. Data were collected through content analysis to analyze for reoccurring themes to assess how the characteristics of the classroom environment impact student’s achievement. Ten classrooms within the Kingsport City District were observed and analyzed for this study.

Six research questions guided this study, and qualitative data were analyzed for reoccurring themes. Findings from this study suggest that implementing certain characteristics in to the classroom environment …

Incremental Phylogenetics By Repeated Insertions: An Evolutionary Tree Algorithm, Peter Revesz, Zhiqiang Li

School of Computing: Faculty Publications

We introduce the idea of constructing hypothetical evolutionary trees using an incremental algorithm that inserts species one-by-one into the current evolutionary tree. The method of incremental phylogenetics by repeated insertions lead to an algorithm that can be used on DNA, RNA and amino acid sequences. According to experimental results on both synthetic and biological data, the new algorithm generates more accurate evolutionary trees than the UPGMA and the Neighbor Joining algorithms.

Mucosal Vaccination By Adenoviruses Displaying Reovirus Sigma 1, Eric A. Weaver, Zenaido T. Camacho, Matthew L. Hillestad, Catherine M. Crosby, Mallory A. Turner, Adam J. Guenzel, Hind J. Fadel, George T. Mercier, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

We previously developed adenovirus serotype 5 (Ad5) vectors displaying the sigma 1 protein from reovirus as mucosal vaccines. Ad5-sigma retargets to JAM-1 and sialic acid, but had 40-fold reduced gene delivery when compared to Ad5. While weaker at transduction, Ad5-sigma generated stronger T cell responses than Ad5 when used for mucosal immunization. New Ad5-fiber-sigma vectors were generated here by varying the number of fiber β-spiral shaft repeats (R) fused between fiber tail and the sigma. Ad5 virions encoding R3, R14, and R20 chimeras were rescued. Increasing chimera length led to their decreasing encapsidation of these proteins in the virions. Ad5-R3 …

Loss Of Exogenous Androgen Dependence By Prostate Tumor Cells Is Associated With Elevated Glucuronidation Potential, Brenna M. Zimmer, Michelle E. Howell, Qin Wei, Linlin Ma, Trevor Romsdahl, Eileen G. Loughman, Jennifer E. Markham, Javier Seravalli, Joseph J. Barycki, Melanie A. Simpson

Department of Biochemistry: Faculty Publications

Prostate epithelial cells control the potency and availability of androgen hormones in part by inactivation and elimination. UDP-glucose dehydrogenase (UGDH) catalyzes the NAD⁺-dependent oxidation of UDP-glucose to UDP-glucuronate, an essential precursor for androgen inactivation by the prostate glucuronidation enzymes UGT2B15 and UGT2B17. UGDH expression is androgen stimulated, which increases the production of UDP-glucuronate, and fuels UGT-catalyzed glucuronidation. In this study, we compared the glucuronidation potential and its impact on androgen-mediated gene expression in an isogenic LNCaP model for androgen dependent versus castration resistant prostate cancer. Despite significantly lower androgen-glucuronide output, LNCaP 81 castration resistant tumor cells expressed higher …

Polyandry In The Absence Of Fitness Benefits In A Species With Female-Biased Sexual Size Dimorphism, Marie Claire Chelini, Eileen A. Hebets

Eileen Hebets Publications

Most studies exploring the evolution of female mating systems focus on species in which females are either monandric (mate with a single male) or highly polyandric (mate with multiple males), but less is understood about variation in mating decisions within a species. How and why do females of a single species decide whether or not to copulate with additional mates? In this study we attempt to answer this question in the highly dimorphic crab spider, Mecaphesa celer, whose females may be either monandric or polyandric. We tested three hypotheses: (1) a female’s decision to remate is based on sequential mate …

Acceptability And Feasibility Of Point-Of-Care Cd4 Testing On Hiv Continuum Of Care In Low And Middle Income Countries: A Systematic Review, Minh D. Pham, Paul A. Agius, Lorena Romero, Peter Mcglynn, David Anderson, Suzanne M. Crowe, Stanley Luchters

Population Health, East Africa

Background: CD4 testing is, and will remain an important part of HIV treatment and care in low and middle income countries (LMICs). We report the findings of a systematic review assessing acceptability and feasibility of POC CD4 testing in field settings.

Methods: Electronic databases were searched for studies published in English between 2005 and 2015 that describe POC CD4 platforms. Studies conducted in LMICs and under field conditions outside a laboratory environment were eligible. Qualitative and descriptive data analysis was used to present the findings.

Results: Twelve studies were included, 11 of which were conducted in sub-Saharan countries and used …

A Review Of Glycemic Efficacy Of Liraglutide Once Daily In Achieving Glycated Hemoglobin Targets Compared With Exenatide Twice Daily, Or Sitagliptin Once Daily In The Treatment Of Type 2 Diabetes, Khalid Z. Alshali, Abdullah M. Karawagh

Saudi Medical Journal

Incretin-based therapies such as glucagon-like peptide-1 (GLP-1) receptor agonists (RA) and dipeptidyl peptidase-4 (DPP-4) inhibitors have gained prominence in recent years for the treatment of type 2 diabetes (T2D). Such therapies offer the potential to stimulate endogenous insulin activity in proportion to circulating glucose levels; thereby, lowering the risk of hypoglycemic episodes. The synthetic GLP-1 RA exenatide, the human GLP-1 RA liraglutide, and the DPP-4 inhibitor sitagliptin are the first agents in their respective classes to be approved for the treatment of T2D and their efficacy and safety has been studied extensively in clinical trials. This article reviewed the efficacy …

A Preliminary Exploration Of Ulnar Variance In Healthy Wrists At A Tertiary Hospital In Jeddah, Bayan A. Ghalimah, Reem L. Mimish, Khalid G. Khashoggi, Amre S. Hamdi

Saudi Medical Journal

Objectives: To gain preliminary insight by exploring ulnar variance changes in a Saudi-based sample. Methods: This 6-month (December 2013 to June 2014) cross-sectional study was conducted on a randomly selected healthy adult volunteers with a sample size of 104, at King Abdulaziz University Hospital, Jeddah, Saudi Arabia. Posteroanterior (PA), anteroposterior (AP), and PA grip views are taken. The variables of interest were the PA, AP, and PA fist measurements of both right and left wrists. An independent t-test was used to compare means between groups. Results: A total of 104 volunteers were recruited. Among 17 participants who had a negative …

Chronic Periodontitis And Smoking Prevalence And Dose-Response Relationship, Shahrukh Khan, Taimur Khalid, Kamran H. Awan

Saudi Medical Journal

Objectives: To determine the prevalence and dose-response relationship of chronic periodontitis among smokers in Pakistan. Methods: This is a cross-sectional study among participants seeking dental care in Karachi Medical and Dental College, Karachi, Pakistan. A total of 443 participants with a mean age of 44.3 (±6.5) participated in the study from April 2011 to December 2011. Males comprised 64.7%, and females comprised 35.2%. Participants were interviewed on social demographics and oral habits. Participants with shallow pockets (3.5-5.5 mm) and deep pockets (>5.5 mm) were considered suffering from chronic periodontitis. The characteristics of participants were assessed using frequency distribution for …

Cryptosporidium Parvum Among Coprolites From La Cueva De Los Muertos Chiquitos (600–800 Ce), Rio Zape Valley, Durango, Mexico, Johnica J. Morrow, Karl Reinhard

Karl Reinhard Publications

In the present study, 90 coprolites from La Cueva de los Muertos Chiquitos (CMC) were subjected to enzyme-linked immunosorbent assay (ELISA) tests for 3 diarrhea-inducing protozoan parasites, Entamoeba histolytica, Giardia duodenalis, and Cryptosporidium parvum, to determine whether these parasites were present among the people who utilized this cave 1,200–1,400 yr ago. These people, the Loma San Gabriel, developed as a culture out of the Archaic Los Caracoles population and lived throughout much of present-day Durango and Zacatecas in Mexico. The Loma San Gabriel persisted through a mixed subsistence strategy of hunting-gathering and agricultural production. The results of …

Development Of Genomic Resources For The Evaluation Of Red Snapper, An Emerging Species Candidate For Marine Aquaculture And Stock Enhancement, Adrienne Elise Norrell

Master's Theses

The northern red snapper (Lutjanus campechanus) is a highly targeted reef fish candidate for marine aquaculture and stock enhancement in the southern United States. This work aimed to develop genomic resources for the genetic management of aquaculture programs and to investigate population structure using high-throughput sequencing technologies. Eighty-four new microsatellite markers were developed through screening of Illumina paired-end sequencing reads. Microsatellite loci and Single Nucleotide Polymorphisms (SNPs) generated through Restriction Site Associated DNA (RAD) sequencing were assayed in 5 outbred full-sib families to construct a high-density linkage map of the red snapper genome. The map consists of 7,964 …

Growth And Reproduction Of Southern Flounder (Paralichthys Lethostigma) In The North-Central Gulf Of Mexico, Morgan Marie Corey

Master's Theses

Southern Flounder Paralichthys lethostigma is the most commonly harvested flatfish in the north-central Gulf of Mexico (GOM) and supports a major inshore recreational fishery, yet knowledge of the species’ life history is greatly limited. The objective of this research was to describe the growth and reproduction of Southern Flounder in the Mississippi stock. Fish were collected during September 2014 to March 2016 using primarily recreational fishing techniques. Otoliths (n = 313) were sectioned to estimate age, and multiple length-at-age models were fit to total length (TL, mm) and age estimate (y) data. Gonadal tissue samples (n = 221) …

The Intervening Sequence Of Coxiella Burnetii: Characterization And Evolution, Indu Warrier, Mathias C. Walter, Dimitrios Frangoulidis, Rahul Raghavan, Linda D. Hicks, Michael F. Minnick

Biology Faculty Publications and Presentations

The intervening sequence (IVS) of Coxiella burnetii, the agent of Q fever, is a 428-nt selfish genetic element located in helix 45 of the precursor 23S rRNA. The IVS element, in turn, contains an ORF that encodes a hypothetical ribosomal S23 protein (S23p). Although S23p can be synthesized in vitro in the presence of an engineered E. coli promoter and ribosome binding site, results suggest that the protein is not synthesized in vivo. In spite of a high degree of IVS conservation among different strains of C. burnetii, the region immediately upstream of the S23p start codon …

Pore Characterizations And Distributions Within Niagaran – Lower Salina Reef Complex Reservoirs In The Silurian Northern Niagaran Pinnacle Reef Trend, Michigan Basin, Agam Arief Suhaimi

Masters Theses

The Northern Niagaran Pinnacle Reef Trend (NNPRT) has generated significant oil and gas production in Michigan. The best reservoir rock in the Reef Trend reservoirs are from porous and permeable dolomite of the Guelph Dolomite. Low-to-non reservoir limestone occurs interstratified with reservoir dolomite in many locations. This study utilizes available cores data, thin section Petrographic Image Analysis (PIA), Mercury Injection Capillary Pressure (MICP) and a newly developed Niagaran Reef depositional model (Rine, 2015) to characterize the distribution of pore geometry within each dolomitized Niagaran Reef Complex (Brown Niagaran – Lower Salina Group) reservoir facies and lithofacies. This study shows that …

Approved For All Audiences: A Longitudinal Content Analysis Of The Portrayal Of Women In Movie Trailers, Brooke S. O’Neil

Masters Theses

Movie trailers are ever present in our society and impactful in the way society constructs views about various aspects of life. Trailers are unique in that they are specifically tailored and edited to entice audiences to buy tickets to the film. Further, prior research has indicated that in various forms of media, women are continuously underrepresented, disproportionately sexualized, stereotyped, and victimized. The present study examines the portrayal of women through a content analysis of 230 of the top grossing trailers across seven decades: 1950-2015. The research focuses on women’s representation, sexualization, gender roles, and violence. The analysis reveals that in …

Being With Bees: An Anthropological Study On Human-Animal Relations In Southern Beekeeping, Kori Nadine Armstrong

Master's Theses

This thesis investigates the complex and dynamic communicative relationship between beekeepers and their managed honey bee colonies, providing insight into the relationship between bees, their stewards, and the interface between nature and culture. It also helps unravel the ways in which this interspecies relationship changes a beekeepers’ perception of what it means to be human. Data collection features extensive participant observation with 21 semi-structured interviews with Southern beekeepers.

The interrelationship between communication and interaction, as well as diverse ways in which messages and cues manifest in the bee yard are explored through olfactory, optical, touch, and mechanical modalities. Variables including …

Current Medical Research Winter/Spring 2016, Richard J. Fehring

College of Nursing Faculty Research and Publications

No abstract provided.

N-Alkoxyphenylhydroxynaphthalenecarboxamides And Their Antimycobacterial Activity, Aidan Coffey, Et. Al.

Department of Biological Sciences Publications

A series of nineteen N-(alkoxyphenyl)-2-hydroxynaphthalene-1-carboxamides and a series of their nineteen positional isomers N-(alkoxyphenyl)-1-hydroxynaphthalene-2-carboxamides were prepared and characterized. Primary in vitro screening of all the synthesized compounds was performed against Mycobacterium tuberculosis H37Ra, M. kansasii and M. smegmatis. Screening of the cytotoxicity of the compounds was performed using human monocytic leukemia THP-1 cells. Some of the tested compounds showed antimycobacterial activity comparable with or higher than that of rifampicin. For example, 2-hydroxy-N-(4-propoxyphenyl)-naphthalene-1-carboxamide showed the highest activity (MIC = 12 µM) against M. tuberculosis with insignificant cytotoxicity. N-[3-(But-2-yloxy)phenyl]- and N-[4-(but-2-yloxy)phenyl]-2-hydroxy-naphthalene-1-carboxamide demonstrated high activity against all tested mycobacterial strains and insignificant cytotoxicity. …

Coevolution Of Cyanogenic Bamboos And Bamboo Lemurs On Madagascar, Daniel J. Ballhorn, Fanny Patrika Rakotoarivelo, Stefanie Kautz

Biology Faculty Publications and Presentations

Feeding strategies of specialist herbivores often originate from the coevolutionary arms race of plant defenses and counter-adaptations of herbivores. The interaction between bamboo lemurs and cyanogenic bamboos on Madagascar represents a unique system to study diffuse coevolutionary processes between mammalian herbivores and plant defenses. Bamboo lemurs have different degrees of dietary specialization while bamboos show different levels of chemical defense. In this study, we found variation in cyanogenic potential (HCNp) and nutritive characteristics among five sympatric bamboo species in the Ranomafana area, southeastern Madagascar. The HCNp ranged from 209±72 μmol cyanide* g^-1 dwt in Cathariostachys madagascariensis to no cyanide …

Retopologizing Mri And Diffusion Tensor Tractography Datasets For Real-Time Interactivity, R. Scott Riddle

Theses

Current technology allows MRI and other patient data to be translated into voxel-based 3D models for the purpose of visualization. However, these voxel models are extremely complex and are not suitable for rapid real-time manipulation. For true “on-the-fly” interactivity, polygon-based models must be hand-built using other methods and imported into a game engine.This project develops an algorithm to translate complex datasets into optimized models for real-time interactivity without sacrificing accuracy of the original imaging modality. A working prototype, ready for integration into game engines, has been built with brain tumor data exported from OSIRIX1 and 3D Slicer2 via Mudbox3, retopologized …

Community Structure And Production Of The Macrobenthos On Four Artificial Reefs In The Mississippi Sound In Relation To Substrate And Profile Type, Patrick Daniel Gillam

Master's Theses

In recent years, the Mississippi Department of Marine Resources (DMR) has made a concerted effort to enhance its coastal fishery resources by creating artificial reefs. Through this effort, 73 nearshore reefs have been created across the MS coast. Different substrate types used to construct MS artificial reefs include oyster shell and cement rubble. Two types of reef architecture used include high profile breakwater and low profile submerged structures. This study compared the benthic biota associated with oyster shell and cement rubble substrates among four artificial reefs representing high and low profile structures in summer 2011. Colonized benthic biota were quantified …

Decolonizing The Ya North: Environmental Injustice In Sherri L. Smith’S Orleans, Micah-Jade M. Coleman

Master's Theses

Young Adult (YA) dystopias, in recent years, have imagined a future world fueled by the overuse and misuse of technology, the advancement of science for human gain, as well as societies ruled by governments that govern based on their own self-interests and economic gain. Such novels have opened the door for discussion about how the present-day actions of societies can impact the future of the environment; yet many only focus their attention on societies in the North— regions considered “developed” by the western world. In her YA novel, Orleans (2014), Sherri L. Smith focuses attention on the aftermath of Hurricane …

"Black And White Together, We Shall Win": Southern White Activists In The Mississippi Civil Rights Movement, Olivia Bethany Moore

Master's Theses

During the Civil Rights Movement, Mississippi has often been characterized as a simple battle of white racists against black activists. Drawing heavily on oral histories, personal publications, and Mississippi Sovereignty Commission reports, this thesis examines the unconventional stories of white southerners who transcended the segregationist environments in which they were born. As southern white activism took many forms, this work offers biographical insights to three individuals who have received little scholarly attention: journalist P.D East, National Association for the Advancement of Colored People (NAACP) activist Buford Posey, and William Carey president Ralph Noonkester. While their contributions between 1950-1971 differed, being …

Effects Of Prebiotics On Gut Bacterial Communities And Healing Of Induced Colitis In Mice, Krystyn Elizabeth Davis

Master's Theses

Inflammatory Bowel Diseases (IBD) cause chronic inflammation of the gastrointestinal tract and debilitating symptoms in those suffering from the diseases. After inducing colitis in a mouse model using Dextran Sulfate Sodium (DSS), prebiotics inulin and oligofructose enriched inulin (OEI) were used as treatments to determine their effects on the gut microbial community, physiological healing process, and immune response in the mice after initial inflammation and before subsequent inflammation, or relapse. The treatment with inulin led to an increase in regulatory T cell number, but this increase was not as significant as the increase induced by the OEI. Inulin increased the …

An Assessment Of Number Representation In The Small-Eared Bushbaby (Otolemur Garnettii), Tiffany Aycia Woodard Baker

Master's Theses

In an effort to explain the underlying mechanisms of number representation, both the object-file and the approximate number system have been proposed. Despite the recognition in animals, both nonhuman and human, it remains unclear how numbers are represented cognitively or what system is employed. Furthermore, primate numerosity research has been almost exclusively conducted within haplorhine species (monkeys and apes). Within the strepsirhines (lemurs, lorises, and galagos), it has only been investigated in 15 diurnal and cathermal Malagasy primate species. No study to date has looked at this ability in either African strepsirhines or nocturnal primates. To examine the extent of …

Determining The Viability Of Recent Storms As Modern Analogues For North-Central Gulf Of Mexico Paleotempestology Through Sedimentary Analysis And Storm Surge Reconstruction, Joshua Caleb Bregy

Master's Theses

The northern Gulf of Mexico has been devastated by recent intense storms. Camille (1969) and Katrina (2005) are two notable hurricanes that made landfall in virtually the same location in Mississippi. However, fully understanding the risks and processes associated with hurricane impacts is impeded by a short and fragmented instrumental record. Paleotempestology could potentially use modern analogues from intense storms in this region to extend the hurricane record back to pre-observational time. Existing empirically based models can back-calculate surge heights over coastal systems as a function of transport distance, particle settling velocity, and gravitational acceleration. We collected cores in a …