Digital Commons Network^™

Cdk8 And Cdk19 As Novel Regulators Of Bmp4 Induced Emt In Cancer, Anne Elizabeth Serrao

Theses and Dissertations

Bone morphogenetic protein 2/4 (BMP2/4), members of the transforming growth factor β (Tgf-β) superfamily, play a dichotomous role in the progression of cancers through cell growth suppression and the enhancement of tumorigenesis in a variety of cancers. Cyclin dependent kinase 8 (CDK8), a protein kinase that regulates gene transcription, is involved in a variety of cancers including breast and pancreatic cancers as well as in mediating BMP2 signaling via its canonical Smad1 pathway. Our studies show that BMP4 induced EMT signals primarily though Smad1 and that Yap1, a transcriptional co-activator, is necessary for BMP4 induced EMT. Using a highly specific …

Some Extremal And Structural Problems In Graph Theory, Taylor Mitchell Short

Theses and Dissertations

This work considers three main topics. In Chapter 2, we deal with König-Egerváry graphs. We will give two new characterizations of König-Egerváry graphs as well as prove a related lower bound for the independence number of a graph. In Chapter 3, we study joint degree vectors (JDV). A problem arising from statistics is to determine the maximum number of non-zero elements of a JDV. We provide reasonable lower and upper bounds for this maximum number. Lastly, in Chapter 4 we study a problem in chemical graph theory. In particular, we characterize extremal cases for the number of maximal matchings in …

Investigating Structure-Activity Relationships Of Sl11 To Develop A Better Prostate Cancer Diagnostic, Rong Huang

Theses and Dissertations

Prostate cancer is now the second leading cause of cancer death in American men. Since many of the diagnostics either have high false results or risky, it’s important to have better diagnostics and earlier diagnosis of prostate cancer. Aberrant glycosylation is a hallmark of cancer. When cancer occurs, aberrant glycosylation occurs, therefore, glycosylation patterns may provide information about different cancer types and metastatic potential. In order to study and use these glycosylation patterns, the boronic acid functionalized synthetic lectins that can interact with glycans have been developed. The work described here focuses on 1) the structure activities of synthetic lectins …

Aging, Discourse, And Ideology, Julia Mckinney

Theses and Dissertations

This dissertation explores the language practices of members of the Andrus Center, a recreational senior center located in the southeastern United States. It specifically examines how “young-old” members, or those who had relatively recently made the transition to older identity, invoked and contested widely circulating ideologies of aging in the course of constructing their local age identities. Rather than treating age as an objective, individual characteristic, as commonly presumed in sociolinguistics, this study highlights the ways in which age identities were relationally and emergently coconstructed. Through analyses of interactional and ethnographic data collected over 18 months, I argue that mainstream …

The Association Between Physical Activity And Serum Immunoglobulin G (Igg) Antibodies Against Periodontal Bacteria, A. Paige Anderson

Theses and Dissertations

The purpose of this study is to add more research and evidence to the mechanisms surrounding periodontal disease, periodontal microorganisms, and physical activity. The main objective of this study is to evaluate and explore the association between physical activity and serum IgG antibodies, which are grouped into four distinct clusters, formed from species specific 19 periodontal antibody titers. This cross sectional study divided physical activity into three categories: adequately physically active, inadequately physically active, and inactive based on MET scores. Certain models were adjusted for confounders including age, sex, race/ethnicity, income-to-poverty ratio, years of formal education, smoking, alcohol intake, BMI, …

Neutering Neoliberalism: Masculinities And Gore Capitalism In Rubem Fonseca’S Crime Novels, David William Hancock

Theses and Dissertations

This study presents close readings of Rubem Fonseca’s Agosto (1990), A grande arte (1983), Bufo & Spallanzani (1985), and O seminarista (2009), to suggest they condemn Neoliberalism’s role in creating a global culture of violence, as they problematize its rhetoric of domination and uncover its heteropatriarchal, consumerist ideology, disguised as fact or ‘common-sense.’ The four chapters are divided according to the different theoretical concepts that accompany the four principal texts’ common critique of Neoliberal masculinity, as it functions to uphold the interdependent hierarchies of race, class and gender. Fonseca’s texts also imply the reader's’ complicity in a global culture of …

Utilization Of Satellite-Derived Salinity To Study Indian Ocean Climate Variability, Joseph Matthew D’Addezio

Theses and Dissertations

For several decades, researchers have explored Indian Ocean climate variability primarily using numerical models because of a lack of observations. Remote sensing technology has helped overcome this scarcity of observational data, but satellite-derived salinity has only been recently made available by the ESA’s Soil Moisture and Ocean Salinity (SMOS, operating since November 2009-present) and NASA’s Aquarius SAC-D (operated during June 2011-June 2015) satellites. Along with the Lagrangian in situ Argo floats array, these new datasets may be used to validate widely used numerical models, such as the HYbrid Coordinate Ocean Model (HYCOM), and also potentially observe new salinity phenomena that …

Sleep Disorder Trends, Epigenetic Markers, And Genetic Variation Of Circadian Genes In Adenomatous Polyp Formation, Melannie Alexander

Theses and Dissertations

Introduction: Sleep disturbances and sleep quality have fluctuated over time in the United States (US). Virtually every physiological process follows a circadian pattern, and cellular processes (i.e., cell proliferation, DNA damage response, and apoptosis) are often disrupted by sleep disturbances and other factors, potentially giving rise to adverse health outcomes such as cancer. Additionally, both genetic variation and epigenetic patterns within clock genes, which may affect sleep quality, also may increase one’s risk for developing cancer. The role of sleep disturbances in the formation of adenomas, precursor lesions to colorectal cancer (CRC), has been explored previously; however, little is …

Regular Expression Synthesis For Blast Two-Hit Filtering, Jordan Bradshaw

Theses and Dissertations

Genomic databases are exhibiting a growth rate that is outpacing Moore's Law, which has made database search algorithms a popular application for use on emerging processor technologies. NCBI BLAST is the standard tool for performing searches against these databases, which operates by transforming each database query into a filter that is subsequently applied to the database. This requires a database scan for every query, fundamentally limiting its performance by I/O bandwidth. In this dissertation we present a functionally-equivalent variation on the NCBI BLAST algorithm that maps more suitably to an FPGA implementation. This variation of the algorithm attempts to reduce …

Mislabeling Of Commercial Atlantic Cod (Gadus Morhua) Products In Spain, Joshua Helgoe

Theses and Dissertations

The mislabeling of commercial fish products is a pervasive, worldwide problem. Most consumers of seafood are unaware this issue directly affects them and can even have negative impacts on their health. Mislabeling occurs when a product’s label is inconsistent with its content. Although mislabeling can be unintentional, deliberate mislabeling is a more common trend to increase profits and/or bypass fishing regulations - a form of economic fraud. Unfortunately, oversight, enforcement, and research are vastly insufficient in relation to the global scale of the problem. In order to add to the small knowledge base on European mislabeling rates, determine if overfished …

Influence Of Built Environment On Physical Activity Outcomes Among African Americans In Community-Based Obesity Intervention Studies, Amanda Sue Thomas

Theses and Dissertations

More than sixty-five percent of people in the U.S. are considered overweight or obese. African-Americans in the U.S. have a higher risk of obesity than any other racial group. One way to reduce this statistic is physical activity. Recreational green spaces (parks) can serve as an avenue to complete the 150 min/wk of recommended physical activity for adults by the ACSM. Data from SISTAS and HEALS interventions, that recruited overweight/obese African-Americans from Columbia and Florence, SC, was used to assess the association of recreational green space (parks) around a residence and physical activity. Physical activity measures of RAPA questionnaire (self-report), …

Synthesis Attempts And Plausible Applications Of Dithiolated Ortho –Carboranyl Transition Metal Complexes And Synthesis Of Bulky Terphenyls, Surendra Bahadur Karki

Theses and Dissertations

The reaction of coordination compounds of palladium, rhodium, ruthenium and nickel with thiolated ortho-carborane derivatives- 1,2-dimercapto-o-carborane (1,2-(HS)2-1,2- C2B10H10 ) and 9,12- dimercapto-o-carborane (9,12-(HS)2-1,2- C2B10H10 ) and with carbornanyl dicarboxylate has been studied. To further investigate the chemistry of o-carboranyl dithiolate, both of the reported literature dithiols have been employed to study the reactivity with some of the above mentioned transition metal coordination compounds. The 1,2-dimercapto-o-carborane reacts with bis(triphenylphosphine)palladium(II) chloride [PdCl2(PPh3)2] to form a square planar bis(triphenylphosphine)palladium(II) carboranyl dithiolate (PdC2B10S2(PPh3)2) complex. With the inspiration from the reactivity of the dithiols and versatility of carboranyldithiolate ligand in organometallic chemistry, new avenue of …

Iv Prenatal Nicotine Exposure Modulates Α6, Α7, And Α4Β2 Nicotinic Acetylcholinergic Receptor Subunit Expression In Male And Female Neonatal And Adolescent Rat Offspring: An Autoradiographical Analysis, Amanda Morgan

Theses and Dissertations

Maternal smoke exposure produces long-term adverse cognitive and behavioral outcomes in offspring, including an increased likelihood of attention problems (e.g., attention deficit hyperactivity disorder; ADHD) and drug abuse. Preclinical research shows that gestational exposure to nicotine, the primary psychoactive compound in tobacco smoke, influences the neurodevelopment of attention and reward neuronal circuits. This study investigated hypotheses about five brain regions, to determine if prenatal nicotine (PN) exposure altered expression of nicotinic acetylcholine receptors (nAChRs). A low dose, intravenous nicotine (IV) exposure method was used to administer nicotine (0.05 mg/kg/injection) or saline, 3x/day on gestational days 8–21 (Treatment). Brain tissue was …

In The Belly Of The Whale: Archive And Access In Melville’S Moby-Dick, Garrett Urban

Theses and Dissertations

This thesis examines Melville’s depiction of archival anxiety as it appears in Moby-Dick. This narrative is fixated on the archive, brimming with motifs that equate whaling with the creation of an archive—and which associates the hunt for the whale with an obsession resembling Derrida’s vision of archive fever. As Melville depicts it, the archive is rooted in a fundamental anxiety concerning inaccessible and unknowable information. Ishmael constantly presents whales as physical repositories of information, attempting to archive the social and historical contexts of every scrap of flesh on them and every substance they produce. Yet his archival mission is perpetually …

Empowering The Enemy: The Cold War And The East-West Battle For Information Superiority, 1945 - 1969, Stan Trembach

Theses and Dissertations

This comparative study employs a historical perspective to narrow the gap in the existing knowledge of the origins of an information explosion phenomenon that dates back to the early decades of the twentieth century. It argues that the root cause of the unprecedented growth of the overall amount of documents was the rapid expansion of scientific and technical advances across the world and the subsequent spread of modern technologies, particularly those applied to scientific and technical information (STI).

This research is based on the premise that the thriving of the Soviet militaryindustrial complex went hand in hand with the rise …

Orange Juice Elegies, Jared Coffin

Theses and Dissertations

This is a creative thesis presented as a collection of poems written by Jared Coffin. This is the complete thesis submitted, defended, and approved as part of Jared’s completion of the requirements for earning the Master of Fine Arts in Creative Writing.

Bayesian Ensemble Of Regression Trees For Multinomial Probit And Quantile Regression, Bereket P. Kindo

Theses and Dissertations

This dissertation proposes multinomial probit Bayesian additive regression trees (MPBART), ordered multiclass Bayesian additive classification trees (O-MBACT) and Bayesian quantile additive regression trees (BayesQArt) as extensions of BART - Bayesian additive regression trees for tackling multinomial choice, multiclass classification, ordinal regression and quantile regression problems. The proposed models exhibit very good predictive performances. In particular, ranking among the top performing procedures when non-linear relationships exist between the response and the predictors. The proposed procedures can readily be applied on data sets with the number of predictors larger than the number of observations.

MPBART is sufficiently flexible to allow inclusion of …

The Influence Of Prenatal And Early Life Factors On Bmi Z Scores And The Risk Of Being Obese In Early Childhood, Junxiu Liu

Theses and Dissertations

Childhood obesity is a serious public health challenge. The underlying causes behind the rising levels of childhood obesity might be driven by prenatal and early life factors. Recently, several studies have examined the association between gestational weight gain (GWG) and the risk of obesity in the pediatric population. Findings on the association of both inadequate and excessive GWG with offspring obesity are inconsistent and vary by maternal prepregnancy body mass index (BMI) status. Inconsistent findings also exist for the association of GWG and BMIZ as a continuous outcome. Nevertheless, existing studies mainly focus on the BMIZ upper centiles using logistic …

Utilization Of Satellite-Derived Salinity For Enso Studies And Climate Indices, Caroline Mary Corbett

Theses and Dissertations

Conventionally, El Niño-Southern Oscillation (ENSO) is studied using sea surface temperature (SST) observations in the central and eastern equatorial Pacific Ocean. Recently, sea surface salinity (SSS) in the equatorial Pacific has been studied in relation to the phases of ENSO. Previously, SSS was observed in the equatorial Pacific using the Tropical Ocean Global Atmosphere (TOGA) / Tropical Atmosphere Ocean (TOA), the Argo float network, and Oceanic General Circulation Models (OGCM). The launch of NASA’s Aquarius Salinity Mission and ESA’s Soil Moisture Ocean Salinity (SMOS) opened a new era in which high resolution global near real-time SSS observations are readily available. …

Physical Property Control In Core/Shell Inorganic Nanostructures For Fluorescence And Magnetic Targeting Applications, Stephen K. Roberts

Theses and Dissertations

Nanomaterials show immense promise for the future in numerous areas of application. Properties that are unique from the bulk material and are tunable allow for innovation in material design. This thesis will focus on controlling the physical properties of core/shell nanostructures to enhance the utility of the materials.

The first focus is on the impact of different solvent mixtures during the shell growth phase of SILAR based core/shell quantum dot synthesis is studied. Gaining insight into the mechanism for SILAR growth of core/shell nanoparticles allows improved synthetic yields and precursor binding, providing enhanced control to synthesis of core/shell nanoparticles. The …

Finding Sanctuary In Sisterhood: A Middle School Literacy Group Critically Analyzes Race, Gender, And Size, Dywanna E. Smith

Theses and Dissertations

This critical qualitative study documented intersectionalities of race, gender, body image, and literacies as interpreted by five eighth grade Black female students during an eight-week after school literacy group. Ethnopoetic methodologies were used to represent findings and provide insights about how critical literacy engagements can be used to create discursive spaces where young Black girls describe and define these intersectionalities, how they are socialized into current beliefs, make meaning of beauty ideals portrayed in media, and interpret gender, obesity, and race. Through content analysis of field notes, audio recorded sessions, research partner interviews, researcher journals, and genre artifacts, this study …

Deciphering The Interacting Regions And Functions Of The Rad51d-Nono Protein Complex During Dna Damage Response, Latarsha Porcher

Theses and Dissertations

Genomic instability is one of the major components represented in the “Hallmarks of Cancer.” DNA interstrand crosslinks and double-stranded breaks are two of the most severe causes of genomic instability. Homologous recombination (HR) plays a major role in resolving both types of DNA lesions, requiring a homologous template and the RAD51 paralog complex of proteins. One member of this complex is the RAD51D ovarian cancer susceptibility gene product. To better elucidate RAD51D modifications and functions, several protein interaction screens were performed, and one of the novel proteins identified was NONO (a.k.a. p54nrb). Decreased expression of either RAD51D or NONO conferred …

Surface Chemistry And Transport Properties Of Ii-Vi Semiconductor Nanowires, Pravin Paudel

Theses and Dissertations

Semiconductor nanowires have been widely studied due to their unique properties such as width comparable to critical length-scales, high aspect ratio, and high carrier mobility. These unique properties make them a suitable candidate for various optical and electronic devices like photovoltaics, photodetectors, and field effect transistors. The nanowire surface plays an important role in the performance of these devices because of their high surface to volume ratio. The larger surface area of nanowires may provide better charge separation than planar heterostructures in photovoltaics by providing shorter distance to move for carrier before separation, however, presence of surface states may lead …

Development And Application Of Foraminiferal Carbonate System Proxies To Quantify Ocean Acidification In The California Current, Emily B. Osborne

Theses and Dissertations

The oceanic uptake of anthropogenic carbon has mitigated climate change, but has also resulted in a global average 0.1 decline in surface ocean pH over 20th century known as ocean acidification. The parallel reduction in carbonate ion concentration ([CO32-]) and the saturation state of seawater (Ω) has caused many major calcium carbonate-secreting organisms such as planktonic foraminifera to exhibit impaired calcification. We develop proxy calibrations and down core records that use calcification and geochemical characteristics of planktonic foraminifera as proxies for the marine carbonate system. This study focuses specifically on the surface ocean chemistry of the California Current Ecosystem (CCE), …

Assessing Individual Transit Vulnerability To Nuisance Flooding In The Charleston, Sc Area, Sumathee Selvaraj

Theses and Dissertations

Minor coastal flooding, also known as nuisance flooding, is projected to be more frequent due to relative sea level rise. Nuisance flood events in Charleston have resulted in various social impacts caused by road closures, traffic disruptions, and economic losses. This thesis presents research conducted to understand the dimensions of individual transit vulnerability to nuisance flooding and how transit vulnerability will be affected by increased extents of nuisance flooding driven by rising sea levels and heavy rainfall. Mixed methods were used to conduct this research in Berkley, Charleston, and Dorchester Counties, South Carolina. An electronic, in-person survey was administered at …

Hydro-Geological Flow Analysis Using Hidden Markov Models, Chandrahas Raj Venkat Gurram

Theses and Dissertations

Hidden Markov Models a class of statistical models used in various disciplines for understanding speech, finding different types of genes responsible for cancer and much more. In this thesis, Hidden Markov Models are used to obtain hidden states that can correlate the flow changes in the Wakulla Spring Cave. Sensors installed in the tunnels of Wakulla Spring Cave have recorded huge correlated changes in the water flows at numerous tunnels. Assuming the correlated flow changes are a consequence of system being in a set of discrete states, a Hidden Markov Model is calculated. This model comprising all the sensors installed …

Structural, Functional, And Behavioral Alterations To The Dopamine System In The Female Hiv-1 Transgenic Rat, Robert Francis Roscoe Jr.

Theses and Dissertations

HIV-associated cognitive disorders continue to affect approximately 25 million individuals worldwide, and its prevalence is expected to increase as the lifespan of HIV-1 infected individuals continues to improve. The HIV-1 transgenic rat expresses 7 of the 9 genes that encompass the virus, and is an appropriate model for studying chronic HIV-1 infection at a level that is controlled through combined antiretroviral therapy. Psychostimulant abuse is known to exacerbate HAND symptomology, but the psychostimulant methylphenidate is a first line of treatment for HIV associated cognitive inhibition. Dopamine transport abnormalities in the HIV-1 Tg rat have been well characterized through behavioral testing; …

A Mechanical Approach To The Characterization Of Material Failure Of Atherosclerotic Lesions, Lindsey A. Davis

Theses and Dissertations

Failure of atherosclerotic plaques can lead to potentially life threatening clinical events such as myocardial infarction (MI), stroke, or transient ischemic attack (TIA). The most frequently described plaque failure mechanism is tensile rupture of the fibrous cap; however, often during angioplasty another plaque failure mechanism occurs in which the atherosclerotic plaque separates from the internal elastic lamina (IEL). This study aims assess the material strength of atherosclerotic lesions using mechanical concepts.

To assess likelihood of plaque dissection at the vessel wall, adhesion strength was assessed in both mouse and human specimens using plaque delamination experiments. Measuring plaque adhesion in transgenic …

A Theoretical Study Of Polymer Based Drug Delivery Systems, Ebtisam Abdullatif Aldaais

Theses and Dissertations

Polymer nanostructured materials for drug delivery applications have witnessed tremendous progress in recent years due to their vast potential. One-end-grafted polymers can form grafted micelles with specific mechanical properties. Biological conditions can alter these properties resulting in the protection or release of drugs. Charged surfactants can also form micelles in an aqueous solution, which can also be manipulated through special conditions. On the micellar surface, self-organized polyelectrolytes can be stimulated to extend their attached ligands and thus increase the probability of binding to targeted receptors. This thesis focuses on modeling polymer-based drug delivery systems by studying the physical interactions between …

Computational Doping For Fuel Cell Material Design Based On Genetic Algorithms And Genetic Programming, Emrah Atilgan

Theses and Dissertations

Developing new materials have historically been time-consuming. Computational material discovery can search large design space to identify promising candidates for experimental verification. Recently, Density Functional Theory (DFT) based first principle calculation has been able to calculate many electrical and physical properties of materials, making them suitable for computational doping based material discovery. In material doping, given a base material, one can change its properties by substituting some elements with new ones or adding additional elements. In computational doping, we have a grid of atoms in a supercell, some of which can be substituted with dopant atoms. There are many possible …