Digital Commons Network^™

Defending Against Disparate Marine Turtle Nest Predators: Nesting Success Benefits From Eradicating Invasive Feral Swine And Caging Nests From Raccoons, Richard M. Engeman, David Addison, J. C. Griffin

United States Department of Agriculture Wildlife Services: Staff Publications

Nest predation can threaten marine turtle nesting success, and having to address dissimilar predator species complicates nest protection efforts. On Florida’s Keewaydin Island predation by raccoons Procyon lotor and invasive feral swine Sus scrofa are disparate, significant threats tomarine turtle nests. Using 6 years of nesting data (mostly for loggerhead marine turtles Caretta caretta) we examined the impacts of swine predation on nests and the benefits of swine eradication, caging nests to protect them from raccoon predation, and the effects of nest caging on swine predation. Nest predation by swine began in mid nesting season 2007, after which swine …

Evaluation Of An Avian Radar System In A Midwestern Landscape, Michael B. Gerringer, Steven L. Lima, Travis L. Devault

United States Department of Agriculture Wildlife Services: Staff Publications

Bird strikes in aviation are an increasing threat to both aircraft and human safety. Management efforts have focused largely on the immediate airport environment. Avian radar systems could potentially be useful in assessing bird strike threats at greater distances from the airport, at higher altitudes, and at night, but few studies have been conducted to assess the capabilities of avian radar systems. Thus, our goal was to assess the detection and tracking abilities of a commercially available avian radar system in an airport environment in Indiana, USA, during October 2011–March 2012. Transits by free-flying birds allowed us to assess radar …

Development Of Artificial Bait For Brown Treesnake Suppression, Bruce A. Kimball, Scott A. Sterling, Thomas W. Mcauliffe, Randal S. Stahl, Rafael A. Garcia, William C. Pitt

United States Department of Agriculture Wildlife Services: Staff Publications

The brown treesnake (Boiga irregularis) was accidentally introduced to Guam in the 1940s from the Admiralty Islands. A native of Australia, Papua New Guinea, and the Solomon Islands, the brown treesnake (BTS) continues to threaten the economy and ecology of Guam and is currently the subject of a cooperative program to control snake populations on the island and prevent its spread throughout the Pacific Rim. Delivery of toxic baits is a primary component of population suppression efforts. While many food items tested as baits for toxicant delivery provide relevant food prey cues leading to investigatory behaviors in BTS, …

Retention Time Of Chlorophacinone In Black-Tailed Prairie Dogs Informs Secondary Hazards From A Prairie Dog Rodenticide Bait, Gary W. Witmer, Nathan P. Snow, Rachael S. Moulton

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Secondary toxicity in mammals and birds that consume animals containing residues of anticoagulant rodenticides represents a persistent conflict between conservation, agriculture and environmental contamination. Chlorophacinone residues in black-tailed prairie dogs (Cynomys ludovicianus) represent a secondary exposure hazard to predatory and scavenging avian and mammalian species in the Central Plains of the United States, especially considering efforts to re-establish black-footed ferrets (Mustela nigripes). Rozol® Prairie Dog Bait (chlorophacinone 0.005%) is registered to control black-tailed prairie dogs in ten states throughout themidwestern and western United States.

RESULTS:We fed Rozol Prairie Dog Bait to captive black-tailed prairie dogs …

Fishing Diseased Abalone To Promote Yield And Conservation, Tal Ben-Horin, Kevin D. Lafferty, Gorka Bidegain, Hunter S. Lenihan

United States Geological Survey: Staff Publications

Past theoretical models suggest fishing disease-impacted stocks can reduce parasite transmission, but this is a good management strategy only when the exploitation required to reduce transmission does not overfish the stock. We applied this concept to a red abalone fishery so impacted by an infectious disease (withering syndrome) that stock densities plummeted and managers closed the fishery. In addition to the non-selective fishing strategy considered by past disease- fishing models, we modelled targeting (culling) infected individuals,which is plausible in red abalone because modern diagnostic tools can determine infection without harming landed abalone and the diagnostic cost is minor relative to …

Coherent Late-Holocene Climate-Driven Shifts In The Structure Of Three Rocky Mountain Lakes, Jeffrey R. Stone, Jasmine E. Saros, Gregory T. Pederson

United States Geological Survey: Staff Publications

Large-scale atmospheric pressure centers, such as the Aleutian and Icelandic Low, have a demonstrated relationship with physical lake characteristics in contemporary monitoring studies, but the responses to these phenomena are rarely observed in lake records. We observe coherent changes in the stratification patterns of three deep (>30 m) lakes inferred from fossil diatom assemblages as a response to shifts in the location and intensity of the Aleutian Low and compare these changes with similar long-term changes observed in the ¹⁸O record from the Yukon. Specifically, these records indicate that between 3.2 and 1.4 ka, the Aleutian Low shifted …

Supplementary Feeding And Endangered Avian Scavengers: Benefits, Caveats, And Controversies, Ainara Cortés- Avizanda, Guillermo Blanco, Travis L. Devault, Anil Markandya, Munir Z Virani, Joseph Brandt, José A Donázar

United States Department of Agriculture Wildlife Services: Staff Publications

Large avian scavengers are among the most vulnerable vertebrates, and many of their populations have declined severely in recent decades. To help mitigate this marked reduction in abundance, supplementary feeding stations (SFS; colloquially termed “vulture restaurants”) have been created worldwide, often without consideration of the scientific evidence supporting the suitability of the practice. SFS have been effective and important tools for conservation and reintroduction of avian scavengers. However, negative consequences can result from large aggregations of individual birds, disrupting intraguild processes and promoting density-dependent decreases in productivity. At the community level, SFS favor the congregation of predators (ie facultative scavengers), …

American Robins (Turdus Migratorius) And Cedar Waxwings (Bombycilla Cedrorum) Vary In Use Of Cultivated Cherry Orchards, Rachael A. Eaton, Catherine A. Lindell, Jeffrey Homan, George M. Linz, Brian A. Maurer

United States Department of Agriculture Wildlife Services: Staff Publications

Some fruit-eating bird species commonly consume cultivated fruit. Species-specific variation in diet preferences could result in varying use of orchards and impacts on the fruit-producing industry. However, species specific studies of avian orchard use are lacking, particularly throughout the fruit-growing season. Our objectives were to quantify the frequency of daily bird visits to orchards and the amount of time birds spent visiting orchards each day over the fruit-ripening season. Birds are well-documented consumers of cultivated sweet cherries (Prunus avium), which are relatively high in sugar and low in proteins and lipids. American Robins (Turdus migratorius) and …

Disease-Emergence Dynamics And Control In A Socially-Structured Wildlife Species, Kim M. Pepin, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Once a pathogen is introduced in a population, key factors governing rate of spread include contact structure, supply of susceptible individuals and pathogen life-history. We examined the interplay of these factors on emergence dynamics and efficacy of disease prevention and response. We contrasted transmission dynamics of livestock viruses with different life-histories in hypothetical populations of feral swine with different contact structures (homogenous, metapopulation, spatial and network). Persistence probability was near 0 for the FMDV-like case under a wide range of parameter values and contact structures, while persistence was probable for the CSFV-like case. There were no sets of conditions where …

A Noninvasive Method To Detect Mexican Wolves And Estimate Abundance, Antoinette J. Piaggio, Chip.A. Cariappa, Dyan J. Straughan, Melissa Neubaum, Margaret Dwire, Paul R. Krausman, Warren B. Ballard, David L. Bergman, Stewart W. Breck

United States Department of Agriculture Wildlife Services: Staff Publications

Monitoring wolf abundance is important for recovery efforts of Mexican wolves (Canis lupus baileyi) in the Blue Range Wolf Recovery Area in Arizona and New Mexico, USA. Although radiotelemetry has been a reliable method, collaring and tracking wolves in an expanding population will be prohibitively expensive and alternative methods to estimate abundance will become necessary. We applied 10 canid microsatellite loci to 235 Mexican wolf samples, 48 coyote (C. latrans) samples, and 14 domestic dog (C. lupus familiaris) samples to identify alleles that provide reliable separation of these species. We then evaluated an approach …

Ecosystem Services Provided By Avian Scavengers, Travis L. Devault, James C. Beasley, Zachary H. Olson, Marcos Moleón, Martina Carrete, Antoni Margalida, José Antonio Sánchez-Zapata

United States Department of Agriculture Wildlife Services: Staff Publications

Food webs developed under classical theoretical models often depict simplistic interactions among trophic levels linked by predation (Hairston et al. 1960). As a result, extensive research efforts have been devoted to studying predator-prey interactions, often ignoring the contribution of scavenging in food-web dynamics. However, recent advancements in food-web theory have recognized the widespread and critical role that scavenging plays in stabilizing food webs in ecosystems throughout the world, thus suggesting that previous models may have greatly underestimated the importance of scavenging in food web research ( Wilson and Wolkovich 2011; Barton et al. 2013). Such disregard for the importance of …

Evaluation Of A Food Bait Block For Potential Chemical Delivery To Black-Tailed Prairie Dogs (Cynomys Ludovicianus), Gary W. Witmer, Rachael S. Moulton, Jenna L. Swartz

United States Department of Agriculture Wildlife Services: Staff Publications

Fertility control is a potential method to control prairie dog populations in the urban/suburban environment. However, an effective, oral delivery system is needed. We tested a food bait block delivery system that could make baits available to prairie dogs over a number of days which would make this method more cost-effective than placing food bait by hand near burrows every day. Prairie dogs readily consumed the bait blocks stacked on vertical metal poles during the day. We found, however, that rabbits and mice also consumed the food bait blocks, mainly at night. Over the course of the study, the mean …

Resource Selection By Cougars: Influence Of Behavioral State And Season, Linsey W. Blake, Eric M. Gese

United States Department of Agriculture Wildlife Services: Staff Publications

An understanding of how a predator uses the landscape can assist in developing management plans. We modeled resource selection by cougars (Puma concolor) during 2 behavioral states (moving and killing) and 2 seasons (summer and winter) with respect to landscape characteristics using locations from global positioning system (GPS)-collared cougars in the Pryor Mountains, Montana and Wyoming, USA. Furthermore, we examined predation-specific resource selection at 2 scales (fine and coarse). When possible, we backtracked from cache sites to kill sites and used a fine-scale analysis to examine landscape characteristics of confirmed kills. At this fine scale, kill sites had …

Proportions Of Bird Damage In Tree Fruits Are Higher In Low-Fruit-Abundance Contexts, Catherine A. Lindell, Karen M.M. Steensma, Paul D. Curtis, Jason R. Boulanger, Juliet E. Carroll, Colleen Burrows, David P. Lusch, Nikki L. Rothwell, Shayna L. Wieferich, Heidi M. Henrichs, Deanna K. Leigh, Rachael A. Eaton, George M. Linz

United States Department of Agriculture Wildlife Services: Staff Publications

Frugivorous birds impose significant costs on tree fruit growers through direct consumption of fruit and grower efforts to manage birds.We documented factors that influenced tree fruit bird damage from 2012 through 2014 with a coordinated field study in Michigan, New York, and Washington. For sweet cherries, percent bird damage was higher in 2012 compared to 2013 and 2014, in Michigan and New York compared toWashington, and in blocks with more edges adjacent to non-sweet cherry land-cover types. These patterns appeared to be associated with fruit abundance patterns; 2012 was a particularly lowyield year for tree fruits in Michigan and New …

Identification Of Robust Microsatellite Markers For Wild Pig Fecal Dna, Elizabeth M. Kierepka, Shem D. Unger, David A. Keiter, James C. Beasley, Olin E. Rhodes Jr., Fred L. Cunningham, Antoinette J. Piaggio

United States Department of Agriculture Wildlife Services: Staff Publications

Collection of fecal samples for use in a genetic capture-mark-recapture framework has become popular as a noninvasive method of monitoring wildlife populations. A major caveat to this process, however, is that fecal samples often yield low quality DNA that is prone to genotyping errors, potentially leading to biases in population parameter estimation. Therefore, considerable care is required to identify robust genetic markers, especially in hot or humid conditions that may accelerate DNA degradation. We identified microsatellite loci in wild pig (Sus scrofa) fecal samples that were robust and informative within warm, humid ecosystems. To examine how degradation affected …

Olfactory Attractants And Parity Affect Prenatal Androgens And Territoriality Of Coyote Breeding Pairs, Christopher J. Schell, Julie K. Young, Elizabeth V. Lonsdorf, Jill M. Mateo, Rachel M. Santymire

United States Department of Agriculture Wildlife Services: Staff Publications

Hormones are fundamental mediators of personality traits intimately linked with reproductive success. Hence, alterations to endocrine factors may dramatically affect individual behavior that has subsequent fitness consequences. Yet it is unclear how hormonal or behavioral traits change with environmental stressors or over multiple reproductive opportunities, particularly for biparental fauna. To simulate an environmental stressor, we exposed captive coyote (Canis latrans) pairs to novel coyote odor attractants (i.e. commercial scent lures) midgestation to influence territorial behaviors, fecal glucocorticoid (FGMs) and fecal androgen metabolites (FAMs). In addition, we observed coyote pairs as first-time and experienced breeders to assess the influence …

Evaluation Of Boldness Assays And Associated Behavioral Measures In A Social Parrot, Monk Parakeet (Myiopsitta Monachus), Kaan Kerman, Kathryn E. Sieving, Colette S. Mary, Michael L. Avery

United States Department of Agriculture Wildlife Services: Staff Publications

Boldness reflects consistent individual differences in risk-taking behavior across various contexts. However, evaluating this basic assumption has largely been neglected in birds. In a captive monk parakeet population (Myiopsitta monachus; N = 33), we undertook an analysis of 7 measures across 3 commonly used boldness assays (i.e., novel object, emergence, and predator-exposure tests). Using principal component analysis, we derived 3 components (PCs). PC-2 loaded strongly with measures from emergence and predator-exposure tests; we interpreted it as the closest approximation of boldness. PC-1 and PC-3 described different aspects of feeding such as foraging activity and rate, respectively. Finally, we …

Winter And Summer Home Ranges Of American White Pelicans (Pelecanus Erythrorhynchos) Captured At Loafing Sites In The Southeastern United States, D. Tommy King, Justin W. Fischer, Bronson Strickland, W. David Walter, Fred L. Cunningham, Guiming Wang

United States Department of Agriculture Wildlife Services: Staff Publications

Satellite telemetry was used to investigate summer and winter home ranges for resident and migrant American White Pelicans (Pelecanus erythrorhynchos) captured in the southeastern United States between 2002 and 2007. Home range utilization distributions were calculated using 50% and 95% kernel density estimators with the plug-in bandwidth selector. Mean summer home ranges (95%) varied from 177 to 4,710 km2 and mean winter home ranges (95%) ranged from 185 to 916 km2. Mean 50% and 95% home ranges of adult American White Pelicans during summer tended to be larger than those during winter, whereas mean 50% and 95% home …

Environmental Factors Influencing The Occurrence Of Coyotes And Conflicts In Urban Areas, Sharon A. Poessel, Eric M. Gese, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

The increase of global urbanization can have effects on wildlife species, including carnivores such as coyotes (Canis latrans). As coyotes continue to settle in more urban areas, reports of human-coyote conflicts, such as attacks on humans or pets, may also increase. Understanding environmental variables that might influence whether or not coyotes and human-coyote conflicts will occur in certain urban areas may assist wildlife officials in creating management plans for urban wildlife.We conducted a survey of 105 urban areas in the United States requesting information on the occurrence of coyotes and human-coyote conflicts. We analyzed the responses with data …

Speed Kills: Effects Of Vehicle Speed On Avian Escape Behavior, Travis L. Devault, Bradley F. Blackwell, Thomas W. Seamans, Steven L. Lima, Esteban Fernández-Juricic

United States Department of Agriculture Wildlife Services: Staff Publications

The avoidance of vehicles is a common challenge for birds in the modern world. Birds generally rely on antipredator behaviors to avoid vehicles, but modern vehicles are faster than predators. We predicted that birds may be unable to accurately estimate the speed of approaching vehicles, which could contribute to miscalculations in avoidance behaviors and cause collisions. We tested our prediction in two studies. In the first (DeVault et al. 2014), we baited turkey vultures (Cathartes aura) to roads with animal carcasses and measured flight initiation distance (FID) when driving a truck towards them at 30, 60, or 90 …

Morphometric Variation During Chick Development In Interior Double-Crested Cormorants (Phalacrocorax Auritus), Jennifer E. Chastant, Corey T. Callaghan, D. Tommy King

United States Department of Agriculture Wildlife Services: Staff Publications

In numerous avian species, egg size is correlated to female body condition, hatchling size and nestling growth. Recent demography studies of Interior Double-crested Cormorants (Phalacrocorax auritus) suggest a migratory divide across the Great Lakes; western populations winter in the Gulf of Mexico region of the southeastern United States (Alabama, Arkansas, Louisiana, and Mississippi) with extensive catfish (Ictalurus punctatus) aquaculture, and eastern populations winter in Florida, where catfish aquaculture is not pervasive. If Double-crested Cormorants have improved their overall body condition through catfish exploitation, then egg and chick sizes should also be affected. Three breeding areas in Ontario (east, central, and …

Role For Migratory Wild Birds In The Global Spread Of Avian Influenza H5n8

United States Department of Agriculture Wildlife Services: Staff Publications

Avian influenza viruses affect both poultry production and public health. A subtype H5N8 (clade 2.3.4.4) virus, following an outbreak in poultry in South Korea in January 2014, rapidly spread worldwide in 2014–2015. Our analysis of H5N8 viral sequences, epidemiological investigations, waterfowl migration, and poultry trade showed that long-distance migratory birds can play a major role in the global spread of avian influenza viruses. Further, we found that the hemagglutinin of clade 2.3.4.4 virus was remarkably promiscuous, creating reassortants with multiple neuraminidase subtypes. Improving our understanding of the circumpolar circulation of avian influenza viruses in migratory waterfowl will help to provide …

Prioritizing Management And Research Actions Against Invasive Reptiles In Florida: A Collaboration By An Expert Panel, Richard M. Engeman, Michael L. Avery

United States Department of Agriculture Wildlife Services: Staff Publications

Florida has more introduced animals than any other region of the U.S. and also ranks high in this respect globally. Given Florida's climate, it is no coincidence that a large proportion of Florida’s invasive vertebrate species are reptiles and amphibians. Exotic snakes, lizards, frogs, turtles, and crocodilians are all breeding in Florida. The largest snakes in Florida are constrictors from other continents, and the five largest lizard species breeding in Florida are from Africa, South America, and Central America. Establishment of non-native reptiles and amphibians has been documented in Florida for over 135 years, and the rate of invasive reptile …

Antigenic Characterization Of H3 Subtypes Of Avian Influenza A Viruses From North America, Elizabeth Bailey, Li-Ping Long, Nan Zhao, Jeffrey S. Hall, John A. Baroch, Jacqueline M. Nolting, Lucy Senter, Frederick L. Cunningham, G. Todd Pharr, Larry Hanson, Richard Slemons, Thomas J. Deliberto, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Besides humans, H3 subtypes of influenza A viruses (IAVs) can infect various animal hosts, including avian, swine, equine, canine, and sea mammal species. These H3 viruses are both antigenically and genetically diverse. Here, we characterized the antigenic diversity of contemporary H3 avian IAVs recovered from migratory birds in North America. Hemagglutination inhibition (HI) assays were performed on 37 H3 isolates of avian IAVs recovered from 2007 to 2011 using generated reference chicken sera. These isolates were recovered from samples taken in the Atlantic, Mississippi, Central, and Pacific waterfowl migration flyways. Antisera to all the tested H3 isolates cross-reacted with each …

Weak Support For Disappearance And Restricted Emergence/Persistence Of Highly Pathogenic Influenza A In North American Waterfowl, Andrew M. Ramey, Erica Spackman, Mia Kim-Torchetti, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

Krauss et al. (1) use lack of detection of highly pathogenic (HP) H5 clade 2.3.4.4 (henceforth “H5”) influenza A viruses (IAVs) from >22,000 wild bird samples collected in North America in 2014–2015 to argue that HP H5 IAVs disappeared from waterfowl and that unresolved mechanisms restrict emergence and perpetuation of HP IAVs in natural reservoir species. Here we offer an alternative interpretation.

Use Of Predator Hair To Enhance Perceived Risk To White-Tailed Deer In A Foraging Context, Thomas W. Seamans, Bradley F. Blackwell, Kimberly E. Linnell

United States Department of Agriculture Wildlife Services: Staff Publications

Deer react to predator scent in varying degrees even when exposed to unknown predators. This response could be genetically based and maintained as long as the population is exposed to predation. We tested whether predator scent in the form of hair would enhance perceived risk and serve as a foraging repellent to free-ranging white-tailed deer (Odocoileus virginianus). During the winters of 2013 and 2014, we quantifi ed alert behaviors and consumption of whole-kernel corn in response to current (coyote [Canis latrans]) and extirpated (bobcat [Lynx rufus]; black bear [Ursus americanus]) predator species …

Repellent Application Strategy For Wild Rodents And Cottontail Rabbits, Scott J. Werner, Shelagh T. Deliberto, Roger A. Baldwin, Gary W. Witmer

United States Department of Agriculture Wildlife Services: Staff Publications

Effective chemical repellents and repellent application strategies are needed to manage damages caused by wild rodents and rabbits to agricultural resources. For the purpose of comparatively investigating the behavioral response of wild rodents and rabbits to a chemical repellent, we experimentally evaluated the concentration-response relationship of an anthraquinone-based repellent in California voles (Microtus californicus Peale), Richardson’s ground squirrels (Urocitellus richardsonii Sabine), deer mice (Peromyscus maniculatus Wagner) and cottontail rabbits (Sylvilagus audubonii Baird) in captivity. We observed 52–56% feeding repellency for whole oats treated with 10,800ppm anthraquinone or 18,500ppmanthraquinone in mice and squirrels, and 84–85% repellency for …

Multi-Isotopic (Δ2H, Δ13C, Δ15N) Tracing Of Molt Origin For Red-Winged Blackbirds Associated With Agro-Ecosystems, Scott J. Werner, Keith A. Hobson, Steven L. Van Wilgenburg, Justin W. Fischer

United States Department of Agriculture Wildlife Services: Staff Publications

We analyzed stable-hydrogen (δ²H), carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope ratios in feathers to better understand the molt origin and food habits of Red-winged Blackbirds (Agelaius phoeniceus) near sunflower production in the Upper Midwest and rice production in the Mid-South of the United States. Outer primary feathers were used from 661 after-second-year (ASY) male blackbirds collected in Minnesota, Montana, North Dakota and South Dakota (spring collection), and Arkansas, Louisiana, Mississippi, Missouri and Texas (winter collection). The best-fit model indicated that the combination of feather δ²H, δ¹³C and δ …

Immunotherapy Using Algal-Produced Ara H 1 Core Domain Suppresses Peanut Allergy In Mice, James A. Grgeory, Ariel Shepley-Mctaggart, Michelle Umpierrez, Barry K. Hurlburt, Soheila J. Maleki, Hugh A. Sampson, Stephen P. Mayfield, M. Cecilia Berin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates immunotherapy studies. Moreover, peanut immunotherapy is associated with frequent negative side effects and patients are often at risk of allergic reactions once immunotherapy is discontinued. Allergen-specific approaches using recombinant proteins are an attractive alternative because they allow more precise dosing and the opportunity to engineer proteins with improved safety profiles. We tested whether Ara h …

Component Greenhouse Gas Fluxes And Radiative Balance From Two Deltaic Marshes In Louisiana: Pairing Chamber Techniques And Eddy Covariance, Ken W. Krauss, Guerry O. Holm Jr., Brian C. Perez, David E. Mcwhorter, Nicole Cormier, Rebecca F. Moss, Darren J. Johnson, Scott C. Neubauer, Richard C. Raynie

United States Geological Survey: Staff Publications

Coastal marshes take up atmospheric CO₂ while emitting CO₂, CH₄, and N₂O. This ability to sequester carbon (C) is much greater for wetlands on a per area basis than from most ecosystems, facilitating scientific, political, and economic interest in their value as greenhouse gas sinks. However, the greenhouse gas balance of Gulf of Mexico wetlands is particularly understudied. We describe the net ecosystem exchange (NEE_c) of CO₂ and CH₄ using eddy covariance (EC) in comparison with fluxes of CO₂, CH₄, and N₂O using …