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Characterization Of An Al$^{3+}$-Selective Fluorescent Probe Based On A Benzoyl Hydrazine Derivative And Its Application In Cell Imaging, Yuxiang Ji, Chunwei Yu, Shaobai Wen, Jun Zhang

Turkish Journal of Chemistry

A fluorescent probe based on benzoyl hydrazine was synthesized and characterized as an Al$^{3+}$-selective fluorescent probe. This probe showed good selectivity towards Al$^{3+\, }$compared to other common ions. Under optimized experimental conditions, the probe exhibited a linear dynamic response for Al$^{3+}$ from 5.0 $\times $ 10$^{-7}$ to 4.5 $\times $ 10$^{-6}$ M with a detection limit of 1.3 $\times $ 10$^{-7}$ M in ethanol-water solution (9:1, v:v, pH 6.8, 20 mM HEPES). Furthermore, it was used for imaging of Al$^{3+}$ in living cells with satisfying results.

Remediation Of Cr(Vi) Via Combined Self-Reduction And Adsorption Bychemically Modified Carbon Sorbents, Mohamed E. Mahmoud, Amr. A. Yakout, Asmaa M. Halbas, Maher M. Osman

Turkish Journal of Chemistry

Hexavalent chromium, Cr(VI), is a highly toxic species, while trivalent chromium, Cr(III), is an essential trace element for humans and other organisms. In the present work, a simple, rapid, and efficient procedure for water treatment of Cr(VI) is described. Active carbon (AC) was chemically treated with HCl and NaOH for the formation of surface modified Acidic-AC and Basic-AC sorbents, respectively. In addition, AC sorbent was treated with HNO$_{3}$ as an oxidizing agent and sodium sulfite as a reducing agent for the formation of Ox-AC and Rd-AC sorbents, respectively. The modified AC sorbents were tested to identify their potential applications in …

Nathaniel Gist Gee Papers - Accession 395, Nathaniel Gist Gee

Manuscript Collection

The Nathaniel Gist Gee Papers consist of various papers concerning Gee’s years in China including correspondence, reports, newspaper and magazine clippings, Chinese poetry, statistics, manifestos, statements, his professional papers concerned primarily with freshwater sponges, and professional papers and publications he collected that chronicle China’s growth. Many of the records are concerned with China’s political and social changes including China’s Nationalist and Student Movements during Gee’s stay in China.

Nathaniel Gist Gee (1876-1937), born in Union, SC, was a professor of Natural Science at Soochow University and Yenching University in China from 1901-1932.

Evaluation Of Apoptotic Cell Death Mechanisms Induced By Hypericin-Mediated Photodynamic Therapy In Colon Cancer Cells, Aysun Kiliç Süloğlu, Eli̇f Karacaoğlu, Güldeni̇z Selmanoğlu, Hayri̇ye Akel, İbrahi̇m Çağatay Karaaslan

Turkish Journal of Biology

Hypericin (HYP)-mediated photodynamic therapy (PDT) is a new alternative treatment strategy for colon cancer inducing various cell-death pathways. Apoptotic cell death is the desired cellular fate in cancer cells. Therefore, we investigated the apoptotic pathways by determining apoptosis-related proteins (survivin, caspase-3, caspase-9, Bcl-2, and Bax) at the mRNA level using real-time polymerase chain reaction (qPCR), and the percentage of survivin was determined by survivin ELISA in HT-29 and Caco-2 colon cancer cell lines. The downregulation of survivin was significant 16 h after PDT for both cells, while caspase-3 upregulation was apparent 24 h after PDT. Caspase-9 and caspase-3 upregulations were …

Human Embryonic Stem Cell N-Glycan Features Relevant To Pluripotency, Sabi̇re Karaçali

Turkish Journal of Biology

Human embryonic stem cells (hESCs) and reprogrammed human induced pluripotent stem cells (hiPSCs) proliferate indefinitely in the undifferentiated state (self-renewal) and differentiate into the three germ layers (and ultimately into all cell types) (pluripotency). Branching in the N-glycan core of hESCs/hiPSCs is limited. hESCs/hiPSCs have high mannose-type and biantennary complex-type core structures, indicating immature stages of N-glycoproteins. The two branches on the core consist of type 2 N-acetyllactosamine (LacNAc), completed mainly with α 2,6-linked sialic acid. Complex terminal fucosylation, particularly α 1,2-linked fucosylation, is another characteristic N-glycosylation feature of hESCs/hiPSCs. All of these structural features are probably associated with the …

Effects Of Scattering And Absorption On Laser Speckle Contrast Imaging, Kosar Khaksari

Dissertations, Master's Theses and Master's Reports

Laser Speckle Contrast Imaging (LSCI) is a real-time, non-invasive method in used to investigate blood flow and perfusion in biological tissues with high temporal and spatial resolution. A reduction in speckle contrast due to particle motion is the primary contrast mechanism in LSCI. Motion results in speckle fluctuations in time and reduces the contrast over a given camera integration period. There are a variety of parameters that effect contrast besides motion. The optical properties of the scattering medium are one of the parameters effecting LSCI values. Changes in blood hematocrit levels manifest as changes in optical properties. In this work, …

Hardware Design And Integration And Software Programming For Optical Elastography, Nima Taherkhani

Dissertations, Master's Theses and Master's Reports

The elastic and viscoelastic properties of skin are a reflection of its health. Measurement of these elastic properties can be used as the indicator of various pathologies. The measurement concept in our works is to mechanically stimulate the skin with a low frequency pneumatic force using non-invasive means of deflecting the skin and use laser speckle to study the elasticity of the skin.

The goal of this work was to develop a setup for in vivo optical elastography of skin. This setup includes an electro-mechanical device which is able to deliver a sinusoidal pneumatic stimulus equivalent to 10 grams of …

The Utility Of The Participatory Approach For Sustainable Development Assessments, Ashma Vaidya

Dissertations, Master's Theses and Master's Reports

‘Sustainability’ may be a generalizable concept; its definition however, heavily depends upon context. Understanding the context (sociocultural, environmental, socioeconomic, political etc.) is crucial for defining and assessing the sustainability of any given socio-ecological system. This point underlies our research design and objectives. The primary objectives of this research were to understand the sustainability context in the Upper Peninsula of Michigan, and to use it to develop a sustainability assessment framework for a potential forest-based bioenergy industry in the region. We first reviewed top-down and bottom-up sustainability assessment frameworks. Then we used the best-suited approach i.e. bottom-up participatory approach, to pursue …

Information Technology And Computer Science Education At St. Patrick’S College In The Republic Of Vanuatu, Timothy R. Ward

Dissertations, Master's Theses and Master's Reports

This report examines the role of an Information Technology and Computer Science Education Volunteer with the United States Peace Corps in the Republic of Vanuatu at St. Patrick’s College. Various methods of developing curriculum, training teachers, and teaching classes were used and the results are analyzed. Primarily cultural and logistical challenges and their associated newly discovered approaches are discussed. Some approaches were found to improve Information Technology and Computer Science Education at St. Patrick’s College.

Wireless Implantable Magnetoelastic Sensors And Actuators For Biomedical Applications, Andrew Derouin

Dissertations, Master's Theses and Master's Reports

Magnetoelastic sensors represent a low-cost wireless and battery-less method for monitoring parameters in embedded or implanted applications; however, some limitations still exist preventing their commercial implementation. Presented in this work are a variety of studies that are aimed at improving the feasibility of magnetoelastic materials for sensing and actuating applications. Magnetoelastic resonant sensors of non-standard geometries were investigated to determine if geometry could play a role on the sensitivity of the sensor response to mass loading. It was shown that a significant increase in sensitivity could be achieved by using triangular sensors rather than standard rectangular strips. A method for …

A Wireless Sensor System With Digitally Controlled Signal Conditioning Circuit For Force Monitoring At Bone Fixation Plates, Govindan Suresh

Dissertations, Master's Theses and Master's Reports

Post-rehabilitation of orthopedic surgery is critical for bone fracture treatments. Current protocols are not based on quantitative assessments of the patient condition but they are conservative estimations mostly based on prior experience and physician’s opinions. While there are quantitative methods for assessing the recovery of orthopedic surgery, they are typically very expensive and provide only snapshots during the healing process. A standalone, reconfigurable, embedded wireless sensor system with digitally controlled signal conditioning system capable of providing continuous monitoring of bone healing is developed. Strain sensor measurements were validated against a commercial mechanical loading instrument for relevant loads that an animal …

Salt Sensitive Hypertension And Orexin, Mike Huber

Dissertations, Master's Theses and Master's Reports

Elevated plasma arginine vasopressin (AVP) levels have been found in human hypertension subjects and several salt dependent experimental animal models of hypertension including the Dahl salt sensitive hypertension (SSHTN) model. The orexin system is involved in AVP regulation and its over activation has been implicated in hypertension, however, the role of brain orexin in SSHTN is unknown. We hypothesized that increased activity of orexin in the paraventricular nucleus (PVN), a prominent region in AVP production, contributes to SSHTN via enhancing AVP signaling. Eight-week-old male adult Dahl salt sensitive (DS) and age and sex matched Sprague Dawley (SD) rats were placed …

Objective Bayesian Analysis Of A Generalized Lognormal Distribution, Shengnan Li

Dissertations, Master's Theses and Master's Reports

The generalized lognormal distribution plays an important role in various aspects of life testing experiments. We examine Bayesian analysis of this distribution using objective priors (in the general sense of priors constructed using some formal rules) for the model parameters in this paper. Specifically, the derivation of explicit expressions for multiple types of the Jeffreys priors, the reference priors with different group ordering of the parameters, and the first-order matching priors. We investigate the important issue of proper posterior distributions. It is shown that only two of them lead to proper posterior distributions. Monte Carlo simulations are conducted to compare …

Small Size Coupling Feed And Inductive Shorting Antenna For Wide Bandwidth, Increased Gain And Efficiency With Low Specific Absorption Rate (Sar) Operation, Md Mahfuzur Rahman

Dissertations, Master's Theses and Master's Reports

Day by day technology is changing our lives rapidly. With these radical changes, demands in communication industry are also increasing drastically. To cope up with these demands, 2G, 3G, 4G, LTE (Long Term Evolution) communication systems have been introduced. Modern cellular phone should be capable to operate in wide range of frequency spectrum for their global access to provide end user customers these communication services. Hence mobile antennas should be designed in such a way that they should generate wideband operation. But commercially available standalone mobile antennas aren’t capable to operate in all those communication systems, several antennas are used …

Carbon Flux Dynamics In High Altitude Peatlands In The Ecuadorian Andes, Maria Elisa Sanchez Garces

Dissertations, Master's Theses and Master's Reports

Although knowledge of peatland CH₄ and CO₂ exchange in temperate mountain ecosystems is available, information about carbon (C) exchange in peatlands of the Andean mountains is limited and these ecosystems may behave differently given the particular characteristics of the Andean tropics. These ecosystems are highly productive and under pressure by grazing. Our first objective was to measure baseline carbon dioxide (CO₂) and methane (CH₄) flux in an undisturbed peatland in Cayambe-Coca National Park. Our second objective was to quantify CO₂ and CH₄ fluxes in an intensively cattle grazed peatland near Antisana Ecological …

Geoheritage Of The Keweenaw Peninsula, Erika Vye

Dissertations, Master's Theses and Master's Reports

The Keweenaw Peninsula is a compelling intersection of cultural, industrial, and mining heritage - all of which is rooted in its ancient geologic underpinnings. The incredible geodiversity of the Keweenaw expresses a billion year geologic history that has shaped the landscape and resulted in the discovery of one of the largest native copper deposits on Earth; in turn, originating one of the oldest metal workings in the Western Hemisphere, and the famed mining boom of the Keweenaw in the late 1800’s. This rich human story intertwined with globally significant geosites has endowed the Keweenaw with a strong geoheritage.

Geoheritage considers …

First-Principles Studies Of Group Iv And Group V Related Two Dimensional Materials, Gaoxue Wang

Dissertations, Master's Theses and Master's Reports

Two dimensional (2D) materials have been extensively studied due to their novel properties and technologically important applications. Especially, the discovery of graphene has stimulated an avalanche of investigations to exploit its novel properties for applications at nanoscale. In the post-silicon era, graphene has been widely regarded as the most promising building blocks for the electronic devices. However, its metallic nature together with sensitivity to the environment leads to somewhat limited scope of applications. A finite band gap in a material is known to be essential for the fabrication of devices such as transistors. Such a limitation associated with graphene has …

An Examination Of Autism Symptomatology In Young Children With Family History Of Autism Spectrum Disorder, Jasper Abarte Estabillo

LSU Master's Theses

Multiple genetic and environmental factors have roles in the etiology of autism spectrum disorder (ASD). Thus, researchers have become increasingly interested in studying family members of individuals with ASD in order to examine possible risk factors and to identify early markers of the disorder. While family history of ASD may put an individual at risk for developing autism, there is limited research examining how the degree of relationship to the affected individual may be related to an individual’s presenting ASD symptomatology. Because closer familial relationships (i.e., first-degree relatives) have more shared genetic material and tend to have increased common environment …

A Geochronological And Stratigraphic Reconstruction Of The Middle Barataria Bay Receiving Basin, Joseph Ethan Thomas Hughes

LSU Master's Theses

Barataria Bay, one of the largest receiving basins for the Mississippi deltaic complex, is the location of a proposed river-sediment diversion for delta restoration. In order to determine how the sediment in the receiving-basin may respond to diversion flows, twenty-five sediment vibracores were collected from a 115 km2 study area located near Myrtle Grove and Bayou Dupont, southeast of New Orleans, LA. These cores were subject to multiple tests, including gamma bulk density scans, grain size analysis, and loss-on-ignition, in order to identify the lithology and stratigraphy. In addition, 137Cs and 14C dating techniques were employed in order to construct …

A Model Compound Study In Syngas Tar Reforming, Jaren Lee

LSU Master's Theses

Biomass or coal gasification are promising processes for renewable energy. However, a major operating problem in gasification is what to do with syngas byproducts such as H2S and tars (heavy hydrocarbons) that cause catalyst deactivation downstream. Studies have shown that rare earth oxides (REOs) doped with transition metals are promising catalysts for tar reforming in the presence of sulfur.(R. Li, Roy, Bridges, & Dooley, 2014) In Chapter 1, propane is studied as a model compound for tar reforming with transition metal-doped (e.g., Mn, Fe) REOs. These are compared to a typical commercial Ni/Al2O3 catalyst. The results showed that REO/TM catalysts …

Activity Level As A Moderator Of The Relationship Between Child And Maternal Anxiety, Paige Marie Ryan

LSU Master's Theses

Anxiety is excessive and impairing for approximately 30% of individuals. Families with anxiety problems tend to pass on those problems to their children, specifically due to a variety of factors: genetics, parenting style, and other environmental risks. On the other hand, promoting behaviors like positive activity scheduling, participation in clubs, and physical activity has been shown to decrease symptoms of anxiety. However, research has yet to focus on this relationship by increasing activity level (e.g., participation in organizations, hobbies, sports). The purpose of this study is to investigate if the relationship between maternal and child anxiety is dependent upon the …

Influence Of Fertilizer Source And Irrigation Regimen On Containerized Production Of Coleus (Plectranthus Scutellarioides (L.) Codd) 'Solar Sunrise' And Management Of Bermudagrass (Cynodon Dactylon (L.) Pers. X C. Transvaalensis Burtt-Davy) 'Tifway', Kayla Renee Sanders

LSU Master's Theses

Fertilizer nutrient losses through leachate and runoff from excessive irrigation in nursery container production and turfgrass management can be high and have negative environmental impacts. The objective of this research was to examine the influence of fertilizer source and irrigation regimen on nutrient losses during nursery container production and turfgrass management. During the container production of coleus (Plectranthus scutellarioides (L.) Codd) 'Solar Sunrise', four fertilizer treatments: an unfertilized control; a controlled-release (CRF); a water-soluble (WSF); and a combination of 10% WSF and 90% CRF, were incorporated into a pine bark substrate at 0.30 kg N and P·m-3 in 3.7-L containers …

Arbuscular Mycorrhizal Fungi Of Northern White Cedar (Thuja Occidentalis L.): Habitat Effects On Fungal Communities And Inoculum Effects On Plant Growth On Acid Peat Soils, Guswarni Anwar

Dissertations, Master's Theses and Master's Reports

The relationship of arbuscular mycorrhizal (AM) fungi with northern white cedar (NWC) was examined from the perspective of both fundamental questions about habitat specificity in the root fungal community, as well as applied questions regarding AM fungal efficacy in NWC restoration in peat soils. I performed two experiments testing the effects of AM fungi on survival, growth, and nutrition of NWC seedlings; and one molecular study to determine the habitat effects on community composition of NWC root-associated fungi. First, a greenhouse AM inoculation experiment was conducted in factorial combination with fertilization and liming to examine conditional effectiveness of AM fungal …

Technical Solar Photovoltaic Potential Of Large Scale Parking Lot Canopies, Ram Krishnan

Dissertations, Master's Theses and Master's Reports

Solar photovoltaic (PV) technology can provide sustainable power for the growing global population, but it demands considerable land area. This is a challenge for densely populated cities.

However, the stranded assets of non-productive parking lots areas can be converted to solar farms with PV canopies, enabling sustainable electricity generation while preserving their function to park automobiles.

This report provides a method for determining the technical and economic potential for converting a national scale retail company's parking lot area to a solar farm. First, the parking lot area for the company is determined and divided into zones based upon solar flux …

Modeling The Physical And Biogeochemical Processes In Lake Superior Using Lake2k, Sheelagh M. Mccarthy

Dissertations, Master's Theses and Master's Reports

Modeling lake processes and dynamics improves understanding of the system and supports predictions of the response of the lake to perturbations, such as climate change. LAKE2K, a 3-layer surface water quality model, uses a mass balance approach to simulate the physical and biogeochemical cycles in Lake Superior. The model is successfully calibrated with data from offshore Lake Superior in 2011, a year with average meteorological conditions. The thermal regime, phytoplankton populations, and phosphorus cycle of Lake Superior are modeled, resulting in a representation of seasonal trends in this dimictic system. The calibrated model is confirmed with an application for Lake …

Laboratory Measurements Of Contact Nucleation By Mineral Dusts, Bacteria, And Soluble Salts, Joseph Niehaus

Dissertations, Master's Theses and Master's Reports

We present results from experiments that emulate atmospheric ice nucleation by aerosols. We have refined experimental techniques to improve measurements of ice forming nuclei in the contact mode. Our results show that atmospherically relevant dusts such as kaolinite, feldspar, rhyolitic ash, and Arizona Test Dust have efficiencies in the contact mode that are higher than the immersion mode. Experiments with bacteria show that biological material has the potential to contribute significantly to ice concentrations, but has large variability. By choosing a soluble compound as an ice nucleus, we are able to place bounds on the timescale for contact freezing and …

The Effect Of Posttranslational Modifications On Protein Aggregation, Morphology, And Toxicity, Mu Yang

Dissertations, Master's Theses and Master's Reports

Proteins are one of the most versatile macromolecules in the biological system. The function or activity of a protein highly depends on its 3D native structure. However, under stress, they are at risk of misfolding/aggregation, leading to formation of structures that can indicate loss of function or gain of toxicity. In severe cases, protein aggregation can result in many diseases, including neurodegenerative diseases, such as Alzheimer’s, Parkinson’s, Huntington’s, and amyotrophic lateral sclerosis. Due to the heterogeneous nature of cellular environment and protein molecules, mechanism of in vivo folding and related toxicity still remains elusive. To have a better understanding of …

A Characterization Of The Water Quality Conditions And Pollutant Loads In Surface Waters Near The Northeast Ohio Regional Sewer District’S Combined Sewer System In Cleveland, Ohio, Nathan Zgnilec

Dissertations, Master's Theses and Master's Reports

The Northeast Ohio Regional Sewer District (NEORSD, Cleveland, OH) serves over one million citizens through the operation of three waste water treatment plants. NEORSD provides this service through combined and separated sewer systems, which, during rainfall events, are known to deliver large volumes of untreated wastewater containing high concentrations of Escherichia coli (E.coli), ammonia nitrogen, and phosphorus. NEORSD is currently investing $3 billion over the next 25 years to mitigate its combined sewer overflows and plans to allocate additional funds to improve remaining pollutant sources related to its system, e.g. separated sewers. A suite of PC-SWMM pollutant loading models have …

Effect Of Disulfide Bond Scrambling On Protein Stability, Aggregation, And Cytotoxicity, Colina Dutta

Dissertations, Master's Theses and Master's Reports

Proteins are nano-machines that carry out majority of the cellular functions. Thermodynamically they are functional and stable within a very narrow range (1 kcal/mol). External perturbations in the form of pH change, thermal, or oxidative/reducing stress can destabilize the protein resulting in misfolding and aggregation. Prolonged environmental stress can affect the cells adaptive response resulting in loss of ability to refold or recycle proteins. This can lead to accumulation of misfolded or aggregated proteins within the cell. Such accumulation of aggregated proteins have been associated with neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS), Parkinson’s, Huntington’s, and Alzheimer’s disease. There …

Inhibition Of Bacterial Growth And Prevention Of Bacterial Adhesion With Localized Nitric Oxide Delivery, Julia Osborne

Dissertations, Master's Theses and Master's Reports

Bacterial infections continue to be a problem at the site of an indwelling medical device, and over the years, various bacterial strains have become more resistant to current antibiotic treatments. Bacterial infection at an indwelling medical device can be dangerous and affect the performance of the medical device which can ultimately lead to the failure of the device due to bacterial resistance to treatment.

Nitric Oxide (NO) has been shown to possess antibacterial properties to prevent and inhibit bacterial growth. NO releasing coatings on indwelling medical devices could provide a reduction in bacterial infections that occur at the device site …