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Modulating Male Aggression And Courtship: Detecting External Pheromonal And Nutritional Information, Jonathan C. Andrews

Graduate Student Theses, Dissertations, & Professional Papers

Survival and reproduction in the natural world requires an organism to identify and react to the presence of environmental stimuli in a time and cue dependent manner. Such temporal specificity requires the development and use of specialized sensory organs that receive this external sensory information. Neurons within the specialized sensory organs respond to touch, taste, pheromones, chemicals, and light, and transduce this information to the central brain. In many systems, gustatory and olfactory chemosensation in particular, provides critical information regarding sex and species identification as well as the status of food resources. The output of neurons which receive chemical information …

Honors @ Georgia Southern, University Honors Program Students And Staff, Georgia Southern University

Honors College: News & Publications (2008-2020)

• Honors Alumnae Published
• Honors LLC
• Honors in Action
• Alumni News

Electrosensitization Assists Cell Ablation By Nanosecond Pulsed Electric Field In 3d Cultures, Claudia Muratori, Andrei G. Pakhomov, Shu Xiao, Olga N. Pakhomova

Bioelectrics Publications

Previous studies reported a delayed increase of sensitivity to electroporation (termed "electrosensitization") in mammalian cells that had been subjected to electroporation. Electrosensitization facilitated membrane permeabilization and reduced survival in cell suspensions when the electric pulse treatments were split in fractions. The present study was aimed to visualize the effect of sensitization and establish its utility for cell ablation. We used KLN 205 squamous carcinoma cells embedded in an agarose gel and cell spheroids in Matrigel. A local ablation was created by a train of 200 to 600 of 300-ns pulses (50 Hz, 300-600 V) delivered by a two-needle probe with …

Thermal Assisted In Vivo Gene Electrotransfer, Amy Donate, Anna Bulysheva, Chelsea Edelblute, Derrick Jung, A. Malik, Siqi Quo, Niculina Burcus, Karl Schoenbach, Richard Heller

Bioelectrics Publications

Gene electrotransfer is an effective approach for delivering plasmid DNA to a variety of tissues. Delivery of molecules with electric pulses requires control of the electrical parameters to achieve effective delivery. Since discomfort or tissue damage may occur with high applied voltage, the reduction of the applied voltage while achieving the desired expression may be an important improvement. One possible approach is to combine electrotransfer with exogenously applied heat. Previous work performed in vitro demonstrated that increasing temperature before pulsing can enhance gene expression and made it possible to reduce electric fields while maintaining expression levels. In the study reported …

Inactivation Of Acanthamoeba Spp. And Other Ocular Pathogens By Application Of Cold Atmospheric Gas Plasma, Wayne Heaselgrave, Gilbert Shama, Peter W. Andrew, Michael G. Kong

Bioelectrics Publications

Currently there are estimated to be approximately 3.7 million contact lens wearers in the United Kingdom and 39.2 million in North America. Contact lens wear is a major risk factor for developing an infection of the cornea known as keratitis due to poor lens hygiene practices. While there is an international standard for testing disinfection methods against bacteria and fungi (ISO 14729), no such guidelines exist for the protozoan Acanthamoeba, which causes a potentially blinding keratitis most commonly seen in contact lens wearers, and as a result, many commercially available disinfecting solutions show incomplete disinfection after 6 and 24 …

A Dielectric Rod Antenna For Picosecond Pulse Stimulation Of Neurological Tissue, Ross A. Petrella, Karl H. Schoenbach, Shu Xiao

Bioelectrics Publications

A dielectrically loaded wideband rod antenna has been studied as a pulse delivery system to subcutaneous tissues. Simulation results applying 100-ps electrical pulse show that it allows us to generate a critical electric field for biological effects, such as brain stimulation, in the range of several centimeters. In order to reach the critical electric field for biological effects, which is similar to 20 kV/cm, at a depth of 2 cm, the input voltage needs to be 175 kV. The electric field spot size in the brain at this position is similar to 1 cm(2). Experimental studies in free space with …

Csbs Statements, V18, 2016, University Of Northern Iowa. College Of Social And Behavioral Sciences.

CSBS Statements

Contents:

--From the Desk of the Dean
--Where in the World is CSBS
--Fashion & STEM: A Perfect Fit
--Sociology of Culture: Engaging New Tastes Thru Art, Chocolate and Community
--Passing the Torch for a Brighter Future
--UNI ROTC Celebrates Shared Anniversary With Army ROTC
--History and Military Science Team Up to Teach Future Citizens
--ROTC Cadet Summer Training
--Social Work Explores Seoul Exchange Program
--The Canterbury Forum
--Elect Her Panther Women Win
--State-of-the-art Lab Gives UNI Students an Opportunityto Impact the Field
--Iowa Bioblitz Program Inspires Young Explorers
--Geotree Center Offers Students Real-World Experience
--Out of the Darkness Walk …

Exploring The Link Between Foliar Water Uptake Capacity And Climate In Coast Redwood, Yvette M. Ferreira

Cal Poly Humboldt theses and projects

Coast redwood (Sequoia sempervirens D. Don) depends on foliar water uptake from fog and rain to maintain water balance during the dry summer season. As fog and rain decline across the redwood region, understanding how foliar uptake capacity responds to climate has become increasingly important. This study investigated how three peripheral leaf traits that influence foliar water uptake, visible wax coverage, stomatal density, and guard cell length, vary across climatic gradients and how those traits affect uptake across 14 sites in California. Foliar water uptake was quantified using rain-chamber rehydration experiments, climatic predictors were evaluated using least absolute shrinkage …

Src Family Kinases Mediate Individual Cell Responses To Receptor Tyrosine Kinase Signaling In Neuroblastoma Cells, Juan Enrique Palacios Moreno

Graduate Student Theses, Dissertations, & Professional Papers

Receptor Tyrosine Kinases (RTKs) are involved in proliferation, differentiation, cellular metabolism, and homeostasis. More than half of the RTKs in the human genome are expressed in neuroblastoma cell lines. RKTs activate downstream signaling pathways including the canonical pathways of ERK, AKT, PLCγ, and SRC Family Kinases (SFKs). Phosphoproteomic data showed high expression and phosphorylation levels of SFK scaffold PAG1, and SFKs FYN and LYN in neuroblastoma cell lines and endosomes. We hypothesized that FYN and LYN distinguish between proliferation- and differentiation-inducing signals from different RTKs in neuroblastoma cells.

To test this hypothesis, we measured changes in FYN and LYN’s intracellular …

Empathy And The Atonement, Tyler Johnson

BYU Studies

Even as an incurable optimist, I can see the world is often drenched in suffering.

Large Genomic Differences Between Moraxella Bovoculi Isolates Acquired From The Eyes Of Cattle With Infectious Bovine Keratoconjunctivitis Versus The Deep Nasopharynx Of Asymptomatic Cattle, Aaron M. Dickey, John D. Loy, James L. Bono, Timonthy P. L. Smith, Mike D. Apley, Brian V. Lubbers, Keith D. Dedonder, Sarah F. Capik, Robert L. Larson, Brad J. White, Jochen Blom, Carol G. Chitko-Mckown, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is a recently described bacterium that is associated with infectious bovine keratoconjunctivitis (IBK) or “pinkeye” in cattle. In this study, closed circularized genomes were generated for seven M. bovoculi isolates: three that originated from the eyes of clinical IBK bovine cases and four from the deep nasopharynx of asymptomatic cattle. Isolates that originated from the eyes of IBK cases profoundly differed from those that originated from the nasopharynx of asymptomatic cattle in genome structure, gene content and polymorphism diversity and consequently placed into two distinct phylogenetic groups. These results suggest that there are genetically distinct strains of M. …

Triticum Mosaic Virus Exhibits Limited Population Variation Yet Shows Evidence Of Parallel Evolution After Replicated Serial Passage In Wheat, Melissa S. Bartels, Roy French, Robert A. Graybosch, Satyanarayana Tatineni

Department of Plant Pathology: Faculty Publications

An infectious cDNA clone of Triticum mosaic virus (TriMV) (genus Poacevirus; family Potyviridae) was used to establish three independent lineages in wheat to examine intra-host population diversity levels within protein 1 (P1) and coat protein (CP) cistrons over time. Genetic variation was assessed at passages 9, 18 and 24 by single-strand conformation polymorphism, followed by nucleotide sequencing. The founding P1 region genotype was retained at high frequencies in most lineage/passage populations, while the founding CP genotype disappeared after passage 18 in two lineages.We found that rare TriMV genotypes were present only transiently and lineages followed independent evolutionary trajectories, …

Foliar Fungicide Application Timing For Disease Management, Stay Green, And Yield In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to compare foliar fungicides applied at different corn growth stages for gray leaf spot (GLS) and southern rust (SR) efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % organic matter, and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to GLS) was planted at approximately 34,000 seed/A on 26 May, which is later than normal, due to wet spring …

Foliar Fungicide Modes Of Action For Southern Rust Management, Push Lodging, And Yield In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to compare foliar fungicides with different modes of action for southern rust (SR) efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % organic matter, and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to gray leaf spot (GLS)) was planted at approximately 34,000 seed/A on 26 May. Eight treatments were arranged in a randomized complete block design with six …

Deposition Aid Adjuvants Plus A Fungicide For Southern Rust Management, Stay Green, Push Lodging, And Yield Comparisons In Field Corn In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The objective of the trial was to compare foliar fungicides for southern rust (SR) efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % OM and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to gray leaf spot (GLS)) was planted at approximately 34,000 seed/A on 26 May. Eleven treatments were arranged in a randomized complete block design with six replications. . . . Phytotoxicity was …

Root-Lesion Nematodes, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

Root-lesion nematodes, Pratylenchus spp., are among the most commonly encountered plant-parasitic nematodes, with one or more species occurring in almost every field. Their small size (0.4-0.7 mm long) relative to most other plant-parasitic nematodes enables them to survive in almost any soil texture. Their wide host range and distribution make them one of the most damaging nematodes worldwide, ranging from cool temperate to tropical environments. At least eight species of root-lesion nematodes have been associated with sunflower.

Root-Knot Nematodes, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

Root-knot nematodes, Meloidogyne spp., are some of the most damaging plant pathogens. Several of the most common species of root-knot nematodes have been confirmed to reproduce and cause damage on sunflower, including M. arenaria, M. hapla, M. incognita, M. javanica, and M. hispanica.

Meloidogyne spp. occur worldwide but are especially common in temperate, subtropical, and tropical regions. Countries where Meloidogyne spp. have been confirmed to affect sunflower include the United States and the African nations of Egypt, Mozambique, South Africa, and Zambia. Root-knot nematodes tend to cause the most damage to plants grown in sandy …

Institutional Assessment Report, 2016, University Of Montana--Missoula. Office Of The Provost

University of Montana Planning and Assessment Reports, 1969-2016

The University of Montana 2016 Institutional Assessment Report, issued by the Office of the Provost.

Northern Corn Leaf Blight, Tamra A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

Northern corn leaf blight (NCLB) is a disease of corn caused by a fungus, Exserohilum turcicum (sexual stage Setosphaeria turcica). Its development is favored by cool to moderate temperatures and high relative humidity. Historically, NCLB has been more common and severe in states in the eastern Corn Belt, but its incidence has increased in Nebraska and the western Corn Belt in recent years. The disease is distributed throughout most of the corn-growing areas of the United States. The disease also occurs sporadically throughout other humid corn-producing areas of the world. In Nebraska, the disease has been most serious in …

Identification Of Erwinia Rhapontici As The Causal Agent Of Crown And Shoot Rot And Pink Seed Of Pea In Nebraska, Anthony O. Adesemoye, Hsin-Ho Wei, Robert M. Harveson

Department of Plant Pathology: Faculty Publications

Over the last five years, the production of dry yellow peas (Pisum sativum L.) has been increasing in Nebraska and other areas of the Central High Plains, according to a USDA report (Jasa 2013). Dry pea is a short-season crop with a low water requirement, making it a good rotational crop for the high plains. We have noted bacterial pathogens, potentially a disease complex, that may negatively impact the production of pea in this region, and one of the emerging pathogens is Erwinia rhapontici. This pathogen is a gram-negative bacterium that has been reported from soil, seed, and …

Role Of Apoptotic Hepatocytes In Hcv Dissemination: Regulation By Acetaldehyde, Murali Ganesan, Sathish Kumar Natarajan, Jinjin Zhang, Justin L. Mott, Larisa I. Poluektova, Benita L. Mcvicker, Kusum K. Kharbanda, Dean J. Tuma, Natalie A. Osna

Department of Biochemistry: Faculty Publications

Alcohol consumption exacerbates hepatitis C virus (HCV) pathogenesis and promotes disease progression, although the mechanisms are not quite clear. We have previously observed that acetaldehyde (Ach) continuously produced by the acetaldehyde-generating system (AGS), temporarily enhanced HCV RNA levels, followed by a decrease to normal or lower levels, which corresponded to apoptosis induction. Here, we studied whether Ach-induced apoptosis caused depletion of HCV-infected cells and what role apoptotic bodies (AB) play in HCV-alcohol crosstalk. In liver cells exposed to AGS, we observed the induction of miR-122 and miR-34a. As miR-34a has been associated with apoptotic signaling and miR-122 with HCV replication, …

Inhibition Of Protein Ubiquitination By Paraquat And 1-Methyl-4-Phenylpyridinium Impairs Ubiquitin-Dependent Protein Degradation Pathways, Juliana Navarro-Yepes, Anandhan Annadurai, Erin Bradley, Iryna Bohovych, Bo Yarabe, Annemieke De Jong, Huib Ovaa, You Zhou, Oleh Khalimonchuk, Betzabet Quintanilla-Vega, Rodrigo Franco

Department of Biochemistry: Faculty Publications

Intracytoplasmic inclusions of protein aggregates in dopaminergic cells (Lewy bodies) are the pathological hallmark of Parkinson’s disease (PD). Ubiquitin (Ub), alpha [α]-synuclein, p62/sequestosome 1 and oxidized proteins are major components of Lewy bodies. However, the mechanisms involved in the impairment of misfolded/oxidized protein degradation pathways in PD are still unclear. PD is linked to mitochondrial dysfunction and environmental pesticide exposure. In this work, we evaluated the effect of the pesticide paraquat (PQ) and the mitochondrial toxin 1-methyl-4-phenylpyridinium (MPP⁺) on Ub-dependent protein degradation pathways. No increase in the accumulation of Ub-bound proteins or aggregates was observed in dopaminergic cells …

Tumor Suppressive Role Of Sestrin2 During Colitis And Colon Carcinogenesis, Seung-Hyun Ro, Xiang Xue, Sadeesh K. Ramakrishnan, Chun-Seok Cho, Sim Namkoong, Insook Jang, Ian A. Semple, Allison Ho, Hwan-Woo Park, Yatrik M. Shah, Jun Hee Lee

Department of Biochemistry: Faculty Publications

The mTOR complex 1 (mTORC1) and endoplasmic reticulum (ER) stress pathways are critical regulators of intestinal inflammation and colon cancer growth. Sestrins are stress-inducible proteins, which suppress both mTORC1 and ER stress; however, the role of Sestrins in colon physiology and tumorigenesis has been elusive due to the lack of studies in human tissues or in appropriate animal models. In this study, we show that human SESN2 expression is elevated in the colon of ulcerative colitis patients but is lost upon p53 inactivation during colon carcinogenesis. In mouse colon, Sestrin2 was critical for limiting ER stress and promoting the recovery …

Potassium And The K+/H+ Exchanger Kha1p Promote Binding Of Copper To Apofet3p Multi-Copper Ferroxidase, Xiaobin Wu, Heejeong Kim, Javier Seravalli, Joseph J. Barycki, P. John Hart, David W. Gohara, Enrico Di Cera, Won Hee Jung, Daniel J. Kosman, Jaekwon Lee

Department of Biochemistry: Faculty Publications

Acquisition and distribution of metal ions support a number of biological processes. Here we show that respiratory growth of and iron acquisition by the yeast Saccharomyces cerevisiae relies on potassium (K⁺) compartmentalization to the trans-Golgi network via Kha1p, a K⁺/H⁺ exchanger. K⁺ in the trans-Golgi network facilitates binding of copper to the Fet3p multi-copper ferroxidase. The effect of K⁺ is not dependent on stable binding with Fet3p or alteration of the characteristics of the secretory pathway. The data suggest that K⁺ acts as a chemical factor in Fet3p maturation, a …

Analysis Of Oligomerization Properties Of Heme A Synthase Provides Insights Into Its Function In Eukaryotes, Samantha Swenson, Andrew Cannon, Nicholas J. Harris, Nicholas G. Taylor, Jennifer L. Fox, Oleh Khalimonchuk

Department of Biochemistry: Faculty Publications

Heme a is an essential cofactor for function of cytochrome c oxidase in the mitochondrial electron transport chain. Several evolutionarily conserved enzymes have been implicated in the biosynthesis of heme a, including the heme a synthase Cox15. However, the structure of Cox15 is unknown, its enzymatic mechanism and the role of active site residues remain debated, and recent discoveries suggest additional chaperone-like roles for this enzyme. Here, we investigated Cox15 in the model eukaryote Saccharomyces cerevisiae via several approaches to examine its oligomeric states and determine the effects of active site and human pathogenic mutations. Our results indicate that Cox15 …

Transcriptome Profiling Of Buffalograss Challenged With The Leaf Spot Pathogen Curvularia Inaequalis, Bimal S. Amaradasa, Keenan Amundsen

Department of Plant Pathology: Faculty Publications

Buffalograss (Bouteloua dactyloides) is a low maintenance U. S. native turfgrass species with exceptional drought, heat, and cold tolerance. Leaf spot caused by Curvularia inaequalis negatively impacts buffalograss visual quality. Two leaf spot susceptible and two resistant buffalograss lines were challenged with C. inaequalis. Samples were collected from treated and untreated leaves when susceptible lines showed symptoms. Transcriptome sequencing was done and differentially expressed genes were identified. Approximately 27 million raw sequencing reads were produced per sample. More than 86% of the sequencing reads mapped to an existing buffalograss reference transcriptome. De novo assembly of unmapped reads …

Morphological And Molecular Characterization Of Gracilacus Wuae N. Sp. (Nematoda: Criconematoidea) Associated With Cow Parsnip (Heracleum Maximum) In Ontario, Canada, Qing Yu, Weimin Ye, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

Gracilacus wuae n. sp. from soil associated with cow parsnip in Ontario, Canada is described and illustrated. Morphologically, females have a long stylet ranging from 80 to 93 mmlong, the lip region not offset from the body contour, without lateral lips but with large and flat submedian lobes, the mouth opening slit-like elongated laterally and surrounded by lateral flaps, the excretory pore is anterior to the knobs of the stylet; males without stylet and the pharynx degenerated. The fourth-stage juveniles lack a stylet, the pharynx degenerated, and can be differentiated into preadult females and males based on the position of …

A Research Exemption For The 21st Century, Nicholas Short

University of Michigan Journal of Law Reform Caveat

On March 20, 2015, Robert Kastenmeier, who represented Wisconsin’s Second Congressional District from 1959 to 1991, passed away at his home in Arlington, Virginia. Though Kastenmeier may not have been well known outside of legislative circles and his home state of Wisconsin, he was in fact one of the most prolific policy makers—if not the most prolific policy maker—in the field of intellectual property law in the 20th century. He is impressively credited with authoring more than forty-eight laws dealing with intellectual property matters during his legislative tenure, including the Copyright Act of 1976, which remains the primary legal framework …

¡Tigre Blanco, Héroe Del Barrio!: Living And Dying Latina/O In A Superhero World, Luis Saenz De Viguera Erkiaga

World Languages and Cultural Studies Faculty Publications

In a world where the superheroic characters from Marvel Comics and DC Comics, the two major comic book publishers in the United States, are taking over mainstream media via film, TV shows, and video-games, it seems more relevant than ever to look at the way in which these comic book fantasies deal with diversity and the representation of minorities.

Capstone Address: “Multicultural Unity, Education And Struggle: Some Essential Principles And Practices," Dr. Maulana Karenga, Csusb - Latino Education And Advocacy Days (Lead)

Latino Education and Advocacy Days (LEAD) Video Recordings

No abstract provided.