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Using Caenorhabditis Elegans As A Model For Obesity Pharmacology Development, Jolene Zheng, Joseph R. Vasselli, Jason F. King, Michael L. King, Wenqian We, Zachary Fitzpatrick, William D. Johnson, John W. Finley, Roy J. Martin, Michael J. Keenan, Frederic M. Enright, Frank L. Greenway

Faculty Publications

Copyright © 2015 Wolters Kluwer Health, Inc. The Caenorhabditis elegans model is a rapid and inexpensive method to address pharmacologic questions. We describe the use of C. elegans to explore 2 pharmacologic questions concerning candidate antiobesity drugs and illustrate its potential usefulness in pharmacologic research: (1) to determine a ratio of betahistine-olanzapine that blocks the olanzapine-induced intestinal fat deposition (IFD) as detected by Nile red staining and (2) to identify the mechanism of action of a pharmaceutical candidate AB-101 that reduces IFD. Olanzapine (53 mg/mL) increased the IFD (12.1 6 0.1%, P < 0.02), which was blocked by betahistine (763 mg/mL, 39.3 6 0.01%, P < 0.05) in wild-type C. elegans (N2). AB-101 (1.0%) reduced the IFD in N2 (P < 0.05), increased the pharyngeal pumping rate (P < 0.05), and reversed the elevated IFD induced by protease inhibitors atazanavir and ritonavir (P < 0.05). AB-101 did not affect IFD in a ACS null mutant strain acs-4(ok2872) III/hT2[bli-4(e937) let-?(q782) qIs48](I;III) suggesting an involvement of the lipid oxidation pathway and an upregulation of CPT-1. Our studies suggest that C. elegans may be used as a resource in pharmacologic research. This article is intended to stimulate a greater appreciation of its value in the development of new pharmaceutical interventions.

Daily Eastern News: November 28, 2016, Eastern Illinois University

November

No abstract provided.

Faculty Senate Bill Fsb-2016-11-28-01: University Curriculum Committee, Armstrong State University

Armstrong Faculty Senate: Bills, Motions & Resolutions (Archived)

Faculty Senate Bill FSB-2016-11-28-01: University Curriculum Committee

Robustness Of The Potential And Flux Landscapes In Fission Yeast Cell Cycle Networks, Xiaosheng Luo

Theses and Dissertations

Using fission yeast cell cycle as an example, we uncovered that the non-equilibrium network dynamics and global properties are determined by two essential features: the potential landscape and the flux landscape. While potential landscape quantifies the probabilities of different states forming hills and valleys, the flux landscape quantifies the probability fluxes of different loops flowing through states. These two landscapes can be quantified through the decomposition of the dynamics into the detailed balance preserving part and detailed balance breaking non-equilibrium part. While the funneled potential landscape is often crucial for the stability of the single attractor networks, we have uncovered …

Linking Transcriptional And Physiological Responses To High Light Stress In A Wild-Type And A Photomorphogenic Tomato Mutant, Jordyn Radke

Theses and Dissertations

Tomato (Solanum lycopersicon) is the leading vegetable crop worldwide and an important part of a healthy human diet. In this study we used Next-Generation Sequencing, metabolomics and gene ontologies to study the effects of high light stress on the photomorphogenic hp-2dg tomato mutant. Wild-type (cv. ‘Manapal’) and mutant plants were grown for six weeks under low light and transferred to high light for one week. Illumina-based RNA Sequencing was performed and analysis of differential gene expression identified over 650 transcripts that were strongly regulated by light intensity (FC>4, p2,p<0,01). HPLC analysis of tomato leaves showed differential accumulation of phenylpropanoid metabolites such as chlorogenic acid, rutin, and caffeoylglucaric acid which was strongly dependent on genotype and light condition. Our findings show insightful differences between the wildtype and the photomorphogenic mutant as well as novel genes involved in high light adaptation in plants.

November 28, 2016 Armstrong Faculty Senate Agenda, Armstrong State University

Armstrong Faculty Senate: Armstrong Faculty Senate Agenda (Archived)

November 28, 2016 Armstrong Faculty Senate Agenda

Schraut Memorial Lecture, Dave Larsen

College of Arts and Sciences Newsroom

Mathematics plays a growing role in understanding and curing disease, as the discovery and development of new drugs has shifted during the last two decades from largely empirical to heavily data driven.

Crow's Nest, November 28, 2016, Crow's Nest Staff

Crow's Nest

The November 28, 2016, issue of the Crow's Nest.

Minutes, Graduate Council Meeting, November 28, 2016, University Of South Florida St. Petersburg. Graduate Council

USF St. Petersburg Graduate Council Meetings

Minutes of the November 28, 2016, meeting of the University of South Florida St. Petersburg's Graduate Council.

Mcsa Forum Minutes 11/28/2016, Morris Campus Student Association

Morris Campus Student Association

No abstract provided.

Data Associated With 'Earlier Nesting By Predatory Bird Is Associated With Human Responses To Climate Change', Shawn Heath Smith, Karen Steenhoff, Christopher J.W. Mcclure, Julie Heath

Biological Sciences Data

Summary: 1. Warming temperatures cause changes in growing seasons and prey that drive earlier breeding by birds, especially dietary specialists within homogenous habitat. Less is known about how generalists respond to climate-associated shifts in growing seasons or prey, which may be occurring at different rates across land cover types used by predators. 2. We studied whether breeding phenology of a generalist predator, the American kestrel (Falco sparverius), in a mosaic of non-irrigated shrub/grasslands and irrigated crops and pastures, was associated with changes in growing seasons and, presumably, prey abundance. We examined the potential relationship between prey abundance (small mammals) and …

Species Distribution Model For Swift Fox In Nebraska, Lucia Corral Hurtado

Nebraska Department of Transportation: Research Reports

No abstract provided.

Problems Of Multi-Species Organisms: Endosymbionts To Holobionts, David C. Queller, Joan E. Strassmann

Biology Faculty Research

The organism is one of the fundamental concepts of biology and has been at the center of many discussions about biological individuality, yet what exactly it is can be confusing. The definition that we find generally useful is that an organism is a unit in which all the subunits have evolved to be highly cooperative, with very little conflict. We focus on how often organisms evolve from two or more formerly independent organisms. Two canonical transitions of this type—replicators clustered in cells and endosymbiotic organelles within host cells—demonstrate the reality of this kind of evolutionary transition and suggest conditions that …

November 28, 2016 Armstrong Faculty Senate Minutes, Armstrong State University

Armstrong Faculty Senate: Minutes (Archived)

November 28, 2016 Armstrong Faculty Senate Minutes

Effect Of Electrolyzed High-Ph Alkaline Water On Blood Viscosity In Healthy Adults., Joseph J. Weidman, Ralph E. Holsworth, Bradley Brossman, Daniel J. Cho, John St Cyr, Gregory Fridman

Student Papers, Posters & Projects

BACKGROUND: Previous research has shown fluid replacement beverages ingested after exercise can affect hydration biomarkers. No specific hydration marker is universally accepted as an ideal rehydration parameter following strenuous exercise. Currently, changes in body mass are used as a parameter during post-exercise hydration. Additional parameters are needed to fully appreciate and better understand rehydration following strenuous exercise. This randomized, double-blind, parallel-arm trial assessed the effect of high-pH water on four biomarkers after exercise-induced dehydration.

METHODS: One hundred healthy adults (50 M/50 F, 31 ± 6 years of age) were enrolled at a single clinical research center in Camden, NJ and …

Dissociable Early Attentional Control Mechanisms Underlying Cognitive And Affective Conflicts, Taolin Chen, Keith M. Kendrick, Chunliang Feng, Shiyue Sun, Xun Yang, Xiaogang Wang, Wenbo Luo, Suyong Yang, Xiaoqi Huang, Pedro A. Valdes-Sosa, Qiyong Gong, Jin Fan, Yue-Jia Luo

Publications and Research

It has been well documented that cognitive conflict is sensitive to the relative proportion of congruent and incongruent trials. However, few studies have examined whether affective conflict processing is modulated as a function of proportion congruency (PC). To address this question we recorded eventrelated potentials (ERP) while subjects performed both cognitive and affective face-word Stroop tasks. By varying the proportion of congruent and incongruent trials in each block, we examined the extent to which PC impacts both cognitive and affective conflict control at different temporal stages. Results showed that in the cognitive task an anteriorly localized early N2 component occurred …

The Effect Of Teaching Strategies And Learning Environment On Certification Exam Pass Rates In Dietetic Education, Debra Head

Theses and Dissertations

The obligation for preparing competent graduates begins with an educational experience in an environment that promotes optimal learning. An important outcome of successful health professions education is the passage of the certification exam. The objective of this study was to evaluate learning outcomes and teaching strategies and the effect on certification exam pass rate in dietetic education programs. A non-experimental associational cross-sectional mixed methods design was chosen for the study in order to gather objective data through the use of the previously validated Constructivist Learning Environment Survey. A 30 item electronic survey was administered to faculty in dietetic education programs …

Fluorescent Graphene For Cancer Cell Targeting And Detection, Rabab Nadeem Hamzah

Theses and Dissertations

The highest cause of death and illness around the world is cancer, and the range of cancers continues to increase. Breast cancer is the second leading cause of cancer death in women today, mainly in older women (over 40 years), younger women can also be affected, especially those with a genetic predisposition. Early detection of breast cancer is needed in order to limit the risk of this disease and make significant improvements in the rate of cure. Combining clinical trials with bionanotechnology for the diagnosis and treatment of cancer has received considerable attention in the last several years. Different nanomaterials …

Report For Nmdgf Permit: 3094, 2016, Wally Murphy

Natural Heritage New Mexico Documents

Document from New Mexico Department of Game & Fish Scientific permit.

River Otter (Lontra Canadensis) Distribution And Habitat Suitability In Nebraska, Nathan R. Bieber

School of Natural Resources: Dissertations, Theses, and Student Research

River otters (Lontra Canadensis) were extirpated in Nebraska by the early 1900’s, but in 1986, the Nebraska Game and Parks Commission (NGPC) began reintroduction efforts. Following reintroductions, the otter was listed as a tier-1 at-risk species in Nebraska. With increasing otter populations, NGPC is evaluating a de-listing plan. In order to inform de-listing efforts, I surveyed Nebraska’s rivers documenting otter sign and used modeling techniques to estimate otter distribution and habitat suitability.

Otter sign surveys were conducted on the navigable rivers of Nebraska. Occupancy modeling techniques were used to examine patterns in otter detections. The best model incorporated distance …

Preparation Ofα-Glucosidase Inhibitory Peptides Derived From Ginkgo Biloba By Enzymatic Method, Zhang Can, Wu Caie, Fan Gongjian, Li Tingting, Wang Jiahong, Gong Hao, Cao Fuliang

Food and Machinery

The preparation of α-glucosidase inhibitory peptides was gotten, which was derived from ginkgo biloba by enzymatic hydrolysis with papain. A factorial design experiment was conducted, while, the effects of concentration of substrate, pH, E/S ratio, extraction temperature and time were studied. And then, it was fractioned by using membrane ultrafiltration with molecular weight cutoffs of 100, 50, 30, 10 and 3 kDa. The results showed: with the concentration of substrate 1.5%, pH 7.36, E/S ratio of 5 g/100 g, temperature of 57.9 ℃ and extraction time of 4.4 h, the inhibitory activities ofα-glucosidase was the highest, and reached 17.18%. The …

Research And Development Of Functional Properties In Pueraria, Liang Jie, Li Lin, Tang Hanjun

Food and Machinery

Summarized the functional properties and application of pueraria in the food industry, and analyzed the exploitation, research and problems of the kudzu as a dry crop resource. It was helpful for high efficiency development and level improvement in technology and industry development of pueraria application.

Diversity And Impacts Of Mining On The Non-Volant Small Mammal Communities Of Two Vegetation Types In The Brazilian Amazon, Natália Carneiro Ardente, Átilla Colombo Ferreguetti, Donald Gettinger, Pricila Leal, Ana Cristina Mendes-Oliveira, Fernanda Martins-Hatano, Helena Godoy Bergallo

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The CarajaÂs National Forest contains some of the largest iron ore deposits in the world. The majority of the minerals are found below a plant community known as Savana Metalo fila, or ªCangaº, which represents only 3% of the landscape within the CarajaÂs National Forest (CNF). The aim of our study was to understand the diversity of community of non-volant small mammals in the two predominant vegetation types: Ombrophilous Forest and Canga, and to examine how mining impacts these communities. Sampling was conducted from January 2010 to August 2011 in 11 sampling sites divided by the total area of Canga …

Solid Stress And Elastic Energy As Measures Of Tumour Mechanopathology, Hadi T. Nia, Hao Liu, Giorgio Seano, Meenal Datta, Dennis Jones, Nuh Rahbari, Joao Incio, Vikash P. Chauhan, Keehoon Jung, John D. Martin, Vasileios Askoxylakis, Timothy P. Padera, Dai Fukumura, Yves Boucher, Francis J. Hornicek, Alan J. Grodzinsky, James W. Baish, Lance L. Munn, Rakesh K. Jain

Faculty Journal Articles

olid stress and tissue stiffness affect tumour growth, invasion, metastasis and treatment. Unlike stiffness, which can be precisely mapped in tumours, the measurement of solid stresses is challenging. Here, we show that 2D spatial maps of the solid stress and the resulting elastic energy in excised or in situ tumours with arbitrary shapes and a wide range of sizes can be obtained via three distinct and quantitative techniques that rely on the measurement of tissue displacement after disruption of the confining structures. Application of these methods in models of primary tumours and metastasis revealed that (i) solid stress depends on …

Using Geochemistry And Strontium Isotopic Ratios (87sr/86sr) To Determining Water Mixing And Sources In The Picher Mining District, William Daniel Ketcheside

Theses and Dissertations

The mining of lead and zinc ores in the Tri-State Mining District of Kansas, Missouri, and Oklahoma resulted in some of the most severe environmental degradation in the country. Large amounts of waste rock, known as ‘chat’, were produced during mining and left in surface piles in the surrounding area. These chat piles, located mostly near the town of Picher, Oklahoma contain potentially harmful elements that can be leached from the rock into local streams and sediment. The mines also filled with water after mining ceased and have discharged contaminated water to nearby waterways, as well as resulted in groundwater …

Cortisol In Human Milk Predicts Child Bmi, Jennifer Hahn-Holbrook, Tran Bao Le, Anna Chung, Elyssia Poggi Davis, Laura M. Glynn

Psychology Faculty Articles and Research

Objective—Breastfeeding is linked to lower rates of childhood obesity. Human milk contains cortisol, known to regulate glucose storage and metabolism. We aimed to test the hypothesis that early exposure to cortisol in human breast milk helps to modulate infant BMI trajectories over the first two years of life.

Methods—Growth curve modeling was used to examine whether infant exposure to cortisol in human milk at 3 months predicted changes in child body mass index percentile (BMIP) at 6, 12, and 24 months of age in 51 breastfeeding mother-child pairs.

Results—Infants exposed to higher milk cortisol levels at 3 …

Optimization On Ultra High Pressure Extraction Process Of Mussel Polysaccharide Based Artificial Neural Network, Zhang Bin, Sun Lanping, Shi Ying, Tu Kang

Food and Machinery

Based on the raw materials of mussel meat after taking out the pearl, ultra high pressure is used to extract mussel polysaccharide. The response surface experimental design was used to get the training samples for the neutral network, while the sophisticated neutral network was used for training and stimulating, analyzing extraction factors (pressure strength, solid- liquid ratio and pressure holding time), the interaction between the factors that affected the extraction rate of mussel polysaccharide, and optimizing the ultra high pressure extraction process of mussel polysaccharide. The results showed that artificial neutral network optimization was more accurate than response surface method …

Effect Of Core Browning And Storage Fresh-Keeping On Huangguan Pear By Low-Power Microwave Treatment, Guo Yanming, Dong Ming, Zhang Linyu, Wu Xiaocong, Zhang Shuyuan, Jin Wenping

Food and Machinery

With a low-power microwave treatment on Huangguan pear fruits, different levels of low-power microwave treatment on fruit storage quality and the influence of the core browning were studied. Through the determinations of total phenols, polyphenol oxidase (PPO), malondialdehyde (MDA), DPPH in Huangguan pear core and ethylene, respiration intensity, hardness in storage period, the optimal treating conditions were gotten. Under the condition of 0～2 ℃ refrigerating, compared with the control group, the low energy microwave treatment group could keep the fruits of high hardness, delay the reduce of soluble solids, reduce the respiration intensity of fruit, and delay the ethylene peak …

Study On Extraction Of Essential Oil From Lysimachia Foenum-Graecum Hance And Its Preservation Effect On Longan, Yan Xuyu, Li Ling, Chen Ting, Wu Yuanjiao

Food and Machinery

Essential oil was extracted from Lysimachia foenum-graecum Hance using steam distillation method. The extraction conditions of essential oil were optimized by orthogonal experimental design, and its preservation on longan was studied. The results showed that the optimum extraction technology were: the concentration of sodium chloride 4%, the distilling time was 6 h, and the solid-liquid ratio of 1∶10 (g/mL). Under the condition, the extraction rate of essential oil reached 0.167 2%. The preservation results indicated: the antibacterial effects of 4 treatments, the content of Vitamin C and soluble solid decreased gradually, and weight loss and corruption rate of longan increased …

Simultaneous Detection Of Puffiness And Soluble Solids Content For Intact Citrus By Online Spectroscopy, Liu Yande, Wu Mingming, Sun Xudong, Zhu Danning

Food and Machinery

Puffy fruit and soluble solid content (SSC) are important indexes for evaluating the quality of citrus. The feasibility was discussed for detecting Puffing disease and SSC of intact citrus simultaneously by online visible-near infrared (visible-NIR) transmittance spectroscopy. The spectra were recorded with the integration time of 100 ms in the wavelength range of 350~1 150 nm when the samples were conveyed at the speed of five samples per second. The feasibility of simultaneous and online detection of puffiness fruit and SSC for intact citrus simultaneously was discussed by visible-near infrared transmittance spectroscopy. The response properties of visible-NIR spectra for normal …