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2016

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Articles 18931 - 18960 of 29103

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Collagen/Peo/Gold Nanofibrous Matrices For Skin Tissue Engineering, Ömer Aktürk, Di̇lek Keski̇n Jan 2016

Collagen/Peo/Gold Nanofibrous Matrices For Skin Tissue Engineering, Ömer Aktürk, Di̇lek Keski̇n

Turkish Journal of Biology

As a novel approach in skin tissue engineering, gold nanoparticles (AuNPs) were synthesized and incorporated at different concentrations into collagen/PEO nanofibrous matrices in this study. The group containing 14.27 ppm AuNPs (CM-Au) had the best nanofibrous morphology. CM-Au was cross-linked with glutaraldehyde vapor (CM-AuX). All groups were disrupted in collagenase in 2 h, but cross-linked groups and Matriderm1397904493 resisted hydrolytic degradation for 7 and 14 days, respectively. Due to its small pores and dense structure, lower water swelling results (7.26 ± 2.62 g/g) were obtained for CM-AuX than Matriderm (17.51 ± 1.97 g/g). CM-Au and Matriderm had statistically similar tensile …

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Triterpenoid Saponin-Rich Fraction Of Centella Asiatica Decreases Il-1ss Andnf-?B, And Augments Tissue Regeneration And Excision Wound Repair, Amena Mahmood, Ambrish Tiwari, Kazi̇m Şahi̇n, Ömer Küçük, Shakir Ali Jan 2016

Triterpenoid Saponin-Rich Fraction Of Centella Asiatica Decreases Il-1ss Andnf-?B, And Augments Tissue Regeneration And Excision Wound Repair, Amena Mahmood, Ambrish Tiwari, Kazi̇m Şahi̇n, Ömer Küçük, Shakir Ali

Turkish Journal of Biology

Wound healing is an orderly process driven by numerous cellular mediators that aim to accelerate it. In skin conditions such as leprosy and psoriasis, Centella asiatica, which contains high amounts of triterpenoid saponins (TSs), has been reported to have beneficial effects. This study examined the effects of TS fraction of the herb on tissue regeneration and excision wound repair in rat skin. Briefly, TS fraction was prepared from C. Asiatica and applied on the wound area for 7 consecutive days. The IL-1β, NF-κB, and other biochemical parameters were then estimated, followed by examination of inflammatory cell infiltration, epithelialization, angiogenesis, and …

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The Sca-1+ Mesenchymal Stromal Cells Modulate Macrophage Commitment And Function, Yaozhen Chen, Yan Chen, Dandan Yin, Yazhou Wang, Zheng Liu, Ning An, Fan Wen, Na Li, Jiajia Xin, Xingbin Hu, Huijie Zhang, Wen Yin Jan 2016

The Sca-1+ Mesenchymal Stromal Cells Modulate Macrophage Commitment And Function, Yaozhen Chen, Yan Chen, Dandan Yin, Yazhou Wang, Zheng Liu, Ning An, Fan Wen, Na Li, Jiajia Xin, Xingbin Hu, Huijie Zhang, Wen Yin

Turkish Journal of Biology

Mesenchymal stromal cells (MSCs) have been characterized as an important component of the hematopoietic niche that plays a role in regulating hematopoietic stem cell (HSC) quiescence, self-renewal, and lineage commitment. Macrophages, one kind of innate immune cells, are mainly developed from HSCs in niche. However, how MSCs and their subpopulations regulate macrophage commitment is still unknown. The current study compared the contribution of MSCs and Sca-1+MSCs to macrophage commitment and further modulation on the function of macrophages. We found that Sca-1+MSCs could promote macrophage commitment through the M-CSFR gene, and Sca-1+MSCs led macrophage polarization towards the M2 phenotype. Further functional …

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Role Of Melatonin On Differentiation Of Mesenchymal Stem Cells Derived From Third Molar Germ Tissue, Nurullah Aydoğdu, Paki̇ze Nesli̇han Taşli, Hati̇ce Burcu Şi̇şli̇, Mehmet Emi̇r Yalvaç, Fi̇kretti̇n Şahi̇n Jan 2016

Role Of Melatonin On Differentiation Of Mesenchymal Stem Cells Derived From Third Molar Germ Tissue, Nurullah Aydoğdu, Paki̇ze Nesli̇han Taşli, Hati̇ce Burcu Şi̇şli̇, Mehmet Emi̇r Yalvaç, Fi̇kretti̇n Şahi̇n

Turkish Journal of Biology

Stem cell-based applications have become a popular and promising approach for therapy for a number of disorders including neurodegenerative diseases, degenerative muscle diseases, and osteoporosis, as well as trauma, inflammations, burns, and injuries. Human tooth germ stem cells are an adult stem cell source; they have mesenchymal stem cell properties and show high proliferative and differentiation capacity. Melatonin has been demonstrated to regulate differentiation of human and mouse mesenchymal stem cells into various cell lineages in addition to its other functions in the body. In the current study, the effects of melatonin on osteogenic, neurogenic, adipogenic, chondrogenic, myogenic, and odontogenic …

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The Restorative Effect Of Ascorbic Acid On Liver Injury Inducedby Asymmetric Dimethylarginine, Ali̇ Okuyucu, Osman Şaliş, Ömer Alici, Abdullah Güvenli̇, Yüksel Terzi̇, Muhammed Emi̇n Keleş, Fati̇h İlkaya, İbrahi̇m Gören, Hasan Alaçam Jan 2016

The Restorative Effect Of Ascorbic Acid On Liver Injury Inducedby Asymmetric Dimethylarginine, Ali̇ Okuyucu, Osman Şaliş, Ömer Alici, Abdullah Güvenli̇, Yüksel Terzi̇, Muhammed Emi̇n Keleş, Fati̇h İlkaya, İbrahi̇m Gören, Hasan Alaçam

Turkish Journal of Biology

We aimed to determine whether increased levels of asymmetric dimethylarginine (ADMA) directly cause hepatic deterioration and whether it is decreased by ascorbic acid (AA) as an antioxidant. The study included three groups: Control (n = 10), ADMA (n = 10), and ADMA + AA (n = 10). ADMA was administered at 2 mg kg-1 day-1 and AA was given at 50 mg kg-1 day-1 for 10 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase, and alkaline phosphatase activities were analyzed. Histomorphological evaluation of the liver was performed based on the Ishak scoring system. When compared with the control, …

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Construction Of A Tissue-Engineered Human Corneal Endothelium And Its Transplantation In Rabbit Models, Jun Zhao, Xiya Ma, Tingjun Fan Jan 2016

Construction Of A Tissue-Engineered Human Corneal Endothelium And Its Transplantation In Rabbit Models, Jun Zhao, Xiya Ma, Tingjun Fan

Turkish Journal of Biology

Human corneal endothelium (HCE) is vital in maintaining corneal transparency and thickness, and damages to it may result in endotheliopathy and even corneal blindness, which can only be cured by keratoplasty. Due to the shortage of donor corneas, tissue-engineered HCE (TE-HCE) has become an equivalent to HCE. This study constructs a high endothelial cell density (ECD) TE-HCE and evaluates its functions by corneal transplantation in rabbits. A TE-HCE was constructed by culturing DiI-labeled nontransfected HCE cells on modified denuded amniotic membrane (mdAM) using collagen IV, fibronectin, and laminin, and rabbit corneas were characterized both in vivo and ex vivo after …

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Evaluation Of Biocompatibility Of Random Or Aligned Electrospun Polyhydroxybutyrate Scaffolds Combined With Human Mesenchymal Stem Cells, Sevi̇l Köse, Fati̇ma Aerts Kaya, Emi̇r Baki̇ Denkbaş, Petek Korkusuz, Fahri̇ye Duygu Çeti̇nkaya Jan 2016

Evaluation Of Biocompatibility Of Random Or Aligned Electrospun Polyhydroxybutyrate Scaffolds Combined With Human Mesenchymal Stem Cells, Sevi̇l Köse, Fati̇ma Aerts Kaya, Emi̇r Baki̇ Denkbaş, Petek Korkusuz, Fahri̇ye Duygu Çeti̇nkaya

Turkish Journal of Biology

Polyhydroxybutyrate (PHB) is a polymer used to restore tissues or regenerate bones. However, its compatibility with human bone marrow-derived mesenchymal stem cells (MSCs) has not been examined. PHB membranes of random (r-PHB) or aligned (a-PHB) electrospun nanofibers that generate bone and spinal axon scaffolds were combined with human MSCs. The adhesion and proliferation of cells on these membranes were examined. The orientation of cells on PHB membranes was analyzed by confocal microscopy and scanning electron microscopy (SEM). The MSCs maintained their characteristic properties on the membranes, adhered to the membranes, and preserved their viability. The cell morphology was different when …

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Third-Party Wharton?S Jelly Mesenchymal Stem Cells For Treatment Of Steroid-Resistant Acute And Chronic Graft-Versus-Host Disease: A Report Of 10 Cases, Dariusz Boruczkowski, Dominika Gladysz, Slawomir Ruminski, Iwona Czaplicka-Szmaus, Magdalena Murzyn, Artur Olkowicz, Krzysztof Kalwak, Monika Mielcarek, Katarzyna Drabko, Jan Styczynski, Miroslaw Markiewicz, Katarzyna Pawelec, Maciej Boruczkowski, Tomasz Oldak Jan 2016

Third-Party Wharton?S Jelly Mesenchymal Stem Cells For Treatment Of Steroid-Resistant Acute And Chronic Graft-Versus-Host Disease: A Report Of 10 Cases, Dariusz Boruczkowski, Dominika Gladysz, Slawomir Ruminski, Iwona Czaplicka-Szmaus, Magdalena Murzyn, Artur Olkowicz, Krzysztof Kalwak, Monika Mielcarek, Katarzyna Drabko, Jan Styczynski, Miroslaw Markiewicz, Katarzyna Pawelec, Maciej Boruczkowski, Tomasz Oldak

Turkish Journal of Biology

Severe graft-versus-host disease (GvHD) is a life-threatening complication after allogeneic hematopoietic stem cell transplantation (HSCT). Bone marrow mesenchymal stem cells (BM-MSCs) display immunosuppressive activity and have been previously shown to improve the outcome of GvHD therapy. We describe the use of umbilical cord mesenchymal stem cells (WJ-MSCs) expanded in serum-free medium for the treatment of acute GvHD (aGvHD) and chronic GvHD (cGvHD) in 10 patients. After 1 to 3 WJ-MSC infusions at a median dose of 1.5 × 106 cells/kg recipient body weight, a complete or partial response was observed in 4/7 patients with aGvHD and in 2/3 patients with …

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Osteogenic Differentiation Of Electrostimulated Human Mesenchymal Stem Cells Seeded On Silk-Fibroin Films, Anil Sera Çakmak, Soner Çakmak, James White, Waseem Raja, David Kaplan, Menemşe Gümüşdereli̇oğlu Jan 2016

Osteogenic Differentiation Of Electrostimulated Human Mesenchymal Stem Cells Seeded On Silk-Fibroin Films, Anil Sera Çakmak, Soner Çakmak, James White, Waseem Raja, David Kaplan, Menemşe Gümüşdereli̇oğlu

Turkish Journal of Biology

Electric field is known as an important regulator to guide the development and regeneration of many tissues. The aim of this study was to investigate the osteogenic differentiation potential of human mesenchymal stem cells (hMSCs) cultivated on silk-fibroin films in response to different parameters, i.e. Frequency, voltage, distance between electrodes, and/or culture conditions (growth medium or osteogenic medium). Silk films were prepared in the presence of platinum wires to study the impact of exogenous electrostimulation on the cells for up to 14 days. The experimental groups can be defined as high voltage in osteogenic differentiation medium, low voltage in osteogenic …

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Mesenchymal Stem Cells Transplantation Delays Functional Deteriorationby Inhibiting Neuroinflammation Response In Aged Mice, Yi Lu Ye, Ke Yu, Xuan Dan Lin, Ying Wang, Li Jun Wu, Jia Ni Xu, Dan Dan Liu, Kai Zhong, Qi Zhang, Gui Yuan Lv Jan 2016

Mesenchymal Stem Cells Transplantation Delays Functional Deteriorationby Inhibiting Neuroinflammation Response In Aged Mice, Yi Lu Ye, Ke Yu, Xuan Dan Lin, Ying Wang, Li Jun Wu, Jia Ni Xu, Dan Dan Liu, Kai Zhong, Qi Zhang, Gui Yuan Lv

Turkish Journal of Biology

This study investigated the effect of transplantation of bone marrow mesenchymal stem cells (BM-MSCs) on functional deterioration and neuroinflammatory response in aged C57BL/6J mice. Mice were divided into 3 groups: normal aged group, saline control group, and BM-MSCs treatment group. Saline and BM-MSCs were administrated by intraventricular injection into the right ventricle. Spatial Y-maze (SYM) test, novel objective recognition (NOR) test, and locomotor activity test were used to evaluate cognitive and locomotor ability at 7 and 42 days after transplantation. Microglia and astrocyte expression in the hippocampus CA1 region and dentate gyrus (DG) were evaluated by immunohistochemical method. Furthermore, the …

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Simvastatin Combined With Bone Marrow Stromal Cells Treatment Activatesastrocytes To Ameliorate Neurological Function After Ischemic Stroke In Rats, Yonghua Zhao, Qian Zhang, Zhenwei Chen, Naiwei Liu, Chienchih Ke, Youhua Xu, Weikang Wu Jan 2016

Simvastatin Combined With Bone Marrow Stromal Cells Treatment Activatesastrocytes To Ameliorate Neurological Function After Ischemic Stroke In Rats, Yonghua Zhao, Qian Zhang, Zhenwei Chen, Naiwei Liu, Chienchih Ke, Youhua Xu, Weikang Wu

Turkish Journal of Biology

Evidence indicates that reactive astrocytes play an important role in neurological functional amelioration after stroke. Bone marrow stromal cell (BMSC) transplantation-promoted astrocyte-derived neurotrophic factors' expressions resulted in decreasing the number of apoptotic neurons and improving neurogenesis and angiogenesis in the ischemic boundary zone. Recent studies found that simvastatin had neuroprotective activity in acute stroke. However, it is still ambiguous as to whether simvastatin combined with BMSCs can further activate astrocytes, which contribute to neurological functional recovery after ischemic stroke. In our present study, we established a middle cerebral artery occlusion model in rats and found that combination treatment of simvastatin …

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Salinity Effects On Expression Of Some Important Genes In Sensitiveand Tolerant Grape Genotypes, Nayer Mohammadkhani, Reza Heidari, Nasser Abbaspour, Fatemeh Rahmani Jan 2016

Salinity Effects On Expression Of Some Important Genes In Sensitiveand Tolerant Grape Genotypes, Nayer Mohammadkhani, Reza Heidari, Nasser Abbaspour, Fatemeh Rahmani

Turkish Journal of Biology

The effects of NaCl on expression of some genes related to salinity tolerance in grape (Vitis L.) were investigated. According to our screening study on eighteen grape genotypes, H6 and Gharashani (tolerant) and Shirazi and GhezelUzum (sensitive) were selected for molecular analysis. Our tolerant genotypes showed a higher water potential and a lower MDA content compared to other genotypes. Plants were treated with 50 mM NaCl as a critical concentration that is not lethal for grape. The expression profile of VvNHL1 in leaves of all genotypes and in roots of tolerant genotypes was similar to that of VvEDS1; these genes …

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Physiological Responses Of Water Hyacinth, Eichhornia Crassipes (Mart.) Solms,To Cadmium And Its Phytoremediation Potential, Suchismita Das, Sunayana Goswami, Anupam Das Talukdar Jan 2016

Physiological Responses Of Water Hyacinth, Eichhornia Crassipes (Mart.) Solms,To Cadmium And Its Phytoremediation Potential, Suchismita Das, Sunayana Goswami, Anupam Das Talukdar

Turkish Journal of Biology

Eichhornia crassipes is an abundant floating aquatic weed that has great potential for cadmium (Cd) remediation owing to its large biomass and relatively high tolerance and accumulation capabilities. This study was conducted with Eichhornia in 5, 10, 15, and 20 mg L-1 CdCl2 in a hydroponic system for 21 days, and the Cd concentrations in the roots, shoots, and leaves were estimated. The plant showed tolerance, but at high Cd concentrations declines in biomass, root length, and leaf area were observed. Leaves showed a progressive decline in chlorophyll, carotenoid, and soluble protein and a significant elevation in lipid peroxidation. Cd …

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Prediction Of Glycation Sites: New Insights From Protein Structural Analysis, Homero Saenz Suarez, Raul A Poutou Piñales, Janneth Gonzalez Santos, George E. Barreto, Lynda Patricia Prieto Navarrera, Jose A . Saenz Moreno, Patricia Landazuri, Luis A Barrera Avellaneda Jan 2016

Prediction Of Glycation Sites: New Insights From Protein Structural Analysis, Homero Saenz Suarez, Raul A Poutou Piñales, Janneth Gonzalez Santos, George E. Barreto, Lynda Patricia Prieto Navarrera, Jose A . Saenz Moreno, Patricia Landazuri, Luis A Barrera Avellaneda

Turkish Journal of Biology

Glycation of proteins is a nonenzymatic process in which proteins react with reducing sugar molecules. This process takes place at ?-amino (?-NH+) groups of lysine or hydroxylysine residues as well as α-amino groups of N-terminal residues. In the present study glycation of ?-NH+ groups of lysines was computationally analyzed for 26 proteins based on their 3D structures. We determined the spatial relationship with acidic or basic residues and correlated them with the glycation prediction algorithm Netglycate 1.0 software, which employed primary structure exclusively for glycation site prediction. Of the lysines from 19 of the 20 proteins employed to build the …

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Identification And Prediction Of The Consequences Of Nonsynonymous Snps In Glyceraldehyde 3-Phosphate Dehydrogenase (Gapdh) Gene Of Zebrafish Danio Rerio, Kiran D. Rasal, Vemulawada Chakrapani, Swagat K. Patra, Sasmita Jena, Shibani D. Mohapatra, Swapnarani Nayak, Jitendra K. Sundaray, Pallipuram Jayasankar, Hirak K. Barman Jan 2016

Identification And Prediction Of The Consequences Of Nonsynonymous Snps In Glyceraldehyde 3-Phosphate Dehydrogenase (Gapdh) Gene Of Zebrafish Danio Rerio, Kiran D. Rasal, Vemulawada Chakrapani, Swagat K. Patra, Sasmita Jena, Shibani D. Mohapatra, Swapnarani Nayak, Jitendra K. Sundaray, Pallipuram Jayasankar, Hirak K. Barman

Turkish Journal of Biology

The recent progress in DNA sequencing and computational algorithms dramatically heightened the value of single nucleotide polymorphism (SNP) databases. The experimental studies of mutation for the particular gene of interest are laborious and time-consuming. In this study, we used several computational algorithms to investigate the structural and functional consequences of SNPs on the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene of zebrafish (Danio rerio). The GAPDH gene is involved in multiple cellular activities, in addition to its association with classical glycolytic pathway. It has most commonly been used as the housekeeping gene for differential gene expression profiling, including western blots. The computational …

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Molecular Cloning Of Lipocalin-2 Into A Eukaryotic Vector And Its Expression Inbovine Mammary Epithelial Cells As A Potential Treatment For Bovine Mastitis, Neelesh Sharma, Simrinder Singh Sodhi, Jeong Hyun Kim, Mrinmoy Ghosh, Jiao Jiao Zhang, Deog Bon Koo, Man Jong Kang, Dong Kee Jeong Jan 2016

Molecular Cloning Of Lipocalin-2 Into A Eukaryotic Vector And Its Expression Inbovine Mammary Epithelial Cells As A Potential Treatment For Bovine Mastitis, Neelesh Sharma, Simrinder Singh Sodhi, Jeong Hyun Kim, Mrinmoy Ghosh, Jiao Jiao Zhang, Deog Bon Koo, Man Jong Kang, Dong Kee Jeong

Turkish Journal of Biology

Lipocalin-2 (LCN2) is a 25-kDa protein in the lipocalin family that shows antibacterial activity. The aim of this study was to clone human LCN2 into the mammalian-specific pIRES2-AcGFP1 vector and to determine the antibacterial activity of the recombinant pIRES2-AcGFP1-hLCN2 plasmid against major bacterial pathogens that cause bovine mastitis. The amplified hLCN2 was successfully cloned into the vector, and the plasmid was transfected into bovine mammary epithelial stem cells using nucleofection. Immunochemistry, reverse transcription polymerase chain reaction, and ELISA were used to monitor and quantify hLCN2 expression in the cells. After 1 day of incubation, the concentration of hLCN2 secreted by …

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Overexpression Of An H+-Ppase Gene From Arabidopsis In Sugarcane Improvesdrought Tolerance, Plant Growth, And Photosynthetic Responses, Ghulam Raza, Kazim Ali, Muhammad Yasin Ashraf, Shahid Mansoor, Muhammad Javid, Shaheen Asad Jan 2016

Overexpression Of An H+-Ppase Gene From Arabidopsis In Sugarcane Improvesdrought Tolerance, Plant Growth, And Photosynthetic Responses, Ghulam Raza, Kazim Ali, Muhammad Yasin Ashraf, Shahid Mansoor, Muhammad Javid, Shaheen Asad

Turkish Journal of Biology

This study investigated the integration of Arabidopsis vacuolar H+-pyro-phosphatase (H+-PPase) (AVP1) transgene in transgenic sugarcane plants for drought tolerance. The gene integration was confirmed by polymerase chain reaction (PCR) and Southern blotting. Transgene expression was estimated by Western blotting. When sugarcane plants were grown on soil under 50% reduced water supply, the transgenic sugarcane overexpressing AVP1 produced higher shoot biomass based on cane height, number of millable canes, and Brix (%) compared with wild-type. The overexpression of AVP1 in transgenic sugarcane plants increased tolerance to drought stress, as demonstrated by increased relative water content (RWC) and leaf water (?w), osmotic …

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Order-Wide In Silico Comparative Analysis And Identification Ofgrowth-Regulating Factor Proteins In Malpighiales, Murat Kemal Avci, Muavvi̇z Ayvaz, Hüseyi̇n Uysal, Emre Sevi̇ndi̇k, Seda Örenay Boyacioğlu, Çi̇ğdem Yamaner Jan 2016

Order-Wide In Silico Comparative Analysis And Identification Ofgrowth-Regulating Factor Proteins In Malpighiales, Murat Kemal Avci, Muavvi̇z Ayvaz, Hüseyi̇n Uysal, Emre Sevi̇ndi̇k, Seda Örenay Boyacioğlu, Çi̇ğdem Yamaner

Turkish Journal of Biology

Malpighiales, containing approximately 16,000 species, is one of the largest and most diverse angiosperm orders in plants. Growth-regulating factor (GRF) protein is a putative transcription factor and plays a regulatory role during plant growth and development processes such as stem elongation and cell expansion. The latest available protein data provide an opportunity to compare and understand the critical similarities and differentiation in GRF proteins among Malpighiales members. In the present study we conducted domain, two- and three-dimensional (2D, 3D) comparative, physicochemical, subcellular prediction, and sequence analysis of 87 putative GRF proteins belonging to 4 genera in Malpighiales using different bioinformatic …

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Proteomics Comparison Of Aspartic Protease Enzyme In Insects, Samin Seddigh, Maryam Darabi Jan 2016

Proteomics Comparison Of Aspartic Protease Enzyme In Insects, Samin Seddigh, Maryam Darabi

Turkish Journal of Biology

Aspartic proteases (APs; EC: 3.4.23) are a catalytic type of protease enzymes that use an activated water molecule, bound to one or more aspartate residues, for catalysis of their peptide substrates. In this study, bioinformatic analyses of APs enzymes were performed on insect protein sequences, including nineteen species of eleven different families. According to the conserved motifs obtained with MEME and MAST tools, three motifs were common to all insects. The structural and functional analyses of five selected insects from different orders were performed with ProtParam, SOPMA, SignalP 4.1, TMHMM 2.0, and ProDom tools in the ExPASy database. The tertiary …

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The Cytotoxic Activity Of Sanguinarine In C32 Human Amelanotic Melanoma Cells, Sabina Ioana Cojocaru, Miruna Silvia Stan, Mihai Anton, Mihaela Rocsana Luca, Gogu Ghiorghita, Gheorghe Stoian, Anca Dinischiotu Jan 2016

The Cytotoxic Activity Of Sanguinarine In C32 Human Amelanotic Melanoma Cells, Sabina Ioana Cojocaru, Miruna Silvia Stan, Mihai Anton, Mihaela Rocsana Luca, Gogu Ghiorghita, Gheorghe Stoian, Anca Dinischiotu

Turkish Journal of Biology

Sanguinarine, an alkaloid present in various medicinal plants, has been associated with detrimental effects on different types of cancer cells. The aim of our study was to analyze the cytotoxic effects of sanguinarine on C32 human amelanotic melanoma cells. The cells were exposed to various concentrations of sanguinarine (0.5, 1, and 2 μM) for 24 h and displayed a dose-dependent decrease in cell viability; the sanguinarine-induced oxidative stress in C32 cells affected the activity of antioxidant enzymes and generated lipid peroxidation. Moreover, we observed an enhanced expression of heat shock proteins 60, 70, and 90 in response to the stress …

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Genotoxic Potential Of Cynanchum Sarcomedium Meve & Liede Coupled With Its Modulatory Action On Oxidative-Stress?Mediatedgenotoxicity By Hydrogen Peroxideneethu Kannan Bhagyanathan*, John Ernest Thoppilcell And Molecular Biology Laboratory, Department Of Botany, University Of Calicut, Kerala, India, Neethu Kannan Bhagyanathan, John Ernest Thoppil Jan 2016

Genotoxic Potential Of Cynanchum Sarcomedium Meve & Liede Coupled With Its Modulatory Action On Oxidative-Stress?Mediatedgenotoxicity By Hydrogen Peroxideneethu Kannan Bhagyanathan*, John Ernest Thoppilcell And Molecular Biology Laboratory, Department Of Botany, University Of Calicut, Kerala, India, Neethu Kannan Bhagyanathan, John Ernest Thoppil

Turkish Journal of Biology

The present study evaluated the genotoxic effect of aqueous extract of C. sarcomedium Meve and Liede and its protective role in chromosomal aberrations induced by 2% hydrogen peroxide (H2O2) on Allium cepa root meristem. Our results showed that the C. sarcomedium extract induced major chromosomal aberrations, viz., nuclear lesions, chromosome breaks, chromosome laggards, vagrance, stickiness, and bridges. These are irreversible in nature and indicate that the plant is a potent clastogen. Total chromosome aberrations were more frequent with increase in exposure time and concentrations of plant extract. For antigenotoxic screening, three different modes of treatment were used: pretreatment, posttreatment, and …

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Overexpression Of A Fusion Defensin Gene From Radish And Fenugreek Improvesresistance Against Leaf Spot Diseases Caused By Cercospora Arachidicola And Phaeoisariopsis Personata In Peanut, Madhu Bala, Thankappan Radhakrishnan, Abhay Kumar, Gyan Prakash Mishra, Jentilal Ramjibhai Dobraia, Pulugurtha Bharadwaja Kirti Jan 2016

Overexpression Of A Fusion Defensin Gene From Radish And Fenugreek Improvesresistance Against Leaf Spot Diseases Caused By Cercospora Arachidicola And Phaeoisariopsis Personata In Peanut, Madhu Bala, Thankappan Radhakrishnan, Abhay Kumar, Gyan Prakash Mishra, Jentilal Ramjibhai Dobraia, Pulugurtha Bharadwaja Kirti

Turkish Journal of Biology

Peanut (Arachis hypogaea L.) is one of the important oilseed crops of the Indian subcontinent and fungal diseases like early leaf spot (ELS) and late leaf spot (LLS) caused by Cercospora arachidicola and Phaeoisariopsis personata, respectively, are major peanut cultivation constraints. Defensins are basically antimicrobial peptides that have been implicated in plant defense against various microbial attacks. Transgenic peanut plants, developed through Agrobacterium mediated transformation of de-embryonated cotyledons and overexpressing a synthetic defensin fusion gene from fenugreek (Tfgd2) and radish (RsAFP2) linked by a linker peptide, were found to have enhanced resistance to the ELS and LLS infection over the …

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Control Of Tomato Yellow Leaf Curl Virus Disease By Enterobacter Asburiaebq9 As A Result Of Priming Plant Resistance In Tomatoes, Hongwei Li, Xueling Ding, Chao Wang, Hongjiao Ke, Zhou Wu, Yupeng Wang, Hongxia Liu, Jianhua Guo Jan 2016

Control Of Tomato Yellow Leaf Curl Virus Disease By Enterobacter Asburiaebq9 As A Result Of Priming Plant Resistance In Tomatoes, Hongwei Li, Xueling Ding, Chao Wang, Hongjiao Ke, Zhou Wu, Yupeng Wang, Hongxia Liu, Jianhua Guo

Turkish Journal of Biology

Enterobacter asburiae BQ9, a plant-growth-promoting rhizobacterium, was shown to promote tomato plant growth and induce resistance to Tomato yellow leaf curl virus (TYLCV) under greenhouse conditions. Compared with mock-treated tomato plants, plants that were pretreated with BQ9 had increased fresh mass and significantly reduced disease severity and 52% biocontrol efficacy was achieved 30 days after inoculation. The expression of defense-related genes PR1a and PR1b and the H2O2 burst were quickly induced in BQ9 pretreated plants. Antioxidase activity analysis showed that the activities of phenylalanine ammonia lyase, peroxidase, catalase, and superoxide dismutase increased significantly in BQ9 pretreated plants. Our results suggest …

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Analysis Of Protein Expression Changes In The Blood Plasma Of Cows During The Last Month Before Parturition And 2 Months After Calving, Anna Katarzyna Kurpinska, Agnieszka Jarosz, Malgorzata Ozgo, Wieslaw Franciszek Skrzypczak Jan 2016

Analysis Of Protein Expression Changes In The Blood Plasma Of Cows During The Last Month Before Parturition And 2 Months After Calving, Anna Katarzyna Kurpinska, Agnieszka Jarosz, Malgorzata Ozgo, Wieslaw Franciszek Skrzypczak

Turkish Journal of Biology

The organism undergoes tremendous changes at the end of pregnancy and beginning of lactation. The aim of the study was to analyze the physiological changes in cows during the last month of a first pregnancy and the first 2 months of lactation, based on the protein expression in peripheral blood plasma. Changes were observed in the expression of proteins involved in lipid metabolism (apolipoprotein A-I, apolipoprotein E, apolipoprotein J, and apolipoprotein A-IV); immune defense (complement C4 protein and mannose-binding protein C); blood clotting (fibrinogen alpha chain, fibrinogen gamma B-chain, and fibrinogen-like protein 1); as well as reconstruction, regeneration, and defense …

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Amplification Of Gc-Rich Adamts-2 And Urg4/Urgcp Promoter Regions With Optimized Combination Of Pcr Enhancers, Meltem Alper, Esra Tokay, Feray Köçkar Jan 2016

Amplification Of Gc-Rich Adamts-2 And Urg4/Urgcp Promoter Regions With Optimized Combination Of Pcr Enhancers, Meltem Alper, Esra Tokay, Feray Köçkar

Turkish Journal of Biology

PCR is an effective and widely used method for the amplification of target DNA fragments in vitro. The templates that have high guanine-cytosine (GC) content, complex secondary structures, or short tandem repeats are difficult to amplify by the conventional PCR methods. Human genome regulatory regions such as promoters are rich in terms of GC base compositions. To amplify these regions some special modifications or additives are required. Using different PCR strategies such as hot-start/touchdown PCR or the utilization of various additives into the reaction mixture such as organic molecules can improve PCR yield. In the present study we optimized the …

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Cloning And Functional Characterization Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Daiwen Chen, Bing Yu, Junqiu Luo, Zhiqing Huang Jan 2016

Cloning And Functional Characterization Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Daiwen Chen, Bing Yu, Junqiu Luo, Zhiqing Huang

Turkish Journal of Biology

Sox6, a fast myofiber-enriched repressor of slow muscle gene expression, plays an important role in the control of skeletal muscle fiber-type transition. Mammalian Sox6 has been isolated and characterized from mice, rats, and humans. In this study, the porcine Sox6 (pSox6) cDNA was cloned by degenerate PCR. The entire open reading frame (ORF) of pSox6 is 2406 bp. The predicted protein is composed of 801 amino acids and contains the HMG box, leucine-zipper motif, and glutamine-rich Q-box. The amino acid similarities between the Sox6 from pig and other mammals range from 90% to 95%. Real-time quantitative PCR analysis indicated that …

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Identification And Characterisation Of Mlo Genes In Pea (Pisum Sativum L.)Vis-À-Vis Validation Of Mlo Gene-Specific Markers, Chinmayee Mohapatra, Ramesh Chand, Vinay Kumar Singh, Anil Kumar Singh, Chanda Kushwaha Jan 2016

Identification And Characterisation Of Mlo Genes In Pea (Pisum Sativum L.)Vis-À-Vis Validation Of Mlo Gene-Specific Markers, Chinmayee Mohapatra, Ramesh Chand, Vinay Kumar Singh, Anil Kumar Singh, Chanda Kushwaha

Turkish Journal of Biology

Pea is infected by a number of pathogens, and among them powdery mildew (PM) caused by Erysiphe pisi is an important fungal disease. This work was undertaken in silico to characterise the identified PM resistant genes of pea and design specific primers to be used in marker-assisted selection (MAS) of resistant and susceptible pea genotypes. Mlo gene sequences of A. thaliana and O. sativa were retrieved from different databases and used for a homology search of P. sativum EST databases. Each identified gene was used for a similarity search and phylogenetic classification with different crops. Four gene-specific primers were designed …

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Production Of Recombinant Human Dipeptidyl Peptidase Iv From Sf9cells In Microbial Fermenters, Özlem Ayteki̇n, Sai̇me İsmet Gürhan, Kayoko Ohura, Teruko Imai, Gaye Öngen Jan 2016

Production Of Recombinant Human Dipeptidyl Peptidase Iv From Sf9cells In Microbial Fermenters, Özlem Ayteki̇n, Sai̇me İsmet Gürhan, Kayoko Ohura, Teruko Imai, Gaye Öngen

Turkish Journal of Biology

The human dipeptidyl peptidase IV (hDPPIV/CD26) is expressed as an immune response in some cancer cells as well as intestine and incretin metabolism, and deficiency of the enzyme leads to metabolic disorders. In the present study, recombinant hDPPIV/CD26 genes were expressed in baculovirus-insect cell systems in a 5-L stirred-tank fermenter. Because of the shear sensitivity of the insect cell line, production from insect cells should be performed in new-generation type bioreactors, which are commonly more expensive than microbial fermenters. To optimize the process, hydrodynamic parameters and oxygen consumption of Sf9 cells at 1.5 L and 3 L were monitored, and …

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Histopathological And Biochemical Changes In The Liver, Kidney, And Bloodof Amphibians Intoxicated With Cadmium, Marcela Fátima Medina, Maria Elina González, Sandra Maria Roxana Klyver, Iris Maria Aybar Odstrcil Jan 2016

Histopathological And Biochemical Changes In The Liver, Kidney, And Bloodof Amphibians Intoxicated With Cadmium, Marcela Fátima Medina, Maria Elina González, Sandra Maria Roxana Klyver, Iris Maria Aybar Odstrcil

Turkish Journal of Biology

The worldwide decline in amphibian populations has made heavy metal pollution a subject of interest. The aim of this work was to analyze the effect of sublethal doses of cadmium (Cd) on the liver, kidney, and blood of Rhinella arenarum specimens. Serum markers indicative of hepatic injury (gamma glutamyltransferase, alkaline phosphatase, pseudo cholinesterase, and total cholesterol) and markers of renal dysfunction (blood urea nitrogen, serum creatinine, calcium, and glucose) showed variation in animals treated with a 0.5 mg/kg dose compared to controls. Histopathological images revealed alterations in the liver (hepatocyte ballooning and hyperplasia of Kupffer cells) and kidney (renal tubular …

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Phylogenetic Relationships Between Oxytropis Dc. And Astragalus L.Species Native To An Old World Diversity Center Inferred From Nuclear Ribosomal Its And Plastid Matk Gene Sequences, Ayten Tekpinar, Seher Karaman Erkul, Zeki̇ Aytaç, Zeki̇ Kaya Jan 2016

Phylogenetic Relationships Between Oxytropis Dc. And Astragalus L.Species Native To An Old World Diversity Center Inferred From Nuclear Ribosomal Its And Plastid Matk Gene Sequences, Ayten Tekpinar, Seher Karaman Erkul, Zeki̇ Aytaç, Zeki̇ Kaya

Turkish Journal of Biology

Oxytropis and Astragalus represent one of the largest angiosperm genera complexes. Although phylogenetic studies of this complex exist, the evolutionary relationships among Astragalus and Oxytropis species sharing similar habitats in the Old World have not been studied in detail. The phylogenetic relationships among 13 Oxytropis and 56 Astragalus species native to Turkey were inferred from nucleotide sequence variations in the nuclear ribosomal internal transcribed spacer (ITS) and chloroplast maturase-like protein (matK) gene regions. In addition to our samples, 36 Oxytropis ITS and 6 Oxytropis matK sequences were retrieved from GenBank and included in the analysis. Phylogenies derived from a maximum …

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