Digital Commons Network^™

Integration Of Transcriptomic Data With A Genome-Scale Model Reveals Key Metabolic Features Of High Producer Cho Cell Lines, Natalia Jimenez

Cell Culture Engineering XV

No abstract provided.

Understanding And Controlling Sialyation In A Cho Fusion Protein At Lab And Manufacturing Scale Using Targeted Omics Techniques, Amanda Lewis, Nelly Aranibar, William Croughan, Nicholas Abu-Absi, Bethanne Warrack, Michael Borys, Michael Reily, Zheng Jian Li

Cell Culture Engineering XV

Biologics, including antibodies, hormones and cytokines, represent an increasingly important class of therapeutics, with 7 of the 10 top selling drugs from 2013 in this class. The glycosylation distribution of these proteins is an important characteristic that can impact biological activity, circulatory half-life, and immunogenicity. One property that affects glycoproteins is the terminal addition of N-acetylneuraminic acid (sialic acid) to glycosylation chains. Despite the importance of glycosylation in many therapeutic proteins, limited information is available to date linking process parameters to changes in glycosylation distribution. The majority of the work that has been done is limited to a small number …

A Novel Variant In Cmah Is Associated With Blood Type Ab In Ragdoll Cats, Barbara Gandolfi, Robert Grahn, Nicholas Gustafson, Daniela Proverbio, Eva Spada, Badri Adhikari, Janling Cheng, Gordon Andrews, Leslie Lyons, Chris Helps

Computer Science Faculty Works

The enzyme cytidine monophospho-N-acetylneuraminic acid hydroxylase is associated with the production of sialic acids on cat red blood cells. The cat has one major blood group with three serotypes; the most common blood type A being dominant to type B. A third rare blood type is known as AB and has an unclear mode of inheritance. Cat blood type antigens are defined, with N-glycolylneuraminic acid being associated with type A and N-acetylneuraminic acid with type B. Blood type AB is serologically characterized by agglutination using typing reagents directed against both A and B epitopes. While a genetic characterization of blood …

Qualitative And Quantitative Analysis Of Commercial Dna Swabs And Kits, Shelby L. Banks

Honors Theses

With the use of DNA becoming increasingly more important in the field of forensics, the analysis of DNA extraction kits and collection swabs is significant. This researches main objective was to find a protocol that extracts the highest quantity and quality amount of DNA from human buccal swabs. Three extraction kits (Zymo Research Quick – gDNA MiniPrep Capped Column DNA Kit, a Bioline Isolate II Genomic DNA Kit, and Invitrogen PureLink Genomic DNA Kit) were tested with three different swabs (standard cotton swabs, Puritan foam swabs, and Isohelix DNA buccal swabs). Following procedures outline by the DNA kits distributors, DNA …

Rosenthal Uses Ourc Funds For American Chestnut Research, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Unh Manchester Celebrates 264 Students At May 19 Graduation Ceremony, Kim Wall

UNH Today Archive

No abstract provided.

Giving Trees: Protecting Our Urban Forest, Catherine C. Laperche

Student Theses 2015-Present

Over 80% of Americans live in urban areas and that percentage is expected to rise in the coming years. As young people migrate toward cities and the global population rises it is estimated that over 6 billion people will live in urban areas by 2045. The city of the future will not only have to face this population pressure but will also have to deal with rising sea levels, unpredictable weather patterns, increasing energy costs, rising temperatures, and decreasing air quality. Cities that will thrive in this unfavorable future must have strategic plans that will address and alleviate these crucial …

The Gowanus Canal: Delving Into The Murky And Mysterious Waters Of Brooklyn’S Toxic Canal, Victoria L. Von Ancken

Student Theses 2015-Present

Like many environmental issues we face today, the Gowanus Canal began as a hopeful tale. It was envisioned as a promising cargo transportation waterway in Brooklyn, New York. However, due to reckless planning with little thought for the future, the canal slowly began to have a negative effect on its surrounding landscape and community, as well as abandoning its initial purpose, and ultimately sitting stagnant in the neighborhood. Today, the canal is a large environmental problem we face and is in great need of cleanup. The canal is a prime example of a lack of planning and consequential detrimental effects …

A Wasteful Disposition: How A Consumerist Society Can Slowly Become Zero-Waste, Paige G. Werman

Student Theses 2015-Present

Humans have exploited planet earth’s resources to generate many different forms of useless remains: solid waste, food waste, pollution, electronic waste, wastewater, hazardous and chemical waste, and nuclear radioactive waste—so many that we have disregarded the environmental principle that waste in any form is not sustainable or natural. Developed countries have habituated a lifestyle of excess—the societal concept of waste being the most detrimental to the Homo sapiens’ future as a living, thriving species and the reason why we have succumbed to a climate crisis. Through a reframing of ethical philosophies, economic theories, and sustainable design, societies are capable of …

Biological Relevance Of Yap Regulation By Wnt Signaling During Neural Tissue Patterning Of Human Induced Pluripotent Stem Cells, Yan Li

Cell Culture Engineering XV

Human induced pluripotent stem cells (hiPSCs) have special ability to self-assemble into neural microtissues or mini-organ like structures (e.g., mini-brains). In this process, Wnt signaling impacts regional patterning and positional identity of hiPSC-derived neural progenitors. One important function of Wnt signaling is to regulate Yes-associated protein (YAP) expression (nuclear or cytoplasmic), the pivotal regulator of cell proliferation and differentiation during organ growth and tissue generation. However, the crosstalk between Wnt and YAP expression during neural differentiation of hiPSCs has not been well investigated. The objective of this study is to reveal the capability of Wnt signaling in the regulation of …

What Influences Do Age, Gender And Race/Ethnicity Have On The Overall Health Consequences Of Excess Lead In Children?, Jessica J. Smith

Research Papers

After Flint, Michigan governmental authorities introduced a more corrosive water source into an already aging water system without adequate corrosion control, the percentage of children with elevated blood lead levels increased, particularly in socioeconomically disadvantaged neighborhoods. The main objective of this research paper is to clarify influences of age, gender and race, ethnicity have on the overall health consequences of excess lead in children. There are studies that focus on the short and long term effects of neurological, hypertension, renal, and developmental on children. However, since specific demographic evidence is still limited, future research is still needed to implement a …

Not Just Dead, But Gay! Queerness And The Vampire, William A. Tringali

Honors Program Theses and Projects

No abstract provided.

Insights From The Pollination Drop Proteome And The Ovule Transcriptome Of Cephalotaxus At The Time Of Pollination Drop Production, Cary Pirone-Davies, Natalie Prior, Patrick Von Aderkas, Derek Smith, Darryl Hardie, William E. Friedman, Sarah Mathews

Faculty Publications

© The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company. Background and Aims Many gymnosperms produce an ovular secretion, the pollination drop, during reproduction. The drops serve as a landing site for pollen, but also contain a suite of ions and organic compounds, including proteins, that suggests diverse roles for the drop during pollination. Proteins in the drops of species of Chamaecyparis, Juniperus, Taxus, Pseudotsuga, Ephedra and Welwitschia are thought to function in the conversion of sugars, defence against pathogens, and pollen growth and development. To better understand gymnosperm pollination biology, the pollination …

An Analysis Of Canine Processing Of Stimulus Compounds Varying In Light And Sound Intensity, Katherine O. Compitus

Theses and Dissertations

A dog was trained to respond deferentially to two light-sound compounds. The dog was then tested with combinations of additional light and sound intensities. The dog appeared to use the information provided by both stimulus dimensions. This research is relevant to the understanding of information processing, specifically categorization and generalization.

High Salt Induces Anti-Inflammatory Mφ2-Like Phenotype In Peripheral Macrophages, Suneetha Amara, Margaret M. Whalen, Venkataswarup Tiriveedhi

Chemistry Faculty Research

Macrophages play a critical role in inflammation and antigen-presentation. Abnormal macrophage function has been attributed in autoimmune diseases and cancer progression. Recent evidence suggests that high salt tissue micro-environment causes changes in macrophage activation. In our current report, we studied the role of extracellular sodium chloride on phenotype changes in peripheral circulating monocyte/macrophages collected from healthy donors. High salt (0.2M NaCl vs basal 0.1M NaCl) treatment resulted in a decrease in MΦ1 macrophage phenotype (CD11b+CD14highCD16low) from 77.4±6.2% (0.1M) to 29.3±5.7% (0.2M, p<0.05), while there was an increase in MΦ2 macrophage phenotype (CD11b+ CD14lowCD16high) from 17.2±5.9% (0.1M) to 67.4±9.4% (0.2M, p<0.05). ELISA-based cytokine analysis demonstrated that high salt treatment induced decreased expression of in the MΦ1 phenotype specific pro-inflammatory cytokine, TNFα (3.3 fold), IL-12 (2.3 fold), CCL-10 (2 fold) and CCL-5 (3.8 fold), but conversely induced an enhanced expression MΦ2-like phenotype specific anti-inflammatory cytokine, IL-10, TGFβ, CCL-17 (3.7 fold) and CCR-2 (4.3 fold). Further high salt treatment significantly decreased phagocytic efficiency of macrophages and inducible nitric oxide synthetase expression. Taken together, these data suggest that high salt extracellular environment induces an anti-inflammatory MΦ2-like macrophage phenotype with poor phagocytic and potentially reduced antigen presentation capacity commonly found in tumor microenvironment.

Utilizing The Next Generation Science Standards As A Framework To Create A Climate Change Curriculum, Jenna Totz

School of Education and Leadership Student Capstone Theses and Dissertations

The research question addressed in this project was, how can the Next Generation Science Standards be utilized as a framework to create a climate change curriculum? It documents the creation of the middle school climate change curriculum, Next Generation Climate. The curriculum integrates experiential education, argumentation, and modeling all using the most current climate change science. This curriculum was created out of the need for more resources that aligned with the NGSS and to provide educators with the tools to correctly provide climate literacy to their students. The author documents the details of writing the curriculum and uses research literature …

Uno Math Science Learning Center Program Of Excellence Report 2015-16, College Of Arts And Sciences, Math Science Learning Center

Student Support

Since its opening in the Fall of 2007, the UNO Math-Science Learning Center has 1) provided peer tutoring for 1000’s of UNO students studying math and science, 2) organized/offered a variety of STEM workshops and outreach events and 3) collaborated with STEM faculty across campus on a variety of grants, outreach, strategic planning and other activities.

Eba Playful Experiences, Lara Cardoso Goulart

Theses

This thesis is a study of the impact of busy lifestyles on children’s education, considering playtime. Eba Playful Experiences is the result of applied design methodology to solve the lack of play between children and parents, taking into account their routine and needs.

The research includes the study of the importance of play and the problems caused by its absence. The process contains user understanding considering children’s stages of physical, social and cognitive development. The project involves learning from different areas of study: product and interaction design, child psychology and business.

Eba Playful Experiences is an online company based on …

Biotherapeutic Development In The 'Omics Age: The Cho Genome And Beyond, Chapman Wright, Joost Groot, Scott Estes

Cell Culture Engineering XV

The Next-Generation Sequencing (NGS) revolution has had a profound effect on the manner in which we approach the fields of biology and medicine, but the effects are not limited to these fields alone. With the process of collecting and analyzing large datasets becoming more seamless, a growing number of disciplines are incorporating NGS and other ‘Omics technologies into their workflow. Biotherapeutic development is one such field that is benefitting from the comprehensive and rich datasets that NGS can provide. At Biogen, we are using NGS in two ways: to provide a deeper understanding CHO cell biology during bioprocessing and to …

Agitation Strategies For The Culture And Detachment Of Human Mesenchymal Stem Cells (Hmscs) From Microcarriers In Multiple Bioreactor Platforms, Alvin Nienow

Cell Culture Engineering XV

Unlike cell culture for biopharmaceuticals, where the product of interest is usually a recombinant protein, for regenerative medicine, the cells form the basis of the therapeutic. For the expansion of cells for allogeneic purposes, work, mainly in spinner flasks, has led to culture on microcarriers. For processes involving mass transfer and reaction with particles in stirred reactors (e.g., crystallization, catalytic reaction), it is essential that particles are at least just fully suspended, agitator speed N_JS, for effective mass transfer to and from the particles. Though gentle agitation has generally been recommended, it has not previously been defined in …

Development Of A Process Analytical Technology (Pat) Infrastructure For Future Biologics Upstream Processing, Mathieu Steefland

Cell Culture Engineering XV

Process Analytical Technology (PAT) involves the timely analysis of Critical Quality Attributes (CQAs) and Critical Process Parameters (CPPs) with the aim to improve the process control strategy resulting in a better process performance and robust product quality. The progress of PAT implementation in upstream bioprocessing has been slow mainly due to the complexity of the process steps with many parameters involved, and the complexity of the product and the culture medium. While the first approved PAT-based Real Time Release application in the small molecules field already celebrating its 10th anniversary in 2016, PAT for upstream bioprocessing is only now maturing. …

Cell-Derived Microparticles For Cell Therapy, Cargo Delivery, And Applications In Cho-Cell Biotechnology, Terry Papoutsakis, Jinlin Jiang, Chen-Yuan Kao

Cell Culture Engineering XV

Mammalian cells release into the extracellular environment microparticles (MPs; less than 1 micron) under some stress or activation process. MPs result from direct budding off the plasma membrane, and are important in intercellular communication by transferring RNA, proteins, and lipids between cells. Cells endow their MPs with signaling or functional molecules, to target specific cell types. They enrich their MPs in specific miRNAs, piRNAs, and long ncRNAs to program or reprogram target cells towards functional differentiation or specific cellular actions. Cells also use MPs to get rid of “death molecules”, and/or promote cell survival and “renewal” of target cells. We …

Development Of A Course-Based Undergraduate Research Experience To Introduce Drug-Receptor Concepts, Hollie I. Swanson, Ok-Kyong Park-Sarge, Thushani Rodrigo-Peiris, Lin Xiang, Vincent M. Cassone

Pharmacology and Nutritional Sciences Faculty Publications

Course-based research experiences (CUREs) are currently of high interest due to their potential for engaging undergraduate students in authentic research and maintaining their interest in science, technology, engineering, and mathematics (STEM) majors. As part of a campuswide initiative called STEMCats, which is a living learning program offered to freshman STEM majors at the University of Kentucky funded by a grant from Howard Hughes Medical Institute, we have developed a CURE for freshmen interested in pursuing health care careers. Our course, entitled “Drug–Drug Interactions in Breast Cancer,” utilized a semester-long, in-class authentic research project and instructor-led discussions to engage students in …

Slaying Hybridity: The “Uncreated” Monstrous Doubles In The The Saga Of The Volsungs, Nibelungenlied, And Kudrun, Kyle Augustyniak

Seton Hall University Dissertations and Theses (ETDs)

This paper examines anxieties about gender and gender in a thirteenth-century Old Norse text, The Saga of the Volsungs, and two Middle High German texts, the Nibelungenlied and Kudrun. All three exhibit a similar uneasiness about gender performance. In these epics, fears about gender fluidity are founded on the slaying of hybrid mythological creatures (dragons and griffons) and are perpetuated by various media: blood, tears, clothing, promises, and lies. These monsters threaten the established order within the text, a patriarchal order defined by feudal society. Once the hero defeats the monster—whose very essence is simultaneously an embodiment of the perceived …

Immune Function In Amazonian Horticulturalists, Aaron D. Blackwell, Benjamin C. Trumble, Ivan Maldonado Suarez, Jonathan Stieglitz, Bret Beheim, J. Josh Snodgrass, Hillard Kaplan, Michael Gurven

ESI Publications

Background—Amazonian populations are exposed to diverse parasites and pathogens, including protozoal, bacterial, fungal, and helminthic infections. Yet much of our understanding of the immune system is based on industrialised populations where these infections are relatively rare.

Aim—We examine distributions and age-related differences in 22 measures of immune function for Bolivian forager-horticulturalists and US and European populations.

Subjects and Methods—Subjects were 6,338 Tsimane aged 0–90 years. Blood samples collected between 2004–2014 were analysed for 5-part blood differentials, C-reactive protein, erythrocyte sedimentation rate (ESR), and total immunoglobulins E, G, A, and M. Flow cytometry was used to quantify naive …

Signaling In Effector Lymphocytes: Insights Toward Safer Immunotherapy, Kamalakannan Rajasekaran, Matthew J. Riese, Sridhar Rao, Li Wang, Monica Thakar, Charles Sentman, Subramaniam Malarkannan

Dartmouth Scholarship

Receptors on T and NK cells systematically propagate highly complex signaling cascades that direct immune effector functions, leading to protective immunity. While extensive studies have delineated hundreds of signaling events that take place upon receptor engagement, the precise molecular mechanism that differentially regulates the induction or repression of a unique effector function is yet to be fully defined. Such knowledge can potentiate the tailoring of signal transductions and transform cancer immunotherapies. Targeted manipulations of signaling cascades can augment one effector function such as antitumor cytotoxicity while contain the overt generation of pro-inflammatory cytokines that contribute to treatment-related toxicity such as …

Spartan Daily, May 12, 2016, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2016

Volume 146, Issue 42

The Pacifican May 12, 2016, University Of The Pacific

All Issues - Student Newspaper, The Pacifican, Pacific Weekly

No abstract provided.

Filter Feeding, Sam Boven

Graduate School of Art Theses

This paper is an investigation of the mechanics of evolutionary processes and its methods. These investigations are representative of both a world view and a methodology of artistic production. I consider a central consideration of my current work to be the interaction of distinct components and the new experience that these interactions produce when pondered by a viewer. Evolution and the development of life is driven ever forward by the interactions between organisms, both as groups and individuals. By grouping these relationships into three categories: parasitism, predation, and symbiosis, I aim to provide a lens through which to consider both …

Fundamental Awareness: A Framework For Integrating Science, Philosophy And Metaphysics, Neil D. Theise, Menas Kafatos

Mathematics, Physics, and Computer Science Faculty Articles and Research

The ontologic framework of Fundamental Awareness proposed here assumes that non-dual Awareness is foundational to the universe, not arising from the interactions or structures of higher level phenomena. The framework allows comparison and integration of views from the three investigative domains concerned with understanding the nature of consciousness: science, philosophy, and metaphysics. In this framework, Awareness is the underlying reality, not reducible to anything else. Awareness and existence are the same. As such, the universe is non-material, self-organizing throughout, a holarchy of complementary, process driven, recursive interactions. The universe is both its own first observer and subject. Considering the world …