Digital Commons Network^™

Depth Profiles Of Persistent Organic Pollutants In The North And Tropical Atlantic Ocean, Caoxin Sun, Thomas Soltwedel, Eduard Bauerfeind, Dave A. Adelman, Rainer Lohmann

Graduate School of Oceanography Faculty Publications

Little is known of the distribution of persistent organic pollutants (POPs) in the deep ocean. Polyethylene passive samplers were used to detect the vertical distribution of truly dissolved POPs at two sites in the Atlantic Ocean. Samplers were deployed at five depths covering 26–2535 m in the northern Atlantic and Tropical Atlantic, in approximately one year deployments. Samplers of different thickness were used to determine the state of equilibrium POPs reached in the passive samplers. Concentrations of POPs detected in the North Atlantic near the surface (e.g., sum of 14 polychlorinated biphenyls, PCBs: 0.84 pg L^–1) were similar …

An Exploration Of Adolescent Obesity Determinants, Anastasia King Smith

USF Tampa Graduate Theses and Dissertations

In 2010, approximately two-thirds of adults and one-fifth of the adolescent population in the United States were considered either overweight or obese, resulting in the United States having the highest per capita obesity rate among all OECD countries. A considerable body of literature regarding health behavior, health outcomes, and public policy exists on what the Centers for Disease Control and Prevention considers an obesity epidemic. In response to the growing problem of childhood obesity, the Child Nutrition and WIC Reauthorization Act of 2004 (CNRA), which required that schools participating in the National School Lunch Program and/or School Breakfast Program have …

Ocean Acidification Accelerates The Growth Of Two Bloom-Forming Macroalgae, Craig S. Young, Christopher J. Gobler

School of Marine & Atmospheric Sciences Faculty Publications

While there is growing interest in understanding how marine life will respond to future ocean acidification, many coastal ecosystems currently experience intense acidification in response to upwelling, eutrophication, or riverine discharge. Such acidification can be inhibitory to calcifying animals, but less is known regarding how non-calcifying macroalgae may respond to elevated CO2. Here, we report on experiments performed during summer through fall with North Atlantic populations of Gracilaria and Ulva that were grown in situ within a mesotrophic estuary (Shinnecock Bay, NY, USA) or exposed to normal and elevated, but environmentally realistic, levels of pCO2 and/or nutrients (nitrogen and phosphorus). …

The Murray Ledger And Times, May 13, 2016, The Murray Ledger And Times

The Murray Ledger & Times

No abstract provided.

Differential Styles Of Emotional Reactivity And Antisocial Behavior Relative To Post-Traumatic Stress Disorder Symptom Expression In Detained Youth, Molly A. Miller

LSU New Orleans Theses and Dissertations

This study sought to explore whether PTSD symptoms in detained adolescents were differentially related to high and low patterns of emotional reactivity, as determined based on callous-unemotional (CU) traits and emotion dysregulation. Analyses revealed four distinct groups based on these criteria: a low/no trauma control group with few PTSD symptoms, and three groups whose PTSD symptoms were distinguished by symptoms of emotional numbing. The study sought to determine whether these profiles were related to distinct patterns of aggression and delinquency. Results revealed that the low/no PTSD symptom group exhibited the least aggression and delinquency. Further, results indicated that higher CU …

Newsroom, Georgia Southern University

Newsroom (2006-2023)

• Georgia Southern University receives two Emmy Award nominations
• Daniel J. Cox, Ph.D., to serve as founding chair of Department of Manufacturing Engineering
• Threatre and Performance Program receives Full Membership in NAST
• Seventh annual Farm-to-Table Dinner fundraiser to support Henderson Library

Survey Of Autonomic Computing And Experiments On Jmx-Based Autonomic Features, Adel R. Azzam

LSU New Orleans Theses and Dissertations

Autonomic Computing (AC) aims at solving the problem of managing the rapidly-growing complexity of Information Technology systems, by creating self-managing systems. In this thesis, we have surveyed the progress of the AC field, and studied the requirements, models and architectures of AC. The commonly recognized AC requirements are four properties - self-configuring, self-healing, self-optimizing, and self-protecting. The recommended software architecture is the MAPE-K model containing four modules, namely - monitor, analyze, plan and execute, as well as the knowledge repository.

In the modern software marketplace, Java Management Extensions (JMX) has facilitated one function of the AC requirements - monitoring. Using …

The Digital Badge Initiative And Its Implications For First-Year Writing, Rena Tillinghast

LSU New Orleans Theses and Dissertations

College students seek degrees to obtain employment in their field of interest, however, as the 21st century progresses, employers are often requiring specific skills in addition to degrees and transcripts. As students graduate with their Associates, Bachelors, and Graduate degrees, they plan to present these degrees as sufficient evidence of their qualifications. However, there is recent criticism of college degrees as evidence of qualifications. A beneficial alternative for students would be digital badges. A digital badge is a visual representation that signifies a specific achievement with detailed metadata attached. Digital badges in first-year writing courses would benefit students as they …

Figure-Ground Organization Using 3d Symmetry, Aaron Michaux, Vijai Jayadevan, Edward Delp, Zygmunt Pizlo

MODVIS Workshop

We present a novel approach to object localization using mirror symmetry as a general purpose and biologically motivated prior. 3D symmetry leads to good segmentation because (i) almost all objects exhibit symmetry, and (ii) configurations of objects are not likely to be symmetric unless they share some additional relationship. Furthermore, psychophysical evidence suggests that the human vision system makes use symmetry in constructing 3D percepts, indicating that symmetry may be important in object localization. No general purpose approach is known for solving 3D symmetry correspondence in 2D camera images, because few invariants exist. Therefore, to test symmetry as a clustering …

Writing A Canadian High School History Of The Great War: Victoria High School: Challenges, Pitfalls, And Sources, Barry Gough

Canadian Military History

Contained in this article are suggestions on how to write a history of a Canadian high school and the First World War. Included in the discussion is the approach and methodology of the historian, the materials available for use, and knowledge of the background of Canada, the British Empire and the war “for King and Country.” It appeals for an understanding of war and of patriotism Canadian-style as of 1914-1918, as a war they fought and not the one we now think they fought or should have fought. It is an appeal for “sharp end” history. Attention is given to …

Assessment Of Soil Washing For Simultaneous Removal Of Heavy Metals And Low-Level Petroleum Hydrocarbons Using Various Washing Solutions, Deok-Hyun Moon, Jae-Woo Park, Agamemnon Koutsospyros, Kyung Hoon Cheong, Yoon-Young Chang, Ki-Tae Baek, Raehyun Jo, Jeong-Hun Park

Civil Engineering Faculty Publications

Bench-scale soil washing experiments were conducted for simultaneous removal of heavy metals (Pb, Cu, Zn) and low-level petroleum hydrocarbon contaminants from soils. Various washing solutions including hydrochloric acid (HCl), nitric acid (HNO₃), sulfuric acid (H₂SO₄), tartaric acid (C₄H₆O₆) and ethylenediaminetetraacetic acid (C₁₀H₁₆N₂O₈, EDTA) were used. The concentration of the washing solutions ranged from 0.1 to 3M with a liquid-to-solid ratio of 10. The soil washing results showed that hydrochloric acid (HCl) was the best washing solution at 3M for heavy …

2016-01-A3dsrinp-Csc-Sta-Cmb-522-Bps-542, Raymond Pulver, Neal Buxton, Xiaodong Wang, John Lucci, Jean Yves Hervé, Lenore Martin

Bioinformatics Software Design Projects

Cholesterol is carried and transported through bloodstream by lipoproteins. There are two types of lipoproteins: low density lipoprotein, or LDL, and high density lipoprotein, or HDL. LDL cholesterol is considered “bad” cholesterol because it can form plaque and hard deposit leading to arteries clog and make them less flexible. Heart attack or stroke will happen if the hard deposit blocks a narrowed artery. HDL cholesterol helps to remove LDL from the artery back to the liver.

Traditionally, particle counts of LDL and HDL plays an important role to understanding and prediction of heart disease risk. But recently research suggested that …

Western News, May 12, 2016, Western Michigan University

Western News (1972-2018)

Story highlights: Story highlights: WMU recognized as Purple Heart University; Koretsky tapped to lead College of Arts and Sciences; Annual fall convocation to feature new format, location; On Campus with Dillon Dolby.

Next Gen Car T Cells, Michael Jensen

Cell Culture Engineering XV

Recent conceptual as well as technological advances in the areas of molecular immunology, gene transfer, and cell processing have fostered increasingly sophisticated translational applications of adoptive T cell therapy for oncologic disease employing genetically-modified T-lymphocytes. My laboratory’s work focuses on T-cell genetic modification for re-directing antigen specificity to tumors utilizing recent advances not only in the composition and specificity of receptor antigen recognition domains, but also the evolution of multifunctional cytoplasmic signaling domains developed for these chimeric antigen receptors (CARs) that provide dual activation and co-stimulatory signaling. My group is also investigating the context of adoptive transfer with respect to …

A Bioinformatic Pipeline For Studying Ribosome Occupancy In Cho Cells, Shangzhong Li

Cell Culture Engineering XV

No abstract provided.

Increasing Diversity Of Production Cell Lines Through Miniaturization, Automation, And High-Throughput Analytics, Kim Le, Hannah Victor, Kristi Daris, Trent Munro

Cell Culture Engineering XV

The development of a successful biologic therapeutic manufacturing process begins with the creation of a stable clonal cell line. Since attributes of the production cell line will significantly impact upstream and downstream processes, researchers must find ways to generate several candidate lines with diverse properties. However, a wide diversity is difficult to achieve since cultures are commonly selected, maintained, and screened as populations. In these populations, robust sub-populations can overtake the overall culture and reduce diversity. To combat this, sub-populations must be physically separated by splitting or subcloning, and maintained in individual vessels requiring intensive labor and infrastructure. As a …

Targeting Product Quality: Where Systems Biotechnology And Process Design Meet, Markus Michael Muller, Jan Bechmann, Ingo Gorr, Harald Bradl, Jan Visser

Cell Culture Engineering XV

Product quality is a result of the entire production process including protein sequence, host cell, media and process parameters. Many of the desired product properties are defined by posttranslational modifications with impact on biological activity, immunogenicity, half-life or stability. In‑depth understanding of the host cells capabilities as well as of the process interactions enables the targeted modulation of product quality attributes by rational selection of host cells and design of bioprocesses. This is valuable for new biological molecules in order to improve efficacy, reduce side effects, access new patient populations. For biosimilars this allows developing into defined quality attribute profiles. …

A Community Genome-Scale Model Of Chinese Hamster Ovary Cell Metabolism Identifies Differences In The Efficiency Of Resource Utilization For Various Bioprocesses, Hooman Hefzi

Cell Culture Engineering XV

Genome-scale models of metabolism have successfully been employed in many microbial and eukaryotic metabolic engineering efforts by guiding pathway engineering and media optimization. They have also been used to explore the genotype-phenotype relationship in mammalian cells. The publication of the genomic sequence for Chinese hamster ovary (CHO) cells has allowed generation of genome-scale metabolic models (GeMs) for this organism. Here we have developed a high-quality community CHO GeM via careful reconciliation and manual curation of three independently developed CHO GeMs. This metabolic model, consisting of over 4000 metabolites and 6000 reactions, is capable of integrating proteomic, transcriptomic, and metabolomic data …

Human Cardiac Stem Cells For Allogeneic Cell Therapies: Integrating Bioprocess Development And ‘Omics Characterization Tools, Patricia Gomes

Cell Culture Engineering XV

Cardiovascular diseases (CVDs) are the leading cause of death worldwide. Myocardial infarction (AMI) severely affects patients’ heart muscle and microvasculature, critically decreasing the number of functional cardiomyocytes (CM). Stem cell and protein based therapies became promising cardiac repair strategies since it was found that under pathological stress, resident cardiac stem cells (CSC) of the adult myocardium are activated by growth factors (GF) secreted by the surviving CM. Consequently, an auto/paracrine loop is triggered to maintain GF production, which enhances CSC activation and differentiation into new CM, endothelial and smooth muscle cells contributing to the repair of damaged myocardium [1]. Since …

Scale-Down And Initial Characterization Studies Of An Allogeneic Cell Therapy Manufacturing Process, Thomas Brieva

Cell Culture Engineering XV

Cancer therapies leveraging the immune system have been improving the course of disease. T-cell therapies are one of the modalities of immune therapy, which also include small molecules, proteins, and various types of immune cells. T-cell therapies include T-cell Receptor cells (TCR), Chimeric Antigen Receptor (CAR) T-cells, and Cytotoxic T-Lymphocytes (CTL). Although the nascent cell therapy industry faces many manufacturing challenges, it benefits from the vast experience of the blood industry and cell-produced biologics industry.

A notable manufacturing challenge for most T-cell therapies results from their autologous nature, in which cells from a patient are used to produce the therapeutic …

Multi-Omic Profiling Of Epo Producing Cho Cell Panel Reveals Metabolic Adaptation To Heterologous Protein Production, Daniel Ley

Cell Culture Engineering XV

The Chinese hamster ovary (CHO) cell line is the predominant mammalian cell factory for production of therapeutic glycoproteins. In this work, we aimed to study bottlenecks in the secretory pathway associated with the production of human erythropoietin (EPO) in CHO cells. In connection to this, we discovered indications of metabolic adaptation of the amino acid catabolism in favor of heterologous protein production. We established a panel of stably EPO expressing CHO-K1 clones spanning a 25-fold productivity range and characterized the clones in batch and chemostat cultures. For this, we employed a multi-­omic physiological characterization including metabolic footprinting of amino acids, …

Michael Borys (Bristol- Myers Squibb) Incorporation Of Qbd Elements Into The Development And Characterization Of A Second Generation Process, Michael Borys, Nicholas Abu-Absi

Cell Culture Engineering XV

QbD principles are readily incorporated into mammalian cell processes to streamline process development and characterization. A key enabler of the implementation of these principles has been widespread adoption of platform technologies by the industry. This allows easy and efficient navigation of the QbD roadmap laid out in the A-Mab case study over the course of the development lifecycle of a product.

Here we examine the case of a 2nd generation process for a legacy product that was originally developed and approved using the traditional approach to process development and characterization. The goal of the 2nd generation process was to achieve …

Manipulation And Exploitation Of Micrornas For Enhanced Recombinant Protein Production In Cho Cells, Tulshi Patel, Lyne Josse, Mark Smales, Robert Young

Cell Culture Engineering XV

MicroRNAs (miRNAs) are regulatory non-coding RNA molecules around 22 nucleotides in length which control gene expression via translational repression of their cognate messenger RNA(s) (mRNAs). Regulating as much as 30% of the genome, miRNAs exhibit a high level of promiscuity as a single miRNA is able to target multiple mRNA targets and a single mRNA transcript can be under the regulation of several miRNAs. Thus, global regulation and control of almost all cellular processes and pathways is heavily dependent on the level and activity of miRNAs. This work has set out to investigate the biology of miRNAs in the industrially …

Fluorescent Influenza-Like Particles And Control Over Their Composition, Marc Aucoin

Cell Culture Engineering XV

The Baculovirus Expression Vector System (BEVS) is a proven platform for making biologics. Despite being widely used, the system has typically relied on ‘brute force’ high expression. Building off of the handful of scientific publications that have shown that alternate promoter usage can promote higher functional protein yields, we have sought to generate baculovirus constructs that make use of a greater variety of promoters and genetic elements (shRNA/CRISPRi) to better control gene expression.

Materials and Methods

Genes for green fluorescent protein (GFP), DsRed2, and iRFP682, alone or as fusions to the human influenza A/PR/8/34 hemagglutinin (HA), matrix (M1) and neuraminidase …

Understanding And Overcoming Process Insults Through Application Of ‘Omics Technologies, Alan Gilbert, Kyle Mcelearney, Smitha Krishnan, Nicholas Alden, William Yang, Kyongbum Lee, Rashmi Kshirsagar

Cell Culture Engineering XV

Modern industrial process development, at both small and large corporations, usually consists of applying a well-characterized and established cell culture platform. Despite the high productivity available from these process platforms, difficult challenges remain, including with respect to the ability of the process to endure insults or disruptions. We previously demonstrated that overfeeding resulted in an undesirable increase in lactate production late in fed batch culture, which decreased productivity[i]. Here we report on metabolic flux analysis performed utilizing this process and isotopically labeling with multiple tracers (glucose and glutamate) delivered at five distinct time points of the cell culture …

Benthic Algae Compensate For Phytoplankton Losses In Large Aquatic Ecosystems, Soren Brothers, Yvonne Vadeboncoeur, Paul Sibley

Watershed Sciences Faculty Publications

Anthropogenic activities can induce major trophic shifts in aquatic systems, yet we have an incomplete understanding of the implication of such shifts on ecosystem function and on primary production (PP) in particular. In recent decades, phytoplankton biomass and production in the Laurentian Great Lakes have declined in response to reduced nutrient concentrations and invasive mussels. However, the increases in water clarity associated with declines in phytoplankton may have positive effects on benthic PP at the ecosystem scale. Have these lakes experienced oligotrophication (a reduction of algal production), or simply a shift in autotrophic structure with no net decline in PP? …

Otter Realm, May 12, 2016, California State University, Monterey Bay

The Otter Realm

Done With the B.S.: $upporting $cholars -- Illustrating the future of science: Graduate students design textbook illustrations -- Stick up for a change -- Funding the future: Seaside Scholars -- Getting the best shot of Cuba's LGBTQ Pride -- Promoting students' health & wellness -- Committed to the cause: President's Award recipients -- Alumni Vision Award -- Social Justice Award for awareness of HCV -- Fun in the Sun -- Do you mind? Exploring the quality of mental health services at CSUMB -- Growing up, are we ready? -- General Election 2016: Which Candidate will you support? -- Sex on …

Pain And Laboratory Animals: Publication Practices For Better Data Reproducibility And Better Animal Welfare, Larry Carbone, Jamie Austin

Laboratory Research and Animal Welfare Collection

Scientists who perform major survival surgery on laboratory animals face a dual welfare and methodological challenge: how to choose surgical anesthetics and post-operative analgesics that will best control animal suffering, knowing that both pain and the drugs that manage pain can all affect research outcomes. Scientists who publish full descriptions of animal procedures allow critical and systematic reviews of data, demonstrate their adherence to animal welfare norms, and guide other scientists on how to conduct their own studies in the field. We investigated what information on animal pain management a reasonably diligent scientist might find in planning for a successful …

Toward Development Of Continuous Bioprocesses: Comparison Of Fed-Batch And Perfusion Upstream Production Processes In Early Development, Jean Mclarty, Daryl Powers, Christine Hamel, Betsy Simons, Ken Karey

Cell Culture Engineering XV

Continuous Processing is an exciting development in the field of bioprocessing. The potential for quick response to market demands, decrease in infrastructure, increased flexibility and consistent product quality has resulted in a growing interest in Continuous Processing for production of all types of protein drugs (high or low volume, stable or unstable). Sanofi is developing a novel Integrated Continuous Manufacturing platform for biologics that utilizes an upstream perfusion process. While cell culture perfusion processes offer substantial benefits for commercial biologics production, implementation may present challenges in early development, where speed to first in man studies is critical. Here we present …

Improving Biologics Development By High Performance Glycoanalysis, Erdmann Rapp

Cell Culture Engineering XV

Glycomics is a rapidly emerging field that can be viewed as a complement to other „omics“ approaches including proteomics and genomics. Hence, there is a dramatic increase in the demand for analytical tools and specific databases in glycobiology, respectively, glycobiotechnology. In order to enhance and improve the comparatively small existing glycoanalytical toolbox, fully automated, highly sensitive, reliable, high-throughput and high-resolution analysis methods including automated data evaluation are required.

One very promising method is based on multiplexed capillary gelelectrophoresis with laser induced fluorescence detection (xCGE-LIF). The glycoanalytical approach established includes sample preparation and measuring methods, software, and database solutions to tackle …