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2016

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Linking Transcriptional And Physiological Responses To High Light Stress In A Wild-Type And A Photomorphogenic Tomato Mutant, Jordyn Radke Nov 2016

Linking Transcriptional And Physiological Responses To High Light Stress In A Wild-Type And A Photomorphogenic Tomato Mutant, Jordyn Radke

Theses and Dissertations

Tomato (Solanum lycopersicon) is the leading vegetable crop worldwide and an important part of a healthy human diet. In this study we used Next-Generation Sequencing, metabolomics and gene ontologies to study the effects of high light stress on the photomorphogenic hp-2dg tomato mutant. Wild-type (cv. ‘Manapal’) and mutant plants were grown for six weeks under low light and transferred to high light for one week. Illumina-based RNA Sequencing was performed and analysis of differential gene expression identified over 650 transcripts that were strongly regulated by light intensity (FC>4, p2,p<0,01). HPLC analysis of tomato leaves showed differential accumulation of phenylpropanoid metabolites such as chlorogenic acid, rutin, and caffeoylglucaric acid which was strongly dependent on genotype and light condition. Our findings show insightful differences between the wildtype and the photomorphogenic mutant as well as novel genes involved in high light adaptation in plants.


An Evaluation Of Voluntary Pre-Kindergarten (Vpk) Participation On Third Grade Reading And Math Performance In A Rural School System, Christy Deberry Smith Nov 2016

An Evaluation Of Voluntary Pre-Kindergarten (Vpk) Participation On Third Grade Reading And Math Performance In A Rural School System, Christy Deberry Smith

Electronic Theses and Dissertations Archive

This study examined the longitudinal performance of a district-wide cohort of students who had accumulated scores for both kindergarten and third grade analysis. The sample was disaggregated by Pre-kindergarten participation and student demographics such as gender, age, minority status, rural class, and socio-economic status. STAREarly Literacy, STARReading, and STARMath assessment scores were used for the data analysis. Sample data was collected from the beginning of kindergarten and at the end of third grade.Two types of analysis were conducted to complete the study. This study investigated the analysis of student performance measures based on participation in a Pre-kIndergarten program as well …


Secondary Analysis Of Morbidity Data From A Rural Haitian Village., Emma Karin Draluck Nov 2016

Secondary Analysis Of Morbidity Data From A Rural Haitian Village., Emma Karin Draluck

Electronic Theses and Dissertations Archive

Haiti is the poorest country in the Western Hemisphere with most of the population surviving on $1 per day. Health outcomes among Haitians are poor, with a life expectancy 16 years less than the US. The majority of health research in Haiti has been narrow, focusing on specific issues like tuberculosis, HIV/AIDS, maternal health, and cholera. Unfortunately, this has resulted an incomplete understanding of health in Haiti. This study aims to fill this gap by describing the most common health complaints, how they differ by age, sex, and temporality, and if there are associations between these health issues and environmental …


November 28, 2016, The Daily Mississippian Nov 2016

November 28, 2016, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.


Problems Of Multi-Species Organisms: Endosymbionts To Holobionts, David C. Queller, Joan E. Strassmann Nov 2016

Problems Of Multi-Species Organisms: Endosymbionts To Holobionts, David C. Queller, Joan E. Strassmann

Biology Faculty Research

The organism is one of the fundamental concepts of biology and has been at the center of many discussions about biological individuality, yet what exactly it is can be confusing. The definition that we find generally useful is that an organism is a unit in which all the subunits have evolved to be highly cooperative, with very little conflict. We focus on how often organisms evolve from two or more formerly independent organisms. Two canonical transitions of this type—replicators clustered in cells and endosymbiotic organelles within host cells—demonstrate the reality of this kind of evolutionary transition and suggest conditions that …


Effect Of Electrolyzed High-Ph Alkaline Water On Blood Viscosity In Healthy Adults., Joseph J. Weidman, Ralph E. Holsworth, Bradley Brossman, Daniel J. Cho, John St Cyr, Gregory Fridman Nov 2016

Effect Of Electrolyzed High-Ph Alkaline Water On Blood Viscosity In Healthy Adults., Joseph J. Weidman, Ralph E. Holsworth, Bradley Brossman, Daniel J. Cho, John St Cyr, Gregory Fridman

Student Papers, Posters & Projects

BACKGROUND: Previous research has shown fluid replacement beverages ingested after exercise can affect hydration biomarkers. No specific hydration marker is universally accepted as an ideal rehydration parameter following strenuous exercise. Currently, changes in body mass are used as a parameter during post-exercise hydration. Additional parameters are needed to fully appreciate and better understand rehydration following strenuous exercise. This randomized, double-blind, parallel-arm trial assessed the effect of high-pH water on four biomarkers after exercise-induced dehydration.

METHODS: One hundred healthy adults (50 M/50 F, 31 ± 6 years of age) were enrolled at a single clinical research center in Camden, NJ and …


Chhs News, College Of Health And Human Sciences, Georgia Southern University Nov 2016

Chhs News, College Of Health And Human Sciences, Georgia Southern University

College of Health Professions: News & Publications (2012-2023)

No abstract provided.


The Effect Of Teaching Strategies And Learning Environment On Certification Exam Pass Rates In Dietetic Education, Debra Head Nov 2016

The Effect Of Teaching Strategies And Learning Environment On Certification Exam Pass Rates In Dietetic Education, Debra Head

Theses and Dissertations

The obligation for preparing competent graduates begins with an educational experience in an environment that promotes optimal learning. An important outcome of successful health professions education is the passage of the certification exam. The objective of this study was to evaluate learning outcomes and teaching strategies and the effect on certification exam pass rate in dietetic education programs. A non-experimental associational cross-sectional mixed methods design was chosen for the study in order to gather objective data through the use of the previously validated Constructivist Learning Environment Survey. A 30 item electronic survey was administered to faculty in dietetic education programs …


Health & Kinesiology News, Georgia Southern University Nov 2016

Health & Kinesiology News, Georgia Southern University

Health Sciences & Kinesiology: News & Publications (2015-2023)

  • Faculty and Students Participate in 2016 Southeast Farm to School Conference


Supercritical Co2 Extraction Of Idesia Polycarpa Oil And Analysis Of Its Fatty Acid Composition, Kuang Chuntao, Wu Bin, Tang Hongwei, Xiang Shu, He Jianyang Nov 2016

Supercritical Co2 Extraction Of Idesia Polycarpa Oil And Analysis Of Its Fatty Acid Composition, Kuang Chuntao, Wu Bin, Tang Hongwei, Xiang Shu, He Jianyang

Food and Machinery

To make full use of Idesia polycarpa resource, the optimum conditions of extracting oil in I. Polycarpa by supercritical CO2 were studied by single factor and Box- Behnken designs, and the fatty acid composition of this oil was analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the yield of the oil in I. polycarpa extracted by supercritical CO2 was (38.25±0.41)%, when extracted at 24 MPa and 53 ℃ for 147 min. Moreover, the regression model between yield and influence factors was very significant (P=0.000 9). In addition, the results of GC-MS showed that the total content …


Effect Of Different Solvents On Total Phenolic Content, Total Flavonoid Content And Antioxidant Activity Of Highland Barley, Shne Yingbin, Zhang Youwei, Huang Caihuan, Chen Yongsheng, Wang Li, Ou Shiyi, Zhang Hui Nov 2016

Effect Of Different Solvents On Total Phenolic Content, Total Flavonoid Content And Antioxidant Activity Of Highland Barley, Shne Yingbin, Zhang Youwei, Huang Caihuan, Chen Yongsheng, Wang Li, Ou Shiyi, Zhang Hui

Food and Machinery

The objectives of this study were to evaluate total phenolic, total flavonoid content and antioxidant activities of different extraction from three kinds of highland barley. The results showed that 95% ethanol and 60%acetone were better to extract phenolic compounds than other solvents. Total phenolic and total flavonoid contents, antioxidant activities of Zangqing 2000 using different solvents were higher than that of Xunhua and Shangri-la highland barley. Meanwhile, the highest total phenolic content of Zangqing 2000 was 211.92 mg GAE/100 g DW in 60% acetone, the highest total flavonoid content of Zangqing 2000 was 60.11 mg RT/100 g DW in 60% …


Research Advance On Antioxidant Active Composition And Antioxidant Mechanisms Of Edible Fungi, Xiao Xingning, Yuan Ya, Liao Xia, Wang Liying, Shi Fang, Ming Jian Nov 2016

Research Advance On Antioxidant Active Composition And Antioxidant Mechanisms Of Edible Fungi, Xiao Xingning, Yuan Ya, Liao Xia, Wang Liying, Shi Fang, Ming Jian

Food and Machinery

Edible fungi are tasty as well as full of all kinds of biological activity, and the antioxidation is one of the most important activities among them. Polysaccharides, polyphenols, terpenoids,and selenium are the major antioxidants of edible fungi. In this paper, it was reviewed the research advance of antioxidant substances and the antioxidant mechanism of edible fungi, and the future research direction was also summarized, aiming to develop and utilize edible and medicinal values of the polysaccharides in the futher, and improve its application in the fields of food and medicine.


Development And Flavor Substances Analysis Of Purple Sweet Potato And Bitter Buckwheat Combined Vinegar Beverage, Liu Mingyu, Chen Limin, Wang Sidan, Deng Weiqin, Liu Shuliang Nov 2016

Development And Flavor Substances Analysis Of Purple Sweet Potato And Bitter Buckwheat Combined Vinegar Beverage, Liu Mingyu, Chen Limin, Wang Sidan, Deng Weiqin, Liu Shuliang

Food and Machinery

A vinegar beverage combined with purple sweet potato and bitter buckwheat was developed, and the flavor compounds in it were analyzed. Purple sweet potato and buckwheat were processed through smashing, size mixing, saccharification, alcoholic fermentation and acetic fermentation to produce brew purple sweet potato and buckwheat vinegar, respectively.. The optimum formula of the vinegar beverage was obtained through single factor and orthogonal experiments. The 100 mL optimum complex contained 4 mL purple potato vinegar stock solution, 4 mL buckwheat vinegar stock solution,4 g saccharose, and 4 g fructose-glucose syrup. The vinegar beverage prepared under this condition had a unique purple …


Lanthorn, Vol. 51, No. 27, November 28, 2016, Grand Valley State University Nov 2016

Lanthorn, Vol. 51, No. 27, November 28, 2016, Grand Valley State University

Volume 51, July 11, 2016 - June 5, 2017

Lanthorn is Grand Valley State's student newspaper, published from 1968 to the present.


Braden Risk Assessment, Kelly A. Hartley Bsn, Rn, Jessica A. Lobien Bsn, Rn Nov 2016

Braden Risk Assessment, Kelly A. Hartley Bsn, Rn, Jessica A. Lobien Bsn, Rn

Patient Care Services / Nursing

No abstract provided.


Faculty Senate Bill Fsb-2016-11-28-01: University Curriculum Committee, Armstrong State University Nov 2016

Faculty Senate Bill Fsb-2016-11-28-01: University Curriculum Committee, Armstrong State University

Armstrong Faculty Senate: Bills, Motions & Resolutions (Archived)

Faculty Senate Bill FSB-2016-11-28-01: University Curriculum Committee


November 28, 2016 Armstrong Faculty Senate Agenda, Armstrong State University Nov 2016

November 28, 2016 Armstrong Faculty Senate Agenda, Armstrong State University

Armstrong Faculty Senate: Armstrong Faculty Senate Agenda (Archived)

November 28, 2016 Armstrong Faculty Senate Agenda


Examining The Community Food Environments For Elementary School-Aged Children In The City Of London And Middlesex County, Ontario, Catherine Dubreck Nov 2016

Examining The Community Food Environments For Elementary School-Aged Children In The City Of London And Middlesex County, Ontario, Catherine Dubreck

GIS Day

Childhood obesity is a growing public health issue of global importance. In Canada, one in four children and youth are overweight or obese. Research suggests diet plays a crucial role in childhood obesity, and that prevalence rates of overweight or obesity among children in a neighborhood may relate to area socioeconomic characteristics and other environmental factors. The community nutrition environment (e.g., the type, location, and accessibility of food outlets) and the consumer nutrition environment (e.g., the price, promotion, placement, and availability of healthy options and nutrition information) can influence dietary habits, including the specific meal a child orders from a …


Early Evidence Of Stone Tool Use In Bone Working Activities At Qesem Cave, Israel, Andrea Zupancich, Stella Nunziante-Cesaro, Ruth Blasco, Jordi Rosell, Emanuela Cristiani, Flavia Venditti, Cristina Lemorini, Ran Barkai, Avi Gopher Nov 2016

Early Evidence Of Stone Tool Use In Bone Working Activities At Qesem Cave, Israel, Andrea Zupancich, Stella Nunziante-Cesaro, Ruth Blasco, Jordi Rosell, Emanuela Cristiani, Flavia Venditti, Cristina Lemorini, Ran Barkai, Avi Gopher

KIP Articles

For a long while, the controversy surrounding several bone tools coming from pre-Upper Palaeolithic contexts favoured the view of Homo sapiens as the only species of the genus Homo capable of modifying animal bones into specialised tools. However, evidence such as South African Early Stone Age modified bones, European Lower Palaeolithic flaked bone tools, along with Middle and Late Pleistocene bone retouchers, led to a re-evaluation of the conception of Homo sapiens as the exclusive manufacturer of specialised bone tools. The evidence presented herein include use wear and bone residues identified on two flint scrapers as well as a sawing …


The Relationship Between Religiosity And Health-Promoting Behaviors In Pregnant Women, Natalie A. Cyphers, Andrea D. Clements, Glenda Lindseth Nov 2016

The Relationship Between Religiosity And Health-Promoting Behaviors In Pregnant Women, Natalie A. Cyphers, Andrea D. Clements, Glenda Lindseth

ETSU Faculty Works

Pender’s health promotion model guided this descriptive/correlational study exploring the relationship between religiosity and health-promoting behaviors of pregnant women at Pregnancy Resource Centers (PRCs). A consecutive sample included women who knew they were pregnant at least 2 months, could read/write English, and visited PRCs in eastern Pennsylvania. Participants completed self-report surveys that examined religiosity, demographics, pregnancy-related variables, services received at PRCs, and health-promoting behaviors. Women reported they “sometimes” or “often” engaged in health-promoting behaviors, Hispanic women reported fewer health-promoting behaviors than non-Hispanic women, and women who attended classes at the centers reported more frequent health-promoting behaviors than those who did …


Regional Variation In Ascaris Lumbricoides And Trichuris Trichiura Infections By Age Cohort And Sex: Effects Of Market Integration Among The Indigenous Shuar Of Amazonian Ecuador, Theresa E. Gildner, Tara J. Cepon-Robins, Melissa A. Liebert, Samuel S. Urlacher, Felicia C. Madimenos, J. Josh Snodgross, Lawrence S. Sugiyama Nov 2016

Regional Variation In Ascaris Lumbricoides And Trichuris Trichiura Infections By Age Cohort And Sex: Effects Of Market Integration Among The Indigenous Shuar Of Amazonian Ecuador, Theresa E. Gildner, Tara J. Cepon-Robins, Melissa A. Liebert, Samuel S. Urlacher, Felicia C. Madimenos, J. Josh Snodgross, Lawrence S. Sugiyama

Publications and Research

Background: Soil-transmitted helminth (STH) infection peaks during childhood and varies by sex. The impact of market integration (MI) (increasing production for and consumption from a market-based economy) on these infection patterns, however, is unclear. In this study, STH infection is examined by sex and age among indigenous Shuar inhabiting two regions of Amazonian Ecuador: (1) the modestly market-integrated Upano Valley (UV) and (2) the more traditional Cross-Cutucú (CC) region.

Methods: Kato-Katz fecal smears were examined for parasite presence and infection intensity. Factorial ANOVAs and post hoc simple effects analyses were performed by sex to compare infection intensity between regions and …


Anak Dan Jajanan Sekolah: Program Pemberdayaan Kesehatan Anak Sekolah Dalam Perspektif Pemerintah Daerah, Anna Triwijayati, Etsa Astridya Setiyati, Yudi Setianingsih, Maria Lucia Luciana Nov 2016

Anak Dan Jajanan Sekolah: Program Pemberdayaan Kesehatan Anak Sekolah Dalam Perspektif Pemerintah Daerah, Anna Triwijayati, Etsa Astridya Setiyati, Yudi Setianingsih, Maria Lucia Luciana

Media Kesehatan Masyarakat Indonesia

Pemberdayaan anak akan konsumsi makanan jajanan di sekolah merupakan upaya untuk memastikan anak mampu mengambil keputusan konsumsi dengan tepat dan terlindung dari bahaya jajanan yang tidak sehat. Upaya tersebut antara lain menjadi tanggungjawab pemerintah pusat dan daerah. Penelitian ini menggunakan metode kualitatif melalui focus group discussion dengan instansi pemerintah, yaitu Balai Besar Pengawas Obat dan Makanan di Surabaya, Dinas Kesehatan Kota dan Kabupaten Malang serta Dinas Perindustrian dan Perdagangan Kota dan Kabupaten Malang. Hasil diskusi menunjukkan bahwa pemerintah mengambil peran mengatur regulasi yang berkaitan dengan sekolah, kantin dan Pedagang Kaki Lima (PKL) terkait dengan jajanan sehat untuk anak. Namun, di …


Pengaruh Pemberian Ekstrak Daun Kelor Terhadap Kuantitas Dan Kualitas Air Susu Ibu (Asi) Padaibu Menyusui Bayi 0-6 Bulan, Zakaria Zakaria, Veni Hadju, Suryani As'ad, Burhanuddin Bahar Nov 2016

Pengaruh Pemberian Ekstrak Daun Kelor Terhadap Kuantitas Dan Kualitas Air Susu Ibu (Asi) Padaibu Menyusui Bayi 0-6 Bulan, Zakaria Zakaria, Veni Hadju, Suryani As'ad, Burhanuddin Bahar

Media Kesehatan Masyarakat Indonesia

Daun kelor mengandung berbagai macam zat gizi serta sumber fitokemikal. Rendahnya gizi mikro yang dikonsumsi ibu menyusui akan memengaruhi kemampuan untuk menyediakan ASI dengan kandungan gizi mikro yang cukup untuk pertumbuhan bayi. Penelitian ini bertujuan untuk menilai efek dari ekstrak daun kelor terhadap kuantitas dan kualitas ASI pada ibu menyusui. Penelitian ini adalah double blind randomized kontroled design dan dilaksanakan di Kabupaten Maros. Subjek adalah ibu menyusui setelah seminggu melahirkan dan dibagi kedalam dua kelompok. Kelompok pertama menerima ekstrak daun kelor (EK) dua kali dua kapsul, 800mg/kapsul, (kelompok EK, n=35) dan kelompok lainnya menerima tepung daun kelor (TE) dengan dosis …


Faktor Risiko Stunting Pada Anak Usia 6-24 Bulan Di Kota Bogor, Erni Rukmana, Dodik Briawan, Ikeu Ekayanti Nov 2016

Faktor Risiko Stunting Pada Anak Usia 6-24 Bulan Di Kota Bogor, Erni Rukmana, Dodik Briawan, Ikeu Ekayanti

Media Kesehatan Masyarakat Indonesia

Pertumbuhan anak merupakan salah satu indikator status gizi dan kesehatan dalam populasi untuk mencerminkan kualitas sumber daya manusia. Stunting yaitu pertumbuhan linier yang terhambat dan merupakan hasil dari ketidakcukupan gizi dalam jangka waktu lama. Penelitian ini bertujuan untuk menganalisis faktor-faktor risiko stunting pada anak usia 6-24 bulan di Kota Bogor. Penelitian ini merupakan desain crossectional dengan subjek 360 anak usia 6-24 bulan di Kota Bogor. Teknik pengumpulan data menggunakan kuesioner yang terdiri dari keadaan sosial ekonomi orang tua, yaitu pendidikan, pekerjaan, dan pendapatan. Berat badan dan panjang badan lahir berdasarkan data yang diambil dari KMS dan buku KIA. Data yang …


The Relationship Between Chewing Food And Sleeping Disorders Caused By Dental And Oral Diseases To Preschool Children’S Food Intake, Ristya Widi Endah Yani Nov 2016

The Relationship Between Chewing Food And Sleeping Disorders Caused By Dental And Oral Diseases To Preschool Children’S Food Intake, Ristya Widi Endah Yani

Media Kesehatan Masyarakat Indonesia

Food intake is a directly influential factor in determining nutritional status of preschool children. Dental and oral diseases generally cause pain in teeth which make them have difficulty to eat and sleep well. This situation can result in reduced food intake. The research objective was to analyze the relationship between chewing food and sleeping disorders caused by dental and oral diseases to preschool children’s food intake. An analytic observational study with cross sectional design in preschool age children was conducted in District Ambulu, Jember Regency. A hundred and two samples were taken using purposive sampling method. The variables studied were …


Volume 54, Number 15: November 23, 2016, University Of North Dakota Nov 2016

Volume 54, Number 15: November 23, 2016, University Of North Dakota

University Letter Archive

No abstract provided.


Validity Of Methods In Measuring Resting Energy Expenditure In Pediatric Oncology Patients, Ashley Marie Hobar Nov 2016

Validity Of Methods In Measuring Resting Energy Expenditure In Pediatric Oncology Patients, Ashley Marie Hobar

Electronic Theses and Dissertations Archive

Evaluation of nutrition status is imperative for pediatric oncology patients due to prevalence of malnutrition and its effects. Methods used to evaluate nutrition status over- and underestimate energy needs. The purpose of the project was to determine if the Medgem and WHO predictive equations predict resting energy expenditure (REE) when compared to a traditional indirect calorimeter. Participants, 7-18 years of age, had testing completed on a traditional indirect calorimeter, Medgem, and energy estimation by WHO equation. Post hoc pairwise comparisons revealed all three methods were significantly different from one another (all p<0.0001), suggesting REE be measured by metabolic cart.


The Influence Of Plant- And Animal-Based Diets On Testosterone And Lean Body Mass Is Male Rats, Damien Christopher Moore Nov 2016

The Influence Of Plant- And Animal-Based Diets On Testosterone And Lean Body Mass Is Male Rats, Damien Christopher Moore

Electronic Theses and Dissertations Archive

Background: It is commonly believed but unproven that plant-based diets result in low circulating testosterone, with minimal gains in lean body mass (LBM). Methods: Long-Evans rats (N=28) were assigned to experimental diets with either plant (PD) or animal (AD) protein sources. Animals were further divided into two additional conditions without and with exercise. Animals were fed ad libitum for 3 months and at the end of month three blood was collected for measurement of testosterone and estradiol concentrations. Dual x-ray absorptiometry assessed body composition. Results: Neither blood testosterone concentrations nor LBM differed between rats fed the PD or AD. Conclusion: …


An Assessment Of College Tuition Increases And Retention, Raymond N. Karasek Iii Nov 2016

An Assessment Of College Tuition Increases And Retention, Raymond N. Karasek Iii

Electronic Theses and Dissertations Archive

This study examines the relationship between tuition increases, retention, and Pell Grant recipients attending college for the first time at 279 four-year public universities in the southeastern and southwestern regions of the United States. The data for this study was gathered from the Integrated Postsecondary Education System (IPEDS) using the tuition, retention, and Pell Grant information collected in the fall of those corresponding academic years. APearson moment correlation and linear regression analysis was used to find significance between the three variables. The study revealed that there was no significant interaction with student retention. However, the study did reveal a correlation …


Analisis Determinan Karakteristik Keluarga Dan Pemenuhan Hak Kesehatan Anak Serta Dampaknya Terhadap Status Gizi, Emillia Rahmariza, Ikeu Tanziha, Dadang Sukandar Nov 2016

Analisis Determinan Karakteristik Keluarga Dan Pemenuhan Hak Kesehatan Anak Serta Dampaknya Terhadap Status Gizi, Emillia Rahmariza, Ikeu Tanziha, Dadang Sukandar

Media Kesehatan Masyarakat Indonesia

Kota Depok pada tahun 2013 menerima penghargaan Kota Layak Anak (KLA) dalam peringatan Hari Anak Nasional. Depok sebagai Kota Layak Anak diharapkan dapat memenuhi hak kesehatan anak. Disisi lain, jumlah balita gizi kurang dan gizi buruk di Kota Depok adalah 5138 orang atau sebanyak 4.62%. Nilai ini meningkat dari tahun 2011 yaitu sebesar 4.4%. Penelitian ini bertujuan menganalisis pemenuhan hak kesehatan dan dampaknya terhadap status gizi anak. Desain penelitian menggunakan cross sectional study. Penelitian dilaksanakan dari bulan Juni-Agustus 2013 di Kelurahan Cipayung Jaya dan Kelurahan Bojong Pondok Terong, Depok. Subjek dalam penelitian ini adalah anak yang berusia 0-18 tahun. Penelitian …