Digital Commons Network^™

Intracellular Ros Mediates Gas Plasma-Facilitated Cellular Transfection In 2d And 3d Cultures, Dehui Xu, Biqing Wang, Yujing Xu, Zeyu Chen, Qinjie Cui, Yanjie Yang, Hailan Chen, Michael G. Kong

Bioelectrics Publications

This study reports the potential of cold atmospheric plasma (CAP) as a versatile tool for delivering oligonucleotides into mammalian cells. Compared to lipofection and electroporation methods, plasma transfection showed a better uptake efficiency and less cell death in the transfection of oligonucleotides. We demonstrated that the level of extracellular aqueous reactive oxygen species (ROS) produced by gas plasma is correlated with the uptake efficiency and that this is achieved through an increase of intracellular ROS levels and the resulting increase in cell membrane permeability. This finding was supported by the use of ROS scavengers, which reduced CAP-based uptake efficiency. In …

Ethical Leadership In Social Enterprises : Multilevel Investigation Of Its Influence On Team And Individual Prosocial Voice, Pok Man Tang

Lingnan Theses

This research paper seeks to draw on social learning theory (Bandura, 1977) as an overarching framework to examine how unit managers’ ethical leadership style affects the team and individual prosocial voice behaviors in the context of social enterprises in Hong Kong.

Ethical leadership has been found to be conducive to both desirable team and individual employee behaviors. However, scholarly understanding of the multi-level effects of ethical leadership and the underlying mechanisms involved is rather limited. Moreover, previous research has directed attention almost exclusively to the influence of ethical leadership in the context of commercial organizations. This narrow stance has curiously …

Genetic And Cellular Studies Highlight That A Disintegrin And Metalloproteinase 19 Is A Protective Biomarker In Human Prostate Cancer, Gerard F. Hoyne, Caoline Rudnicka, Qing-Xiang Sang, Mark Roycik, Sarah Howarth, Peter Leedman, Markus Schlaich, Patrick Candy, Vance Matthews

Health Sciences Papers and Journal Articles

Background: Prostate cancer is the second most frequently diagnosed cancer in men worldwide. Current treatments include surgery, androgen ablation and radiation. Introduction of more targeted therapies in prostate cancer, based on a detailed knowledge of the signalling pathways, aims to reduce side effects, leading to better clinical outcomes for the patient. ADAM19 (A Disintegrin And Metalloproteinase 19) is a transmembrane and soluble protein which can regulate cell phenotype through cell adhesion and proteolysis. ADAM19 has been positively associated with numerous diseases, but has not been shown to be a tumor suppressor in the pathogenesis of any human cancers. Our group …

How Does Exposure To The Internet Affect Political Knowledge And Attitudes Among Rural Chinese?: A Field Experiment, Wenwen Shi

Wayne State University Dissertations

The Internet's political implications reach far beyond an advanced communication device in authoritarian regimes. The heated debate on the Internet's political potential in China is complicated by a lack of causal evidence demonstrated in the literature. Thus far, studies have only examined consequences of the rise of the Internet and the characteristics of netizens (wangmin), but they have failed to account for the possible transformative effect the Internet has on individual citizens. This study attempts to fill this gap by testing the impact of the Internet on rural Chinese villagers' political knowledge and political attitudes through a field experiment over …

Emerging Genres Of Online Technical Communciation, Luke Anthony Thominet

Wayne State University Dissertations

Emerging Genres of Online Technical Communication is a study of how the proliferation of non-professional participation has the potential to significantly change the shape of technical communication. More specifically, I use a genre analysis methodology to investigate three forms of user-generated content: crowdsourced documentation wikis, video games user reviews, and video game open development. In the first study, I analyze five crowdsourced documentation wikis and find systemic inconsistency in the workflow and content quality of the documentation. Subsequently, I argue that practitioners should use minimalist documentation theory to design more effective user-centered author support for the wikis. My second chapter …

Saving Innocents: Tracing The Human Monster Hunter’S Hetero-Normative Agenda From The 1970s To Today, Adam Kem Yerima

Wayne State University Dissertations

In supernatural-themed American television programs that focus on hunting or exposing monsters and the monstrous, the human monster hunter’s representation and actions promote a conservative subtext. While horror scholarship prefers to examine the genre’s monsters and women, and regulate the hunter as simply being an expert, an exploration into the historical development of the figure reveals that there is a lot more to this character. The human monster hunter’s televisual depictions from the 1970s to today reveal a complex illustration that reifies the “American dream.” Time and time again, through the loss of an ideal middle-class experience, the human monster …

Novel Regulatory Mechanisms Of Inositol Biosynthesis In Saccharomyces Cerevisiae And Mammalian Cells, And Implications For The Mechanism Underlying Vpa-Induced Glucose 6-Phosphate Depletion, Wenxi Yu

Wayne State University Dissertations

Myo-inositol is the precursor of all inositol containing molecules, including inositol phosphates, phosphoinositides and glycosylphosphatidylinositols, which are signaling molecules involved in many critical cellular functions. Perturbation of inositol metabolism has been linked to neurological disorders. Although several widely-used anticonvulsants and mood-stabilizing drugs have been shown to exert inositol depletion effects, the mechanisms of action of the drugs and the role of inositol in these diseases are not understood. Elucidation of the molecular control of inositol synthesis will shed light on the pathologies of inositol related illnesses.

In Saccharomyces cerevisiae, deletion of the four glycogen synthase kinase-3 genes, MCK1, MRK1, MDS1, …

Social Media Adoption Among University Instructors In Saudi Arabia, Khalid Abdulaziz Alasfor

Wayne State University Dissertations

There is an orientation from the government of Saudi Arabia toward social media in general. The government of Saudi Arabia considers the significance of social media in educating the Saudi community. The Saudi Ministry of Education founded the National Center for E- Learning and Distance Learning (NCeL) because it considered the importance of e-learning and distance learning for higher education (he.moe.gov.sa). NCeL supports and rewards university instructors to integrate social media in the learning process (award.elc.edu.sa). Several studies proved the significance of social media with Saudi higher education students (Alkhalifa, 2008; Salem and Alghamdi, 2011; Almadhouni, 2011). Moreover, Saudi students …

Stop Identity Cues As A Cue To Language Identity, Paula Lisa Castonguay

Wayne State University Dissertations

The purpose of the present study was to determine whether language membership could potentially be cued by the acoustic-phonetic detail of word-initial stops and retained all the way through the process of lexical access to aid in language identification. Of particular interest were language-specific differences in CE and CF word-initial stops. Experiment 1 consisted of an interlingual homophone production task. The purpose of this study was to examine how word-initial stop consonants differ in terms of acoustic properties in Canadian English (CE) and Canadian French (CF) interlingual homophones. The analyses from the bilingual speakers in Experiment 1 indicate that bilinguals …

Novelistic Intimacies: Reading And Writing In The Late Age Of Print, Vincent Michael Haddad

Wayne State University Dissertations

In Novelistic Intimacies, I consider the political and aesthetic structure of intimacy in a diverse set of narrative forms produced in the so-called digital age, or the late age of print—from encyclopedic and metafictional novels to graphic storytelling and Afrofuturist fantasy. As an organizing principle, intimacy forces us to consider, at once, how novelists have attempted to restore language and narrative with personal meaning after postmodernism—often termed New Sincerity or post-irony. At the same time, intimacy allows us to see how novelists have experimented on the materiality of the book and the eroticism of language to invent new, impersonal modes …

Memes, Args And Viral Videos: Spreadable Media, Participatory Culture, And Composition Pedagogy, Mary Karcher

Wayne State University Dissertations

This project argues that spreadable media texts motivate people to engage in compositional activities advocated in First Year Composition (FYC). Drawing on Henry Jenkins’ assertion that participatory culture offers potential for learning, I use his list of eleven participatory culture skills that he believed necessary for all students. After showing how well the Participatory Culture Abilities (PCAs) align with the WPA Outcomes Statement (WPA OS), I put forth the WPA OS and the PCAs combined as a lens through which to view three spreadable media case studies: Spreadable Media Events, Fan Labor, and Alternate Reality Games. Based on my findings, …

The Affective Presidency, John Patrick Koch

Wayne State University Dissertations

This dissertation explores the relationship between affect, political emotions, and presidential rhetoric. In examining the political philosophy and presidency of Woodrow Wilson, this dissertation explores how presidential rhetoric captures, channels, and/or directs the passions of the people. Drawing on research by Sarah Ahmed and Brian Masummi, this dissertation argues that presidential rhetoric intervenes into the affective process by directing the passions of citizens towards promises of happiness and investment in presidential power. Two case studies, one focusing on Wilson’s tour in support of the League of Nations and the other on presidential museums, highlight the affective function of presidential rhetoric. …

Using R2d2 To Create Information Literacy Objects In Academic Libraries: Design-Based Research, Kristin Orlich Lavoie

Wayne State University Dissertations

Academic librarians at the university level are increasingly called upon to create information literacy objects which are available to students online. These librarians, however, frequently have little or no training in any type of instruction, either face to face or online. Because of the unique attributes of online learning, librarians should be aware of instructional design models and learner preferences in order to maximize online student learning. Academic librarians’ utilization of the activities which promote efficacious online learning can be increased through awareness of an instructional design model best suited to this purpose. Research was needed to develop a guide …

Calponin And Cytoskeleton Dynamics In Macrophage Functions And The Pathogenesis Of Atherosclerosis, Rong Liu

Wayne State University Dissertations

Arterial atherosclerosis is an inflammatory disease. Macrophages play a major role in the pathogenesis and progression of atherosclerotic lesions. Modulation of macrophage function is a therapeutic target for the treatment of atherosclerosis. Calponin is an actin-filament-associated regulatory protein that inhibits the activity of myosin-ATPase and dynamics of the actin cytoskeleton. Encoded by the Cnn2 gene, calponin isoform 2 is expressed at significant levels in macrophages. Deletion of calponin 2 increases macrophage migration and phagocytosis. In the present study, we investigated the effect of deletion of calponin 2 in macrophages on the pathogenesis and development of atherosclerosis. The results showed that …

Cardiolipin Is Required For Optimal Acetyl-Coa Metabolism, Vaishnavi Raja

Wayne State University Dissertations

The phospholipid cardiolipin (CL) is crucial for many cellular functions and signaling pathways, both inside and outside of mitochondria. My thesis focuses on the role of CL in energy metabolism. Many reactions of electron transport and oxidative phosphorylation, the transport of metabolites needed for these processes, and the stabilization of electron transport chain supercomplexes, require CL. Recent studies indicate that CL is required for the synthesis of iron-sulfur (Fe-S) co-factors, which are essential for numerous metabolic pathways. Activation of carnitine-acetylcarnitine translocase, which transports acetyl-CoA into the mitochondria, is CL dependent. The presence of substantial amounts of CL in the peroxisomal …

Navigating The Transition Into Motherhood: Women's Experiences Of Control, Emotions, And Social Ideals, Jody Sue Sauer-Sargent

Wayne State University Dissertations

In this dissertation, I sought to give postpartum women their own voices so that they could help define the postpartum experience on their own terms. It fills important gaps within the literature on new mothers’ experiences. A phenomenological approach was used, emphasizing the lived experiences of the women, with an overlay of autoethnography, where the personal experience of the researcher becomes important primarily in how it illuminates the phenomenon being studied. Thus, my personal experience of pregnancy into early motherhood is interwoven throughout this dissertation. Forty-two women participated in the in-depth, face-to-face interview, followed by a questionnaire. The qualitative data …

Loss Of Rap1gap: A Driver In The Progression From Dcis Of The Breast To Idc, Seema Shah

Wayne State University Dissertations

The purpose of this study is to determine the role of Rap1Gap in the progression of DCIS (ductal carcinoma in situ) to IDC (invasive ductal cancer). We employed an in-vitro three-dimensional (3D) overlay model that provides a physiologically relevant microenvironment to study mechanisms of malignant progression. Previous studies from this laboratory aimed to determine specific gene expression changes common to three models of DCIS- MCF10.DCIS, SUM 102 and SUM 225 in comparison to MCF10A cells, a model of non-transformed human mammary epithelium. The expression of 295 genes was found to be significantly altered, with 63 being increased in expression in …

Structural Characterization And Therapeutic Utility Of The Proton-Coupled Folate Transporter, Michael Roy Wilson

Wayne State University Dissertations

Folate is a B9 vitamin essential to DNA synthesis. The proton-coupled folate transporter (PCFT) is a newly discovered proton/folate symporter with an acidic pH optimum and broad expression across a variety of solid tumor types, with limited expression in normal tissues. Several antifolate molecules have been developed as cancer therapeutics, although these classical antifolates display numerous off-target effects due to transport by the ubiquitous reduced folate carrier (RFC). In this dissertation, we determine the roles of multiple PCFT structure/function domains, and develop PCFT-specific antifolates to target solid tumors. We utilize substituted cysteine accessibility methods (SCAM) to identify a novel reentrant …

Student, Teacher, School, And District Determinants Of Elementary School Accountability Classification In The Detroit Metropolitan Region, Embekka Nakia Thompson

Wayne State University Dissertations

Legislators have implemented an accountability system in an effort to increase school quality for all students. Policymakers assume that rewards and sanctions, based on results from high-stakes testing, can improve student achievement. The accountability system does not consider environmental factors that impact student outcomes. This study examines student, teacher, school and district determinants and their impact on student achievement, school designation, and school ranking. Data was collected from 333 elementary (K-5) schools in 56 public school districts and 18 charter schools in three counties: Macomb, Oakland, and Wayne. Two research questions were developed for this study.The first research question examined …

The Journey, Feiyi Bie, Fleet Library, Special Collections

3rd Student Artists' Book Contest 2017

No abstract provided.

V.1 2016-2017, Center For Arts & Language, Students Of Risd, Risd Archives

All Student Newspapers

v.1 is a publishing platform for the ongoing work, ideas, discussions, debates, and aspirations of students at the Rhode Island School of Design. The 2016-2017 issue contains articles on Kara Walker, Women's March, "Look at Art, Get Paid" program, #pizzagate, the Mutual Encounters exhibition, and smartphones.

Toward An Engineering Discipline Of Warehouse Design, Leon Mcginnis, Timothy Sprock

14th IMHRC Proceedings (Karlsruhe, Germany – 2016)

Warehouses today are complex dynamic engineered systems, incorporating automation, mechanization, equipment, fixtures, computers, networks, products and people, and they can support the flow of tens or hundreds of thousands of different items to enable fulfilling thousands or tens of thousands of orders daily. In that sense, they represent a design challenge that is not terribly different from the design of other complex dynamic engineered systems, such as a modern passenger airplane, an automobile, or a unique building. What is different is that the design of these other complex dynamic engineered systems typically follows some engineering design discipline. Here, we argue …

Seccll Program [2016]

South East Coastal Conference on Languages & Literatures (SECCLL)

N/A

Mormon Studies Review Volume 3, Mormon Studies Review

Mormon Studies Review

No abstract provided.

Checklist Of The Dolichopodidae (Diptera, Brachycera) Of Turkey, Alper Tonguç, Igor Grichanov, Stefan Naglis

Turkish Journal of Zoology

The first checklist of the Dolichopodidae of Turkey is presented. A total of 187 species and one subspecies belonging to 35 genera are listed. The type locality and the distribution in Turkey for each species are given.

In Re Yahoo Mail Litigation, Northern District Of California

Historical and Topical Legal Documents

NOTICE OF MOTION AND MOTION FOR PRELIMINARY APPROVAL OF CLASS ACTION SETTLEMENT AND MEMORANDUM OF POINTS AND AUTHORITIES IN SUPPORT

Grupo Cultural Afroreggae V. Google, Inc., California Superior Court

Historical and Topical Legal Documents

No abstract provided.

Application Of A Time Delay Neural Network For Predicting Positive And Negative Links In Social Networks, Saghar Babakhanbak, Kaveh Kavousi, Fardad Farokhi

Turkish Journal of Electrical Engineering and Computer Sciences

No abstract provided.

Analyzing The Mutual Authenticated Session Key In Ip Multimedia Server-Client Systems For 4g Networks, Bakkiam David Deebak, Rajappa Muthaiah, Karuppuswamy Thenmozhi, Pitchai Iyer Swaminathan

Turkish Journal of Electrical Engineering and Computer Sciences

No abstract provided.

Does Large Family Size Predict Political Centrism?, Benjamin Schmidt

Sigma: Journal of Political and International Studies

Suggesting that voting might be correlated with the number of children voters have has been rare but not unheard of in the last decade. In a 2004 article for American Conservative, Steve Sailer noted a correlation between states with higher birth rates among white voters and the support for incumbent Republican President George W. Bush. Sailer recognized that Bush won the nineteen states with the highest white fertility while Senator John Kerry won the sixteen with the lowest (2004). He also suggested that the lifestyle preferences of white, conservative parents might be to blame for the apparent Republican tilt among …