Digital Commons Network^™

Efficiency Of The Nuclease I-Scei In Excising Selectable Marker Genes From The Plant Genome, Elliot E. Pruett, Soumen Nandy, Vibha Srivastava

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Gene stacking is a method used in biotechnology by which multiple genes can be placed at a single genomic site, thereby simplifying plant breeding. In this approach, DNA nucleases are used for excising selectable marker genes (SMG), which are the unneeded components of transgenic plants. The goal of this project is to evaluate the effectiveness of the nuclease I-SceI in excising DNA in plants. Specifically, this study tests heat-inducible I-SceI through the use of a heat-shock promoter (HS) in order to control SMG excision by heat application. The DNA plasmid containing a visual marker gene flanked by I-SceI target sites …

Wright Is Still Wrong: The Wright Amendment Reform Act And Airline Competition At Dallas Love Field, Barrett V. Armbruster

Journal of Air Law and Commerce

No abstract provided.

Fingerprints: A New Means Of Identification In Airport Security Screening, Chase Hilton

Journal of Air Law and Commerce

No abstract provided.

Safety In The Sky: Will Reforming And Restructuring The Tsa Improve Our Security Or Merely Infringe On Our Rights?, Katherine A. Lowe

Journal of Air Law and Commerce

No abstract provided.

Airline Security And Employee Immunity: The Second Circuit Promotes Airline Security Interests At All Costs—Even If It Means Throwing Efficiency And Accountability By The Wayside, Landon M. Hankins

Journal of Air Law and Commerce

No abstract provided.

Hijacking Open Skies: The Line Between Tough Competition And Unfair Advantage In The International Aviation Market, Hannah E. Cline

Journal of Air Law and Commerce

No abstract provided.

Comparison Of The Effects Of Energy Flux On Metabolic Conditions And Satiety In Young Adults, Molly Madison Demello

Theses and Dissertations

Within the United States, 68% of all adults have been classified as overweight to obese, as defined by 25 kg/m2 ≤ BMI ≤ 35 kg/m2. Research has shown that overweight/obese individuals can still gain health benefits through participation in aerobic activity without losing weight. These improvements include improved glucose control, CRF, HDL and quality of life. More research is necessary to understand the health benefits associated with a chronic aerobic intervention under weight stable conditions. We therefore examined the health benefits of an aerobic 6-month intervention under weight stable conditions on body composition, cholesterol and measurements of appetite and satiety …

Factors Influencing The Collagen Fiber Angle Distribution In The Mouse Aorta, Shana Roach Watson

Theses and Dissertations

The aortic extracellular matrix (ECM) consists of microstructural proteins, collagen and elastin, together with proteoglycans and other components. The matrix metalloproteinases (MMPs) are proteolytic enzymes that influence morphological and structural changes in the ECM and can degrade the matrix as it responds to cellular behaviors such as angiogenesis, apoptosis, proliferation, and migration. Collagen is the most important component among the extracellular proteins because it provides strength and stability to the tissue. Changes in collagen content play a major role in the development of atherosclerosis. These changes can be induced by increased or decreased proteinase activity. Therefore, we studied the collagen …

Rallying Education Activism From The Grassroots Up: A Case Study Of The South Carolina Education Improvement Act Of 1984, Sally Saunders Huguley

Theses and Dissertations

With the 1983 publication of A Nation At Risk that warned of the “rising tide of mediocrity” in American public education, southern governors took the lead in proposing reforms to their states’ lagging education systems. Among those was South Carolina Governor Richard W. Riley, who proposed public school reform legislation funded by a statewide one cent sales tax increase within the difficult context of a legislative election year. Facing stiff opposition to the tax increase from the South Carolina General Assembly, Riley launched a statewide grassroots effort to pressure the Legislature to enact the 1984 Education Improvement Act (EIA) and …

A Phenomenological Analysis Of Social Justice/Injustice On The Lives Of African-American High School Males, Michael R. Harris

Theses and Dissertations

The purpose of this study is to analyze what high school African-American males perceive as significant influences that directly impact their academic achievement while attending a predominantly White suburban high school. This qualitative research study will utilize semi-structured case interviews to collect and analyze data gathered from among a criteria-based subgroup of African-American high school males. An examination of archival data such as student course selections, a review of high school Carnegie units earned and attempted, the availability of support resources and interventions, as well discipline and attendance reports will each provide evidence of the probable obstacles impacting the achievement …

A Theoretical Study Of Polymer Based Drug Delivery Systems, Ebtisam Abdullatif Aldaais

Theses and Dissertations

Polymer nanostructured materials for drug delivery applications have witnessed tremendous progress in recent years due to their vast potential. One-end-grafted polymers can form grafted micelles with specific mechanical properties. Biological conditions can alter these properties resulting in the protection or release of drugs. Charged surfactants can also form micelles in an aqueous solution, which can also be manipulated through special conditions. On the micellar surface, self-organized polyelectrolytes can be stimulated to extend their attached ligands and thus increase the probability of binding to targeted receptors. This thesis focuses on modeling polymer-based drug delivery systems by studying the physical interactions between …

Promoting Progress To Assist Youth With Disordered Eating In School Mental Health, Bryn Elizabeth Schiele

Theses and Dissertations

Disordered eating has become a significant issue among children and adolescents; nearly 14% of all youth displaying disordered eating patterns. Despite the prevalence of these disorders amongst school-age students, there is a deficit of empirical literature on the integration of eating disorder support services in schools, as well as a lack of knowledge and training of school mental health (SMH) professionals regarding the appropriate interventions for this population. While eating disorders have previously been considered as outside of the school mental health domain of practice (e.g., Judge, 2001), this view has changed and there exists a significant need to provide …

Progesterone-Only Oral Contraceptive Pill, Breast Cancer, Heart Disease, And Stroke, Marsha Ema Samson

Theses and Dissertations

There is evidence from earlier studies that oral contraceptive pills may be a risk factor for certain chronic diseases, including heart disease, stroke, and breast cancer. Previous studies mainly focus on the estrogen component of combined (estrogen+ progestin) oral contraceptives (COCs) due to their popularity. This focus limits our understanding of progestin-only contraceptives and its relationship to commonly occurring chronic diseases. To provide insight into alternative methods of oral contraception, this dissertation explores the relationship between progestin-only oral contraceptive (POC) pills and heart disease, stroke, and breast cancer. We hypothesize that women using POCs are less likely to have certain …

Uptake Of Nanoparticles By Vibrio Gazogenes, Shonda Renee Jones

Theses and Dissertations

Vibrio gazogenes, a Gram-negative species of marine bacteria, was capable of transporting 20 nm (diameter) surface-carboxylated, polystyrene, fluorescent FluoSpheres® microspheres (excitation/emission = 505/515) through the outer membrane, which was indicated by a detectable decrease in the fluorescence intensity of the nanoparticles in the culture medium. A mechanism of transport was investigated involving the ATP-binding cassette (ABC) protein transporters that traverse the outer membrane. Inhibition of the ABC transporters did not prevent the entry of the nanoparticles into the cell, suggesting there was an alternate mechanism of transport. The addition of nanoparticles to the culture medium also did not provide any …

Adaptive Skill Trajectories In Infants With Fragile X Syndrome, Kelly Elizabeth Caravella

Theses and Dissertations

This study examined longitudinal trajectories of adaptive behavior in infants with fragile X syndrome (FXS), compared to typical development (TD) and infant siblings of children diagnosed with autism (ASIBs). Additional analysis were conducted to examine the differences in trajectories for males and females with FXS, and to identify if a profile of strengths and weaknesses appeared across domains by 24 months in males with FXS. Participants included 76 male infants assessed up to 4 times between 6 and 24 months of age. A sample of 12 females with FXS was also included for the comparative sex analysis. Infants with FXS …

Structural, Functional, And Behavioral Alterations To The Dopamine System In The Female Hiv-1 Transgenic Rat, Robert Francis Roscoe Jr.

Theses and Dissertations

HIV-associated cognitive disorders continue to affect approximately 25 million individuals worldwide, and its prevalence is expected to increase as the lifespan of HIV-1 infected individuals continues to improve. The HIV-1 transgenic rat expresses 7 of the 9 genes that encompass the virus, and is an appropriate model for studying chronic HIV-1 infection at a level that is controlled through combined antiretroviral therapy. Psychostimulant abuse is known to exacerbate HAND symptomology, but the psychostimulant methylphenidate is a first line of treatment for HIV associated cognitive inhibition. Dopamine transport abnormalities in the HIV-1 Tg rat have been well characterized through behavioral testing; …

Development Of Protein-Polymer Core-Shell Nanoparticles (Ppcs-Nps) As Efficient Vehicles To Deliver Therapeutic Agents Across Blood Brain Barrier (Bbb), Napat Tandikul

Theses and Dissertations

Blood Brain Barrier (BBB) plays a main role as selective barrier which controls and limits access of chemicals, molecules and therapeutic agents from blood to brain. The BBB endothelial cells are connected by Tight Junctions (TJs) which close intracellular spaces between the endothelial cells and block the free diffusion of substances, therefore many potential drugs for treating human brain diseases cannot reach the brain in sufficient concentration. Recently, many studies have thrown an interest in development of nanoparticles for delivering drugs and imaging agents across BBB. Our research group has developed protein-polymer core-shell nanoparticles (PPCS-NPs) which demonstrate great potential for …

Memory Of A Landscape, Elizabeth Halliday

Theses and Dissertations

Artist Statement

Creativity is one of my strongest talents. As a visual person, I understand and interpret the world through observation and analysis. Nature intrigues me. I find it awe inspiring how the natural environment can change so dramatically day-to-day. As a result, I record my experiences and memories of landscapes and seascapes.

My areas of interest are painting and computers in the arts. I am an abstract painter and my media consists of acrylic, watercolor, mixed media, and digital imagery. I create low relief and rough textures with added materials, and develop layers of paint with a palette knife. …

Chaotic Journey, Cheryl Ann Seif-Regan Mrs.

Theses and Dissertations

Artist Statement

My art is about seeking answers to personal conflicts while telling a story of a

chaotic journey. I reflect on everyday moments and my thoughts as I discover ways to

make sense of situations and life. I do this by creating textural, vibrantly colored, and

gestural surfaces that emulate the powerful waters of the seas. I want to reveal an

emotionally driven and process-oriented experience to the viewer. While creating, I do

not maintain full control of the media and let the process become part of the work.

I aggressively layer thick paint, glass, and mixed media. I …

Strata, Lauren Hale

Theses and Dissertations

In my mixed media pieces and sculptural books, I explore the significance of place. Place is not just a geographical location; it is a layered history that tells a story. My experiences in a specific environment and how I relate to it, study it, remember it, or imagine it drive me to create pieces. While the choices I make are influenced by my own experiences, I want to evoke feelings of familiarity, intrigue, and a sense of nostalgia.

In my process, I intuitively layer paint, printed materials, and mixed media to make a foundation for exploration. I continue to …

Information Needs And Information Sources Of Patients Diagnosed With Rare Cancers, Dana L. Ladd

Theses and Dissertations

Abstract

INFORMATION NEEDS AND INFORMATION SOURCES OF PATIENTS DIAGNOSED WITH RARE CANCERS

By Dana L. Ladd, Ph.D., MS, SLIS

BACKGROUND: Approximately 25% of all cancers diagnosed are considered rare. Patients may face many significant challenges including difficulty obtaining information about their rare conditions. Patients often have high information needs and may seek desired information from a variety of informational sources including healthcare providers, media, print, government and non-profit organizations in order to meet their needs. Accessing reliable consumer-level information can be challenging and often information needs are unmet. Dissatisfaction with health information provision can result in negative health-related outcomes and …

Genetic And Epigenetic Mechanisms Of Complex Reproductive Disorders, Bhavi P. Modi

Theses and Dissertations

Common, complex disorders are polygenic and multifactorial traits representing interactions between environmental, genetic and epigenetic risk factors. More often than not, contributions of these risk factors have been studied individually and this is especially true for complex reproductive traits where application of genomic technologies has been challenging and slow to progress. This thesis explores the potential of genetic and epigenetic components contributing to a better understanding of the biological pathways underlying disease risk in two specific female complex reproductive traits - polycystic ovary syndrome (PCOS) and preterm premature rupture of membranes (PPROM). The PCOS projects focus on characterization of a …

Non-Medical Use Of Prescription Stimulants For Weight Loss: Predictors, Consequences, And Implications For Intervention In A National Young Adult Sample, Amy J. Jeffers

Theses and Dissertations

The non-medical use of prescription drugs is an important public health concern. Non-medical use of prescription stimulants (NMUPS), specifically medications used to treat Attention Deficit Hyperactivity Disorder (ADHD), is particularly concerning. One timely concern regarding NMUPS, especially among young adults, is their role in appetite suppression/weight loss. Indeed, some individuals are motivated to misuse such drugs for the purpose of losing weight. Engaging in NMUPS for weight loss has been examined only minimally in the research literature. However, extant data demonstrate that this behavior is associated with other unhealthy behaviors and poor psychosocial health. Limitations of prior research include the …

Curcumin/Melatonin Hybrids As Neuroprotective Agents For Alzheimer's Disease, John Saathoff

Theses and Dissertations

Alzheimer's disease (AD) is a progressive neurodegenerative disorder and the leading cause of dementia, affecting ~5.2 million Americans. Current FDA approved medications provide mainly symptomatic relief and there are no agents available to delay or cure this disease. Multiple factors such as amyloid-β aggregates, dyshomeostasis of biometals, oxidative stress, and neuroinflammation have been implicated in the development of AD. Even though significant advances have been made in understanding the mechanisms leading to AD, the exact etiology still remains elusive. Given AD’s multifactorial nature, a multifunctional strategy of small molecule design would help to identify novel chemical templates. Recently our lab …

The Instructional Technology Resource Teacher: A Descriptive Case Study Of Deployment, Use, And Perceptions, Mary Sepelyak

Theses and Dissertations

This case study describes one professional development approach to support technology integration at all public schools in one large county in central Virginia. Using data obtained from daily time logs, the frequency of Instructional Technology Resource Teacher (ITRT) use by classroom teachers was analyzed. Descriptive statistics were used to describe overall percentage of ITRT use, the various types of professional development requested by teachers, the consistency of those activities over time, and if the frequencies of activities varied as a function of school level, Title I status at the elementary level, or subject area taught by teachers at the secondary …

The New Gatekeepers: How Blogs Subverted Mainstream Book Reviews, Rebecca E. Johnson

Theses and Dissertations

Book reviewing has a fraught history in the United States. Reviewers have long been accused of not being analytical enough. It should be no wonder then with the emergence of social media that online book reviewing has become increasingly popular. Online reviewers, especially book bloggers, are no literary gatekeepers in their own right, shaping the tastes of readers across the world. Book blogs in particular pay special attention to titles which have long been derided by institutions such as libraries, academia, publishers, and bookstores. These literary gatekeepers typically ignore romance, fantasy, mystery, science fiction, young adult fiction, comic books, and …

Electrochemical Studies Of Cerium And Uranium In Licl-Kcl Eutectic For Fundamentals Of Pyroprocessing Technology, Dalsung Yoon

Theses and Dissertations

Understanding the characteristics of special nuclear materials in LiCl-KCl eutectic salt is extremely important in terms of effective system operation and material accountability for safeguarding pyroprocessing technology. By considering that uranium (U) is the most abundant and important element in the used nuclear fuel, measurements and analyses of U properties were performed in LiCl-KCl eutectic salt. Therefore, the electrochemical techniques such as cyclic voltammetry (CV), open circuit potential (OCP), Tafel, linear polarization (LP), and electrochemical impedance spectroscopy (EIS) were conducted under different experimental conditions to explore the electrochemical, thermodynamic, and kinetic properties of U in LiCl-KCl eutectic. The ultimate goal …

Nostalgia And Iphone Camera Apps: An Ethnographic Visual Approach To Iphoneography, Maria L. De Panbehchi

Theses and Dissertations

The iPhone is the most popular smartphone and camera on social media. iPhoneography, the photography taken or edited with the iPhone, has set the trend of nostalgic photography on social media during the 2010s; thus, the iPhone, a high-tech camera, produces low-tech-looking images. This dissertation attempts to find out why iPhone photographers (iPhoneographers) take, edit, and share images that mimic photographs taken with analog photographic equipment. I argue that nostalgia allows iPhoneographers to use the iPhone as a creative tool and to belong to a community. Based on the arguments of Vilém Flusser—who suggested that photographers are more interested in …

Beyond The Single Story: How Analog Hypertext Facilitates Representation Of Multiple Critical Perspectives In An Art Museum Object Study Gallery, Aimee D. Hunt

Theses and Dissertations

This project utilized a form of arts based educational research described as analog hypertext to develop interpretative material representing multiple critical, theoretical, and disciplinary perspectives on objects in a university art museum’s object study gallery. Drawing on scholars’ recommendations for postcolonial interpretation of non-Western art, the project created a web of information, which simultaneously revealed and critiqued the underlying ideologies and power structures shaping the museum’s display in an effort to change existing interpretive practice. The project developed five color-coded thematic self-guided tours—art as commodity, spiritual practice, technology and cultural evolutionism, mortuary rituals, and postcolonial perspectives—presented to the public as …

Wreading, Performing, And Reflecting: The Application Of Narrative Hypertext And Virtual World Experiences To Social Work Education, Linda Ayscue Gupta Phd

Theses and Dissertations

In this dissertation I propose the use of a new media composition of narrative hypertext, performances in a virtual world, and a dialogic process of writing to provide a continuum of learning opportunities in social work education. I suggest that the structure of the hypertext narrative, embedded with hypermedia, mirrors the dissociative aspects of traumatic memory. I argue that work with the multivocality and multisequentiality of narrative hypertext emulates the process of discovery in the clinical interview. The immersive component of work in a virtual world deepens the realism and affective impact of simulations and creates opportunities to practice and …