Digital Commons Network^™

Inquiry Of Lipid Membranes Interacting With Functional Peptides And Polyphenol Drug Molecules, Chian Sing Ho

USF Tampa Graduate Theses and Dissertations

Cellular membranes are important targets for many membrane-active peptides and drug compounds. Here we are interested in deciphering how lipid membranes are perturbed by several membrane-active molecules, including the transmembrane domain of the influenza M2 protein (M2TM), aggregates formed by a synthetic polyglutamine peptide, and three polyphenol compounds (i.e., tamoxifen, genistein, and verapamil). We employ phase-separated ternary lipid model membranes in the form of giant unilamellar vesicles (GUVs) to simulate raft-like structures that have been proposed to govern many important processes in plasma membranes (e.g., intracellular singling and trafficking). Specifically, we use fluorescent microscopy to interrogate how those membrane additives …

Intersections Of Violence Against Women And Health: Implications For Health Law And Policy In Nigeria, Cheluchi Onyemelukwe

William & Mary Journal of Race, Gender, and Social Justice

No abstract provided.

Volume 53, Number 45: June 22, 2016, University Of North Dakota

University Letter Archive

No abstract provided.

Komorbiditas Diabetes Mellitus Terhadap Manifestasi Klinik Dan Kualitas Hidup Pada Penderita Tuberkulosis Paru, Andi Selvi Yusnitasari, Ida Leida M.Thaha, Muhammad Syafar

Media Kesehatan Masyarakat Indonesia

Komorbiditas diabetes mellitus dapat menjadi beban ganda (double burden) dalam penyelesaian penyakit menular dan kronik, hal ini terjadi karena TB dan DM berinteraksi satu sama lain yang dapat menyebabkan pelaksanaan pengobatan klinis menjadi lebih sulit dibandingkan dengan pengobatan pada penyakit tunggal TB atau DM. Penelitian ini bertujuan menganalisis besar risiko diabetes mellitus terhadap manifestasi klinikdan kualitas hidup pada penderita TB-DM dan TB tanpa DM. Jenis penelitian yang digunakan adalah observasional analitik dengan rancangan prospectif cohort study. Total sampel sebanyak 60 orang terdiri dari, 30 orang TB-DM dan 30 orang TB tanpa DM. Penarikan sampel dilakukan dengan purposive sampling. Data dianalisis …

Urgensi Perubahan Implementasi Kebijakan Dalam Menurunkan Ims, Hiv Dan Aids Pada Komunitas Lsl Di Kabupaten Jember, Dewi Rokhmah, Khoiron Khoiron

Media Kesehatan Masyarakat Indonesia

Program penanggulangan AIDS populasi LSL selama ini telah menerapkan berbagai metode untuk merubah perilaku yang berisiko tinggi. Namun prevalensi HIV pada populasi LSL tetap saja naik. Penelitian ini bertujuan untuk menganalisis urgensi perubahan Stategi Kebijakan dalam MenurunkanIMS dan HIV dan AIDS pada Komunitas LSL Di Kabupaten Jember. Penelitian ini merupakan penelitian gabungan antara kuantitatif secara survei analitik dengan kualitatif melalui indept interview. Survei dilakukan melalui purposive sampling pada 89 LSL, kemudian data dianalisa menggunakan regeresi logistic. Data kualitatif dianalisis secara tematic content analysis. Hasil penelitian menunjukkan bahwa pendekatan Program PMTS melalui sosialisasi save sex pada komunitas LSL tidak efektif dalam …

Cyclic Di-Gmp-Regulated Periplasmic Proteolysis Of A Pseudomonas Aeruginosa Type Vb Secretion System Substrate, Richard B. Cooley, T. Jarrod Smith, Wilfred Leung, Valerie Tierney, Bradley Borlee, George A. O'Toole, Holger Sondermann

Dartmouth Scholarship

We previously identified a second-messenger-regulated signaling system in the environmental bacterium Pseudomonas fluorescens which controls biofilm formation in response to levels of environmental inorganic phosphate. This system contains the transmembrane cyclic di-GMP (c-di-GMP) receptor LapD and the periplasmic protease LapG. LapD regulates LapG and controls the ability of this protease to process a large cell surface adhesin protein, LapA. While LapDG orthologs can be identified in diverse

Diverse Impacts Of The Rs58542926 E167k Variant In Tm6sf2 On Viral And Metabolic Liver Disease Phenotypes, Mohammed Eslam, Alessandra Mangia, Thomas Berg, Henry Lik Yuen Chan, William L. Irving, Gregory J. Dore, Maria Lorena Abate, Elisabetta Bugianesi, Leon A. Adams, Mustafa A.M. Najim, Luca Miele, Martin Weltman, Lindsay Mollison, Wendy Cheng, Stephen Riordan, Janett Fischer, Manuel Romero-Gomez, Ulrich Spengler, Jacob Nattermann, Antony Rahme, David Sheridan, David R. Booth, Duncan Mcleod, Elizabeth Powell, Christopher Liddle, Mark W. Douglas, Der Poorten D Van, Jacob George

Peninsula Medical School

No abstract provided.

The Battle Against Antimicrobial Drug Resistance: Analyzing Recent Developments And The Necessity For Major Agricultural Reforms, Nikki Sanford

William & Mary Environmental Law and Policy Review

No abstract provided.

Saving The Orange: How To Fight Citrus Greening Disease (And It’S Not Through Genetic Engineering), Evan Feely

William & Mary Environmental Law and Policy Review

No abstract provided.

Staphylococcus Aureus Coordinates Leukocidin Expression And Pathogenesis By Sensing Metabolic Fluxes Via Rpirc, Divya Balasubramanian, Elizabeth A Ohneck, Jessica Chapman, Andy Weiss, Min Kyung Kim, Tamara Reyes-Robles, Judy Zhong, Lindsey N. Shaw, Desmond S. Lun, Beatrix Ueberheide, Bo Shopsin, Victor J Torres

Molecular Biosciences Faculty Publications

Staphylococcus aureus is a formidable human pathogen that uses secreted cytolytic factors to injure immune cells and promote infection of its host. Of these proteins, the bicomponent family of pore-forming leukocidins play critical roles in S. aureus pathogenesis. The regulatory mechanisms governing the expression of these toxins are incompletely defined. In this work, we performed a screen to identify transcriptional regulators involved in leukocidin expression in S. aureus strain USA300. We discovered that a metabolic sensor-regulator, RpiRc, is a potent and selective repressor of two leukocidins, LukED and LukSF-PV. Whole-genome transcriptomics, S. aureus exoprotein proteomics, and metabolomic analyses revealed that …

A Randomized, Double-Blind, Placebo-Controlled Phase Ii Trial Investigating The Safety And Immunogenicity Of Modified Vaccinia Ankara Smallpox Vaccine (Mva-Bn®) In 56-80-Year-Old Subjects, Richard N. Greenberg, Christine M. Hay, Jack T. Stapleton, Thomas C. Marbury, Eva Wagner, Eva Kreitmeir, Siegfried Röesch, Alfred Von Krempelhuber, Philip Young, Richard Nichols, Thomas P. Meyer, Darja Schmidt, Josef Weigl, Garth Virgin, Nathaly Arndtz-Wiedemann, Paul Chaplin

Internal Medicine Faculty Publications

Background Modified Vaccinia Ankara MVA-BN^® is a live, highly attenuated, viral vaccine under advanced development as a non-replicating smallpox vaccine. In this Phase II trial, the safety and immunogenicity of Modified Vaccinia Ankara MVA-BN^® (MVA) was assessed in a 56–80 years old population.

Methods MVA with a virus titer of 1 x 10⁸ TCID₅₀/dose was administered via subcutaneous injection to 56–80 year old vaccinia-experienced subjects (N = 120). Subjects received either two injections of MVA (MM group) or one injection of Placebo and one injection of MVA (PM group) four weeks apart. Safety was evaluated …

Determinan Sosial Dan Keteraturan Berobat Terhadap Perubahan Konversi Pasien Tuberkulosis Paru, Ummi Kalsum Supardi, Ida Leida M.Thaha, Rismayanti Rismayanti

Media Kesehatan Masyarakat Indonesia

Tuberkulosis paru merupakan penyakit menular yang masih menjadi masalah sosial kesehatan masyarakat, khususnya di Indonesia. Tahun 2012 terdapat perubahan konversi (12%) dan meningkat (15%) tahun 2013 pasien di Rumah Sakit Umum Labuang Baji Makassar dan BBKPM Kota Makassar. Penelitian ini bertujuan mengetahui besar faktor risiko perubahan konversi pasien TB paru berdasarkan pengetahuan, pendidikan, kondisi ekonomi, dan keteraturan berobat. Jenis penelitian desain case control study. Responden pada penelitian ini adalah penderita TB yang BTA (+) yang menjalani masa pengobatan 2 bulan, sebanyak 111 responden dengan perbandingan kasus dan kontrol 1 : 2. Analisis bivariat menggunakan uji kemaknaan Odds Ratio 95% CI. …

Ada News - 06/20/2016, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

In U.S. First, Uab Physician Uses Telehealth To Replace Comprehensive Face-To-Face Visit For Home Dialysis Patient, Tyler Greer

Browse All News

No abstract provided.

Week Of June 20, 2016, New York Medical College

InTouch

• Victor T. Madubuko Is Named Diversity and Inclusion Officer
• Department of Family and Community Medicine Hosts Annual Faculty Appreciation Dinner
• NYMC Honors Preceptors and Seminar Leaders at Annual Dinner
• School of Medicine Joins Ranks of AAMC Te4Q Sites

Novel Partitivirus Infection Of Bat White-Nose Syndrome (Wns) Fungal Pathogen Pseudogymnoascus Destructans Links Eurasian And North American Isolates, Ping Ren, Sunanda S. Rajkumar

KIP Articles

Bat White-nose Syndrome (WNS) fungus Pseudogymnoascus destructans had caused mass mortality in the North American bats. A single clone of the pathogen (Hap_1) was likely introduced in the United States while Eurasian population comprised of several haplotypes. The origin and spread of P. destructans remain enigmatic due in part to a lack of precise population markers. We searched for P. destructans mycoviruses as they are highly host-specific, and their spread could provide a window on the origin of the host fungus. We discovered a P. destructans bipartite virus PdPV-1 with two double-stranded RNA (dsRNA) segments - LS (1,683 bp) and …

Expansion Of Myeloid-Derived Suppressor Cells Promotes Differentiation Of Regulatory T Cells In Hiv-1+ Individuals, Ling Wang, Juan Zhao, Junping P. Ren, Xiao Y. Wu, Zheng D. Morrison, Mohamed A. El Gazzar, Shunbin Ning, Jonathan P. Moorman, Zhi Q. Yao

ETSU Faculty Works

Objective: Regulatory T cells (Tregs) contribute to HIV-1 disease progression by impairing antiviral immunity; however, the precise mechanisms responsible for the development of Tregs in the setting of HIV-1 infection are incompletely understood.

Design: In this study, we provide evidence that HIV-induced expansion of monocytic myeloid-derived suppressor cells (M-MDSCs) promote the differentiation of Foxp3+ Tregs.

Methods: We measured MDSC induction and cytokine expression by flow cytometry and analyzed their functions by coculturing experiments.

Results: We observed a dramatic increase in M-MDSC frequencies in the peripheral blood of HIV-1 seropositive (HIV-1+) individuals, even in those on antiretroviral therapy with undetectable viremia, …

Increasing Hiv-1 Pretreatment Drug Resistance Among Antiretroviral-Naïve Adults Initiating Treatment Between 2006 And 2014 In Nairobi, Kenya, Michael Chung, Silverman Rachel, Beck Ingrid, Yatich Nelly, Dross Sandra, Jennifer Mckernan-Mullin, Stephen Bii, Kenneth Tapia, Joshua Stern, Chohan Bhavna, Samah R. Sakr, James N. Kiarie

Internal Medicine, East Africa

Antiretroviral-naïve adults initiating antiretroviral therapy in Nairobi, Kenya were tested for HIV-1 drug resistance at codons K103N, Y181C, G190A, M184V, and K65R using an oligonucleotide ligation assay. Prevalence of pretreatment drug resistance increased from 3.89% in 2006 to 10.93% in 2014 (P < 0.001), and 95% of those with resistance had at least one nonnucleoside reverse transcriptase inhibitor mutation. Resistance to tenofovir (K65R) was found in 2014 but not in 2006.

Comprehensive Refugee Health Surveillance In Philadelphia: A Combined Resettlement And Clinical Patient Registry, Colleen Payton, Mph, Ches, Brittany Divito, Mph, Bsn, Rn, Gretchen Shanfeld, Mph, Zoe Agoos, Md, Kevin Scott, Md, Marc Altshuler, Md

Department of Family & Community Medicine Presentations and Grand Rounds

Oral presentation at the 6^th Annual North American Refugee Health Conference, Niagara Falls, New York.

Goal:

To improve the success of refugee resettlement and health outcomes in Philadelphia by establishing a multi-agency registry with social services and clinical measures.

A Propensity-Matched Study Of The Association Of Contrast Media Administration With Acute Kidney Injury (Aki) In Icu Patients, Lisa-Mae Williams

All Publications

Background: Acute Kidney Injury (AKI) is associated with poor outcomes and increased costs in hospitalized patients. A recent meta analysis of controlled studies found no increased risk of AKI attributable to the use of radiocontrast media for diagnostic studies in hospitalized patients. This retrospective observational study provides a real world assessment of the risk of AKI in critically ill patients at BHSF with respect to contrast and other factors.

Methods: Data including patient demographics, physiological parameters, diagnoses and APACHE score were obtained from multiple sources in the BHSF data warehouse. Study data comprised all hospitalizations between 2010 and …

Pharmacologic Inhibition Of Host Phosphodiesterase-4 Improves Isoniazid-Mediated Clearance Of Mycobacterium Tuberculosis, Selvakumar Subbian, Mi-Sun Koo, Liana Tsenova, Vikram Khetani, Jerome B. Zeldis, Dorothy Fallows, Gilla Kaplan

Publications and Research

The lengthy duration of multidrug therapy needed to cure tuberculosis (TB) poses significant challenges for global control of the disease. Moreover, chronic inflammation associated with TB leads to pulmonary damage that can remain even after successful cure. Thus, there is a great need for the development of effective shorter drug regimens to improve clinical outcome and strengthen TB control. Host-directed therapy (HDT) is emerging as a novel adjunctive strategy to enhance the efficacy and shorten the duration of TB treatment. Previously, we showed that the administration of CC-3052, a phosphodiesterase-4 inhibitor (PDE4i), reduced the host inflammatory response during Mycobacterium tuberculosis …

Vaccination As A Tool To Reduce Antimicrobial Resistance Worldwide, Bernard Vallat, Elisabeth Erlacher

Vaccine Technology VI

Antimicrobial resistance is a global human and animal health concern that is influenced by the non-appropriate use of antimicrobial agents in both human and veterinary medicine, as well as in the plant sector. To combat antimicrobial resistance, the World Organisation for Animal health (OIE) has developed science-based intergovernmental standards and guidelines covering terrestrial animals and aquaculture.

The OIE also contributed to the development of the WHO Global Action Plan on Antimicrobial Resistance, adopted in 2015 by the World Health Assembly. The 180 Member Countries of the OIE expressed their support to this plan of action through a Resolution, …

How To Deliver New Vaccines Under Very Short Timelines: The Zapi Project, Jean-Christophe Audonnet

Vaccine Technology VI

The ZAPI project is the first One Health project in the European Innovative Medicine Initiative (IMI) program. Its objective is to define and validate unique methodologies that can be applied when new zoonotic infectious diseases will occur in Europe or other regions of the world. The project is supporting an entirely new approach for “anticipating the unexpected”, and is defining new ways to alleviate the technical constraints which are typically encountered with the classical vaccine development processes.

The ZAPI project involves a total of 20 partners, from academic, biotech, and industry origin. One important feature of ZAPI is that it …

Developing A Video-Based Ehealth Intervention For Hiv-Positive Gay, Bisexual, And Other Men Who Have Sex With Men: Study Protocol For A Randomized Controlled Trial, Sabina Hirshfield, Martin J. Downing Jr., Jeffrey T. Parsons, Christina Grov, Rachel J. Gordon, Steven T. Houang, Roberta Scheinmann, Patrick S. Sullivan, Irene S. Yoon, Ian Anderson, Mary Ann Chiasson

Publications and Research

Background: Gay, bisexual, and other men who have sex with men (GBMSM) accounted for 67% of new US human immunodeficiency virus (HIV) infections in 2012; however, less than 40% of HIV-positive GBMSM are virally suppressed. Preventing transmission from virally unsuppressed men who have condomless anal sex (CAS) with serodiscordant partners is a public health imperative. New HIV infections in GBMSM are attributed in part to online access to sex partners; therefore, low-cost eHealth interventions are a unique opportunity to reach men where they meet partners.

Objective: To describe the protocol of a randomized controlled trial evaluating whether video-based messaging delivered …

Elimination Of Taenia Solium Transmission In Northern Peru, Hector H. Garcia, Armando E. Gonzalez, Victor C.W. Tsang, Seth E. O'Neal, Fernando Llanos‑Zavalaga, Guillermo Gonzalvez, Jaime Romero, Silvia Rodriguez, Luz M. Moyano, Viterbo Ayvar, Andre Diaz, Allen Hightower, Philip S. Craig, Marshall W. Lightowlers, Charles G. Gauci, Elli Leontsini, Robert H. Gilman

OHSU-PSU School of Public Health Faculty Publications and Presentations

Background: Taeniasis and cysticercosis are major causes of seizures and epilepsy. Infection by the causative parasite Taenia solium requires transmission between humans and pigs. The disease is considered to be eradicable, but data on attempts at regional elimination are lacking. We conducted a three-phase control program in Tumbes, Peru, to determine whether regional elimination would be feasible.

Methods: We systematically tested and compared elimination strategies to show the feasibility of interrupting the transmission of T. solium infection in a region of highly endemic disease in Peru. In phase 1, we assessed the effectiveness and feasibility of six intervention …

Research Experience Leads Alumna To Residency Program, Sarah Gardner, Dave Albee

Press Releases

Motivated by the learning environment at Dominican, Natalie Aquino ’12 earned her doctorate of pharmacy last month at the University of California San Francisco. Now she is matched to the UC Davis Medical Center (PGY-1) acute care pharmacy residency program that will start this summer.

Temperature-Dependent Pathogenicity Of Grouper Iridovirus Of Taiwan (Tgiv), Hsin-I Liu, Ying-Chu Lin, Pinwen Peter Chiou, Hsin-Yiu Chou

Journal of Marine Science and Technology–Taiwan

Grouper iridovirus of Taiwan (TGIV) is one of the most devastating infectious pathogens of grouper fry in Taiwan. As environmental temperature often plays an important role in the outbreak of diseases, we assayed the impact of different temperatures (18, 25 and 32℃) on TGIV infection both in vitro and in vivo. Cytopathic effect (CPE) characterized as cell rounding and lysis were observed in TGIV-infected grouper SB (swim bladder) cells at 25℃ and 32℃, but was absent at 18℃. With temperature rising to 32℃, the presence of pycnotic nuclei and chromatin margination became prominent in the infected cells, indicating an apoptotic …

Data Correlations Between Gender, Cytomegalovirus Infection And T Cells, Nk Cells, And Soluble Immune Mediators In Elderly Humans, Ahmad Al-Attar, Steven R. Presnell, Charlotte A. Peterson, D. Travis Thomas, Charles T. Lutz

Pathology and Laboratory Medicine Faculty Publications

We describe a cohort of 50 elderly subjects, age at least 70 years. We present gender-specific findings in T lymphocyte markers and soluble immune mediators. We show the correlation between cytomegalovirus infection status with CD56^dim NK cell responses to a variety of stimuli and with CD56^bright/CD56^dim NK cell ratio. We also present the correlation of retinol binding protein (RBP)−4 plasma levels with NK cell responses and we explore the relationship between gender and adiponectin, 25(OH)D (vitamin D), and RBP4 in affecting CD56^dim NK cell responses. These data are discussed in Al-Attar et al. (2016)

Comparing An Std Education Program To Hiv Screening For African American Adolescents At An Urban High School, Sharron Y. Hightower

DNP and Student Works

Globally, human immunodeficiency virus (HIV), acquired immunodeficiency diseases (AIDS) and sexual transmitted diseases (STDs) are pressing issues that need evidenced-based interventions.? Data show that African-American adolescents are a higher rate than any other ethnic group. To address this public health problem, it is imperative to incorporate strategies for adolescent HIV/STD education prevention programs. The purpose of this project was to examine the relationship between a HIV education intervention program and participation in HIV and STD screening with 132 African American students in grades 9 through 12 at an urban high school. The project employed quantitative data from the Coordinated School …

Engaging Rural Georgians In The Internal Revenue Service (Irs)-Mandated Community Health Needs Assessment, Raymona H. Lawrence, Dziyana Nazaruk, Marie Denis-Luque, Stuart H. Tedders

Epidemiology: Faculty Publications (2002-2018)

Background: On March 23, 2010, President Barack Obama signed the Patient Protection and Affordable Care Act (ACA) into law. The law became effective on March 23, 2012. The ACA stipulates that non-profit hospitals must demonstrate benefit to their communities through the process of community health needs assessments (CHNAs). Failure to comply with this law may result in loss of non-profit status or large fines. This report describes strategies for engaging rural communities in Internal Revenue Service (IRS)-mandated CHNAs.

Methods: Public health practitioners from Georgia Southern University’s Jiann Ping Hsu College of Public Health collaborated with 18 rural, non-profit hospitals to …