Digital Commons Network^™

Metabolic Requirements Of Escherichia Coli In Intracellular Bacterial Communities During Urinary Tract Infection Pathogenesis, Matt S. Conover, Maria Hadjifrangiskou, Joseph J. Palermo, Michael E. Hibbing, Karen W. Dodson, Scott J. Hultgren

Open Access Publications

No abstract provided.

Hyperexpansion Of Rna Bacteriophage Diversity, Siddharth R. Krishnamurthy, Andrew B. Janowski, Guoyan Zhao, Dan Barouch, David Wang

Open Access Publications

No abstract provided.

Interleukin-17a Contributes To The Control Of Streptococcus Pyogenes Colonization And Inflammation Of The Female Genital Tract, Alison J. Carey, Jason B. Weinberg, Suzanne R. Dawid, Carola Venturini, Alfred K. Lam, Victor Nizet, Michael G. Caparon, Mark J. Walker, Michael E. Watson

Open Access Publications

No abstract provided.

Impact Of Time To Appropriate Therapy On Mortality In Patients With Vancomycin-Intermediate Staphylococcus Aureus Infection, Jason P. Burnham, Carey-Ann D. Burnham, David K. Warren, Marin H. Kollef

Open Access Publications

No abstract provided.

Cardiomyocyte-Specific Ablation Of Med1 Subunit Of The Mediator Complex Causes Lethal Dilated Cardiomyopathy In Mice, Yuzhi Jia, Hsiang-Chun Chang, Matthew J. Schipma, Jing Liu, Varsha Shete, Ning Liu, Tatsuya Sato, Edward B. Thorp, Philip M. Barger, Yi-Jun Zhu, Navin Viswakarma, Yashpal S. Kanwar, Hossein Ardehali, Bayar Thimmapaya, Janardan K. Reddy

Open Access Publications

No abstract provided.

Culture Of Urine Specimens By Use Of Chromid Cps Elite Medium Can Expedite Escherichia Coli Identification And Reduce Hands-On Time In The Clinical Laboratory, Melanie L. Yarbrough, Meghan A. Wallace, Cynthia Marshall, Erin Mathias, Carey-Ann D. Burnham

Open Access Publications

No abstract provided.

An Evaluation Of Food As A Potential Source For Clostridium Difficile Acquisition In Hospitalized Patients, Jennie H. Kwon, Cristina Lanzas, Kimberly A. Reske, Tiffany Hink, Sondra M. Seiler, Kerry M. Bommarito, Carey-Ann D. Burnham, Erik R. Dubberke

Open Access Publications

No abstract provided.

Presence Of Pathogenic Escherichia Coli Is Correlated With Bacterial Community Diversity And Composition On Pre-Harvest Cattle Hides, Jessica Chopyk, Ryan M. Moore, Zachary Dispirito, Zachary R. Stromberg, Gentry L. Lewis, David G. Renter, Natalia Cernicchiaro, Rodney A. Moxley, K. Eric Wommack

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Since 1982, specific serotypes of Shiga toxin-producing Escherichia coli (STEC) have been recognized as significant foodborne pathogens acquired from contaminated beef and, more recently, other food products. Cattle are the major reservoir hosts of these organisms, and while there have been advancements in food safety practices and industry standards, STEC still remains prevalent within beef cattle operations with cattle hides implicated as major sources of carcass contamination. To investigate whether the composition of hide-specific microbial communities are associated with STEC prevalence, 16S ribosomal RNA (rRNA) bacterial community profiles were obtained from hide and fecal samples collected from a large …

Prevalence And Level Of Enterohemorrhagic Escherichia Coli In Culled Dairy Cows At Harvest, Zachary R. Stromberg, Gentry L. Lewis, Sharif S. Aly, Terry W. Lehenbauer, Joseph M. Bosilevac, Natalia Cernicchiaro, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

The primary objective of this study was to determine the prevalence and level of enterohemorrhagic Escherichia coli (EHEC) O26, O45, O103, O111, O121, and O145 (collectively EHEC-6) plus EHEC O157 in fecal, hide, and preintervention carcass surface samples from culled dairy cows. Matched samples (n=300) were collected from 100 cows at harvest and tested by a culture-based method and two molecular methods: NeoSEEK STEC (NS) and Atlas STEC EG2 Combo. Both the culture and NS methods can be used to discriminate among the seven EHEC types (EHEC-7), from which the cumulative prevalence was inferred, whereas the Atlas method can discriminate …

Recapitulating Cross-Species Transmission Of Sivcpz To Humans Using Humanized-Blt Mice, Zhe Yuan, Guobin Kang, Fangrui Ma, Wuxun Lu, Wenjin Fan, Christine M. Fennessey, Brandon F. Keele, Qingsheng Li

Nebraska Center for Virology: Faculty Publications

The origins of HIV-1 have been widely accepted to be the consequence of simian immunodeficiency viruses from wild chimpanzees (SIVcpz) crossing over to humans. However, there has not been any in vivo study of SIVcpz infection of humans. Also, it remains largely unknown why only specific SIVcpz strains have achieved cross-species transmission and what transmission risk might exist for those SIVcpz strains that have not been found to infect humans. Closing this knowledge gap is essential for better understanding cross-species transmission and predicting the likelihood of additional cross-species transmissions of SIV into humans. Here we show hu-BLT mice are susceptible …

High Glucose Induces Reactivation Of Latent Kaposi’S Sarcoma-Associated Herpesvirus, Fengchun Ye, Yan Zeng, Jingfeng Sha, Tiffany Jones, Kurt Kuhne, Charles Wood, Shou-Jiang Gao

Nebraska Center for Virology: Faculty Publications

High prevalence of Kaposi’s sarcoma (KS) is seen in diabetic patients. It is unknown if the physiological condition of diabetes contributes to KS development. We found elevated levels of viral lytic gene expression when Kaposi’s sarcoma-associated herpesvirus (KSHV) infected cells were cultured in high glucose medium. To demonstrate the association between high glucose and KSHV replication, we xeno29

grafted telomerase-immortalized human umbilical vein endothelial cells that are infected with KSHV (TIVE-KSHV) into hyperglycemic and normal nude mice. The injected cells expressed significantly higher levels of KSHV lytic genes in hyperglycemic mice than in normal mice. We further demonstrated that high …

Persistent Low-Level Replication Of Sivδnef Drives Maturation Of Antibody And Cd8 T Cell Responses To Induce Protective Immunity Against Vaginal Siv Infection, Sama Adnan, R. Keith Reeves, Jacqueline Gillis, Fay E. Wong, Yi Yu, Jeremy V. Camp, Qingsheng Li, Michelle Connole, Yuan Li, Michael Piatak Jr., Jeffrey D. Lifson, Wenjun Li, Brandon F. Keele, Pamela A. Kozlowski, Ronald C. Desrosiers, Ashley T. Haase, R. Paul Johnson

Nebraska Center for Virology: Faculty Publications

Defining the correlates of immune protection conferred by SIVΔnef, the most effective vaccine against SIV challenge, could enable the design of a protective vaccine against HIV infection. Here we provide a comprehensive assessment of immune responses that protect against SIV infection through detailed analyses of cellular and humoral immune responses in the blood and tissues of rhesus macaques vaccinated with SIVΔnef and then vaginally challenged with wild-type SIV. Despite the presence of robust cellular immune responses, animals at 5 weeks after vaccination displayed only transient viral suppression of challenge virus, whereas all macaques challenged at weeks 20 and 40 post-SIVΔnef …

Health Care Worker Follow-Up Compliance After Occupational Bloodborne Pathogens Exposure: A Brief Report, Juan C. Díaz, Lucas A. Johnson

Uniformed Services University of the Health Sciences

A retrospective cohort study was conducted examining health care worker (HCW) compliance with Centers for Disease Control and Prevention recommendations following occupational bloodborne pathogen (BBP) exposure. HCWs with a BBP exposure from a known HIV- or hepatitis C virus-seropositive individual were less likely to complete recommended follow-up compared with HCWs with seronegative source patient exposures (adjusted odds ratio, 0.02 and 0.09, respectively). Continued targeted education and extra vigilance in performing postexposure surveillance are warranted in this higher-risk population.

Parental Presence At The Bedside Of A Child With Suspected Ebola: An Expert Discussion, Cynthia F. Hinton, H. Dele Davies, Susan N. Hocevar, Steven E. Krug, Aaron M. Milstone, Leonard Ortmann, Cynthia H. Cassell, Georgina Peacock, Stephanie E. Griese

United States Public Health Resources

The Ebola virus disease (Ebola) outbreak in West Africa (2014-2015) prompted domestic planning to address the scenario in which a traveler imports Ebola into the United States. Parental presence at the bedside of a child with suspected or confirmed Ebola emerged as a challenging issue for pediatric health care providers and public health practitioners. At the heart of the issue was the balance of family-centered care and appropriate infection control, which are not easily aligned in the setting of Ebola. In the following dialogue, pediatricians, who participated in discussions about parental presence during the evaluation of pediatric persons under investigation, …

Behavioral Modification And Relapse Rates In Opioid-Dependent Pregnant Women Managed With Subutex, Tammy Lynn Minor

Walden Dissertations and Doctoral Studies

Behavioral Modification and Relapse Rates in

Opioid-Dependent Pregnant Women Managed with Subutex

by

Tammy Minor

MSN, Walden University, 2009

BSN, Marshall University, 1986

Project Submitted in Partial Fulfillment

of the Requirements for the Degree of

Doctor of Nursing Practice

Walden University

March 2016

Opioid dependency affects not only the individual who is dependent on opioids but negatively impactsalso the family unit, the community, and society as a whole. Opioid use in the prenatal period can have devastating effects on both the mother and the fetus. The purpose of this paper is to perform a secondary analysis of the effectiveness of …

Uptake Of Nanoparticles By Vibrio Gazogenes, Shonda Renee Jones

Theses and Dissertations

Vibrio gazogenes, a Gram-negative species of marine bacteria, was capable of transporting 20 nm (diameter) surface-carboxylated, polystyrene, fluorescent FluoSpheres® microspheres (excitation/emission = 505/515) through the outer membrane, which was indicated by a detectable decrease in the fluorescence intensity of the nanoparticles in the culture medium. A mechanism of transport was investigated involving the ATP-binding cassette (ABC) protein transporters that traverse the outer membrane. Inhibition of the ABC transporters did not prevent the entry of the nanoparticles into the cell, suggesting there was an alternate mechanism of transport. The addition of nanoparticles to the culture medium also did not provide any …

Breastfeeding Among Working Mothers In Saudi Arabia, Maryam Suliman Alhabas

Theses and Dissertations

Objective: To examine the relationship of not having breastfeeding support policy in workplace such as employees pumping breast milk or breastfeeding their infant while at work policy to early initiation of formula and cessation of breastfeeding. Design: Crosssectional study. Setting and Subjects: A total of 273 workingwomen, working in 12 different organizations in Riyadh, KSA. Method: An electronic questionnaire survey was administered to 340 workingwomen in 12 different organizations in Riyadh. Questions were about participants’ demographics and employment characteristics, breastfeeding support services in their workplace, knowledge about breastfeeding benefits, breastfeeding duration after return to work, and formula initiation. Results: Having …

Flaviviruses As A Cause Of Undifferentiated Fever In Sindh Province, Pakistan: A Preliminary Report, Erum Khan, Joveria Q. Farooqi, Kelli L. Barr, Dhani Prakoso, Amna Nasir, Akbar Kanji, Sadia Shakoor, Faisal Riaz Malik, Rumina Hasan, John A. Lednicky

Department of Pathology and Laboratory Medicine

Arboviral diseases are expanding worldwide, yet global surveillance is often limited due to diplomatic and cultural barriers between nations. With human encroachment into new habitats, mosquito-borne viruses are also invading new areas. The actual prevalence of expanding arboviruses is unknown in Pakistan due to inappropriate diagnosis and poor testing for arboviral diseases. The primary objective of this study was to document evidence of flavivirus infections as the cause of undifferentiated fever in Pakistan. Through a cooperative effort between the USA and Pakistan, patient exposure to dengue virus (DENV), West Nile virus (WNV), and Japanese encephalitis virus (JEV) was examined in …

Decolonizing Revelation: A Spatial Reading Of The Blues, Rufus Burnett Jr.

Electronic Theses and Dissertations

Decolonizing Revelation: A Spatial Reading of the Blues demonstrates that the cultural phenomenon of the blues is an indigenous way of knowing that offsets the hidden logic of racialized dominance within modern Christian understandings of revelation. In distinction from the Christian, Religious, and racialized understandings of the blues, this dissertation focuses on the space in which the blues emerges, the Delta Region of the United States. By attending to space, this dissertation shows how critical consideration of geography and region can reveal nuances that are often veiled behind racialized and theologized ways of understanding the people of the Delta Region. …

Distribution Of Hepatitis C Virus Genotypes Among Intravenous Drug Users In The Çukurova Region Of Turkey, Enver Üçbi̇lek, Bahri̇ Abayli, Mahmut Bakir Koyuncu, Durdane Midikli, Süveyda Gözüküçük, Alper Akdağ, Osman Özdoğan, Engi̇n Altintaş, Orhan Sezgi̇n

Turkish Journal of Medical Sciences

Background/aim: The most common hepatitis C virus (HCV) genotype in Turkey is genotype 1. However, there has not been a study about the distribution of HCV genotypes among intravenous drug users (IVDUs) in the Çukurova region of Turkey. This study was planned to understand if there is a difference between IVDUs and the normal population. Materials and methods: Between May 2010 and May 2014, anti-HCV positive IVDUs who applied to the 6 hospitals in the Çukurova region of Turkey were included in this study. Their HCV genotypes were studied. Results: Ninety-seven anti-HCV positive IVDUs were screened in terms of HCV …

Antimicrobial Stewardship In Long-Term Care Facilities: A Call To Action, Haley J. Morrill, R. Caffrey, Robin L. P. Jump, David Dosa, Kerry L. Laplante

Pharmacy Practice and Clinical Research Faculty Publications

Antimicrobial resistance is a global public health crisis and a national security threat to the United States, as stated in an executive order signed by the president in September 2014. This crisis is a result of indiscriminant antimicrobial use, which promotes selection for resistant organisms, increases the risk of adverse drug events, and renders patients vulnerable to drug-resistant infections. Antimicrobial stewardship is a key measure to combat antimicrobial resistance and specifically seeks to do this by improving antimicrobial use. Antimicrobial stewardship compliments infection control practices and it is important to note that these 2 disciplines are distinct and cannot be …

The Effect Of Molecular Rapid Diagnostic Testing On Clinical Outcomes In Bloodstream Infections: A Systematic Review & Meta-Analysis, Tristan T. Timbrook, B. Morton, Kevin W. Mcconeghy, Aisling R. Caffrey, Eleftherios Mylonakis, Kerry L. Laplante

Pharmacy Practice and Clinical Research Faculty Publications

Background. Previous reports on molecular rapid diagnostic testing (mRDT) do not consistently demonstrate improved clinical outcomes in bloodstream infections (BSIs). This meta-analysis seeks to evaluate the impact of mRDT in improving clinical outcomes in BSIs.

Methods. We searched PubMed, CINAHL, Web of science, and EMBASE through May 2016 for BSI studies comparing clinical outcomes by mRDT and conventional microbiology methods.

Results. Thirty-one studies were included with 5,920 patients. Risk of morality was significantly lower with mRDT as compared to conventional microbiology methods (OR 0.66, 95% CI 0.54-0.80) yielding a NNT of 20. The risk of mortality was …

Enhancement Of Cancer Vaccine Efficacy Via Nanoparticle Or Molecular-Based Adjuvants, Myunggi An

Wayne State University Theses

Adjuvants are immunomodulators which enhance immune responses to vaccines. However, parenteral administration of unformulated adjuvants fails to reach lymph nodes (LNs), the anatomic organ where the primary functions of immune cells are orchestrated. The LN-targeting delivery plays the key roles in promoting immune activation and has the great potential to transform disease treatment. The main goal of this thesis is to develop efficient vaccine delivery systems to target therapeutics into draining lymph nodes (dLNs) for ensuring their immunostimulatory activity. We introduced therapeutic applications of activating TLR9 with synthetic CpG oligodeoxynucleotide (ODN) agonists in nanoparticle or molecular form to activate immune …

The Role Of Alarmins, Invariant Nkt Cells And Senescence In The Pathophysiology Of Sterile Intra-Amniotic Inflammation, Olesya Plazyo

Wayne State University Dissertations

Preterm birth is defined as the delivery of a live baby prior to the 37th week of gestation. It is the leading cause of neonatal mortality worldwide. Preterm neonates are at a higher risk for short- and long-term complications, and prematurity places significant burden on our society. Elucidation of the mechanisms that lead to spontaneous preterm labor will enable development of therapies to prevent this syndrome. We aimed to study pathological inflammation that is implicated in the pathophysiology of spontaneous preterm labor.

Pathological inflammation can be initiated by the activation of innate immunity either by microorganisms or alarmins, which are …

Effect Of Anthelminthic Treatment On Helminth Infection And Related Anaemia Among School-Age Children In Northwestern Ethiopia, Yonas Yimam, Abraham Degarege, Berhanu Erko

Journal Articles: Epidemiology

BACKGROUND: Information about improvements in the health status of population at-risk of helminth infection after anthelminthic treatment helps to evaluate the effectiveness of the large scale deworming program. The objectives of this study were to assess the impact of anthelminthic treatment on the prevalence and intensity of intestinal helminth infection, haemoglobin level and prevalence of anaemia among school-age children.

METHODS: A total of 403 children attending Tikur Wuha Elementary School in Jiga, northwestern Ethiopia were enrolled in this study between February and March 2011. Formol-ether concentration and Kato-Katz methods were used to examine stool for intestinal helminth infections at baseline …

Epidemiology Of Plasmodium And Helminth Coinfection And Possible Reasons For Heterogeneity, Abraham Degarege, Berhanu Erko

Journal Articles: Epidemiology

Understanding the impact of helminth infections on clinical malaria is useful for designing effective malaria control strategies. Plenty of epidemiological studies have been conducted to unravel the nature of interactions between Plasmodium and helminth infection. Careful broad summarization of the existing literature suggests that Schistosoma mansoni and hookworm infections may increase the risk of clinical malaria and associated morbidities, but Trichuris trichiura infection is not associated with the occurrence of clinical malaria and related outcomes. However, findings about effect of Ascaris lumbricoides and Schistosoma haematobium infection on clinical malaria are contradictory. Furthermore, the nature of relationship of helminth infection with …

Are You Pepped And Prepped For Travel? Risk Mitigation Of Hiv Infection For Travelers, David Brett-Major, P. T. Scott, T A. Crowell, C. S. Polyak, K. Modjarrad, M. L. Robb, D. L. Blazes

Journal Articles: Epidemiology

The HIV pandemic persists globally and travelers are at risk for infection by the Human Immunodeficiency Virus (HIV). While HIV-focused guidelines delineate risk stratification and mitigation strategies for people in their home communities, travel issues are not addressed. In this review, direct and indirect evidence on HIV risk among travelers is explored. The burgeoning practice of employing pre-exposure prophylaxis (PrEP) with anti-retroviral therapy in the non-travel setting is introduced, as well as the more established use of post-exposure prophylaxis (PEP). Challenges in applying these lessons to travelers are discussed, and a new guidelines process is scoped and recommended.

Assessing Post Operative Information Transfers: Evaluation Of Patient Outcomes, Monica Wynette Rose

MUSC Theses and Dissertations

Purpose: The purpose of this dissertation research was to study postoperative handover information transfers (PITS) and to ultimately improve patient safety and patient outcomes. One of the goals was to identify deficits in PITS by exploring information needs and processes related to PITS. Grounded by the social ecological model (SEM), a scoping review of extant literature was conducted to identify individual, interpersonal, organizational environmental and organizational policy level factors that influence the quality and processes of post-operative information transfers (PITS). An integrative review of extant literature was conducted to describe how PITS have been studied and to describe instruments that …

Fcrl As A Target For Immunotherapy Of B-Cell Lymphoma, Mollie Capone

MUSC Theses and Dissertations

Current treatments for B-cell lymphomas are largely non-specific and survival rates among those diagnosed with aggressive subtypes of non-Hodgkin’s lymphoma (NHL) remain poor. Thus, there is a demand for immune therapies that could reduce cyto-toxicity and improve outcomes. While B-cell lymphoma tumor cells express HLA class II molecules, there are multiple defects in functional HLA class II antigen (Ag) presentation to CD4+ T-cells. Studies in our laboratory suggest that in a subtype of non-Hodgkin’s lymphoma, Burkitt’s Lymphoma (BL) there are associated molecules that may disrupt CD4+ T cell recognition of malignant B-cells. To dissect the role of BL-associated molecules in …

Annual Report 2015-2016: Student Life & Development, Denise Mcdowell, Carl T. Stange, Kendra Weber, Alex Kromminga, Karen Johnson, Benedict Ezeoke, Jeff Reinardy, Paula Scheevel, Alex Hines, Mark Bambenek, Tracy Rahim, Gar Kellom, Joseph E. Reed, Barbara Oertel

Annual Reports

Student Life and Development (SLD) professionals at Winona State University (WSU) deliver programs, services, and activities that support students' academic achievement, social development, and well-being in the timely pursuit of their educational goals. The 2015-2016 SLD Annual Report provides information from the following departments: Admissions, Community Engagement, Conduct & Citizenship, Counseling & Wellness, Dean of Students, Fitness & Wellness, Health & Wellness Services, Housing & Residence Life, Inclusion & Diversity Office, Intramurals, Student Activities & Leadership, TRIO & Student Support Services, the Student Union, the Warrior Hub, and the Warrior Success Center.