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Role Of Microsatellite Markers In Molecular Population Genetics Of Fruit Flies With Emphasis On The Bactrocera Dorsalis Invasion Of Africa, Fathiya M. Khamis, Anna R. Malacrida

All Peer-Reviewed Publications

Microsatellites, also referred to as short tandem repeats (STRs) or simple sequence repeats (SSRs) are short sequences of tandem repeats of 1-6 bp in length in clusters of less than 150 bp flanked by sections of non-repetitive unique sequences that are scattered throughout the nuclear genome. These markers are co-dominant and hypervariable, revealing many alleles per locus; they are inherited in Mendelian fashion making them useful for detecting genetic variability within species. Once isolated and characterized, microsatellites can also be used in closely related taxa. Microsatellites can be amplified, even from highly degraded DNA, and are very simple to score. …

Fruit Fly Nutrition, Rearing And Quality Control, Samira A. Mohamed, Fathiya M. Khamis, Chrysantus M. Tanga

All Peer-Reviewed Publications

Tephritid fruit flies are recognized worldwide as an important threat to the horticultural industry. Most of the species belonging to this group are highly polyphagous attacking several important fruits and vegetables. They cause direct damage through larval feeding and indirect losses are associated with quarantine restrictions. The increasing awareness of the damage caused by these fruit flies to the horticultural industry has created a demand for the development of control measures based on integrated pest management (IPM) strategies and the sterile insect technique (SIT). However, success of the majority of these control methods largely depends on the ability to establish …

Maize Landraces Are Less Affected By Striga Hermonthica Relative To Hybrids In Western Kenya, Charles A.O. Midega, John Pickett, Antony Hooper, Jimmy Pittchar, Zeyaur R. Khan

All Peer-Reviewed Publications

Production of maize in western Kenya is severely constrained by the parasitic weed striga. Although productivity of maize can be improved through adoption of improved varieties, adoption of such varieties remains low in the region, as the majority of smallholder farmers still grow unimproved open-pollinated varieties (landraces). The performance of two improved hybrid varieties was evaluated against six landraces in striga-infested soils in western Kenya. The varieties were planted in plots under natural striga infestation and were supplemented with pot experiments under artificial infestation. Striga emergence was lower in landraces than in the hybrid varieties in both field and pot …

Spatial Separation Of Semiochemical Lurem-Tr And Entomopathogenic Fungi To Enhance Their Compatibility And Infectivity In An Autoinoculation System For Thrips Management, David K. Mfuti, Sevgan Subramanian, Rob Whm Van Tol, Gerrie L. Wiegers, Willem J. De Kogel, Saliou Niassy, Hannalene Du Plessis, Sunday Ekesi, Nguya K. Maniania

All Peer-Reviewed Publications

BACKGROUND: The effect of spatial separation of the semiochemical Lurem-TR, which has been found to inhibit conidia of entomopathogenic fungi when put together, on the persistence of conidia of Metarhizium brunneum and M. anisopliae was evaluated in the greenhouse and field in order to develop an autodissemination strategy for the management of Megalurothrips sjostedti on cowpea crop. Influence of spatial separation of the semiochemical on thrips attraction and conidial acquisition by thrips from the autoinoculation device was also investigated in the field. RESULTS: Persistence of conidia of M. brunneum and M. anisopliae increased with distance of separation of Lurem-TR. Direct …

Ratcheting And Energetic Aspects Of Synchronization In Coupled Bursting Neurons, E. Marius Yamakou, E. Maeva Inack, F. M. Moukam Kakmeni

All Peer-Reviewed Publications

In this paper, we investigate the dynamics of two coupled bursting neurons. The Hindmarsh–Rose mathematical model of the neuron subjected to an external periodic stimulus is considered. We transform this model into that of the one-dimensional problem of a particle driven by a periodic external force under the influence of a double-well potential. We study the bifurcation structures, chaotic behavior, and the synchronization dynamics of the transformed model. Numerical simulations reveal the existence of some bifurcation structures including saddle-node, symmetry-breaking, and period-doubling route to chaos. By varying the system’s parameters, we also find thresholds for which the system remains periodic. …

A "Clarion" Call For Embracing Ipe As The Status Quo For Preparing Health Professionals To Engage In Interprofessional Health Research, Kimberly Adams Tufts

Ellmer School of Nursing Faculty Publications

Complex health conditions and the social-economic determinants that contribute to disease and injury incidence, prevalence, and health inequalities require multifaceted evidence-based interventions that only interprofessional research teams who collaborate across traditional disciplinary lines can generate. Interprofessionally driven and derived research evidence is the method of du jour. Nonetheless as a whole, health professionals who are often members of interprofessional health research teams are products of educational systems wherein they were educated in disciplinary silos. Health professionals that learn about, with, from, each other during their foundational education will be better prepared to function as interprofessional research team members. With the …

The Effectiveness Of An Anesthesia Handoff Tool: An Electronic Health Record Application To Enhance Patient Safety, Karen Gillikin, Nathaniel Apatov

Ellmer School of Nursing Faculty Publications

Perioperative patient care handoffs are complex and multidimensional and require accurate attention to detail. Communication failures among healthcare providers increase the risk of morbidity and mortality. Utilizing a standardized handoff tool located within the electronic anesthesia record formalizes the handoff process and improves patient safety. Prior to the introduction of the Electronic Anesthesia Handoff Tool, 82 patient care transfer observations were conducted; subsequent to the launch of the tool, 75 patient care transfer observations were conducted, and then before and after comparisons were made. Descriptive statistics, a two-tailed t-test, and Spearman’s correlations were conducted. Alpha level was set at p < 0.05. There were significantly (p< .05) fewer errors made in all categories of patient information following the introduction of the Electronic Anesthesia Handoff Tool. Though there were trends towards more omissions occurring after 3:00 p.m., the difference in most patient information categories was not significant (p< .05). In addition, there were no differences in omissions related to the severity of patient co-morbidities based on patients’ American Society of Anesthesiologists physical status classification. This study provided information regarding the incidence of patient information inaccuracies and omissions during patient care transfer before and after implementation of an electronic patient care transfer tool.

Assent Described: Exploring Perspectives From The Inside, Rebecca D. Poston

Ellmer School of Nursing Faculty Publications

Purpose- The purpose of this study was to describe the informed consent and assent experience for oncology research from the perspective of the participants: adolescents, their parents, and their physician providers.

Design & Methods- This descriptive mixed-methods study included the pilot use of the Quality of Informed Consent Questionnaire (QuIC) with an adolescent population and semi-structured interviews with adolescents, their parents, and their physician providers within 48-72 hours of the informed consent and assent discussion for a pediatric oncology clinical trial and again 6-9 weeks later. Results: Adolescents and their parents scored considerably lower on part A of the QuIC …

Life In Jacob's Factory: Then & Now, James Burke, Rebecca De Bút, Paul Gogarty, Allison Kavanagh, Sarah-Anne Kennedy, Rebecca Jones

DIT Exhibitions

Using the Jacob’s building as its focal point, this exhibition highlights living conditions in Dublin in the period from the 1911 Census to the 1916 Rebellion, the role the building played in the Easter Rebellion, and the working conditions and lives of the young men and women working in Jacob’s factory during the period compared to those of a typical DIT student today.

Video

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Understanding The Problem Solving Approaches Of Special Educators Through The Lens Of Adaptive Expertise, Serra T. De Arment

Theses and Dissertations

The purpose of this research was to investigate special educators’ problem solving approaches through the lens of adaptive expertise. An explanatory sequential mixed methods design was used with participants of varying experience levels and teaching contexts from one Mid-Atlantic state. Participants responded to a researcher-developed survey about their orientations to problem solving (N = 162), then a purposive sample completed semi-structured interviews (N = 8). Following survey measure refinement and validation, quantitative data were analyzed through descriptive statistics, z-scores, correlation, and chi-square test of independence. Subsequently, qualitative data were analyzed through iterative cycles of hypothesis and open coding. …

Rage And Social Media: The Effect Of Social Media On Perceptions Of Racism, Stress Appraisal, And Anger Expression Among Young African American Adults, Morgan Maxwell

Theses and Dissertations

Recently, social media has become a sociopolitical hotbed for discussions of racism. However, no extant studies have questioned if social media use increases how often African Americans vicariously and/or personally experience discrimination in America. The current study sought to answer this question. By examining the relationships between social media use, general stress, race-related stress, and anger expression, and the mediating role of perceived racism, this study explored if frequent social media use influences young African American adults’: a) perceptions of racism, b) experiences with general and race-related stress, and/or c) expressions of anger. The current study conducted an online survey …

The Role Of N-Acetyl-L-Cysteine (Nac) As An Adjuvant To Opioid Treatment In Patients With Inadequately Controlled Chronic Neuropathic Pain, Thomas B. Moore

Theses and Dissertations

Introduction. While opioid medications are commonly prescribed for management of neuropathic pain (NP), long-term use has been associated with increased risk for overdose, drug interactions and addiction. New strategies are necessary to better manage chronic pain, thereby reducing need for opioid medications and their associated adverse consequences. N-acetyl-L-cysteine (NAC), an over-the-counter supplement, has shown promise in the treatment of psychiatric and addictive disorders. In addition, NAC has shown promise for reducing physiological signs of NP in laboratory rat models, prompting this study.

Purpose. The present study was an open-label clinical trial of NAC as an adjuvant to opioid treatment for …

Radioprotective Cerium Oxide Nanoparticles: Molecular Imaging Investigations Of Conps’ Pharmacokinetics, Efficacy, And Mechanisms Of Action, Philip R. Mcdonagh Iii

Theses and Dissertations

Cerium oxide nanoparticles (CONPs) are being investigated for several anti-oxidant applications in medicine. One of their most promising applications is as a radioprotective drug, an area of research in need due to the severe side effects from radiation therapy. In this work, the potential of CONPs as a radioprotective drug is examined using four criteria: favorable biodistribution/pharmacokinetics, low toxicity, ability to protect normal tissue from radiation damage, and lack of protection of tumor. The mechanisms of action of CONPs are also studied. Biodistribution was determined in radiolabeled CONPs with surface coatings including citrate, dextran T10-amine (DT10-NH₂), dextran T10-polyethylene …

Toward Epistemological Diversity In Stem-H Grantmaking: Grantors’ And Grantees’ Perspectives On Funding Indigenous Research, Programming, And Evaluation, Jessica C. Venable

Theses and Dissertations

Mainstream institutions have, historically, dismissed Indigenous worldviews, knowledges, and research approaches (Bowman-Farrell, 2015; Harrington & Pavel, 2013). However, in recent years, a literature has emerged articulating Indigenous research methodologies (IRMs), and their distinctiveness from Western, Eurocentric perspectives on inquiry (Denzin, Lincoln, & Smith, 2008; Kovach, 2009; Smith, 1999 & 2012; Wilson, 2008). This has coincided with increased need for IRM scholars and practitioners to secure extramural funds to support their activities. But questions remain as to how the U.S. federal grant making enterprise has accommodated Indigenous frameworks. This research explores synergies in the ways that grantees, grant makers, and other …

“I Just Need To Get Myself Supervised:” Exploring Transformative Learning In The Development Of Professionalism Among Physicians In The First Year Of Graduate Medical Education, Elizabeth P. Marlowe

Theses and Dissertations

The study explored the learning experiences of first-year resident physicians during the first year of graduate medical education. The experiences of four intern physicians in the first year of residency training at an urban academic health system provided the site for the research. An exploratory case study research design was employed to examine the learning experiences of these new physicians. A qualitative approach was used to analyze data from interviews and ethnographic observations. The findings of this research study provide evidence surrounding how and what these physician trainees learned regarding professionalism during the first year of residency training.

The findings …

The Influence Of Client-, Family-, And Therapist-Level Pretreatment Characteristics On Therapist Delivery Of Youth Psychotherapy Treatments, Adriana Rodriguez

Theses and Dissertations

The purpose of this exploratory study was to examine the extent to which pretreatment characteristics influence therapist treatment adherence by using data sampled from a randomized effectiveness trial and an efficacy study. Research suggests that youth-, family-, and therapist-level pretreatment characteristics influence therapist behavior; however, this area is underdeveloped as most studies have focused on externalizing problem areas, family-based approaches, and the use of parent or therapist report to assess for therapist adherence. To date, no research has examined this question with anxiety as the target problem, individual-focused CBT, and with observational therapist adherence data. An observational coding measure, Cognitive-Behavioral …

Discriminative Stimulus Properties Of Endogenous Cannabinoid Degradative Enzyme Inhibitors, Robert Owens Ii

Theses and Dissertations

Inhibition of fatty acid amide hydrolase (FAAH) or monoacylglycerol lipase (MAGL), the chief degradative enzymes of N-arachidonoyl ethanolamine (anandamide; AEA) and 2-arachidonoylglycerol (2-AG), respectively, elicits no or partial substitution for Δ⁹-tetrahydrocannabinol (THC) in drug discrimination procedures. However, combined inhibition of both enzymes fully substitutes for THC, as well as produces a full constellation of cannabimimetic effects. Because no published report to date have investigated whether an inhibitor of endocannabinoid hydrolysis will serve as a discriminative stimulus, the purpose of this doctoral dissertation was to investigate whether C57BL/6J mice would learn to discriminate SA-57 (4-[2-(4-Chlorophenyl)ethyl]-1-piperidinecarboxylic acid 2-(methylamino)-2-oxoethyl ester), a …

Irreversible Zinc Block Of The Swelling-Activated Chloride Current In Di Tnc1 Astrocytes, Noah Belkhayat

Theses and Dissertations

The swelling-activated chloride current, commonly referred to as ICl,swell, is an outwardly-rectifying anion current that plays an important role in cell volume regulation, among other capacities. Despite several decades of research, the molecular identity of the channel responsible for this chloride current remains controversial. Recent indications that key endogenous sulfhydryl groups are capable of modifying the current led us to assess the effects of several divalent cations, including zinc, on ICl,swell. Zinc is known to tightly associate with sulfhydryl groups such as in zinc finger proteins. We found that extracellular zinc irreversibly inhibited ICl,swell at a site downstream in the …

Probing Allosteric, Partial Inhibition Of Thrombin Using Novel Anticoagulants, Stephen S. Verespy Iii

Theses and Dissertations

Thrombin is the key protease that regulates hemostasis; the delicate balance between procoagulation and anticoagulation of blood. In clotting disorders, like deep vein thrombosis or pulmonary embolism, procoagulation is up-regulated, but propagation of clotting can be inhibited with drugs targeting the proteases involved, like thrombin. Such drugs however, have serious side effects (e.g., excessive bleeding) and some require monitoring during the course of treatment. The reason for these side effects is the mechanism by which the drugs’ act. The two major mechanisms are direct orthosteric and indirect allosteric inhibition, which will completely abolish the protease’s activity. Herein we sought an …

Provision Of Hospital-Based Palliative Care And The Impact On Organizational And Patient Outcomes, Marisa L. Roczen

Theses and Dissertations

Hospital-based palliative care services aim to streamline medical care for patients with chronic and potentially life-limiting illnesses by focusing on individual patient needs, efficient use of hospital resources, and providing guidance for patients, patients’ families and clinical providers toward making optimal decisions concerning a patient’s care. This study examined the nature of palliative care provision in U.S. hospitals and its impact on selected organizational and patient outcomes, including hospital costs, length of stay, in-hospital mortality, and transfer to hospice. Hospital costs and length of stay are viewed as important economic indicators. Specifically, lower hospital costs may increase a hospital’s profit …

High Affinity Block Of Icl,Swell By Thiol-Reactive Small Molecules, Sung H. Park

Theses and Dissertations

Ebselen (Ebs) is considered as a glutathione peroxidase (GPx) mimetic and primarily thought to function by scavenging intracellular reactive oxygen species (ROS). Previous to our work, Deng et al. (2010a) demonstrated complete block of I_Cl,swell with 15 microM Ebs following endothelin-1 (ET-1) induced activation of the current in cardiomyocytes. This block was presumed to take effect mainly via the quenching of ROS. Nonetheless, our work with DI TNC1 astrocytes strongly emphasizes that Ebs might function by an alternative mechanism based on its kinetic profile in blocking I_Cl,swell. Our experiments showed that 45 nM Ebs can fully block …

The Consequences Of Alcohol Mixed With Energy Drink (Amed) Use In College Students With Attention-Deficit/Hyperactivity Disorder (Adhd), Brooke A. Green

Theses and Dissertations

College students with ADHD, but especially those who consume alcohol, may be at jeopardy for experiencing negative educational and occupational outcomes, problematic substance use, criminal offending, and sexual victimization. Alcohol mixed with energy drink (AmED) use is widespread across college campuses and associated with many of these same problematic outcomes. The risk of experiencing these consequences for college students with ADHD may be exacerbated by AmED use given its unique relationship, above and beyond alcohol use, with these variables. This study sought to examine relationships among GPA, lost work performance, hazardous alcohol use, illicit substance use, criminal offending, and sexual …

Evaluating The Pennebaker Paradigm With Bereaved Emerging Adults: Applications Of Text Analysis, Elizabeth A. Collison

Theses and Dissertations

Bereavement is an important research area as it can result in grief reactions that lead to serious psychological and health consequences, particularly for the at-risk group of emerging adults (Arnett, 2000; Balk, Walker, & Baker, 2010; Fisher, Murray, & Frazer, 1985; Stroebe, Schut, & Stroebe, 2007). Expressive writing is a well-researched intervention for trauma and adjustment, yet research repeatedly has revealed null results with the classic Pennebaker paradigm as a bereavement intervention (Stroebe et al., 2002; Stroebe, Schut, & Stroebe, 2006). It may be premature, however, to conclude expressive writing is ineffective for the bereaved due to limitations in extant …

Markerless Lung Tumor Trajectory Estimation From Rotating Cone Beam Computed Tomography Projections, Shufei Chen

Theses and Dissertations

Respiration introduces large tumor motion in the thoracic region which influences treatment outcome for lung cancer patients. Tumor motion management techniques require characterization of temporal tumor motions because tumor motion varies patient to patient, day to day and cycle to cycle. This work develops a markerless algorithm to estimate 3 dimensional (3D) lung-tumor trajectories on free breathing cone beam computed tomography (CBCT) projections, which are 2 dimensional (2D) sequential images rotating about an axis and are used to reconstruct 3D CBCT images.

A gold standard tumor trajectory is required to guide the algorithm development and estimate the tumor detection accuracy …

Student Engagement In Undergraduate Social Work Education Among “At-Risk” Students, Ananda Newmark

Theses and Dissertations

College student engagement is an important factor that contributes to student success. This study is one of the first to explore student engagement in undergraduate social work education by examining engagement levels among at-risk social work students. In this study, two types of at-risk student groups were studied: First Generation College Students (FGCS) and transfer students. A cross sectional research design was used. Secondary analysis was performed on data gathered by the National Survey of Student Engagement (NSSE) from five accredited, Bachelor of Social Work (BSW) programs in one southeast state. A sample of 135 BSW seniors were included in …

Involvement Of Signal Peptidase I In Streptococcus Sanguinis Biofilm Formation, Jessica Aynapudi

Theses and Dissertations

Biofilm accounts for 65%-80% of microbial infections in humans. Considerable evidence links biofilm formation to oral disease and consequently systemic infections. Eradication of biofilm-associated infections is important. Streptococcus sanguinis, a Gram-positive bacterium, is one of the most abundant species in oral biofilm. It contributes to biofilm development in oral cavities and is one of the recognized causes of infective endocarditis. To study and identify biofilm genes in S. sanguinis, biofilm formation of 51 mutants was compared with the wild type SK36 strain using crystal violet (CV) staining in a microtiter plate. Confocal laser scanning microscopy (CLSM) and image analysis was …

A Correlational Analysis Of Secondary Data For Factors Influencing Graduation From Adult Drug Court, David N. Masri

Theses and Dissertations

The proliferation of drug courts throughout the world over the last two decades presents an opportunity and a challenge. The drug court approach involves a combination of treatment and judicial supervision which is a diversion from incarceration and/or ‘traditional’ criminal justice supervision. Despite widespread study of drug courts, there is much that researchers still do not know and there is still controversy as to how and why drug courts work. This research study is an examination of secondary data from an urban, mid-Atlantic drug court to attempt to correlate factors that contribute to success (as defined by graduation) in drug …

Allosteric Effects Of G-Protein Coupled Receptor Heteromerization: Relevance To Psychosis, Jason W. Younkin

Theses and Dissertations

G-protein coupled receptors (GPCRs) implicated in disease are the predominant pharmaceutical targets. Growing evidence suggests that GPCRs form homo- and heteromeric complexes, resulting in allosteric functional changes. Ligands targeting one receptor can alter the function of the other receptor or receptors. Knowledge of these functional changes will provide unique opportunities to treat diseases. We examined two GPCR heteromers implicated in psychosis: mGlu2R-5HT2AR and D2R-5HT2AR. Using whole-cell patch clamp, we studied HEK-293 cells stably transfected with mGlu2R and 5HT2AR. Maximal heteromer formation allows inverse agonists to increase the G-protein activity of the opposite receptor, while sub-maximal heteromer formation does not. However, …

Exploring Visual Prevention: Developing Infographics As Effective Cervical Cancer Prevention For African American Women, Candace P. Parrish

Theses and Dissertations

The purpose of this dissertation is to explore the creation of a cervical cancer health prevention infographic, geared toward African American women ages 30 to 65, to be potentially used to raise health literacy and influence positive behaviors towards practicing prevention measures—ultimately preventing unnecessary deaths. This dissertation also produced a cervical cancer health prevention infographic prototype for African American Women ages 30 to 65 to be further tested and implemented within future health communication campaigns.

Cervical Cancer is both preventable and treatable (if diagnosed during early stages); therefore the amount of individuals dying from the cancer should be minimal. Still, …

Plant Functional Trait And Hyperspectral Reflectance Responses To Comp B Exposure: Efficacy Of Plants As Landmine Detectors, Paul V. Manley Ii

Theses and Dissertations

At least 110 million landmines have been planted since the 1970s in about 70 nations, many of which remain in place today. Some risk of detection may be mitigated using currently available remote sensing techniques when vegetation is present. My study focused on using plants as phytosensors to detect buried explosives. I exposed three species representing different functional types (Cyperus esculentus (sedge), Ulmus alata (tree), Vitis labrusca (vine)) to 500 mg kg^-1 of Composition B (Comp B; 60/40 mixture of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and 2,4,6-trinitrotoluene (TNT)), a commonly used explosive mixture, and measured functional traits and reflectance over …