Digital Commons Network^™

Investigation Of Dynamic Biological Systems Using Direct Injection And Liquid Chromatography Mass Spectrometry, Adam Clayton Swensen

Theses and Dissertations

In biological systems, small changes can have significant impacts. It is, therefore, very important to be able to identify these changes in order to understand what is occurring in the organism. In many cases, this is not an easy task. Mass spectrometry has proven to be a very useful tool in elucidating biological changes even at a very small scale. Several different mass spectrometry based techniques have been developed to discover and investigate complex biological changes. Some of these techniques, such as proteomics, have been through years of development and have advanced to the point that anyone can complete complex …

The Effects Of L-Cysteine On Alzheimer's Disease Pathology In Apoe2, Apoe3, And Apoe4 Homozygous Mice, Stephen Gerard Cieslak

Theses and Dissertations

The APOE gene is of profound importance regarding the onset of Alzheimer's disease (AD). From the small physical differences among the protein products of the isoforms of this gene arises a profound difference in their physiologies. For example, the APOE2 isoform confers resistance to AD, the APOE3 isoform confers neutral susceptibility to AD, and the APOE4 isoform confers proneness to AD. L-cysteine is an amino acid that has several anti-AD properties, among which are its ability to sequester iron and form glutathione – a powerful antioxidant – and therefore may be a promising potential dietary supplement for ameliorating AD …

A Pes Study Of Factors Influencing Metal Partitioning In Aquatic Systems: “Design Of Experiment As, Cd, Co, Cr, Cu, Pb, Ni, And Zn”, Eid A. Alkhatib, John Rapaglia, Leon Theim

Chemistry & Physics Faculty Publications

Mobility and bioavailability of heavy metals are related to their partitioning amongst suspended sediment and water. A Particle Entrainment Simulator (PES) is used to simulate sediment resuspention in natural surface water systems. The simulations were carried out under various conditions of water/suspended solids conditions. Five factors, each at various levels, are tested collectively: the pH of water at two levels (4 and 8), shear stress on bottom sediment at three levels (0.1, 0.3, and 0.5 N/m2 ) salinity of water at two levels (0.01 and 14.0 ppt), organic matter in sediment at three levels (0.50, 1.93, and 3.80%) and temperature …

Spatial Distribution Of Antibiotic Resistance In Soils Receiving Beef Feedlot Runoff, Scott Speicher

Department of Environmental Engineering: Theses and Other Student Research

A study was conducted to provide new insight on the potential contribution to antibiotic resistance from the land application of beef feedlot runoff to soil. This study reports the distribution and quantity of antibiotic resistant bacteria (ARBs), fecal indicator bacteria (FIB), and antibiotic resistance genes (ARGs) in soil from (i) a field receiving long-term application of beef feedlot runoff holding pond effluent and (ii) a cool-season pasture with no history of supplemental manure application.

Soil samples were collected June 2015 at the U.S. Meat Animal Research Center near Clay Center, Nebraska. A response surface sampling design (RSSD) model based on …

Models For Decanting Gaseous Fuel Tanks: Simulations With Gfssp Thermal Model, Kailash Kumar Jain Munoth

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Transport of fuel from distillation/storage plant to different parts of the world has always been challenging task for Engineers. Different methods have been proposed over the time for transporting fuel efficiently and at low cost which include Marine vessels, Pipelines, Rail Cars and Trucks. In order to transport useful amount of fuel in a reasonably sized tank, we have to liquefy it. While few fuels are easy to liquefy there are great number of fuels which liquefy only under extreme pressure/temperature conditions. Methane has a boiling point of -161.7°C at atmospheric pressure which means it has to be cooled to …

Nuclear Factor [Erythroid-Derived 2]-Like 2’S (Nfe2l2) Gene Expression In Feed Efficiency And Oxidative Stress In Avian Muscle, Devin Wayne Cook

Graduate Theses and Dissertations

Oxidative stress may play a role in the phenotypic expression of feed efficiency (FE). The transcription factor NFE2L2 (nuclear factor erythroid-derived 2-like 2) coordinates antioxidant response to oxidative stress and its activity is tightly regulated in part by KEAP1 (Kelch like-ECH protein 1) and the E3 ligase CUL3 (Cullin3). Thus, one objective was to determine mRNA expression of NFE2L2, KEAP1, and CUL3 as well as three antioxidant targets [glutathione peroxidase (GPx-1), superoxide dismutase 1 (SOD1), and superoxide dismutase 2 (SOD2)] in breast muscle of immature pedigree broiler males (8 wk), immature Japanese quail males (4 wk) divergently selected for high …

The Bg News December 01, 2016, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. December 01, 2016. Volume 96-Issue 30.

Using Soil Conditioners To Improve Soil Physiochemical Properties And Agricultural Productivity In Libya, Gandura Abagandura

All Dissertations

The results of Libyan agricultural review confirmed that soil degradation and limited freshwater resources are the primary causes of the country’s dependence on food imports. The intrusion of the seawater into the groundwater is causing soils to become salt-affected. In addition, many crops in the country are susceptible to pests. The spatial distribution of soil degradation occurrence and type were predicted using regression models, estimating that 666 882 km2 (53.5 %) of Libyan soils are degraded with 46.4 % due to salinization, 6.4 % water erosion and 0.66 % wind erosion.

The majority of the soils in Libya are sandy …

Fluorescent Methods For Investigating Metabolic Processes In Trypanosoma Brucei, Sheng Lin

All Dissertations

Trypanosoma brucei is a unicellular kinetoplastid protozoan that is the causative agent of Human African Trypanosomiasis, or Sleeping Sickness. Combating these protozoan pathogens presents an ongoing challenge due to their efficient immune evasion strategies, lack of effective vaccines, poor drugs and emerging drug resistance, and the general lack of knowledge of their unique biological processes. Among one of the most important biological pathways for kinetoplastid protozoans is the synthesis of ATP from glucose within glycosome organelles. Glycosomes are related to mammalian peroxisomes, but differ in having a fully compartmentalized glycolysis pathway in addition to basic peroxisomal functions. While equivalent processes …

Me Of The Lowercase Variety: Reflections On The American Dream, Power, Privilege, And Self, Jared M. Halter

All Dissertations

This critical qualitative, autoethnographic research project explored how i came to understand the American dream through stories shared with me during semi-structured interviews, scholarly literature, and my own lived experience. i utilized critical reflexivity to examine the ways in which race, gender, and class appear in a person's understanding of their life and the American dream. Critical whiteness theory, critical race theory and critical race feminism, and structuralist constructivism guided my understanding of the American dream and how it influences the ways in which individuals and i understand our lives. Through what i learned, i discuss how the American dream …

Release And Degradation Of Microcystin-Lr Following Exposures Of Microcystis To Copper-Based Algaecides, Kyla Iwinski

All Dissertations

Microcystins (MCs) are hepatotoxic endotoxins produced by cyanobacteria such as Microcystis that can cause both ecological and human health risks, as well as economic losses in freshwater resources. The use of copper-based algaecides to manage MC-producing cyanobacteria is sometimes limited due to perceived risks of MC release from cyanobacterial cells and persistence of toxins in water resources. The aim of this research was to investigate the influence of copper concentration and form on MC release and persistence, as well as potential consequences of “no-action” management decisions (i.e. no treatment). To achieve these objectives, a tiered approach was employed utilizing both …

Where Cultures Collide: Hispanic Family Involvement In Education Among Different Socioeconomic Groups, Holly M. Grover

All Dissertations

This dissertation explores from a cultural perspective how socioeconomic status influences Hispanic family involvement in a student's education. Specifically, the study examined types of family involvement practices common to Hispanic families in high and low socioeconomic groups, how much these practices influence student outcomes, and the mechanisms through which involvement is related to student outcomes. Information for a nationally representative stratified probability sample of more than 1600 Hispanic students and their families was obtained from the Education Longitudinal Study of 2002, utilizing information from waves in 2002, 2004, and 2006. A Structural Equation Model evaluated the relationship between family involvement …

A Word From The Writing Center (December 2016), Jennifer Wilson, Ms, Els

A Word From the Writing Team (Newsletter)

This issue includes:

• Writing tip
• Books on writing
• Writing Café

Front Matter

The Foundation Review

No abstract provided.

Aiming High: Foundation Support For State Advocates Brings Universal Children’S Health Coverage Within Reach, Sheila Dunleavy Hoag, Debra J. Lipson, Victoria Peebles

The Foundation Review

To help close the children’s health insurance coverage gap in the United States, in 2011 the Atlantic Philanthropies created the Kids- Well Campaign. KidsWell’s theory of change posits that if advocates could leverage new funding and coverage opportunities created by the Patient Protection and Affordable Care Act, they could expand the number of children with health insurance coverage.

This article presents the major results of the KidsWell evaluation, which found substantial progress in achieving KidsWell interim policy changes and coverage outcomes. But advocates still have a full agenda, which means grantees and funders need to redouble efforts to educate the …

Massive Pulmonary Embolism Presenting As Seizures, Shahrukh Hashmani, Fateh Ali Tipoo Sultan, Murtaza Kazmi, Amammarah Yasmeen

Department of Pathology and Laboratory Medicine

Pulmonary embolism can present in various ways, though seizure activity has been rarely reported. We report a 38 years old male who had met a road traffic accident, underwent surgery, presented a month later with seizures, chest pain, shortness of breath and low blood pressure. Massive pulmonary embolism was diagnosed on CTPA, with classical features on ECG and echocardiogram and the patient was successfully managed with thrombolysis. Massive pulmonary embolism (MPE) should be kept in mind in patients who present with seizures, chest pain and haemodynamic instability.

Alternate Efflux Pump Mechanism May Contribute To Drug Resistance In Extensively Drug-Resistant Isolates Of Mycobacterium Tuberculosis, Akbar Kanji, Rumina Hasan, Ambreen Zaver, Asho Ali, Kehkashan Imtiaz, Mussarat Ashraf, Taane G. Clark, Ruth Mc Nerney, Samreen Shafiq, Zahra Hasan

Department of Pathology and Laboratory Medicine

No abstract provided.

Mycobacterial Contamination Of Bronchoscopes: Challenges And Possible Solutions In Low Resource Settings, Dania Khalid Saeed, Sadia Shakoor, Seema Irfan, Rumina Hasan

Department of Pathology and Laboratory Medicine

The use of bronchoscopes has increased in tuberculosis (TB) diagnostics to circumvent the diagnostic challenges that are associated with low sputum volume and smear-negative TB. In healthcare facilities situated in low income countries that have a high burden of TB, adequate decontamination of bronchoscopes is a challenge and often overlooked to save on time and costs. This amplifies the risk of outbreaks and pseudo-outbreaks due to Mycobacterium tuberculosis and nontuberculosis mycobacteria. In this minireview, we review published literature of contaminated bronchoscopes causing pseudo-outbreaks of M. tuberculosis and nontuberculosis mycobacteria in an effort to determine common sources, and possible mitigation strategies …

Pulmonary Infections After Tuberculosis, Kauser Jabeen

Department of Pathology and Laboratory Medicine

Aims and Objective: Despite effective treatment of pulmonary tuberculosis (TB) patients, destruction of lung parenchyma may lead to complications including repeated infections. These infections are often misdiagnosed or wrongly identified as TB recurrence, and hence are not treated effectively. The frequency and severity of these infections vary with the extent of damage, and are much more prominent in patients with post-TB bronchiectasis and fibrocavitary diseases. This presentation will focus on the epidemiology, treatment, and management of post-TB infections and challenges, and the impact of these infections on public health in high-TB-burden countries.
Methods: Published literature and review articles were evaluated …

Tuberculosis In Vulnerable Populations In Eastern Mediterranean Region-Implications For Control, Sadia Shakoor, Rumina Hasan

Department of Pathology and Laboratory Medicine

Objectives: Socially and economically disadvantaged or "vulnerable" people are at high risk of tuberculosis (TB) and also contribute to active chains of TB transmission. Included in such vulnerable populations are children, women, prisoners, people living with human immunodeficiency virus, the homeless, and displaced people. The ongoing active transmission of TB among such populations is made more difficult to assess and control by difficult access, health inequities, poverty, and other chronic and debilitating health conditions at individual, domestic, and community levels.
Methods: The 22 Eastern Mediterranean Region member states encompass diverse sociopolitical and socioeconomic situations with far-reaching effects on vulnerable populations …

Executive Summaries

The Foundation Review

No abstract provided.

Editorial, Teresa R. Behrens

The Foundation Review

No abstract provided.

Through The Looking Glass: Foundation Evaluation And Learning And The Quest For Strategic Learning, Suzanne Kennedy Leahy, Sandra Wegmann, Lexi Nolen

The Foundation Review

Strategic learning, a critical if relatively new lens for philanthropy, is neither simple nor efficient to institutionalize or practice yet — foundations are still figuring out how to do it well. In 2015, the Episcopal Health Foundation launched a project to distill lessons about how leading foundations configure evaluation and learning, and how they allocate related responsibilities in support of strategic learning.

This article addresses different models that foundations use to establish and staff evaluation and learning functions, what other organizational considerations they should take into account in order to prioritize strategic-learning work, and what tools and approaches can be …

Full Issue

The Foundation Review

No abstract provided.

Lessons About Evaluating Health-Coverage Advocacy Across Multiple Campaigns And Foundations, Leslie Foster, Mary Harrington, Sheila Hoag, Debra Lipson

The Foundation Review

This article draws on a dozen years’ experience in evaluating major consumerhealth advocacy initiatives to build the knowledge base about advocacy evaluation. The authors explain how their evaluations were strengthened by articulating a detailed theory of change and emphasizing assessment of interim outcomes from many perspectives and methods.

Even with comprehensive data and integrated analysis, however, some ambiguity in the results is inevitable; there is no completely objective way to determine the effectiveness of an advocacy initiative. Moreover, sometimes solid or even exceptional advocacy efforts do not lead to desired policy outcomes. Advocacy initiatives that fail initially may be groundwork …

Designing Technical-Assistance Programs: Considerations For Funders And Lessons Learned, Jennifer Lyons, Sheila Dunleavy Hoag, Cara Orfield, Sonya Streeter

The Foundation Review

As foundations continue to provide grantees with technical assistance in addition to financial support, it is important to understand what works well, for whom, and in what circumstances. This reflective practice article aims to help funders who have identified a problem amenable to technical assistance to develop a strong program by providing support to a group of organizations addressing similar problems or by providing customized individual support.

Drawing on insights from evaluations of two technical-assistance programs, this article recommends five key issues for funders to consider when offering such a program: whose priorities will shape the agenda, how group composition …

Back Matter

The Foundation Review

No abstract provided.

Pesticide Resistance In Plutella Xylostella (Lepidoptera: Plutellidae) Populations From Togo And Benin, L. K. Agboyi, G. K. Ketoh, T. Martin, I. A. Glitho, M. Tamò

All Peer-Reviewed Publications

The diamondback moth, Plutella xylostella (L.) is the major insect pest of cabbage crops in Togo and Benin. For control, farmers very often resort to spraying chemical insecticides at high dosages with frequent applications. Bioassays were carried out on three populations of P. xylostella, two from Togo (Kara and Dapaong) and one from Benin (Cotonou), to assess their level of susceptibility to currently used insecticides. A reference strain of P. xylostella from Matuu in Kenya was used as a control. In the laboratory, three insecticide representatives of different chemical families (deltamethrin, chlorpyrifos ethyl and spinosad) were assayed against third instar …

Managing Storage Pests Of Maize: Farmers' Knowledge, Perceptions And Practices In Western Kenya, Charles A.O. Midega, Alice W. Murage, Jimmy O. Pittchar, Zeyaur R. Khan

All Peer-Reviewed Publications

Insect pests are a key constraint to effective utilization of cereal crops in sub-Saharan Africa (SSA), with damage caused by these pests in the stores of particular concern. Although a number of approaches have been advanced for control of storage pests of maize, uptake remains a challenge, with effectiveness of some approaches being questionable. We conducted a survey in western Kenya among 330 respondents using face to face interviews and focus group discussions to evaluate farmers' practices, knowledge and perceptions of storage pests of maize, and their current practices in managing such pests as a basis for development of efficient …

Agricultural Adaptation To Drought In The Sri Lankan Dry Zone, Emily Burchfield, Jonathan Gilligan

Environment and Society Faculty Publications

Droughts affect more people than any other natural disaster. Drought severity is not merely a function of precipitation; it emerges from a web of interrelations between human and natural systems. The impacts of drought are equally complex, shifting across temporal scales, economic sectors, and regions. Even in regions with similar hydroclimatic characteristics, there is tremendous variation in the effects of drought. This study combines satellite imagery, geospatial data, and qualitative data to identify the multi-scalar factors that drive variations in agricultural responses to drought. We analyzed eleven years of remotely sensed imagery to identify agricultural areas in which cultivation occurred …