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Lactose-Rich Milk Replacer Modifies The Proteome Of Bloodplasma In 2-Week-Old Calves, Alicja Dratwa Chalupnik, Malgorzata Ozgo, Agnieszka Herosimczyk, Adam Lepczynski, Katarzyna Michalek

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to create a specific pattern of protein expression changes in the plasma of 2-week-old calves treated with excessive lactose doses. Identification of proteins, the expression of which changes during the short-term administration of lactose, may help to exclude or confirm the causes of diarrhea observed in newborn calves. The present study utilized 2-D electrophoresis combined with MALDI-TOF mass spectrometry and identified 6 blood plasma proteins, the expression of which changed under the influence of lactose added to the milk replacer. The pattern of expression of blood plasma proteins, including decreased expression of fibrinogen and …

Comparison Of Hematological Parameters Of Brucella-Seronegative Naturallyinfected Cattle With Bvdv, Bhv-1, And Bhv-4, Muhammet Eren Aslan, Ahmet Kürşat Azkur, Emel Biyikli, Serkal Gazyağci, İbrahi̇m Sözdutmaz

Turkish Journal of Veterinary & Animal Sciences

Abortion, neonatal deaths, and infertility cause important production losses in the livestock industry. Many viral infections are responsible for changes in hematological values of animals. This study aimed to find whether there is any difference in blood parameters of cattle that are naturally infected with BVDV, BHV-1, and BHV-4 and Brucella-seronegative. For this purpose, 401 whole-blood samples collected from cattle were analyzed by ELISA and PCR/RT-PCR to detect specific antibodies and antigens for BVDV, BHV-1, and BHV-4. Hematological analysis and results were compared between naturally infected BVDV, BHV-1, and BHV-4 groups and a control group. Results showed a statistically significant …

Postnatal Expression Profiles Of Phospholipid Hydroperoxide Glutathioneperoxidase In Spermatogenesis In Mice, Ki Youn Jung, Chunmei Lin, Jung-Min Yon, A Young Jung, Seul Gi Park, Sl Bi Chu, Lee Wha Gwon, Sang Yoon Nam

Turkish Journal of Veterinary & Animal Sciences

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is known to be an antioxidant enzyme that may be implicated in spermatogenesis, but its functional significance in the development of testes remains poorly understood. To demonstrate the roles of PHGPx in spermatogenesis during postnatal development, expression profiles of PHGPx mRNA and protein in testes and epididymides obtained from mice 1-9 and 27 weeks after birth were spatiotemporally analyzed. In testes, PHGPx mRNA first appeared in 2-week-old mice, while its protein was first detected in late spermatocytes and round spermatids from 3-week-old mice and levels were greatly increased in elongated spermatids and immature spermatozoa of …

Subarachnoid Transplantation Of Autologous Neurogenically Induced Bone Marrow Derived Mesenchymal Stem Cells For The Treatment Of Fibrocartilaginous Embolic Myelopathy In Two Dogs, Pinar Can, Ömer Beşalti, Eylül Akpinar, Zeynep Aktaş, Ayşe Eser Elçi̇n, Yaşar Murat Elçi̇n

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the effects of autologous neurogenically induced bone marrow-derived mesenchymal stem cells (NIBM-MSCs) in two dogs suspected to have fibrocartilaginous embolic myelopathy (FCEM). Both dogs were paraplegic without deep pain perception (DPP) and tentatively diagnosed by MRI. Autologous NIBM-MSCs (5 × 106) were transplanted into the lumbar subarachnoid space two times with a 21-day interval for each patient. Based on a 21-month follow-up of the treated animals, the transplantation of NIBM-MSCs seems to be promising in subjects with FCEM lacking DPP.

Canine Brucellosis In Turkey From An Environmental Perspective, Uçkun Sai̇t Uçan

Turkish Journal of Veterinary & Animal Sciences

Brucellosis is a zoonotic disease that affects not only livestock with economical losses but also human beings by causing serious health problems worldwide. Canine brucellosis has largely been overlooked in the world. In Turkey, brucellosis has also been one of the major concerns for decades. Canine brucellosis in this country has been reviewed and discussed from an environmental perspective in this review. As a part of prevention measures, one of the urgent practices that should be applied is to expand the routine examinations to include serological testing for all animals on arrival to kennels and then to decide the fate …

Isolation Of Adipose Tissue-Derived Stem Cells, Asuman Özen, İrem Gül Sancak, Ahmet Ceylan, Özge Özgenç

Turkish Journal of Veterinary & Animal Sciences

Mesenchymal stem cells are being used increasingly in cell-based therapies. Adipose tissue is an important source of mesenchymal stem cells and is also routinely used in research on lipid metabolism and obesity. Their high expansion potential and the ease of isolation make these cells an attractive cell source for regenerative therapies. The objective of this review is to give detailed information about the isolation, expansion, and clinical use of these cells in veterinary medicine.

Effect Of Oxytocin On Milk Proteins And Fatty Acid Profile Insahiwal Cows During Lactation Periods, Aneela Hameed, Faqir Muhammad Anjum, Tahir Zahoor, Zia Ur-Rahman, Saeed Akhtar, Majid Hussain

Turkish Journal of Veterinary & Animal Sciences

Bovine milk, a rich source of proteins and fatty acids, has a beneficial impact on physicochemical and organoleptic properties. The current study was planned to identify oxytocin's effects on protein and fatty acid profiles of cow milk (the Sahiwal breed) at various lactation stages (early, middle, and late) under a controlled atmosphere and feeding inputs. Examination of milk protein and fatty acid profiles by urea polyacrylamide gel electrophoresis and gas chromatography-mass spectrometry respectively revealed the significant effect (P < 0.01) of oxytocin during the cow's lactation stages. In electrophoretic patterns, casein (αs1-CN, αs2-CN, β-CN, and k-CN) and whey protein fractions (Ig, BSA, β-Lg, and α-La) appeared as low-intensity bands in oxytocin-injected milk when compared to the control. Reductions in concentrations of fatty acids C16:0 (25.00%) and C18:1 (21.60%) were observed in oxytocin-treated milk as compared to the control group (29.30% and 29.10%, respectively) at late lactation.

Investigation Of Relationship Between Expression Of The Glucose Transporter3 (Glut3) And Sperm Quality In Asian Elephants (Elephas Maximus), Somchai Sajapitak, Kornchai Kornkaewrat, Piyawan Suthunmapinanta, Orawan Boodde, Sittidet Mahasawangkul, Anuchai Pinyopummin

Turkish Journal of Veterinary & Animal Sciences

The aims of this study were to determine the relation of glucose transporter 3 (GLUT3) expression on the spermatozoa membrane with the quality of semen in Asian elephants. Fresh semen samples were collected from 10 Asian elephants and classified according to the percentage of motile sperm into Group 1 (≤ 20%; n = 4 males), Group 2 (>20% to 60%; n = 3 males), and Group 3 (>60%; n = 3 males). The immunocytochemistry results showed the presence of GLUT3 at the principal and end piece of the sperm tail. GLUT3 expressions in each group were significantly different …

Effects Of Ultrasonication On Damaged Spermatozoa And Mitochondrial Activity Rate, Pinar Peker Akalin, Nuri̇ Başpinar, Kenan Çoyan, Mustafa Numan Bucak, Şükrü Güngör, Caner Öztürk

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to describe an optimal sonication procedure for sperm cells. Therefore, we used several parameters such as damaged spermatozoa rate (%), mitochondrial activity rate (%), levels of lipid peroxidation, and total antioxidant potential. Ejaculates were collected from rams (n = 3) and were divided into aliquots and 3-, 6-, and 10-s duration times; 1, 3, 5, and 8 repetitive application groups were established. In the groups with 3-, 6- and 10-s duration times, with the increasing number of repeated applications, damaged spermatozoa rates increased (P < 0.05) while mitochondrial activity rates decreased (P < 0.05). In relation with sonication duration time, total antioxidant potential levels increased (P < 0.05) in single-application groups compared to those in control groups and gradually decreased as the repetitions increased. The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate.

Characterization Of Porcine Adiponectin Gene (Adipoq) Polymorphismsand Their Association With Litter Size, Xiaodong Zhang, Ning Zhou, Jie Zhou, Ning Wang, Xiang Qin, Hui Dong, Yueyun Ding, Zongjun Yin

Turkish Journal of Veterinary & Animal Sciences

Litter size is one of the most important economic traits for pig production as it is directly related to production efficiency. To evaluate the effects of the adiponectin (ADIPOQ) gene on porcine litter size, we investigated ADIPOQ polymorphisms in three breeds, Wannan Black pigs, Berkshire pigs, and BW pigs (crossbred pigs produced from mating male Berkshire pigs and female Wannan Black pigs), using the PCR-SSCP method. Three SNPs (c. 178G > A, c. 1165A > G, and c. 1138G > A) were identified. In Wannan Black pigs, association analysis indicated that the c. 178G > A SNP genotype was significantly associated with an effect …

The Treatment Of Distal Metacarpus Fracture With Locking Compression Plate In Calves*, Ali̇ Belge, İbrahi̇m Akin, Ali̇ Gülaydin, Muhammed Fati̇h Yazici

Turkish Journal of Veterinary & Animal Sciences

In this study, we aimed to evaluate the effect of locking compression plates for the treatment of distal metacarpal fractures in calves. The metacarpus was stabilized using a stainless steel locking compression plate with fully threaded locking head screws. The calves started weight-bearing partially after the first postoperative day and resumed complete weight-bearing after day 10. Callus formation was obtained at the end of the 3rd week and completed at the end of the 8th week, and complete healing was seen at the 12th week in radiographs. The limbs were in good alignment, the calves were fully weight-bearing, and client …

The Effect Of Dgat1 Polymorphism On Milk Production Traits In Dairycows Depending On Environmental Temperature, Jolanta Komisarek, Mateusz Kolenda

Turkish Journal of Veterinary & Animal Sciences

It has long been known that a hot environment negatively influences milk production in cattle. Although molecular mechanisms underlying this phenomenon to a great extent remain to be recognized, they include alterations in lipid metabolism. DGAT1 plays a key role in the synthesis of triacylglycerols and polymorphism of this gene has been proven to have a pronounced influence on milk production. The aim of this study was to establish if the effect of DGAT1:p.K232A on milk-related traits is dependent on environmental thermal conditions. The analysis, performed on 468 cows of the Polish Holstein-Friesian breed, showed that allele DGAT1:K232 significantly increased …

Cryopreservation Of Seabream (Sparus Aurata) Semen And Fertility, Mehmet Borga Tirpan, Erkut Dervi̇ş Özgöray, Havva Alemdar, Ergün Akçay

Turkish Journal of Veterinary & Animal Sciences

In this study, the aim was to reveal spermatological parameters of seabream (Sparus aurata), freezing ability of semen in different extenders, and fertilization capacity of frozen seabream semen. The study was conducted with 10 male and 6 female brooder seabreams. Female fish were synchronized by photoperiod and water temperature in the breeding season. Four different extenders [Mounib, Salty Mounib, Ringer, and sea water (1% NaCl)] including 10% dimethyl sulfoxide were used to assess the freezing ability of seabream semen. After postthaw spermatological examinations, three successful extenders (Mounib, Ringer, 1% NaCl) were chosen for the fertilization stage and 2 control and …

Dimensional Characteristics Of Spermatozoa Of Nili-Ravi Buffalo Bulls: Seasonaland Climatic Influences, Sajid Hameed, Saima Masood, Hafsa Zaneb, Muhammad Sarwar Khan, Muhammad Younis, Muhammad Avais, Muti Ur Rehman Khan

Turkish Journal of Veterinary & Animal Sciences

This study was conducted in 2 different climatic zones of Punjab Province of Pakistan, the Sahiwal and Bahawalpur divisions, simultaneously for the period of 1 year divided into seasons. Seven adult Nili-Ravi buffalo breeding bulls from each division were used. Dimensional characteristics of spermatozoa were determined. Season had a nonsignificant effect on the sperm head length in both divisions and a nonsignificant difference was also observed between bulls of the Sahiwal and Bahawalpur divisions. The buffalo bulls of both divisions exhibited significantly increased sperm head breadth in autumn, whereas a nonsignificant difference was observed between divisions. Sperm head shape values …

Presence Of Carbohydrate-Digesting Enzymes Throughout The Digestive Tract Of Sheep, Renata Miltko, Grzegorz Belzecki, Barbara Kowalik, Jacek Skomial

Turkish Journal of Veterinary & Animal Sciences

The activity of enzymes that digest carbohydrates at various points in the gastrointestinal tract of sheep was examined. In addition, weight and contents of digestive tract segments were examined. The experiment was performed on six adult ewes fed an 85% hay diet. The morphometric measurements revealed that the alimentary tract constituted 32% of the sheep's body mass and wet rumen contents constituted 70% of wet mass of the total digestive tract content. The protozoal population in the rumen varied from 37.2 × 104 to 54.0 × 104/mL rumen fluid. In the examined samples, ciliates were identified as belonging to the …

Naegleria Fowleri Meningoencephalitis Associated With Public Water Supply, Pakistan, 2014, Najia Ghanchi, Erum Khan, Azam Khan, Wali Muhammad, Faisal Riaz Malik, Afia Zafar

Department of Pathology and Laboratory Medicine

No abstract provided.

Towards Trialectic Space: An Experiment In Cultural Misunderstanding And Disorientation, Clive Barstow

Research outputs 2014 to 2021

This paper presents a praxical model of collaboration between artists in Australia and China in which the misrepresentation and misinterpretation of language (Ang 1997) within Homi Bhabha’s (1990) thirdspace theories are examined. To avoid the polarities of othering (Dervin, 2014), I adopt Henri Lefebvre’s model of cumulative trialectics (1991) as a new thirdspace that more accurately represents the complexities of modern day geographies and hybrid communities by extending the binary analysis of the past and present and beyond the real and the imagined. Trialectics expand our understanding beyond physical geographies by suggesting a cerebral space that searches for new meaning …

Everything Old Is New Again: Enforcing Tribal Treaty Provisions To Protect Climate Change-Threatened Resources, Elizabeth Ann Kronk Warner

Nebraska Law Review

In an age when vulnerable tribes and Native communities around the country are threatened by the impacts of climate change, advocates seek new and innovative legal tools to provide protection for endangered resources. To date, legal tools such as litigation and adaptation plans have been used with varying levels of success. For the first time, this article considers whether tribal treaties with the United States may prove helpful in protecting threatened resources. Treaties historically played an important role for many tribes, as they have a profound cultural connection and are a powerful expression of tribal sovereignty. Also, when courts find …

Al Maqaleh And The Diminishing Reach Of Habeas Corpus, Rehan Abeyratne

Nebraska Law Review

I. Introduction

II. Al Maqaleh: Background and Procedural History … A. Facts of the Case ... B. The Four Major Opinions ... 1. Al Maqaleh I ... 2. Al Maqaleh II ... 3. Al Maqaleh III ... 4. Al Maqaleh IV

III. Revisiting the Site of Apprehension and Site of Detention Factors ... A. Site of Apprehension ... B. Site of Detention ... 1. Is Sovereignty a Necessary Condition for Writ Jurisdiction? ... a. De Facto Sovereignty ... b. Eistentrager’s Holding ... 2. Conflating Degree and Duration of Control ... 3. A “Permanent” U.S. Presence at Bagram

IV. When …

The Animal Welfare Act At Fifty: Problems And Possibilities In Animal Testing Regulation, Courtney G. Lee

Nebraska Law Review

I. Introduction

II. Background of the Animal Welfare Act ... A. Enactment and Evolution ... B. Early Amendments ... C. Improved Standards for Laboratory Animals Act of 1985 ... D. Institutional Animal Care and Use Committees ... E. IACUC Effectiveness

III. Coverage of the AWA ... A. What Is an “Animal” under the AWA? ... B. Legislative Background of the Definition ... C. Sentience of Unprotected Species

IV. The Effectiveness and Necessity of Animal Testing ... A. Is Animal Experimentation Effective? ... B. Alternatives to Animal Testing ... 1. In Vitro Testing ... 2. In Silico Testing ... 3. Alternative …

Masthead & Table Of Contents, Vol. 95, No. 1

Nebraska Law Review

No abstract provided.

Grainyhead-Like 2 Reverses The Metabolic Changes Induced By The Oncogenic Epithelialmesenchymal Transition: Effects On Anoikis, Joshua C. Farris

Graduate Theses, Dissertations, and Problem Reports (ETD)

When deprived of connections to their extracellular matrix, normal epithelial cells trigger a process of programmed cell death referred to as anoikis. The transcriptional reprogramming event known as Epithelial-Mesenchymal transition (EMT) confers anoikis resistance and ultimately, increased metastatic potential. Distant metastatic disease contributes to the majority of deaths occurring in many cancer types, especially those originating in breast tissue. The ability to metastasize depends critically upon a cancer cell's ability to evade anoikis. Heterogeneity of tumors containing anoikis, therapy resistant populations likely explains the tendency of disease to reappear in distant locations after a period of apparent remission. The wound …

Nutritional Consequences Of Various Ingredients In Broilers, Turkeys, And Swine, Kolby L. Foltz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Experiments were conducted to evaluate various ingredients' effects on broiler, turkey, and porcine performance, as well as feed mill efficacy. In chapter 2, the effects of zinc (Zn) supplement source and corn particle size on broiler performance, breast yield, and tibia ash were assessed from d 1-40. Zinc treatments included a basal diet (no added Zn), 80 mg/kg Zn sulfate, and three diets with 40 mg/kg Zn sulfate + 40 mg/kg of varying Zn amino acid chelates, and corn particle size was either 550 mum or 1,050 mum. Broilers fed diets containing 550 ?m corn had higher feed intake (FI) …

Molecular Characterization Of Lhx8, An Oocyte-Specific Transcription Factor, And Its Interacting Proteins In Rainbow Trout And Cattle, Liyuan Fu

Graduate Theses, Dissertations, and Problem Reports (ETD)

LIM homeobox 8 (Lhx8) is an important transcription factor that is preferentially expressed in germ cells. Lhx8 null mice are infertile due to lack of oocytes and impairment of the transition from primordial follicles to primary follicles. Lhx8 deficiency also affects the expression of many important oocyte-specific genes. To date, no attempts have been made to investigate the existence of any cellular factors that might interact with Lhx8 protein in oocytes and early embryos. In this study, we report the characterization of rainbow trout and bovine Lhx8 genes and identification of important germ cell-specific nuclear factors that interact with Lhx8 …

Homeplace, Megan Leigh Gainer

Graduate Theses, Dissertations, and Problem Reports (ETD)

The sculptural work presented in this thesis and the accompanying exhibition showcase the ideas of memory, its fallacy, and why certain objects become tied to the idea of a specific time, place, and culture. Using my own personal experiences growing up in rural West Virginia, I call upon traditional crafting techniques to relay a sense of nostalgia commonly found throughout Appalachia. The expression of the hand present throughout the work highlights the importance placed on these seemingly mundane objects, which hold a special place in my history.

A Cbct Evaluation Of Root Position Within Bone, Long Axis Inclination, And The Wala Ridge, Timothy R. Glass

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objectives: Correct tooth position in all planes of space while respecting the boundaries of the underlying bone has been proposed as a necessary hallmark to providing a foundation of stability for the teeth as well as the supporting periodontium. The aim of this study was to determine 1) If teeth centeredness over basal bone improves when teeth are more upright or approach WALA Ridge norms 2) If teeth centeredness in alveolar bone improves when teeth are more upright or approach WALA Ridge norms 3) If the WALA ridge is located at or near the estimated center of resistance …

Role For Protein Prenylation And "Caax" Processing In Photoreceptor Neurons, Nachiket D. Pendse

Graduate Theses, Dissertations, and Problem Reports (ETD)

The efficient folding, assembly and trafficking of phototransduction proteins from site of synthesis in the inner segment (IS) to the site of action in the outer segment (OS) is crucial for photoreceptor cell survival and function. Defects in this process are known to cause blinding diseases in humans including retinitis pigmentosa (RP) and Leber's congenital amaurosis (LCA). The lack of a therapeutic approach for treatment of various retinal degenerations is likely due a knowledge gap in the mechanisms underlying the folding, assembly and transport of prenylated phototransduction proteins. Prenylation and " CAAX" processing is thought to be involved not only …

Grainyhead-Like-2 Inhibits The Co-Activator P300, Suppressing Tubulogenesis And The Epithelial-Mesenchymal Transition, Phillip M. Pifer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tumor progression and developmental morphogenesis require transient or stable Epithelial-Mesenchymal transitions (EMT) to modulate epithelial junctional complexes allowing programmed migration, invasion, and anoikis resistance of cells. Grainyhead-like-2 (GRHL2), an epithelial master-regulatory transcription factor, suppresses EMT and causes a mesenchymal-epithelial transition to the default epithelial phenotype. This body of work investigates the role of GRHL2 in kidney cancer, in kidney development using a kidney specific GRHL2 knockout mouse model, and in tubulogenesis using Madin-Darby Canine Kidney (MDCK) cells. In kidney cancer, GRHL2 was not expressed in the normal kidney proximal tubule epithelial cells which are thought to be the progenitors of …

The Role Of Art Education In The K-12 Curriculum In West Virginia A Comparative Case Study Using Ethnographic Methods, Helen G. Poffenbarger-Jordan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The visual arts are considered a core subject in the United States public school curriculum. However, the way in which visual arts curriculum is implemented in schools is largely left up to the individual states. This study focused on how art educators' design, direct, find support, and operate visual arts programs in the K-12 public school system in West Virginia. Ethnographic research methods were implemented for this study.

Design And Evaluation Of Functional Gear For Protection, Fit And Mobility, Ruchireeka Rath

Graduate Theses, Dissertations, and Problem Reports (ETD)

Functional wearables are a growing field at the intersection of fashion and technology. This research centers on the conceptual development of a fastening system in a functional wearable - fishing footwear - by assimilating utilitarian design values in a product design model. A project-based research methodology utilizing an iterative design process was used to create a multifunctional and technologically enabled closure system in fishing footwear. As part of the planning phase, an extensive review was conducted. This exploration substantiated the need for research centered around fastening systems for fishing footwear. A thorough investigative study was conducted as part of research …