Digital Commons Network^™

The Politics And Ethics Of Food Localism: An Exploratory Quantitative Inquiry, Sean T. Doody

Theses and Dissertations

The local food movement has become a prominent force in the U.S. food market, as represented by the explosive expansion of direct-to-consumer (DTC) marketplaces across the country. Concurrent with the expansion of these DTC marketplaces has been the development of the social ideal of localism: a political and ethical paradigm that valorizes artisanal production and smallness, vilifies globalization, and seeks to recapture a sense of place and community that has been lost under the alienating conditions of capitalism’s gigantism. Supporters of localism understand the movement to be a substantial political and economic threat to global capitalism, and ascribe distinct, counter-hegemonic …

Determining The Effect Of Knocking Out Microrna-21 On Subsarcolemmal And Interfibrillar Mitochondria, Madhur Batra

Theses and Dissertations

Type 2 diabetes mellitus is a growing problem across the world and has significant pathological changes associated with it, including diabetic cardiomyopathy, wherein cardiac function is reduced. MicroRNA-21 has been shown to play a role in both the heart and diabetes so it was thought that knocking out miR-21 could have a protective effect on oxidative phosphorylation function in diabetic mice. Subsarcolemmal and interfibrillar mitochondria were isolated from adult male WT, miR-21 KO, db/db, and double knockout mice (db/db and miR-21 KO cross) and evaluated for function. Knocking out miR-21 in diabetic mice showed a restorative effect in Complex I …

Identification Of Size And Shape Changes In Orofacial Development And Disease, Allyson E. Kennedy

Theses and Dissertations

Among the most prevalent and devastating types of human birth defects are those affecting the mouth and face, such as orofacial clefts. Children with malformed orofacial structures undergo multiple surgeries throughout their lifetime and struggle with facial disfigurements, speech, hearing, and eating problems. Therefore, facilitating new research in cranio- and orofacial development is paramount to prevention and treatment of these types of birth defects in humans. Xenopus laevis has emerged as a new tool for dissecting the mechanisms governing facial development. Thus, molecular analyses accompanied by quantitative assessment of morphological changes during orofacial development of this species could be very …

The Microbiome In Light Of Host Evolution, Alexander M. Waldrop Jr.

Theses and Dissertations

Recent advances in sequencing technologies have provided an unprecedented window in the unseen biological world. Accompanying this revolution is a growing appreciation for the ubiquity and diversity of beneficial interactions between animals and the microbes they carry. Given the symbiotic roles of microbes in host nutrition, immunity, behavior, development, and nearly every other facet of host biology, it is becoming increasingly clear that any understanding of hosts and their evolution would be incomplete without also considering the microbial dimension. Yet despite the growing body of evidence that many of these partnerships are rooted deep in evolutionary time, the majority of …

Genome Wide Epigenetic Analyses Of Araptus Attenuatus, A Bark Beetle, Chitra Seshadri

Theses and Dissertations

Phylogeographic studies have relied on surveying neutral genetic variation in natural populations as a way of gaining better insights into the evolutionary processes shaping present day population demography. Recent emphasis on understanding putative adaptive variation have brought to light the role of epigenetic variation in influencing phenotypes and the mechanisms underlying local adaptation. While much is known about how methylation acts at specific loci to influence known phenotypes, there is little information on the spatial genetic structure of genome-wide patterns of methylation and the extent to which it can extend our understanding of both neutral and putatively adaptive processes. This …

A Neural Circuit Of Appetite Control In C. Elegans, Kristen C. Davis

Theses and Dissertations

Feeding behavior and its associated neural circuitry is complex and intricate in mammalian systems, however, a simple model organism, such as C. elegans provides a more basic approach to understand factors and molecules involved. The fruit-dwelling nematode provides a unique set of resources; it only consists of 959 cells, 302 of which are neurons. In addition, each neuron’s connectivity and position within the worm is known and consistent between animals. Conservation of neurotransmitters and biochemical processes add to this impressive list. These resources provide an excellent background to address feeding behavior and the neural structures governing it.

Feeding behavior in …

Role Of Free Fatty Acid Receptors In The Regulation Of Brain-Derived Neurotrophic Factor Levels In The Enteric Glial And Endocrine Cells, Reem M. Alkahtani

Theses and Dissertations

Recent deorphanization of several G-protein-coupled receptors (GPRs) as endogenous receptors for free fatty acids (FFAs) has increased our understanding of these nutrients as signaling molecules and the integral role of gut microbiota in the regulation of host defense mechanisms, energy metabolism, gastrointestinal motility and secretion. There are five distinct FFA receptors (FFRAs: FFAR1, FFAR2, FFAR3, FFAR4 and GPR84) that differ in molecular structure, ligand specificity, expression pattern, and functional properties. FFARs are grouped according to the chain length of FFAs that activate each FFAR.

Enteric glial cells and enteroendocrine cells play a pivotal role in the secretion of gliotransmitters and …

Ancient Puebloan Human Effigy Vessels: An Examination Of Iconography And Tradition, Amy G. Marshman

Theses and Dissertations

This dissertation provides an iconographic interpretation of a group of Ancient Puebloan human effigy vessels and fragments from the American Southwest, dating to the Pueblo II period, c. 900 -1150 CE. Initially, this project focuses on Ancient Puebloan human effigy vessels from three specific collections; a single vessel in the collection of the National Museum of the American Indian in Washington, D.C., a human effigy vessel in the collection of the American Museum of Natural History in New York, and the so-called Putnam Human Effigy Jar from Chaco Canyon at the Peabody Museum of Archaeology and Ethnology at Harvard University. …

Differential Reactivity Of Microglia In Two Mouse Models Of Multiple Sclerosis, Rebecca K. Hartley

Theses and Dissertations

Multiple sclerosis (MS) is a neurodegenerative disorder characterized by CNS inflammation and axonal demyelination. In addition, axonal pathology has also been reported in MS and may be responsible for the functional deficits associated with this disease. Based on preliminary data from our laboratory, we propose that a specific domain of the neuron, known as the axon initial segment (AIS), is targeted in MS. Consistent with our work from the human tissue, we have also observed disruption of AIS integrity in a murine CNS inflammatory model and observations strongly implicate reactive microglia as mediators of AIS disruption. In contrast, a murine …

Effects Of Traumatic Brain Injury On Oxycodone Reinstatement And Physical Dependence, Neil B. Varshneya

Theses and Dissertations

Epidemiological data indicate that patients who experience a traumatic brain injury (TBI) have an elevated risk of developing a substance use disorder (SUD), but the underlying neurobiological connections remain unclear. To further understand the relationship between TBI and SUD, we investigated the effects of TBI on the abuse-related effects of oxycodone in preclinical models. Our evaluation utilized a lateral fluid percussion injury of moderate severity in adult male Sprague-Dawley rats. In the first aim, we tested the hypothesis that moderate TBI increases the risk for relapse to an opioid use disorder as measured by reinstatement of lever-pressing behavior following extinction …

Resolution Of Inflammation Rescues Axon Initial Segment Disruption, Nicholas M. George

Theses and Dissertations

Axonal domains are required for proper neuron function. These domains are unstable and degenerate concurrent with the inflammation in multiple sclerosis (MS) and the inflammatory disease models experimental autoimmune encephalomyelitis (EAE) and lipopolysaccharide (LPS) induced inflammation. Previous studies from our laboratory have shown that the axon initial segment (AIS) is maintained independently of the presence of myelin, but that AIS disruption is seen in MS as well as EAE and LPS-mediated inflammation. AIS loss can be interrupted in the early stage of EAE using the anti-inflammatory drug Didox. However, the potential for Didox directed repair of the AIS in later …

Behavioral Phenotyping Of Vmat1 Knockout Mice: Relevance To Neuropsychiatric Disorders, Kevin A. Webster Ph.D.

Theses and Dissertations

Schizophrenia is a debilitating mental disorder that causes a large economic burden and is prevalent across all cultures and countries around the world. Although both environmental factors and genetics are known to play an important role in the etiology of schizophrenia, the exact role of genetics and its interaction with environmental factors in an individual’s predisposition to develop schizophrenia is poorly understood. Schizophrenia is characterized by symptoms that include positive symptoms (e.g. delusions, hallucinations, disorganized thinking and speech), negative symptoms (e.g. avolition, anhedonia, depressive-like behavior), and cognitive dysfunctions (e.g. executive functioning deficits in learning and memory, attention, and vigilance). Genomic …

Hidden In Plain Sight: Image, Text, And Social Commentary In Victor Ekpuk's Cartoons For The Daily Times Of Nigeria, 1989-1998, Kaleb W. Jewell

Theses and Dissertations

This thesis provides an analysis of the cartoons produced by Victor Ekpuk for The Daily Times newspaper of Nigeria from 1989 through 1998 and the artist’s use of ancient nsibidi script to “hide in plain sight” his social commentaries on sociopolitical and economic issues in Nigeria. Victor Ekpuk’s original cartoons within the permanent collection of the Smithsonian Institution’s National Museum of African Art are examined in the context of indigenous masking practices and other indirect methods employed by indigenous comedians to protect themselves. Moreover, the cartoons’ use of caricature and their nsibidi scripts within are argued to provide a connotative …

Influence Of Historic Landscapes And Contemporary Species Management On Chesapeake Bay Bald Eagles And Osprey, Catherine B. Viverette

Theses and Dissertations

Influence of historic landscapes and contemporary species management on Chesapeake Bay Bald Eagles and Osprey

Catherine B. Viverette

Co-distributed species with well documented demographic histories can provide good models for testing alternative hypotheses about the impact of evolutionary history, contemporary landscapes, and species management on current distribution and population structure. The Osprey (Pandion haliaetus) and Bald Eagle (Haliaeetus leucocephalus) have been extensively studied, managed and monitored across their North American breeding range, particularly in the Chesapeake Bay. We used a combination of ecological niche modelling, diet reconstruction, and population genetic modeling to understand the role of …

Allegories Of The Modern: The Female Nude In Art Nouveau, Emily Winthrop

Theses and Dissertations

Modernism is a plurality, not a singular concept. This project explores examples of Art Nouveau nudes to describe the particular expressions of the modern through varied and complicated allegorical bodies. The female nude as a nexus for ideals of gender, art, and beauty, is informed by and constructs the understanding of these ideals within society. Art Nouveau thus employed the nude to represent complex manifestations of modernity. Three diverse cases provide the subjects of each chapter. All explore modernism through objects and interiors, in public and private environments, and each connects the decorative arts with accounts of European modernism in …

Catching All Passions In His Craft Of Will: Portraits And Pater In Oscar Wilde’S “The Portrait Of Mr. W. H.”, Rebecca E. Jones

Theses and Dissertations

This thesis examines Oscar Wilde’s “The Portrait of Mr. W. H.” as the product of Wilde’s long interest in critic Walter Pater’s literature and scholarship. From its first iteration published in 1889, through Wilde’s ongoing revision and expansion into the version commonly anthologized today, “The Portrait of Mr. W. H.” is an evolving work that mirrors Wilde’s enduring relationship with the art and ideas of his former teacher. This relationship is explored in three contexts: Pater’s contribution to Wilde’s understanding of the Renaissance period; the steady influence of Pater’s ideas and persona on Wilde’s other major works from the period …

The Color Of Memory: Reimagining The Antebellum South In Works By James Mcbride Through The Use Of Free Indirect Discourse, Janel L. Holmes

Theses and Dissertations

This thesis examines the use of interior narrative techniques such as free indirect discourse and internal monologue in two of James McBride’s neo-slave narratives, Song Yet Sung (2008) and The Good Lord Bird (2013). Very limited critical attention has been given to these neo-slave narratives that illustrate McBrides attention to characterization and focalized narration. In these narratives McBride builds upon the revelations he explores in his bestselling memoir, The Color of Water (1996, 2006), where he learns to disassociate race and character. What he discovers about not only his mother, but also himself, inspires his re-imagination of the people who …

The Entertainment Is Terrorism: The Subversive Politics Of Doing Anything At All, Joe Woods

Theses and Dissertations

When the body is observed through a certain combination of technologies, there can be subversive politics to doing anything at all. The nature of media and biopolitics has permitted for a set of systems aimed at total control of the human body; a power which can permeate all facets of life. This thesis is a collection of essays which argues that speculative fiction contains multitudes of approaches to biopolitical discourse, permitting the reader of the text to approach politics from their own set of experiences, but not allowing the political to be ignored. These chapters contain three separate but interrelated …

Sulfated Dehydropolymer Of Caffeic Acid For Repair Of Lung Damage And Emphysema, Tien M. Truong

Theses and Dissertations

The complex pathobiologic mechanisms of emphysema are not fully understood, leaving this deadly disease without effective pharmacotherapy for a cure. This project hypothesized that the sulfated dehydropolymer of caffeic acid (CDSO3) exhibits Fe²⁺ chelation-based hypoxia inducible factor-1a (HIF-1a) up-regulatory protective activities against in vitro emphysematous cell death and for in vivo reversal of emphysema induced with SU5416, a vascular endothelial growth factor blocker.

Using in vitro chromogenic competitive inhibition assays, CDSO3 was shown to chelate Fe²⁺ (IC₅₀ of 23 µM), but not Fe³⁺ ions. The trypan blue exclusion and lactate

dehydrogenase assays were then employed to …

Methcathinone Analogue Activity At The Human Serotonin Transporter, William Drake Varn

Theses and Dissertations

In the last few years, there has been continued concern about synthetic drug abuse in both the United States and worldwide. Small adjustments in drug compound structure often allow synthetic drug makers to manufacture a legal product that can produce the same highs as illegal counterparts. Unfortunately, this is happening faster than the government can outlaw the drug compounds, and a wide variety of synthetics are now appearing on the street. This study evaluated the effects on the human serotonin transporter of six different 4-para substituted methcathinone compounds. Using a Xenopus oocyte model, the efficacy of each MCAT analogue …

Examining Genetic And Environmental Influences On Alcohol Use And Externalizing Behaviors In African American Adolescents, Neeru Goyal

Theses and Dissertations

The aim of this thesis is to expand our understanding of the etiology of alcohol misuse and related disorders in African Americans using genetically informative study designs. Specifically, we take advantage of the candidate-gene approach and polygenic score analysis to extend the literature specific to African American populations. Chapter 2 explores gene x environment (GxE) interactions through the candidate gene approach to explore the relationship between two genes chosen on their potential relevance to stress response and adolescent alcohol use and misuse, among African American youth living in highly impoverished neighborhoods, as moderated by stressful life events. Chapter 3 implements …

I Reach Toward The Ground I Reach Toward Space, Kristen A. Sanders

Theses and Dissertations

Imagine one million and six hundred thousand years ago — a prehistoric character points. There in the moistened soil, the hominid Homo habilis draws a line, inscribing with her finger the first mark. At the core of my work is a meditation on this very scene as the otherwise unknowable origins of image making. And at the center of this discovery is a prehistoric woman — a previously invisible character within human evolution. My work aims to insert these characters into the origins of tool use, image making, and other supposed markers of humanness while also examining historical representations of …

Sweaty Mother Slow Groove, Devin Kylie Harclerode

Theses and Dissertations

Sweaty Mother Slow Groove is an engagement in magical thinking that proposes a displacement of swamp methodologies into the virtual realm, existing during the fourth wave. In doing so the cyborg and goddess are united in a re-routing of essentialism and the neo-liberal domination of technology. The metaphorical swamp is the possibility of a mushy danger zone that harnesses the absorption of an unwanted space: a disintegration of the binary and the soft-coded awareness of the body as a process, not a site.

The Exchange: Curating Authenticity + Interaction, Sarah Webb

Theses and Dissertations

For a physical space to have an emotional impact on those who experience it, we must consider the connection and relationship between objects and experience, and how people make individual connections to insentient places. It is this symbiotic relationship that allows a building or space to attain a “soul”.

Through the adaptive re-use of a Richmond, Virginia building, this thesis project explores strategies of staging physical interaction and organic experiences through art and culture in the context of a mixed-use niche hotel.

It's Slapstick Design, Thanks! Wait! No "Thanks"- Just Slapstick Design ..., Curtis A. Bailey

Theses and Dissertations

I'm arguing for an approach to generating graphic form based on slapstick. Slapstick is a genre of physical comedy involving humorous portrayals of clumsiness, mistakes, and nonsense. I investigate it as an approach to form, content, process, and communication. These methods were used to construct an immersive installation loosely based on The Tibetan Book of the Dead.

Graphic design is expected to function. It does this by conditioning users to its particular patterns or by skillfully implementing familiar patterns. A slapstick approach to graphic design is valuable as a critical tool for disrupting conditioned experiences for promoting empathy by relating …

No Woman Is An Island, Adele Ball

Theses and Dissertations

We have gathered the following pages to archive our time here in Richmond, Virginia. We have been here for two years, growing slowly, moving when needed to new anchorholds to avoid detection or arrest. We scrutinize the urban environment like modern archeologists. We collect stories and speculate about new uses of old things. It is imperative to be resourceful here, and we do so out of necessity but also in the spirit of practice. These pages were made en route, each an exploration of the tools at hand when on the move. The method of creation is just as important …

Choreographic Space, Kelsey Sheaffer

Theses and Dissertations

This thesis, Choreographic Space, and accompanying exhibit is an arrangement of contemporary work being done in the cross-over between movement, drawing, sound and architecture. The thesis develops a lineage of choreographic thinking through a fissure in the classification of a dance as necessarily the body in motion. Through the link of the “choreographic object,” Choreographic Space asks how an interdisciplinary exploration of the principles of movement can reveal novel ways to think about the body in space.

Monitor, Shana Hoehn

Theses and Dissertations

Descriptions of a scene interwoven with thoughts on how aesthetics sets behavioral expectations, observation as proof, views from a single perspective, casting of characters, spaces that evoke complicated histories, and my own history.

Fundamental Units, Ryan M. Flores

Theses and Dissertations

Surface, Skin, Facade, Countenance, Resistance, Chinchorro Mummies,Touch, Mark Making, Residue, Intuition, Repression, Indigestion, Disassemble, Blighted,Components, Body as Containers, Levels of Hierarchy, Absorption, Plastination, Modernism and Containment, Vanitas, Disembodied, Embodiment, Fragmentation, Arches, Gothic, Typology, Stacking of Cultures, Slippage.

Death Vegas Valley, Eric M. Diehl

Theses and Dissertations

My work explores hallucinatory landscapes of the US American West by using a combination of painting styles outside of the Western painting canon. I cross-reference painting and cinema, interweaving video, acrylic paint and the panorama to create a satirical homage to the history and present state of the USA. However, through an earnest devotion to the medium - both painting and cinema - I find my criticisms also yearn to hold onto a belief in a myth I know to be false.

This is an American History conversation about artifice and consumerism through advertising. I use Las Vegas and the …