Digital Commons Network^™

The Identification Of The Direct And Indirect Pathways Through Which Leptin Facilitates Synaptic Plasticity In The Hippocampus, Catherine Van Doorn

Theses and Dissertations

Leptin, a peptide synthesized by adipocytes in the periphery, has been shown to play significant roles in feeding and energy expenditure mediated by the hypothalamus. Growing evidence supports the role of leptin in influencing synaptic plasticity in the hippocampus in that leptin dose-dependently enhances LTP, alters morphology and neurogenesis, facilitates spatial learning and memory, as well as memory retention. Models of leptin deficiency and resistance have further supported the importance of leptin in synaptic plasticity by exhibiting deficits in electrophysiological, morphological and behavioral tests that are improved after leptin restoration. The effects of leptin when applied directly into the hippocampus …

Neural And Behavioral Correlates Of Planning During Vocal Motor Control, Stacey Jean Sangtian

Theses and Dissertations

Speech is a goal-directed movement developed to serve the purpose of human communication. During speech, the brain simultaneously coordinates complex motor behavior and sensory information for planning, execution and online monitoring for error correction in order to ensure accurate delivery of the vocal messages. Models of speech production and voice motor control have adapted the principles of the internal forward model to explain that speech sound production is controlled by comparison of internal predictions with sensory feedback. Growing evidence from studies conducted in speech and limb motor movement indicate that these sensory predictions and responsive commands are loaded in anticipation …

Role Of Altered Gut Microbiota In Tumor Development, Mucus Production And Inflammation In Apc Min/+ Mouse Model, Kamaljeet Kaur

Theses and Dissertations

The microbiome forms an integral part of the gut microenvironment. Once ignored, the topic has gained momentum in research during the past decade, where studies have strongly suggested the association of microbiota with health and a misbalance thereof, to many disease conditions ranging from inflammation and colitis to diabetes, obesity and colon cancer. The Human Microbiome Project (HMP, NIH common fund - 2008) has used a variety of high throughput analyses in order to study gut microbiota in health. The consortium has so far been able to isolate and characterize more than 1,300 reference bacterial strains from the human body. …

Modern Estimation Problems In Group Testing, Md Shamim Sarker

Theses and Dissertations

In the simplest form of group testing, pools are formed by compositing a fixed number of individual specimens (e.g., blood, urine, swab, etc.) and then the pools are tested for a binary characteristic, such as presence or absence of a disease. Group testing is commonly used to screen for a variety of sexually transmitted diseases in epidemiological applications where the main goal is to increase testing efficiency. In this dissertation, we study three estimation problems that are motivated by real-life applications. We propose new methods to model group testing data for both single and multiple infections. In the first problem, …

Investigating Cross Talk Between The High And Low Iron Sensors In Saccharomyces Cerevisiae, John S. Hepburn

Theses and Dissertations

Iron is essential for nearly all life on earth. Cells must maintain sufficient levels of this important nutrient due its function as a central co-factor for many cellular processes. Although iron is extremely plentiful, its poor solubility requires specialized import. On the other side, too much iron inside the cell can lead to the generation of reactive oxygen species (ROS), which have deleterious effects on many cellular functions and have been implicated in a wide variety of diseases such as cancer and Alzheimer’s disease. Using the model eukaryote Saccharomyces cerevisiae, or budding yeast, our research group has accomplished much in …

Deserts Will Bloom: Atomic Agriculture And The Promise Of Radioactive Redemption, Chris Fite

Theses and Dissertations

This paper examines the rhetorical and visual strategies used in marketing atomic agriculture to the American public from the 1940s to the 1960s. The term “atomic agriculture” refers to various agricultural research programs that used radioactive materials, particularly radioisotope tracing and mutation breeding. In print and on screen, atomic boosters from government and industry offered the promise of a better world made possible by applying atomic energy to agriculture. I argue that the proponents of atomic agriculture combined futurism and nostalgia to create a techno-pastoral vision. They hearkened back to the nineteenth century while simultaneously imagining a bright postwar future. …

Development Of Reactions And Methods For Labeling Biomolecules In Cells, Enoch Agbesi Adogla

Theses and Dissertations

The complexity of cellular environments presents a challenge to efforts at studying and elucidating biomolecules in cells. Covalent ligation of biomolecules with fluorescent molecules that have a reactive functional group that can react with a complementary functional group on biomolecules is one way that has been utilized to study biomolecules. It enables visualization and tracking of ligation and transportation of molecules within the cell. However, the myriad of functional groups within the cell makes achieving selectivity difficult. To circumvent this problem, reactions that do not interfere with biology have been developed. These reactions, referred to as bioorthogonal reactions must be …

Investigating The Function Of Aintegumenta-Like6 (Ail6) In Arabidopsis Flower Development, Han Han

Theses and Dissertations

AINTEGUMENTA (ANT) and AINTEGUMENTA-LIKE6 (AIL6) encode related transcription factors with partially overlapping roles in floral organ development in the model plant Arabidopsis thaliana. ANT and AIL6 do not make equivalent contributions to these processes. Loss of ANT function by itself results in smaller flowers, demonstrating that the role of ANT in organ size control cannot be provided by AIL6. Loss of AIL6 function on its own has no phenotypic consequences indicating that all of its roles in flower development can be provided by ANT or some other genes. To further probe the function of AIL6 in flower development, we investigated …

Relationships Of Chronotype, Social Jetlag, Sleep, Physical Activity And Diet With Mood, Perceived Stress, Obesity, Blood Pressure And Prostate Cancer, Daria M. Mcmahon

Theses and Dissertations

Background: Chronotype, social jetlag, poor sleep, proinflammatory diet, and low physical activity have been associated with increased risk of chronic diseases: obesity, diabetes, cardiovascular disease, depression and cancer. Yet the relationships between these factors have not been extensively investigated in prospective studies.

Methods: Two studies were conducted. The first study followed 390 healthy men and women ages 21-35 for two years. Social jetlag [SJL] and sleep measures (total sleep time [TST], sleep onset latency [SOL], wake after sleep onset [WASO]), sleep efficiency [SE]), were derived from physical activity personal (armband) monitoring. The participants wore the armband for 4-10 …

The Effect Of Arsenic On Type 2 Diabetes And Inflammation, Kayla Penta

Theses and Dissertations

Arsenic, a ubiquitous environmental contaminant, has been shown to cause a number of health effects. At high concentrations the inorganic form is a well-known toxin, but at lower concentrations the effects range from various cancers, to cardiovascular disease and type 2 diabetes. At higher concentrations of arsenic (500- 1000μg/L) there have been epidemiological studies conducted demonstrating an increased risk in the development of type 2 diabetes with this exposure. At lower levels of arsenic exposure (<500 μg/L) the epidemiological results are inconclusive. Arsenic is also an immunotoxicant, meaning that it will cause changes in the immune response. The changes in the immune response will vary depending on a number of variables, including amount of arsenic exposure, forms of exposure and route of exposure. We wanted to determine if arsenic could modulate the immune system, and if this change could lead to an increase in susceptibility to type 2 diabetes development. We chose to examine this in C57BL/6 and db/+ mice – two non-susceptible strains. After 8 weeks (4 weeks old to 12 weeks old) of low dose inorganic arsenic exposure (50 μg/kg or 500 μg/kg) we evaluated changes in body composition, glucose tolerance and immune response. We saw that there were differences based on sex, genotype and treatment group present after the 8-week treatment period in body composition, while there were minimal changes in glucose tolerance. Finally, the immune response showed great variability depending on sex, genotype and treatment group. This project has demonstrated that while we are trying to compare differences in in vivo and epidemiological studies to find a link between arsenic and type 2 diabetes, there may be deeper levels of complications based on individual variability to arsenic exposure.

Public Perceptions Of Genetically Modified Food On Social Media: A Content Analysis Of Youtube Comments On Videos, Nanlan Zhang

Theses and Dissertations

Controversy about genetically modified (GM) food prevails on social media. Discussion about GM food includes the implementation of mandatory labeling as well as public concerns about potential health hazards posed by GM food. Previous studies mainly focus on traditional press and broadcast media, few investigate such controversial topics on social media. Interested in public opinion about this issue and possible influences of social media on public opinion, this study uses quantitative content analysis to examine the characteristics of user comments on a specific social media platform, YouTube. The purpose of the study is to investigate YouTube comments from several aspects, …

Ashley's Sister, Christina Phillips

Theses and Dissertations

This nonfiction project is a memoir, constructed in sections, about my relationship with my sister with an intellectual disability.

Preservice Teachers' Beliefs About Play In Kindergarten, Michelle Taylor Clevenger

Theses and Dissertations

The purpose of this research was to gain insight into preservice teachers’ beliefs about play in kindergarten, and explore differences in beliefs about play between teachers just beginning their education program and those who are completing their degree. The study examined the beliefs of two groups of preservice teachers: one at the beginning of their early childhood education program (beginning students) and one at the end (teacher candidates). This study used a mixed methods approach including a survey with both Likert scale and open-ended questions as well as individual interviews. Data were collected at the beginning of the fall semester …

Ain't Misbehavin': Phenomenological Inquiry Into Black Male Experiences Of School, Kelly N. Ferguson

Dissertations

Disproportionate representation of Black male students in subjective areas of discipline has long been documented. Research indicates that racial disparities in discipline have been found with insufficient evidence to support that Black or other minority students are simply misbehaving more than others. Differences in cultural orientations between Black males and their predominantly White and female teachers have been linked to bias that disproportionality impacts discipline for these students. Through phenomenological inquiry, this study explored the schooling, professional, and mentoring experiences of successful Black men to better understand how schools and communities contribute to their resilience. This research sought to uncover …

Mechanism By Which Commensal Bacteria Limit Inflammation, Mallory Paynich

Dissertations

Trillions of bacteria live within the gastrointestinal tract and are critical for maintaining intestinal homeostasis; however, the mechanisms utilized by specific bacterial molecules to contribute to homeostasis are not well understood. We utilize a mouse model in which a single oral dose of the probiotic, Bacillus subtilis, protects mice from acute colitis induced by the enteric pathogen Citrobacter rodentium. Our goal is to elucidate the mechanism by which B. subtilis prevents inflammation.

We identified exopolysaccharides (EPS) to be the active molecule of B. subtilis, and a single dose of EPS protects mice from disease. EPS binds F4/80+CD11b+ peritoneal macrophages, and …

Keeping Schools Catholic: Discipleship As Paradigm For Administrators, Teachers, Staff And Students, James (Deporres) Charles Durham

Dissertations

Of the many accomplishments of the Catholic Church in the United States, one of the most known is the Catholic School System. In 1965, over 4.4 million children were educated in the 10,667 Catholic elementary schools, and almost 700,000 in the 1,527 Catholic high schools (Center for Applied Research for the Apostolate, 2013). The mission of the school was embodied, literally, in these skilled male and female religious who taught in these schools. But the number of male and female religious was not sustained, leading to the question of how the religious mission of the school will continue. The number …

Emature: Intergenerational Networks In Digital Media Spaces, Andras Lukacs

Dissertations

While the literature on adolescent usage of the Internet and mobile communication technology is burgeoning, the technological affordance of increased intergenerational contacts and intergenerational friendships received less attention. This project documents how intergenerational virtual networks operate in one particular massively multiplayer online game (World of Warcraft) from the standpoint of adolescent players. Online social worlds are similar to offline settings in a lot of ways. Users must obey certain behavioral standards and follow established rules and moral codes to participate. Despite accounts of online democracy and networked individualism, control and authority is central to the functioning of these environments. Power-relationships …

Development Of "Inside-Out" Pegylated Crosslinked Hemoglobin Polymers: Novel Hemoglobin-Based Oxygen Carriers (Hboc), Kyle Douglas Webster

Dissertations

The development of an effective blood substitute is urgent due to increasingly common blood shortages, the need to type-match donated blood, and communicable diseases (e.g. HIV) posing risks for blood transfusions around the world. There have been many attempts at creating hemoglobin-based oxygen carriers (HBOC) using a variety of techniques centered around the use of polyethylene glycol (PEG) conjugated to hemoglobin (Hb) tetramers. A novel method, “Inside-Out” PEGylation, has been developed by our lab to produce a polyethylene glycol crosslinked hemoglobin (PEG XL-Hb) polymer. This method utilizes a single PEG backbone that is surrounded by multiple proteins, instead of covering …

Cardiac Calcium Atpase Dimerization Measured By Fluorescence Resonance Energy Transfer And Chemical Cross-Linking, Daniel Blackwell

Dissertations

The cardiac sarco/endoplasmic reticulum calcium ATPase (SERCA) establishes the intracellular calcium gradient across the sarcoplasmic reticulum membrane. It has been proposed that SERCA forms homo-oligomers that increase the catalytic rate of calcium transport. We investigated SERCA oligomerization in rabbit left ventricular myocytes using a photoactivatable cross-linker. Western blotting of cross-linked SERCA revealed higher molecular weight species consistent with SERCA oligomerization. Fluorescence resonance energy transfer (FRET) measurements in cells transiently transfected with fluorescently-labeled SERCA2a revealed that SERCA readily forms homo-dimers. These dimers formed in the absence or presence of the SERCA regulatory partner, phospholamban (PLB) and were unaltered by PLB phosphorylation …

Kant, The Natural Law, And The Question Of Normativity In Catholic Ethics, John Robert Crowley-Buck

Dissertations

This dissertation explores the question of normativity in 21st century Catholic ethical reflection. While, today, the natural law tradition frames and founds the normative question in Catholic ethics in terms of the imago Dei, this project explores the moral philosophy of Immanuel Kant and the challenge it presents to the natural law's claim to normativity. Specifically, this dissertation argues that in Kant's ethics, the normative question is answered in terms of the dignity of humanity, rather than the imago Dei, and that the dignity of humanity is a more relevant, shareable, and normative grounding for ethical reflection. To this end, …

A Higher Law: Transatlantic Revolution And Antislavery Radicalism In Early America, 1760-1800, Anthony Di Lorenzo

Dissertations

During the Age of Revolution, abolitionist ideas interacted with notions of liberty, independence, and equality. Although slavery often served as a metaphor, in opposition to freedom, it also had tangible meanings for the enslaved. This study traces the development of revolutionary beliefs that connected reformers and abolitionists across the Atlantic world, as well as the rise of conservative ideologies that divided them. Democratic politics, religious enthusiasm, and abolitionism converged in the late eighteenth century, with significant implications for antislavery efforts. The French Revolution, in particular, represented the culmination of radical Enlightenment ideals and emboldened democrats in the United States, contributing …

Clusters Of Alpha Satellite On Human Chromosome 21 Are Dispersed Far Onto The Short Arm And Lack Ancient Layers, William Ziccardi, Chongjian Zhao, Valery Shepelev, Lev Uralsky, Ivan Alexandrov, Tatyana Andreeva, Evgeny Rogaev, Christopher Bun, Emily Miller, Catherine Putonti, Jeffrey Doering

Bioinformatics Faculty Publications

Human alpha satellite (AS) sequence domains that currently function as centromeres are typically flanked by layers of evolutionarily older AS that presumably represent the remnants of earlier primate centromeres. Studies on several human chromosomes reveal that these older AS arrays are arranged in an age gradient, with the oldest arrays farthest from the functional centromere and arrays progressively closer to the centromere being progressively younger. The organization of AS on human chromosome 21 (HC21) has not been well-characterized. We have used newly available HC21 sequence data and an HC21p YAC map to determine the size, organization, and location of the …

Beyond ‘Innocents Abroad’: Reflecting On Sustainability Issues During International Study Trips, Anne H. Reilly, Mary Ann Pauline Mcgrath, Kristine Reilly

School of Business: Faculty Publications and Other Works

With ecosystems and populations in many regions threatened by rapid development, sustainability is a critical component for businesses in mature markets and emerging economies alike. The International Association of Jesuit Business Schools notes that global sustainability involves a broad set of interconnected issues ranging from environmental preservation to social justice to desirable production and consumption patterns. Jesuit business schools are uniquely positioned to address sustainability issues with their focus on teaching managerial content in tandem with corporate social responsibility. Further, the Ignatian Pedagogy Paradigm of experience, reflection, and action would suggest that business students may benefit from reflective observation in …

Land-Use And Algal Species Influence Bacterial Community Composition And Activity In Streams, Miguel Rojas

Master's Theses

Microbial biofilms can be found on virtually all submerged surfaces in aquatic habitats, including rivers and streams. Biofilms are complex, taxonomically diverse communities of microorganisms (including algae and bacteria) that colonize solid surfaces and support a wide range of metabolic activities. Biofilms are critical components of river and stream ecosystems because they are key drivers of nutrient cycling processes (including nitrogen cycling) and because they serve as an important food source for higher trophic levels. Biofilms in urban streams are subject to a number of anthropogenic stressors, but research on the ecology of urban biofilms and their response to stressors …

A Fire That Could Not Be Extinguished: Sovereignty And Identity In The Pokagon Band Of Potawatomi Indians, 1634-1994, Melisa Cushing-Davis

Dissertations

American Indian sovereignty and identity, the rights of political and economic self-determination and self-definition, have suffered numerous blows since the time of First Contact. Under the policies of various European nations, and finally the United States, indigenous residents of North America have struggled to maintain political independence, as well as cultural and social integrity, while adapting to changing conditions over which they found themselves, most often, to have little direct control. Such is the history of the Potawatomi people. This dissertation examines the historic responses to these continuing challenges of one band of Potawatomi, the Pokagon of southwestern Michigan. It …

Simulation Learning And Transfer To The Clinical Environment In Undergraduate Nursing Students, David A. Miles

Dissertations

Simulation learning is an integral component of many undergraduate nursing programs throughout the country. Experiential learning through simulation allows students to improve their cognitive, affective, and psychomotor skills. Some clinical experiences lack significant practice of clinical skills for students; including the inability to assume the role of the nurse. A recent literature review revealed a need to advance the understanding of simulation learning and transfer; with many questions still remaining unanswered. The aim of this study was to conceptualize the process by which simulation learning transfers to the clinical environment in undergraduate nursing students. Twenty-five, fourth-year traditional nursing students, who …

Badr Al-Dīn Ibn JamāʿAh And The Highest Good Of Islamic Education, Omar Qureshi

Dissertations

The secularization of the academy thesis refers to the phenomenon of Protestant colleges and universities starting out as identifiable religious institutions of education now being places hostile, not only to Christianity, but religion in general. This has raised much discussion among leaders, faculty members, and students of religious educational institutions as to what is and what constitutes the identity of their respective institutions. It is in this context that we witness the rise in the establishment of Islamic schools in the North America. This context has generated many questions from the various stakeholders on the question of what the term …

Comparing Predominant Spiritual Approaches In A Chicago Archdiocese Vicariate Using The Spirituality Opionnaire And Assessing Its Reliability, John Peter Segvich

Dissertations

John P. Segvich

Loyola University Chicago

COMPARING PREDOMINANT SPIRITUAL APPROACHES IN A CHICAGO ARCHDIOCESE VICARIATE USING THE SPIRITUALITY OPIONNAIRE AND ASSESSING ITS RELIABILITY

This study determined the predominant spiritual approach among the principals and teachers of each school and deanery within Vicariate 5 of the Archdiocese of Chicago, Office of Catholic Schools. This study examined the predominant spiritual approach at the Vicariate level, school level, and deanery level, for Vicariate 5 of the Archdiocese of Chicago, Office of Catholic Schools.

This study used the spirituality opionnaire developed by John Haughey, SJ, and Fr. Anthony Ciorra. The spirituality opionnaire was derived …

Examining The Effects Of Anti-Nogo A Immunotherapy On Post-Stroke Neurogenesis In The Adult Rat, Daniel Shepherd

Dissertations

Stroke is a leading cause of adult disability with no pharmacological treatments to restore lost function. Our laboratory has shown that treatment with neutralizing antibodies against the neurite growth-inhibitory protein Nogo-A improves sensorimotor and cognitive recovery after stroke in adult and aged rats. This recovery is paralleled by increased dendritic and axonal plasticity in anti-Nogo-A-treated rats. Neurogenesis, an alternate form of plasticity involving the de novo production of new neurons, may contribute to post-stroke neural repair. While previous studies have found roles for Nogo-A in adult neurogenesis, neurogenesis has not been investigated after stroke and anti-Nogo-A treatment. The goal of …

Critical Role Of C-Jun N-Terminal Kinase Signaling In Binge Alcohol-Driven Atrial Arrhythmic Remodeling, Jiajie Yan Yan

Dissertations

Holiday Heart Syndrome (HHS) is cardiac arrhythmia induced by binge alcohol consumption, a drinking pattern affects 38 million adults in our society. Atrial fibrillation (AF) is the most frequently diagnosed arrhythmia in HHS and causes significant morbidity and mortality. Despite the intensive prevention effort nationwide, the binge drinking population keeps rising. However, no effective treatment strategies are available for binge drinking induced AF due to the unknown underlying mechanisms. In this thesis, I aim to elucidate the mechanisms of binge alcohol-promoted atrial arrhythmogenicity by using a mouse model of repeated binge alcohol exposure established in the Ai Lab.

The Ai …