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Role For Migratory Wild Birds In The Global Spread Of Avian Influenza H5n8

United States Department of Agriculture Wildlife Services: Staff Publications

Avian influenza viruses affect both poultry production and public health. A subtype H5N8 (clade 2.3.4.4) virus, following an outbreak in poultry in South Korea in January 2014, rapidly spread worldwide in 2014–2015. Our analysis of H5N8 viral sequences, epidemiological investigations, waterfowl migration, and poultry trade showed that long-distance migratory birds can play a major role in the global spread of avian influenza viruses. Further, we found that the hemagglutinin of clade 2.3.4.4 virus was remarkably promiscuous, creating reassortants with multiple neuraminidase subtypes. Improving our understanding of the circumpolar circulation of avian influenza viruses in migratory waterfowl will help to provide …

Prioritizing Management And Research Actions Against Invasive Reptiles In Florida: A Collaboration By An Expert Panel, Richard M. Engeman, Michael L. Avery

United States Department of Agriculture Wildlife Services: Staff Publications

Florida has more introduced animals than any other region of the U.S. and also ranks high in this respect globally. Given Florida's climate, it is no coincidence that a large proportion of Florida’s invasive vertebrate species are reptiles and amphibians. Exotic snakes, lizards, frogs, turtles, and crocodilians are all breeding in Florida. The largest snakes in Florida are constrictors from other continents, and the five largest lizard species breeding in Florida are from Africa, South America, and Central America. Establishment of non-native reptiles and amphibians has been documented in Florida for over 135 years, and the rate of invasive reptile …

Antigenic Characterization Of H3 Subtypes Of Avian Influenza A Viruses From North America, Elizabeth Bailey, Li-Ping Long, Nan Zhao, Jeffrey S. Hall, John A. Baroch, Jacqueline M. Nolting, Lucy Senter, Frederick L. Cunningham, G. Todd Pharr, Larry Hanson, Richard Slemons, Thomas J. Deliberto, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Besides humans, H3 subtypes of influenza A viruses (IAVs) can infect various animal hosts, including avian, swine, equine, canine, and sea mammal species. These H3 viruses are both antigenically and genetically diverse. Here, we characterized the antigenic diversity of contemporary H3 avian IAVs recovered from migratory birds in North America. Hemagglutination inhibition (HI) assays were performed on 37 H3 isolates of avian IAVs recovered from 2007 to 2011 using generated reference chicken sera. These isolates were recovered from samples taken in the Atlantic, Mississippi, Central, and Pacific waterfowl migration flyways. Antisera to all the tested H3 isolates cross-reacted with each …

Effects Of Visual Obstruction, Prey Resources, And Satiety On Bird Use Of Simulated Airport Grasslands, Bradley F. Blackwell, Thomas W. Seamans, Kimberly Linnell, Lisa Kutschbach-Brohl, Travis L. Devault

United States Department of Agriculture Wildlife Services: Staff Publications

Grasslands represent 39%–50% of U.S. airport properties, and a recent management framework recommended exploiting both antipredator behaviours and food resources in airport grasslands to curb use by birds considered hazardous to aviation safety. We evaluated framework predictions empirically by exposing unsated and sated brown-headed cowbirds (Molothrus ater) to visually obstructive (∼13-cm vegetation height; tall), higher-risk plots versus unobstructive (height; short) plots, and relative to prey resources. We predicted that 1) unsated birds (unfed since the previous day) would be present in greater numbers and forage more in short than tall vegetation plots 24 h post-mowing because of invertebrate …

Widespread Detection Of Antibodies To Eastern Equine Encephalitis, West Nile, St. Louis Encephalitis, And Turlock Viruses In Various Species Of Wild Birds From Across The United States, Kerri Pedersen, David R. Marks, Eryu Wang, Gillian Eastwood, Scott C. Weaver, Samuel M. Goldstein, David R. Sinnett, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

Wild birds serve as amplifying hosts for many arboviruses, and are thought to be responsible for introducing these viruses into new areas during migration as well as reintroducing them to places where winter temperatures disrupt mosquito-borne transmission. To learn more about four mosquito-borne arboviruses of concern to human or animal health, we tested sera from 997 wild birds of 54 species and 17 families across 44 states of the United States collected from January 1, 2013, through September 30, 2013. Samples were tested for antibody against eastern equine encephalitis, St. Louis encephalitis, West Nile, and Turlock viruses using plaque reduction …

Weak Support For Disappearance And Restricted Emergence/Persistence Of Highly Pathogenic Influenza A In North American Waterfowl, Andrew M. Ramey, Erica Spackman, Mia Kim-Torchetti, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

Krauss et al. (1) use lack of detection of highly pathogenic (HP) H5 clade 2.3.4.4 (henceforth “H5”) influenza A viruses (IAVs) from >22,000 wild bird samples collected in North America in 2014–2015 to argue that HP H5 IAVs disappeared from waterfowl and that unresolved mechanisms restrict emergence and perpetuation of HP IAVs in natural reservoir species. Here we offer an alternative interpretation.

Use Of Predator Hair To Enhance Perceived Risk To White-Tailed Deer In A Foraging Context, Thomas W. Seamans, Bradley F. Blackwell, Kimberly E. Linnell

United States Department of Agriculture Wildlife Services: Staff Publications

Deer react to predator scent in varying degrees even when exposed to unknown predators. This response could be genetically based and maintained as long as the population is exposed to predation. We tested whether predator scent in the form of hair would enhance perceived risk and serve as a foraging repellent to free-ranging white-tailed deer (Odocoileus virginianus). During the winters of 2013 and 2014, we quantifi ed alert behaviors and consumption of whole-kernel corn in response to current (coyote [Canis latrans]) and extirpated (bobcat [Lynx rufus]; black bear [Ursus americanus]) predator species …

Repellent Application Strategy For Wild Rodents And Cottontail Rabbits, Scott J. Werner, Shelagh T. Deliberto, Roger A. Baldwin, Gary W. Witmer

United States Department of Agriculture Wildlife Services: Staff Publications

Effective chemical repellents and repellent application strategies are needed to manage damages caused by wild rodents and rabbits to agricultural resources. For the purpose of comparatively investigating the behavioral response of wild rodents and rabbits to a chemical repellent, we experimentally evaluated the concentration-response relationship of an anthraquinone-based repellent in California voles (Microtus californicus Peale), Richardson’s ground squirrels (Urocitellus richardsonii Sabine), deer mice (Peromyscus maniculatus Wagner) and cottontail rabbits (Sylvilagus audubonii Baird) in captivity. We observed 52–56% feeding repellency for whole oats treated with 10,800ppm anthraquinone or 18,500ppmanthraquinone in mice and squirrels, and 84–85% repellency for …

Multi-Isotopic (Δ2H, Δ13C, Δ15N) Tracing Of Molt Origin For Red-Winged Blackbirds Associated With Agro-Ecosystems, Scott J. Werner, Keith A. Hobson, Steven L. Van Wilgenburg, Justin W. Fischer

United States Department of Agriculture Wildlife Services: Staff Publications

We analyzed stable-hydrogen (δ²H), carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope ratios in feathers to better understand the molt origin and food habits of Red-winged Blackbirds (Agelaius phoeniceus) near sunflower production in the Upper Midwest and rice production in the Mid-South of the United States. Outer primary feathers were used from 661 after-second-year (ASY) male blackbirds collected in Minnesota, Montana, North Dakota and South Dakota (spring collection), and Arkansas, Louisiana, Mississippi, Missouri and Texas (winter collection). The best-fit model indicated that the combination of feather δ²H, δ¹³C and δ …

Immunotherapy Using Algal-Produced Ara H 1 Core Domain Suppresses Peanut Allergy In Mice, James A. Grgeory, Ariel Shepley-Mctaggart, Michelle Umpierrez, Barry K. Hurlburt, Soheila J. Maleki, Hugh A. Sampson, Stephen P. Mayfield, M. Cecilia Berin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates immunotherapy studies. Moreover, peanut immunotherapy is associated with frequent negative side effects and patients are often at risk of allergic reactions once immunotherapy is discontinued. Allergen-specific approaches using recombinant proteins are an attractive alternative because they allow more precise dosing and the opportunity to engineer proteins with improved safety profiles. We tested whether Ara h …

Evaluating Methods To Establish Habitat Suitability Criteria: A Case Study In The Upper Delaware River Basin, Usa, H. S. Galbraith, C. J. Blakeslee, J. C. Cole, C. A. Talbert, K. O. Maloney

United States Geological Survey: Staff Publications

Defining habitat suitability criteria (HSC) of aquatic biota can be a key component to environmental flow science. HSC can be developed through numerous methods; however, few studies have evaluated the consistency of HSC developed by different methodologies. We directly compared HSC for depth and velocity developed by the Delphi method (expert opinion) and by two primary literature meta-analyses (literature- derived range and interquartile range) to assess whether these independent methods produce analogous criteria for multiple species (rainbow trout, brown trout, American shad, and shallow fast guild) and life stages. We further evaluated how these two independently developed HSC affect calculations …

Interpreting And Predicting The Spread Of Invasive Wild Pigs, Nathan P. Snow, Marta A. Jarzyna, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

1. The eruption of invasive wild pigs (IWPs) Sus scrofa throughout the world exemplifies the need to understand the influences of exotic and nonnative species expansions. In particular, the continental USA is precariously threatened by a rapid expansion of IWPs, and a better understanding of the rate and process of spread can inform strategies that will limit the expansion.

2. We developed a spatially and temporally dynamic model to examine three decades (1982– 2012) of IWP expansion, and predict the spread of IWPs throughout the continental USA, relative to where IWPs previously inhabited. We used the model to predict where …

Urolithin A, C And D, But Not Iso-Urolithin A And Urolithin B, Attenuate Triglyceride Accumulation In Human Cultures Of Adipocytes And Hepatocytes, Inhae Kang, Yongeun Kim, Francisco A. Tomás-Barberán, Juan Carlos Espín, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

Scope: Urolithins (Uro) are ellagic acid (EA)-derived metabolites produced by gut microbes. There is a growing interest in the biological activities of Uro. Our aim was to evaluate the impacts of Uro on regulating triglyceride (TG) accumulation using cultures of primary human adipocytes and hepatoma Huh7 cells.

Methods and Results: UroA, B, C, D, and iso-UroA, were used to determine the effect of Uro on adipogenesis and lipogenesis. Individual Uro (30 M) were added to human adipogenic stem cells (hASCs) during differentiation. UroA, C and D, but not iso-UroA and UroB, significantly inhibited new fat cell formation by decreasing TG …

Suppression Of Nlrp3 Inflammasome By Γ-Tocotrienol Ameliorates Type 2 Diabetes, Yongeun Kim, Wei Wang, Meshail Okla, Inhae Kang, Regis Moreau, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

The Nod-like receptor 3 (NLRP3) inflammasome is an intracellular sensor that sets off the innate immune system in response to microbial-derived and endogenous metabolic danger signals. We previously reported that γ-tocotrienol (γT3) attenuated adipose tissue inflammation and insulin resistance in diet-induced obesity, but the underlying mechanism remained elusive. Here, we investigated the effects of γT3 on NLRP3 inflammasome activation and attendant consequences on type 2 diabetes. γT3 repressed inflammasome activation, caspase-1 cleavage, and interleukin (IL) 1β secretion in murine macrophages, implicating the inhibition of NLRP3 inflammasome in the anti-inflammatory and antipyroptotic properties of γT3. Furthermore, supplementation of leptin-receptor KO mice …

Combined Iron Deficiency And Low Aerobic Fitness Doubly Burden Academic Performance Among Women Attending University, Samuel P. Scott, Mary Jane Desouza, Karsten Koehler, Laura E. Murray-Kolb

Department of Nutrition and Health Sciences: Faculty Publications

Background: Academic success is a key determinant of future prospects for students. Cognitive functioning has been related to nutritional and physical factors. Here, we focus on iron status and aerobic fitness in young-adult female students given the high rate of iron deficiency and declines in fitness reported in this population. Objectives: We sought to explore the combined effects of iron status and fitness on academic success and to determine whether these associations are mediated by cognitive performance.

Methods: Women (n = 105) aged 18–35 y were recruited for this cross-sectional study. Data were obtained for iron biomarkers, peak oxygen …

A Qualitative Phenomenological Exploration Of Teachers’ Experience With Nutrition Education, Elisha M. Hall, Weiwen Chai, Julie A. Albrecht

Department of Nutrition and Health Sciences: Faculty Publications

Background:

Nutrition education delivered by classroom teachers has become a popular intervention designed to combat childhood obesity. However, few qualitative studies have explored nutrition education with teachers.

Purpose:

The purpose of this study was to explore how elementary teachers describe their experience with nutrition education.

Methods:

A qualitative phenomenological approach was used. Semistructured interviews, observations, and document analysis were conducted with 10 teachers who delivered nutrition education in their classrooms. Inductive coding was used to determine invariant constituents, reduce constituents to categories, and cluster categories into themes. Reliability and validity were accomplished through intercoder agreement, audio recording, triangulation, bracketing, and …

Cranberries And Cancer: An Update Of Preclinical Studies Evaluating The Cancer Inhibitory Potential Of Cranberry And Cranberry Derived Constituents, Katherine M. Weh, Jennifer L. Clarke, Laura A. Kresty

Department of Nutrition and Health Sciences: Faculty Publications

Cranberries are rich in bioactive constituents reported to influence a variety of health benefits, ranging from improved immune function and decreased infections to reduced cardiovascular disease and more recently cancer inhibition. A review of cranberry research targeting cancer revealed positive effects of cranberries or cranberry derived constituents against 17 different cancers utilizing a variety of in vitro techniques, whereas in vivo studies supported the inhibitory action of cranberries toward cancers of the esophagus, stomach, colon, bladder, prostate, glioblastoma and lymphoma. Mechanisms of cranberry-linked cancer inhibition include cellular death induction via apoptosis, necrosis and autophagy; reduction of cellular proliferation; alterations in …

Pharmacological And Genetic Modulation Of Rev-Erb Activity And Expression Affects Orexigenic Gene Expression, Ariadna Amador, Yongjun Wang, Subhashis Banerjee, Theodore M. Kameneka, Laura A. Solt, Thomas P. Burris

Department of Nutrition and Health Sciences: Faculty Publications

The nuclear receptors REV-ERBα and REV-ERBβ are transcription factors that play pivotal roles in the regulation of the circadian rhythm and various metabolic processes. The circadian rhythm is an endogenous mechanism, which generates entrainable biological changes that follow a 24-hour period. It regulates a number of physiological processes, including sleep/wakeful cycles and feeding behaviors. We recently demonstrated that REV-ERB-specific small molecules affect sleep and anxiety. The orexinergic system also plays a significant role in mammalian physiology and behavior, including the regulation of sleep and food intake. Importantly, orexin genes are expressed in a circadian manner. Given these overlaps in function …

Modifying M-44s To Reduce Risk Of Activation By Swift Fox, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

M-44s are spring-powered sodium cyanide ejectors commonly used in the United States to manage livestock depredation by coyotes (Canis latrans). While highly selective for canids, improvements could reduce risk to nontarget canids, especially State-Endangered or -Threatened canids such as the swift fox (Vulpes velox). In 2012–2013, we tested M-44s set at modified heights to determine whether height modifications reduced risk to swift foxes without reducing activation rates by coyotes. We presented captive coyotes housed at the USDA National Wildlife Research Center’s Predator Research Facility, Millville, Utah, USA, with M-44s at various test heights to determine a …

Patuxent’S American Black Duck Studies From Chesapeake Bay To Maine And Beyond, Jerry R. Longcore

United States Geological Survey: Staff Publications

The information in this chapter draws on published literature and unpublished reports written by staff members of the U.S. Geological Survey, Patuxent Wildlife Research Center (Patuxent), during its 75-year history. Reports by Bureau of Biological Survey (Biological Survey) and U.S. Fish and Wildlife Service (USFWS) personnel are included because the research entity currently known as Patuxent was formerly administered by these agencies. Some of the cited reports were prepared by USFWS scientists while they were not working at Patuxent. Literature resulting from work at other Federal and State agencies and private and academic institutions that influenced research at Patuxent on …

Amino Acid Specific Stable Nitrogen Isotope Values In Avian Tissues: Insights From Captive American Kestrels And Wild Herring Gulls, C.E. Hebert, B.N. Popp, K.J. Fernie, C. Ka’Apu-Lyons, B.A. Rattner, N. Wallsgrove

United States Geological Survey: Staff Publications

Through laboratory and field studies, the utility of amino acid compound-specific nitrogen isotope analysis (AA-CSIA) in avian studies is investigated. Captive American kestrels (Falco sparverius) were fed an isotopically characterized diet and patterns in δ15N values of amino acids (AAs) were compared to those in their tissues (muscle and red blood cells) and food. Based upon nitrogen isotope discrimination between diet and kestrel tissues, AAs could mostly be categorized as source AAs (retaining baseline δ15N values) and trophic AAs (showing 15N enrichment). Trophic discrimination factors based upon the source (phenylalanine, Phe) and trophic (glutamic acid, Glu) AAs were 4.1 (muscle) …

Pranked By Audubon: Constantine S. Rafinesque’S Description Of John James Audubon’S Imaginary Kentucky Mammals, Neal Woodman

United States Geological Survey: Staff Publications

The North American naturalist Constantine S. Rafinesque spent much of the year 1818 engaged in a solo journey down the Ohio River Valley to explore parts of what was then the western United States. Along the way, he visited a number of fellow naturalists, and he spent more than a week at the Henderson, Kentucky, home of artist and ornithologist John James Audubon. During the succeeding two years, Rafinesque published descriptions of new species that resulted from his expedition, including eleven species of fishes that eventually proved to have been invented by Audubon as a prank on the credulous naturalist. …

Amino Acid Specific Stable Nitrogen Isotope Values In Avian Tissues: Insights From Captive American Kestrels And Wild Herring Gulls, C.E. Hebert, B.N. Popp, K.J. Fernie, C. Ka’Apu-Lyons, B.A. Rattner, N. Wallsgrove

United States Geological Survey: Staff Publications

Through laboratory and field studies, the utility of amino acid compound-specific nitrogen isotope analysis (AA-CSIA) in avian studies is investigated. Captive American kestrels (Falco sparverius) were fed an isotopically characterized diet and patterns in δ15N values of amino acids (AAs) were compared to those in their tissues (muscle and red blood cells) and food. Based upon nitrogen isotope discrimination between diet and kestrel tissues, AAs could mostly be categorized as source AAs (retaining baseline δ15N values) and trophic AAs (showing 15N enrichment). Trophic discrimination factors based upon the source (phenylalanine, Phe) and trophic (glutamic acid, Glu) AAs were 4.1 (muscle) …

Limited Antigenic Diversity In Contemporary H7 Avian-Origin Influenza A Viruses From North America, Yifei Xu, Elizabeth Bailey, Erica Spackman, Tao Li, Hui Wang, Li-Ping Long, John A. Baroch, Fred L. Cunningham, Xiaoxu Lin, Richard G. Jarman, Thomas J. Deliberto, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Subtype H7 avian–origin influenza A viruses (AIVs) have caused at least 500 confirmed human infections since 2003 and culling of >75 million birds in recent years. Here we antigenically and genetically characterized 93 AIV isolates from North America (85 from migratory waterfowl [1976–2010], 7 from domestic poultry [1971–2012], and 1 from a seal [1980]). The hemagglutinin gene of these H7 viruses are separated from those from Eurasia. Gradual accumulation of nucleotide and amino acid substitutions was observed in the hemagglutinin of H7 AIVs from waterfowl and domestic poultry. Genotype characterization suggested that H7 AIVs in wild birds form diverse and …

Pranked By Audubon: Constantine S. Rafinesque’S Description Of John James Audubon’S Imaginary Kentucky Mammals, Neal Woodman

United States Geological Survey: Staff Publications

The North American naturalist Constantine S. Rafinesque spent much of the year 1818 engaged in a solo journey down the Ohio River Valley to explore parts of what was then the western United States. Along the way, he visited a number of fellow naturalists, and he spent more than a week at the Henderson, Kentucky, home of artist and ornithologist John James Audubon. During the succeeding two years, Rafinesque published descriptions of new species that resulted from his expedition, including eleven species of fishes that eventually proved to have been invented by Audubon as a prank on the credulous naturalist. …

Transcriptional Profiling Of Ileocecal Valve Of Holstein Dairy Cows Infected With Mycobacterium Avium Subsp. Paratuberculosis, Randy J. Hempel, J. P. Bannantine, Judith R. Stabel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Johne’s disease is a chronic infection of the small intestine caused by Mycobacterium avium subspecies paratuberculosis (MAP), an intracellular bacterium. The events of pathogen survival within the host cell(s), chronic inflammation and the progression from asymptomatic subclinical stage to an advanced clinical stage of infection, are poorly understood. This study examines gene expression in the ileocecal valve (ICV) of Holstein dairy cows at different stages of MAP infection. The ICV is known to be a primary site of MAP colonization and pro-vides an ideal location to identify genes that are relevant to the progression of this disease. RNA was prepared …

Age-Related Hearing Loss: Inhibition--Excitation Balance In The Rat Inferior Colliculus, Sydney Cason 16

Honor Scholar Theses

Aging is an increasingly important research topic due to the aging populations in the United States and other developed countries. One aspect of aging, age-related hearing loss, is caused by changes in the peripheral and the central auditory systems. The age-related changes in the central auditory system, including the integrating midbrain area inferior colliculus (IC), are not well understood. Studies have revealed that GABA levels in the IC decrease with age. However, no studies have quantified the age-related changes in IC glutamate levels or the relationship between GABA and glutamate levels in the aging IC. This study aimed to elucidate …

Collective Action And Decision Making: An Analysis Of Economic Modeling And Environmental Free-Riding, Thomas Miller 16

Honor Scholar Theses

No abstract provided.

No Refuge: Hungarian Romani Refugee Claimants In Canada, Sean Rehaag, Julianna Beaudoin, Jennifer Danch

Osgoode Hall Law Journal

From 2008 to 2012, thousands of Hungarian Roma sought asylum in Canada. Some political actors suggested that their claims were unfounded and demonstrated that Canada’s refugee processes were vulnerable to abuse. In contrast, advocates for refugees argued that persecution against Roma was rampant in Hungary and noted that hundreds of Hungarian Roma were granted refugee status in Canada. Much of this debate has occurred in an evidentiary vacuum. This article fills this vacuum through a qualitative and quantitative study of Hungarian Romani refugee claims. First, the context of the study is discussed. Then, the article explores the experiences of Hungarian …

Islamic Law And Constitution-Making: The Authoritarian Temptation And The Arab Spring, Mohammad Fadel

Osgoode Hall Law Journal

In the wake of the Egyptian military coup of 3 July 2013, much commentary has focused on the religious-secular divide in Egypt as the principal division that laid the groundwork for the subsequent coup. Less attention has been paid to the profound divisions within religiously-minded Egyptian political actors regarding whether democratic or authoritarian government is more desirable from a religious perspective. This article explores the division between Islamist supporters of a “republican” conception of a modern Muslim constitutional and religious order, and Islamist supporters of an “authoritarian” conception of constitutional government in alliance with a state-supported religious establishment. The article …