Examination Of Larval Transport, Distribution And Hybridization Patterns Of Two Blue Mussel Species Mytilus Edulis And M. Trossulus In Machias Bay, Maine, Elizabeth A. Prochaska

All Theses And Dissertations

Using an interdisciplinary approach, this study examined the population connectivity of the blue mussel Mytilus trossulus within Machias Bay, Maine, near its inshore southern boundary range. The Eastern Maine Coast Current (EMCC) flows southwestward along the northern Gulf of Maine coastline, just outside of Machias Bay and is a potential pathway for M. trossulus larvae. This study compared results to two historical studies that assayed M. trossulus and M. edulis species distributions to evaluate any temporal changes. Both similarities and differences were found in species composition in Machias Bay. Historical data suggested that the lower Machias Bay was supplied by …

Identification Of Morphemes And The Effect Of Memory Load On Second Language Learning, James A. Hebert

Honors Capstones

No abstract provided.

Using Foraminifera Shell Geochemistry To Test Proxies For Paleoenvironmental Reconstruction, Catherine Hudson

Honors Capstones

No abstract provided.

The Effect Of Dietary Salt Intake On The Subcellular Distribution Of Epithelial Na+ Channels In Vasopressin And Oxytocin Synthesizing Neurons In The Rat Hypothalamus, Richard Frank Guidry

Honors Capstones

No abstract provided.

Atlantic Casualty Insurance Company V. Greytak: Adopting The Notice-Prejudice Rule And Clarifying Misinterpretations, Kristen Zadick

Montana Law Review Online

Seeking to clarify past decisions and protect insureds’ rights to insurance coverage, the Montana Supreme Court formally adopted the notice-prejudice rule in third party coverage disputes in Atlantic Casualty Insurance Company v. Greytak. However, in doing so, the Court failed to fully reconcile the decision with prior case law regarding the noticeprejudice issue.

Quantitative Analysis Of The Total Bacteria, Lactobacillus, And Bifidobacterium Colonic Microflora In Rats Fed Conventional, Prebiotic, And Probiotic Soy Diets, Deborah Osterholm Jung

Theses

Research suggests that specific compositions of gut microbiota can directly affect energy harvesting and fat storage, which may indicate a potential role of intestinal bacteria in the regulation of body weight (i.e., obesity). The purpose of the current study was to determine if prebiotic- and probiotic-based diets modify gut microbiota in genetically obese rodents. For this, female Zucker diabetic fatty (ZDF) rats were assigned diets containing fructooligosaccharides (FOS), Bifidobacterium (BIF), or Lactobacillus (LAC) for three weeks. qPCR was then used to measure levels of colonic Bifidobacterium, Lactobacillus, and total bacteria. At termination, there was no significant difference in Lactobacillus levels …

The Effect Of Conservation Tillage And Topographic Position On Soil Properties In Central Illinois, Andrew Mellinger

Theses

Since agriculture began, field management has been at the forefront of expanding food production beyond previous limitations. Agricultural productivity is closely related to the physical, chemical, and biological properties of the soil. Landscape position and field management are among primary factors affecting these soil properties. Delineation of topographic positions of the field surface by shape (i.e., convex, concave, and linear) characterizes areas that may accumulate or lose soil and nutrients either during a discrete event or cumulatively over several growing seasons. Increased soil compaction, degradation of soil structure, and erosion have all been attributed to declining agricultural production. In addition …

Developing Chemically Mutagenized Ems Forrest Soybean Population For High Oil Profile, Joshua William Gunther

Theses

AN ABSTRACT OF THE THESIS OF JOSHUA GUNTHER, for the Masters of Science degree in Plant, Soil and Agricultural Systems, presented on November 2nd, 2015, at Southern Illinois University Carbondale. TITLE: DEVELOPING CHEMICALLY MUTAGENIZED EMS FORREST SOYBEAN POPULATION FOR HIGH OIL PROFILE. MAJOR PROFESSOR: Dr. Khalid Meksem SUPERVISOR: Dr. Naoufal Lakhssassi Soybeans (Glycine max (L.) Merr.) are the most important crop that provides a sustainable source of oil and protein worldwide. Five major fatty acids are known, Palmetic, Stearic, Oleic, Linoleic and Linolenic acid, and each is essential for both nutrition and biodiesel. Oil demand for biodiesel production is constantly …

Understanding Chytridiomycosis Resistance By Investigating The Cutaneous Defense Mechanisms Of Marsupial Frogs, David Burkart

Theses

Anurans are declining worldwide because of the spread of Batachochytrium dendrobatidis (Bd), the fungus that causes chytridiomycosis. However, some frogs are resistant to this disease, and understanding why may be critical to saving those that are susceptible. In Peru, Gastrotheca excubitor is resistant to chytridiomycosis while Gastrotheca nebulanastes is susceptible. Two anuran skin defenses, symbiotic bacteria and antimicrobial peptides (AMPs), have demonstrated the ability to inhibit Bd in vitro when isolated from certain frogs. We tested if these defenses can explain the difference in susceptibility between the two Gastrotheca species. The cutaneous bacteria and AMPs of both species were collected, …

Jane Addams And Hans-Georg Gadamer: Learning To Listen With The Other, Mike Patrick Jostedt

Dissertations

This dissertation is an attempt to explain how listening functions in ethical and political contexts. I put forward a three-fold way of listening that begins in selfishness and ends in empathy. These three ways of listening I refer to as: “listening-for,” “listening-to,” and “listening-with.” I will briefly explain how each of these ways of listening function in lived experience. Listening-for is self-centered, listening-to deals in relations between self and other, and listening-with involves both parties (individuals or groups) working together. These forms of listening are implicitly situated in both Hans-Georg Gadamer and Jane Addams. The bulk of the dissertation is …

It’S All In How You Use It: Managers’ Use Of Meetings To Reduce Employee Intentions To Quit, Joseph E. Mroz, Joseph A. Allen

Psychology Faculty Publications

Meetings are often viewed as unnecessary, wastes of time, and overall negative experiences at work. In this study, we examined the positive side of meetings, specifically, how the relationship a manager fosters with subordinates in meetings affects those employees’ intentions to quit (ITQ). Using an online survey of working adults who regularly attended meetings, we found that the relation between perceived organizational support (POS) and leader–member exchange (LMX) quality in meetings on ITQ depended on an employee’s level of negative affectivity (NA). When POS or LMX in meetings was low or average, high-NA employees held significantly higher ITQ than low-NA …

Identifying Protein Kinase Tbk1 As A Novel Inhibitor Of Intestinal Tumorigenesis, Amber L. Mathews

Dissertations and Theses (Open Access)

Colorectal cancer (CRC) is the third most common cancer diagnosed in women and men， causing almost 600,000 annual deaths worldwide. There is a clear need to understand how CRC forms and progresses in order to improve the strategies of CRC prevention and therapy. A major factor that drives the development of CRC is genetic mutations that lead to activation of oncogenes and inactivation of tumor suppressor genes in intestinal epithelial cells (IECs). In addition, the initiation and progression of CRC involve environmental and immunological factors. In particular, chronic inflammatory conditions are known as an important risk factor for CRC. Intestinal …

The Role Of Dna Methylation And Hydroxymethylation In Smooth Muscle Cell Phenotypic Change During Atherogenesis, Jason Williams

Dissertations and Theses (Open Access)

Atherosclerosis is a chronic arterial disease which impacts systemically the function of organs and tissues by causing inflammation and disruption of blood supply. During atherogenesis, lipid deposits, inflammation, and cells accumulate within the walls of arteries, ultimately leading to a buildup of fatty plaques and occlusion of the arterial lumen. In a multifactorial disease like atherosclerosis there are complex interplays between environmental, genetic and epigenetic risk factors. The epigenetics of atherosclerosis involving DNA methylation has been progressing from single gene to genome wide analyses. Although these epistudies have provided evidence that DNA methylation exists within certain genes during atherogenesis, the …

Prediction Of Laser Ablation In Brain: Sensitivity, Calibration, And Validation, Samuel J. Fahrenholtz

Dissertations and Theses (Open Access)

The surgical planning of MR-guided laser induced thermal therapy (MRgLITT) stands to benefit from predictive computational modeling. The dearth of physical model parameter data leads to modeling uncertainty. This work implements a well-accepted framework with three key steps for model-building: model-parameter sensitivity analysis, model calibration, and model validation.

The sensitivity study is via generalized polynomial chaos (gPC) paired with a transient finite element (FEM) model. Uniform probability distribution functions (PDFs) capture the plausible range of values suggested by the literature for five model parameters. The five PDFs are input separately into the FEM model to gain a probabilistic sensitivity response …

Characterization Of Erbb4 Expression In Osteosarcoma, Nupur Lala

Dissertations and Theses (Open Access)

ERBB4 (Her-4) is a member of the ERBB family of Class I receptor tyrosine kinases. ERBB4 is unique within the ERBB family for alternate splicing in its juxtamembrane and cytoplasmic regions, which produces four juxtamembrane isoforms and two cytoplasmic isoforms. The cleavable juxtamembrane isoforms, Jm-a and Jm-d, can undergo proteolytic cleavage and produce an 80-kDa cytoplasmic fragment referred to as “p80,” which has been demonstrated to enhance cellular survival and malignant behavior in many solid tumors [44]. ERBB4 was almost exclusively 80 kDa in size and localized to the nucleus in primary tumors and metastases in studies characterizing ERBB4 expression …

Regulation Of The Oxidative Stress Response By Arid1a, Suet Yan Kwan

Dissertations and Theses (Open Access)

SWI/SNF is mutated in about 20% of all human cancers; in particular ARID1A is the most frequently mutated SWI/SNF subunit. ARID1A is a tumor suppressor gene, inactivating mutations in ARID1A are most frequently found in ovarian and endometrial cancers, specifically uterine corpus endometrioid carcinomas (UCEC), ovarian clear cell carcinomas (OCCC) and ovarian endometrioid carcinomas (OEC). The functional roles of ARID1A are not completely understood and there are limited therapeutic strategies that specifically target ARID1A-mutant cancers. Given that ARID1A expression is lost in cancer, ARID1A mutations cannot be targeted directly and novel therapeutic strategies are required to target ARID1A-mutant …

In Vivo Functional Significance Of Ccat2 Long Non-Coding Rna In Myelodysplastic Syndrome, Maitri Y. Shah

Dissertations and Theses (Open Access)

Long non-coding RNAs form the largest part of the mammalian non-coding transcriptome and control gene expression at various levels including chromatin modification, transcriptional and post-transcriptional processing. Although the underlying molecular mechanisms are not yet entirely understood, lncRNAs are implicated in initiation and progression of several cancers. CCAT2 is a lncRNA that spans the highly conserved 8q24 region associated with increased risk for various cancers. CCAT2 has been shown to play an important role in inducing chromosomal instability and supporting cell proliferation and cell cycle arrest. However, a causal role of CCAT2 in initiation of tumorigenesis and the importance of G/T …

Role Of Stat3 In Human Nk Cell Functions, Prasad V. Phatarpekar

Dissertations and Theses (Open Access)

Natural Killer (NK) cells are cytotoxic lymphocytes, which play a critical role in the immune response against malignant cells and microbial infections. NK cells are equipped with activating receptors, which upon detecting ligands expressed on stressed cells induce cytolytic activity of NK cells. Stimulation of NK cell proliferation and priming of NK cytolytic capability are accomplished by cytokines, which mediate their signals mainly through JAK-STAT signaling pathway. Previously, we found that K562 cells genetically modified to express membrane bound IL-21 (mbIL-21), which predominantly activates STAT3, induce robust expansion and activation of human NK cells. Further investigations revealed role of STAT3 …

Mobilization Of Adipose Stromal Cells In Obesity And Cancer By Sparc And Its Proteolytic Isoforms, Chieh Tseng

Dissertations and Theses (Open Access)

Obesity increases cancer risk and progression as shown by epidemiologic studies. However, the underlying pathophysiology remained unclear. Adipose stromal cells (ASC) are adipose tissue-derived mesenchymal progenitors, abundant in white adipose tissue (WAT). In this study, we show that the ASC pool is expanded in obesity and is associated with promoted tumor growth. Next, by using a chimeric GFP-RFP bone marrow transplant model, we observed higher tumor infiltrating cells with ASC phenotype in obese mice compared to lean. Consistently, systemic circulating ASC frequency is six fold higher in tumor-bearing obese mice compared to lean. The tumor infiltrating cells with ASC phenotype …

Determining The Mechanisms Generating Soluble Il-15 Complexes, Scott Anthony

Dissertations and Theses (Open Access)

A diverse assortment of infectious pathogens and TLR agonists enhance the expression of Interleukin (IL)-15. Additionally, inducing lymphopenia enhances anti-tumor responses in an IL-15-dependent manner. Paradoxically, despite the limited expression of IL-15 during homeostasis, the role of IL-15 during the steady state is well-known, while its roles during inflammation and infections remain largely undefined. IL-15 uses a unique method of production and presentation to support the development and homeostasis of NK and CD8 T cells. IL-15 is produced with its high affinity IL-15Rα and this IL-15Rα/IL-15 complex is shuttled to the cell surface where it is presented in-trans or cleaved …

The Tumor Suppressor Notch Inhibits Head And Neck Squamous Cell Carcinoma (Hnscc) Tumor Growth And Progression By Modulating Proto-Oncogenes Axl And Ctnnal1 (Α-Catulin), Shhyam Moorthy, Shhyam Moorthy

Dissertations and Theses (Open Access)

Background: Head and Neck Squamous Cell Carcinoma (HNSCC) is the sixth most common malignancy worldwide, with roughly 300,000 cancer related deaths occurring globally each year. The survival of patients with HNSCC has not changed significantly over the past decade, leading investigators to search for promising molecular targets. To identify new treatment targets and biomarkers that could better guide therapy, we previously characterized the genomic alterations from primary HNSCC patient samples. We were among the first to discover that NOTCH1 is one of the most frequently mutated genes in this cancer type. The spectrum of inactivating NOTCH1 mutations in HNSCC suggested …

Voxel-Level Absorbed Dose Calculations With A Deterministic Grid-Based Boltzmann Solver For Nuclear Medicine And The Clinical Value Of Voxel-Level Calculations, Justin Mikell

Dissertations and Theses (Open Access)

Voxel-level absorbed dose (VLAD) is rarely calculated for nuclear medicine (NM) procedures involving unsealed sources or 90Y microspheres (YM). The current standard of practice for absorbed dose calculations in NM utilizes MIRD S-values, which 1) assume a uniform distribution in organs, 2) do not use patient specific geometry, and 3) lack a tumor model. VLADs overcome these limitations. One reason VLADs are not routinely performed is the difficulty in obtaining accurate absorbed doses in a clinically acceptable time. The deterministic grid-based Boltzmann solver (GBBS) was recently applied to radiation oncology where it was reported as fast and accurate for both …

Normal Glycolytic Enzyme Activity Is Critical For Hypoxia Inducible Factor-1a Activity And Provides Novel Targets For Inhibiting Tumor Growth, Geoffrey Grandjean Phd

Dissertations and Theses (Open Access)

Normal Glycolytic Enzyme Activity is Critical for Hypoxia Inducible Factor-1α Activity and Provides Novel Targets for Inhibiting Tumor Growth

By Geoffrey Grandjean

Advisory Professor: Garth Powis, D. Phil

Unique to proliferating cancer cells is the observation that their increased need for energy is provided by a high rate of glycolysis followed by lactic acid fermentation in a process known as the Warburg Effect, a process many times less efficient than oxidative phosphorylation employed by normal cells to satisfy a similar energy demand ^[1]. This high rate of glycolysis occurs regardless of the concentration of oxygen in the cell and …

Redirecting T Cells With Chimeric Antigen Receptors To Target Cd123+ Leukemia, Radhika Thokala

Dissertations and Theses (Open Access)

ABSTRAC

REDIRECTING T CELLS WITH CHIMERIC ANTIGEN RECEPTORS TO TARGET CD123+ LEUKEMIA

Radhika Thokala, Ph.D*

Advisory Professor: Dean Anthony Lee, M.D, Ph.D

CD123 or interleukin receptor alpha (IL-3Rα) is expressed on hematological malignancies such as acute myeloid leukemia (AML) and some acute lymphoblastic leukemia (ALL). Significantly, CD123 is over-expressed on leukemic stem cells (LSCs) compared to normal hematopoietic stem cells and thus targeting this tumor- associated antigen (TAA) provides the potential to prevent relapse. The prototyical chimeric antigen receptor (CAR) is fashioned by …

Molecular Regulation Of Vascular Calcification In Murine Models Of Atherosclerosis, Shanshan Gao

Dissertations and Theses (Open Access)

Background: Calcification occurs often in the atherosclerotic lesions of patients with coronary heart disease and animals with hypercholesterolemia, such as apolipoprotein-E deficient (ApoE-/-) mice. However, the mechanism(s) underlying the development of calcification in atherosclerosis remains unclear. ApoE acts as a lipid transporter, but also has been recognized as a potential regulator of osteogenesis. Little information is available as to whether ApoE has any direct impact on osteogenesis and calcification in vascular smooth muscle cells (VSMC). Several signal transduction pathways play a role in regulation of calcification, including the Wnt/β-catenin system and potentially GTAP, an ubiquitin-conjugating enzyme responsible for protein …

Effects Of Pharmacological And Genetic Disruption Of Chemokine Receptor Cxcr4 In B-Cell Acute Lymphoblastic Leukemia, Shubhchintan Randhawa

Dissertations and Theses (Open Access)

The CXCR4 chemokine receptor plays a pivotal role in homing and retention of B-cell Acute Lymphoblastic Leukemia cells to bone marrow niches. The bone marrow stromal cells (BMSCs) constitutively secrete the CXCR4 ligand CXCL12, thus attracting leukemia cells and protecting them from cytotoxic drugs, a mechanism that might be responsible for minimal residual disease after conventional chemotherapy against B-ALL. Indeed, in this study we observed that B-ALL cells that had migrated beneath the BMSCs were highly resistant to chemotherapeutic drugs. Inhibition of CXCR4 to disrupt this interaction presents itself as an attractive target to overcome bone marrow derived drug resistance …

Defining The Molecular Networks Necessary For Thymus Fate And Organogenesis, Kaitlin A. Reeh

Dissertations and Theses (Open Access)

The thymus and parathyroid (PT) glands originate from endodermal progenitors in the bilateral third pharyngeal pouches (3^rd pps). By E11.5 during mouse development, cells committed to the thymus lineage express Foxn1 whereas PT-fated cells express Gcm2. While these transcription factors are required for organ-specific differentiation, the exact molecular mechanisms that specify endodermal progenitors to either the thymus or parathyroid lineage are not well defined. Tbx1 is initially expressed throughout the 3^rd pp endoderm, as it is required for segmentation of the pharyngeal apparatus, but is downregulated in the thymus-fated domain by E10.5. Despite the widely held notion …

An Automated Tool For High Resolution Visualization Applied To Transient Watershed Models, Noah Robert Taylor

Theses and Dissertations

Numeric hydrologic models can aid in water resource management by providing predictive simulations of water behavior. As computers become more advanced, the models developed also become more complex using more data to represent larger areas for forecasting hydrologic behavior. Unfortunately, as the simulations use more data, the output often becomes difficult to manage and share without investing time and effort into setting up server environments or decreasing the quality of the output to compensate for an efficient and effective user experience. A proposed solution to facilitate the accessibility of massive hydrologic model output is through the web-based visualization tool developed …

Compression Bodies And Their Boundary Hyperbolic Structures, Vinh Xuan Dang

Theses and Dissertations

We study hyperbolic structures on the compression body C with genus 2 positive boundary and genus 1 negative boundary. We consider individual hyperbolic structures as well as special regions in the space of all such hyperbolic structures. We use some properties of the boundary hyperbolic structures on C to establish an interesting property of cusp shapes of tunnel number one manifolds. This extends a result of Nimershiem in [26] to the class of tunnel number one manifolds. We also establish convergence results on the geometry of compression bodies. This extends the work of Ito in [13] from the punctured-torus case …

The Role Of Documentary Film In The Emerging Social Entrepreneurial Culture, Karyn Elizabeth Daley

Theses and Dissertations

Considering the current skepticism surrounding the impact and efficacy of nonprofit and nongovernmental organizations, some believe that a unique category of innovator known as the social entrepreneur may be society’s best hope for bringing innovative, scalable, and systemic solutions to bear on the world’s most intractable problems. Social entrepreneurs, as defined by Ashoka, have a unique set of characteristics that determine not only how they move within the world of social change-making but also how they communicate their ideas and mission to the public.

This exploratory study reviewed how social entrepreneurs currently use documentary film and visual media in their …