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Graduate Bulletin | 2015-2016, Jacksonville State University

Bulletins & Catalogs | Graduate

Vol. 37, No. 1 (August 2015). Jacksonville State was granted University status in 1966 and began adding graduate programs to its curriculum at that time. Graduate bulletins and catalogs contain information on the academic calendar, policies and procedures, faculty, scholarships and tuition, and degree requirements for graduate programs.

Establishment Of Immortalized Innate Lymphoid Cell Lines From The Mouse Lung, Celine A. Beamer

University Grant Program Reports

The purpose of this proposal was to create immortalized innate lymphoid cell (ILC) lines from normal and transgenic mice

Versatility Of Using Major Histocompatibility Complex Class Ii Dextramers For Derivation And Characterization Of Antigen-Specific, Autoreactive T Cell Hybridomas, Bharathi Krishnan, Chandirasegaran Massilamany, Rakesh H. Basavalingappa, Rajkumar A. Rajasekaran, Charles Kuszynski, Barbara Switzer, Daniel A. Peterson, Jay Reddy

Jay Reddy Publications

Antigen-specific, T cell hybridomas are useful to study the cellular, molecular and functional events, but their generation is a lengthy process. Thus, there is a need to develop robust methods to generate the hybridoma clones rapidly in a short period of time. To this end, we have demonstrated a novel approach using major histocompatibility complex (MHC) class II dextramers to generate T cell hybridomas for an autoantigen, proteolipid protein (PLP) 139–151. Using MHC class II dextramers assembled with PLP 139–151 as screening and sorting tools, we successfully obtained mono antigen-specific clones within seven to eight weeks. In conjunction with other …

Mirtarvis: An Interactive Visual Analysis Tool For Microrna-Mrna Expression Profile Data, Daekyoung Jung, Bohyoung Kim, Robert J. Freishtat, Manta Giri, Eric P. Hoffman, Jinwook Seo

Pediatrics Faculty Publications

Background

MicroRNAs (miRNA) are short nucleotides that down-regulate its target genes. Various miRNA target prediction algorithms have used sequence complementarity between miRNA and its targets. Recently, other algorithms tried to improve sequence-based miRNA target prediction by exploiting miRNA-mRNA expression profile data. Some web-based tools are also introduced to help researchers predict targets of miRNAs from miRNA-mRNA expression profile data. A demand for a miRNA-mRNA visual analysis tool that features novel miRNA prediction algorithms and more interactive visualization techniques exists.

Results

We designed and implemented miRTarVis, which is an interactive visual analysis tool that predicts targets of miRNAs from miRNA-mRNA …

Bioregulatory Systems Medicine: An Innovative Approach To Integrating The Science Of Molecular Networks, Inflammation, And Systems Biology With The Patient's Autoregulatory Capacity?, Alyssa W Goldman, Yvonne Burmeister, Konstantin Cesnulevicius, Martha Herbert, Mary Kane, David Lescheid, Timothy Mccaffrey, Myron Schultz, Bernd Seilheimer, Alta Smit, Georges St Laurent, Brian Berman

Medicine Faculty Publications

Bioregulatory systems medicine (BrSM) is a paradigm that aims to advance current medical practices. The basic scientific and clinical tenets of this approach embrace an interconnected picture of human health, supported largely by recent advances in systems biology and genomics, and focus on the implications of multi-scale interconnectivity for improving therapeutic approaches to disease. This article introduces the formal incorporation of these scientific and clinical elements into a cohesive theoretical model of the BrSM approach. The authors review this integrated body of knowledge and discuss how the emergent conceptual model offers the medical field a new avenue for extending the …

Mantle Cell Lymphoma: Current Concepts, Manish K. Pant, Weaam Alshenawy, Ahmed Alrajjal, Hussain Alrobeh, Imad A. Tabbara

Medicine Faculty Publications

Mantle Cell Lymphoma is a rare B-cell malignancy that can invade almost any structure in the body and recur after short-lived clinical responses. The pathogenesis and clinical features are well defined, but management has not yet been optimized. Induction with traditional immune-chemotherapy regimens that are used in other nonHodgkin lymphomas rarely generate durable remissions. Therefore, clinical research is needed to improve treatment of de novo disease and to establish safe and effective regimens for maintenance and salvage options for relapsed or refractory disease. This comprehensive review discusses disease pathogenesis and focuses on emerging treatment paradigms using novel targeted therapies.

Relationships Between Growth Rate And Gene Expression In Ruegeria Pomeroyi Dss-3, A Model Marine Alphaproteobacterium, Nattasha Vinas

All Theses

Microbes are important contributors to ecosystem processes such as biogeochemical cycling. Their activities vary, depending on environmental parameters such as carbon type and concentration, temperature, and salinity. Current estimates of in situ microbial growth is limited to gross estimates or rely on incubation based methods that disturb the natural state of the community. The goal of this master’s thesis is to develop molecular methods to directly assess microbial growth rates in the environment at the level of a taxonomic group. Here we grew Ruegeria pomeroyi DSS-3 under different temperatures (15 °C or 30 °C) and different carbon sources (yeast extract/tryptone, …

Epithelial And Fibrous Hyperplasia: An Oral Manifestation Of Tuberous Sclerosis Complex., Sandra U. Mbibi Dds, Stuart L. Segelnick Dds, Mea A. Weinberg Dmd

Individual Scholarship

The authors present a case study of a 13-year-old female with a past medical history of tuberous sclerosis complex (TSC), an autosomal dominant disorder. It usually presents with a triad of epilepsy, mental deficiency and facial angiofibromas that are often dis tributed around the nose, cheek and chin, and are frequently shaped like butterfly wings. In addition, oral manifestations include gingival enlargement and developmental enamel pitting on the facial aspect of the anterior permanent dentition in 50% to 100% of patients. The patient’s chief complaint was gingival enlargement and gingival bleeding. The histology of the excised gingival tissue revealed epithelial …

Optimisations And Challenges Involved In The Creation Of Various Bioluminescent And Fluorescent Influenza A Virus Strains For In Vitro And In Vivo Applications, Monique I. Spronken, Kristy Short, Sander Herfst, Theo M. Bestebroer, Vincent P. Vaes, Erin Sorrell, + 9 More

Health Policy and Management Faculty Publications

Bioluminescent and fluorescent influenza A viruses offer new opportunities to study influenza virus replication, tropism and pathogenesis. To date, several influenza A reporter viruses have been described. These strategies typically focused on a single reporter gene (either bioluminescent or fluorescent) in a single virus backbone. However, whilst bioluminescence is suited to in vivo imaging, fluorescent viruses are more appropriate for microscopy. Therefore, the idea l reporter virus varies depending on the experiment in question, and it is important that any reporter virus strategy can be adapted accordingly. Herein, a strategy was developed to create five different reporter viruses in a …

Gender And Climate Change In The Indian Himalayas: Global Threats, Local Vulnerabilities, And Livelihood Diversification At The Nanda Devi Biosphere Reserve, Monica V. Ogra, Ruchi Badola

Environmental Studies Faculty Publications

Global climate change has numerous implications for members of mountain communities who feel the impacts in both physical and social dimensions. In the western Himalayas of India, a majority of residents maintain a livelihood strategy that includes a combination of subsistence or small-scale agriculture, livestock rearing, seasonal or long-term migration, and localized natural resource extraction. While warming temperatures, irregular patterns of precipitation and snowmelt, and changing biological systems present challenges to the viability of these traditional livelihood portfolios in general, we find that climate change is also undermining local communities’ livelihood assets in gender-specific ways. In this paper, we present …

Sustaining Excellence, University Of Dayton. Advancement Relations

Donor impact reports

2014 Impact Report from the University of Dayton.

August 2015 Commencement Program, Bowling Green State University

Commencement Programs

No abstract provided.

Biological Sciences Newsletter - August 2015, Department Of Biological Sciences, East Tennessee State University

Biological Sciences Newsletters

No abstract provided.

2015-2016 Faculty Senate Roster, Faculty Senate, East Tennessee State University

Faculty Senate Agendas and Minutes

No abstract provided.

Utah State Magazine, Fall 2015, Utah State University

Utah State Magazine

The quarterly magazine for friends and alumni of Utah State University.

Ribosome Component Turnover Kinetics Describes A Two-Pool Kinetic Model In Dietary Restriction That Suggests Rpl10 Is Exchanged During Ribosome Lifespan, Andrew David Mathis

Theses and Dissertations

The eukaryotic ribosome is a large molecular machine consisting of ~80 ribosomal proteins and 4 rRNAs. The 40S and 60S ribosomal subunits are assembled in the nucleolus by ~200 helper proteins then shipped into the cytoplasm or to the endoplasmic reticulum where protein translation takes place. Eventually ribosomes are removed from the cytoplasm and recycled through ribophagy, however, very is little is known about ribosomal protein exchange after assembly but before ribophagy. Using kinetic turnover measurements of ribosomal proteins and rRNA in vivo we determined ribosomal protein replacement rates are diverse suggesting ribosomal components may be replaced without destruction of …

An Investigation Of The Mechanisms Promoting Toxic Dinoflagellate Blooms In Estuarine Ecosystems, Theresa K. Hattenrath

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Dinoflagellates can cause harmful algal blooms (HABs) associated with human health syndromes such as paralytic shellfish poisoning (PSP) and diarrhetic shellfish poisoning (DSP) and these HABs are a growing concern in many coastal regions. My dissertation explored the factors promoting toxic dinoflagellate blooms in estuarine ecosystems. Northport Bay, NY, which has experienced chronic PSP-induced shellfish closures due to the toxic dinoflagellate Alexandrium fundyense and blooms of the DSP-producer, Dinophysis acuminata, was used as a natural system to explore these factors while multiple strains of A. fundyense and a newly established D. acuminata culture isolated from North America were investigated in …

Material Characterization Of Healthy And Osteoporotic Rat Femur Bone, Kaushik Chava

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Bone is a bio composite material made of organic and inorganic components. This unique material composition supported by hierarchical level structure enables it to perform wide range of activities and functions in human body. The inorganic phase consists of hydroxyapatite mineral crystals and the majority of organic phase is made of collagen fibers. A mineralized collagen fiber is the basic building unit in the structure of bone. The ability of bone to fine-tune its structure according to mechanical stress and repair its damaged regions differentiates it from modern synthetic materials. The bone modelling and remodeling is a very complex process …

Effects Of Aligned Fiber Substrates And P12 Peptide On Cell Migration, Kao Li

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Cell migration is the critical process in the development and maintenance of multicellular organisms. It's a highly integrated multistep process that orchestrates a series of cellular behavior, such as embryonic development, wound healing and immune responses. A lot of effort has been dedicated to studying the basic principle and mechanism of cell movement since decades ago1-3. Cells can migrate in single cell or en masse fashion. We have already known that En masse cell migration is more related with physiological processes of tissue formation. And previous study has shown that cells would respond to different substrates, with various morphology or …

Organic/Biological Hybrid Nanomaterials For Biosensing And Energy Conversion: From Fundamentals To Applications, Zhongwei Liu

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Water-soluble conjugated polymers have emerged as cost-effective, light harvesting materials for the development of highly sensitive and selective biosensing platforms where the detection of a target is recognized through changes in the spectroscopic properties of the conjugated polymer. My thesis describes fundamental studies of interaction in water-soluble conjugated polymers/ DNA organic/biological hybrids and the application of such hybrids in DNA sensing and energy conversion. In particular, I demonstrated a conjugated poly(phenylene vinylene (PPV)/DNA hybrid with sequence dependent photoluminescence and chiroptical properties, for which the PPV photoluminescence was found to be enhanced as high as seven fold upon binding with DNA. …

Novel Computational Methodology For Detecting And Quantifying Alternative Splicing From Rna-Seq Data, Jie Wu

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Recent development of ultra-high-throughput sequencing of the transcriptome (mRNA-Seq) provides a means of profiling RNA splicing events at unprecedented depth. On the other hand, the ultra-high coverage and the complexity brought by mRNA-Seq data also create big challenges for computational analysis. My Ph.D. work focuses on developing algorithms to detect, quantify and characterize alternative splicing (AS) from mRNA-Seq data. These algorithms include: (1) OLego, a fast and sensitive splice mapping program for mRNA-Seq data. The most important features of OLego include strategic and efficient searches with very small seeds (12~14 nt), and a built-in regression model to score exon junctions. …

Review Of Glucose Metabolism Dysregulation In Cancer, Moises Ovidio Guardado

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

It has been known for over 60 years that tumor metabolism is strikingly different than normal tissue metabolism. In this thesis, I review literature pertinent to cancer metabolism and how the altered regulation of metabolism helps tumors to meet their metabolic needs. Otto Warburg's pioneering studies on tumors first demonstrated that tumors rely on glycolysis even in the presence of oxygen. Warburg proposed that the high glycolytic rate in tumors was due to cancer cells' inability to perform mitochondrial oxidative phosphorylation (OXPHOS). Recent investigations do not support the idea of dysfunctional OXPHOS in cancer cells A new understanding of Warburs'g …

Characterization Of Disease Related Missense Mutations In Vacuolar Sorting Protein 13 Of Saccharomyces Cerevisiae, Robert Anthony Policastro

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Vps13 is a large evolutionarily conserved protein of 3144 amino acids found in S. cerevisiae. Vps13 has been connected to disparate roles in the cell, including the retention of proteins in the trans golgi network, proper phospholipid composition and closure of the prospore membrane during sporulation, and a genetic interaction with the ERMES complex which brings the ER and mitochondria to close proximity. Mutations in VPS13A, a VPS13 ortholog found in humans, has been linked to a late onset neurodegenerative disease similar to Huntington's disease, called chorea acanthocytosis (ChAc). Previous sequencing of VPS13A in some ChAc patients uncovered missense mutations, …

A Structural And Energetic Model For The Slow-Onset Inhibition, Cheng-Tsung Lai

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Slow-onset inhibitors are of particular interest in drug discovery programs as the slow dissociation of the inhibitor from the target-inhibitor complex prolongs target occupancy and improves in vivo efficacy. While slow-onset inhibition is observed in many enzymes and the kinetic equations used to describe slow-onset inhibition were derived more than 26 years ago, the structural basis for slow-onset inhibition is still not generally well understood, hindering prediction and control of slow-onset binding kinetics. An enzyme system with known experimental kinetics and structural data for multiple classic and slow-onset inhibition complexes would be an ideal model system for the study of …

Structural Changes In The Beta-Amyloid Precursor Protein Induced By Familial Mutations, Tzuchun Tang

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

The proteolysis of the beta-amyloid precursor protein (APP) yields Abeta peptides with lengths of 38-42 amino acids by a two-step sequential cleavage involving beta- and gamma-secretase. Abeta40 is the most abundant product, while Abeta42 is the most toxic species to neurons. An increase in the ratio of Abeta42/Abeta40 and an overall increase in secreted Abeta peptides have both been correlated with early-onset familial Alzheimer's disease (FAD). I have focused on two sites where FAD mutations are clustered. The first cluster is in the extracellular domain close to the transmembrane boundary. Within the clusters, the A21G (Flemish), E22Q (Dutch), E22G (Arctic), …

Nutritional Ecology And Growth In Juvenile Phayre's Leaf Monkeys, Kerry Michele Ossi-Lupo

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Primates exhibit slow growth and an extended juvenile period relative to other similar-sized mammals. Certain evolutionary explanations have linked the costs or benefits of bigger primate brains to slow life histories in general, although a link specifically to the length of juvenility is less clear. The ecological risk aversion hypothesis singles out the juvenile period as a target of selection, positing that slower growth rates and smaller size provide juveniles with an energetic buffer (i.e. | lower absolute metabolic demand) against starvation risk during a critical period of heightened vulnerability. This greater vulnerability is attributed largely to juveniles' lower foraging …

Modulation Of Rodent Central Respiratory Network Dynamics And Respiratory Phase Interactions, Tabitha Shen

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Modulation of the central neural control system governing breathing is a critical mechanism for maintaining homeostasis of a functioning physiological system. Perturbations can be either endogenous or exogenous to the respiratory control system, meaning there are factors that modulate the respiratory control centers either directly (endogenously) or indirectly via feedback mechanisms (exogenously). We hypothesized that perturbations to the neural controller would cause changes in the configuration of the neural network, as evidenced by changes in slow and fast oscillatory respiratory network dynamics. To test this hypothesis, we use both an in vitro and an in vivo rat experimental preparation in …

Major Depressive Disorder Is Related To A Broad Disruption In Brain Regions Underlying Working Memory Processing, John A. Borghi

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Major depressive disorder (MDD) is associated with significant deficits in information processing (Clark et al. | 2009). Previous studies examining the neural basis of these deficits have often focused on disruptions localized to a small number of brain regions or have used study paradigms that do not allow for an examination of component cognitive processes such as the selection and maintenance of items held in working memory (WM). Using a task previously employed to investigate selective information processing in healthy subjects (Oh & Leung, 2010), the experiment described in this thesis examined two questions regarding the effect of MDD on …

Synthesis Of A Fluorescent Sensor For Calcium Measurement In Marine Sediments, Jingyu Huang

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Ocean acidification has been shown to endanger shelled marine organisms, since increased CO₂ intake by oceans will decrease pH of seawater and enhance Ca2+ dissolution from shell skeletons. Determination of Ca2+ concentration in the marine environment would not only help to provide information about local calcium ion distribution and activities, but also provide a sketch of the living condition of shelled marine organisms. A number of optical sensors for calcium ion detection have been developed. However, these detectors were mostly designed for intracellular detections or relatively low ion concentration conditions (10− 6 to 10− 8 M) and have high …

Tungstate Sorption Mechanisms On Various Mineral Surfaces: Systematic Batch Uptake Experiments And Spectroscopic Studies, Hyuck Hur

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

The importance of this study is related to releases of tungsten to environment as a result of its widespread used in industrial, civil, and military applications. Recent studies have reported the oxidative dissolution of tungsten metal or alloy under appropriate pH and redox conditions resulting in the formation of tungstate, W(VI), which occurs mainly as oxyanions--some of which have a high solubility in aquatic and soil systems. Therefore, the environmental fate of W ultimately depends on an understanding of tungstate geochemistry. We will discuss the role of tungstate speciation on its sorption mechanism on environmentally relevant mineral surfaces, including aluminum …