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2015

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Using The Intact Method To Study Pickle In Individual Cell Types, Jacqueline L. Phipps, Daniela N. Martir, Ben Carter, Joe Ogas Aug 2015

Using The Intact Method To Study Pickle In Individual Cell Types, Jacqueline L. Phipps, Daniela N. Martir, Ben Carter, Joe Ogas

The Summer Undergraduate Research Fellowship (SURF) Symposium

Cell differentiation is an essential part of development in multicellular organisms. Cells with identical genomic DNA are able to differentiate into a variety of tissues due to selective expression and repression of genes. This tissue-specific gene expression is enabled in part by proteins called chromatin remodelers, which can move, remove, or restructure histone proteins to restrict or allow physical access to genomic DNA. PICKLE (PKL) is a member of the CHD family of ATP-dependent chromatin remodelers that promotes cellular identity in the plant model organism Arabidopsis thaliana. PKL promotes cell identity by silencing embryonic genes during seed germination by promoting …

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Selective Protein Labelling To Visualize Cellular Differentiation, Andrew J. Witten, Tamara L. Kinzer-Ursem Aug 2015

Selective Protein Labelling To Visualize Cellular Differentiation, Andrew J. Witten, Tamara L. Kinzer-Ursem

The Summer Undergraduate Research Fellowship (SURF) Symposium

Protein post-translational modifications serve to give proteins new cellular function, spatial localization, or enzymatic activity. Myristoylation is a common post-translational modification where the enzyme N-myristoyltransferase adds myristic acid onto the N-terminus of a variety of proteins. In this work we use a myristic acid analog, 12-azidododecanoic acid (12ADA) to facilitate the implementation of azide-alkyne cycloaddition reactions on myristoylated proteins. Selective protein labeling methods such as these are useful in research because they can be used to help determine the biological function of this protein lipid modification and can be extended to study disregulated protein myristoylation in disease states. To validate …

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Characterizing The Role Of Aif4 In Saccharomyces Cerevisiae, Antonia L. Hur Ms., Nina Serratore Ms., Scott D. Briggs Aug 2015

Characterizing The Role Of Aif4 In Saccharomyces Cerevisiae, Antonia L. Hur Ms., Nina Serratore Ms., Scott D. Briggs

The Summer Undergraduate Research Fellowship (SURF) Symposium

Chromatin remodelers are important regulatory mechanisms that eukaryotic cells use to modify the structure of chromatin, which is made up of DNA and proteins. DNA wraps around histone proteins to make up chromatin. When these proteins are modified, the shape of the chromatin is altered. “Loosening” the chromatin structure by chromatin modifications allows for active gene expression whereas “tightening” or compaction of chromatin results in gene repression. Therefore the modifications on chromatin modulate gene expression in all eukaryotes. It has been shown that mis-regulation of chromatin remodelers contribute to various cancers. Understanding the biochemistry behind how chromatin associating proteins modify …

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Design Of Transgenic S. Cerevisiae For Enzymatic Pretreatment, Mark Aronson, Leyla Yamin, Soo Jung Ha, Jenna Rickus, Michael E. Scharf Aug 2015

Design Of Transgenic S. Cerevisiae For Enzymatic Pretreatment, Mark Aronson, Leyla Yamin, Soo Jung Ha, Jenna Rickus, Michael E. Scharf

The Summer Undergraduate Research Fellowship (SURF) Symposium

Biofuels, combustible fuel produced from fermentation of agricultural biomass by microorganisms, represent one of the best possible paths forward for sustainable energy production. However, inefficiencies in biofuel production create barriers that stand in the way of their widespread adoption. One such barrier is the breakdown of lignin, a biopolymer that exists on the edge of plant cell walls which protects the sugars that are used in fermentation. Currently, lignin is broken down in energy-intensive thermal pretreatment processes. A viable alternative is the expression of lignin-degrading enzymes by synthetic microorganisms that work at standard temperatures, eliminating the need for the high-energy …

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Proteoglycan Mimic Of The Glycocalyx To Treat Endothelial Dysfunction, Victoria Messerschmidt, Alyssa Panitch, James R. Wodicka Aug 2015

Proteoglycan Mimic Of The Glycocalyx To Treat Endothelial Dysfunction, Victoria Messerschmidt, Alyssa Panitch, James R. Wodicka

The Summer Undergraduate Research Fellowship (SURF) Symposium

Patients with kidney failure usually undergo hemodialysis, a process by which toxins produced by the body are filtered from the blood, in order to survive. The preferred form for vascular access is called an arteriovenousfistula (AVF), a surgically created connection between an artery and vein that is utilized to undergo dialysis. However, AVFs have a failure rate of 50-60%. One of the contributions to AVF failure is endothelial cell dysfunction and loss of glycocalyx, which allows neutrophils and other native cells into the media of the vessel, which causes an inflammatory response. Our lab addresses endothelial dysfunction by mimicking the …

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Development Of A Novel Enzymatic Pre-Treatment For Lignocellulosic Biomass, Melissa Robins, Jenna Rickus Aug 2015

Development Of A Novel Enzymatic Pre-Treatment For Lignocellulosic Biomass, Melissa Robins, Jenna Rickus

The Summer Undergraduate Research Fellowship (SURF) Symposium

Biofuels, fuels derived directly from living matter, present a renewable and environmentally friendly alternative to petroleum based fuels. Bioethanol produced from low input energy crops or agricultural waste is a promising fuel source because it does not interfere with the human food supply chain and the ethanol produced can be blended with gasoline. These potential sources of bioethanol are not yet commercially viable due to a polymer called lignin present in the plant’s cell wall which impedes the conversion of cellulose to glucose and the eventual fermentation of glucose to ethanol. Developing new methods for the pretreatment of lignocellulosic biomass …

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Behavioral Outputs Of Fragile-X Autistic Mice Exposed To Open-Field, Randomized, Short-Term Visual Stimuli, Nicholas M. Dicola, Alexander A. Chubykin Aug 2015

Behavioral Outputs Of Fragile-X Autistic Mice Exposed To Open-Field, Randomized, Short-Term Visual Stimuli, Nicholas M. Dicola, Alexander A. Chubykin

The Summer Undergraduate Research Fellowship (SURF) Symposium

Animal models of different neurological disorders are required for studying the pathophysiology of these diseases, and for potential development of pharmacological and behavioral treatments. The scientific community often uses mouse models for behavior studies due to their powerful genetic tools and low cost. However, subjective measurement techniques are often used when analyzing mice for behavioral traits which often results in discrepancies in results. An automated tracking software would aim to eliminate these discrepancies and subjective analysis. This lab has developed a software program which offered an ability to automatically collect open-field behavioral data with simultaneous on-line analysis. Python, an open …

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Application Of Ultrasound In The Measurement Of Lubricant Fluid Film Thickness In The Piston-Cylinder Interface Of An Axial Piston Pump, Dhruv Subramaniam, Dan Mizell, Monika Ivantysynova Aug 2015

Application Of Ultrasound In The Measurement Of Lubricant Fluid Film Thickness In The Piston-Cylinder Interface Of An Axial Piston Pump, Dhruv Subramaniam, Dan Mizell, Monika Ivantysynova

The Summer Undergraduate Research Fellowship (SURF) Symposium

In this paper a feasibility study has been conducted to determine a technique for the measurement of lubricant fluid film thickness in the piston-cylinder interface of an axial piston pump. The thickness of the lubricant film has a significant impact on the efficiency of the piston pump yet it still remains an uncertainty. If the lubricant film is too thin then friction will cause excessive damage resulting in deformation of interacting surfaces resulting in further uncertainty in thickness measurement. If the lubricant film is too thick then there is excessive leakage which will compromise the efficiency of the system. Acoustic, …

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Ultrasound-Guided Nanobubbles For Targeted Drug Delivery, Pei Yang, Pushpak Bhandari, Joseph Irudayaraj Aug 2015

Ultrasound-Guided Nanobubbles For Targeted Drug Delivery, Pei Yang, Pushpak Bhandari, Joseph Irudayaraj

The Summer Undergraduate Research Fellowship (SURF) Symposium

In a large number of biological and environmental applications, ultrasound (US)-powered micro- and nano-motors have attracted considerable attention. However, their applications in biological settings have been limited due to the incompatibility of metallic motors and the lack of precision guiding. Here, we demonstrate that cellulosic polymer nanobubbles (200-800nm) can be propelled, aligned, accelerated, and assembled in solution using Doppler ultrasound beam (DUB) and simultaneously imaged using low-frequency ultrasound. Results show that by utilizing Doppler ultrasound beam (DUB), nanobubbles accumulation at a pre-determined site can be enhanced. Moreover, bubbles’ trajectory and velocity can be also be manipulated. Related parameters associated with …

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Buckling-Driven Force Generation Of Cell Cortex, Pranith Lomada, Wonyeong Jung, Taeyoon Kim Aug 2015

Buckling-Driven Force Generation Of Cell Cortex, Pranith Lomada, Wonyeong Jung, Taeyoon Kim

The Summer Undergraduate Research Fellowship (SURF) Symposium

Actomyosin cortex, a thin network underlying cell membrane, is known to generate a large portion of tensile forces required for various cellular processes. Recently, theoretical studies predicted that buckling of actin filaments breaks symmetry between tensile and compressive forces developed by myosin motors, resulting in tensile stress at a network level. However, the significance of the filament buckling of the cortex has yet to be demonstrated either computationally or experimentally. Here, buckling-dependent stress generation of the cortex-like actomyosin network was investigated using an agent-based computational model consisting of actin filaments, actin cross-linking proteins (ACPs), and molecular motors. First, a wide …

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Harnessing Notch Signaling For Biomaterial Scaffold-Based Bone Regeneration, Helena P. Lysandrou, Chunhui Jiang, Naagarajan Narayanan, Shihuan Kuang, Meng Deng Aug 2015

Harnessing Notch Signaling For Biomaterial Scaffold-Based Bone Regeneration, Helena P. Lysandrou, Chunhui Jiang, Naagarajan Narayanan, Shihuan Kuang, Meng Deng

The Summer Undergraduate Research Fellowship (SURF) Symposium

Bone fracture has recently become prevalent, especially with an increasingly aging population. Current bone grafts procedures, including autografts and allografts, are hindered by multiple factors, such as limited supplies and inconsistent bone healing. Scaffold-based bone tissue engineering emerges as a prospective strategy to aid in bone regeneration through delivery of growth factors such as bone morphogenic proteins (BMPs). However, the use of BMPs suffers from several drawbacks such as protein instability and immunogenicity. Therefore, there exists a great need for the development of novel therapies to promote bone healing. Notch signaling, a pathway critical for cell-fate determination has been shown …

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Test Rig Design For Compact Variable Displacement Vane Pump, Pratik Chawla, Ryan Jenkins, Monika Ivantysynova Aug 2015

Test Rig Design For Compact Variable Displacement Vane Pump, Pratik Chawla, Ryan Jenkins, Monika Ivantysynova

The Summer Undergraduate Research Fellowship (SURF) Symposium

Variable displacement vane pumps (VDVP) are one type of positive displacement pumps used in automatic transmission vehicles for lubricating the gears, cooling the transmission and actuating the clutches. Though fixed displacement pumps are widely used, they output a constant effective flow at a given speed. Depending on pump sizing considerations, the pump can be oversized at high speeds because flow demand of the transmission is independent of engine speed. The excess flow returns to the tank through an orifice that causes the oil to heat up, increasing the energy required for cooling and reducing the efficiency of the transmission. A …

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Captive Breeding Protocols And Their Impact On Genetic Diversity In White-Footed Mice (Peromyscus Leucopus): Implications For Threatened And Endangered Species, Maureen C. Lamb, Janna R. Willoughby, J. Andrew Dewoody Aug 2015

Captive Breeding Protocols And Their Impact On Genetic Diversity In White-Footed Mice (Peromyscus Leucopus): Implications For Threatened And Endangered Species, Maureen C. Lamb, Janna R. Willoughby, J. Andrew Dewoody

The Summer Undergraduate Research Fellowship (SURF) Symposium

Captive breeding protocols used in zoos often are aimed at increasing population sizes and retaining genetic diversity of endangered species. However, captive breeding causes genetic adaptation to captivity that can lead to an overall decrease in genetic diversity and reduce chances of a successful reintroduction to the wild. In this study, we assess how 3 different breeding protocols—random mating, preferential breeding of individuals with the lowest mean kinship scores, and selection for docility—affect the variability of mitochondrial DNA in white-footed mice (Peromyscus leucopus). We used mice that were captured from the wild but were mated for up to …

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Draft Genome Sequence Of Frankia Sp. Strain Dc12, An Atypical, Noninfective, Ineffective Isolate From Datisca Cannabina, Louis S. Tisa, Nicholas Beauchemin, Michael N. Cantor, Teal R. Furnholm, Faten Ghodhbane-Gtari, Lynne Goodwin, Alex Copeland, Maher Gtari, Marcel Huntemann, Natalia Ivanova, Nikos Kyrpides, Victor Markowitz, Kostas Mavrommatis, Natalia Mikhailova, Imen Nouioui, Rediet Oshone, Galina Ovchinnikova, Ioanna Pagani, Krishnaveni Palaniappan, Amrita Pati Aug 2015

Draft Genome Sequence Of Frankia Sp. Strain Dc12, An Atypical, Noninfective, Ineffective Isolate From Datisca Cannabina, Louis S. Tisa, Nicholas Beauchemin, Michael N. Cantor, Teal R. Furnholm, Faten Ghodhbane-Gtari, Lynne Goodwin, Alex Copeland, Maher Gtari, Marcel Huntemann, Natalia Ivanova, Nikos Kyrpides, Victor Markowitz, Kostas Mavrommatis, Natalia Mikhailova, Imen Nouioui, Rediet Oshone, Galina Ovchinnikova, Ioanna Pagani, Krishnaveni Palaniappan, Amrita Pati

New Hampshire Agricultural Experiment Station Publications

Frankia sp. strain DC12, isolated from root nodules of Datisca cannabina, is a member of the fourth lineage of Frankia, which is unable to reinfect actinorhizal plants. Here, we report its 6.88-Mbp high-quality draft genome sequence, with a G+C content of 71.92% and 5,858 candidate protein-coding genes.

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Aubuchon '16 Researches Impulse Control In Dogs, Kim Hill Aug 2015

Aubuchon '16 Researches Impulse Control In Dogs, Kim Hill

News and Events (Discontinued Series)

No abstract provided.

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Performance In The Chaordic Age: By Design Or By Destiny?, Raymond L. Forbes Aug 2015

Performance In The Chaordic Age: By Design Or By Destiny?, Raymond L. Forbes

All Faculty and Staff Scholarship

This paper discusses the challenge of performance in an age characterized by high levels of both organizational and environmental uncertainty. Today, business, not-for-profit, and governmental institutions all find themselves besieged by difficult-to-predict periods of disorder and relative certitude. A new structural form, called the chaordic organization, has arisen as one means of operating in such a turbulent and indeterminate environment. This article will consider the chaos-certainty question, examine the concepts of performance and potential, deliberate whether performance can be influenced by calculated design measures, and look at current thinking related to motivation and its relationship to organizational performance. Additionally, a …

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Student Athlete Learns Business Side Of Practice During Internship, Kim Hill Aug 2015

Student Athlete Learns Business Side Of Practice During Internship, Kim Hill

News and Events (Discontinued Series)

No abstract provided.

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A Hypothetical: Quinlan Under Ohio Law, Wendy C. Gerzog Aug 2015

A Hypothetical: Quinlan Under Ohio Law, Wendy C. Gerzog

Akron Law Review

WITH THE DECISION by all of the respondents in In re Quinlan not to appeal their case to the United States Supreme Court, the people in this country will have to wait for a definitive statement of law on a person's right to die and on a guardian's standing to assert that right for his ward. Because of the dearth of precedent in this area, each state court that is faced with the prospect of reviewing a case like Quinlan will have to grapple with its own constitutional and statutory schemes in order to make a determination of these difficult …

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A Hypothetical: Quinlan Under Ohio Law, Wendy C. Gerzog Aug 2015

A Hypothetical: Quinlan Under Ohio Law, Wendy C. Gerzog

Akron Law Review

WITH THE DECISION by all of the respondents in In re Quinlan1 not to appeal their case to the United States Supreme Court,2 the people in this country will have to wait for a definitive statement of law on a person's right to die and on a guardian's standing to assert that right for his ward. Because of the dearth of precedent in this area, each state court that is faced with the prospect of reviewing a case like Quinlan will have to grapple with its own constitutional and statutory schemes in order to make a determination of these difficult …

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Federal Income Tax Developments: 1975 Aug 2015

Federal Income Tax Developments: 1975

Akron Law Review

FEDERAL INCOME TAX DEVELOPMENTS: 1975 is the third of an annual series of articles to be published in the Winter Issue of the Akron LAW REVIEW. The thrust of this article is not only to identify the new developments, but also to trace these concepts through their formative stages. The area of concentration for this article includes cases decided through September 30, 1975. Given the volatile nature of taxation, it is crucial for the practitioner in this field to remain current with the changes which have occurred during the tax year. The purpose of this article is to highlight for …

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Open-Source Photometric System For Enzymatic Nitrate Quantification, Ben T. Wittbrodt, D. A. Squires, J. Walbeck, E. Campbell, W. H. Campbell, Joshua M. Pearce Aug 2015

Open-Source Photometric System For Enzymatic Nitrate Quantification, Ben T. Wittbrodt, D. A. Squires, J. Walbeck, E. Campbell, W. H. Campbell, Joshua M. Pearce

Department of Materials Science and Engineering Publications

Nitrate, the most oxidized form of nitrogen, is regulated to protect people and animals from harmful levels as there is a large over abundance due to anthropogenic factors. Widespread field testing for nitrate could begin to address the nitrate pollution problem, however, the Cadmium Reduction Method, the leading certified method to detect and quantify nitrate, demands the use of a toxic heavy metal. An alternative, the recently proposed Environmental Protection Agency Nitrate Reductase Nitrate-Nitrogen Analysis Method, eliminates this problem but requires an expensive proprietary spectrophotometer. The development of an inexpensive portable, handheld photometer will greatly expedite field nitrate analysis to …

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2015 August 5 -- Agenda And Attachments Aug 2015

2015 August 5 -- Agenda And Attachments

Agendas and Minutes

No abstract provided.

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Volume 52, Number 51: August 05, 2015, University Of North Dakota Aug 2015

Volume 52, Number 51: August 05, 2015, University Of North Dakota

University Letter Archive

No abstract provided.

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Novel Seed Germination In Response To California’S Historic Drought May Deplete Soil Seed Banks, Amanda M. Burns, Victoria M. Lekson, Helen I. Holmlund, Stephen D. Davis Aug 2015

Novel Seed Germination In Response To California’S Historic Drought May Deplete Soil Seed Banks, Amanda M. Burns, Victoria M. Lekson, Helen I. Holmlund, Stephen D. Davis

Biology

We observed novel seed germination, without a fire cue, in an obligate seeding species of chaparral (Ceanothus megacarpus) during historic drought in California (2014). We hypothesized that premature germination was promoted by drought-induced canopy dieback among adults that allowed high radiation loads to reach normally shaded soils, cracking seed coats, facilitating water uptake. We hypothesized that because premature seed germination was late in the season (April, when December is typical) roots would not have time to penetrate deep moisture resources before the onset of summer drought, leading to excessive mortality and eventual depletion of soil seed banks. We …

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We Are...Marshall, August 5, 2015, Office Of Marshall University Communications Aug 2015

We Are...Marshall, August 5, 2015, Office Of Marshall University Communications

We Are ... Marshall: the Newsletter for Marshall University 1999-2023

No abstract provided.

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Soluble Amyloid-Beta 42 Aggregates Stimulate Cellular Inflammation Independent Of Cd47, Sanjib Karki Aug 2015

Soluble Amyloid-Beta 42 Aggregates Stimulate Cellular Inflammation Independent Of Cd47, Sanjib Karki

Dissertations

Alzheimer’s disease (AD) is a neurodegenerative disease characterized by the accumulation and deposition of aggregated amyloid-β protein (Aβ). Most of the studies indicate that Aβ1-42) and Aβ(1-40) play an important role in the neurodegenerative pathology in AD. A critical component of AD pathology is inflammation, which results from the activation of glial cells around the Aβ deposits. The goal of my dissertation research was to determine the role of Aβ aggregation in microglia-triggered inflammation and identify cellular components that mediate the inflammatory response. CD47 is a cellular receptor found on the microglia surface and it has been proposed to have …

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Effects Of Hydrogen Peroxide On Different Toxigenic And Atoxigenic Isolates Of Aspergillus Flavus, Jake C. Fountain, Brian T. Scully, Zhi Yuan Chen, Scott E. Gold, Anthony E. Glenn, Hamed K. Abbas, R. Dewey Lee, Robert C. Kemerait, Baozhu Guo Aug 2015

Effects Of Hydrogen Peroxide On Different Toxigenic And Atoxigenic Isolates Of Aspergillus Flavus, Jake C. Fountain, Brian T. Scully, Zhi Yuan Chen, Scott E. Gold, Anthony E. Glenn, Hamed K. Abbas, R. Dewey Lee, Robert C. Kemerait, Baozhu Guo

Faculty Publications

Drought stress in the field has been shown to exacerbate aflatoxin contamination of maize and peanut. Drought and heat stress also produce reactive oxygen species (ROS) in plant tissues. Given the potential correlation between ROS and exacerbated aflatoxin production under drought and heat stress, the objectives of this study were to examine the effects of hydrogen peroxide (H2O2)-induced oxidative stress on the growth of different toxigenic (+) and atoxigenic (−) isolates of Aspergillus flavus and to test whether aflatoxin production affects the H2O2 concentrations that the isolates could survive. Ten isolates were tested: NRRL3357 (+), A9 (+), AF13 (+), Tox4 …

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Same-Sex Marriage And Jewish Law: Time For A New Paradigm?, Doron M. Kalir Aug 2015

Same-Sex Marriage And Jewish Law: Time For A New Paradigm?, Doron M. Kalir

Law Faculty Articles and Essays

In recent years the Supreme Court, as well as important segments of society, has come to accept and even celebrate same-sex relations that, in the past, and for some still today, have generated contempt, hostility, and violence. This change in law and culture poses a unique challenge for those who are moved by the plight of gay people yet concomitantly feel bound by their religious convictions and therefore prevented from providing religious legitimacy to people who yearn to be part of their community. Professor Kalir meets this challenge by proposing that the Torah (and Jewish law), read in context, accepts …

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Comparative Sorption Of Methylene Blue Onto Hydrophobic Clays, Alvaro D. Sponza, Natalia J. Fernandez, David Yang, Karla A. Ortiz, Abel E. Navarro Aug 2015

Comparative Sorption Of Methylene Blue Onto Hydrophobic Clays, Alvaro D. Sponza, Natalia J. Fernandez, David Yang, Karla A. Ortiz, Abel E. Navarro

Publications and Research

Chemical modifications of clay to remove methylene blue (MB) from aqueous solutions at room temperature were compared. Natural bentonite (NC) was modified by cation exchange with hexadecyltrimethylammonium chloride (HC), bencyltriethylammonium chloride (BC), and tetramethylammonium chloride (TC) to reverse the surface polarity of the hydrophilic bentonite. The adsorption of MB was studied and fitted by the adsorption theories of Langmuir, Freundlich, Dubinin-Radushkevich, and Temkin. Equilibrium parameters were calculated, indicating that chemical modification did not improve the adsorption, due to the electrostatic adsorption mechanism. Specific surface area was determined, reporting the following trend: NC > TC > BC > HC. Isotherms show that TC is …

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How Diverse Detrital Environments Influence Nutrient Stoichiometry Between Males And Females Of The Co-Occurring Container Mosquitoes Aedes Albopictus, Ae. Aegypti, And Culex Quinquefasciatus, Donald A. Yee, Michael G. Kaufman, Nnaemeka F. Ezeakacha Aug 2015

How Diverse Detrital Environments Influence Nutrient Stoichiometry Between Males And Females Of The Co-Occurring Container Mosquitoes Aedes Albopictus, Ae. Aegypti, And Culex Quinquefasciatus, Donald A. Yee, Michael G. Kaufman, Nnaemeka F. Ezeakacha

Faculty Publications

Allocation patterns of carbon and nitrogen in animals are influenced by food quality and quantity, as well as by inherent metabolic and physiological constraints within organisms. Whole body stoichiometry also may vary between the sexes who differ in development rates and reproductive allocation patterns. In aquatic containers, such as tree holes and tires, detrital inputs, which vary in amounts of carbon and nitrogen, form the basis of the mosquito-dominated food web. Differences in development times and mass between male and female mosquitoes may be the result of different reproductive constraints, which could also influence patterns of nutrient allocation. We examined …

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