Digital Commons Network^™

Novel Pcr Assays Complement Laser Biosensor-Based Method And Facilitate Listeria Species Detection From Food, Kwang-Pyo Kim, Atul K. Singh, Xingjian Bai, Lena Leprun, Arun K. Bhunia

Department of Food Science Faculty Publications

The goal of this study was to develop the Listeria species-specific PCR assays based on a house-keeping gene (lmo1634) encoding alcohol acetaldehyde dehydrogenase (Aad), previously designated as Listeriaadhesion protein (LAP), and compare results with a label-free light scattering sensor, BARDOT (bacterial rapid detection using optical scattering technology). PCR primer sets targeting the lap genes from the species ofListeria sensu stricto were designed and tested with 47 Listeria and 8 non-Listeria strains. The resulting PCR primer sets detected either all species of Listeria sensu stricto or individual L. innocua, L. ivanovii and L. seeligeri, …

Probiotics And Methods Of Obtaining Same, Jens Walter, Bob Thomas, Thomas Burkey

Department of Food Science and Technology: Faculty Publications

This disclosure describes novel probiotics, and also describes novel methods by which such probiotics can be obtained.

Dissecting Tumor Metabolic Heterogeneity: Telomerase And Large Cell Size Metabolically Define A Sub-Population Of Stem-Like, Mitochondrial-Rich, Cancer Cells., Rebecca Lamb, Bela Ozsvari, Gloria Bonuccelli, Duncan L Smith, Richard Pestell, Ubaldo E. Martinez-Outshoorn, Md, Robert B Clarke, Federica Sotgia, Michael P Lisanti

Kimmel Cancer Center Faculty Papers

Tumor cell metabolic heterogeneity is thought to contribute to tumor recurrence, distant metastasis and chemo-resistance in cancer patients, driving poor clinical outcome. To better understand tumor metabolic heterogeneity, here we used the MCF7 breast cancer line as a model system to metabolically fractionate a cancer cell population. First, MCF7 cells were stably transfected with an hTERT-promoter construct driving GFP expression, as a surrogate marker of telomerase transcriptional activity. To enrich for immortal stem-like cancer cells, MCF7 cells expressing the highest levels of GFP (top 5%) were then isolated by FACS analysis. Notably, hTERT-GFP(+) MCF7 cells were significantly more efficient at …

In Vivo Capture And Label-Free Detection Of Early Metastatic Cells, Samira M. Azarin, Ji Yi, Robert Michael Gower, Brian A. Aguado, Megan E. Sullivan, Ashley G. Goodman, Eric J. Jiang, Shreyas S. Rao, Yinying Ren, Susan L. Tucker, Vadim Backman, Jacqueline S. Jeruss, Lonnie D Shea

Faculty Publications

Breast cancer is a leading cause of death for women, with mortality resulting from metastasis. Metastases are often detected once tumour cells affect the function of solid organs, with a high disease burden limiting effective treatment. Here we report a method for the early detection of metastasis using an implanted scaffold to recruit and capture metastatic cells in vivo, which achieves high cell densities and reduces the tumour burden within solid organs 10-fold. Recruitment is associated with infiltration of immune cells, which include Gr1hiCD11b+cells. We identify metastatic cells in the scaffold through a label-free detection system using inverse spectroscopic …

Alumni To Be Honored During Homecoming, Emily Phelps

News and Events (Discontinued Series)

No abstract provided.

Board Of Directors Meeting Minutes (September 8, 2015), Dmacc

Board of Directors Meeting Minutes

This file contains the minutes from the Des Moines Area Community College Board meeting held on September 8, 2015.

The Bg News September 08, 2015, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. September 08, 2015. Volume 95-Issue 6.

Characterization Of Leucetta Prolifera, A Calcarean Cyanosponge From South-Western Australia, And Its Symbionts, J Fromont, Mj Huggett, Sk Lengger, K Grice, Chl Schönberg

School of Geography, Earth and Environmental Sciences

The biology and ecology of calcarean sponges are not as well understood as they are for demosponges. Here, in order to gain new insights, particularly about symbiotic relationships, the calcarean sponge Leucetta prolifera was sampled from south-western Australia and examined for its assumed photosymbionts. Pulse amplitude modulated fluorometry and extraction of photopigments established that the sponge was photosynthetic. Molecular analysis of the bacterial symbionts via sequencing of the V1–V3 region of the 16S rDNA gene confirmed that between 5 and 22% of all sequences belonged to the phylum Cyanobacteria, depending on the individual sample, with the most dominant strain aligning …

Cottonwood Environmental Law Center V. United States Forest Service, Maresa A. Jenson

Public Land & Resources Law Review

Overturning thirty-year-old precedent, Cottonwood Environmental Law Center v. United States Forest Service held that merely proving a procedural violation of the ESA is no longer enough to show irreparable injury in support of injunctive relief. The Ninth Circuit affirmed summary judgment for Cottonwood, concluding the Forest Service violated the ESA by not reinitiating consultation, but declined to provide injunctive relief because Cottonwood failed to show irreparable injury to the Canadian lynx.

Special Programs, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

Oswald Research And Creativity Program, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

Undergraduate Awards And Honors, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

Uk Undergraduate Research Program, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

Summer Research And Creativity Grants, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

Beckman Scholar 2006-07, Megan Culler

Kaleidoscope

No abstract provided.

Beckman Scholars Program, Uk Office Of Undergraduate Research

Kaleidoscope

No abstract provided.

Prehistoric Drawings In Mammoth Cave, Logan Kistler

Kaleidoscope

During a recent Earthwatch Institute survey of archaeological remains in Mammoth Cave, a project was begun to find and record prehistoric images on the cave walls. I chose to analyze petroglyphs and pictographs on three panels in Main Cave. This article offers a hypothesis for the circumstances surrounding the rock art’s production: the geometric and anthropomorphic figures in Mammoth Cave are representative of a series of visual percepts experienced cross-culturally and caused by various conditions — including sensory deprivation, fatigue, and psychoactive drug use — acting on the ocular anatomy and nervous system. That is, the glyphs might be visual …

Nietzsche Contra Wright: On Becoming What You Are, Jordan Rodgers

Kaleidoscope

Robert Wright’s recent book on evolutionary psychology, The Moral Animal, is concerned largely with the ethical implications of recent evolutionary science, and espouses a form of utilitarianism as the ethical theory that should naturally follow evolutionary insights into human psychology. This paper challenges that notion, with constant reference to the work of the philosopher Friedrich Nietzsche, on the basis that such an ethical theory places far too little emphasis on the individual as such, and is tantamount to a form of nihilism. This paper also argues that, while seeking for the happiness of other people is a good thing, our …

A Political Quagmire, Vinay Srinivasan

Kaleidoscope

This paper is intended to characterize the precarious situation the House of Saud is facing. By exploring previous historical and current events, this analysis provides an insight into the complex web the House of Saud has woven; analysis of these events allows a glimpse into the future for this political regime and the uncertainty it faces. This paper is not intended to offer a solution or support a particular course of action, group, or individual.

Complete Issue Of Volume 6

Kaleidoscope

No abstract provided.

Welcome From The President, Lee T. Todd Jr.

Kaleidoscope

No abstract provided.

Gift For Lebensborn Children

Bulmash Family Holocaust Collection

Front: Brown envelope with red cross and SS thunderbolts in the middle, with text above and below the cross. A Christmas gift envelope from the Bad Polzin-Lebensborn Home in Pomerania.

Information Provided by Michael D. Bulmash:

A Christmas (referred to by SS as Julfest) gift envelope from the Bad Polzin Lebensborn Home in Pomerania, situated in a medieval castle. The bag would be filled with a little gift and presented to children. The mothers would be living at the facility during the holiday. This particular home was opened in 1938. Under Himmler's direction after 1935, and as part of the …

Effect Of Quercetin, Genistein And Kaempferol On Glutathione And Glutathione-Redox Cycle Enzymes In 3t3-L1 Preadipocytes, William Y. Boadi, Paul K. Amartey, Andrew Lo

Chemistry Faculty Research

Context and objective: Many studies have shown that cellular redox potential is largely determined by glutathione (GSH), which accounts for more than 90% of cellular nonprotein thiols. The aim of this study was to delineate the effect of three flavonoids – namely, quercetin, kaempferol and genistein – and exogenous GSH on oxidative damage by the Fenton’s pathway through the GSH and GSH-redox cycle enzymes in 3T3-L1 cells. Materials and methods: 3T3-L1 preadipocytes were exposed to each flavonoid and GSH at concentrations of 0, 5, 10, 15, 20 and 25 µM and then GSH levels and activities of glutathione peroxidase (GSH-Px), …

September 7, 2015, James Madison University

The Breeze, 2010-2019

No abstract provided.

The Tiger Vol. 109 Issue 31 2015-09-07, Clemson University

Tiger Newspapers 2015

No abstract provided.

Efficient Photochemical Synthesis Of Peptide-Α-Phenylthioesters, Andrew Pardo, Tyrone J. Hogenauer, Zhefeng Cai, Julian A. Vellucci, Efrain M. Castillo, Carl W. Dirk, Andreas H. Franz, Katja Michael

College of the Pacific Faculty Articles

Low yields and substantial epimerization of peptide-α-thioesters often compromise the overall efficiency of native chemical ligation (NCL). Peptide arylthioesters are more reactive than peptide alkylthioesters in NCL, but are also more difficult to handle due to their propensity to hydrolyze, and are therefore often generated in situ. However, pre-prepared peptide arylthioesters are required for some NCL applications. Here we present a 7-nitroindoline-based photochemical method that generates protected peptide phenylthioesters under neutral reaction conditions via their activated esters from photoreactive peptide precursors in high isolated yields, and with low levels of epimerization. This method is fully compatible with Fmoc-strategy solid-phase peptide …

2015-September-07-Amanda Johnson, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Analysis Of A Clinically Failed, Mechanically Intact, Reconstructive Compression Plate, Bharadwaj Cheruvu, Sunil Karmacharya, Richard T. Laughlin, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

A reconstructive orthopedic bone plate was submitted for analysis. Traditional failure analysis methods were used to assess the mode of the plate failure. Metallographic investigation of the plate was carried out in this report. Since limited data was available in the literature and clinical data related to subject demography, date of removal as well as reasons for removal unknown, in depth analysis was not possible. However, the plate was received in in-tact condition with minor biological deposits and scratches, it is speculated that the failure of the plate may have been due to biological/clinical reasons, likely infection, rejecting the device …

The Ftc Has A Dog In The Patent Monopoly Fight: Will Antitrust’S Bite Kill Generic Challenges?, Jennifer D. Cieluch

Duke Law & Technology Review

Antitrust laws have been notoriously lenient in the patent realm, the underlying reason being that patents’ grant of exclusion create monopolies that defy antitrust laws in order to incentivize innovation. Thus, antitrust violations have rarely been found in the patent cases. But after the Supreme Court’s holding in FTC v. Actavis, brand name pharmaceutical companies may need to be more cautious when settling Hatch-Waxman litigation with potential patent infringers. Both brand-name drug manufacturers and generic drug manufacturers have incentives to settle cases by having the brand-name pay the generic in exchange for delaying their entry into the market. While courts …

Precise Repair Of Mping Excision Sites Is Facilitated By Target Site Duplication Derived Microhomology, David M. Gilbert, M. Catherine Bridges, Ashley E. Strother, Courtney E. Burckhalter, James M. Burnette Iii, C. Nathan Hancock

Faculty Publications

A key difference between the Tourist and Stowaway families of miniature inverted repeat transposable elements (MITEs) is the manner in which their excision alters the genome. Upon excision, Stowaway-like MITEs and the associated Mariner elements usually leave behind a small duplication and short sequences from the end of the element. These small insertions or deletions known as “footprints” can potentially disrupt coding or regulatory sequences. In contrast, Tourist-like MITEs and the associated PIF/Pong/Harbinger elements generally excise precisely, returning the genome to its original state. The purpose of this study was to determine the mechanisms underlying these …