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Differential Effects Of Munc18s On Multiple Degranulation-Relevant Trans-Snare Complexes, Hao Xu, Matthew Grant Arnold, Sushmitha Vijay Kumar

Faculty Publications

Mast cell exocytosis, which includes compound degranulation and vesicle-associated piecemeal degranulation, requires multiple Q- and R- SNAREs. It is not clear how these SNAREs pair to form functional trans-SNARE complexes and how these trans-SNARE complexes are selectively regulated for fusion. Here we undertake a comprehensive examination of the capacity of two Q-SNARE subcomplexes (syntaxin3/SNAP-23 and syntaxin4/SNAP-23) to form fusogenic trans-SNARE complexes with each of the four granule-borne R-SNAREs (VAMP2, 3, 7, 8). We report the identification of at least six distinct trans-SNARE complexes under enhanced tethering conditions: i) VAMP2/syntaxin3/SNAP-23, ii) VAMP2/syntaxin4/SNAP-23, iii) VAMP3/syntaxin3/SNAP-23, iv) VAMP3/syntaxin4/SNAP-23, v) VAMP8/syntaxin3/SNAP-23, and vi) VAMP8/syntaxin4/SNAP-23. …

Mechanistic Insights Into Glucan Phosphatase Activity Against Polyglucan Substrates, David A. Meekins, Madushi Raththagala, Kyle D. Auger, Benjamin D. Turner, Diana Santelia, Oliver Kötting, Matthew S. Gentry, Craig W. Vander Kooi

Molecular and Cellular Biochemistry Faculty Publications

Glucan phosphatases are central to the regulation of starch and glycogen metabolism. Plants contain two known glucan phosphatases, Starch EXcess4 (SEX4) and Like Sex Four2 (LSF2), which dephosphorylate starch. Starch is water-insoluble and reversible phosphorylation solubilizes its outer surface allowing processive degradation. Vertebrates contain a single known glucan phosphatase, laforin, that dephosphorylates glycogen. In the absence of laforin, water-soluble glycogen becomes insoluble, leading to the neurodegenerative disorder Lafora Disease. Because of their essential role in starch and glycogen metabolism glucan phosphatases are of significant interest, yet a comparative analysis of their activities against diverse glucan substrates has not been established. …

September 18, 2015, The Dakota Student

The Dakota Student

No abstract provided.

September 18, 2015, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.

Structural And Functional Characterization Of A Lytic Polysaccharide Monooxygenase With Broad Substrate Specificity, Anna S. Borisova, Trine Isaksen, Maria Dimarogona, Abhishek A. Kognole, Geir Mathiesen, Anikó Várnai, Åsmund K. Røhr, Christina M. Payne, Morten Sørlie, Mats Sandgren, Vincent G. H. Eijsink

Chemical and Materials Engineering Faculty Publications

The recently discovered lytic polysaccharide monooxygenases (LPMOs) carry out oxidative cleavage of polysaccharides and are of major importance for efficient processing of biomass. NcLPMO9C from Neurospora crassa acts both on cellulose and on non-cellulose β-glucans, including cellodextrins and xyloglucan. The crystal structure of the catalytic domain of NcLPMO9C revealed an extended, highly polar substrate-binding surface well suited to interact with a variety of sugar substrates. The ability of NcLPMO9C to act on soluble substrates was exploited to study enzyme-substrate interactions. EPR studies demonstrated that the Cu²⁺ center environment is altered upon substrate binding, whereas isothermal titration …

Females Sample More Males At High Nesting Densities, But Ultimately Obtain Less Attractive Mates, R. M. Tinghitella, Chelsea Stehle, Janette W. Boughman

Biological Sciences: Faculty Scholarship

Background: Sexual selection is largely driven by the availability of mates. Theory predicts that male competition and female choice should be density-dependent, with males competing more intensely at relatively high density, and females becoming increasingly discriminating when there are more males from whom to choose. Evidence for flexible mating decisions is growing, but we do not understand how environmental variation is incorporated into mate sampling strategies. We mimicked threespine stickleback (Gasterosteus aculeatus) breeding conditions in pools with high and low densities of nesting males and allowed females to search for mates to determine whether 1) mate search strategies change with …

Molecular Helices As Electron Acceptors In High-Performance Bulk Heterojunction Solar Cells, Yu Zhong, M. Tuan Trinh, Rongsheng Chen, Geoffrey E. Purdum, Petr P. Khlyabich, Melda Sezen, Seokjoon Oh, Haiming Zhu, Brandon Fowler, Boyuan Zhang, Wei Wang, Chang-Yong Nam, Matthew Y. Sfeir, Charles T. Black, Michael L. Steigerwald, Yueh-Lin Loo, Fay Ng, X.-Y. Zhu, Colin Nuckolls

Publications and Research

Despite numerous organic semiconducting materials synthesized for organic photovoltaics in the past decade, fullerenes are widely used as electron acceptors in highly efficient bulk-heterojunction solar cells. None of the non-fullerene bulk heterojunction solar cells have achieved efficiencies as high as fullerene-based solar cells. Design principles for fullerene-free acceptors remain unclear in the field. Here we report examples of helical molecular semiconductors as electron acceptors that are on par with fullerene derivatives in efficient solar cells. We achieved an 8.3% power conversion efficiency in a solar cell, which is a record high for non-fullerene bulk heterojunctions. Femtosecond transient absorption spectroscopy revealed …

Height And Body Mass On The Mating Market: Associations With Number Of Sex Partners And Extra-Pair Sex Among Heterosexual Men And Women Aged 18–65, David A. Frederick, Brooke N. Jenkins

Psychology Faculty Articles and Research

People with traits that are attractive on the mating market are better able to pursue their preferred mating strategy. Men who are relatively tall may be preferred by women because taller height is a cue to dominance, social status, access to resources, and heritable fitness, leading them to have more mating opportunities and sex partners. We examined height, education, age, ethnicity, and body mass index (BMI) as predictors of sexual history among heterosexual men and women (N = 60,058). The linear and curvilinear associations between self-reported height and sex partner number were small for men when controlling for education, BMI, …

Lysyl Hydroxylase 3 Localizes To Epidermal Basement Membrane And Is Reduced In Patients With Recessive Dystrophic Epidermolysis Bullosa., Stephen A Watt, Jasbani H S Dayal, Sheila Wright, Megan Riddle, Celine Pourreyron, James R Mcmillan, Roy M Kimble, Marco Prisco, Ulrike Gartner, Emma Warbrick, W H Irwin Mclean, Irene M Leigh, John A Mcgrath, Julio C Salas-Alanis, Jakub Tolar, Andrew P South

Department of Dermatology and Cutaneous Biology Faculty Papers

Recessive dystrophic epidermolysis bullosa (RDEB) is caused by mutations in COL7A1 resulting in reduced or absent type VII collagen, aberrant anchoring fibril formation and subsequent dermal-epidermal fragility. Here, we identify a significant decrease in PLOD3 expression and its encoded protein, the collagen modifying enzyme lysyl hydroxylase 3 (LH3), in RDEB. We show abundant LH3 localising to the basement membrane in normal skin which is severely depleted in RDEB patient skin. We demonstrate expression is in-part regulated by endogenous type VII collagen and that, in agreement with previous studies, even small reductions in LH3 expression lead to significantly less secreted LH3 …

Preparation And Characterization Of Anti-Hiv Nanodrug Targeted To Microfold Cell Of Gut-Associated Lymphoid Tissue, Upal Roy, Hong Ding, Sudheesh Pilakka-Kanthikeel, Andrea D. Raymond, Venkata Atluri, Adriana Yndart, Elena M. Kaftanovskaya, Elena Batrakova, Marisela Agudelo, Madhavan Nair

Health & Biomedical Sciences Faculty Publications

The human immunodeficiency virus 1 (HIV-1) still remains one of the leading life-threatening diseases in the world. The introduction of highly active antiretroviral therapy has significantly reduced disease morbidity and mortality. However, most of the drugs have variable penetrance into viral reservoir sites, including gut-associated lymphoid tissue (GALT). Being the largest lymphoid organ, GALT plays a key role in early HIV infection and host–pathogen interaction. Many different treatment options have been proposed to eradicate the virus from GALT. However, it becomes difficult to deliver traditional drugs to the GALT because of its complex physiology. In this regard, we developed a …

Marriage The Divine And Blessed Walk Of Life, Robert Weise

Concordia Journal

Marriage is God’s divine and blessed walk of life for man and woman being true to each other, to be fruitful, to beget children, and to nurture and bring them up to the glory of God.

Engaging Our Culture Faithfully, Harold Senkbeil

Concordia Journal

If the problem we face is not the secularization of society but the secularization of the church brought about by importing expressive individualism into the church, then we simply cannot face contemporary challenges individually.

Lanthorn, Vol. 50, No. 07, September 17, 2015, Grand Valley State University

Volume 50, July 13, 2015 - June 6, 2016

Lanthorn is Grand Valley State's student newspaper, published from 1968 to the present.

The Firece Green Fire: Vol. 6 Issue 2, Wofford College Environmental Studies Program

The Fierce Green Fire

No abstract provided.

Weak Tradeoff Between Xylem Safety And Xylem-Specific Hydraulic Efficiency Across The World's Woody Plant Species., Sean M Gleason, Mark Westoby, Steven Jansen, Brendan Choat, Uwe G Hacke, Robert B Pratt, Radika Bhaskar, Tim J Brodribb, Sandra J Bucci, Kun-Fang Cao, Hervé Cochard, Sylvain Delzon, Jean-Christophe Domec, Ze-Xin Fan, Taylor S Feild, Anna L Jacobsen, Daniel M Johnson, Frederic Lens, Hafiz Maherali, Jordi Martínez-Vilalta, Stefan Mayr, Katherine A Mcculloh, Maurizio Mencuccini, Patrick J Mitchell, Hugh Morris, Andrea Nardini, Jarmila Pittermann, Lenka Plavcová, Stefan G Schreiber, John S Sperry, Ian J Wright, Amy E Zanne

Anatomy and Regenerative Biology Faculty Publications

The evolution of lignified xylem allowed for the efficient transport of water under tension, but also exposed the vascular network to the risk of gas emboli and the spread of gas between xylem conduits, thus impeding sap transport to the leaves. A well-known hypothesis proposes that the safety of xylem (its ability to resist embolism formation and spread) should trade off against xylem efficiency (its capacity to transport water). We tested this safety-efficiency hypothesis in branch xylem across 335 angiosperm and 89 gymnosperm species. Safety was considered at three levels: the xylem water potentials where 12%, 50% and 88% of …

The Setonian, September 17, 2015, Seton Hall University

2015

No abstract provided.

Ua12/2/1 College Heights Herald, Vol. 91, No. 8, Wku Student Affairs

WKU Administration Documents

WKU campus newspaper reporting campus, athletic and Bowling Green, Kentucky news.

• Henderson, Andrew. Students Voice Opinions on Weapons
• Harney, Lashana. Gary Ransdell Clarifies Questions to Student Government Association Senate – Confucius Institute
• Pettway, Shantel. New Interfaith Campus Coalition Holds First Meeting
• Mayo, Marcel. Student Government Association Allocates Funds to Study Away Scholarships
• Matthews, Carly. Best Expectations Program Revamped to Be More Effective
• Mayo, Marcel. History Professor Makes History – John Hardin
• King, Jennifer. Editorial Cartoon Big Red & Social Media
• What’s the Catch? My Tinder Horror Story – Social Media
• Winstead, John. I Demand More Than Marriage Equality – Gays, …

The Depsipeptide Romidepsin Reverses Hiv-1 Latency In Vivo, Ole S. Søgaard, Mette E. Graversen, Steffen Leth, Rikke Olesen, Christel R. Brinkmann, Sara K. Nissen, Anne Sofie Kjaer, Mariane H. Schleimann, Paul W. Denton, William J. Hey-Cunningham, Giuseppe Pantaleo, Kim Krogsgaard, Maja Sommerfelt, Remi Fromentin, Nicolas Chomont, Thomas A. Rasmussen, Lars Østergaard, Martin Tolstrup

Biology Faculty Publications

Pharmacologically-induced activation of replication competent proviruses from latency in the presence of antiretroviral treatment (ART) has been proposed as a step towards curing HIV-1 infection. However, until now, approaches to reverse HIV-1 latency in humans have yielded mixed results. Here, we report a proof-of-concept phase Ib/IIa trial where 6 aviremic HIV-1 infected adults received intravenous 5 mg/m2 romidepsin (Celgene) once weekly for 3 weeks while maintaining ART. Lymphocyte histone H3 acetylation, a cellular measure of the pharmacodynamic response to romidepsin, increased rapidly (maximum fold range: 3.7–7.7 relative to baseline) within the first hours following each romidepsin administration. Concurrently, HIV-1 transcription …

The Tiger Vol. 109 Issue 34 2015-09-17, Clemson University

Tiger Newspapers 2015

No abstract provided.

Viva La Ova - The Journey!/Jamiyah Hassan, Jamiyah Hassan

Archival Material (2010s)

The journey of a woman from the womb to the tomb starts with the little ova. The challenges of pregnancy and its potential complications can convert a naturally physiological event to something more deadly. A woman who dies during pregnancy and delivery is a tragedy that we want to avoid. For over five decades Malaysia has improved the obstetric services and maternal mortality has reduced significantly. Family planning and effective contraception will be the much needed strategy to achieve maternal mortality comparable to the more developed world.

Statins Increase Plasminogen Activator Inhibitor Type 1 Gene Transcription Through A Pregnane X Receptor Regulated Element., Frederick M Stanley, Kathryn M Linder, Timothy J Cardozo

Department of Medical Genetics Faculty Papers

Plasminogen activator inhibitor type 1 (PAI-1) is a multifunctional protein that has important roles in inflammation and wound healing. Its aberrant regulation may contribute to many disease processes such as heart disease. The PAI-1 promoter is responsive to multiple inputs including cytokines, growth factors, steroids and oxidative stress. The statin drugs, atorvastatin, mevastatin and rosuvastatin, increased basal and stimulated expression of the PAI-1 promoter 3-fold. A statin-responsive, nuclear hormone response element was previously identified in the PAI-1 promoter, but it was incompletely characterized. We characterized this direct repeat (DR) of AGGTCA with a 3-nucleotide spacer at -269/-255 using deletion and …

Vista: September 17, 2015, University Of San Diego

USD Vista (1965-)

No abstract provided.

Alternative A - Biology, University Of Alabama In Huntsville

Graduate Program Checklists

No abstract provided.

Conceptualization Of Arctic Tundra Landscape Transitions Using The Alaska Thermokarst Model, W. Robert Bolton, Vladimir Romanovsky, A. David Mcguire, Mark Lara

Research Posters

Thermokarst topography forms whenever ice-rich permafrost thaws and the ground subsides due to the volume loss when excess ice transitions to water. The Alaska Thermokarst Model (ATM) is a large-scale, state-and-transition model designed to simulate landscape transitions between landscape units, or cohorts, due to thermokarst. The ATM uses a frame-based methodology to track transitions and proportion of cohorts within a 1-km2 grid cell. In the arctic tundra environment, the ATM tracks landscape transitions between non-polygonal ground (meadows), low center polygons, coalescent low center polygons, flat center polygons, high center polygons, ponds and lakes. The transition from one terrestrial landscape type …

Synthetic Anthocyanidins And Their Antioxidant Properties, Homar S. Barcena, Peishan Chen, Abraham Tuachi

Publications and Research

Anthocyanidins were synthesized to study the effect of methoxy substitution on the B ring to their antioxidant property. Comparative FRAP studies show 2′- and 4′-methoxy substituents have higher antioxidant activities, which may be attributed to both resonance and inductive effects.Graphical abstract:Anthocyanidins as reducing agents Electronic supplementary material The online version of this article (doi:10.1186/s40064-015-1250-x) contains supplementary material, which is available to authorized users.

Analysis Of Hypersonic Vehicle Wakes, Matthew A. Kania

Theses and Dissertations

As advancements are made with ballistic missiles, particularly in the area of hypersonic bodies, there is a growing need to advance the methods of detecting these new ballistic weapons. As a result, the National Air and Space Intelligence Center has asked the Air Force Institute of Technology to examine the wake region behind hypersonic bodies. A thorough understanding of the aerothermal phenomena and the chemical reactions occurring in the wake region will enable an advancement of tracking hypersonic bodies. This research examined the wake region behind a hypersonic body using computational fluid dynamics.
This study used Pointwise® to develop a …

Wade, Chrystal, Bronx African American History Project

Oral Histories

Chrystal Wade moved to the Bronx from Harlem when she was five years old after moving from the Harlem River Drive projects to Detroit and back to New York. Chrystal’s mother gave birth to her at age 18, but she was adopted by her grandparents at age five, as they wanted to make sure she would have a good structured family life. Upon moving into a five-story walkup at 532 East 157^th Street and St. Ann’s avenue, her father and brother-in-law almost immediately had an altercation with the neighbors because her family was the second black family in the …

Modeling Radiation Effectiveness For Inactivation Of Bacillus Spores, Emily A. Knight

Theses and Dissertations

This research models and analyzes the inactivation of Bacillus spores following a radiation exposure and the process enacted by the Bacillus spore to repair the resulting damage. Irradiation of a spore and the medium surrounding the spore induces chemical reactions that produce reactive oxygen species (ROS). This research will consider the reaction- diffusion of these ROS throughout the spore. These ROS can react with the spore's DNA and enzymes to degrade them to such an extent that the DNA cannot be repaired or replicated, thus causing spore death. In order to survive a dose of radiation, a spore must repair …

Testing The Adequacy Of A Semi-Markov Process, Richard S. Seymour

Theses and Dissertations

Due to the versatility of its structure, the semi-Markov process is a powerful modeling tool used to describe complex systems. Though similar in structure to continuous time Markov chains, semi-Markov processes allow for any transition time distribution which enables these processes to t a wider range of problems than the continuous time Markov chain. While semi-Markov processes have been applied in fields as varied as biostatistics and finance, there does not exist a theoretically-based, systematic method to determine if a semi-Markov process accurately fits the underlying data used to create the model. In fields such as regression and analysis of …

Network Analysis With Stochastic Grammars, Alan C. Lin

Theses and Dissertations

Digital forensics requires significant manual effort to identify items of evidentiary interest from the ever-increasing volume of data in modern computing systems. One of the tasks digital forensic examiners conduct is mentally extracting and constructing insights from unstructured sequences of events. This research assists examiners with the association and individualization analysis processes that make up this task with the development of a Stochastic Context -Free Grammars (SCFG) knowledge representation for digital forensics analysis of computer network traffic. SCFG is leveraged to provide context to the low-level data collected as evidence and to build behavior profiles. Upon discovering patterns, the analyst …