Digital Commons Network^™

Global Analysis Of Mouse Polyomavirus Infection Reveals Dynamic Regulation Of Viral And Host Gene Expression And Promiscuous Viral Rna Editing, Seth B. Garren

Doctoral Dissertations

Murine polyomavirus lytically infects mouse cells, transforms rat cells in culture, and is highly oncogenic in rodents. We have used deep sequencing to follow polyomavirus infection of mouse NIH3T6 cells at various times after infection and analyzed both the viral and cellular transcriptomes. Alignment of sequencing reads to the viral genome illustrated the transcriptional profile of the early-to-late switch with both early-strand and late-strand RNAs being transcribed at all time points. A number of novel insights into viral gene expression emerged from these studies, including the demonstration of widespread RNA editing of viral transcripts at late times in infection. By …

Shadowed By Scale: Subtle Behavioral Niche Partitioning In Two Sympatric, Tropical Breeding Albatross Species, Melinda Conners, Elliott Hazen, Daniel Costa, Scott A. Shaffer

Faculty Publications, Biological Sciences

Background To meet the minimum energetic requirements needed to support parents and their provisioned offspring, the timing of breeding in birds typically coincides with periods of high food abundance. Seasonality and synchrony of the reproductive cycle is especially important for marine species that breed in high latitudes with seasonal booms in ocean productivity. Laysan and black-footed albatrosses breeding in the northwestern Hawaiian Islands have a dual reliance on both seasonally productive waters of high latitudes and on nutrient-poor waters of low latitudes, because their foraging ranges contract during the short but critical brood-guard stage. Therefore, these species face an additional …

Social Learning: Parents May Not Always Know Best, Simon C. Griffith, Culum Brown

Social Cognition Collection

The efficiency with which animals learn new skills depends on their ability to choose good tutors. A new study shows that early-life stress causes young zebra finches to switch tutor preference from parents to unrelated adults.

A Scientific Basis For Regulating Deep-Sea Fishing By Depth, Jo Clarke, Rosanna Milligan, David M. Bailey, Francis Neat

Marine & Environmental Sciences Faculty Articles

The deep sea is the world’s largest ecosystem, with high levels of biodiversity and many species that exhibit life-history characteristics thatmake them vulnerable to high levels of exploitation. Many fisheries in the deep sea have a track record of being unsustainable. In the northeast Atlantic, there has been a decline in the abundance of commercial fish species since deep-sea fishing commenced in the 1970s. Current management is by effort restrictions and total allowable catch (TAC), but there remain problems with compliance and high levels of bycatch of vulnerable species such as sharks. The European Union is currently considering new legislation …

College Voice Vol. 99 No. 1, Connecticut College

2015-2016

No abstract provided.

Daily Eastern News: September 21, 2015, Eastern Illinois University

September

No abstract provided.

Uab Is Nih Center Of Excellence For Biology Of Aging Research, Traci Bratton

Browse All News

No abstract provided.

Clinically Available Medicines Demonstrating Anti-Toxoplasma Activity, Andrew J. Neville, Sydney J. Zach, Xiaofang Wang, Joshua L. Larson, Abigail K. Judge, Lisa A. Davis, Jonathan L. Vennerstrom, Paul H. Davis

Biology Faculty Publications

Toxoplasma gondii is an apicomplexan parasite of humans and other mammals, including livestock and companion animals. While chemotherapeutic regimens, including pyrimethamine and sulfadiazine regimens, ameliorate acute or recrudescent disease such as toxoplasmic encephalitis or ocular toxoplasmosis, these drugs are often toxic to the host. Moreover, no approved options are available to treat infected women who are pregnant. Lastly, no drug regimen has shown the ability to eradicate the chronic stage of infection, which is characterized by chemoresistant intracellular cysts that persist for the life of the host. In an effort to promote additional chemotherapeutic options, we now evaluate clinically available …

Crow's Nest, September 21, 2015, Crow's Nest Staff

Crow's Nest

The September 21, 2015, issue of the Crow's Nest.

Hybrid Dynamic Optimization Methods For Systems Biology With Efficient Sensitivities, Nicholas Lewis, John Hedengren, Eric Haseltine

Faculty Publications

In recent years, model optimization in the field of computational biology has become a prominent area for development of pharmaceutical drugs. The increased amount of experimental data leads to the increase in complexity of proposed models. With increased complexity comes a necessity for computational algorithms that are able to handle the large datasets that are used to fit model parameters. In this study the ability of simultaneous, hybrid simultaneous, and sequential algorithms are tested on two models representative of computational systems biology. The first case models the cells affected by a virus in a population and serves as a benchmark …

2015-September-21-Sarah Kapoor, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Economic And Environmental Modeling Of An Agricultural Watershed In The Mississippi Delta: Crop Selection Using An Lp Model And Water Quality Impacts Of Bioenergy Crops Using The Annagnps Model, Rodney Eugene Wright

Student Theses and Dissertations

The conventional ethanol mandates set forth by the renewable fuel standards in the Energy Independence and Security Act (EISA) of 2007 have resulted in an increased demand for corn. The requirement has translated into a 20% increase in corn hectares in the U.S, based on the three-year period prior to 2005 to the most recent three-year period, 2012-2014. This economic study optimized crop selection on a case study farm in the Arkansas Delta with the Purdue Crop/Linear Programming model. The cropping optimization was performed at six corn price levels consisting of near the loan rate, pre-EISA, post-EISA low, post-EISA average, …

Cold Plasma Inactivation Of Bacterial Biofilms And Reduction Of Quorum Sensing Regulated Virulence Factors, Dana Ziuzina, Daniela Boehm, Sonal Patil, Patrick J. Cullen, Paula Bourke

Articles

The main objectives of this work were to investigate the effect of atmospheric cold plasma (ACP) against a range of microbial biofilms commonly implicated in foodborne and health- care associated human infections and against P. aeruginosa quorum sensing (QS)-regu- lated virulence factors, such as pyocyanin, elastase (Las B) and biofilm formation capacity post-ACP treatment. The effect of processing factors, namely treatment time and mode of plasma exposure on antimicrobial activity of ACP were also examined. Antibiofilm activity was assessed for E. coli, L. monocytogenes and S. aureus in terms of reduction of cultur- ability and retention of metabolic activity using …

The Free Press Vol. 47, Issue No. 03, 09-21-2015, Krysteana Scribner

Free Press, The

UMaine system moving towards more efficient energy -- Smart Forest Initiative to begin in Hemlock Forest -- Cyber security degree launched in UMaine System -- USM High School hopes to increase college enrollment -- Pepper spray discharged in lower Brooks -- Portland women’s history gets its own app made by professor

2015-September-21-Sarah Kapoor, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

September 21, 2015, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.

Disc In Flames: Roles Of Tnf-Α And Il-1Β In Intervertebral Disc Degeneration., Z I Johnson, Z R Schoepflin, H Choi, I M Shapiro, M V Risbud

Department of Orthopaedic Surgery Faculty Papers

The intervertebral disc is an important mechanical structure that allows range of motion of the spinal column. Degeneration of the intervertebral disc--incited by aging, traumatic insult, genetic predisposition, or other factors--is often defined by functional and structural changes in the tissue, including excessive breakdown of the extracellular matrix, increased disc cell senescence and death, as well as compromised biomechanical function of the tissue. Intervertebral disc degeneration is strongly correlated with low back pain, which is a highly prevalent and costly condition, significantly contributing to loss in productivity and health care costs. Disc degeneration is a chronic, progressive condition, and current …

Faculty Senate Bill Fsb-2015-09-21-01: University Curriculum Committee, Armstrong State University

Armstrong Faculty Senate: Bills, Motions & Resolutions (Archived)

Faculty Senate Bill FSB-2015-09-21-01: University Curriculum Committee

My News

My News (2014-2020)

• Reduced Shakespeare Company brings "The History of Comedy (abridged)" to the PAC
• Concussion expert to present Fries Lecture
• Celebrate Science, Technology, Engineering and Math at Georgia Southern on Sept. 26
• Join Eagle Nation for a Day at Turner Field
• Georgia Southern professor named among "Georgia Gamechangers in STEM Education"
• Professor heralds unsung pioneer of evolutionary biology in LeConte Lecture

Experiential Metaphors: Create Anti-Negative Home Products, Hong Ying Guo

Theses

Experiential metaphor is a user-centered design approach and methodology that I developed for my thesis project. This approach can be applied to a design to influence a user's perception or design process to facilitate the designer's concept ideation. The intention of this approach is to create a pleasurable product experience and a positive emotional response through interaction with the product and interpretation of its meaning. Grounded in meaning association, which is correlated with non-linguistic cognitive metaphor and human emotional models, four different levels of experiential metaphor are formed by the synthesis of the metaphor structure and levels of emotional elicitation. …

September 21, 2015 Armstrong Faculty Senate Agenda, Armstrong State University

Armstrong Faculty Senate: Armstrong Faculty Senate Agenda (Archived)

September 21, 2015 Armstrong Faculty Senate Agenda

September 21, 2015 Armstrong Faculty Senate Minutes, Armstrong State University

Armstrong Faculty Senate: Minutes (Archived)

September 21, 2015 Armstrong Faculty Senate Minutes

Medical School Watercooler Newsletter - September 20, 2015, Lindsey Lyle

Watercooler Newsletter

This is the September 20, 2015 edition of the Frederick P. Whiddon College of Medicine's Newsletter - Watercooler.

Contents Include:

USA to Host Welcome Reception Oct. 8

USA Welcomes Dr. Philip Almalouf

Sept. 24 DSS to Feature Dr. Lucio Miele

Clyde G. 'Sid' Huggins Medical Student Research Awards Announced

Holy Cross Magazine, Vol. 49, No. 4, Fall 2015, College Of The Holy Cross

Holy Cross Magazine

Syllabus: Spectroscopy
with Associate Dean of the College and Professor of Chemistry Ronald M. Jarret
By Pamela Reponen

A Defining Moment
Holy Cross has arrived at a moment of transformation. A new comprehensive campaign, “Become More: Campaign for the Future of Holy Cross,” imagines what Holy Cross will be in the decades to come.
By Jack O’Connell ’81

Playing it Forward
In July, Holy Cross received a record-breaking commitment—$32.5 million— from alumnus John E. Luth ’74 and his wife, Joanne Chouinard-Luth, D.M.D. Their extraordinary gift represents the single largest donation in the College’s history—and it will be used to dramatically …

[14C] Glucose Cell Wall Incorporation Assay For The Estimation Of Cellulose Biosynthesis, Chad Brabham, Jozsef Stork, Seth Debolt

Horticulture Faculty Publications

Cellulose is synthesized by Cellulose Synthase A proteins at the plasma membrane using the substrate UDP glucose. Herein, we provide a detailed method for measuring the incorporation of radiolabeled glucose into the cellulose fraction of the cell wall. In this method Arabidopsis seedlings are treated for 2 h with a cellulose biosynthesis inhibitor in the presence of radiolabeled glucose, and are subsequently boiled in acetic-nitric acid to solubilize non-cellulosic material. The radiolabeled glucose detected in the insoluble fraction indicates the amount of cellulose synthesized during the experimental timeframe. The short-term nature of this method is a useful tool in determining …

Student Ensemble: Choral Showcase, September 20, 2015, Kristin Moroni Director, Mark Grizzard Director, Karyl Carlson Director, Leslie Manfredo Director

School of Music Programs

Center for the Performing Arts
September 20, 2015
Sunday Afternoon
3:00 p.m.

Synthesis Of Polyclonal Antibodies Against Aflatoxin B1, Wiyogo Prio Wicaksono, Deni Samsudin Permana, Romsyah Maryam, Yasuaki Einaga

Makara Journal of Science

Polyclonal antibodies of aflatoxin B1 were successfully produced from New Zealand White female rabbits after immunization by the hapten of aflatoxin B1-carboxymethyl hydroxylamine hemihydrochloride (AFB1-CMO) conjugated with bovine serum albumin (BSA) as the antigen. The hapten was synthesized using the carbodiimide method with CMO as a linker. Absorption peaks at 362, 264, and 218 nm were observed as a result of characterization with UV-Vis spectroscopy, while IR spectroscopy showed peaks at 3448 cm-1 and 1642 cm-1 attributable to the hydroxyl and nitrile groups, respectively. Furthermore, mass spectrometry showed fragmentation at the m/z of 386, 368.2, and 310, which confirms that …

Enzymatic Hydrolysis Of Mannan From Konjac (Amorphophallus Sp.) Using Mannanase From Streptomyces Lipmanii To Produce Manno-Oligosaccharides, Ashadi Sasongko, Yopi, Nanik Rahmani, Puspita Lisdiyanti, Endang Saepudin

Makara Journal of Science

Mannan is an abundant polysaccharide that can be found in konjac (Amorphophallus sp.). Mannan can be enzymatically hydrolyzed using mannanase to produce manno-oligosaccharides which can be used as a prebiotic. The aims of this research are to determine the production time of mannanase from Streptomyces lipmanii, perform enzyme characterization, optimize the hydrolysis time, and characterize the hydrolysis product. A qualitative assay using the indicator Congo red showed that S. lipmanii generated a clear zone, indicating that S. lipmanii produced mannanase in konjac medium and possessed mannanolytic activity. Enzyme activity was determined through reducing sugar measurement using the dinitrosalycylic acid method, …

Identification And Bioactivity Studies Of Flavonoid Compounds From Macaranga Hispida (Blume) Mull.Arg, Megawati, Endang Saepudin, Muhammad Hanafi, Akhmad Darmawan, Puspa Dewi N. Lotulung

Makara Journal of Science

Two flavonoid compounds, 5,7,3’,4’-tetrahydroxy-6-geranylflavonol (1) and kaempferol 7-O-β-glucose (2) have been isolated from the leaves of Macaranga hispida (Blume), Mull.Arg. Isolation and purification were conducted by chromatography methods and chemical structure characterization was carried out by spectroscopic methods. The 5,7,3’,4’-tetrahydrxyi-6-geranyl flavonol (1) and kaempferol 7-O-glucose (2) had moderate cytotoxic activity against murine leukemia P-388 cell lines with IC50 value of 0.22 and 101.5 μg/mL, respectively. The IC50 for antioxidant activities of (1) and (2) were 2.83 and 13.95 μg/mL, respectively. The LC50 of (1) and (2) from BSLT were 350 and >1000 μg/mL, respectively.

Transformation Of Inhibitor Of Meristem Activity (Ima) Gene Into Jatropha Curcas L., Asri Pirade Paserang, Aris Tjahjoleksono, Utut Widyastuti, Suharsono

Makara Journal of Science

Jatropha is one of the many biodiesel plants developed in tropical countries. Efforts to increase its productivity can be done using various methods of breeding. One of the breeding methods is the introduction of genes into the Jatropha plant. The aim of this study is to assess the success of genetic transformation using the Inhibitor of Meristem Activity (IMA) gene in Jatropha curcas. The research procedures included inoculation of explants with Agrobacterium tumefaciens, callus induction, screening test of selection media, regeneration, and gene expression analysis using Polymerase Chain Reaction (PCR). IMA is one of the genes that controls flowering genes …