Digital Commons Network^™

The Generation, Exploitation And Future Of Induced Pluripotent Stem Cells, Jacob Steenwyk

Scholarly Undergraduate Research Journal at Clark (SURJ)

The foundational advancements of John Gurdon and Shinya Yamanaka have improved understanding of dedifferen- tiation of cells to a pluripotent state. The seminal discovery established a novel system to study disease pathogenesis, drug screening, and toxicity, as well as sprouted the new field of regenerative medicine. In this article, the method- ology to obtain dedifferentiated cells, known as induced pluripotent stem (iPS) cells, subsequent validation, and application of which are reviewed. The experiments investigated here aim to demonstrate the capacity of iPS cells to replace the ethically-gray human embryonic cells by developing human livers and viable, healthy animals. It is …

Biochemical Effects Of Meditation: A Literature Review, William C. Daube, Charles E. Jakobsche

Scholarly Undergraduate Research Journal at Clark (SURJ)

Meditation is an activity that can help reduce stress and anxiety from daily life as well as help cultivate overall feel- ings of peacefulness, relaxation, and contentment. This review describes several studies that have been used to assess how meditation can influence the body at the molecular level. The presented results focus on small-molecule metab- olites, which are broadly defined as naturally-produced molecules that weigh less than approximately 1000 Da. The results show that meditation can significantly affect hormones and neurotransmitters such as cortisol, dehydroepi- androstrone, serotonin, melatonin, and epinephrine. Some common and modern experimental techniques that are relevant to …

The Efficacy Of Electroconvulsive Therapy In Managing Self-Injurious Behaviors Among Youth With Autism Spectrum Disorder: A Review, Themal I. Ellawala

Scholarly Undergraduate Research Journal at Clark (SURJ)

Self-Injurious Behaviors (SIB) in Autism Spectrum Disorder (ASD) form a constellation of particularly pervasive and harmful symptoms signifying high morbidity for children and adolescents. Electroconvulsive Therapy (ECT) has been proposed as a last resort for particularly severe and treatment-resistant forms of SIB, yet has received little acceptance in mainstream clinical psychology. This paper summarizes findings of four studies investigating the effic- acy of ECT in treating SIB in ASD inflicted youth. This paper also evaluates the research on potential side-effects and ethical implications of ECT use. I conclude that while extensive further research is required to understand the implications of …

Medical School Watercooler Newsletter - October 18, 2015, Lindsey Lyle

Watercooler Newsletter

This is the October 18, 2015 edition of the Frederick P. Whiddon College of Medicine's Newsletter - Watercooler.

Contents Include:

USA Welcomes Ali Shropshire, MSN, FNP-BC

Med School Cafe - Expert Advice for the Community

USA Welcomes Dr. Raymond Langley

Dr. James W. Fleshman Jr. to Present Frazer/Leigh Memorial Lecture

Harvey Ikner Leads USA Employee Campaign for United Way

USA Medical Students Cook for Families at Ronald McDonald House

USA Medical Alumni Association Lecture to Feature COM Alum Dr. John LeDoux

USA Welcomes Dr. Steven Kahn

Craving Versus Control: Negative Urgency And Neural Correlates Of Alcohol Cue Reactivity, David S. Chester, Donald R. Lynam, Richard Milich, C. Nathan Dewall

Department of Psychological Sciences Faculty Publications

Background: Alcohol abuse is a common and costly practice. Individuals high in negative urgency, the tendency to act rashly when experiencing negative emotions, are at particular risk for abusing alcohol. Alcohol abuse among individuals high in negative urgency may be due to (a) increased activity in the brain’s striatum, (b) decreased activity in brain regions associated with self-control, or (c) a combination of the two. Methods: Thirty eight non-alcohol-dependent participants completed a measure of negative urgency and then underwent functional magnetic resonance imaging (fMRI) while passively viewing pleasant and alcohol images. Results: Alcohol images (as compared to pleasant images) were …

October 17, 2015 (Pages 6187-6258), Pennsylvania Legislative Reference Bureau

Volume 45 (2015)

No abstract provided.

Finding Springs In The File Cabinet, Mason Johnson, Ashley Ignatius

Sinkhole Conference 2015

The Minnesota Pollution Control Agency (MPCA), in partnership with other agencies, is currently undertaking comprehensive sub-basin assessments statewide over a ten-year period. Southeast Minnesota has over 17,500 kilometers of perennial and intermittent streams, making the task of comprehensive sub-basin assessment challenging; the task is further complicated by karst geology. In the summer of 2014, a pilot project began between the MPCA and Minnesota Department of Natural Resources (DNR) to digitally preserve paper documents which capture qualitative and quantitative data about the hydrology, water chemistry, geomorphology, biology, land use and karst features of southeast Minnesota streams. The paper documents in file …

Legacy Data In The Minnesota Spring Inventory, Greg Brick

Sinkhole Conference 2015

Past spring inventories have covered certain parts of Minnesota reasonably well, notably, the springs of the Minneapolis-St. Paul metropolitan area and the southeastern Minnesota karst. But hitherto, there has not been a systematic effort to create a uniform statewide inventory. The first step, before hunting down new springs, was to compile existing data and the most fruitful source of hydrological legacy data for the Minnesota spring inventory was the DNR Fisheries files. Once entered into a GIS-capable database, these spring locations can help “seed the ground” so that when crews finally do take to the field to map more springs, …

A Proposed Hypogenic Origin Of Iron Ore Deposits In Southeast Minnesota Karst, E. Calvin Alexander Jr., Betty J. Wheeler

Sinkhole Conference 2015

From 1942 through 1968 there was an active iron ore mining industry in western Fillmore, eastern Mower and southern Olmsted Counties of Minnesota. This iron mining district was 250 miles south of, and the ores were a billion years younger than, the ores of the classic iron mining districts in northern Minnesota. The high grade iron ore was mostly goethite and hematite and occurred as near-surface relatively small pods which unconformably filled paleokarst depressions in the Devonian Spillville Formation and the Ordovician Stewartville Formation. The source of the iron has long been cryptic. The available field and textural evidence is …

Using Nitrate, Chloride, Sodium, And Sulfate To Calculate Groundwater Age, Kimm Crawford, Terry Lee

Sinkhole Conference 2015

Regression analysis is used to identify monotonic trends to assign water age using ion data from two large well water databases from southeast Minnesota (SE MN). Nitrate (NO3-N), chloride (Cl), sodium (Na), and sulfate (SO4) ions in the commonly used aquifers in SE MN can be used as groundwater tracers since they are either entirely or partly anthropogenic in their sources, their loading occurs on a regional scale, and they are almost entirely conserved. Ion concentrations over time are used to establish six trend patterns. Two patterns are unchanging (background and stable above background), and four are changing (linear up, …

Hydrologic And Geochemical Dynamics Of Vadose Zone Recharge In A Mantled Karst Aquifer: Results Of Monitoring Drip Waters In Mystery Cave, Minnesota, Daniel H. Doctor, E. Calvin Alexander Jr., Roy Jameson, Scott C. Alexander

Sinkhole Conference 2015

Caves provide direct access to flows through the vadose zone that recharge karst aquifers. Although many recent studies have documented the highly dynamic processes associated with vadose zone flows in karst settings, few have been conducted in mantled karst settings, such as that of southeastern Minnesota. Here we present some results of a long-term program of cave drip monitoring conducted within Mystery Cave, Minnesota. In this study, two perennial ceiling drip sites were monitored between 1997 and 2001. The sites were located about 90 m (300 ft) apart along the same cave passage approximately 18 m (60 ft) below the …

Earthonomics: Balancing Between Earth And Business, Yazen Abdin

Environmental and Earth Law Journal (EELJ)

Economists consistently fail to account that Earth has a finite amount of resources. This article analyzes the behavior of corporate America and the effect it has on the world form an Earth jurisprudence standpoint. It will explore twp specific industries, oil and food, and what role they have played in the environment's health. It also explores potential solutions.

Responding To The Great Work: The Role Of Earth Jurisprudence And Wild Law In The 21st Century, Dr. Michelle Maloney, Sister Pat Siemen Op, Jd

Environmental and Earth Law Journal (EELJ)

In this lead article, the authors build on the idea that we do not need more environmental law in response to the deteriorating health of the natural world. Rather, they argue that what is needed are different approaches to managing human relationships with the earth. They argue that the burgeoning Earth jurisprudence movement offers a deep philosophical anchor and a range of practical and multi-disciplinary approaches necessary to create law reform and societal change that will better support the natural world and human societies than our current system. The authors will outline the origins and key elements of the Earth …

Optimizing Production Of Fc-Amidated Peptides By Chinese Hamster Ovary Cells, Kristina Carlson, Richard E. Mains, Betty A. Eipper

UCHC Articles - Research

Background

Amidation of the carboxyl terminal of many peptides is essential for full biological potency, often increasing receptor binding and stability. The single enzyme responsible for this reaction is peptidylglycine α-amidating monooxygenase (PAM: EC 1.14.17.3), a copper- and ascorbate-dependent Type I membrane protein.

Methods

To make large amounts of high molecular weight amidated product, Chinese hamster ovary (CHO) cells were engineered to express exogenous PAM. To vary access of the enzyme to its substrate, exogenous PAM was targeted to the endoplasmic reticulum, trans-Golgi network, endosomes and lysosomes or to the lumen of the secretory pathway.

Results

PAM was equally …

Machinal, Jennifer Popple

2010-2019: All Shows

The play's title means "automatic" or "mechanical" in French and is based loosely on the murder trial of Ruth Snyder and her lover, Judd Gray, who together murdered Snyder's husband. Convicted of murdering her husband, Snyder later received the electric chair. A woman's role during this era in history is confined and regimented to wife, mother, housekeeper, and sexual partner. Love is considered unnecessary, and thus many women are trapped in their dependent status, living a hellish life in a loveless marriage. The relationship between Helen Jones and her husband, George H. Jones, is no different. However, when a man …

Supporting Teachers’ Learning About Mathematical Modeling, June L. Gastón, Barbara A. Lawrence

Publications and Research

In the United States, one of the Standards for Mathematical Practice of the Common Core Curriculum (Common Core State Standards Initiative, 2010) is Model with mathematics. This standard requires that students be taught in a manner that will enable them to ―apply the mathematics they know to solve problems arising in everyday life, society, and the workplace‖ (p. 7). However many prospective and practicing teachers acquire a pedagogical style that does not support this standard. To promote higher levels of student thinking associated with mathematical modeling, teachers must thus be taught not only what mathematical modeling is, but how it …

Laterality Enhances Numerical Skills In The Guppy, Poecilia Reticulata, Marco Dadda, Christian Agrillo, Angelo Bisazza, Culum Brown

Sentience Collection

It has been hypothesized that cerebral lateralization can significantly enhance cognition and that this was one of the primary selective forces shaping its wide-spread evolution amongst vertebrate taxa. Here, we tested this hypothesis by examining the link between cerebral lateralization and numerical discrimination. Guppies, Poecilia reticulata, were sorted into left, right and non-lateralized groups using a standard mirror test and their numerical discrimination abilities tested in both natural shoal choice and abstract contexts. Our results show that strongly lateralized guppies have enhanced numerical abilities compared to non-lateralized guppies irrespective of context. These data provide further credence to the notion that …

Autophagy Protects Against Cyp2e1/Chronic Ethanol-Induced Hepatotoxicity, Yongke Lu, Arthur I. Cederbaum

Pharmacology, Physiology and Toxicology

Autophagy is an intracellular pathway by which lysosomes degrade and recycle long-lived proteins and cellular organelles. The effects of ethanol on autophagy are complex but recent studies have shown that autophagy serves a protective function against ethanol-induced liver injury. Autophagy was found to also be protective against CYP2E1-dependent toxicity in vitro in HepG2 cells which express CYP2E1 and in vivo in an acute alcohol/CYPE1-dependent liver injury model. The goal of the current report was to extend the previous in vitro and acute in vivo experiments to a chronic ethanol model to evaluate whether autophagy is also protective against CYP2E1-dependent liver …

Um Green Fund Seeks New Proposals, Joe Scott

University of Mississippi News

Applications for campus sustainability projects accepted through Oct. 19

Board Of Trustees Agenda, Oct. 16 2015, Indiana State University Board Of Trustees

Board of Trustees

No abstract provided.

Antimicrobial Copper Alloy Surfaces Are Effective Against Vegetative But Not Sporulated Cells Of Gram-Positive Bacillus Subtilis, Kaungmyat San, Janet Long, Corinne A. Michels, Nidhi Gadura

Publications and Research

This study explores the role of membrane phospholipid peroxidation in the copper alloy mediated contact killing of Bacillus subtilis, a spore-forming gram-positive bacterial species. We found that B. subtilis endospores exhibited significant resistance to copper alloy surface killing but vegetative cells were highly sensitive to copper surface exposure. Cell death and lipid peroxidation occurred in B. subtilis upon copper alloy surface exposure. In a sporulation-defective strain carrying a deletion of almost the entire SpoIIA operon, lipid peroxidation directly correlated with cell death. Moreover, killing and lipid peroxidation initiated immediately and at a constant rate upon exposure to the copper surface …

Volume Cxxxiii, Number 5, October 16, 2015, Lawrence University

The Lawrentian

No abstract provided.

Reductive Individualism And The Just War Framework

Legal Philosophy between State and Transnationalism Seminar Series

time: 2:30 pm – 4:30 pm
room: Osgoode, IKB 4034
speaker: Helen Frowe (Stockholm)

An Unattended Mask Makes An Attended Target Disappear, Arielle A. Veenemans, Patrick Cavanagh

Dartmouth Scholarship

In pattern masking, the target and mask are presented at the same location and follow one another very closely in time. When the observer attends to the target, he or she must also attend to the mask, as the switching time for attention is quite slow. In a series of experiments, we present mask–target–mask sequences staggered in time and location (Cavanagh, Holcombe, & Chou, 2008) that allow participants to attentively track the target location without attending to the masks. The results show that the strength of masking is on average unaffected by the removal of attention from the masks. Moreover, …

Minutes, University Of South Florida St. Petersburg General Education Committee Meeting, October 16, 2015, University Of South Florida St. Petersburg. Faculty Senate. General Education Committee

USF St. Petersburg General Education Committee Meetings

Minutes from the October 16, 2015, meeting of the General Education Committee of the University of South Florida St. Petersburg's Faculty Senate.

Burkholderia Pseudomallei Capsular Polysaccharide Recognition By A Monoclonal Antibody Reveals Key Details Toward A Biodefense Vaccine And Diagnostics Against Melioidosis, Roberta Marchetti, Michael J. Dillon, Mary N. Burtnick, Mark A. Hubbard, Marielle Tamigney Kenfack, Yves Blériot, Charles Gauthier, Paul J. Brett, David P. Aucoin, Rosa Lanzetta, Alba Silipo, Antonio Molinaro

Peninsula Medical School

No abstract provided.

Bible Stories No One Talks About, Mark Verbruggen

Faculty Work Comprehensive List

"These are real people, messed up and sinful people, and yet they are the ones used by God to advance his Kingdom of grace and truth in Jesus Christ."

Posting about dealing with ­­­­­­­­the unpleasant narratives in the Bible from In All Things - an online hub committed to the claim that the life, death, and resurrection of Jesus Christ has implications for the entire world.

http://inallthings.org/bible-stories-no-one-talks-about/

October 16, 2015, The Dakota Student

The Dakota Student

No abstract provided.

Genestation 1.0: A Synthetic Resource Of Diverse Evolutionary And Functional Genomic Data For Studying The Evolution Of Pregnancy-Associated Tissues And Phenotypes, Mara Kim, Brian A. Cooper, Rohit Venkat, Julie B. Phillips, Haley R. Eidem, Jibril Hirbo, Sashank Nutakki, Scott M. Williams, Louis J. Muglia, J. Anthony Capra, Kenneth Petren, Patrick Abbot, Antonis Rokas, Kriston L. Mcgary

Dartmouth Scholarship

Mammalian gestation and pregnancy are fast evolving processes that involve the interaction of the fetal, maternal and paternal genomes. Version 1.0 of the GEneSTATION database (http://genestation.org) integrates diverse types of omics data across mammals to advance understanding of the genetic basis of gestation and pregnancy-associated phenotypes and to accelerate the translation of discoveries from model organisms to humans. GEneSTATION is built using tools from the Generic Model Organism Database project, including the biology-aware database CHADO, new tools for rapid data integration, and algorithms that streamline synthesis and user access. GEneSTATION contains curated life history information on pregnancy and …

Synthesis Of New 4-Aminoquinolines And Evaluation Of Their In Vitro Activity Against Chloroquine-Sensitive And Chloroquine-Resistant Plasmodium Falciparum, Chandima S. K. Rajapakse, Maryna Lisai, Christiane Deregnaucourt, Véronique Sinou, Christine Latour, Dipankar Roy, Joseph Schrével, Roberto A. Sánchez-Delgado

Publications and Research

The efficacy of chloroquine, once the drug of choice in the fight against Plasmodium falciparum, is now severely limited due to widespread resistance. Amodiaquine is one of the most potent antimalarial 4-aminoquinolines known and remains effective against chloroquine-resistant parasites, but toxicity issues linked to a quinone-imine metabolite limit its clinical use. In search of new compounds able to retain the antimalarial activity of amodiaquine while circumventing quinone-imine metabolite toxicity, we have synthesized five 4-aminoquinolines that feature rings lacking hydroxyl groups in the side chain of the molecules and are thus incapable of generating toxic quinone-imines. The new compounds displayed high …