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Zhx2 Enhances The Cytotoxicity Of Chemotherapeutic Drugs In Liver Tumor Cells By Repressing Mdr1 Via Interfering With Nf-Ya, Hongxin Ma, Xuetian Yue, Lifen Gao, Xiaohong Liang, Wenjiang Yan, Zhenyu Zhang, Haixia Shan, Hualin Zhang, Brett T. Spear, Chunhong Ma

Microbiology, Immunology, and Molecular Genetics Faculty Publications

We previously reported the tumor suppressor function of Zinc-fingers and homeoboxes 2 (ZHX2) in hepatocellular carcinoma (HCC). Other studies indicate the association of increased ZHX2 expression with improved response to high dose chemotherapy in multiple myeloma. Here, we aim to test whether increased ZHX2 levels in HCC cells repress multidrug resistance 1(MDR1) expression resulting in increased sensitivity to chemotherapeutic drugs. We showed evidence that increased ZHX2 levels correlated with reduced MDR1 expression and enhanced the cytotoxicity of CDDP and ADM in different HCC cell lines. Consistently, elevated ZHX2 significantly reduced ADM efflux in HepG2 cells and greatly increased the CDDP-mediated …

Pyruvate Carboxylase Is Critical For Non-Small-Cell Lung Cancer Proliferation, Katherine Sellers, Matthew P. Fox, Michael Bousamra Ii, Stephen P. Slone, Richard M. Higashi, Donald M. Miller, Yali Wang, Jun Yan, Mariia O. Yuneva, Rahul Deshpande, Andrew N. Lane, Teresa W-M Fan

Toxicology and Cancer Biology Faculty Publications

Anabolic biosynthesis requires precursors supplied by the Krebs cycle, which in turn requires anaplerosis to replenish precursor intermediates. The major anaplerotic sources are pyruvate and glutamine, which require the activity of pyruvate carboxylase (PC) and glutaminase 1 (GLS1), respectively. Due to their rapid proliferation, cancer cells have increased anabolic and energy demands; however, different cancer cell types exhibit differential requirements for PC- and GLS-mediated pathways for anaplerosis and cell proliferation. Here, we infused patients with early-stage non-small-cell lung cancer (NSCLC) with uniformly 13C-labeled glucose before tissue resection and determined that the cancerous tissues in these patients had enhanced PC activity. …

Method For Determining The Origin Of A Sample, John Ballantyne, Erin Hanson, Helge Lubenow

UCF Patents

A method for determining the nature of a sample is provided, wherein the presence or absence of at least one marker small non-coding RNA in the sample is detected. Suitable marker small non-coding RNAs for different samples such as blood, saliva, semen and vaginal secretions are provided. Also provided are suitable kits and methods for identifying marker small-non coding RNAs.

Cyborg Beast: A Low-Cost 3d-Printed Prosthetic Hand For Children With Upper-Limb Differences, Jorge Zuniga, Dimitrios Kastavelis, Jean Peck, John Stollberg, Marc Petrykowski, Adam Carson, Cristina Fernandez

Journal Articles

Background

There is an increasing number of children with traumatic and congenital hand amputations or reductions. Children's prosthetic needs are complex due to their small size, constant growth, and psychosocial development. Families’ financial resources play a crucial role in the prescription of prostheses for their children, especially when private insurance and public funding are insufficient. Electric-powered (i.e., myoelectric) and body-powered (i.e., mechanical) devices have been developed to accommodate children’s needs, but the cost of maintenance and replacement represents an obstacle for many families. Due to the complexity and high cost of these prosthetic hands, they are not accessible to children …

The Bg News January 20, 2015, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. January 20, 2015. Volume 94-Issue 54.

Emily Lukasiak's Experience Second To None At Unh Manchester, Unh Communications And Public Affairs

UNH Today Archive

No abstract provided.

Search Committee Named, University Of Dayton

News Releases

The executive committee of the University of Dayton's board of trustees has appointed alumnus and trustee David P. Yeager as chair of the presidential search committee.

Horticulturae — An International, Multidisciplinary, Open Access Journal, Douglas D. Archbold

Horticulture Faculty Publications

No abstract provided.

The George-Anne, Georgia Southern University

The George-Anne

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Cliffdwellers: A Study In Wildness, Stoney Larkin Sasser

Theses and Dissertations

How do I find wildness? This question is a central tenant of my work and manifests psychologically and physically in my installation, video and performance work. As the diversity of non-human ecosystems wanes and wildness along with it, I am overwhelmed by idea that the human generated debris might eventually cover the earth and become our new landscape, our new wilderness. I wonder "what do we do with all of this stuff'? In my installations, I play with the materials I gather and accumulate. I construct `ecosystems' often using pedestrian materials such as nylons, fabric, lint and glitter to explore …

Aggregation And Satiation: Finding Meaning In Repetition, Samantha Buchanan

Theses and Dissertations

In my work, I see repetition, but also complexity and variation. This supportive

statement examines my interest in the aggregate, in forms composed of multiples, as well as the repetitive processes required in their construction. I look at the ways in which predictability, recursion, and rhythm function in my repetitive practice, as well as how the energy expended throughout the process manifests in the matter of the work. What I intend for the viewer is the perception of a holistic and simultaneous sensory experience.

An Analysis Of Cia And Military Testing Of Lsd On Non-Consenting U.S. Service Members And Recovery Through The Va Disability System, B.M. Disbennett

Tennessee Journal of Race, Gender, & Social Justice

No abstract provided.

Surrogate Or “Mother”? The Problem Of Traditional Surrogacy, Frank J. Bewkes

Tennessee Journal of Race, Gender, & Social Justice

No abstract provided.

Moderating Mayo, Bernard Chao

Northwestern University Law Review

No abstract provided.

Cseas Weekly Bulletin (January 19, 2015), Center For Southeast Asian Studies

CSEAS Bulletin

No abstract provided.

Current, January 19, 2015, University Of Missouri-St. Louis

Current (2010s)

No abstract provided.

High Ph Microbial Ecosystems In A Newly Discovered, Ephemeral, Serpentinizing Fluid Seep At Yanartaş (Chimera), Turkey, D'Arcy R. Meyer-Dombard, Kristin M. Woycheese, Erin N. Yargıçoğlu, Dawn Cardace, Yasemin Güleçal-Pektas, Mustafa Temel

Geosciences Faculty Publications

Gas seeps emanating from Yanartaş (Chimera), Turkey, have been documented for thousands of years. Active serpentinization produces hydrogen and a range of carbon gases that may provide fuel for life. Here we report a newly discovered, ephemeral fluid seep emanating from a small gas vent at Yanartaş. Fluids and biofilms were sampled at the source and points downstream. We describe site conditions, and provide microbiological data in the form of enrichment cultures, Scanning electron microscopy (SEM), carbon and nitrogen isotopic composition of solids, and PCR screens of nitrogen cycle genes. Source fluids are pH 11.95, with a Ca:Mg of ~200, …

Grand Valley Forum, Volume 039, Number 17, January 19, 2015, Grand Valley State University

2014-2015, Volume 39

Grand Valley Forum is Grand Valley State's faculty and staff newsletter, published from 1976 to the present.

Elephant (Elephas Maximus) Health And Management In Asia: Variations In Veterinary Perspectives, David Miller, Bradford Jackson, Heidi S. Riddle, Christopher Stremme, Dennis Schmitt, Thaddeus Miller

College of Agriculture

There is a need to identify strategic investments in Asian elephant (Elephas maximus) health that will yield maximal benefits for overall elephant health and conservation. As an exploratory first step, a survey was administered to veterinarians from Asian elephant range countries at a workshop and via email to help prioritize health-related concerns that will mostly benefit elephants. Responses were received from 45 veterinarians from eight countries that had a range of experience with captive and wild elephants. The occurrence of medical conditions and responses to treatment varied among responses. However, injuries, parasitism, and gastrointestinal disease were reported as the most …

Effects Of (R)-(-)-5-Methyl-1-Nicotinoyl-2-Pyrazoline On Glutamate Transporter 1 And Cysteine/Glutamate Exchanger As Well As Ethanol Drinking Behavior In Male, Alcohol-Preferring Rats, Munaf Aal-Aaboda, Hasan Alhaddad, Francis Osowik, Surya M. Nauli, Youssef Sari

Pharmacy Faculty Articles and Research

Alcohol consumption is largely associated with alterations in the extracellular glutamate concentrations in several brain reward regions. We recently showed that glutamate transporter 1 (GLT-1) is downregulated following chronic exposure to ethanol for 5 weeks in alcohol-preferring (P) rats and that upregulation of the GLT-1 levels in nucleus accumbens and prefrontal cortex results, in part, in attenuating ethanol consumption. Cystine glutamate antiporter (xCT) is also downregulated after chronic ethanol exposure in P rats, and its upregulation could be valuable in attenuating ethanol drinking. This study examines the effect of a synthetic compound, (R)-(−)-5-methyl-1-nicotinoyl-2-pyrazoline (MS-153), on ethanol drinking and expressions of …

The Corporation As Time Machine: Intergenerational Equity, Intergenerational Efficiency, And The Corporate Form, Lynn A. Stout

Seattle University Law Review

This Symposium Article argues that the board-controlled corporation can be understood as a legal innovation that historically has functioned as a means of transferring wealth forward and sometimes backward through time, for the benefit of present and future generations. In this fashion the board-controlled corporation promotes both intergenerational equity and intergenerational efficiency. Logic and evidence each suggest, however, that the modern embrace of “shareholder value” as the only corporate objective and “shareholder democracy” as the ideal of corporate governance is damaging the corporate form’s ability to serve this economically and ethically important function.

The Collegian (2015-01-19), Jesus Sanchez

UTB/TSC Archives - The Collegian [Newspaper]

Vol. 67, No. 16

Usf St. Petersburg Striving To Reduce Carbon Footprint, Eillin Delpaz, Wusf Public Media

Student Green Energy Fund (SGEF) Water Refill Stations

An article about USF St. Petersburg's Climate Action Plan enacted in 2016 to reduce greenhouse gas emisions by 50% by 2035 and ultimately achieve carbon neutrality by 2050.

The Real State Of The Union 2015, Lauren Carasik

Media Presence

No abstract provided.

Altered Γ-Aminobutyric Acid Neurotransmission In Major Depressive Disorder: A Critical Review Of The Supporting Evidence And The Influence Of Serotonergic Antidepressants, Alan Pehrson, Connie Sanchez

Department of Psychology Faculty Scholarship and Creative Works

Evidence suggesting that central nervous system γ-aminobutyric acid (GABA) concentrations are reduced in patients with major depressive disorder (MDD) has been present since at least 1980, and this idea has recently gained support from more recent magnetic resonance spectroscopy data. These observations have led to the assumption that MDD’s underlying etiology is tied to an overall reduction in GABA-mediated inhibitory neurotransmission. In this paper, we review the mechanisms that govern GABA and glutamate concentrations in the brain, and provide a comprehensive and critical evaluation of the clinical data supporting reduced GABA neurotransmission in MDD. This review includes an evaluation of …

Oxabicyclo[3.2.1]Octadienes As Building Blocks In The Synthesis Of Natural Products, Michael D. Vanheyst

Doctoral Dissertations

Since the initial report by Diels and Alder in 1928, the [4+2] cycloaddition remains one of the most powerful and most frequently employed methods for the construction of six membered ring systems, generating a high degree of structural complexity in a single synthetic transformation. This cycloaddition process not only generates two new σ-bonds, but also establishes up to four new contiguous stereocenters in the process, owing to the high regio- and stereoselectivities displayed by this pericyclic reaction. In the decades following its discovery, Tobey and Law would report on the intriguing reaction of furan, substituted furans, and cyclopentadiene with both …

Highly Constrained Intergenic Drosophila Ultraconserved Elements Are Candidate Ncrnas, Andrew D. Kern, Daniel A. Barbash, Joshua Chang Mell, Daniel Hupalo, Amanda Jensen

Dartmouth Scholarship

Eukaryotes contain short (∼80–200 bp) regions that have few or no substitutions among species that represent hundreds of millions of years of evolutionary divergence. These ultraconserved elements (UCEs) are candidates for containing essential functions, but their biological roles remain largely unknown. Here, we report the discovery and characterization of UCEs from 12 sequenced Drosophilaspecies. We identified 98 elements ≥80 bp long with very high conservation across the Drosophila phylogeny. Population genetic analyses reveal that these UCEs are not present in mutational cold spots. Instead we infer that they experience a level of selective constraint almost 10-fold higher compared with …

Suppressed Autophagy Flux In Skeletal Muscle Of An Amyotrophic Lateral Sclerosis Mouse Model During Disease Progression, Yajuan Xiao, Changling Ma, Jianxun Yi, Shaoping Wu, Guo Luo, Xiulong Xu, Peihui Lin, Jun Sun, Jingsong Zhou

Faculty and Staff Publications

Accumulation of abnormal protein inclusions is implicated in motor neuron degeneration in amyotrophic lateral sclerosis (ALS). Autophagy, an intracellular process targeting misfolded proteins and damaged organelles for lysosomal degradation, plays crucial roles in survival and diseased conditions. Efforts were made to understand the role of autophagy in motor neuron degeneration and to target autophagy in motor neuron for ALS treatment. However, results were quite contradictory. Possible autophagy defects in other cell types may also complicate the results. Here, we examined autophagy activity in skeletal muscle of an ALS mouse model G93A. Through overexpression of a fluorescent protein LC3-RFP, we found …

Long-Range Pcr Amplification Of Dna By Dna Polymerase Iii Holoenzyme From Thermus Thermophilus, Wendy Ribble, Shawn D. Kane, James M. Bullard

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

DNA replication in bacteria is accomplished by a multicomponent replicase, the DNA polymerase III holoenzyme (pol III HE). The three essential components of the pol III HE are the α polymerase, the β sliding clamp processivity factor, and the DnaX clamp-loader complex. We report here the assembly of the functional holoenzyme from Thermus thermophilus (Tth), an extreme thermophile. The minimal holoenzyme capable of DNA synthesis consists of α, β and DnaX ( and γ), and components of the clamp-loader complex. The proteins were each cloned and expressed in a native form. Each component of the system was purified extensively. The …

Medical School Newsletter - January 18, 2015, Lindsey Lyle

Watercooler Newsletter

This is the January 18, 2015 edition of the Frederick P. Whiddon College of Medicine's Newsletter - Watercooler.

Contents Include:

Mark Your Calendar: Autism Grand Rounds

Mobile County Public School System Airs Med School Café Lectures

Medical Student Eyes Ophthalmology as Career Path

Jan. 22 DSS to Feature Dr. Raymond Langley