Digital Commons Network^™

Genetic And Structural Analyses Of Poliovirus Protein 2catpase, Emmanuel Noble Asare

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

The specificity of encapsidation of members of the genus Enterovirus of the Picornaviridae family depends on an interaction between capsid proteins and nonstructural protein 2CATPase. In particular, in the context of a chimeric virus, the N252 residue of poliovirus (PV) 2CATPase interacts with VP3 E180 of coxsackievirus A20. PV 2CATPase has important roles both in RNA replication and encapsidation, but in the context of just the poliovirus polyprotein, N252 does not function in assembly. The aim of my study was to search for additional residues near N252 in 2CATPase that are required for PV encapsidation. I predicted a three-dimensional protein …

Progression Magazine, 2015 Winter, Coastal Carolina University

Progression Magazine

Magazine of the College of Science at Coastal Carolina University.

Gopher Intrusion In Cropping Systems, Kenneth Romero, Paul Cardinalli

Agricultural Education and Communication

The purpose of this project was to determine if no till vs. till on gopher populations was more beneficial. Which is more efficient at controlling gophers and explain the damage they can cause and the diseases they can potentially vector. What are the different options of pest managements that have to be used when dealing with a gopher infestation in a tilled field vs. a no-tilled field.

Mechanism Of Type Iii Secretion System-Triggered Killing Of Yersinia In Macrophages, Xiaoying Wang

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

The mammalian immune system has the ability to discriminate between pathogens and innocuous microbes by detecting conserved molecular patterns. In addition to conserved microbial patterns, the mammalian immune system may recognize distinct pathogen-induced processes, the mechanism of which is poorly understood. Pathogenic Yersinia species utilize a type III secretion system (T3SS) to translocate various bacterial effectors into target cells, which aim to modify multiple host signaling pathways. Interestingly, previous studies have shown that the T3SS in Yersinia pseudotuberculosis leads to decreased survival of this bacterium in primary murine macrophages, the mechanism of which is unknown. Here, we use colony forming …

On Using Risk-Benefit Analysis To Assess And Manage Controversial Research, Daniel Rozell

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Science research in the twenty-first century increasingly results in powerful and rapidly disseminated technologies that raise concerns over technological risk and public safety. A common recommendation for assessing and managing controversial research is to perform a quantitative risk-benefit analysis (RBA) in order to help determine an appropriate course of action. However, these recommendations lack guidelines on how to perform such an assessment. This dissertation lays out a detailed argument why a quantitative RBA should not be used as a basis for making complex policy decisions. Rather, RBA should be regarded as a tool for exploring the impacts of technology on …

Demography And Disease Of The Rare Shrub Buckleya Distichophylla (Santalaceae) In Northeastern Tennessee, William Seth Ratliff

Undergraduate Honors Theses

Piratebush (Buckleya distichophylla (Nutt.) Torr.) is a rare, hemiparasitic shrub with the only extant populations in western North Carolina, northeastern Tennessee, and southwestern Virginia. The preferred natural hosts of piratebush, Carolina and eastern hemlocks, have seen sharp declines over the last decade due to the invasive hemlock woolly adelgid. Virginia pine, another important host of piratebush, is also susceptible to disease, specifically Cronartium appalachianum, a rust fungus for which piratebush is the secondary host. This study described and analyzed current demographic parameters of three Tennessee piratebush populations. Additionally, spatial patterns of disease and demographic characters were analyzed. These …

An Examination Of Microbial Community Composition At Tba-Degrading Versus Non-Degrading Sites, Courtney Thompson

All Theses

Understanding how fuel oxygenates interact with microorganisms in the subsurface is important for the remediation of contaminated sites and designing and optimizing remedial strategies. The current understanding of methyl tertiary butyl ether (MTBE) and tertiary butyl alcohol (TBA) degradation in the environment has been developed primarily through gas chromatography GC analysis and culture-based laboratory studies. Microbial communities can be very diverse, and while microcosm studies provide valuable data, few metagenomic studies have been performed. To elucidate the significant microorganisms and pathways involved in the breakdown of MTBE and TBA, the compositions of entire indigenous microbial populations must be investigated and …

Structural Insights Into Mitochondrial Gene Expression And Disease, Kip Guja

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Mitochondria are dual membrane-bound organelles found in the cytoplasm of all eukaryotic cells. Human mitochondria contain a 16.5 kb double-stranded DNA genome that encodes thirteen essential protein subunits of the oxidative phosphorylation system, as well as the two ribosomal RNAs and 22 transfer RNAs needed to translate the thirteen messenger RNAs. Oxidative phosphorylation produces the majority of energy needed for cells to sustain life, and this process is strictly dependent on expression of the mitochondrial genome. Defects in mitochondrial gene expression are linked to many human diseases, age-related pathologies, and the aging process itself. Obtaining a more detailed understanding of …

Modified Low-Density Lipoprotein From Mycobacterium Tuberculosis Induces Foamy Macrophage Formation, Guannan Chen

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Tuberculosis (TB) is one of the major causes of mortality in the world, causing approximately 1.5 million deaths every year, among which children take a great part. However, due to the perception that children are rarely infectious or they rarely develop severe disease, children with TB have always been neglected. What is worse, diagnosis of childhood TB is difficult, making it harder to control the disease. Foamy macrophages are a key component in the pathology of TB, and the formation of foamy macrophages foresees the progression of infection to active disease. Low-density lipoprotein (LDL) is one of the major lipoproteins …

Chemoattractant Receptors Blt1 And Cxcr3 Regulate Anti-Tumor Immunity By Facilitating Cd8+ T Cell Migration To Tumors., Zinal S. Chheda

Electronic Theses and Dissertations

Presence of increased numbers of CD8⁺ T cells in the tumors correspond to better overall survival in the patients. Variety of immuno-therapies have shown considerable efficacy in the clinic, however, a multitude of patients remain unresponsive. Most of these immunotherapies rely on effector T cell responses in the tumor. A major obstacle in the success of these immunotherapies is poor recruitment of CD8⁺ T cells into tumors despite intact effector responses in the periphery. Therefore understanding the mechanisms that regulate CTL infiltration into tumors becomes essential. Previous studies in our laboratory suggested an important role for BLT1 in …

Synthesis And Functional Evaluation Of Peptide Modified Poly (Lactic-Co-Glycolic Acid) Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., Paridhi Kalia

Electronic Theses and Dissertations

Periodontal disease is an oral inflammatory disorder that afflicts roughly 46% of the adults in the U.S. Currently, treatment of periodontal disease involves the removal of plaque from the gingival pocket (with possible antibiotic treatment) and if necessary, gingival surgery. To our knowledge, no therapeutic approach exists that promotes host-biofilm homeostasis by limiting pathogen recolonization of the oral cavity after prophylaxis or treatment. The interaction of the pathogen Porphyromonas gingivalis with commensal streptococci is critical for initiation of periodontitis and represents a target for limiting P. gingivalis colonization of the oral cavity. Previous studies showed that a synthetic peptide …

December 2015 News Releases, University Of Montana--Missoula. Office Of University Relations

University of Montana News Releases, 1928, 1956-present

No abstract provided.

Forage News [2015-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• New Seed Coating for Alfalfa
• Forage News Quote of the Month
• *New for Forage News* - Featured Publication: Managing Diseases of Alfalfa
• Successful International Grassland Congress
• Tall Fescue in the 21st Century is the Focus for the 2016 Virginia Winter Forage Conferences
• Heart of America Registration Open
• UK Ag Equine Programs to Host Equine Showcase, Breeder's Short Course
• Heart of America Grazing Conference Registration Form

The Global Architecture Of Financial Regulatory Taxes, Carlo Garbarino, Giulio Allevato

Michigan Journal of International Law

This Article endeavors to broaden the analysis of available policy tools to address the problems created by financial crises and discusses how, in addition to direct regulation, certain tax measures having a regulatory nature may operate to address the so-called “negative externalities” often associated with those crises. There is a negative externality when an economic agent making a decision does not pay the full cost of the decision’s consequences. In such cases, the cost to society as a whole is greater than the cost borne by the individuals creating the economic impact. In practice, negative externalities result in market inefficiencies …

Abctools: An R Package For Tuning Approximate Bayesian Computation Analyses, Matthew A. Nunes, Dennis Prangle

The R Journal

Approximate Bayesian computation (ABC) is a popular family of algorithms which perform approximate parameter inference when numerical evaluation of the likelihood function is not possible but data can be simulated from the model. They return a sample of parameter values which produce simulations close to the observed dataset. A standard approach is to reduce the simulated and observed datasets to vectors of summary statistics and accept when the difference between these is below a specified threshold. ABC can also be adapted to perform model choice.

In this article, we present a new software package for R, abctools which provides methods …

Age Differences In Loneliness: Neural Correlates, Neurogenetics And Functional Connectivity, Alexandra D'Agostino

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Loneliness, defined as the subjective experience of social isolation, has been linked to poor health outcomes (e.g. depression, cardiovascular disease) and is prevalent among older adult populations. To improve upon current treatments, the interaction between genetic and environmental risk factors for loneliness and their effect on the brain must be better understood. The present study investigated the brain basis of loneliness in younger (mean age = 20.4) and older adults (mean age = 62.9). We used functional magnetic resonance imaging (fMRI) and an emotional picture task to address this question. fMRI data were collected on a 3T Siemens Trio Scanner, …

A Review Of 'Body Farm' Research Facilities Across America With A Focus On Policy And The Impacts When Dealing With Decompositional Changes In Human Remains, Brittany M. Wolff

Criminology & Criminal Justice Theses - Archive

In the current research study, the use of content analysis and secondary data analysis provided the exploratory research on the “body farm” research facilities across the United States. Open-ended survey questions were also utilized and sent to the six research facilities across the country. The use of open-ended survey questions in combination with a small sample size, resulted in only one institution participating in the survey and one facility actively declining to participate. To resolve this limitation, the use of secondary data and content analysis from facility publications and websites were utilized to complete some of the gaps resulting from …

Natural Degradation Of Earthworks, Trenches, Walls And Moats, Northern Thailand, Spencer H. Wood, Layle R. Wood, Alan D. Ziegler

Geosciences Faculty Publications and Presentations

“………..structures of this kind are hidden away securely under the thick overgrowth: thus does nature preserve what man would surely destroy” (from Sumet Jumsai, 1970)

We investigate the geometry, age, and history of several enigmatic northern Thailand earthwork entrenchments that are mostly located on hills and could not have held water to form moats. The earthworks are either oval or rectangular in map view; and they typically encircle 0.3-to-1-km² areas that do not have potsherd debris indicative of former towns. Most trenches are 3-5 m deep with inner walls 4.5-8 m high. Some encircling earthworks are concentric double trenches …

Donor Platelet Plasma Components Inactivate Sensitive And Multidrug Resistant Acinetobacter Baumannii Isolates, Chelsea M. Edelblute, Olga N. Pakhomova, Fanying Li, Barbara Y. Hargrave, Loree C. Heller

Bioelectrics Publications

Acinetobacter baumannii is an environmentally resilient healthcare-associated opportunistic pathogen responsible for infections at many body sites. In the last 10 years, clinical strains resistant to many or all commonly used antibiotics have emerged globally. With few antimicrobial agents in the pharmaceutical pipeline, new and alternative agents are essential. Platelets secrete a large number of proteins, including proteins with antimicrobial activity. In a previous study, we demonstrated that donor platelet supernatants and plasma significantly inhibited the growth of a reference strain of A. baumannii in broth and on skin. This inhibition appeared to be unrelated to the platelet activation state. In …

Paratransgenic Control Of Pierce's Disease, Arinder K. Arora

Biology ETDs

Pierce's Disease (PD) is a deadly grapevine disease, which is caused by a Gram negative bacterium, Xylella fastidiosa and transmitted by leafhoppers commonly known as sharpshooters, including the glassy-winged sharpshooter (GWSS, Homalodisca vitripennis). We developed paratransgenic control of PD using a commensal bacterium Pantoea agglomerans E325. The GWSSs acquired enhanced green fluorescent (EGFP)-expressing P. agglomerans from an artificial feeding system and the bacteria persisted within the insect foregut for two weeks. P. agglomerans was selected as the paratransgenic control agent based on its persistence within the sharpshooter foregut, a niche also inhabited by X. fastidiosa. P. agglomerans lines secreting two …

Mathematics Library News 14, Aaron Lercher

Faculty Publications

No abstract provided.

Failure Of A Heterologous Recombinant Sca5/Ompb Protein-Based Vaccine To Elicit Effective Protective Immunity Against Rickettsia Rickettsii Infections In C3h/Hen Mice, Sean P. Riley, Marissa M. Cardwell, Yvonne G. Chan, Ludovic Pruneau, Fabio Del Piero, Juan J. Martinez

Faculty Publications

Spotted fever group (SFG) rickettsial species are obligate intracellular tick-borne pathogens that are responsible for important human diseases. Previous reports have demonstrated the feasibility of using recombinant surface cell antigen Sca5/OmpB to elicit protective immunity against homologous challenges using murine models of Mediterranean spotted fever and Rocky Mountain spotted fever. In addition, the feasibility of generating cross-protective immunity against related rickettsial species has also been established, but the molecular basis for these phenomena was not explored. Here, we demonstrate that vaccination of C3H/HeN mice with a recombinant OmpB domain derived from Rickettsia conorii induced high titer humoral immune responses that …

Emergency Takings, Brian Lee

Faculty Scholarship

No abstract provided.

Utilizing Monte Carlo Simulations To Optimize Institutional Empiric Antipseudomonal Therapy, Sarah J. Tennant, Donna R. Burgess, Jeffrey M. Rybak, Craig A. Martin, David S. Burgess

Pharmacy Practice and Science Faculty Publications

Pseudomonas aeruginosa is a common pathogen implicated in nosocomial infections with increasing resistance to a limited arsenal of antibiotics. Monte Carlo simulation provides antimicrobial stewardship teams with an additional tool to guide empiric therapy. We modeled empiric therapies with antipseudomonal β-lactam antibiotic regimens to determine which were most likely to achieve probability of target attainment (PTA) of ≥90%. Microbiological data for P. aeruginosa was reviewed for 2012. Antibiotics modeled for intermittent and prolonged infusion were aztreonam, cefepime, meropenem, and piperacillin/tazobactam. Using minimum inhibitory concentrations (MICs) from institution-specific isolates, and pharmacokinetic and pharmacodynamic parameters from previously published studies, a 10,000-subject Monte …

Decimation, Rejuvenation, Motivation: How Disease And Murder Set The Stage At Saratoga, Nicolas Soto

History

No abstract provided.

The Commander In Chief's Authority To Combat Climate Change, Mark P. Nevitt

Cardozo Law Review

Climate change is the world's greatest environmental threat. It is also increasingly understood as a threat to domestic and international peace and security. In recognition of this threat, the President has taken the initiative to prepare for climate change's impact - in some cases drawing sharp objections from Congress. While both the President and Congress have certain constitutional authorities to address the national security threat posed by climate change, the precise contours of their overlapping powers are unclear. As Commander in Chief, the President has the constitutional authority to repel sudden attacks and take care that the laws are faithfully …

High Prevalence And Genetic Heterogeneity Of Rodent-Borne Bartonella Species On Heixiazi Island, China, Dong-Mei Li, Yong Hou, Xiu-Ping Song, Ying-Qun Fu, Gui-Chang Li, Ming Li, Marina E. Eremeeva, Hai-Xia Wu, Bo Pang, Yu-Juan Yue, Ying Huang, Liang Lu, Jun Wang, Qi-Yong Liu

Environmental Health Sciences: Faculty Publications

We performed genetic analysis of Bartonella isolates from rodent populations from Heixiazi Island in northeast China. Animals were captured at four sites representing grassland and brushwood habitats in 2011 and examined for the prevalence and genetic diversity of Bartonella species, their relationship to their hosts, and geographic distribution. A high prevalence (57.7%) and a high diversity (14 unique genotypes which belonged to 8 clades) of Bartonella spp. were detected from 71 rodents comprising 5 species and 4 genera from 3 rodent families. Forty-one Bartonella isolates were recovered and identified, including B. taylorii, B. japonica, B. coopersplainsensis, B. grahamii, B. washoensis …

A Community-Based Participatory Approach To Understanding Health Beliefs, Policies, Barriers, And Solutions Related To The Health Disparities Of Marshallese Cofa Migrants In Arkansas., Pearl Anna Mcelfish

Graduate Theses and Dissertations

Public policies have different effects on different populations groups and can perpetuate health disparities among some populations. My research utilizes community-based participatory approach to research in the examination of one unique population: the Marshallese. This dissertation research presents three papers that are part of a cohesive research agenda predicated on community-based participatory research (CBPR) to facilitate policy-oriented learning. My research can be used to inform health policy, health care services, and health education. Chapter Two presents the article titled: Health Beliefs of US Marshallese Regarding Type 2 Diabetes.

This article explores the research question: what health beliefs related to diabetes …

Genetic Factors Influencing B-Type Natriuretic Peptide-Mediated Production Of Cyclic Guanosine Monophosphate And Blood Pressure Effects In Heart Failure Patients, David E. Lanfear, Jia Li, Raza Abbas, Ruicong She, Badri Padhukasahasram, Ramesh C. Gupta, David Langholz, W Hw Tang, Keoki L. Williams, Hani N. Sabbah, Sheryl L. Chow

Cardiology Articles

Natriuretic peptides (NPs) represent a critical pathway in heart failure (HF). We explored genetic determinants of pharmacodynamic effects of B-type NP (BNP) and changes in plasma cyclic guanosine monophosphate (cGMP) and blood pressure (BP). HF patients (n = 135) received recombinant human BNP (nesiritide) at standard doses, and plasma cGMP levels were measured at baseline and during infusion. We tested the association of 119 single nucleotide polymorphisms (SNPs) in 4 candidate genes (NPR1, NPR2, NPR3, and membrane metallo-endopeptidase (MME)) with the change in cGMP and BP. Gene-based testing for association of genetic variation with endpoints was significant only for MME. …

Needs Assessment For A Patient Centered Medical Home Model Of Care At The Providence Alaska Cancer Center, Jeremy Rosiecki

Student Projects for Graduate Degrees

In order to better understand the needs of cancer patients and allocate resources, the Providence Alaska Cancer Center requested a needs assessment for an oncology focused patient centered medical home (PCMH). A PCMH allows for coordinated and comprehensive care through the use of a teamwork model that centers on the primary care physician. The Providence Alaska Cancer Center staff randomly selected the records of 200 cancer patients between 2010 and 2011, using the cancer tumor registry. Data were analyzed to answer four specific questions that addressed the 1) presence of a Primary Care Physician (PCP), 2) number and type of …