Digital Commons Network^™

Selection And Use Of Pantoea Dispersa Strain Jfs As A Non-Pathogenic Surrogate For Salmonella Typhimurium Phage Type 42 In Flour, James R. Fudge

Theses and Dissertations

Salmonella, a common food pathogen, costs more than any other pathogen in the United States in terms of health care costs and loss of work due to the illnesses it causes. Low-moisture foods, especially flour, are susceptible to being contaminated by Salmonella. Food producers want flour to be pathogen-free but to also retain the same functionality of non-treated flour. Heat treatment is the most common method employed for lowering the concentration of pathogens in food. However, heating can result in the loss of the flour’s functionality. Pantoea dispersa strain JFS has been isolated from flour as a nonpathogenic bacterial surrogate …

Effects Of Human Maternal Placentophagy On Maternal Postpartum Iron-Status, Laura Kathleen Gryder

UNLV Theses, Dissertations, Professional Papers, and Capstones

Recently, human maternal placenta ingestion, known as placentophagy, has emerged as a rare but growing practice among postpartum mothers in industrialized societies, and is currently found in both home birth and hospital birth settings. The practice is purported to result in certain health benefits for postpartum mothers, some of which could be related to the iron content in full-term placenta (e.g., increased energy and an improved and more rapid postpartum recovery, among others). The aim of this research project was to investigate the effect of encapsulated placentophagy on maternal postpartum iron status via a randomized, double-blind, placebo-controlled pilot study (n=28). …

Identification Of Genes Required To Synthesize An Antibiotic-Like Compound From The Soil Bacterium Rhodococcus Sp. Mtm3w5.2, Amber L. Ward

Electronic Theses and Dissertations

Rhodococcus is a soil bacterium, member of the Actinobacteria, and a close relative of the prolific small molecule producer Streptomyces. Recent interest in Rhodococcus as an under investigated source of possible bioactive secondary metabolites is sparked by the discovery of many polyketide synthase and non-ribosomal peptide synthetase genes of unknown function from sequenced Rhodococcus genomes. Rhodococcus species strain MTM3W5.2 was recently shown to produce a strong inhibitory compound with activity against most strains of Rhodococcus and closely related genera. A goal of this investigation is to discover the gene(s) required to synthesize this inhibitory molecule. The engineered Rhodococcus …

Minority College Students’ Attitudes And Beliefs Regarding The Profession Of Dental Hygiene In Comparison To Their Oral Health And Dental Knowledge, Trina J. Morgan

Electronic Theses and Dissertations

The purpose of this study was to find out the attitudes and beliefs of minority college students enrolled at Missouri College in Brentwood, Missouri in reference to the dental hygiene profession. In particular, does their oral health and dental knowledge relate to their knowledge of profession of dental hygiene? One hundred and six students gave their consent to participate in the study via Survey Monkey. The study was conducted in May 2015 for a period of four weeks. Four statements were designed to gauge minority students’ knowledge of dental hygiene as a career. No differences were found based on gender, …

Leishmaniasis, Malaria, And Schistosomiasis Concurrently In An 8-Year-Old Boy, Mahmood D. Al-Mendalawi

Saudi Medical Journal

No abstract provided.

Enhancement Of Hiv Persistence By Adipose Tissue, Jacob Couturier

Dissertations and Theses (Open Access)

A major challenge for HIV eradication is the persistence of cellular and anatomic viral reservoirs. The primary cellular reservoirs for HIV, memory CD4 T cells and macrophages, traffic into various tissues where most viral replication occurs, or in the case of virally-suppressed antiretroviral-treated patients, the virus becomes dormant and protected from drugs and immune responses. The major tissue reservoirs for HIV include lymphoid tissues such as lymph nodes, bone marrow, and gastrointestinal lymphoid tissues, but adipose tissues could potentially harbor HIV as well. Adipose tissues are ubiquitous connective tissues and major sites of infiltration and immune activity by T cells …

Computational Modeling Of Rna-Small Molecule And Rna-Protein Interactions, Lu Chen

Dissertations and Theses (Open Access)

The past decade has witnessed an era of RNA biology; despite the considerable discoveries nowadays, challenges still remain when one aims to screen RNA-interacting small molecule or RNA-interacting protein. These challenges imply an immediate need for cost-efficient while predictive computational tools capable of generating insightful hypotheses to discover novel RNA-interacting small molecule or RNA-interacting protein. Thus, we implemented novel computational models in this dissertation to predict RNA-ligand interactions (Chapter 1) and RNA-protein interactions (Chapter 2).

Targeting RNA has not garnered comparable interest as protein, and is restricted by lack of computational tools for structure-based drug design. To test the potential …

Regulation Of Autoimmune Germinal Center Reaction By Tfh Cells And Application Of Tfr-Like Cells For The Treatment Of Autoimmune B Cell Responses, Young Uk Kim

Dissertations and Theses (Open Access)

Excessive follicular helper T (Tfh) cell responses to self-antigens are associated with antibody-mediated autoimmune diseases in humans including systemic lupus erythematosus (SLE). Numeral and functional aberrations of T regulatory T (Treg) cells are common in patients with autoimmune diseases. Although different types of immunosuppressive agents have been used clinically to treat antibody-mediated autoimmune diseases, they generally have side effects due to the lack of target-specificity. To minimalize the adverse effects, there is a need to develop target-specific therapeutics which specifically control auto-reactive B cell responses and auto-reactive Tfh cell responses. Recent studies unveiled that Foxp3⁺ Treg cells expressing CXCR5 …

Virulence Evolution Of Fungal Pathogens In Social And Solitary Bees With An Emphasis On Multiple Infections, Ellen G. Klinger

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The health of pollinators, especially bees, is of the utmost importance to success of many agricultural ecosystems. Microorganisms can cause diseases in bees; such microbes are pathogenic. The ability of a pathogen to cause harm to its host (such as a bee) is termed its virulence. Studying the evolution of different levels of virulence can lead researchers to a better understanding of pathogens, and potentially predict how much harm a pathogen can cause in the future. We studied the evolution of virulence levels for a fungal disease of bees. This group of fungi is composed of 28 species, and some …

Evaluation Of Vaccination Policies Among Outpatient Oncology Clinics In Utah: A Pilot Study, Sarah Louise Stocksdale

Theses and Dissertations

Background: In Utah, all major hospital facilities have employee vaccination policies. However, the presence of health care worker vaccination policies in the Utah outpatient oncology setting was unknown. Objectives: The objectives were to identify Utah oncology outpatient employee vaccination policies and to identify what consequences, if any, were present for unvaccinated employees. Methods: This was a cross-sectional, descriptive study design in which clinic managers from outpatient oncology clinics in Utah were asked, via questionnaire, to describe the clinic's employee vaccination policy and the consequences for refusing the policy. Findings: Most vaccination policies applied to employees primarily assigned to work in …

Evidence For The Persistence Of Food Web Structure After Amphibian Extirpation In A Neotropical Stream, Thomas R. Barnum, John M. Drake, Jose Checo Colón-Gaud, Amanda T. Rugenski, Therese C. Frauendorf, Scott Connelly, Susan S. Kilham, Matt R. Whiles, Karen R. Lips, Catherine M. Pringle

Biology: Faculty Publications

Species losses are predicted to simplify food web structure, and disease‐driven amphibian declines in Central America offer an opportunity to test this prediction. Assessment of insect community composition, combined with gut content analyses, was used to generate periphyton–insect food webs for a Panamanian stream, both pre‐ and post‐amphibian decline. We then used network analysis to assess the effects of amphibian declines on food web structure. Although 48% of consumer taxa, including many insect taxa, were lost between pre‐ and post‐amphibian decline sampling dates, connectance declined by less than 3%. We then quantified the resilience of food web structure by calculating …

The Role Of The State In Singapore: Pragmatism In Pursuit Of Growth, Kim Song Tan, Manu Bhaskaran

Research Collection School Of Economics

This paper looks at how government intervention shapes the evolution of the Singapore economy and accounts for its successes and failures over the past 50 years. Compared with other dynamic Asian economies, the Singapore government's approach to intervene in the economy is both more extensive and more intrusive, but with a narrow focus on GDP growth and surplus accumulation as the primary objectives. The ruling government's near complete dominance in politics has enabled it to mobilize resources to create the preconditions for strong GDP growth and high savings. But the impact on the broader development of the economy and the …

Autologous Hematopoietic Stem Cell Transplantation-10 Years Of Data From A Developing Country, Natasha Ali, Salman Naseem Adil, Mohammad Usman Shaikh

Department of Pathology and Laboratory Medicine

Intensive chemotherapy followed by autologous stem cell transplantation is the treatment of choice for patients with hematological malignancies. The objective of the present study was to evaluate the outcomes of patients with mainly lymphoma and multiple myeloma after autologous stem cell transplant. The pretransplant workup consisted of the complete blood count, an evaluation of the liver, kidney, lung, and infectious profile, chest radiographs, and a dental review. For lymphoma, all patients who achieved at least a 25% reduction in the disease after salvage therapy were included in the study. Mobilization was done with cyclophosphamide, followed by granulocyte colony-stimulating factor, 300 …

Status Of Policies On Hiv/Aids: Responses Of Nebraska Postsecondary Institutions With Health Occupations Programs, Jean Durgin-Clinchard M.A., Dorothy M. Witmer Ed.D., R.N., James Brown R.N., J.D.

Journal of Health Occupations Education

This study was conducted to assess the status of HIV/AIDS policies in postsecondary health occupations programs in Nebraska. The purposes of the study were to determine which postsecondary health occupations education programs had policies adequate to address HIV/AIDS, and to review a copy of the program’s policies for agreement with a set of criteria developed from those recommended by the Centers for Disease Control and the American College Health Association. Results revealed that the institutions’ policies submitted for review did not meet the criteria developed for the study.

A Study Of Aids Policies As Reported By Practical Nursing Programs Of Indiana, David K. Miller Rnc, B.S.N., M.S. Ed, Karen E. Gable Ed.D.

Journal of Health Occupations Education

Many of the nation’s nursing schools have yet to develop AIDS policies. Without such policy implementation, nursing educators face many problems. A recent study of nursing programs in Nebraska revealed that none of the schools’ policies met all of the twenty-one criteria set forth by the Centers for Disease Control (CDC) and the American College Health Association (ACHA). The purposes of this study were to determine how many of Indiana’s practical nursing programs have created or implemented AIDS policies and to compare and contrast those policies with the criteria set by Witmer (1992), the CDC, and the ACHA. A self-reporting …

Development Of An Integrated Human Science Course For Science Credit In The Indianapolis Public Schools, Rosie L. Hicks

Journal of Health Occupations Education

For the first time, in the state of Indiana, a model and precedent for meeting minimum high school science graduation requirements with a health occupations program has been accomplished. Because of the national and state thrust on improving education by increasing requirements for graduation and for sciences in particular, this model was developed to facilitate student completion of science courses prior to in-depth health occupations coursework. The Indianapolis Public Schools were granted permission to offer a four semester, eight-credit Integrated Human Studies course at the Health Professions Center during the summer of 1987. The course provides four credits in the …

Establishing A Protocol For The Long-Term Sampling And Analysis Of Tick-Borne Pathogens In The Lehigh Valley, Rachel Heist

Research Scholars Poster Presentation

Establishing a Protocol for the Long-term Sampling and Analysis of Tick-borne Pathogens in the Lehigh Valley

Rachel Heist¹

¹Department of Infectious Diseases, Lehigh Valley Health Network

Abstract

The blackedlegged tick, Ixodes scapularis, transmits several human pathogens including the spirochete that causes Lyme disease (Borellia burgdorferi), which is endemic to the Lehigh Valley region of eastern Pennsylvania. Additional tick-borne diseases have also been emerging in the region including tick-borne relapsing fever (caused by Borrelia miyamotoi), human granulocytic anaplasmosis (caused by Anaplasma phagocytophilum), and human babesiosis (caused by Babesia microti). The dog tick, Dermacentor variabilis, …

Hospital-Wide Impact Of Mandatory Infectious Disease Consultation On Staphylococcus Aureus Septicemia, Amanda Guth

Research Scholars Poster Presentation

Hospital-wide Impact of Mandatory Infectious Disease Consultation on Staphylococcus aureus Septicemia

Amanda Guth¹

Amy Slenker MD^1,2

¹Department of Infectious Diseases, Lehigh Valley Health Network

²Research Scholar Program Mentor

Abstract

Staphylococcus aureus bacteremia (SAB) is associated with considerable morbidity mortality¹. Mandatory Infectious Diseases (ID) consultation for SAB was introduced to the Lehigh Valley Health Network in order to improve the care of SAB patients at both the Cedar Crest and Muhlenberg campuses. This study sought to determine if mandatory ID consultation improves adherence to published guidelines on quality-of-care indicators and improves outcomes of patients with …

Agave Chewing And Dental Wear: Evidence From Quids, Emily E. Hammerl, Melissa A. Baier, Karl Reinhard

Karl Reinhard Publications

Agave quid chewing is examined as a potential contributing behavior to hunter-gatherer dental wear. It has previously been hypothesized that the contribution of Agave quid chewing to dental wear would be observed in communities wherever phytolith-rich desert succulents were part of subsistence. Previous analysis of coprolites from a prehistoric agricultural site, La Cueva de los Muertos Chiquitos in Durango, Mexico, showed that Agave was a consistent part of a diverse diet. Therefore, quids recovered at this site ought to be useful materials to test the hypothesis that dental wear was related to desert succulent consumption. The quids recovered from the …

Atrial Fibrillation In Sub-Saharan Africa: Epidemiology, Unmet Needs, And Treatment Options, Bruce Sheldon Stambler, Mzee Ngunga

Internal Medicine, East Africa

Health care in Sub-Saharan Africa is being challenged by a double burden of disease as lifestyle diseases common in the developed world, such as stroke and atrial fibrillation (AF), increase, while, simultaneously, health issues of the developing world in terms of communicable disease persist. The prevalence of AF is lower in Africa than in the developed world but is expected to increase significantly over the next few decades. Patients with AF in Africa tend to be younger and have a higher prevalence of rheumatic valvular heart disease than patients with AF in other regions. Permanent AF is the most prevalent …

Unraveling Host-Vector-Arbovirus Interactions By Two-Gene High Resolution Melting Mosquito Bloodmeal Analysis In A Kenyan Wildlife-Livestock Interface, David Omondi, Daniel K. Masiga, Yvonne Ukamaka Ajamma, Burtram C. Fielding, Laban Njoroge, Jandouwe Villinger

All Peer-Reviewed Publications

The blood-feeding patterns of mosquitoes are directly linked to the spread of pathogens that they transmit. Efficient identification of arthropod vector bloodmeal hosts can identify the diversity of vertebrate species potentially involved in disease transmission cycles. While molecular bloodmeal analyses rely on sequencing of cytochrome b (cyt b) or cytochrome oxidase 1 gene PCR products, recently developed bloodmeal host identification based on high resolution melting (HRM) analyses of cyt b PCR products is more cost-effective. To resolve the diverse vertebrate hosts that mosquitoes may potentially feed on in sub-Saharan Africa, we utilized HRM profiles of both cyt b and 16S …

Evaluation Of Cardiac Involvement In Children With Dengue By Serial Echocardiographic Studies, Tawatchai Kirawittaya, In-Kyu Yoon, Sineewanlaya Wichit, Sharone Green, Francis A. Ennis, Robert V. Gibbons, Stephen J. Thomas, Alan L. Rothman, Siripen Kalayanarooj, Anon Srikiatkhachorn

Institute for Immunology and Informatics Faculty Publications

Background:

Infection with dengue virus results in a wide range of clinical manifestations from dengue fever (DF), a self-limited febrile illness, to dengue hemorrhagic fever (DHF) which is characterized by plasma leakage and bleeding tendency. Although cardiac involvement has been reported in dengue, the incidence and the extent of cardiac involvement are not well defined.

Methods and Principal Findings:

We characterized the incidence and changes in cardiac function in a prospective in-patient cohort of suspected dengue cases by serial echocardiography. Plasma leakage was detected by serial chest and abdominal ultrasonography. Daily cardiac troponin-T levels were measured. One hundred and eighty …

Competing Infant Feeding Information In Mothers’ Networks: Advice That Supports V. Undermines Clinical Recommendations, Sato Ashida, Freda B. Lynn, Natalie A. Williams

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: To identify the social contextual factors, specifically the presence of information that supports v. undermines clinical recommendations, associated with infant feeding behaviours among mothers in low-income areas.

Design: Cross-sectional survey evaluating social support networks and social relationships involved in providing care to the infant along with feeding beliefs and practices.

Setting: Out-patient paediatric and government-funded (Women, Infants, and Children) clinics in an urban, low-income area of the south-eastern USA.

Subjects: Eighty-one low-income mothers of infants between 0 and 12 months old.

Results: Most mothers reported receiving both supportive and undermining advice. The presence of …

Competing Infant Feeding Information In Mothers’ Networks: Advice That Supports V. Undermines Clinical Recommendations, Sato Ashida, Freda B. Lynn, Natalie A. Williams, Ellen J. Schafer

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: To identify the social contextual factors, specifically the presence of information that supports v. undermines clinical recommendations, associated with infant feeding behaviours among mothers in low-income areas.

Design: Cross-sectional survey evaluating social support networks and social relationships involved in providing care to the infant along with feeding beliefs and practices.

Setting: Out-patient paediatric and government-funded (Women, Infants, and Children) clinics in an urban, low-income area of the south-eastern USA.

Subjects: Eighty-one low-income mothers of infants between 0 and 12 months old.

Results: Most mothers reported receiving both supportive and undermining advice. The presence …

Effect Of Compatibilisers On Mechanical, Barrier And Antimicrobial Properties Of Ipp/Zno Nano/Microcomposites For Food Packaging Application, Sossio Cimmino, Donatella Duraccio, Antonella Marra, Marilena Pezzuto, Ida Romano, Clara Silvestre

Journal of Applied Packaging Research

It is investigated the effects of the addition of three PPgMA, with different molecular weight and maleic anhydride content, on the structure, morphology, mechanical, thermal and antimicrobial properties and oxygen permeability of iPP/ZnO 98/2 wt% composite.

The composition of iPP/PPgMA/ZnO composites is fixed at 88/10/2 wt%, that is, about 10 wt% of iPP is substituted with PPgMA. The composites are prepared via melt mixing by using a twin-screw extruder. The ZnO is obtained in a preindustrial scale spray pyrolysis platform. The Fourier Transform Infrared spectroscopy indicates that the ZnO particles, in all the ternary composites, react with maleic anhydride groups …

Geospatial Resolution Of Human And Bacterial Diversity With City-Scale Metagenomics, Ebrahim Afshinnekoo, Cem Meydan, Shanin Chowdhury, Dyala Jaroudi, Collin Boyer, Nick Bernstein, Julia M. Maritz, Darryl Reeves, Jorge Gandara, Sagar Chhangawala, Sofia Ahsanuddin, Amber Simmons, Timothy Nessel, Bharathi Sundaresh, Elizabeth Pereira, Ellen Jorgensen, Sergios-Orestis Kolokotronis, Nell Kirchberger, Isaac Garcia, David Gandara, Sean Dhanraj, Tanzina Nawrin, Yogesh Saletore, Noah Alexander, Priyanka Vijay, Elizabeth M. Hénaff, Paul Zumbo, Michael Walsh, Gregory D. O'Mullan, Scott Tighe, Joel T. Dudley, Anya Dunaif, Sean Ennis, Eoghan O'Halloran, Tiago R. Magalhaes, Braden Boone, Angela L. Jones, Theodore R. Muth, Katie Schneider Paolantonio, Elizabeth Alter, Eric E. Schadt, Jeanne Garbarino, Robert J. Prill, Jane M. Carlton, Shawn Levy, Christopher E. Mason

Publications and Research

The panoply of microorganisms and other species present in our environment influence human health and disease, especially in cities, but have not been profiled with metagenomics at a city-wide scale. We sequenced DNA from surfaces across the entire New York City (NYC) subway system, the Gowanus Canal, and public parks. Nearly half of the DNA (48%) does not match any known organism; identified organisms spanned 1,688 bacterial, viral, archaeal, and eukaryotic taxa, which were enriched for harmless genera associated with skin (e.g., Acinetobacter). Predicted ancestry of human DNA left on subway surfaces can recapitulate U.S. Census demographic data, and bacterial …

Repurposing Celecoxib As A Topical Antimicrobial Agent, Shankar Thangamani, Waleed Younis, Mohamed N. Seleem

Department of Comparative Pathobiology Faculty Publications

There is an urgent need for new antibiotics and alternative strategies to combat multidrug-resistant bacterial pathogens, which are a growing clinical issue. Repurposing existing approved drugs with known pharmacology and toxicology is an alternative strategy to accelerate antimicrobial research and development. In this study, we show that celecoxib, a marketed inhibitor of cyclooxygenase-2, exhibits broad-spectrum antimicrobial activity against Gram-positive pathogens from a variety of genera, including Staphylococcus, Streptococcus, Listeria, Bacillus, and Mycobacterium, but not against Gram-negative pathogens. However, celecoxib is active against all of the Gram-negative bacteria tested, including strains of, Acinetobacter, and Pseudomonas, when …

Bjc Today

BJC Today

No abstract provided.

Tight Junction Cldn2 Gene Is A Direct Target Of The Vitamin D Receptor, Yong-Guo Zhang, Shaoping Wu, Rong Lu, David Zhou, Jingsong Zhou, Geert Carmeliet, Elaine Petrof, Erika C. Claud, Jun Sun

Faculty and Staff Publications

The breakdown of the intestinal barrier is a common manifestation of many diseases. Recent evidence suggests that vitamin D and its receptor VDR may regulate intestinal barrier function. Claudin-2 is a tight junction protein that mediates paracellular water transport in intestinal epithelia, rendering them "leaky". Using whole body VDR(-/-) mice, intestinal epithelial VDR conditional knockout (VDR(ΔIEC)) mice, and cultured human intestinal epithelial cells, we demonstrate here that the CLDN2 gene is a direct target of the transcription factor VDR. The Caudal-Related Homeobox (Cdx) protein family is a group of the transcription factor proteins which bind to DNA to regulate the …

Addressing Therapeutic Options For Ebola Virus Infection In Current And Future Outbreaks, Azizul Haque, Didier Hober, Joel Blondiaux

Dartmouth Scholarship

Ebola virus can cause severe hemorrhagic disease with high fatality rates. Currently, no specific therapeutic agent or vaccine has been approved for treatment and prevention of Ebola virus infection of humans. Although the number of Ebola cases has fallen in the last few weeks, multiple outbreaks of Ebola virus infection and the likelihood of future exposure highlight the need for development and rapid evaluation of pre- and postexposure treatments. Here, we briefly review the existing and future options for anti-Ebola therapy, based on the data coming from rare clinical reports, studies on animals, and results from in vitro models. We …