Digital Commons Network^™

Kinesiology 183 Advanced First Aid, Cpr, And Aed Spring 2015, Jessica Mcmahon

Kinesiology Courses

No abstract provided.

The Role Of Batf2 In Lps/Ifnγ Polarized Macrophages, Marie A. Gehman

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

Transcription factors regulate distinct macrophage functions by regulating gene expression in response to micro-environmental cues. This functional plasticity is critical for regulating innate and adaptive immune responses during infection and during chronic disease processes including inflammatory diseases and cancer. Microarray analysis of macrophages polarized to a pro-inflammatory (M1) phenotype with LPS and IFNγ revealed that basic leucine zipper transcription factor ATF-like 2 (Batf2), a member of the AP1 transcription factors, is selectively upregulated in M1 macrophages compared to anti-inflammatory IL-4-polarized (M2) macrophages. The initial hypothesis was that Batf2 is a master regulator of gene expression that orchestrates M1 polarization. To …

Antibiotics Targeting Tuberculosis: Biosynthesis Of A-102395 And Discovery Of Novel Actinomycins, Wenlong Cai

Theses and Dissertations--Pharmacy

The increase in antibiotic resistance of many bacterial strains including multidrug-resistant tuberculosis (MDR-TB) due to over- and misuse of antibiotics is a serious medical and economical problem. Therefore discovery and development of new antibiotics are urgently needed. Two projects were undertaken to address the need for new anti-tuberculosis antibiotics.

1. Discovery of new chemical entities. A-102395, a new nucleoside inhibitor of bacterial MraY (translocase I, EC 2.7.8.13) that is essential for bacterial survival, was isolated from the culture broth of Amycolatopsis sp. SANK 60206 in 2007. Although A-102395 is a potent inhibitor of translocase I with IC50 of 11 nM, …

Elucidating The Mechanism Of Lipl: A Non-Heme Fe(Ii), Α -Ketoglutarate: Uridine-5’-Monophosphate Dioxygenase, Anwesha Goswami

Theses and Dissertations--Pharmacy

Several nucleoside natural product antibiotics from Streptomyces sp. and actinomycetes have recently been shown to target bacterial peptidoglycan cell wall biosynthesis by inhibiting the bacterial translocase I (MraY). The biosynthetic gene clusters for A-90289, liposidomycins and caprazamycins revealed a protein with sequence similarity to proteins annotated as α-KG:taurine dioxygenases (TauD). This enzyme (LipL) is a mononuclear, non-heme, Fe(II) dependent α-keto glutarate (α-KG) :uridine monophosphate (UMP) dioxygenase responsible for the net dephosphorylation and two electron oxidation of UMP to uridine-5’-aldehyde. The postulated reaction coordinates involving the activation of the C-5’ center in UMP and the corresponding formation of uridine-5’-aldehyde are modeled …

Nonlinear Hierarchical Models For Longitudinal Experimental Infection Studies, Michael David Singleton

Theses and Dissertations--Epidemiology and Biostatistics

Experimental infection (EI) studies, involving the intentional inoculation of animal or human subjects with an infectious agent under controlled conditions, have a long history in infectious disease research. Longitudinal infection response data often arise in EI studies designed to demonstrate vaccine efficacy, explore disease etiology, pathogenesis and transmission, or understand the host immune response to infection. Viral loads, antibody titers, symptom scores and body temperature are a few of the outcome variables commonly studied. Longitudinal EI data are inherently nonlinear, often with single-peaked response trajectories with a common pre- and post-infection baseline. Such data are frequently analyzed with statistical methods …

Emotional Enhancement And Repetition Effects During Working Memory In Persons With Mild Cognitive Impairment, Lucas S. Broster

Theses and Dissertations--Clinical and Translational Science

This dissertation introduces a framework for understanding differences in how emotional enhancement effects might influence memory in aging adults and then summarizes the findings of three studies of how repetition effects and emotional enhancement effects influence working memory in older adults without cognitive impairment (NC), older adults with amnestic mild cognitive impairment (MCI), and older adults with mild Alzheimer’s disease (AD). In these experiments, individuals with AD showed cognitive impairment in terms of accuracy and reaction time, but individuals with MCI showed milder behavioral impairment that was confined to manipulations of working memory. Individuals with AD showed relative sparing of …

An Investigation Into The Occurrence Of Batrachochytrium Dendrobatidis Infection In Plethodontid Salamander Communities Of Robinson Forest, Sarah H. Spaulding

Theses and Dissertations--Forestry and Natural Resources

Environmental and anthropogenic stressors negatively affect amphibians in a variety of ways, often increasing their vulnerability to pathogen infection and mortality. Sampling for the pathogenic fungus Batrachochytrium dendrobatidis (Bd) was conducted in order to: 1) determine the presence of chytrid infection in stream-associated plethodontid salamanders of southeastern Kentucky, and 2) evaluate differences in infection intensity between salamanders residing in intact forest streams, timber-harvested streams, and surface-mined streams. During 14 sampling sessions occurring between March, April and May of 2013, DNA samples from 306 individual salamanders within 8 species from the family Plethodontidae were collected; additional amphibians (i.e. frogs, newts) were …

Reversible Downregulation Of Hypothalamic-Pituitary-Gonadal Axis In The Stallion With A Third-Generation Gnrh Antagonist, Gabriel Monteiro Davolli

Theses and Dissertations--Veterinary Science

The objectives of this thesis were: (1) to evaluate the downregulation of the stallion hypothalamic-pituitary-gonadal (HPG) axis by a GnRH antagonist (acyline) based upon endocrine, seminal, testicular and behavioral effects, and (2) to assess recovery after treatment. Stallions were treated for 50 days (n=4; 330µg/kg acyline q 5d) and controls (n=4) received vehicle alone. Stallions were assessed pre-treatment and for 72 days after last treatment. Treatment induced declines (p<0.05) in FSH, LH, testosterone (to castrate levels) and estrone sulfate. Gonadotropins and testosterone returned to control values within nine days and estrone sulfate by 14 days after treatment discontinuation. Acyline-treated stallions failed to respond with FSH, LH and testosterone increase after exogenous GnRH stimulation (25µg gonadorelin, IV) compared to pre-treatment and control stimulation. Total sperm numbers and motility were reduced in acyline-treated stallions, as well as total seminal plasma protein and testicular volume (p<0.05). Time to ejaculation was increased in acyline group (p<0.5). Testicular, sexual behavior and most seminal parameters regained normal levels within 72 days after treatment ceased. Sperm output of acyline-treated stallions was regained within seven months after ending treatment. Acyline reversibly suppressed the stallion HPG axis, thus has potential for treating the androgen-dependent Equine-Arteritis-Virus carrier state and as behavior modulator.

Food Security Factors Affect Growth In Young Children In An Ecuadorian Indigenous Community, Whitney Jeannine Asher

Theses and Dissertations--Nutrition and Food Systems

Inadequate nutrition in preschool-aged children in an urban indigenous community outside of Santo Domingo, Ecuador has a negative impact on growth and development. Nutritional assessments have shown that children are underweight and that there are some effects of stunting and wasting in the population. This study was conducted to assess the extent of food security in this indigenous community in preparation for addressing two of the Millennium Development Goals for 2015: reducing the under-five child mortality rate by two-thirds and eradicating extreme poverty and hunger. An aim was to validate on-site assessment measures in this indigenous community regarding geographic circumstances, …

Mucosal Delivery Of Particulate Breast Cancer Vaccine, Lipika Chablani, Nihal S. Mulla, Periasamy Selvaraj, Martin J. D'Souza

Pharmacy Faculty/Staff Publications

Vaccination has been widely used as a mode of protection against various diseases by taking advantage of host's immune system. Even though vaccination has provided relief from many infectious diseases, vaccination for cancer still remains a challenge. Cancer is caused by mutated cell functioning leading to uncontrolled growth in the organ of genesis and further possible metastasis worsens the situation. In spite of various current therapies such as surgery, chemotherapy and radiation therapy, we are still lacking behind in the race with this evolving disease. There are two major approaches for vaccination: prophylactic or therapeutic. Prophylactic vaccines find their applications …

N.Y.C. Soda Ban Misconceptions: Why The Real Issue Is Not Fundamental, Christine Mansour

Student Works

No abstract provided.

The Struggle Of Regulating Clinical Trials In A Developing Nation: India’S Experience, Arianna Moure

Student Works

No abstract provided.

Essay: Pharmaceuticals And Global Justice: Balancing Public Health And Intellectual Property Rights, Marisa Morabito

Student Works

No abstract provided.

Functional Connection Between Yeast Dynamin And Retromer At The Endosome, Christopher Robert Trousdale

Graduate Theses/Dissertations

Intracellular trafficking from the late endosome to Golgi in cells is termed retrograde transport, essential for recycling of important macromolecules including cell membrane receptors. Retrograde transport is regulated by a family of proteins known as the "Retromer” composed of 5 VPS proteins (Vps5, Vps17, Vps26, Vps29, and Vps35). Retromer acts as the coat proteins for vesicles emerging from late endosomes. Loss of Retromer function has been previously implicated in both Parkinson's and Alzheimer's disease. Vps1, a yeast dynamin-like protein, plays a role in intracellular trafficking. Vps1 has been shown to localize at the endocytic sites to promote pinching off of …

Reintroduction Biology Of Head-Started Ornate Box Turtles, Eric R. Sievers

Graduate Theses/Dissertations

The ornate box turtle (Terrapene ornata) is a prairie-dwelling species that has experienced population declines, especially near the northern edge of its range. In order to provide supporting research for a reintroduction program at the Upper Mississippi River National Wildlife and Fish Refuge in northwestern Illinois, I compared the relative success of different approaches to reintroduction. Specifically, I tested the null hypothesis that reintroduced turtles exhibited equal reintroduction success when, 1) released at a site that is known to support a viable ornate box turtle population, 2) ‘soft-released' in a fenced enclosure at a site where very few ornate box …

Privatization And Its Discontents: The Troubling Record Of Privatized Prison Health Care, Dan Weiss

University of Colorado Law Review

As part of a decades-long national trend towards privatization, local governments have turned to private contractors to provide health care in American prisons and jails. Ostensibly, the driving force behind this phenomenon is a desire to cut costs in an era of expanding prison health care expenditures and contracting governmental revenue streams. Local government officials build the cost-cutting incentive directly into their contracts via fixed-rate payment structures and cost-sharing provisions for off-site emergency treatment. While these contracts encourage cost-reduction, they simultaneously discourage proper oversight; their indemnification clauses render local governments largely immune from financial consequences when contractors deny emergency care …

'Makin' It Out': The Cost Of Dropping Out Of High School On The Health Status Of Afro-American Women In Urban Slums, Sesa E. Bakenra-Tikande

CMC Senior Theses

“We carry our histories in our bodies, how could we not?” – Nancy Krieger

In the United States and abroad, socioeconomic status (income, education, and occupation) greatly impacts health outcomes for a given population. There is a strong and consistent socioeconomic gradient within health outcomes which has been documented as far back as in Ancient Egypt and China (Krieger, Willains, & Moss, 1997; Liberatos, Link, & Kelsey, 1988) The general trend shows that individuals with higher socioeconomic status generally enjoy lower rates of morbidity (disease) and disability, which can ultimately lead to higher mortality rates (House et al. (1992) and …

Relationship Between Modality And The Degree Of Knowledge Retention In Bioterrorism Training, Gaylon Rashun Crawford

Walden Dissertations and Doctoral Studies

A public health workforce must be trained to react quickly, especially in the case of terrorist attack. Political leaders and emergency management experts have often cited inadequate emergency training as a contributing factor in the public health system's failed preparations for a bioterrorist event. As a result of these failures, billions of dollars have been allocated towards correcting infrastructure deficiencies including training for public health nurses (PHNs), who are critical to a communitywide medical response. This quantitative study used Pearson's correlation and a multivariate regression analysis to evaluate the most effective modality of bioterrorism training (BTT) for PHNs working in …

Improving Nurses' Knowledge To Reduce Catheter-Related Bloodstream Infection In Hemodialysis Unit, Mohammed Jawad Kadium

Walden Dissertations and Doctoral Studies

Central venous catheters (CVCs) are commonly used as vascular access for patients who require hemodialysis. Infectious complications are a serious clinical problem, and they are associated with high rates of morbidity and mortality, prolonged hospital stay, and increased medical treatment costs. The purpose of theproject was to evaluate the effectiveness of educating registered dialysis nurses regarding CVC maintenance care to reduce catheter-related bloodstream infection (CRBSI) in a hemodialysis unit. The project question focused on the educational program derived from the evidence-based guidelines recommended by the Centers for Disease Control and Prevention (CDC) to improve registered dialysis nurses' knowledge regarding CVC …

Rabies: The Interplay Of One Virus With Medicine Throughout History, Anita Maria Roberti

Theses & ETDs

Rabies is a deadly neuropathic virus, which is transmitted through the saliva of an infected animal. It is one of the first diseases on record in ancient medical references. It was so recognizable and lethal that it had a reputation of mythic proportions throughout most of human history. In 1885, Louis Pasteur tested the first rabies vaccine on a human. The vaccine worked, and ushered in a new era of medical research. Over the years, the treatments to prevent rabies have been improved, increasing their effectiveness. Recent medical research is even attempting to make use of the virus’s unique properties …

African American Eighth Grade Students' Attitudes Toward Hiv/Aids In The District Of Columbia, Enock Kolawole Adewuyi

Walden Dissertations and Doctoral Studies

The group most affected by HIV/AIDS, according to the Centers for Disease Control and Prevention (CDC), is African Americans. The purpose of this study was to explore the knowledge of HIV/AIDS held by African American children as a first step towards developing prevention strategies for these youths. In order to bridge the knowledge-behavior gap, this study sought to investigate the attitude towards HIV/AIDS of African American 8th grade students. The study involved secondary data from the 2012 District of Columbia (DC) Middle School Youth Risk Behavior Survey, obtained from the District of Columbia Office of Superintendent of Education (OSSE). Guided …

Barriers To Receiving The Influenza Vaccine In Adults 65 Years And Older, Melissa Madalone

Walden Dissertations and Doctoral Studies

Vaccination is regarded by many as the most effective means of reducing influenza infection and disease; however, many people in the United States are hospitalized from flu-related illness each year. Adults 65 years and older account for more than half of these hospitalizations and almost all flu-related deaths. This project aimed to identify barriers to receiving the influenza vaccine among the adult population (> 65 years of age) in a community setting. The goal was to develop a teaching tool that would assist practitioners towards improving influenza vaccination rates among this population. The Health Belief Model was the theoretical framework …

Physiological Changes In White-Rot Fungi In Response To Simulated Nitrogen Deposition Are Not Readily Reversed Through Experimental Evolution, Elizabeth Amy Landis

Master's Theses and Capstones

Increases in nitrogen (N) deposition have been shown to slow decomposition of organic material including lignin, particularly in forests with high lignin content. Since basidiomycete fungi are the primary decomposers of lignin, their response to increases in available N are of interest. The Chronic Nitrogen Amendment Study at the Harvard Forest Long-Term Ecological Research (CNAS HF-LTER) site is an experimental N gradient in Petersham, MA. Since 1988, three megaplots have received varying levels of NH4NO3: ambient-only deposition (control), 50 kg N ha-1 yr-1 (N50), or 150 kg N ha-1 yr-1 (N150). In order to examine how species of lignin-degrading basidiomycetes …

Novel Delivery Systems For Iron Replenishment, Naresh Modepalli

Electronic Theses and Dissertations

Iron is an integral part of hemoglobin and essential for the production of red blood cells. Iron deficiency and the resulting anemia are major nutritional deficiency disorders. The majority of patient populations suffering from iron deficiency anemia (IDA) are women of child bearing age and children of all ages. Iron deficiency is a complication of various other chronic conditions. Oral iron salts or colloidal parenteral iron formulations are treatment options for iron replenishment since several decades, but they are associated with severe side effects along with other patient noncompliance issues. Transdermal delivery of iron could be a potential alternative to …

Medicine@Yale October/November 2015 Volume 11, Issue 2, Yale School Of Medicine Office Of Communications

Medicine@Yale - development newsletter

Medicine@Yale : advancing biomedical science, education and health care was a print and online newsletter which published news about academics, research and philanthropy for alumni, donors, faculty, students and the general public. It was initially published 6 times a year and then changed to a quarterly schedule. The first issue came out June/Jul. 2005. The final issue was published in May/June 2022, vol. 17, issue 1. Published by Yale School of Medicine, Office of Communications.

Opportunities To Improve Postpartum Care For Mothers And Infants: Design Of Context-Specific Packages Of Postpartum Interventions In Rural Districts In Four Sub-Saharan African Countries, Els Duysburgh, Birgit Kerstens, Seni Kouanda, Charles Paulin Kaboré, Danielle Belemsaga Yugbare, Peter Gichangi, Gibson Masache, Beatrice Crahay, Gilda Gondola Sitefane, Nafissa Bique Osman, Severiano Foia, Henrique Barros, Sofia Castro Lopes, Susan Mann, Bejoy Nambiar, Tim Colbourn, Marleen Temmerman

Obstetrics and Gynaecology, East Africa

Background: Postpartum maternal and infant mortality is high in sub-Saharan Africa and improving postpartum care as a strategy to enhance maternal and infant health has been neglected. We describe the design and selection of suitable, context-specific interventions that have the potential to improve postpartum care.

Methods: The study is implemented in rural districts in Burkina Faso, Kenya, Malawi and Mozambique. We used the four steps ‘systems thinking’ approach to design and select interventions: 1) we conducted a stakeholder analysis to identify and convene stakeholders; 2) we organised stakeholders causal analysis workshops in which the local postpartum situation and challenges and …

Mcgavran’S Early Years, Gary L. Mcintosh

Great Commission Research Journal

Donald McGavran was born in 1897. This article covers the years from his birth until 1923 when he and Mary McGavran sailed to India. His early life as the child of missionaries, his secondary education in the United States, service in WWI, college education at Butler University, seminary days at Yale Divinity School, and masters work at the College of Mission are presented.

Controlling Pseudomonas Aeruginosa Persister Cells By Human Granulocyte Macrophage Colony-Stimulating Factor, Geetika Sanjay Choudhary

Dissertations - ALL

Bacteria are well known to cause chronic infections in humans by entering dormancy and by developing biofilms. These mechanisms allow bacteria to exhibit antibiotic tolerance and relapse to an active virulent state when the antibiotic treatments are discontinued. During bacterial invasions, the host immune cells secrete special signaling proteins, known as cytokines which orchestrate events leading to human immune response and elimination of bacterial pathogens. However, compared to the well documented activities

of cytokines in immune reaction, little is known about the direct effects of cytokines on bacterial cells.

In this study, we focused on granulocyte macrophage colony-stimulating factor (GM-CSF), …

The Implications Of Moral Injury Among African American Females With A History Of Substance Abuse: A Preliminary Study, Jaimee Silvera Hartman

Theses and Dissertations

Moral injury is a concept that has been applied to the challenges facing veterans returning from combat due to the discrepancy between their moral values and the behaviors they engaged in due to war. In recovery women have expressed similar challenges due to the illicit behavior they engaged in while in their addiction as well as the prevalence of trauma that has impacted their transition into substance use. Thirteen female participants in treatment for substance abuse participated in this qualitative study. The majority of the women experienced a history of childhood and/or adult trauma that perpetuated their use of substances …

3 Critical Challenges For Global Health Security, Lawrence O. Gostin

Georgetown Law Faculty Publications and Other Works

International institutions are poised to make one of the most momentous decisions about the future of global health security since the formation of the World Health Organization (WHO) in 1948.

By the end of this year, 5 global commissions will have published major critiques of global health preparedness, all spurred by the Ebola epidemic, which exposed deep flaws in the international system.