Digital Commons Network^™

Leaky Intestine And Impaired Microbiome In An Amyotrophic Lateral Sclerosis Mouse Model, Shaoping Wu, Jianxun Yi, Yong-Guo Zhang, Jingsong Zhou, Jun Sun

Faculty and Staff Publications

Emerging evidence has demonstrated that intestinal homeostasis and the microbiome play essential roles in neurological diseases, such as Parkinson's disease. Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by a progressive loss of motor neurons and muscle atrophy. Currently, there is no effective treatment. Most patients die within 3-5 years due to respiratory paralysis. Although the death of motor neurons is a hallmark of ALS, other organs may also contribute to the disease progression. We examined the gut of an ALS mouse model, G93A, which expresses mutant superoxide dismutase (SOD1(G93A)), and discovered a damaged tight junction structure and …

Sexual Health Risk Behaviors Among Older Men Who Have Sex With Men: Implications For Interventions, Franco Dispenza, Brian J. Dew, Alexander K. Tatum, Edison C. M. Wolf

Adultspan Journal

No abstract provided.

Characterizing Hiv-Associated Neurocognitive Disorder In Two Underserved Sociodemographic Groups, Julia S. Seay

Open Access Dissertations

The purpose of this project is to characterize neurocognitive functioning and correlates of neurocognitive functioning, as well as to examine the diagnostic accuracy of relatively promising neurocognitive tests in two underserved sociodemographic groups living with HIV: monolingual Spanish-speaking Hispanics and English-speaking African-Americans, in order to better understand HIV-Associated Neurocognitive Disorder (HAND) within these groups. Examining HAND in underserved groups is paramount to the development of effective HAND screening and treatment algorithms in clinics that serve these groups, such as the AIDS Clinical Research Unit (ACRU) at the University of Miami. The current project was drawn from an initiative to develop …

A Reproducible Approach To High-Throughput Biological Data Acquisition And Integration, Daniela Börnigen, Yo Sup Moon, Gholamali Rahnavard, Levi Waldron, Lauren Mciver, Afrah Shafquat, Eric A. Franzosa, Larissa Miropolsky, Christopher Sweeney, Xochitl C. Morgan, Wendy S. Garrett, Curtis Huttenhower

Publications and Research

Modern biological research requires rapid, complex, and reproducible integration of multiple experimental results generated both internally and externally (e.g., from public repositories). Although large systematic meta-analyses are among the most effective approaches both for clinical biomarker discovery and for computational inference of biomolecular mechanisms, identifying, acquiring, and integrating relevant experimental results from multiple sources for a given study can be time-consuming and error-prone. To enable efficient and reproducible integration of diverse experimental results, we developed a novel approach for standardized acquisition and analysis of high-throughput and heterogeneous biological data. This allowed, first, novel biomolecular network reconstruction in human prostate cancer, …

Varying Influences Of Selection And Demography In Host-Adapted Populations Of The Tick-Transmitted Bacterium, Anaplasma Phagocytophilum Speciation And Evolutionary Genetics, Matthew Aardema, Friederike D. Von Loewenich

Department of Biology Faculty Scholarship and Creative Works

Background: The host range of a pathogenic bacterial strain likely influences its effective population size, which in turn affects the efficacy of selection. Transmission between competent hosts may occur more frequently for host generalists than for specialists. This could allow higher bacterial population densities to persist within an ecological community and increase the efficacy of selection in these populations. Conversely, specialist strains may be better adapted to their hosts and consequently achieve greater within-host population densities, with corresponding increases in selection efficacy. To assess these different hypotheses, we examined the effective population sizes of three strains of the bacterium Anaplasma …

50 Whys To Look For Genes: Pros And Complications, Peter J. Taylor

Working Papers on Science in a Changing World

“Treating the audience as capable of thinking about the complexities that surround the application of genetic knowledge” was the tagline of a series of daily blog posts made over seven weeks in the fall of 2014, posts that included extended quotes from the recently published Nature-Nurture? No (Taylor 2014). This working paper is a compilation of those posts.

A Possible Novel Therapy For A Rare But Potentially Fatal Blood Disorder, Jeff Hansen

Browse All News

No abstract provided.

Bonnie Lee Ashby, Bonnie Lee Ashby, Kelsey Duinkerken

First Women at Jefferson Oral Histories

Dr. Ashby completed her undergraduate studies at Wilson College before attending Jefferson Medical College, where she graduated in 1968. She did both her internship and residency at Bryn Mawr Hospital before accepting an infectious disease fellowship at Lankenau Hospital. Dr. Ashby is still in practice today as a general practitioner and Infectious Disease specialist in Bryn Mawr. She is also the Medical Director of the Quadrangle Life Care Community in Haverford, PA.

Daily Eastern News: March 27, 2015, Eastern Illinois University

March

No abstract provided.

Human Adipose Tissue As A Reservoir For Memory Cd4+ T Cells And Hiv, Jacob Couturier, James W. Suliburk, Jeremy M. Brown, David J. Luke, Neeti Agarwal, Xiaoying Yu, Chi Nguyen, Dinakar Iyer, Claudia A. Kozinetz, Paul A. Overbeek, Michael L. Metzker, Ashok Balasubramanyam, Dorothy E. Lewis

Faculty Publications

Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved. Objective: The objective of this study is to determine whether adipose tissue functions as a reservoir for HIV-1. Design: We examined memory CD4+ T cells and HIV DNA in adipose tissue-stromal vascular fraction (AT-SVF) of five patients [four antiretroviral therapy (ART)-treated and one untreated]. To determine whether adipocytes stimulate CD4+ T cells and regulate HIV production, primary human adipose cells were cocultured with HIV-infected CD4+ T cells. Methods: AT-SVF T cells were studied by flow cytometry, and AT-SVF HIV DNA (Gag and Env) was examined by nested PCR and sequence …

Human Adipose Tissue As A Reservoir For Memory Cd4(+) T Cells And Hiv, J Couturier, J W. Suliburk, J M. Brown, D J. Luke, J Couturier, X Y. Yu, C Nguyen, D Iyer, C A. Kozinetz, P A. Overbeek, M L. Metzker, A Balasubramanyam, D E. Lewis

Faculty Publications

Objective: The objective of this study is to determine whether adipose tissue functions as a reservoir for HIV-1. Design: We examined memory CD4(+) T cells and HIV DNA in adipose tissue-stromal vascular fraction (AT-SVF) of five patients [four antiretroviral therapy (ART)-treated and one untreated]. To determine whether adipocytes stimulate CD4(+) T cells and regulate HIV production, primary human adipose cells were cocultured with HIV-infected CD4(+) T cells. Methods: AT-SVF T cells were studied by flow cytometry, and AT-SVF HIV DNA (Gag and Env) was examined by nested PCR and sequence analyses. CD4(+) T-cell activation and HIV production were measured by …

Human Adipose Tissue As A Reservoir For Memory Cd4(+) T Cells And Hiv, J Couturier, J W. Suliburk, J M. Brown, D J. Luke, J Couturier, X Y. Yu, C Nguyen, D Iyer, C A. Kozinetz, P A. Overbeek, M L. Metzker, A Balasubramanyam, D E. Lewis

Faculty Publications

Objective: The objective of this study is to determine whether adipose tissue functions as a reservoir for HIV-1. Design: We examined memory CD4(+) T cells and HIV DNA in adipose tissue-stromal vascular fraction (AT-SVF) of five patients [four antiretroviral therapy (ART)-treated and one untreated]. To determine whether adipocytes stimulate CD4(+) T cells and regulate HIV production, primary human adipose cells were cocultured with HIV-infected CD4(+) T cells. Methods: AT-SVF T cells were studied by flow cytometry, and AT-SVF HIV DNA (Gag and Env) was examined by nested PCR and sequence analyses. CD4(+) T-cell activation and HIV production were measured by …

Immune Function And Muscle Adaptations To Resistance Exercise In Older Adults: Study Protocol For A Randomized Controlled Trial Of A Nutritional Supplement, Richard A. Dennis, Usha Ponnappan, Ralph L. Kodell, Kimberly K. Garner, Christopher M. Parkes, Melinda M. Bopp, Kalpana P. Padala, Charlotte A. Peterson, Prasad R. Padala, Dennis H. Sullivan

Physical Therapy Faculty Publications

BACKGROUND: Immune function may influence the ability of older adults to maintain or improve muscle mass, strength, and function during aging. Thus, nutritional supplementation that supports the immune system could complement resistance exercise as an intervention for age-associated muscle loss. The current study will determine the relationship between immune function and exercise training outcomes for older adults who consume a nutritional supplement or placebo during resistance training and post-training follow-up. The supplement was chosen due to evidence suggesting its ingredients [arginine (Arg), glutamine (Gln), and β-hydroxy β-methylbutyrate (HMB)] can improve immune function, promote muscle growth, and counteract muscle loss. …

Multi-Isotope Analysis To Reconstruct Dietary And Migration Patterns Of An Avar Population From Sajópetri, Hungary, Ad 568-895, Liotta Desiree Noche-Dowdy

USF Tampa Graduate Theses and Dissertations

The Avar were nomadic people from Central Asia who migrated into the Carpathian Basin in Central-Eastern Europe during the mid to late Migration Period (AD 568 - 895). Archaeological evaluation of grave goods and documentation of mortuary practices have been the primary means of understanding the Avar. However, this approach has largely neglected skeletal and biochemical analysis, in particular as these approaches relate to the biological variation, ancestry, and dietary patterns of the Avar.

There remains debate as to whether disparities existed among the socially stratified Avar population of ancient Hungary. It is argued by some that these disparities existed …

First National Kidney Graft Survival Study Of Hiv-Positive Recipients, Jeff Hansen

Browse All News

No abstract provided.

An Application Of Social Network Analysis On Military Strategy, System Networks And The Phases Of War, Thomas S. Furman

Theses and Dissertations

The research developed in this study will utilize Social Network and Graph Theory terminology and methodology applied to groups of systems, rather than individuals within a given system, in order to shape strategic level goals. With regard to military operations, Social Network Analysis has been used to show that enemy networks and relationships can be accurately represented using weighted layers with weighted relationships in order to identify the key player(s) that must be influenced and/or removed so that a particular effect on the enemy might be realized. Social Network Analysis is therefore a significant tool concerning tactical level of operations …

Molecular Modeling And Insilico Characterization Of Mutations Associated With Multiple Drug Resistant Tuberculosis (Mdr-Tb) And Extensive Drug Resistant Tuberculosis (Xdr-Tb), Sravanthi Joginipelli

Theses and Dissertations

The increasing emergence of multiple and extensive drug resistant tuberculosis poses a significant threat to effective control of tuberculosis (TB) globally. Resistance is a serious threat in the battle against the treatment and eradication of Tuberculosis. The continuing rise in tuberculosis incidence and the problem of drug resistance strains have prompted the research on developing new drug candidates and understanding the mechanism of drug resistance. Highly frequent mutations in gene sequence of mycobacterium tuberculosis were identified and were used to understand the mechanism of resistance and were computationally modeled to identify potential drug candidates for the treatment of MDR-TB and …

Awareness And Use Of E-Journals By The Scientists Of Csir-Institute Of Genomics And Integrative Biology (Igib), Delhi, And Indian Institute Of Chemical Biology (Iicb), Kolkata, India: A Comparative Study, Jamal Qasim, Abdul Mannan Khan

Library Philosophy and Practice (e-journal)

The purpose of this paper is to compare the level of awareness and use of e-journals by the scientists of Institute of Genomics and Integrative Biology (IGIB), Delhi and Indian Institute of Chemical Biology (IICB), Kolkata, India- both centre of excellence in their field of research and premier Institutes of 39 laboratories of Council of Scientific and Industrial Research (CSIR) - India's largest research and development organization. The paper is based on the results of a well structured questionnaire administered to all the scientists of the two under study institutes for the purpose of data collection supplemented by interview and …

Together, Apart. A Review Of On (Writing) Families: Autoethnographies Of Presence And Absence, Love And Loss, Edited By Jonathan Wyatt And Tony E. Adams, Jessica Smartt Gullion

The Qualitative Report

In this essay, I offer a review of the book, On (Writing) Families: Autoethnographies of Presence and Absence, Love and Loss, edited by Jonathan Wyatt and Tony E. Adams. An important contribution to the field of authethnography, this book will appeal not only to scholars of family and of qualitative inquiry, but also to people struggling to find meaning in the crazy complexities of family relationships.

Bjc Today

BJC Today

No abstract provided.

Sequencing, Identification And Mapping Of Primed L1 Elements (Simple) Reveals Significant Variation In Full Length L1 Elements Between Individuals, Vincent A. Streva, Vallmer E. Jordan, Sara Linker, Dale J. Hedges, Mark A. Batzer, Prescott L. Deininger

Faculty Publications

© Streva et al. Background: There are over a half a million copies of L1 retroelements in the human genome which are responsible for as much as 0.5% of new human genetic diseases. Most new L1 inserts arise from young source elements that are polymorphic in the human genome. Highly active polymorphic "hot" L1 source elements have been shown to be capable of extremely high levels of mobilization and result in numerous instances of disease. Additionally, hot polymorphic L1s have been described to be highly active within numerous cancer genomes. These hot L1s result in mutagenesis by insertion of new …

Probabilistic Records Linkage Using Historical Canadian Censuses And Vital Statistics, Stacey A. Hallman

Population Change and Lifecourse Strategic Knowledge Cluster Conferences

Poster Presentation

This poster presents the results of a records linkage project to analyze potential mortality risks for young adults in Ontario during the 1918 influenza epidemic. With a team of research assistants at Western University, McMaster University, and the Université de Montréal, death records were linked to birth records in order to determine exact date of birth for the calculation of exact age at death. This date of birth is then compared to age as listed on other historical documents, such as the 1901 and 1911 Canadian censuses. The family environment (or living situation) was transcribed for each individual …

Melding Data Collection Methodology With Community Assistance: Benefits To Both Researchers And The Indigenous Groups They Study, Douglas S. London

Journal of Ecological Anthropology

I present a description of a model of melding data collection with community aid in the form of health educator training that emerged in the process of research collaboration during 2009-2011 with the Kawymeno Waorani foragers of Amazonian Ecuador. Some guidelines are suggested as to how benefits to both parties might be achieved when collecting data with indigenous populations. In this article I describe some of the advantages and pitfalls of melding data collection and community aid with research when collaborating with vulnerable indigenous groups.

First National Kidney Graft Survival Study Of Hiv-Positive Recipients, Jeff Hansen

Browse All News

No abstract provided.

Informal Child Care And Adolescent Psychological Well-Being: Hong Kong’S “Children Of 1997” Birth Cohort, Cherry Y. Leung, Gabriel M. Leung, Mary Schooling

Publications and Research

Background
Informal child care (child care by untrained family members, relatives or employees in the home) in Western populations is often associated with poorer psychological well-being, which may be confounded by socioeconomic position. We examined the association of informal child care, common in non-Western settings, with adolescent psychological wellbeing, using Hong Kong’s Chinese “Children of 1997” birth cohort.

Methods
Multivariable linear regression was used to examine the adjusted associations of informal child care (at 0.5, 3, 5 and 11 years) with parent-reported Rutter score for child behavior at 11 years, self-reported Culture-Free Self-Esteem Inventories score at 11 years and selfreported …

Direct Detection Of Fungal Siderophores On Bats With White-Nose Syndrome Via Fluorescence Microscopy-Guided Ambient Ionization Mass Spectrometry, Samantha J. Mascuch, Wilna J. Moree, Cheng-Chih Hsu

KIP Articles

White-nose syndrome (WNS) caused by the pathogenic fungus Pseudogymnoascus destructans is decimating the populations of several hibernating North American bat species. Little is known about the molecular interplay between pathogen and host in this disease. Fluorescence microscopy ambient ionization mass spectrometry was used to generate metabolic profiles from the wings of both healthy and diseased bats of the genus Myotis. Fungal siderophores, molecules that scavenge iron from the environment, were detected on the wings of bats with WNS, but not on healthy bats. This work is among the first examples in which microbial molecules are directly detected from an infected …

Best Practice Guidelines For Uncomplicated Urinary Tract Infections To Reduce Rates Of Antibiotic Resistance: A Ce Module For Clinicians, Romina Lo-Montano

Dissertations

Uncomplicated urinary tract infections (UTIs) accounted for over 8 million visits to ambulatory clinics. The annual cost of evaluation and treatment for uncomplicated UTIs on the health care system is estimated to be approximately $2.14 billion (Barber, et al., 2013). The improper treatment of patients with uncomplicated UTIs will result in increased healthcare expenditures, increase exposure of patients to potential side effects and an increase in antibiotic resistance. This capstone projected addressed the problem of non-adherence to evidence-based guidelines to treat uncomplicated UTI in women, among primary care providers. The purpose of this capstone project was to develop an evidence-based …

Household Demography And Early Childhood Mortality In A Rice-Farming Village In Northern Laos, Shinsuke Tomita, Daniel M. Parker, Julia Jennings, James Wood

Anthropology Faculty Scholarship

This paper extends Alexandr Chayanov’s model of changing household demography (specifically the ratio of food consumers to food producers) and its influence on agricultural behavior so that it includes possible adverse effects of a rising ratio on nutritional status and early childhood mortality within the household. We apply the model to 35 years’ worth of longitudinal demographic and economic data collected in the irrigated-rice growing village of Na Savang in northern Laos. When appropriate controls are included for other household variables, unobserved inter-household heterogeneity, and changes in local conditions and national policy over the study period, the analysis suggests that …

Solids Accumulation Rates Of Latrines At Rural Schools In Nimba County, Liberia, Caraline M. Murphy

USF Tampa Graduate Theses and Dissertations

Access to appropriate sanitation facilities as well as access to clean drinking water are considered fundamental human rights (Carr, 2001; Bjorklund & Sjodin, 2010), yet roughly 2.5 billion people on the planet lack access to an improved form of sanitation (WHO/UNICEF, 2014). Additionally, many entities responsible for emergency excreta management and sanitation management design guidelines, specifically solids accumulation rates in latrine pits, use rates that are 30-60 years old and may be established from dated knowledge on diet and lifestyle trends (Franceys et al., 1992; Harvey et al., 2002; Harvey, 2007). Using solids accumulation rates that are dated as well …

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1- Latest Version of South Dakota “One Health” Meetings Explores Tuberculosis in Humans and Cattle [Page] 2- Director's Message [Page] 2- Bailey Herd Health Conference Covers Connection Between Facilities and Animal Health [Page] 3- Essential Behind the Scenes Work: Accounting and Human Resources Staff at the VBS and ADRDL [Page] 3- New Hires at South Dakota ADRDL [Page] 4- Dairy Animal and Product Testing Services at the ADRDL [Page] 6- Video Production Highlights ADRDL Value to Animals and Citizens [Page] 6- Bovine Respiratory Coronavirus Testing [Page] 6- Dr. Hennings Named to AAVLD Executive Board [Page] 6- Equine Well-Being Survey: …