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Cytokine Dynamics, Diagnosis And Treatment Of Neuroinflammation In Chronic Constriction Injury Rat Model, Kiran Vasudeva

Electronic Theses and Dissertations

Chronic neuropathic pain is a serious, worldwide health problem leading to life-long treatment and the possibility of significant disability. In this study, neuropathic pain was modeled using the chronic constriction injury (CCI) in rats. The CCI rats exhibited hypersensitivity (typical neuropathic pain symptom) to mechanical stimulation of the affected paw 11 days post-surgery, at a time when sham surgery animals did not. It is known that immune cells play a role in the development of neuropathic pain and to further explore the relationship between neuropathic pain and immune cells, we hypothesized that the infiltration of immune cells into the affected …

The Transmuted Exponentiated Generalized-G Family Of Distributions, Haitham M. Yousof, Ahmed Z. Afify, Morad Alizadeh, Nadeem Shafique Butt, Gholamhossein Hamedani

Mathematics, Statistics and Computer Science Faculty Research and Publications

We introduce a new class of continuous distributions called the transmuted exponentiated generalized-G family which extends the exponentiated generalized-G class introduced by Cordeiro et al. (2013). We provide some special models for the new family. Some of its mathematical properties including explicit expressions for the ordinary and incomplete moments, generating function, Rényi and Shannon entropies, order statistics and probability weighted moments are derived. The estimation of the model parameters is performed by maximum likelihood. The flexibility of the proposed family is illustrated by means of an applications to real dataset.

A High-Content Multiplexed Screening Platform For The Evaluation And Manipulation Of Force And Fatigue Of Adult Derived Skeletal Muscle Myotubes In Defined Serum-Free Medium, Christopher Mcaleer

Electronic Theses and Dissertations

The overall focus of this project has two parts: First, was to develop a protocol utilizing serum-free media formulations and defined plating and culture techniques to create functional in vitro myotubes derived from adult skeletal muscle satellite cells. The second was to manipulate the inherent muscle parameters such as force output and fatigue of these myotubes by employing exercise regimes or by small molecule application. The importance of serum-free medium use for in vitro cultures is becoming increasingly important in creating functional systems that can be validated for drug testing by the Food and Drug Administration (FDA). Also, the study …

Spatial Distribution And Sociodemographic Composition Of High Grade Cervical Lesion Clusters In Connecticut 2008-2013, Aref Senno

Public Health Theses

Introduction: HPV is necessary for the development of invasive cervical cancer. There were 530,000 cases and 275,000 deaths attributable to cervical cancer in 2008. Since the introduction of HPV vaccines in 2006, the incidence of high grade cervical lesions (HGCL)—precancerous lesions which may develop into cervical cancer—has decreased significantly. Some changing sociodemographic trends in disease burden have been previously reported since 2008.

Objectives: The objectives of this analysis were to identify regions of Connecticut with high rates of HGCLs and to describe how trends of disease changed over time in the post-vaccine era.

Methods: This is a descriptive analysis of …

The Desperate Need To Include Pregnant Women In Clinical Research: Proposed Recommendations To Increase Enrollment Of Pregnant Women In Research, Julia Belton

Student Works

No abstract provided.

Observing, Understanding, And Manipulating Biological Membranes, Ian Mc Cabe

Dissertations - ALL

The phospholipid bilayer is one of the hallmarks of eukaryotic life. This complicated two dimensionally fluid surface is composed of a double layer of lipids which have a region that is hydrophobic and a region that is hydrophilic. The lipid bilayer membranes of a cell act as a barrier that distinguishes the cells and the organelles interiors from the outside environment. In order for the cell to be able to effectively communicate across these impermeable barriers they have evolved many intricate systems of lipid and protein interaction that serve to transmit information from one side of the membrane to the …

The Inclusion Of Engineering Design Into The High School Biology Curriculum, Cody D. Lakose

Graduate Research Papers

The purpose of this project is to develop engineering-based lessons for a life science course,i.e. biology, in order to meet the NGSS standards as well as increase student interest in engineering by incorporating the principles of engineering design into the traditional science classroom.

Lynn - 2015 Annual Edition, Lynn University

Lynn Magazine / LYNN

Main Stories:

• College reimagined
• A sanctuary for all
• Perpers help Lynn stay focused on the future

Evaluation Of In Vitro Anti-Inflammatory, Anti-Diabetic And Anti-Lipogenic Activity Of Natural Polyphenolic Extracts And Their Pure Constituents, Pragati P. Nahar

Open Access Dissertations

The long-term goals and objectives of our group are to identify bioactive natural products relevant to human disease prevention and health promotion. To attain this goal, the objective of this thesis was to demonstrate that bioactive polyphenol-rich extracts or preparations exert positive health benefits beyond basic nutrition, thus impacting overall health and wellness. We believe that this project has immense scientific merit from a human health perspective and will be of great public impact given that consumers are seeking natural and organic choices for improving health. In this investigation, we have focused our attention on investigating the biological activities of …

Development Of Dual-Wavelength And Pulsed Fiber Lasers Based On Thulium-Doped Fibers / Salam Mahdi Azooz, Salam Mahdi Azooz

Student Works (2010-2019)

A growing interest on lasers operating in the eye-safe two micron spectral region especially on dual-wavelength and pulse operations for a variety of applications motivated this PhD work. The scope of this thesis focuses on the development of new fiber lasers, which incorporates Thulium fiber as either gain medium or saturable absorber. At first, all-fiber dual-wavelength 2 μm fiber lasers are demonstrated based on three simple approaches; spatial filtering effect, inline microfiber Mach Zehnder Interferometer (MMZI) and multimode interference effect. The use of MMZI in the 2 μm laser cavity, for instance, successfully generates two simultaneous laser lines at 1952.93 …

Bioactive 4-Phenylcoumarins From Mesua Elegans And Mesua Kunstleri / Chan Gomathi, Gomathi Chan

Student Works (2010-2019)

Phytochemical analyses on the bark of two Clusiaceae plants, Mesua elegans and Mesua kunstleri, were carried out. Twenty-four compounds were successfully isolated using several chromatographic techniques; column chromatography (CC), thin layer chromatography (TLC), preparative thin layer chromatography (PTLC), centrifugal partition chromatography (CPC) and high performance liquid chromatography (HPLC). The structures of the isolated compounds were elucidated using various spectroscopic methods; 1D-NMR (1H, 13C and DEPT), 2D-NMR (COSY, HSQC, HMBC and NOESY), UV, IR, HRESIMS and by comparison with data from the literature. A total of twenty-two compounds were isolated from M. elegans, namely mammea A/BA 28, 5,7-dihydroxy-8-(2-methylbutanoyl)-6-[(E)-3,7-dimethylocta-2,6-dienyl]-4- phenyl-2H-chromen-2-one 47, 5,7-dihydroxy-8-(3-methylbutanoyl)-6-[(E)-3,7- …

Stress’ Impact Of Supervisory Styles In U.S. Navy Reservists In Central New York During The Military Fiscal Year 2013-2014, Miguel A. Reyes-Mariano

Public Administration Master’s Projects

Recent studies on stress have paid close attention to the outcomes of global trends like the wars on terrorism. In this convulsed world of the 21st Century, civilians and military are exposed to a myriad of drawbacks, and everyone has to manage to carry out their lives and duties. The resulting work-related stress is present in a significant number of complaints in medical centers and hospitals; public, private or military. Although the United States Ready Reserve has not received much attention in the past, they are getting it now, given its more active interactions on foreign and domestic public policies. …

Issue 4

The Synapse: Intercollegiate science magazine

No abstract provided.

The Dimerization Kinetics Of Ncad12 With A Mutated Cis-X-Pro Bond, Victoria Mcclearn

Honors Theses

VICTORIA RAE McCLEARN: DIMERIZATION KINECTICS OF NCAD12 P16A (Under the direction Dr. Susan Pedigo) Calcium binding causes a conformational change into a strand swapped dimer for the cadherins. However, regardless of the sequence similarity for Epithelial Cadherin (ECAD) and Neural Cadherin (NCAD), they respond to the presence of calcium differently. For NCAD, the rate of assembly and disassembly depends upon the presence of calcium, whereas for ECAD the rate of exchange is independent of calcium. Studies into a kinetic model to explain the differences between the rates have been recently conducted. It is believed that ECAD uses an X-dimer intermediate …

Synthetic Oligodeoxynucleotide Purification Via Catching By Polymerization, Suntara Fueangfung

Dissertations, Master's Theses and Master's Reports - Open

Large quantities of pure synthetic oligodeoxynucleotides (ODNs) are important for preclinical research, drug development, and biological studies. These ODNs are synthesized on an automated synthesizer. It is inevitable that the crude ODN product contains failure sequences which are not easily removed because they have the same properties as the full length ODNs. Current ODN purification methods such as polyacrylamide gel electrophoresis (PAGE), reversed-phase high performance liquid chromatography (RP HPLC), anion exchange HPLC, and affinity purification can remove those impurities. However, they are not suitable for large scale purification due to the expensive aspects associated with instrumentation, solvent demand, and high …

The Fanned Flames Of Discontent: A Solidarity-Inspired History Of The Identity/Ideology, Cultural History, And Rhetorical Strategies Of The Wobblies During The 1916 Minnesota Iron Ore Strike, Gary Kaunonen

Dissertations, Master's Theses and Master's Reports - Open

Rooted in critical scholarship this dissertation is an interdisciplinary study, which contends that having a history is a basic human right. Advocating a newly conceived and termed, Solidarity-inspired History framework/practice perspective, the dissertation argues for and then delivers a restorative voice to working-class historical actors during the 1916 Minnesota Iron Ore Strike. Utilizing an interdisciplinary methodological framework the dissertation combines research methods from the Humanities and the Social Sciences to form a working-class history that is a corrective to standardized studies of labor in the late 19th and early 20th centuries. Oftentimes class interests and power relationships determine the dominant …

Oxidative Stress Biomarkers And Incidence Of Postoperative Atrial Fibrillation In The Omega‐3 Fatty Acids For Prevention Of Postoperative Atrial Fibrillation (Opera) Trial, Ralph J. Damiano, Joann Marsala, Et Al

Open Access Publications

No abstract provided.

Synechococcus Elongatus Utex 2973, A Fast Growing Cyanobacterial Chassis For Biosynthesis Using Light And Co2, Jingjie Yu, Michelle Liberton, Paul F. Cliften, Richard D. Head, Jon M. Jacobs, Richard D. Smith, David W. Koppenaal, Jerry J. Brand, Himadri B. Pakrasi

Open Access Publications

No abstract provided.

Thermal Variation, Thermal Extremes And The Physiological Performance Of Individuals, W. Wesley Dowd, Felicia A. King, Mark W. Denny

Biology Faculty Works

In this review we consider how small-scale temporal and spatial variation in body temperature, and biochemical/physiological variation among individuals, affect the prediction of organisms’ performance in nature. For ‘normal’ body temperatures – benign temperatures near the species’ mean – thermal biology traditionally uses performance curves to describe how physiological capabilities vary with temperature. However, these curves, which are typically measured under static laboratory conditions, can yield incomplete or inaccurate predictions of how organisms respond to natural patterns of temperature variation. For example, scale transition theory predicts that, in a variable environment, peak average performance is lower and occurs at a …

Mercury Methylation Potentials In Sediments An Ancient Cypress Wetland Using Species-Specific Isotope Dilution-Gc-Icp-Ms: Analytical Method Development And The Effect Of Sorbent Amendments, Derek Davin Bussan

Electronic Theses and Dissertations

A novel method was developed to measure mercury (Hg) directly in environmental solids using inductively coupled plasma mass spectrometry (ICP-MS) with online sample ashing. The method combines a direct mercury analyzer (DMA) that is based on sample combustion, Hg pre-concentration by amalgamation with gold, and atomic absorption spectrometry (AAS), with a high resolution ICP-MS. Unlike AAS, the ICP-MS is capable of individual isotope measurements allowing for accurate quantitation by isotope dilution mass spectrometry (IDMS) and for isotope tracer experiments. The method was validated using certified reference materials including sediment (NIST 2709), leaves (NIST 1547), and fish-muscle (DORM-3), with recoveries within …

Primer Efficacy In The Dna Barcoding Of Spiders, Rhennetta Jo Bork

Honors Program Theses

DNA barcoding is the process of amplifying a 650 base pair segment of the sequence of the mitochondrial gene cytochrome c oxidase (COI), and amplifying this gene with a polymerase chain reaction (PCR). It is used to help identify and distinguish animal species and also to help determine genetic differences in species. DNA barcoding can be especially useful when working with spiders since they tend to be very small and hard to distinguish. However, achieving a DNA barcode can be difficult and thus techniques to improve the method of DNA barcoding can be helpful. This research looked into the different …

The Increasing Weight Of Regulation: Countries Combat The Global Obesity Epidemic, Emily Whelan Parento, Allyn L. Taylor, Laura A. Schmidt

McGeorge School of Law Scholarly Articles

Obesity is a global epidemic, exacting an enormous human and economic toll. In the absence of a comprehensive global governance strategy, states have increasingly employed a wide array of legal strategies targeting the drivers of obesity. This Article identifies recent global trends in obesity-related legislation and makes the normative case for an updated global governance strategy. National governments have responded to the epidemic both by strengthening traditional interventions and by developing novel legislative strategies. This response consists of nine important trends: (1) strengthened and tailored tax measures; (2) broadened use of counter-advertising and health campaigns; (3) expanded food labeling; (4) …

A Novel Function For Fancj Helicase In Microsatellite Stabilization During Replication Stress, Joanna Barthelemy

Browse all Theses and Dissertations

Noncanonical DNA structure-forming sequences, such as hairpin structures, stall replication forks in vivo, expand or contract during DNA replication, and colocalize with chromosome fragile sites. Since the frequency of hairpin formation is higher than the frequency of expansion or contraction, the cell may possess mechanisms to resolve hairpin structures prior to replication fork stalling. One possible activity for hairpin resolution is the FANCJ DNA helicase, known to unwind noncanoncial DNA structures in vitro. Indeed, mutations or loss of any of the nineteen FANC genes, including FANCJ, leads to genome instability and the cancer-prone syndrome Fanconi anemia (FA). To test whether …

A Far-Field Radio Frequency Experimental Exposure System And 31 Day Exposure Experiment On Understanding Mice, Jared William Hansen

Electrical and Computer Engineering

Many studies have been performed on exploring the effects of radio frequency energy on biological function in vivo. In particular, gene expression results have been inconclusive due, in part, to a lack of a standardized experimental procedure. This thesis describes a new far field RF exposure system for unrestrained murine models that reduces experimental error and a 31 day experiment using mice in vivo. The experiment uses whole body exposure to continuous RF energy at 2.45 GHz on unrestrained, in vivo mice. Using RNA-seq to analyze the entire murine genome, the data is statistically analyzed using combinations and empirical p-values. …

Aphid Interactions With Environmental Variation In The Field And Lab, Erin Leigh Mclean

Entomology

A basic tenet of ecology is that organisms are affected by both abiotic factors and other organisms; therefore, there is value in understanding interactions in our changing world. Aphids are model organisms for questions regarding many interactions. I explored three aphid-centered studies. (1) Rearing aphids in a certain temperature changed their response to exposure to different temperatures. Short term exposure to warmer temperatures increased fecundity, but being raised in higher temperatures lowered fecundity across treatments. (2) Feeding on aphids by lady beetles in the lab was measured after exposure to varying temperatures with or without prey. Warming without prey was …

A Comparison Of Two Low-Stakes Methods For Administering A Program-Level Biology Concept Assessment, Brian A. Couch, Jennifer K. Knight

School of Biological Sciences: Faculty Publications

Concept assessments are used commonly in undergraduate science courses to assess student learning and diagnose areas of student difficulty. While most concept assessments align with the content of individual courses or course topics, some concept assessments have been developed for use at the programmatic level to gauge student progress and achievement over a series of courses or an entire major. The broad scope of a program-level assessment, which exceeds the content of any single course, creates several test administration issues, including finding a suitable time for students to take the assessment and adequately incentivizing student participation. These logistical considerations must …

Green And Energy-Efficient Methods For The Production Of Metallic Nanoparticles, Mitra Naghdi, Mehrdad Taheran, Satinder K. Brar, M. Verma, R. Y. Surampalli, J. R. Valero

Department of Civil and Environmental Engineering: Faculty Publications

In the last decade, researchers paid great attention to the concept of “Green Chemistry”, which aims at development of efficient methods for the synthesis of nanoparticles (NPs) in terms of the least possible impact on human life and environment. Generally, several reagents including precursors, reducing agents, stabilizing agents and solvents are used for the production of NPs and in some cases, energy is needed to reach the optimum temperature for reduction. Therefore, to develop a green approach, researchers had the opportunity to investigate eco-friendly reagents and new energy transfer techniques. In order to substitute the harmful reagents with green ones, …

Using Computer Simulations As A Pre-Training Activity In A Hands-On Lab To Help Community College Students Improve Their Understanding Of Physics, Blanca Pineda

Doctoral Dissertations

The purpose of this study was to investigate the effectiveness of using computer simulations as a pre-training activity to a hands-on lab to improve students’ understanding of induction topics in physics. The computer simulation activity was compared to an overview presentation. Conceptual understanding and spatial ability were measured. A two-group descriptive repeated measures design was implemented with a convenience sample of 35 community college physics students in the Bay Area. Participants were randomly assigned to a simulation group (n = 17) or a presentation group (n = 18). A 30-item spatial ability assessment was given to all participants one week …

Introduction To The Modeling And Analysis Of Complex Systems, Hiroki Sayama

Milne Open Textbooks

Keep up to date on Introduction to Modeling and Analysis of Complex Systems at http://bingweb.binghamton.edu/~sayama/textbook/!

Introduction to the Modeling and Analysis of Complex Systems introduces students to mathematical/computational modeling and analysis developed in the emerging interdisciplinary field of Complex Systems Science. Complex systems are systems made of a large number of microscopic components interacting with each other in nontrivial ways. Many real-world systems can be understood as complex systems, where critically important information resides in the relationships between the parts and not necessarily within the parts themselves. This textbook offers an accessible yet technically-oriented introduction to the modeling and …

Cdna Cloning, Molecular Characterization, And Expression Analyses Of Two Novel Porcine Arrdc Genes-Arrdc1 And Arrdc, Hailong Huo, Pei Wang, Shuyan Wang, Yongwang Miao, Hongjiang Wei, Yongyun Zhang, Qiaoling Zhang, Fuquan Li, Lixian Liu, Rui Wang, Weizhen Li, Yue Zhao, Jinlong Huo, Heng Xiao

Turkish Journal of Biology

Arrestin-domain-containing proteins belong to the arrestin family, embers of which are important for regulating signal transduction within cells. In this study, two ARRDC genes (ARRDC1 and ARRDC5) of Diannan small-ear pigs were amplified by reverse transcription polymerase chain reaction and cloned for the first time (accession nos.: KF589202 and KF589203). The full-length coding sequence of ARRDC1 was 1284 nucleotides in length (molecular weight, 45.6 kDa; pI, 7.61) and encoded 427 amino acids. The ARRDC5CDS was 1014 nucleotides in length (molecular weight, 37.5 kDa; pI, 7.08) and encoded 337 amino acids. Sequence homology analysis revealed that the amino acid sequences of …