Digital Commons Network^™

Investigating The Role Of Bptf In Immunoediting In Breast Cancer And Melanoma, Kristen N. Peterson

Theses and Dissertations

In this study, we explore the effects of NURF depletion on the growth of tumors in immune-competent mice. NURF depletion in tumors results in reduced tumor growth in immune-competent mice, suggesting enhanced anti-tumor immunity. Analysis of the tumor microenvironment by flow cytometry revealed a significantly elevated CD8 and progressively elevated activated CD8 phenotype in Bptf KD tumors, possibly contributing to the increase in cell death and decrease in tumor weight observed. Examination of antigen presentation was evaluated using the OT-1 and Pmel-17 models, though no significant difference in cytotoxicity was observed as measured by LDH and/or IFNγ assays. This indicates …

Polycultural Interactions: Fuzzy Identity, Xi Lu

Theses and Dissertations

“Robin D.G. Kelley coined the term polyculturalism as an alternative to multiculturalism, ‘since the latter often implies that cultures are fixed, discrete entities that exist side by side—a kind of zoological approach to culture.’ ”

Polyculturalism assumes the whole world’s cultures are interactive and fluid instead of independent and static, and individuals’ relationship to cultures are complex and cannot be categorized. Yet an individual constitutes multiple cultures, and individual identity embraces the various forms of culture in all aspects of one’s life.

My research examines how polyculturalism affects aspects of communications among people who hold a multiplicity of voices. It …

Applications Of Stability Analysis To Nonlinear Discrete Dynamical Systems Modeling Interactions, Jonathan L. Hughes

Theses and Dissertations

Many of the phenomena studied in the natural and social sciences are governed by processes which are discrete and nonlinear in nature, while the most highly developed and commonly used mathematical models are linear and continuous. There are significant differences between the discrete and the continuous, the nonlinear and the linear cases, and the development of mathematical models which exhibit the discrete, nonlinear properties occurring in nature and society is critical to future scientific progress. This thesis presents the basic theory of discrete dynamical systems and stability analysis and explores several applications of this theory to nonlinear systems which model …

Prediction Of Protein-Protein Interactions In Escherichia Coli From Experimental Data In Treponema Pallidum, Marco A. Abreu

Theses and Dissertations

Protein – Protein interactions (PPIs) are thought to be conserved between species, although this has not been systematically investigated. This problem was explored in Escherichia coli from experimental data in Treponema pallidum by predicting PPIs, focusing on protein domains of little or unknown function. The comparison of T. pallidum to a model organism such as E. coli can not only reveal additional data about T. pallidum but also reveals how E. coli is similar to this distantly related, obligate parasite. A set of novel T. pallidum interactions, enriched for proteins of unknown function, were the basis of over 23,000 predicted …

Cute As A Button, Marta R. Finkelstein

Theses and Dissertations

Cute As A Button explores powerlessness, vulnerability, illness and addiction all wrapped up in tender buttons and a cute, cuddly creature. Using animation, sculpture, sound and an intimate space, I surround the viewer in a saccharine nightmare, one that references the dark underbelly of the cute and the sweet. The visual and aural elements are representative of the psychological and emotional states of powerlessness, which are overcome by the act of making and exploring a medium over which I can have complete control.

The Role Of Adam10, Adam17, And Spag6 In Humoral Immunity And Secondary Lymphoid Tissue Architecture, Lauren Folgosa Cooley

Theses and Dissertations

ADAM10, ADAM17, and SPAG6 contribute significantly to humoral immunity and secondary lymphoid tissue architecture. ADAM10 and ADAM17 are two closely related zinc-metalloproteinases. Through cleavage of their ligands CD23 and TNF, respectively, they greatly influence IgE production and secondary lymphoid tissue architecture maintenance. Th1 prone WT strains initially exhibit increased ADAM17 and TNF yet reduced ADAM10 relative to Th2 prone WT strains. In the absence of B cell ADAM10, a compensatory increase in ADAM17 and TNF cleavage is noted only in Th1 prone C57Bl/6, not Th2 prone Balb/c. B cell TNF homeostasis is important for maintaining secondary lymphoid tissue architecture. We …

Rituals, Our Past, Present & Future. Glimpses Of Islamic Enrichment, Othman Mohamed Khunji Mr

Theses and Dissertations

A Muslim should be encouraged to comprehend the benefit

and value behind every aspect of Islamic practice and wisdom,

and not just practice their religion because they were told to do

so. The products proposed in this thesis aim to achieve this by

inviting and encouraging a Muslim to practice The Five Pillars

of Islam while comprehending their value through the use of

modern means such as Arduino technology, 3D printing and

visual computing programing.

I am provoked by the fact that the circle of Gulf-region Muslims

I’m surrounded by, and have been exposed to since childhood,

belong to one …

The Orphanage Of Things: A Narrative Of Abandonment, Malaz Elgemiabby

Theses and Dissertations

In Sudan, 110 babies are abandoned in the streets of Khartoum every month. The majority of abandoned children are born out of wedlock. Young women with illegitimate pregnancies are often ostracized by their families and society, and the lack of emotional, financial and legal support has led many to take desperate measures, including the abandonment of their children. Relinquishing mothers exist like ghosts in Sudanese society. The only evidence of the mother’s experience is her anonymous, abandoned child. In order to understand and examine this phenomenon, I used ethnographic performance art informed by design research practice (Performative Research Design). I …

Thank You For A Lovely Time, Kelley Mj Morrison

Theses and Dissertations

Stemming from my own fixation on trying to understand the actions, decisions and thought processes of those around me my work explores the difference between internal and external lives. I construct “brain spaces”; whether my own or someone else’s I strive to understand the decisions they have made and the stories we are a part of. I combine thoughts, feeling and sentiments which I have collected over my life to make objects that act as intermediaries, allowing others to imagine stepping into a mind other than their own. I attempt to bring the landscape of the mind into the physical …

Inhibition Of Mtor Signaling Protects Against Myocardial Reperfusion Injury, Acute Myocardial Infarction, Scott M. Filippone

Theses and Dissertations

Acute myocardial infarction (AMI) is the leading cause of death worldwide. Currently, the best method of treating cardiac ischemia is early reperfusion which, itself, induces myocardial damage. The mTOR complex is a key regulator of cardioprotection against cell stressors. We hypothesized that reperfusion therapy with Rapamycin, a potent mTOR inhibitor, would reduce infarct size in adult mouse hearts. Rapamycin was administered at the onset of reperfusion following 30 min in situ LAD ligation. After 24 hours of reperfusion, myocardial infarct size and apoptosis were significantly reduced in rapamycin-treated mice compared to control. Rapamycin inhibited pro-apoptotic protein Bax and phosphorylation of …

Cyclic Gmp: A Satiety Signal In C. Elegans, Ji S. Park

Theses and Dissertations

Appetite control and satiety mechanisms help animals maintain energy homeostasis; however, these mechanisms can be misregulated, leading to overweight and obesity. Caenorhabditis elegans is an excellent model system to study appetite and satiety because of its conserved behavioral aspects of satiety and conserved molecular mechanisms. ASI senses nutrition and its activity is required for the behavioral state of satiety quiescence. The purpose of this thesis project was to elucidate the function of cGMP signaling in ASI by looking at behavioral effects from the pharmacological use of sildenafil (Viagra), a PDE inhibitor, and the effects on ASI activation from mutating guanylyl …

Postcession, Evan D. Pomerantz

Theses and Dissertations

This is a series of daily writings. Each day consists of a new topic and is closed at the end of the day. The ideas presented are philosophical, humorous, rambling, lamentations, incantations, doubt-ridden, aesthetic pep talks which combine into an affective representation of my studio practice’s becoming. There will be little congruency, some stories, and a lot of parallels because that is who I am.

Tracing Motion, Stephanie M. Clark

Theses and Dissertations

This document explores the use of motion within design to defamiliarize a message. The objective is to expand a viewer’s level of understanding through prolonged perception. I experiment with this idea using present-day tools which afford my own movement during the capturing process to create various visual interpretations of motion. I look to László Moholy-Nagy, Aleksandr Rodchenko and Eadweard Muybridge who explored the use of the camera, the new technology of their day, to understand its potential to create a new visual language. They believed the lens of the camera was the eye of the future—and the public’s exposure to …

Virsa: The Contemporary Value Chain, Samreen Zahra Mrs

Theses and Dissertations

"Handicraft" means a useful or decorative object made by a craftsman who has direct control over all stages of production. Handicrafts have always had a greater value, an identity of their own that is reflective of their place, culture and materials, as well as a sense of belonging to a particular place and time. With the ever-growing mass production that followed the Industrial Revolution, we lost those crafts to multiple reasons: one being cheaper, industrial-made products reducing the demand for handicrafts, and another being a shift in consumer tastes. Most craftspeople hardly earn enough to survive and fulfill their basic …

Reconstructing A Science Fiction Autobiography, Jason R. Rood

Theses and Dissertations

This is a collection of essays revolving around concepts pertinent to my current visual arts practice, centering on a deconstruction of personal narrative mythologies. What is generated is a web of connection that spans Narrative Modes, Science Fiction, the use of Lines and Drawing, the Digital and Space and Time. Through this interweaving of topics, I am beginning the process of rebuilding the structure of a personal story for the future.

The Object Inbetween, Lucy L. Derickson

Theses and Dissertations

My mission is to bring attention to the unusual, undefined, and fluid connections we have to objects through various avenues of experience. Things, objects, and devices have shaped our culture, environment, and personal philosophies. We live, grow, learn, and mourn with objects. We worship, sympathize, and relate to objects. But what I am most interested in is a human tendency to retreat to objects, allowing them to mediate experiences of friendship and intimacy between people.

During my graduate studies, I have been evaluating my personal and professional connections with others in order to understand how technology such as smart phones …

Development Of Novel Chimeric Epitope Based Diagnostic Antigens And Vaccines For Lyme Disease, Lee D. Oliver Jr.

Theses and Dissertations

Lyme disease (LD) is the leading arthropod-borne disease in North America with 300-600,000 cases each year. There are currently no approved human LD vaccines. Outer surface protein C (OspC) has emerged as a leading vaccine candidate and an attractive diagnostic marker due to its antigenicity and expression early in infection. Several chimeric, epitope based OspC derived proteins were generated. The constructs were found to be highly immunogenic in mice and vaccination induced complement-dependent bactericidal antibodies. These results suggest that a broadly protective polyvalent OspC epitope based vaccine can be produced. Currently, LD diagnostic approaches are unreliable and unable to differentiate …

Polysaccharide-Based Shear Thinning Hydrogels For Three-Dimensional Cell Culture, Vasudha Surampudi

Theses and Dissertations

The recreation of the complicated tissue microenvironment is essential to reduce the gap between in vitro and in vivo research. Polysaccharide-based hydrogels form excellent scaffolds to allow for three-dimensional cell culture owing to the favorable properties such as capability to absorb large amount of water when immersed in biological fluids, ability to form “smart hydrogels” by being shear-thinning and thixotropic, and eliciting minimum immunological response from the host. In this study, the biodegradable shear-thinning polysaccharide, gellan-gum based hydrogel was investigated for the conditions and concentrations in which it can be applied for the adhesion, propagation and assembly of different mammalian …

Reconnect: Designing To Touch, Dana Seros Rohani

Theses and Dissertations

Our everyday lives are surrounded by gadgets and digital devices that help us perform our daily chores with ease and efficiency. However, these digital devices can also separate us from what we should do ourselves. Although children who are exposed to high levels of technology might become attuned to the latest and the best gadgets, they might not learn to use their physical abilities. Another implication of a child’s over dependence on technology is that parental interactions such as encouragement, tutoring and reinforcement are provided by gadgets rather than living, breathing parents. Research done by Padma Ravichandran and Brandel France …

Limelight & Indigestion, Joshua D. Thorud

Theses and Dissertations

This thesis chronicles the progression toward and creation of my thesis show, Limelight & Indigestion, as well as the cultural, technological and artistic influences and discussions that underpin the works therein. The show is an exploration of celebrity, mass media, and the nature of the desire for fame. I hope to situate my work through an investigation of topics such as Hollywood and the use of green screens and associated technology, our physical and ideological connection to cinema, the absurd in cinema history, and the complex nature of media digestion.

Creating Place For A Placeless Generation, Laura Wilson

Theses and Dissertations

Making up one quarter of the current United States population, some 80 million Generation Y-ers are changing the ways in which we live, work and play. Dubbed “Millennials” this population is comprised of those individuals born between 1980 and 2000. This generation is the first to have been raised with cell phones, the internet, and reality television. The “Selfie” or “Me Generation” is snubbed for narcissism and an instant gratification attitude. Yet on the whole Millennials have progressive values, are well educated, are conscious of their health and are optimistic about the future despite coming of age during the Great …

New Patriarchies: A Turbulence Of Source And Subject, Stephen Fuller

Theses and Dissertations

Experiencing a turbulence of source and subject in the variable inversions and supports of one source to another--the wreck of the U-352, Carpeaux’s Ugolino and his Sons, a movie poster for J.A. Bayona’s The Impossible, and Cassiopeia mythology--these four sources as sons, in sacrifice to and surviving by way of “daddy” documentation, are here refigured to reenact and critique the patriarchally recreational, monumental, cinematic, and mythological infrastructures supporting the sources of this work and thereby serving to critique the newer patriarchies to which these sources and their subjectifications here seek to cross consumptively dead end. Following three public …

Z Axis Follies, Adam Collignon

Theses and Dissertations

This document will outline the nature of performance / object interaction, and the role of documentation in this interaction. In addition, I will explore the body / object as a site of multiplicities, the quality of simultaneity in appearance and apprehension of such multiplicities, and traverse the triumphs and travails of the body / object’s journey from one state of being to the next.

The Majlis Metamorphosis: Virtues Of Local Traditional Environmental Design In A Contemporary Context, Shaikha Almahmoud

Theses and Dissertations

In the Arabian Gulf countries, the majlis is a central part of the house. The most public space within it, the majlis represents the household’s occupants to society and its social and economic status. As the house reflects culture and civilization, so the family is understood as a micro-level society of individuals raised in its institutions. Hence, the house is a manifestation of family structure, religious beliefs, and individual needs and desires, reflecting the family’s economic, cultural, and social backgrounds and aspirations. The majlis offers a unique space in Arab societies, articulating cultural and social factors that directly impact identity …

Fallen/Lifted, Hillary Waters Fayle

Theses and Dissertations

I use found botanical material such as leaves, seedpods, and branches to explore human connection to the physical world. By combining these organic objects with the rich traditions of needlecraft, I bind nature and the human touch. Both tender and ruthless, this intricate stitch work communicates the idea that our relationship with the natural world is both tenuously fragile and infinitely complex.

The way I think about and make art mirrors the way I think about my life and how I walk through the world. What I do is about elevating details. It is about noticing cycles and connections. It …

Understanding The Impact Of Leadership And Organizational Culture On Nonprofit Employees’ Commitment And Turnover Intention, Nancy A. Toscano

Theses and Dissertations

Child and family nonprofit organizations are essential for the implementation of United States public policy in their role as service providers. Human service nonprofit organizations held approximately 20,000 government contracts, totaling more than $100 billion in 2009 (Boris, deLeon, Roeger, & Nikolva, 2010). Almost 33,000 human service nonprofit organizations contract with the government to deliver services (Boris, et al., 2010). The services provided by these organizations are critical to the lives of vulnerable American citizens. These organizations depend on committed employees to serve this group, carry out the mission, and reach organizational goals. Employees are nonprofit organizations’ greatest resource, investment, …

Cell Cycle Regulation Of Retinal Progenitors; A Role For The Nance-Horan Syndrome Protein In Retinogenesis, Paul J. Vorster

Theses and Dissertations

The Nance-Horan syndrome gene (NHS) plays a role in lens, eye and brain development. To date, the function of NHS remains unclear. Recent evidence showed that p53 isoform, Δ113p53, inhibits abnormal cell growth during organogenesis. We show that NHS is expressed in the retinas of Danio rerio and Xenopus tropicalis during key stages of retinogenesis, and that knockdown of the gene resulted in a small eye phenotype in both species. Initially, knockdown of nhsb in zebrafish had no visible defects at 24hpf. But examination of the retina at 48hpf, we see a marked difference in size compared to control embryos. …

A Comparison Of Obesity Interventions Using Energy Balance Models, Marcella Torres

Theses and Dissertations

An energy balance model of human metabolism developed by Hall et al. is extended to compare body composition outcomes among standard and proposed obesity interventions. Standard interventions include a drastic diet or a drastic diet with endurance training. Outcomes for these interventions are typically poor in clinical studies. Proposed interventions include a gradual diet and the addition of resistance training to preserve lean mass and metabolic rate. We see that resistance training, regardless of dietary strategy, achieves these goals. Finally, we observe that the optimal obesity intervention for continued maintenance of a healthy body composition following a diet includes a …

The Effects Of Nicotine Sequestration On The Dynamics Of Hyperparasitism In A Stage-Structured Model Of Manduca Sexta And Its Related Parasitoid Wasps, Mark P. Zimmerman

Theses and Dissertations

Two proposed models will be used to help answer a long observed question in the dynamics of \textit{Manduca sexta} and its related parasitoid wasps-Why is there a large difference in diversity in hyperparasitoid species between tobacco and other related plants such as tomato? Two stage structured differential equation models are presented. The first is a single patch model to study the changes in dynamics that occur between hosts, parasitoids, and hyperparasitoids as the amount of nicotine in the plant increases. The second is a two patch model that allows hyperparasitoids to choose between patches that are nicotine negative (i.e. tomato …

Wilson's "Does Altruism Exist? Cultures, Genes, And The Welfare Of Others" (Book Review), Lisa Cutforth-Anderson

The Christian Librarian

No abstract provided.