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A Comparison Of Management Strategies For The Federally Endangered Running Buffalo Clover (Trifolium Stoloniferum) On The Blue Grass Army Depot, Ky, Alexi David Dart-Padover

Online Theses and Dissertations

Running buffalo clover (Trifolium stoloniferum) is a federally endangered plant that appears to depend on habitat disturbance, although proposed management strategies such as cattle grazing, mowing, and herbicide application have never been compared in a controlled study. We evaluate the efficacy of these techniques on the Blue Grass Army Depot (BGAD) in Madison County, KY, where one of T. stoloniferum’s largest populations occurs. Fifty-nine patches of T. stoloniferum on the BGAD were treated annually between 2012 and 2014 with combinations of mowing and grass-specific herbicide. Patches of T. stoloniferum also were exposed to one of three types of cattle exposure …

Spatial And Temporal Characterization Of The Physicochemical Parameters And Phytoplankton Assemblages In Dodurga Reservoir (Sinop, Turkey), Seda Hasirci Mustak, Eli̇f Tezel Ersanli

Turkish Journal of Botany

Physicochemical parameters and phytoplankton seasonal variation were investigated between August 2010 and July 2011 in Dodurga Reservoir. A total of 35 phytoplankton taxa were identified. Phytoplankton samples collected from surface samples in late winter, spring, and early and mid-summer were found to be influenced by nitrate, dissolved oxygen, pH, temperature, and phosphate. However, early winter and autumn assemblages of the samples were influenced by total hardness, silica, water transparency, conductivity, organic matter, and oxidation reduction potential. The phytoplankton samples collected from different depths in early and mid-summer were found to be influenced by only temperature. Early and middle spring assemblages …

Metabolic And Molecular-Genetic Regulation Of Proline Signaling And Itscross-Talk With Major Effectors Mediates Abiotic Stress Tolerance In Plants, Aryadeep Roychoudhury, Aditya Banerjee, Vikramjit Lahiri

Turkish Journal of Botany

Proline (Pro) accumulation is a common response of several plant species to combat abiotic stresses. Under stress conditions, Pro acts as an excellent compatible solute in the plant system, participating in the alleviation of stress sensitivity. Though the metabolic pathways associated with Pro are well studied, parts of its regulatory cascades are still not properly known. It has also been conjectured that epigenetic modifications regulate Pro metabolism during abiotic stress. Apart from Pro, the plant abiotic stress responses are essentially mediated by multiple effectors. Hence, proper analysis of the cross-talks of Pro with the other components of the abiotic stress …

Recent Advances In Potato Genomics, Transcriptomics, And Transgenicsunder Drought And Heat Stresses: A Review, Emre Aksoy, Ufuk Demi̇rel, Zahi̇de Nesli̇han Öztürk, Sevgi̇ Çalişkan, Mehmet Emi̇n Çalişkan

Turkish Journal of Botany

Sustainable potato production practices are crucial for food security and social sustainability in the future since potato is a highly nutritious food and it is considered as one of the most promising crops to reduce human hunger and poverty in the world due to its high yield potential. However, being a temperate crop, potato is exposed to various environmental stresses, including extended periods of drought and heat. The majority of potato genomics, transcriptomics, and transgenics studies concentrate on the characterization of molecular mechanisms governing cold hardiness of tubers and response and tolerance mechanisms against diseases. Likewise, potato breeding studies focus …

Screening Of Pvlea3 Gene Mrna Expression Levels With Qrt-Pcr In Differentbean Varieties (Phaseolus Vulgaris L.) Subjected To Salt And Drought Stress, İlker Büyük, Sümer Aras

Turkish Journal of Botany

Bean is an edible grain legume species with the greatest selectivity in terms of ecological conditions. Increasing the yield per unit area depends on identification and breeding of species resistant to different ecological conditions. In this regard, we aimed to evaluate mRNA levels of the PvLEA3 gene and lipid peroxidation levels in 7 different bean varieties subjected to NaCl and PEG stresses. Lipid peroxidation analysis (MDA) and qRT-PCR analysis were performed with root and leaf tissues sampled at 3 and 27 h of stress conditions. All results showed that the PvLEA3 gene plays a key role in the defense mechanism …

Evaluation Of Metabolite Extraction Protocols And Determination Ofphysiological Response To Drought Stress Via Reporter Metabolites In Model Plant Brachypodium Distachyon, Özge Tatli, İlkem Emrah Ni̇kerel, Bahar Soğutmaz Özdemi̇r

Turkish Journal of Botany

Metabolomics aims to systematically gather (quantitative) information on metabolites in the cell and is commonly viewed as the "missing link" between genomics, transcriptomics, and physiology. Typical metabolomics platforms consist of two main steps, the quenching and extraction of metabolites from plant material and the (un)targeted quantification of the extracted metabolites. Brachypodium distachyon, a native grass species of the Mediterranean region, is an attractive model plant to study temperate crops. In recent years, despite an increasing interest in genomic and transcriptomic studies, metabolomics studies for B. Distachyon are still in their infancy. Drought, an abiotic stress factor, causes severe loss in …

Ameliorative Role Of Ss-Estradiol Against Lead-Induced Oxidative Stressand Genotoxic Damage In Germinating Wheat Seedlings, Muci̇p Geni̇şel, Hülya Türk, Serkan Erdal, Yavuz Demi̇r, Ebru Genç, İrfan Terzi̇

Turkish Journal of Botany

In the present study, to determine the effects of β-estradiol on the ability of plants to tolerate lead toxicity, β-estradiol (10 μM) and lead (1.75 mM), singly or in combination, were exogenously applied to wheat seeds. Although lead resulted in a marked increase in the activities of antioxidant enzymes, including superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase (but not catalase), as well as an increase in the level of antioxidant compounds such as ascorbic acid and glutathione, this was insufficient to ameliorate the lead-induced oxidative injury or the superoxide anion, hydrogen peroxide, and malondialdehyde levels. However, β-estradiol was …

A Cgh Array Study In Nonsyndromic (Primary) Autism Patients: Deletions On16p13.11, 16p11.2, 1q21.1, 2q21.1q21.2, And 8p23.1, Kudret Esen Gümüşlü, Hakan Savli, Deni̇z Sünnetçi̇, Naci̇ Çi̇ne, Bülent Kara, Seda Eren Keski̇n, Rami̇s Ufuk Akkoyunlu

Turkish Journal of Medical Sciences

Background/aim: To detect specific molecular changes of DNA level in primary autism patients by using whole genome CGH array technology. Materials and methods: A cohort of 35 primary autism patients received clinical genetic testing by using an oligonucleotide-based CGH array platform to test for submicroscopic genomic deletions and duplications. Fluorescent in situ hybridization was performed in seven patients for confirmation of the results. Results: We found 16p13.11 deletion in thirteen patients, 16p11.2 deletion in twelve patients, 1q21.1 deletion in ten patients, 2q21.1q21.2 deletion in eight patients, and 8p23.1 deletion in seven patients. Conclusion: Our study indicates that genes in 16p13.11, …

Relationship Among Mif, Mcp-1, Viral Loads, And Hbs Ag Levelsin Chronic Hepatitis B Patients, Hayati̇ Güneş, Rafet Mete, Murat Aydin, Bi̇rol Topçu, Mustafa Oran, Mustafa Doğan, Oğuzhan Yildirim, İlknur Erdem, Aynur Eren Topkaya, Ahmet Gürel

Turkish Journal of Medical Sciences

Background/aim: To determine whether macrophage migration inhibitory factor (MIF) and monocyte chemoattractant protein-1 (MCP-1) levels in patients with hepatitis B (HB) are different than in normal individuals and whether the HB surface antigen (HBs Ag) level and viral load are correlated with each other and with the two aforementioned parameters. Materials and methods: Sera were obtained from 52 chronic active HB (CAHB) patients and 33 healthy controls, and their MIF and MCP-1 levels were measured. Statistical analyses were performed. A value of P < 0.05 was considered statistically significant. Results: The MIF and MCP-1 values of the control group were increased compared to those of the CAHB group. The MIF and MCP-1 levels were negatively correlated with HBs Ag levels and viral loads. The MIF and MCP-1 levels were positively correlated. The HBs Ag levels and the log10 of the viral loads were positively correlated. Conclusion: We conclude that the negative correlation of MIF and MCP-1 with viral load and HBs Ag levels may be due to T-cell deficiency, antinuclear antibody seropositivity, and/or inhibition of chemokine ligand 2 receptors by viral antigens. More studies with a greater number of subjects are needed to evaluate the potential role of MIF and MCP in CAHB.

Visual And Brainstem Auditory Evoked Potentials In Infants Withsevere Vitamin B12 Deficiency, Ni̇hat Demi̇r, Ahmet Koç, Mahmut Abuhandan, Mustafa Çalik, Akin İşcan

Turkish Journal of Medical Sciences

Background/aim: Vitamin B12 plays an important role in the development of mental, motor, cognitive, and social functions via its role in DNA synthesis and nerve myelination. Its deficiency in infants might cause neuromotor retardation as well as megaloblastic anemia. The objective of this study was to investigate the effects of infantile vitamin B12 deficiency on evoked brain potentials and determine whether improvement could be obtained with vitamin B12 replacement at appropriate dosages. Materials and methods: Thirty patients with vitamin B12 deficiency and 30 age-matched healthy controls were included in the study. Hematological parameters, visual evoked potentials, and brainstem auditory evoked …

Histological And Histochemical Studies On The Structure Of Pancreatic Histological And Histochemical Studies On The Structure Of Pancreatic Ducts Of The Goose (Anser Anser), Turgay Deprem, Serap Koral Taşçi, Seyi̇t Ali̇ Bi̇ngöl, Ebru Karadağ Sari, Şahi̇n Aslan, Serap İlhan

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to determine the histological structure and histochemical properties of the pancreatic ducts in the goose Anser anser. Pancreatic ducts of 6 male and 6 female geese were examined. Crossman’s triple stain and Masson’s trichrome stain were used for histological examination, and periodic acid–Schiff, alcian blue, periodic acid–Schiff/alcian blue, and aldehyde fuchsin/alcian blue stainings were performed for histochemical examination. Dorsal and ventral pancreatic ducts were found that transfer pancreatic secretion into the duodenum of the goose, and these channels were found to have a histologically similar structure. No differences were detected between male and …

Cell-Based Therapy And Rehabilitation With Prosthetic Limbs In A Dog, Jayakrushna Das, Indramani Nath, Padmanav Routray, Rabindra Kumar Das, Sidhartha Sankar Behera

Turkish Journal of Veterinary & Animal Sciences

A 5-year-old male dog was presented with the complaint of a chronic nonhealing wound on both hind legs. The wound occurred during a serious train accident that caused loss of both hind limbs below the hock joint. Since then, the wound did not respond to any standard treatment for up to 4 months. Again it was dressed for 15 days, but it did not show any improvement. With the consent of the owner, cell-based therapy was conducted by collecting bone marrow from the proximal anteromedial aspect of the tibia. After the application of bone marrow derived mesenchymal stem cells (BM-MSCs) …

Molecular Characterization Of The Hspa1a Gene By Single-Strandconformation Polymorphism And Sequence Analysis In Holstein-Friesiancrossbred And Deoni Cattle Raised In India, Ramesha P. Kerekoppa, Akhila Rao, Marappa Basavaraju, Gujjar Ramachandra Geetha, Lakhsminarayan Krishnamurthy, Thota Vl Narasimha Rao, Dayal Nitai Das, Kataktalware Mukund

Turkish Journal of Veterinary & Animal Sciences

A group of proteins called heat shock proteins (HSPs) are synthesized during heat stress. They are expressed at high levels when exposed to stress. Polymorphism in HSPs has been reported to be associated with heat tolerance and reproductive performance in cattle. The present study aimed to investigate the polymorphisms in the coding region of the HSPA1A gene in Holstein-Friesian (HF) crossbreds and the Deoni breed of cattle. Genomic DNA was extracted from 94 animal blood samples and was subjected to polymerase chain reaction-single strand conformation polymorphism analysis, which revealed 14 band patterns in Deoni cattle and 8 band patterns in …

Prevalence Of Toxoplasma Gondii In Sheep Meats Purchased From Retailstores In Central Anatolia, Turkey, Kader Yildiz, Fatma Çi̇ğdem Pi̇şki̇n, Armağan Erdem Ütük, Sami̇ Gökpinar

Turkish Journal of Veterinary & Animal Sciences

Toxoplasmosis is one of the most important foodborne parasitic diseases of humans. In particular, sheep muscles are significant sources of infection in the transmission of toxoplasmosis. Carnivorism is the most important transmission route for human populations. The aim of this study was to detect the prevalence of Toxoplasma gondii tissue cysts in sheep meats in retail stories of Turkey. A total of 250 boneless sheep meat samples were purchased from randomly selected retail stores in different locations of Ankara and Kırıkkale provinces of Turkey. The homogenized meat samples were centrifuged with Percoll dilutions. The tissue cysts were removed by pipette …

Association Of Mx1 Gene Polymorphism With Some Economic Traits In Meishan Pigs, Xue Mei Yin, Ying Liu, Wen Hua Dong, Qiao Hui Zhao, Guo Qiang Zhu, Sheng Long Wu, Wen Bin Bao

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the association of polymorphisms in exon 14 of the Mx1 gene with significant economic traits in Meishan pigs, with the aim of identifying genetic markers of disease resistance. PCR-RFLP analysis of exon 14 of the Mx1 gene was conducted and associations with some cytokine levels (IFN-$\alpha$, IFN-$\gamma$, IL-2, IL-6, IL-10, and IL-12), early body weight (birth weight, 20-day weight, and 40-day weight), and reproductive performance within different parities (total number born, number born alive, number in live weaning litter, birth weight, and weaning weight) were analyzed. The results showed that there were AA, AB, …

Clinical And Radiographic Characterization Of Xenotransplantation Of Rat Bonemarrow-Derived Mesenchymal Stem Cells For Repair Of Radial Defects Of Rabbit, Betania Monteiro, Lukiya Favarato, Pablo Carvalho, Barbara Okano, Maria Antonia Menegatti, Alvaro Oliveira, Bianka Santos, Ricardo Del Carlo

Turkish Journal of Veterinary & Animal Sciences

This study aimed to evaluate the clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived mesenchymal stem cells (MSCs) for the repair of radial bone defects of rabbit. Bone marrow derived MSCs collected from Wistar rats were expanded, differentiated, and labeled in the laboratory. In the fourth passage, the cultures were pelleted in aliquots of 107 cells/50 μL and transplanted in situ into the lesions. Thirty-six rabbits were used in the in vivo study. In all animals, defects were created by removing a bone segment of 1.0 cm in length in the right radius. The rabbits were divided …

A Novel Approach For Determination Of Chicken Sexing At An Early Stage Of Development By Using Loop-Mediated Isothermal Amplification Method, Sung Woo Kim, Jin Seok Choi, Neelesh Sharma, Yeoung Gyu Ko, Yoon Jung Do, Mijeong Byun, Hwan Hoo Seong, Soo Bong Park, Dong Kee Jeong

Turkish Journal of Veterinary & Animal Sciences

The monomorphism of newly hatched chicks is a major hurdle for poultry farmers at the time of sexing. The traditional sexing techniques are inaccurate and time-consuming. Therefore, the present study aimed to develop a loop-mediated isothermal amplification (LAMP)-based sex determination method to obtain a higher separation accuracy rate as compared to conventional methods. LAMP is a simple, quick, and relatively inexpensive diagnostic tool. Genomic DNA was extracted from the blood samples of Leghorn and Korean native chicks for LAMP. Two sets of primer were designed, consisting of four isothermal amplification primers in each, to amplify the female sex-specific sequence (HUR0417) …

Prevalence Of Blaz Gene And Other Virulence Genes In Penicillin-Resistant Staphylococcus Aureus Isolated From Bovine Mastitis Cases In Gansu, China, Feng Yang, Qi Wang, Xurong Wang, Ling Wang, Min Xiao, Xinpu Li, Jinyin Luo, Shidong Zhang, Hongsheng Li

Turkish Journal of Veterinary & Animal Sciences

Staphylococcus aureus is a major etiological agent of bovine mastitis worldwide. In this study, 37 strains of S. Aureus resistant to penicillin were isolated from bovine mastitis cases in Gansu Province for investigating blaZ and virulence-related genes, including tst, eta, etb, lukPV, lukED, lukM, hla, hlb, hld, and edin. Antibiotic resistance was based on disk diffusion method and blaZ and virulence-associated genes were studied by polymerase chain reaction. Penicillin resistance gene blaZ was detected in 35/37 (94.6%) of penicillin-resistant S. Aureus isolates. tst, lukPV, lukED, hla, hlb, and hld were observed in 5.4%, 2.7%, 89.2%, 70.3%, 73.0%, and 70.3% of …

Significance Of Homocysteine And Cardiac Markers In Cattle With Hypocalcemia, Yildiray Başbuğan, Nazmi̇ Yüksek, Nuri̇ Altuğ

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the levels of homocysteine and cardiac markers in cows with hypocalcemia. This research was conducted with a total of 20 cows, including 10 with hypocalcemia and 10 healthy controls. Hypocalcemia was diagnosed by determining low blood calcium (Ca) levels, and a standard hypocalcemia treatment was initiated in those cows with the disease. Blood samples were taken before the treatment and again 12 h after the treatment, and there was no significant difference in the homocysteine levels between the groups. Ca, ionized calcium (Ica), magnesium (Mg), and phosphorus (P) levels were lower, while cardiac troponin I …

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

2015-2016 Graduate Catalog, Morehead State University

Morehead State Catalog Archive

2015-2016 graduate catalog for Morehead State University.

2015-2016 Undergraduate Catalog, Morehead State University

Morehead State Catalog Archive

2015-2016 undergraduate catalog for Morehead State University.

Primer Efficacy In The Dna Barcoding Of Spiders, Rhennetta Jo Bork

Honors Program Theses

DNA barcoding is the process of amplifying a 650 base pair segment of the sequence of the mitochondrial gene cytochrome c oxidase (COI), and amplifying this gene with a polymerase chain reaction (PCR). It is used to help identify and distinguish animal species and also to help determine genetic differences in species. DNA barcoding can be especially useful when working with spiders since they tend to be very small and hard to distinguish. However, achieving a DNA barcode can be difficult and thus techniques to improve the method of DNA barcoding can be helpful. This research looked into the different …

Stare Decisis In The Second-Best World, Randy J. Kozel

Journal Articles

If judges disagree about the proper interpretation of the law, can they find common ground in the treatment of precedent? The doctrine of stare decisis weighs the value of legal continuity against the value of legal accuracy. But that analysis is complicated by pervasive disputes over the implications of legal error. Likewise, the scope of a precedent's impact in future cases depends on underlying assumptions about the ends and means of legal interpretation. When those assumptions vary among judges, so too will applications of stare decisis. For stare decisis to promote the continuity and impersonality of law, it requires an …

The Fair Market Value Of Public Resources, Bruce R. Huber

Journal Articles

This Article explores the problem of public resource sales with particular reference to natural resources managed by the federal government. Lands owned by the United States hold trillions of dollars' worth of natural resources. Federal agencies earn billions in annual revenue from resource sales, yet critics assert that billions more could be reaped if resources were sold for a fair price. Although federal law has increasingly required that agencies price resources at fair market value, this requirement is surprisingly difficult to interpret and even more dfficult to implement and enforce. This Article analyzes the various forces that bear on public …

The Hughes Court Docket Books: The Late Terms, 1937–1940, Barry Cushman

Journal Articles

For many years, the docket books kept by a number of the justices of the Hughes Court have been held by the Office of the Curator of the Supreme Court. Yet the existence of these docket books was not widely known, and access to them was highly restricted. Recently, however, the Court adopted new guidelines designed to increase access to the docket books for researchers. This article offers the first-ever examination of the available docket book entries relevant to what scholars commonly regard as the major decisions of rendered during the late years of the Hughes Court, from the 1937 …

De Novo Assembly Of The Chimpanzee Transcriptome From Nextgen Mrna Sequences, Mnirnal D. Maudhoo, Jacob D. Madison, Robert B. Norgren

Journal Articles: Genetics, Cell Biology & Anatomy

BACKGROUND: Common chimpanzees (Pan troglodytes) and bonobos (Pan paniscus) are the species most closely related to humans. For this reason, it is especially important to have complete and accurate chimpanzee nucleotide and protein sequences to understand how humans evolved their unique capabilities. We provide transcriptome data from four untransformed cell types derived from the reference Pan troglodytes, "Clint", to better annotate the chimpanzee genome and provide empirical validation for proposed gene models of this important species.

FINDINGS: RNA was extracted from primary cells cultured from four tissues: skin, adipose stroma, vascular smooth muscle and skeletal muscle. These four RNA samples …

Lead Exposure Of Obligate Avian Scavengers In Eastern North America, Shannon Behmke

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lead is a toxic element within the body of living organisms. Lead poisoning cases still occur in both humans and wildlife on a regular basis in the United States. Although the harmful effects of lead within the body are well known, such as decreased IQ in children, increased renal dysfunction, hypertension, anemia, and other morbid and mortal consequences in humans and wildlife, lead continues to be used in anthropogenic activities today. Through the use of lead in ammunition and fishing sinkers, and its presence in coal-fired power plant and other industrial emissions, lead is dispersed throughout modern human and natural …

2015 Annual Research Report: Greater Sage-Grouse Habitat Use Patterns And Vital Rates In The Morgan-Summit Sage-Grouse Management Area: Conservation Implications For Managers, B. Flack, Terry A. Messmer

Wildland Resources Faculty Publications

No abstract provided.

Freedom And Fetters: Nuptial Law In Burney’S The Wanderer, Melissa J. Ganz

English Faculty Research and Publications

No abstract provided.