Digital Commons Network^™

Role Of The Intestinal Microbiota In Gut Barrier Dysfunction Following Burn Injury, Zachary Earley

Master's Theses

Burn injury represents a major medical problem with half a million cases requiring medical attention and 4,000 deaths reported annually. Sepsis and multiple organ failure remain the leading causes of death following injury, and may be brought on by bacterial infections or toxins. The gastrointestinal tract contains approx. 100 trillion microbes; therefore, the indigenous commensal microbiota may play a role in leading to these complications or infections in burn patients. The overall objective of this project is to identify a potential mechanism whereby changes in gut bacteria may lead to intestinal inflammation or bacterial translocation--key factors which may lead to …

Life Histories, Diets, And Secondary Production Of Odonata Along A Temperature Gradient On The Copper River Delta, Alaska, Nicole Emilie Furlan

Master's Theses

Dragonflies (Odonata: Epiprocta) and damselflies (Odonata: Zygoptera) are a conspicuous aspect of the biota in ponds on southcentral Alaska's Copper River Delta (CRD). Odonate densities, secondary production, and diets were assessed in sixteen ponds classified by delta region (east vs. west) and landscape type (outwash plain (OP) vs uplifted marsh (UM)).

Enallagma boreale (Coenagrionidae) comprised 48.5% of collected odonates. Leucorrhinia hudsonica (Libellulidae) and Aeshna juncea (Aeshnidae) comprised 36.6% and 10.4% of collected odonates, respectively. L. hudsonica densities and secondary production were significantly higher (p<0.001) in west UM ponds than in other pond types. Ostracods (Ostracoda) and water boatmen (Corixidae) dominated west OP A. juncea diets. Midge larvae (Chironomidae) dominated A. juncea diets in remaining pond types, occurring in 68% of foreguts. 27% of A. juncea foreguts demonstrated intraguild predation, and 6% of foreguts demonstrated cannibalism. Foreguts containing threespine stickleback (Gasterosteus aculeatus) revealed A. juncea's apex predator role in CRD ponds.

Identifying Oncogenic Drivers In Nsclc Cells Harboring Egfr Kinase Domain Mutation With Resistance To Egfr Tki And Mesenchymal Phenotype, Yandi Gao

Master's Theses

EGFR kinase domain mutant NSCLC cells are exquisitely dependent on mutant EGFR for cell survival and proliferation. Patients with mutant EGFR respond well to the EGFR inhibitors. However, acquired drug resistance greatly limits the efficacy of the treatment. About 15% of the resistant tumors present an evidence of epithelial to mesenchymal transition (EMT). We hypothesize that the induction of mesenchymal promotes aberrant upregulation of other oncogenic drivers to replace the oncogenic mutant EGFR.

We observed overexpression of CXCR7 in NSCLC models of acquired resistance to EGFR TKI with mesenchymal phenotype. Additionally, our studies demonstrate that ectopically overexpressing CXCR7 in EGFR …

Reconsidering The Model Of Trim5Α Assembly: The Role Of The Linker2 (L2) Region In Trim5Α Assembly, Laura Johnsen

Master's Theses

The TRIM5α protein from rhesus macaques (TRIM5αrh) exhibits a remarkable ability to potently inhibit infection by Human Immunodeficiency Virus Type-1 (HIV-1). Extensive studies have shown that TRIM5α is capable of self-associating at many levels, eventually leading to the formation of a hexameric assembly that can superimpose on the hexameric lattice of the HIV-1 capsid. The mechanism underlying the self-association of TRIM5α and the molecular determinants of self-association remain to be completely understood. In this study, we show that the Linker 2 (L2) region of TRIM5rh is important for dimerization and higher order self-association, both of which are independent processes. Additionally, …

Characterizing The Mechanisms By Which Community Associated Methicillin-Resistant Staphylococcus Aureus Influences Keratinocyte Innate Immune Responses During Recurrent Infection, Ashley Lynn Larm

Master's Theses

Community associated–methicillin resistant Staphylococcus aureus (CA–MRSA) infection has become a major health concern. In human epidermal keratinocytes, S. aureus is mainly recognized through toll–like receptor 2 (TLR2) and its co–receptor, CD14. We hypothesize that CA–MRSA isolates cause recurrent infections by interrupting TLR2–mediated inflammation in keratinocytes. Recurrent CA–MRSA bacterial culture supernatant exposure to keratinocytes in vitro resulted in significant decreases in pro and anti–inflammatory cytokine and HMGB1 secretion from keratinocytes as assessed by ELISAs. Recurrent CA–MRSA live infection did not result in significant changes in cytokine or HMGB1 secretion, surface receptor expression, or NFκB activation post infection as assessed by ELISA …

History Of Freshwater Invasions In Illinois: Learning From The Past To Inform The Future, Abigail Jacobs

Master's Theses

Rates of introduction and spread of non-native species continue to increase worldwide, with freshwater ecosystems highly impacted. Many non-native species crossed between the Great Lakes and Mississippi watersheds by traveling through waterways in Illinois. I assembled a comprehensive database of occurrences of aquatic non-native species (n=99) in Illinois inland waters. The arrival of non-native species accelerated since 1873. The Great Lakes Basin was the main source of established species into Illinois. From 1990-2012 the dominant vector was unintentional release of established species non-native to North America. Sixty of the 99 introduced species established. Eighteen established species had high or very …

Denitrification In Urban Coastal Environments: A Functional Gene Study, Samantha Denise Lindemann

Master's Theses

Eutrophication is a major anthropogenic stressor on aquatic ecosystems worldwide. Jamaica Bay is located in the southeastern portion of New York City, NY (NYC). Like many other estuaries, Jamaica Bay is impacted by anthropogenic N inputs from various sources. Despite eutrophic conditions, improvements in water quality over the last few decades have prompted government agencies to promote oyster restoration to help reduce anthropogenic N in NYC waters. The effect of eastern oysters on sediment bacterial communities responsible for denitrification in Jamaica Bay was previously unknown. Those samples exposed to oysters were predicted to have high denitrification gene abundances, and thus …

Effects Of Instruction And Parent-Child Conversation On Children's Stem Learning And Transfer, Maria Marcus

Master's Theses

This study examined the effects of direct instruction and parent-child conversation on children's STEM learning, transfer abilities, and remembering. A total of forty mothers and their 5- to 6-year-old children (M = 5.87) participated in this study. Mother-child dyads were randomly assigned to one of two conditions that differed in the amount of engineering information they received prior to engaging in a building activity in a museum exhibit. The provision of engineering information fostered dyads building activities and their long-term recall of the museum visit. Implications for museum research and practice are discussed.

Characterization Of The Female Urinary Microbiota And Their Association With The Female Bladder Uroepithelium, Evann Elizabeth Hilt

Master's Theses

The current clinical dogma assumes that urine is sterile in the absence of clinically relevant infection. However, recent evidence has demonstrated the existence of a female urinary microbiota in women with and without lower urinary tract symptoms. With the knowledge that the lower urinary tract possesses its own unique microbiota, I hypothesize that certain bacterial species of the female microbiota may be the cause or play a role in lower urinary tract syndromes such as overactive bladder syndrome (OAB). About 40-50% of OAB patients do not respond to conventional anti-muscarinic and beta-3 adrenergic agonist drug treatment. One possible explanation for …

An Investigation Of The Role Of Vibrio Vulnificus Rbda And Vibrio Parahaemolyticus Sypa In Biofilm Formation, Cecilia Thompson

Master's Theses

Vibrio vulnificus and Vibrio parahaemolyticus are both bacteria that cause human infection. V. vulnificus has a polysaccharide locus, rbd, responsible for bacterial aggregation, a form of biofilm. This locus is conserved in V. parahaemolyticus and in the non-pathogen V. fischeri.

In V. fischeri, the polysaccharide locus, syp, has been extensively characterized and shown to be important for biofilm formation. In V. fischeri, the first gene, sypA, is critical for biofilm formation. V. fischeri biofilm-proficient strains form wrinkled colonies. In contrast, sypA mutants form smooth colonies, indicating a lack of biofilm formation.

To understand the function of RbdA and SypAVP, proteins …

Posttraumatic Stress, Family Functioning, And Adjustment In Urban African American Youth Exposed To Violence: A Moderated Mediation Model, Kyle Deane

Master's Theses

Exposure to community violence is a pressing public health issue that disproportionately impacts poor, urban, and ethnic minority youth. It has been associated with a multitude of negative externalizing and internalizing symptoms, most frequently with posttraumatic stress. This study investigates the role that posttraumatic stress has in mediating the relation between exposure to community violence and other adjustment difficulties. Moreover, because not all adolescents experience these difficulties in the face of significant violence exposure, the study examines the moderating role of family cohesion and support in buffering the effect of violence and posttraumatic stress on later adjustment. A sample of …

Sexual Behavior Problems In Child Welfare: Predictors Of Reliable Change, Anne Kathleen Fuller

Master's Theses

This study examined predictors of changes in children's sexual behaviors across two time points within a sample of youth in the child welfare system. Hypothesized predictors of increases or decreases in children's sexual behaviors included child attributes, positive parenting, exposure to sexuality and violence, maltreatment history and child welfare placement history, and treatment variables. Participants included 145 children with reported sexual behavior problems and their primary caregivers and mental healthcare providers. Children's sexual behaviors were classified as improved, worsened, or unchanged. Optimal Data Analysis (ODA) and multivariate classification tree analysis (CTA) via ODA were used to identify predictors of children's …

A Study Of Functional Recovery And Axonal Plasticity Following Tbi And Anti-Nogo-A Immunotherapy, Jennifer Crystal Biesiadecki

Master's Theses

Traumatic brain injury (TBI) is a leading cause of morbidity and mortality globally and often results in long term disability. Unfortunately, the success of rehabilitation techniques as therapy for TBI is limited, which may be due to the growth restrictive environment of the adult central nervous system (CNS). This environment is thought to result from glial scars, lack of neurotrophic factors, and the presence of an array of growth-inhibitory molecules. One such growth-inhibitory molecule is the protein Nogo-A. Our laboratory has shown that neutralization of Nogo-A with anti-Nogo-A immunotherapy after ischemic stroke results in improvement in functional recovery in the …

Learning To Tell Coherent Personal Narratives: Linkages To Mother-Child Reminiscing Over Time, Philip C. Hoffman

Master's Theses

Longitudinal and concurrent predictors of children's narrative coherence are identified and used to model pathways to coherence. Narrative coherence in children's independent narratives was measured at 72-months using a multidimensional (context, chronology, and theme) coding system. Fifty-three potential predictors of children's narrative coherence were considered, including children's vocabulary scores, metamemory knowledge, and measures derived from observations of mothers' and children's talk during reminiscing conversations recorded when the children were 54 and 72 months old. Optimal Data Analysis was used to generate three classification tree models to identify variables associated with whether children were low or high on three dimensions of …

Examining The Moderating Role Of Specific Coping Strategies On The Relationship Between Body Image And Eating Disorders In College-Age Women, Alexandra Calvert Kirsch

Master's Theses

A sample of college age women assessed at three time points (Time 1: Baseline, assessed before college, Time 2: End of first semester, Time 3: End of first year of college) completed measures of disordered eating, coping, and body image. Results indicated that neither adaptive (problem-focused coping or social support seeking) nor maladaptive coping styles (active emotional coping or avoidant coping) as measured at Time 1 or Time 2 moderated the significant predictive relationship between body dissatisfaction at Time 1 and disordered eating attitudes at Time 3, when adjusting for disordered eating attitudes and BMI at Time 1. However, significant …

Conference Program [2014], Gurc

Georgia Undergraduate Research Conference (Archived)

N/A

Numerical Study Of Lipid Translocation Driven By Nanoporation Due To Multiple High-Intensity, Ultrashort Electrical Pulses, Viswanadham Sridhara, Ravindra P. Joshi

Electrical & Computer Engineering Faculty Publications

The dynamical translocation of lipids from one leaflet to another due to membrane permeabilization driven by nanosecond, high-intensity (>100 kV/cm) electrical pulses has been probed. Our simulations show that lipid molecules can translocate by diffusion through water-filled nanopores which form following high voltage application. Our focus is on multiple pulsing, and such simulations are relevant to gauge the time duration over which nanopores might remain open, and facilitate continued lipid translocations and membrane transport. Our results are indicative of a N^1/2 scaling with pulse number for the pore radius. These results bode well for the use of pulse …

Plasma Processes And Polymers Special Issue On: Plasma And Cancer, Mounir Laroussi, Michael Keidar

Electrical & Computer Engineering Faculty Publications

During the last two decades, research efforts on the application of low temperature plasmas in biology and medicine have positioned nonequilibrium lowtemperature plasmas as a technology that has the potential of revolutionizing healthcare.[1,2] Low temperature plasmas can be applied in direct contact with living tissues to inactivate bacteria,[3] to disinfect wounds and accelerate wound healing,[4] and to induce damage in some cancer cells.[5–11]

Preparing Young Writers For Invoking And Addressing Today’S Interactive Digital Audiences, Ewa Mcgrail, J. Patrick Mcgrail

Research, Publications & Creative Work

Twenty-first century technologies, in particular the Internet and Web 2.0 applications, have transformed the practice of writing and exposed it to interactivity. One interactive method that has received a lot of critical attention is blogging. The authors sought to understand more fully whom young bloggers both invoked in their blogging (their idealized, intentional audience) and whom they addressed (whom they actually blogged to, following interactive posts). They studied the complete, yearlong blog histories of fifteen fifth-graders, with an eye toward understanding how these students constructed audiences and modified them, according to feedback they received from teachers as well as peers …

Purpose And Design In Organisms And Artifacts: The Search For A Unified Philosophical Theory Of Function, Mark L. Bourgeois

Dissertations

This work examines the concept of function in both biological organisms and designed artifacts. Function is routinely attributed within both kinds of systems, yet it is unclear whether it can mean the same thing within each, and indeed whether function attribution within natural systems is legitimate at all. Function is typically understood to have teleological content; yet in true teleological causation, the end is somehow the cause of its own means. In artifacts the consciously envisioned design of an artifact is taken to be the cause of its parts and their roles. Yet in naturalistic science there is no prior …

Understanding The Impact Of Adolescent Attachment On Academic Success, Cristina Cortesi

Dissertations

In recent years, studies are citing an increase in adolescent mental health diagnoses and symptomotology related to AD/HD, Bipolar Disorder, Depression, Anxiety, and Conduct Disorders. While the associated behaviors may be the result of several variables, recent neurobiological studies combined with Attachment Theory have pointed to a possible link to attachment issues. Because of the developmental stage of adolescents, these behaviors have been observed in both home and school environments. Schools, in general in the United States, have been experiencing significant struggles in terms of lack of adequate educational success which prompted the implementation of legislation commonly known as No …

Just Water: A Feminist Catholic Response To The Commodification Of Water, Rachel Noelle Hart Winter

Dissertations

Any Catholic ecological ethic today that does not focus sustained attention to our worldwide water crisis is inadequate, for it fails to engage one of today's core social justice violations and neglects to offer any moral guidance for one of the human family's most pressing challenges. A responsible Catholic approach to water justice that addresses the problems stemming from a commodified view of water must be informed by ecofeminist concerns and by the Catholic social justice tradition of moral reasoning. As populations grow and water sources run dry, access to water has become a pressing ethical issue. Today, nearly one …

Teacher Impact On Supporting The Parent-Teacher Partnership During The Middle School Years, Karen R. Love

Dissertations

The purpose of this study was to investigate the perceptions and experiences of middle school teachers in supporting parents as they move from traditional forms of parental involvement and towards engagement to support their children as they move into the middle school years of education. The design of this study was qualitative using in-depth interviews of middle school teachers. Bronfenbrenner's bioecological system theory of human development was the theoretical framework for this study to examine the factors influencing the child's development during the middle school years. Participants demonstrated that their perceptions and experiences did impact how they viewed parental involvement …

Life Course Narratives From U.S. Expatriates: Continuity Work, Victoria L. Russo

Dissertations

This qualitative, exploratory research examined the American life course through narrative accounts of twenty, former expatriates. All participants had lived and worked in another country for a minimum of at least one year before returning to the US. In-depth, face-to-face interviews were conducted between April 2011 and June 2012. Interviews were unstructured and lasted between 1-2 hours.

Participants in this study were well-educated, middle class professionals with highly desirable skills. Yet, despite the flexibility that privileged status bestowed, participants maintained normative life course patterns. Families were instrumental in monitoring the life course and distributed sanctions and benefits to family members …

The Effects Of Racial Identity On African American Youths' Psychosocial Adjustment: A Conceptualization Of The Literature And Meta-Analytic Review, Corinn Elmore

Dissertations

There is a general assumption of the positive effect of racial identity on the psychosocial adjustment of African-American youth. Despite this assertion, research findings for racial identity are unclear. The disparate measures of racial identity were organized into a cohesive framework with eight categories. Using this conceptual framework, a meta-analytic review was conducted on the effects the components of racial identity on African-American adolescents' psychosocial adjustment. There were 58 independent samples from 34 published journal articles and 14 unpublished papers (dissertations) including a total of 14,209 youth included in the study. Results of study highlight the importance of racial pride …

Journey To The Neighborhood: An Analysis Of Fred Rogers And His Lessons For Educational Leaders, Jeana Lietz

Dissertations

Millions of children worldwide grew up watching Fred Rogers, aka “ Rogers,” as their childhood neighborhood friend on the television show Mister Rogers’ Neighborhood . How was a man able to produce one of the most successful, longest running children’s television programs and stay true to his moral principles? This study examines the life and work of Fred Rogers, and identifies the leadership attributes of Fred Rogers and the implications for educational leaders, through the lens of the five sources of authority described in Moral Leadership: Getting to the Heart of School Improvement by Thomas Sergiovanni (1992).

The central research …

Investigating The Biochemical And Catalytic Properties Of Nitrile Hydratases, Salette Martinez

Dissertations

Chemical and pharmaceutical industries make extensive use of amide compounds for the manufacture of commodity chemicals (e.g., acrylamide) and drug intermediates (e.g., nicotinamide). Production of amide compounds is typically achieved by the hydration of nitrile compounds under acidic or basic conditions, high temperatures, and copper catalysts. However, the use of such chemical methods leads to the generation of unwanted by-products and toxic wastes, in addition to low product yields and high production costs. An alternative route for amide production is the use of a natural catalyst, for example nitrile hydratases (NHase, E.C. 4.2.1.84). NHase is a metalloenzyme that efficiently converts …

Sorority Women & Eating Pathology: Communities Of Unhealthy Body Standards?, Ashley Marie Rolnik

Dissertations

The present study examines disordered eating, sorority social norms regarding the body and thinness, personal values regarding the body and thinness, body dissatisfaction, thin ideal internalization, fat talk, negative affect, and positive affect among sorority and non-sorority women. The aims of this study are to discern the underlying factor structures of the sorority/group social norms questionnaires and the personal values regarding the body and thinness questionnaire, investigate disordered eating among sorority and non-sorority women over time, and further examine the impact of social norms on sorority women's body and eating attitudes and behaviors. The results of this research illustrated three …

Mutations In Phospholamban Alter The Structure And Function Of The Calcium Atpase Regulatory Complex, Neha Abrol

Dissertations

Phospholamban (PLB) is an integral membrane protein that plays an important role in regulation of cardiac calcium handling and contractility. PLB exists as a homopentamer in the membrane, which upon deoligomerization into active monomers reversibly inhibits sarco/endoplasmic reticulum calcium ATPase (SERCA). Mutations in PLB that change the PLB monomer-pentamer equilibrium result in dysregulation of SERCA. To determine the structural and regulatory role of the C-terminal residues of PLB in the membranes of living cells, we fused fluorescent protein tags to PLB and SERCA. We then studied the effect of C-terminal alanine substitutions and truncation mutations on PLB oligomerization and SERCA …

The Role Of Spirituality/Religion As A Coping Mechanism During Treatment For Disordered Eating, David Franczyk

Dissertations

The purpose of this study was to investigate the role of spirituality/religion used as a coping mechanism during treatment for disordered eating. Given the mixed outcome results of current therapeutic and pharmacological treatment methods for disordered eating, it is important to investigate other factors which may influence the treatment process. This study evaluated the role of spirituality/religion used as a coping mechanism among 61 patients who were admitted into an eating disorder treatment program and then discharged over a period of 15 months. In this quantitative study, the Brief RCOPE measure was self-administered at admission to determine the levels of …