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Full-Text Articles in Entire DC Network
The Modern Gender Gap In Partisanship And Ideology: A Cross-National Analysis, Tiffany Quick
The Modern Gender Gap In Partisanship And Ideology: A Cross-National Analysis, Tiffany Quick
Electronic Theses and Dissertations
This thesis updates and expands upon the developmental theory of the gender gap in party and ideological identification originally posited by Norris and Inglehart (2000) to explain why women in advanced industrial nations are more likely to hold more leftist ideological identification than men. A comparative cross-national analysis using data from the World Values Survey (2004-2008) extends Norris and Inglehart's study, with an examination of the gender gap in advanced industrial, post-communist and developing nations. To further explore the nature of the gender gap in the United States, data from the American National Election Study (Cumulative File and 2012 cross-section) …
Movements, Fishery Interactions, And Unusual Mortalities Of Bottlenose Dolphins, Steven Shippee
Movements, Fishery Interactions, And Unusual Mortalities Of Bottlenose Dolphins, Steven Shippee
Electronic Theses and Dissertations
Bottlenose dolphins inhabiting coastlines and estuaries in Florida have been impacted in the past decade by development, algal blooms, catastrophic pollution, and fishery interactions (FI). Dolphins react to disturbance and environmental stressors by modifying their movements and habitat use, which may put them in jeopardy of conflict with humans. FI plays an increasing role in contributing to dolphin mortalities. I investigated dolphin movements, habitat use, residency patterns, and frequency of FI with sport fishing. Tagging studies with short-term data tags and bolt on radio-transmitters were done in several locations in Florida and the east coast providing fine-scale measurements of swimming, …
The Role Of Invitational Theory On Minority Student Enrollment In Advanced Placement Courses, Jason Mcdonald
The Role Of Invitational Theory On Minority Student Enrollment In Advanced Placement Courses, Jason Mcdonald
Electronic Theses and Dissertations
The number of students enrolling in Advanced Placement (AP) classes has been increasing in Florida and across the nation over the last decade. However, this trend is not happening for traditionally underserved groups of students such as African Americans, Hispanics, and students from low socioeconomic backgrounds. These minority groups are underrepresented in AP classes, while Asian and White students are overrepresented. This trend is alarming because there are qualified minority students who have a great chance of being successful in AP classes according to AP Potential data. For some reason though, these qualified minority and low income students are nevertheless …
Photo-Induced Protonation Of Polyaniline Composites And Mechanistic Study Of The Degradation Of Polychlorinated Biphenyls With Zero-Valent Magnesium, Candace Kirkland
Photo-Induced Protonation Of Polyaniline Composites And Mechanistic Study Of The Degradation Of Polychlorinated Biphenyls With Zero-Valent Magnesium, Candace Kirkland
Electronic Theses and Dissertations
As technology advances, a need for non-metal, conductive materials has arisen for several types of applications. Lithographic techniques are helpful to develop some of these applications. Such techniques require materials that are insulating and become conductive after irradiated. Composites of polyaniline in its emeraldine base form (PANI-EB) doped with photo-acid generators (PAG) become conductive upon photo-irradiation. This increase in conductivity is due to the protonation of PANI-EB. Such materials may be utilized to fabricate conducting patterns by photo-irradiation; however, the conductivity obtained by direct irradiation of PANI-EB/PAG composites is normally quite low (<10-3 S/cm) due to aggregation of highly loaded PAG. In this work, poly(ethylene glycol) (PEG), a proton transfer polymer, was added to PANI-EB/PAG. Results showed the addition of low molecular weight (MW) (550) PEG significantly enhanced the photo-induced conductivity to a level comparable to that of PANI-salt synthesized by oxidizing aniline in the presence of an acid. High MW (8000) PEG is less effective than PEG 550, and composites of PANI-EB and N-PEG-PANI showed conductivity as high as 102 S/cm after treatment with HCl vapor. The photo-induced conductivity of the N-PEG-PANI/PANI-EB/PAG composite reached 10-2-10-1 S/cm. Polychlorinated biphenyls (PCBs) are a class of chemicals with 209 different congeners, some of which are known carcinogens, and are persistent organic pollutants in the environment. After its synthesis, it was seen as a phenomenal additive in a multitude of different applications leading to the wide spread use of PCBs and a need for a safe, effective, and inexpensive remediation technique. While it is known that magnesium can degrade PCBs, the mechanism of this reaction was not well-understood. In order for magnesium to be broadly used as a remediation tool, it is necessary to fully understand how the reaction is taking place and if the PCBs are able to be fully dechlorinated into biphenyl. This research focuses on the hydrodechlorination of PCBs with zero-valent magnesium in acidified ethanol. The degradation pathways of 2, 2', 3, 5, 5', 6- hexachlorobiphenyl were investigated to determine the identity of the daughter PCBs produced, how and if they continue to be dechlorinated into biphenyl. The proton source for this hydrodehalogenation reaction was also studied using both deuterated solvent and acid to give more detail to the mechanism of this reaction.
Mothers' Temperament And Personality: Their Roles In Parenting Behaviors, Parent Locus Of Control, And The Outcomes Of Young Children, Jayme Puff
Electronic Theses and Dissertations
Many researchers have used the terms 'temperament' and 'personality' interchangeably when describing parents' behavioral styles. Although individual relationships among parents' temperament and personality, parenting behaviors, other parent characteristics, and young children's outcomes have been documented in the literature, parents' temperament and personality have not been examined collectively in conjunction with parenting and child outcome variables. As part of this study, 214 culturally diverse mothers with young children who ranged in age from 2- to 6-years rated their own temperament and personality, their parenting characteristics, and their young child's functioning (i.e., temperament and emotional and behavioral functioning). When examining mothers' temperament …
Physiological Constraints On Warm-Water Habitat Site Selection And Utilization By The Florida Manatee (Trichechus Manatus Latirostris) In East Central Florida, Ann Spellman
Electronic Theses and Dissertations
Living at the northern limits of its geographic range, the Florida manatee is particularly susceptible to cold stress-related mortality during the winter months, with most deaths occurring in the lower two-thirds of the state. Contributing to this cold stress susceptibility is the manatee's limited physiological and behavioral responses available when thermally stressed. While capable of migrating south in response to falling water temperatures, manatees must still find warm water when ambient river temperature drops below 20°C for more than a few days. This is in part due to the species low metabolic rate, limited capacity for thermogenesis, and limited ability …
Role Of Cardiac Catecholamines In Embryos And Adults Under Stress, Candice Baker
Role Of Cardiac Catecholamines In Embryos And Adults Under Stress, Candice Baker
Electronic Theses and Dissertations
Cardiovascular disease is responsible for the loss of one life every 38 seconds and accounts for 26.6 percent of all infants that die of congenital birth defects. Adrenergic hormones are critically important regulators of cardiovascular physiology in embryos and adults. They are key mediators of stress responses and have profound stimulatory effects on cardiovascular function, and dysregulation of adrenergic function has been associated with many adverse cardiac conditions, including congenital malformations, arrhythmias, ischemic heart disease, heart failure, and sudden cardiac death. Despite intensive study, the specific roles these hormones play in the developing heart is not well-understood. Further, there is …
Structural And Functional Studies Of Glycine Riboswitches And Development Of Fab Chaperone Assisted Rna Crystallography, Eileen Sherman
Structural And Functional Studies Of Glycine Riboswitches And Development Of Fab Chaperone Assisted Rna Crystallography, Eileen Sherman
Electronic Theses and Dissertations
The glycine riboswitch is a structured RNA found upstream of genes in mRNA transcripts in many bacteria, functioning as a biofeedback gene regulator. Upon binding glycine, a complete RNA transcript including gene sequences is transcribed, effectively turning on gene expression. In an effort to understand the intricacies of its functioning, many mutants of the riboswitch were made and characterized during Ph. D. work, resulting in discovery of a P0 duplex/kink-turn motif involving a few nucleotides upstream of the established glycine riboswitch sequence which changed its ligand binding characteristics (Chapter 1). Previously, the two aptamers of the riboswitch were thought to …
Eurocentrism, Modernity And Chinese Sociality : An Ethnographic Study Of Everyday Socio-Cultural Life In New-Millennium China, Tung Yi Kho
Lingnan Theses
This dissertation seeks to contribute to our understanding of Eurocentrism, Modernity,and their impacts on traditional Chinese cultural formations. It is based on ethnographic research conducted in Shenzhen, South China and Qinghai, West China, and explores the impacts of Chinese modernization on everyday forms of sociality.
I conceive of Eurocentrism foremost as an Ontology, a mode-of-being grounded in a cosmology about the nature of reality and human being-and-becoming in the world. I argue that because Eurocentrism is an ontology predicated on materialism and individualism - “matter” being the basis of reality and the “individual”, the fundamental unit of society, respectively - …
Catholic Healing Masses: Intersections Of Health And Healing In Yucatan, Suzanne Draper
Catholic Healing Masses: Intersections Of Health And Healing In Yucatan, Suzanne Draper
Electronic Theses and Dissertations
The conception of illness and healing in contemporary Mexican Catholic discourse highlights both particular and ubiquitous instances of a health experience perceived locally and widespread. Catholic healing masses are utilized as supplemental methods of individual health restoration coupled with Western medicinal techniques in Catholic dramas. Aside from the spiritual and religious significance of this practice, the use of healing masses as an additional means to achieving an optimal health status implies that something is lacking in current biomedical models. The purpose of my research is to explore the humanistic terms under which healing masses operate and translate these terms into …
Ar Physics: Transforming Physics Diagrammatic Representations On Paper Into Interactive Simulations., Yao Zhou
Ar Physics: Transforming Physics Diagrammatic Representations On Paper Into Interactive Simulations., Yao Zhou
Electronic Theses and Dissertations
A problem representation is a cognitive structure created by the solver in correspondence to the problem. Sketching representative diagrams in the domain of physics encourages a problem solving strategy that starts from 'envisionment' by which one internally simulates the physical events and predicts outcomes. Research studies also show that sketching representative diagrams improves learner's performance in solving physics problems. The pedagogic benefits of sketching representations on paper make this traditional learning strategy remain pivotal and worthwhile to be preserved and integrated into the current digital learning landscape. In this paper, I describe AR Physics, an Augmented Reality based application that …
A Thomistic Critique Of The Ethics Of Alasdair Macintyre, Marcus Otte
A Thomistic Critique Of The Ethics Of Alasdair Macintyre, Marcus Otte
Electronic Theses and Dissertations
Alasdair MacIntyre argues in favor of a historicist Thomism in ethics and political philosophy. In his theory, sociological categories take up much of the space traditionally occupied by metaphysics. This peculiar feature of MacIntyre's Thomism, and its merits and demerits, is already a subject that has been taken up by many critics. In this thesis, these criticisms are supplemented and unified by identifying what is perhaps the most fundamental difficulty with MacIntyre's ethics: his version of Thomism is problematic because it treats epistemology as first philosophy. This misstep compromises MacIntyre's ability to provide a defense of moral objectivity, while also …
Evaluation Of A Digitally-Automated Alcohol Curriculum Designed To Alter Expectancies And Alcohol Use In First Year College Students, Amy Schreiner
Evaluation Of A Digitally-Automated Alcohol Curriculum Designed To Alter Expectancies And Alcohol Use In First Year College Students, Amy Schreiner
Electronic Theses and Dissertations
High-risk alcohol consumption remains a primary public health concern for students on college campuses. In response to this concern the National Advisory Council of the National Institute on Alcohol Abuse and Alcoholism created a task force to identify and recommend strategies to aid college administrators in implementing effective alcohol programming at their institutions. While most administrators report being aware of these recommendations, many have not successfully implemented empirically supported interventions on their campuses. One significant barrier is the cost and difficulty of training and hiring skilled staff to implement these interventions. Of the strategies identified as effective, challenging alcohol expectancies …
How Many Are Out There? A Novel Approach For Open And Closed Systems, Zia Rehman
How Many Are Out There? A Novel Approach For Open And Closed Systems, Zia Rehman
Electronic Theses and Dissertations
We propose a ratio estimator to determine population estimates using capture-recapture sampling. It's different than traditional approaches in the following ways: (1) Ordering of recaptures: Currently data sets do not take into account the "ordering" of the recaptures, although this crucial information is available to them at no cost. (2) Dependence of trials and cluster sampling: Our model explicitly considers trials to be dependent and improves existing literature which assumes independence. (3) Rate of convergence: The percentage sampled has an inverse relationship with population size, for a chosen degree of accuracy. (4) Asymptotic Attainment of Minimum Variance (Open Systems: (=population …
Integral Projection Models Reveal Interactive Effects Of Biotic Factors And Disturbance On Plant Demography, Matthew Tye
Integral Projection Models Reveal Interactive Effects Of Biotic Factors And Disturbance On Plant Demography, Matthew Tye
Electronic Theses and Dissertations
Understanding factors limiting population growth is crucial to evaluating species persistence in changing environments. I used Integral Projection Models (IPMs) to elucidate the role of biotic interactions and disturbance on population growth rate in two plants: Helianthemum squamatum, a perennial endemic to gypsum habitats in central Spain, and Liatris ohlingerae, a long-lived perennial endemic to the Lake Wales Ridge of central Florida. In H. squamatum, there was a strong positive effect of trampling in the site with the highest plant density and moderate positive effects of seed addition in the site with the lowest plant density. Differences in treatment effectiveness …
A Comparative Study Of The Effect Of Block Scheduling And Traditional Scheduling On Student Achievement For The Florida Algebra 1 End-Of-Course Examination, Arthur Underwood
A Comparative Study Of The Effect Of Block Scheduling And Traditional Scheduling On Student Achievement For The Florida Algebra 1 End-Of-Course Examination, Arthur Underwood
Electronic Theses and Dissertations
The focus of this research was on the effect of school schedules on student achievement for ninth-grade students in a Florida school district. Data were collected from two central Florida high schools from the 2011-2012 and 2012-2013 school years. Five one-way analyses of covariance (ANCOVA) were performed to ascertain if there was any interaction between school schedules and student achievement. Examined were the interactions (a) between schedule and schools, (b) schedule and male students, (c) schedule and female students, (d) schedule and Black students, and (e) schedule and Hispanic students. The independent variable, school schedule, consisted of two levels: traditional …
A Professional Learning Community Design: Using Close Reading Techniques To Improve The U.S. History Comprehension, Maureen Tinsley
A Professional Learning Community Design: Using Close Reading Techniques To Improve The U.S. History Comprehension, Maureen Tinsley
Electronic Theses and Dissertations
This dissertation in practice presents a research-based model for staff development utilizing the elements of a professional learning community. The focus of this problem of practice was determined through an analysis of one high school's reading data indicating that 36% of the student body was reading below grade level according to the state assessment test for reading. Researchers have noted that reading demands for college and careers have increased (Alliance for Excellent Education, 2011; Barton, 2000; Common Core State Standards, 2014). If students do not develop reading proficiency to graduate with a high school diploma, they are at risk of …
In Vitro Characterization Of Unmodified And Pyroglutamylated Alzheimer's Amyloid Beta Peptide, Jason Matos
In Vitro Characterization Of Unmodified And Pyroglutamylated Alzheimer's Amyloid Beta Peptide, Jason Matos
Electronic Theses and Dissertations
Plaques of amyloid β peptide (Aβ) are a hallmark trait of Alzheimer’s disease (AD). However, the precise role of Aβ aggregates is not well understood. Recent studies have identified that naturally occurring N-terminal truncation and pyroglutamylation of Aβ significantly increases its neurotoxicity by an unknown mechanism. Content of pyroglutamylated Aβ (pE-Aβ) in AD brains has been shown to reach up to 50% of total Aβ. Modified pE-Aβ co-aggregates with Aβ by a seeding mechanism and forms structurally distinct and highly toxic oligomers. We studied structural transitions of the full-length Aβ1-42, its pyroglutamylated form AβpE3-42, their 9:1 (Aβ1-42/AβpE3-42) and 1:1 molar …
Effects Of Mechanical Habitat Disturbance On The Diversity And Network Structure Of Plant-Bee Interaction Networks In Central Florida, Karlie Carman
Effects Of Mechanical Habitat Disturbance On The Diversity And Network Structure Of Plant-Bee Interaction Networks In Central Florida, Karlie Carman
Electronic Theses and Dissertations
Ecological interactions within a community shape the structure of ecosystems and influence ecosystem function. Plant-pollinator interactions exist as mutualistic exchange networks that may collapse as habitat loss occurs, thereby threatening the overall health of an ecosystem. Understanding the impacts of human-mediated habitat disturbance on ecological interactions is therefore crucial for conservation efforts. Archbold Biological Station (ABS) in Venus, Florida contains over 2000 hectares of protected Florida scrub habitat nested within a human-dominated environment that is threatened by anthropogenic habitat disturbance. In past studies, over 113 bee species and 157 associated host plants, many endemic to the Lake Wales Ridge, have …
Effects Of Biotic Interactions On Coastal Wetland Communities With Applications For Restoration, Melinda Donnelly
Effects Of Biotic Interactions On Coastal Wetland Communities With Applications For Restoration, Melinda Donnelly
Electronic Theses and Dissertations
Coastal wetland plants serve as ecological engineers in a physiologically stressful environment and the loss of coastal wetlands can cause negative effects throughout the estuarine system. Due to increased degradation of coastal habitats worldwide, interest in restoration has increased around the world. An understanding of the biotic processes affecting species distribution and diversity is critical for future conservation, management, and restoration of coastal wetlands. The purpose of my study was to test the effects of biotic interactions on native coastal wetland plants and determine how these interactions may be incorporated into current and future restoration projects. I had three primary …
Admission Criteria For Schools Of Business: Common Prerequisites And Academic Performance In Upper-Level Business Coursework, Helen Becker
Admission Criteria For Schools Of Business: Common Prerequisites And Academic Performance In Upper-Level Business Coursework, Helen Becker
Electronic Theses and Dissertations
Schools of business within the Florida State University system have state-mandated common prerequisites that students are required to complete prior to formal admission into baccalaureate business programs. As such, the common prerequisites serve as minimum admission requirements for schools of business in the state of Florida. This study sought to determine the ability of these discipline specific admission criteria to predict academic performance in upper-level business coursework. This study looked at existing data for 860 students in the College of Business Administration at the University of Central Florida. Findings of the study demonstrate that there is a positive and moderate …
Application Of Two-Photon Absorbing Fluorene-Containing Compounds In Bioimaging And Photodyanimc Therapy, Xiling Yue
Application Of Two-Photon Absorbing Fluorene-Containing Compounds In Bioimaging And Photodyanimc Therapy, Xiling Yue
Electronic Theses and Dissertations
Two-photon absorbing (2PA) materials has been widely studied for their highly localized excitation and nonlinear excitation efficiency. Application of 2PA materials includes fluorescence imaging, microfabrication, 3D data storage, photodynamic therapy, etc. Many materials have good 2PA photophysical properties, among which, the fluorenyl structure and its derivatives have attracted attention with their high 2PA cross-section and high fluorescence quantum yield. Herein, several compounds with 2PA properties are discussed. All of these compounds contain one or two fluorenyl core units as part of the conjugated system. The aim of this dissertation is to discuss the application of these compounds according to their …
Establishment Of Methods For Isolation Of Pnmt+ Cardiac Progenitor Cells, Namita Varudkar
Establishment Of Methods For Isolation Of Pnmt+ Cardiac Progenitor Cells, Namita Varudkar
Electronic Theses and Dissertations
Cardiovascular disease is the leading cause of death in the United States. Millions of patients suffer each year from endothelial dysfunction and/or debilitating myocardial damage resulting in decreased quality of life and increased risk of death or disablement. Current pharmacological approaches are only partly effective at treating cardiovascular disease, and hence, better strategies are needed to provide significant improvements in treatment options. Cardiac stem/progenitor cells have the potential to regenerate myocardial tissue and repair damaged heart muscle. There are many different types of cardiac progenitor cells, and each may have certain unique properties and characteristics that would likely be useful …
Genetic And Biochemical Characterization Of The Roles Of Two Putative Purine Transporters In The Infectious Cycle Of Borrelia Burgdorferi, Sunny Jain
Electronic Theses and Dissertations
Lyme disease, the most common tick borne disease in United States, is caused by the bacterial pathogen Borrelia burgdorferi. In nature, B. burgdorferi exists in an enzootic infectious cycle between an arthropod vector and mammalian hosts. Identification and characterization of the genes essential for B. burgdorferi survival throughout its infectious cycle is an important step toward understanding the molecular mechanisms involved in B. burgdorferi pathogenesis. B. burgdorferi contains a small genome, which lacks the genes encoding for the enzymes required for de novo synthesis of amino acids, fatty acids and nucleic acid precursors. Therefore, the spirochete is dependent upon the …
Unintended Outcomes Of Farmers' Adaptation To Climate Variability: Deforestation And Conservation In Calakmul And Maya Biosphere Reserves, Claudia Rodriguez-Solorzano
Unintended Outcomes Of Farmers' Adaptation To Climate Variability: Deforestation And Conservation In Calakmul And Maya Biosphere Reserves, Claudia Rodriguez-Solorzano
Dartmouth Scholarship
Minimizing the impact of climate change on farmer livelihoods is crucial, but adaptation efforts may have unintended consequences for ecosystems, with potential impacts on farmers’ welfare. Unintended outcomes of climate adaptation strategies have been widely discussed, however, empirical exploration has been neglected. Grounded in scholarship on climate adaptation, environmental governance, social–ecological systems, and land-use change, this paper studies whether farmers’ climate adaptation contributes to deforestation or forest conservation. The paper draws on interviews with 353 farmers from 46 communities in Calakmul Biosphere Reserve in Mexico and Maya Biosphere Reserve in Guatemala. Farmers in the area of study have implemented adaptation …
Serum C-X-C Motif Chemokine 13 Is Elevated In Early And Established Rheumatoid Arthritis And Correlates With Rheumatoid Factor Levels, Jonathan D. Jones, B. Jonell Hamilton, Gregory J. Challener, Artur J. De Brum-Fernandes
Serum C-X-C Motif Chemokine 13 Is Elevated In Early And Established Rheumatoid Arthritis And Correlates With Rheumatoid Factor Levels, Jonathan D. Jones, B. Jonell Hamilton, Gregory J. Challener, Artur J. De Brum-Fernandes
Dartmouth Scholarship
We hypothesized that serum levels of C-X-C motif chemokine 13 (CXCL13), a B-cell chemokine, would delineate a subset of rheumatoid arthritis (RA) patients characterized by increased humoral immunity.
Howard Elman's Farewell, Ernest Hebert
Howard Elman's Farewell, Ernest Hebert
Dartmouth Scholarship
Part Falstaff, part King Lear, but all American, Howard Elman was a fifty-something workingman when he burst onto the literary scene in The Dogs of March, the first novel of the Darby Chronicles. Now in this, its seventh installment, the Darby constable is an eighty-something widower who wants to do "a great thing" before he motors off into the sunset.
Maybe Howard achieves this goal, but he manages it in strange, wonderful, and dangerous ways. On his quest he's aided, abetted, hindered, and befuddled by his middle-aged children, his hundred-year-old hermit friend Cooty Patterson, a voice in his head, and …
Let-7 Microrna-Binding-Site Polymorphism In The 3′Utr Of Kras And Colorectal Cancer Outcome: A Systematic Review And Meta-Analysis, Scott M. Langevin, Brock C. Christensen
Let-7 Microrna-Binding-Site Polymorphism In The 3′Utr Of Kras And Colorectal Cancer Outcome: A Systematic Review And Meta-Analysis, Scott M. Langevin, Brock C. Christensen
Dartmouth Scholarship
There is a small but growing body of literature regarding the predictive utility of a Let-7 microRNA-binding-site polymorphism in the 30 -untranslated region (UTR) of KRAS (KRAS-LCS6) for colorectal cancer outcome, although the results are conflicting. We performed a review and meta-analysis in an attempt to better clarify this relationship. A PubMed search was conducted to identify all studies reporting on KRAS let-7 microRNA-binding site polymorphism (LCS6; rs61764370) and colorectal cancer outcome. Hazard ratios (HR) and corresponding 95% confidence intervals (CI) were extracted or estimated from each manuscript. Log HRs and log CIs were combined across studies using the inverse-variance …
For The Birds: Wind Energy, Dead Eagles, And Unwelcome Surprises, Sam Panarella
For The Birds: Wind Energy, Dead Eagles, And Unwelcome Surprises, Sam Panarella
Faculty Law Review Articles
Wind turbines kill birds. A lot of birds. You would be hard pressed to find someone who is happy with that fact, including anyone in the wind energy development community. But until and unless there are technological advances in wind turbine design that eliminate their deadly impact on birds, it is something we must accept. Of course, acceptance does not and should not mean issuing a blank check to wind energy developers to wantonly injure birds. To do so would violate both the spirit and letter of a host of environmental laws that have at their core a stubborn insistence …
Bipolar Nanosecond Electric Pulses Are Less Efficient At Electropermeabilization And Killing Cells Than Monopolar Pulses, Bennett L. Ibey, Olga N. Pakhomova, Caleb C. Roth, Shu Xiao, Karl Schoenbach, Andrei G. Pakhomov
Bipolar Nanosecond Electric Pulses Are Less Efficient At Electropermeabilization And Killing Cells Than Monopolar Pulses, Bennett L. Ibey, Olga N. Pakhomova, Caleb C. Roth, Shu Xiao, Karl Schoenbach, Andrei G. Pakhomov
Bioelectrics Publications
Multiple studies have shown that bipolar (BP) electric pulses in the microsecond range are more effective at permeabilizing cells while maintaining similar cell survival rates as compared to monopolar (MP) pulse equivalents. In this paper, we investigated whether the same advantage existed for BP nanosecond-pulsed electric fields (nsPEF) as compared to MP nsPEF. To study permeabilization effectiveness, MP or BP pulses were delivered to single Chinese hamster ovary (CHO) cells and the response of three dyes, Calcium Green-1, propidium iodide (PI), and FM1-43, was measured by confocal microscopy. Results show that BP pulses were less effective at increasing intracellular calcium …