Digital Commons Network^™

Characterization Of Small Conductance Calcium-Activated Potassium Channels In A Human Lens Epithelium Cell Line (B3), Wedad Ali Saqar

Browse all Theses and Dissertations

Small conductance calcium-activated potassium (SK) channels belong to the family of K+ channels. They play a significant role in cell volume regulation and in lens homeostasis, and thus are associated with cataract formation. This study was designed to demonstrate the presence of the four different isoforms of SK channels (SK1, SK2, SK3, and SK4) in human lens epithelial (HLE)-B3 cell line using Western blot (WB), immunofluorescence (IF), and reverse transcriptase coupled to the polymerase chain reaction (RT-PCR). Cataract formation involves SK-mediated K+ loss and gain of Na+ ions by lens cells. Further, it has been shown in previous studies that …

An Algorithm To Estimate Unsteady And Quasi-Steady Pressure Fields From Velocity Field Measurements, John O. Dabiri, Sanjeeb Bose, Brad J. Gemmell, Sean Colin, John H. Costello

Arts & Sciences Faculty Publications

We describe and characterize a method for estimating the pressure field corresponding to velocity field measurements such as those obtained by using particle image velocimetry. The pressure gradient is estimated from a time series of velocity fields for unsteady calculations or from a single velocity field for quasi-steady calculations. The corresponding pressure field is determined based on median polling of several integration paths through the pressure gradient field in order to reduce the effect of measurement errors that accumulate along individual integration paths. Integration paths are restricted to the nodes of the measured velocity field, thereby eliminating the need for …

Bending Rules For Animal Propulsion, Kelsey N. Lucas, Nathan Johnson, Wesley T. T. Beaulieu, Eric Cathcart, Gregory Tirrell, Sean Colin, Brad J. Gemmell, John O. O. Dabiri, John H. Costello

Arts & Sciences Faculty Publications

Animal propulsors such as wings and fins bend during motion and these bending patterns are believed to contribute to the high efficiency of animal movements compared with those of man-made designs. However, efforts to implement flexible designs have been met with contradictory performance results. Consequently, there is no clear understanding of the role played by propulsor flexibility or, more fundamentally, how flexible propulsors should be designed for optimal performance. Here we demonstrate that during steady-state motion by a wide range of animals, from fruit flies to humpback whales, operating in either air or water, natural propulsors bend in similar ways …

Structural And Functional Support In The Prediction Of Smoking Cessation In Caregivers Of Children With Asthma, Erin M. Tooley, Andrew Busch

Arts & Sciences Faculty Publications

Caregivers of children with asthma smoke at a rate similar to the general population. Research on the relative importance of structural or functional social support in smoking cessation has been mixed. Participants were smokers (N=154) who were caregivers of children with asthma. Both functional (perception of social support measured by the Interpersonal Support Evaluation List) and structural social support (living with another smoker, partner status, and the proportion of smoking friends) were measured at baseline. Participants received an asthma-education and smoking cessation intervention based on Motivational Interviewing. Biochemically-verified abstinence was assessed at 6-months post treatment. Hierarchical logistic regression analyses indicated …

Investigating Dna Barcoding Potentials And Genetic Structure In Ozobranchus Spp. From Atlantic And Pacific Ocean Sea Turtles, Triet Minh Truong

Browse all Theses and Dissertations

The Ozobranchidae family is the smallest and least studied hirudinean taxon. Our research includes the largest molecular dataset yet reported for marine ozobranchids (Ozobranchus margoi and Ozobranchus branchiatus) with the most number of documented turtle hosts (57) from the Atlantic and Pacific Oceans to date of any marine turtle epibiont study. Turtle species sampled in this study include loggerheads (Caretta caretta), hawksbill (Eretmochelys imbricata), olive ridley (Lepidochelys olivacea), and green turtles (Chelonia mydas). Phylogenetic analyses of mitochondrial (COI) and nuclear ribosomal (18S and 28S) genes all support the monophyly of marine Ozobranchidae leeches with speciation occurring over an extensive period …

Destruction Of Chlorinated Hydrocarbons By Zero-Valent Zinc And Bimetallic Zinc Reductants In Bench-Scale Investigations, Christopher Scott Cushman

Browse all Theses and Dissertations

Many remediation technologies have been developed to treat extensive subsurface contamination by chlorinated hydrocarbons (CHCs). One such technology is a permeable reactive barrier (PRB), which contains reactive media that acts as an electron donor in order to reduce CHCs. Extensive effort has been placed on finding the most suitable reactive media in PRBs, with zero-valent iron (ZVI) being the most commonly utilized media. However, zero-valent zinc (ZVZ) is a promising replacement for ZVI in PRBs as it will more readily donate electrons, resulting in more rapid degradation of CHCs. In addition, amending a secondary/catalytic metal to primary metal surface can …

Socs1/Socs3 Expression And Virus Replication Of Denv2 And Hsv-1 In Cytokine-Polarized Subsets Of Raw 264.7 Macrophages, Nagarjuna Reddy Cheemarla

Browse all Theses and Dissertations

Cytokine activation of macrophages leads to "macrophage polarizatio" The M1 subset is polarized by exposure to lipopolysaccharide (LPS) and interferon-gamma (IFN-γ) and M2 polarized cells develop after exposure to interleukin-4 (IL-4) or IL-13. In this study, the RAW 264.7 murine macrophage cell line was used to study the effect herpes simplex virus-Type I (HSV-1) and Dengue virus serotype-2 (DENV2) infections have on cultures of unpolarized (M0) or polarized subsets. The macrophage subsets were characterized using cluster of differentiation markers CD14 and CD86. Uninfected M1 macrophages showed up regulation in expression of the co-stimulatory factor B7.2 (CD86) compared to expression by …

The Camkkβ Inhibitor Sto-609 Causes Artefacts In Ca2+ Imaging And Selectively Inhibits Bkca In Mouse Carotid Body Type I Cells, Jennifer G. Jurcsisn

Browse all Theses and Dissertations

It has previously been reported that AMP-activated protein kinase (AMPK) may be critical for hypoxic chemotransduction in carotid body type I cells (Wyatt et al, 2007). This study sought to determine the importance of the regulatory upstream kinase of AMPK, CamKKβ, in the acute response to hypoxia in isolated mouse type I cells.

Initial studies indicated several previously unreported artefacts associated with using the CamKKβ inhibitor STO-609 and calcium imaging techniques. Strong STO-609 autofluorescence meant that Fura-2 could not be used and X-Rhod-1 imaging revealed that STO-609 quenched fluorescence even in the absence of intracellular Ca2+. The whole-cell configuration of …

A Feminist Oversight: The Reproductive Rights Of Women In Prisons, Rebecca Burgan

Browse all Theses and Dissertations

Historically, the value of a woman has been based on her ability to produce healthy and successful (male) children, leaving little room for worth based on intelligence or other achievements. Second wave feminists made significant strides for women's reproductive choices, acknowledging that motherhood is not the only way for a woman to have fulfillment or value. While women throughout the United States have more access, opportunity, and choice, women in United States prisons have not benefited from the same advancements in reproductive freedom. The denial of women's reproductive freedoms in prison can be attributed to the high costs of pregnancy …

American Insanity: The Demise Of The Elite And A Critical/Historical Analysis Of The Dsm, Tiffany B. Hunter

Browse all Theses and Dissertations

The International Classification of Diseases, Sixth Edition (ICD-6; World Health Organization, 1949) is a universal health care management tool for examining the pathology of diseases and how to properly diagnose and treat said diseases. The birth of the first edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-I; American Psychiatric Association, 1952) is the product of the ICD and the American Medico-psychological Association and first delivered shortly after the end of the Second World War. As per request of the American Armed Forces, which were ill-equipped to manage the surge of mental disorders after the war, the need …

A Chemical Free Approach For Increasing The Biochemical Surface-Enhanced Raman Spectroscopy (Sers)-Based Sensing Capabilities Of Colloidal Silver Nanoparticles, Kevin Michael Dorney

Browse all Theses and Dissertations

The unique optoelectronic properties of silver nanoparticles (AgNPs) have led to their explosive use in a multitude of both research and industrial settings in recent years. Localized surface plasmon resonance (LSPR) exhibited by AgNPs has been exploited extensively as a nano-scale probe in a variety of spectroscopic detection modalities, particularly in surface-enhanced Raman spectroscopy (SERS). The SERS effect is highly dependent upon the LSPR interaction with optical laser frequencies, thus optimization of LSPR via specific control of AgNP dimensionality and composition of the surrounding medium is vital for increasing the efficacy of SERS-based nano-sensing. This work aimed to augment LSPR …

Thermal Properties Of Magnetic Nanoparticles In External Ac Magnetic Field, Anna Beata Lukawska

Browse all Theses and Dissertations

This work studies thermal properties of magnetic nanoparticles in an external ac magnetic field. Dried iron and cobalt nanoparticles were prepared by thermal decomposition of iron pentacarbonyl (Fe(CO)5) and dicobalt octacarbonyl (Co2(CO)8), triscobalt nona(carbonyl)chloride (Co3(CO)9Cl), or tetracaobalt dodecacarbonyl (Co4(CO)12) [1]. The samples had different mean diameters: 5.6 - 21.4 nm for iron and 6.5 - 19.4 nm for cobalt. Each sample was exposed to ac magnetic field and the increase in temperature of the sample was measured. Results were analyzed to find the critical diameters for the transitions from multi-domain to single-domain and from single-domain to superparamagnetic regime. The nanoparticles …

Early Increase Of Cd11c In Human Monocyte-Derived Dendritic Cells In The Presence Of A/California/07/2009 (H1n1pdm), Amber M. Braddock

Browse all Theses and Dissertations

Influenza A continues to cause significant morbidity and mortality. Vaccine production time, viral antigenic variability and low vaccine efficacy contribute to the annual pandemics and seasonal epidemics. A better understanding of human immune responses to influenza A could result in more effective vaccines. Dendritic cells (DCs) play an important role in the initiation of immune responses. Populations of B lymphocytes secrete protective antibodies but the role of different populations of B cells in the protective response is unknown. In this thesis we developed assays to determine subclasses of influenza specific antibodies. Mononuclear cells were assessed using flow cytometry to identify …

The Modulatory Role Of Circulating Microvesicles In Endothelial Progenitor Cell Function Is Altered In T2dm, Hala Mustafa Ammar

Browse all Theses and Dissertations

Circulating microvesicles (cMVs) are the extracellular MVs released from the cells in the blood and on the vascular wall. Our previous study demonstrates that cMVs of diabetic mouse are detrimental to endothelial progenitor cells (EPCs), which are known to be very important for maintaining normal endothelial function and structure. In this study, we compared the levels of circulating EPCs and EPC-derived MVs (EPC-MVs) in diabetic and healthy subjects. Also, the migration ability, apoptosis rate and reactive oxygen species (ROS) production of EPCs cultured from diabetic and healthy subjects were determined. More importantly, we evaluated whether cMVs from healthy subjects (ch-MVs) …

Fear Of Discrimination And Leveraging Of Leadership Experience In Individuals Of Lgbtq Organizations, Megan Brianne Morris

Browse all Theses and Dissertations

Awareness of the Lesbian, Gay, Bisexual, Transgender, and Queer (LGBTQ) community has exposed these individuals to unequal treatment in the workplace. One area where this mistreatment occurs is in the job application process. As a result, it is important to examine the job seeking behaviors of the LGBTQ community that are associated with this process. Student leaders of LGBTQ-focused groups face a unique challenge in the application process in regard to whether they should include their leadership experience on their resume, possibly exposing themselves to discrimination and bias. Only one study, to current knowledge, has focused on resume construction behavior …

Study On The Vortex Wake Of An Airfoil Equipped With Flexible Trailing Edge Fringes, Zhengkai He

Browse all Theses and Dissertations

With the inspiration of owl's silent flight, a traditional airfoil S833 equipped with flexible fringes on the trailing edge is investigated through numerical simulation and experiments in a wind tunnel. The newly constructed airfoil is modeled and numerically investigated. An incompressible, 2D and viscous flow solver in the Computational Fluid Dynamics (CFD) software FLUENT is utilized to conduct the numerical simulation on the vortex flow feature in the wake of the airfoil. A User Defined Function code was applied to generate the defined motion of flexible fringes. The effects of the flapping frequency of the fringe and the deformation pattern …

Hierarchical Porous Structures Functionalized With Silver Nanoparticles: Adaptation For Antibacterial Applications, Anil Kumar Karumuri

Browse all Theses and Dissertations

For a large number of applications involving surface interactions, there is an ever growing demand for materials with high specific surface area (surface area to volume ratio). Porous materials and nanomaterials offer higher specific surface area (SSA) each with their respective advantages and disadvantages. This research program is focused on bringing the two approaches together to create truly "hierarchical" materials that combine advantages of both while minimizing their disadvantages. In this study, various carbon based porous geometries were fabricated by grafting carbon nanotubes (CNT) onto highly porous reticulated carbon foams. These structures were further modified by functionalizing metal nanoparticles such …

Development Of A Co-Culture System To Mimic The Transfection Of Hsv-1 From Keratinocytes To Neuronal Cells, David A. Dixon

Browse all Theses and Dissertations

To more closely mimic the in vivo progression of HSV-1 a novel in vitro method was created. In this study HEL-30 keratinocytes were infected with a 0.1 MOI of HSV-1 and plated unto a membrane with 8μm diameter pores. The membrane insert was then placed into culture wells that contained Neuro-2a cells (N2A). The neurites from the neuronal cells made cell-to-cell contact with the infected HEL-30 cells and the virus was transmitted into the neuronal cells. To determine if infection occurred in the N2A cells the cells were lysed and the lysate incubated with Vero cells to titrate virus plaque …

White Blood Cell Counts, Parasite Prevalence, And Plasma Cortisol Levels Of Dogs In A County Animal Shelter: Changes Over Days And Impact Of A Program Of Repeated Human Interaction, Emily S. Dudley

Browse all Theses and Dissertations

Animal shelter housing is highly stressful for a dog, compromising welfare and leading to undesirable behaviors and unknown health consequences. We documented the changes in circulating numbers of white blood cells, plasma cortisol, and fecal parasite shedding of dogs housed for 10 days at a county animal shelter. White blood cell changes were most prominent on Day 10 after arrival to the shelter. Changes included increased total leukocytes, mature neutrophils, and lymphocytes, with less consistent increases in monocytes and neutrophil to lymphocyte ratio (N:L). Fecal parasite shedding was elevated and not affected by day. Cortisol levels of shelter dogs declined …

Exploring How Middle School Students' Epistemologies In Practice Change Across Time With Varying Content Areas And Knowledge Product Contexts, Mackenzie Evan English

Browse all Theses and Dissertations

Recently, the National Research Council developed A Framework for K-12 Science Education (NRC, 2012), to support a new vision of science education. The Framework (2012) focuses on three integrated dimensions--disciplinary core ideas, crosscutting concepts, and scientific and engineering practices which is different than traditional ways of learning science. I focused on students' engagement in scientific practices, attention to epistemological practices (EIP) that guide students' construction, evaluation and revision of knowledge products. I examined how students EIP's changed over time and across contexts with respect to different knowledge product types (models and explanations). I present data from 103, 6th grade students …

Evaluation Of Functional Near Infrared Spectroscopy (Fnirs) For Assessment Of The Visual And Motor Cortices In Adults, Brenna Giacherio

Browse all Theses and Dissertations

Introduction: Functional near-infrared spectroscopy (fNIRS) is a relatively young technique in the field of medical imaging. As such, it has yet to be widely implemented for clinical use, despite its promising advantages. However, unlike fMRI-its much bulkier and costly counterpart-fNIRS has yet to be proven as a standalone imaging tool within a clinical setting, particularly that of ophthalmology or physical therapy.

Methods: Ten healthy young adults (23.8 ± 4.8 years) participated in the study. Activation of the visual cortex was achieved utilizing various reversing checkerboard stimuli across three data collection sessions for each participant. Further, activation of the motor cortex …

Effects Of Delayed Pharmacological Treatment And Limb Rehabilitation On Infarct Size And Functional Recovery After Stroke, Maria Helen Harley Balch

Browse all Theses and Dissertations

Previous studies show a fluoxetine/simvastatin drug combination successfully reduced infarct size and increased functional recovery when administered 20-26 hours post stroke. This project tested the hypothesis that earlier drug delivery at 6-12 hours post stroke will improve functional recovery and decrease infarct size, and that limb rehabilitation will improve functional recovery.

Pre-stroke function was determined in rats, a stroke was induced, and daily treatment began 6-12 hours post stroke. Baseline functional deficit was established, and additional testing over 90 days monitored functional recovery. Limb rehabilitation was provided for designated animals, and brain analysis measured infarct size.

In animals with no …

Stop Cheating!: An Evaluation Of A Scientific Integrity Writing Strategy In General Chemistry I, Michelle L. Edwards

Browse all Theses and Dissertations

Cheating in America has become an epidemic, which has unfortunately spread to academia. Researchers have long been interested in the trends in cheating behavior and the factors that influence these trends among undergraduate college students. Though cheating can take on many forms, in science, there is a deep concern about scientific misconduct related to research. Because training for future scientists begins in undergraduate courses, this study investigated the use of scientific integrity writing strategy in General Chemistry I at Wright State University. The goal of the study was to determine if providing scientific integrity and ethics training, while teaching students …

Routes To N-Heterocycle Functionalized Poly(Arylene Ether Sulfone)S, Jesse L. Picker

Browse all Theses and Dissertations

The synthesis of poly(arylene ether sulfone)s, with a pendant aryl iodide, was achieved via nucleophilic aromatic substitution (NAS) polycondensation of 3-iodo-3',5'-difluoro-diphenylsulfone. The polymer series was subjected to amino acid promoted, copper catalyzed coupling reactions with various N-heterocycles. The resulting polymers became crosslinked and insoluble in any organic solvents. The iodo monomer was then subjected to an amino acid promoted, copper catalyzed coupling reaction with carbazole. The successful synthesis of 3'-(9-carbazole) 3,5-difluorodiphenylsulfone is reported. A series of PAES containing a carbazole substituent were synthesized and characterized. The polymers were characterized by a combination of NMR spectroscopy, SEC, TGA, DSC, UV-Vis spectroscopy. …

Assortative Fertilization In The Elegans-Group Of Caenorhabditis, Sara Rose Seibert

Browse all Theses and Dissertations

Assortative fertilization refers to the species-specific interactions between sperm and oocytes that affect the success of fertilization. One type of interaction is chemotaxis of sperm to oocytes. In Caenorhabditis elegans, amoeboid sperm must crawl along the uterine lining towards the spermathecae in response to oocyte-derived prostaglandin signals for fertilization. This chemotactic signaling system likely operates in other species of the Elegans-Group of Caenorhabditis as sperm of C. briggsae and C. remanei do localize to the C. elegans spermathecae. In this project the impacts of species-specific chemotaxis on fertilization and female fecundity were assessed. To accomplish this, the localization of …

Investigating Anatomical And Molecular Aspects Of Proprioceptive Sensory Neuron Diversity Using A Transgenic Mouse Model, Martha Jean Sonner

Browse all Theses and Dissertations

Muscle spindles (MS) and Golgi tendon organs (GTOs) are encapsulated, stretch-activated sensory receptors housed within skeletal muscles, yet their functional contributions to proprioceptive monitoring of limb movements are quite different. For example, MS are responsible for detecting stretch of muscle fibers, and GTOs provide feedback regarding muscle tension during contraction. Peripheral axons extending from proprioceptive sensory neurons (PSNs) in the dorsal root ganglia supply MS and GTOs. MS are innervated by Group Ia and II sensory fiber endings, while GTOs are innervated by a single Group Ib sensory fiber. The developmental processes guiding axons from PSNs to their targets and …

Biogeochemistry Of Sulfur Isotopes In Crystal Lake, Clark County, West-Central Ohio, Amanda Lynn Meyer

Browse all Theses and Dissertations

Crystal Lake showed distinct biogeochemical patterns, which were governed by thermal stratification, photosynthesis, and sulfur redox processes. Field parameter measurements and water and Planktothrix rubescens samples were collected in May, August, and October, 2013 at the deepest point in the lake, to better understand these processes. At the metalimnion-hypolimnion boundary, P. rubescens produced chlorophyll and turbidity maxima. Photosynthesis produced oxygen-rich and nutrient-poor surface waters. The decay of organic matter produced anoxic, nutrient-rich water in the hypolimnion. Sulfate concentrations were high in the epilimnion and metalimnion, with a maximum at the layer of P. rubescens, and decreased with depth in the …

Mercury Distributions And Cycling In The North Atlantic And Eastern Tropical Pacific Oceans, Katlin L. Bowman

Browse all Theses and Dissertations

The distribution of mercury (Hg) in the ocean is complex as a result of in situ chemical transformations and inputs from natural and anthropogenic sources. Within the ocean, inorganic Hg is methylated to monomethylmercury (MMHg), which bioaccumulates and biomagnifies in marine food webs and poses a health risk to humans who eat fish. The biogeochemistry of Hg in the ocean has been studied for decades, however, recently improved sampling and analytical techniques have allowed for an enhanced understanding of global distributions of different Hg species. This dissertation uses a newly developed method for the analysis of MMHg that improves detection …

Environmental Factors Affecting Methylmercury In Fish Of The Laurentian Great Lakes Region, Joel Harvey

Browse all Theses and Dissertations

Monomethylmercury (MMHg) can accumulate in fish to concentrations that pose a threat to the health of fish, piscivorous organisms, and humans who eat fish. A variety of environmental factors have been hypothesized to influence either the methylation of inorganic Hg or the bioaccumulation and magnification of MMHg. This study investigates the influence of selected environmental factors on MMHg concentrations in freshwater fish across a regional scale, most of the U.S. portion of Laurentian Great Lakes region. Fish MMHg was correlated with proton deposition, sulfate deposition, nitrate deposition, mercury deposition, pH, watershed area, and Secchi depth. Only proton deposition was positively …

Expression, Characterization, Cloning And Functional Impact Of Slc12a5 In The Endocrine Pancreas, Shams Kursan

Browse all Theses and Dissertations

The Slc12a5 gene encodes the neuron-specific electroneutral K+Cl- cotransporter-2 (KCC2), a constitutively active Cl- extruder. In mature neurons, KCC2 functionally predominates over other members of the Slc12a family, particularly the Na+K+2Cl- cotransporter-1 (NKCC1), a ubiquitous Cl- loader. Therefore, KCC2 keeps and maintains the intracellular chloride concentration ([Cl-]i) below values predicted by thermodynamic equilibrium and in doing so, KCC2 plays a key role in neuron physiology: it makes possible the inhibitory action of neurotransmitters such as gamma-aminobutyric acid (GABA) and glycine. Neurons and insulin-secreting pancreatic ß-cells share many genes and functions. Indeed, neuron-specific genes have been also found in ß-cells whereas …