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2014

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Effects Of Tea (Camellia Sinensis) On Antioxidant Potential And Fermentation Characteristics Of Yogurt / Premalatha A/P Muniandy, Premalatha Muniandy Jan 2014

Effects Of Tea (Camellia Sinensis) On Antioxidant Potential And Fermentation Characteristics Of Yogurt / Premalatha A/P Muniandy, Premalatha Muniandy

Student Works (2010-2019)

The present study investigated the effects of green, white and black tea (Camellia sinensis; 2% w/v) on the fermentation of milk and antioxidant potential of yogurt. Each tea (water extract) was added into milk-starter culture mixture and incubation was carried out at 42°C until pH was reduced to 4.5. The yogurts were then refrigerated (4°C) for up to 21 days and samples were analysed for antioxidant potential (diphenyl picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP) and ferrous ion chelating (FIC) assays), pH, titratable acid and viable yogurt bacteria counts. Tea yogurts had higher antioxidant potentials (p < 0.05) than plain yogurt with green tea yogurt (GTY) having the highest FRAP (2.49 - 2.98 mmol Fe2+ E/L) and black tea yogurt (BTY) having the highest FIC (87.50 - 89.87 %) activity throughout the storage period. Both GTY (90.07 - 96.74%) and white tea yogurt (WTY; 89.83 - 96.39%) showed the highest DPPH radical scavenging activity throughout the storage period. The presence of green and black tea water extracts prolonged the milk fermentation time (>270 minutes …

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Separation Of Metallic And Semiconducting Single Walled Carbon Nanotubes Using Aqueous Two-Phase Technique / Malcolm Tang Siong Yii, Siong Yii Malcolm Tang Jan 2014

Separation Of Metallic And Semiconducting Single Walled Carbon Nanotubes Using Aqueous Two-Phase Technique / Malcolm Tang Siong Yii, Siong Yii Malcolm Tang

Student Works (2010-2019)

The presence of metallic single-walled carbon nanotubes (M-SWCNT) in as-prepared single-walled carbon nanotubes (SWCNT) poses a significant hurdle to integrate carbon nanotubes into semiconductor electronics. Here the findings of enriching semiconducting single-walled carbon nanotubes (S-SWCNT) using an aqueous two-phase system (ATPS) is reported. The solution containing as received SWCNT and polyethylene glycol (PEG), dextran, N-methylpyrrolidone (NMP) and water effectively separates into two distinct phases, with S-SWNT dominant in one phase while the M-SWCNT congregates in another phase. The separation process is due to the attraction of amine and S-SWCNT as reported by Chattopadhyay. Micro-Raman spectroscopy and Ultraviolet-Visible spectroscopy results show …

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Phenotypic Characterization Of Amoeboid Forms Of Trichomonas Vaginalis / Kasthuri A/P Jewarethnam, Kasthuri Jewarethnam Jan 2014

Phenotypic Characterization Of Amoeboid Forms Of Trichomonas Vaginalis / Kasthuri A/P Jewarethnam, Kasthuri Jewarethnam

Student Works (2010-2019)

Trichomonas vaginalisis a flagellated protozoan parasite that causes significant health drawbacks to humans, predominantly in women by causing a non-viral sexually transmitted disease known as trichomoniasis. Different morphological properties of T. vaginalis were recognized as the key for infections in humans with trophozoites and pseudocysts as the two notable forms. Recently, amoeboid form was identified in suspension cultures and was reported as an integral life cycle of T. vaginalis. The present study was conducted to study the phenotypic characteristics of amoeboid forms in suspension culture i.e. growth profile, evaluating factors that trigger formation of this shape, staining characteristics and transformational …

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Establishment Of A Real Time Rt-Pcr Assay To Detect Expression Of Selected Genes From Multiple-Drug Resistant Acinetobacter Baumannii / Sanaz Koosha, Sanaz Koosha Jan 2014

Establishment Of A Real Time Rt-Pcr Assay To Detect Expression Of Selected Genes From Multiple-Drug Resistant Acinetobacter Baumannii / Sanaz Koosha, Sanaz Koosha

Student Works (2010-2019)

Acinetobacter baumannii has an important role in nosocomial infections. However, in addition to the respiratory infections caused by this bacterium it also causes other infections, such as, bacteremia, urinary tract infections, meningitis, eye and skin infections. Recently the antibiotic resistance of A. baumannii has increased in respect of almost all of the current antibiotics. The present study focuses on the expression of genes ABAYE0161 (hypothetical protein) and ABAYE3501 (general secretion pathway protein C) in the presence and absence of the antibiotic imipenem. These two genes were chosen from the previous studies concerning the effect of microgravity on the expression of …

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Evaluation Of Shake Flask And Bioreactor Systems On Cell Growth And Regeneration Of Musa Acuminata Cv. Berangan Cell Suspension Culture / Wendy Chin Yi Wen, Chin Yi Wen Wendy Jan 2014

Evaluation Of Shake Flask And Bioreactor Systems On Cell Growth And Regeneration Of Musa Acuminata Cv. Berangan Cell Suspension Culture / Wendy Chin Yi Wen, Chin Yi Wen Wendy

Student Works (2010-2019)

In this study, a semi automated cell suspension culture protocol for Musa acuminata cv. ‘Berangan’ using bioreactor has been established. Flower clusters at the positions of 8 to 10 were the most responsive (70 %) towards embryogenesis. After eight months of culture, embryogenic calli generated from male inflorescences were transferred to liquid suspension medium. Embryogenic cell suspension culture was successfully established after two months of culture in liquid M2 medium. Cell suspension cultures showed optimum cell growth when cultured in the M2 modified liquid medium containing 2 % sucrose with an initial inoculum density of 2 % settled cell volume …

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Identification Of Rb1 Germ-Line Mutations In Patients With Retinoblastoma / Kavimalar A/P Renganathan, Kavimalar Renganathan Jan 2014

Identification Of Rb1 Germ-Line Mutations In Patients With Retinoblastoma / Kavimalar A/P Renganathan, Kavimalar Renganathan

Student Works (2010-2019)

Background: Retinoblastoma (RB1; OMIM#180200) is the most common intraocular tumor in early childhood. The malignancy mainly affects children under five years of age, with a prevalence of 1:15,000 to 1:20,000. RB1 or retinoblastoma susceptibility gene is the gene associated with retinoblastoma and is located on chromosome 13q14.2. The whole coding region of RB1 has 27 exons. The gene encodes a tumor suppressor, the retinoblastoma protein (pRB). Retinoblastoma develops when both alleles of the tumor suppressor gene are inactivated by loss-of-function mutations. The hereditary predisposition to the disease is caused by germ-line mutations in RB1. Since RB1 is a tumor suppressor …

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Studies On Chemical Constituents And Biological Activities Of Nauclea Officinalis And Nauclea Subdita / Liew Sook Yee, Sook Yee Liew Jan 2014

Studies On Chemical Constituents And Biological Activities Of Nauclea Officinalis And Nauclea Subdita / Liew Sook Yee, Sook Yee Liew

Student Works (2010-2019)

The phytochemical studies on two species of Rubiaceae; Nauclea officinalis and Nauclea subdita have been carried out. Twenty one compounds were successfully isolated and purified using several chromatography techniques such as column chromatography (CC), thin layer chromatography (TLC), preparative thin layer chromatography (PTLC), and high performance liquid chromatography (HPLC). The compounds are naucline, nauclefine, naucletine, angustine, angustoline, 3,14-dihydroangustoline, angustidine, subditine, strictosamide, pumiloside, naucleficine, naucleactonin C, harmane, 1,2,3,4-tetrahydro-1-oxo-β-carboline, benzamide, cinnamide, blumenol B, blumenol A, β-sitosterol, stigmast-4-en-3-one and vanillin. Naucline and subditine are new indole alkaloids which were isolated from the bark of Nauclea officinalis and Nauclea subdita respectively. The structure of …

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Metallothionein Gene Expression And Genotoxic Effects Of Heavy Metals On Oreochromis Sp. / Elani Laili Juhari, Juhari Elani Laili Jan 2014

Metallothionein Gene Expression And Genotoxic Effects Of Heavy Metals On Oreochromis Sp. / Elani Laili Juhari, Juhari Elani Laili

Student Works (2010-2019)

Metallothionein is a small, cysteine rich protein that aids in ion homeostasis in a cell. It binds naturally to zinc and also has the tendency to bind to other metals as well if present in the cell. This study was conducted in order to determine the effects of heavy metals exposure on metallothionein expression and other genotoxic effects on the tilapia fish as test subjects. Oreochromis sp. was chosen as the test subject because of the many advantages of its characteristics and it can be easily found in Malaysian rivers. Test subjects were exposed to two types of metals which …

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Phytochemical Studies And The Bioactivities Of Compounds From Ochrosia Oppositifolia, Rauvolfia Reflexa And Actinodaphne Machrophylla / Mehran Fadaeinasab, Mehran Fadaeinasab Jan 2014

Phytochemical Studies And The Bioactivities Of Compounds From Ochrosia Oppositifolia, Rauvolfia Reflexa And Actinodaphne Machrophylla / Mehran Fadaeinasab, Mehran Fadaeinasab

Student Works (2010-2019)

The chemical constituents of Ochrosia oppositifolia, Rauvolfia reflexa (Apocynacea) and Actinodaphne machrophylla (Lauracea) have been studied. The compounds were extracted from the bark and leaves of the plants using n-hexane, dichloromethane and methanol as solvents. The crudes were subjected to extensive chromatographic techniques such as column chromatography (CC), preparative thin layer chromatography (PTLC), and high performance liquid chromatography (HPLC). Structural elucidation was established through several spectroscopic methods, such as 1D-NMR (1H, 13C, DEPT, NOE), 2D-NMR (COSY, NOESY, HMQC, and HMBC), UV, IR, and MS (GCMS, LCMS and HREIMS) and comparison with the published data. In this study, a total of …

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Modelling And Computer Simulation Studies Of Sugar Based Amphiphilic Systems / Sara Ahmadi, Sara Ahmadi Jan 2014

Modelling And Computer Simulation Studies Of Sugar Based Amphiphilic Systems / Sara Ahmadi, Sara Ahmadi

Student Works (2010-2019)

Nowadays, sugar-based amphiphiles are among the most attractive class of compounds to be studied both fundamentally and application-wise. They are one of the main components in cell membranes and play important role in many biological functions. Therefore, understanding the nature of the different liquid crystal phases of these materials is important and conceivable using different experimental techniques, theory and simulation. This thesis focuses on the last two methods to investigate the properties of these highly interesting soft materials. We begin by examining a set of novel glycolipids which has been modified to include crown ethers attached to the sugar ring …

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Utility Of Bats (Chiroptera) As Ecological Indicators In Peninsular Malaysia / Khairunnisa Binti Syaripuddin, Khairunnisa Syaripuddin Jan 2014

Utility Of Bats (Chiroptera) As Ecological Indicators In Peninsular Malaysia / Khairunnisa Binti Syaripuddin, Khairunnisa Syaripuddin

Student Works (2010-2019)

As ecologists we are tasked with studying the effects of environmental changes on ecosystems, but it is impossible to study every species and every interaction. Therefore, a relatively small group of species must be chosen as a proxy for monitoring general patterns of ecological change. Bats might be suitable for the role as ecological indicators because they have high species richness and abundance, represent several distinct feeding guilds, occupy high trophic positions, and perform key ecosystem services such as pollination, seed dispersal, nutrient recycling and arthropod control. A critical prerequisite for an ecological indicator group is stable and accurate species …

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Genetic Diversity Of Gracilaria Changii And Gracilaria Edulis (Gracilariaceae,Rhodophyta) In Malaysian Waters / Yow Yoon Yen, Yoon Yen Yow Jan 2014

Genetic Diversity Of Gracilaria Changii And Gracilaria Edulis (Gracilariaceae,Rhodophyta) In Malaysian Waters / Yow Yoon Yen, Yoon Yen Yow

Student Works (2010-2019)

Gracilaria is the second largest genus of red algae that is widely distributed from the sub-boreal to tropical waters, and some are endemic to the Southeast Asian countries. G. changii (Xia et Abbott) Abbott, Zhang et Xia and G. edulis (Gmelin) Silva are potential species for commercialization in Malaysia as they have high yields of good quality agar with high gel strength for production of agarose and food grade agar. There is no cultivation of Gracilaria changii and Gracilaria edulis in Malaysia and regular sampling at various coastal areas in Peninsular Malaysia has indicated that the wild populations of these …

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Taxonomy And Phylogeny Of Crustose Brown Algae (Phaeophyceae) From Malaysia And Lombok Island, Indonesia / Poong Sze Wan, Sze Wan Poong Jan 2014

Taxonomy And Phylogeny Of Crustose Brown Algae (Phaeophyceae) From Malaysia And Lombok Island, Indonesia / Poong Sze Wan, Sze Wan Poong

Student Works (2010-2019)

Studies on crustose brown algae are relatively few despite a long history of studies conducted since the 1800s, with temperate species forming the bulk of these studies. There is a need for more focus on crustose brown algae particularly in the tropics as they are generally different from those in the temperate regions. These algae received little attention due to their simple appearance (brownish-black spots on rocks) and lack of apparent economic value despite some being the dominant flora of certain habitats. Taxonomic confusion arising from morphological simplicity largely dependent on the reproductive structures and overlap in morpho-anatomical features among …

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Stability, Functionality And Biosafety Of Nicotiana Tabacum Expressing Anti-Cmv Antibodies / Ng Cheah Wei, Cheah Wei Ng Jan 2014

Stability, Functionality And Biosafety Of Nicotiana Tabacum Expressing Anti-Cmv Antibodies / Ng Cheah Wei, Cheah Wei Ng

Student Works (2010-2019)

Mosaic Virus (CMV) is a significant plant pathogen affecting various crops and plants in Malaysia. A single chain variable fragment (scFv) anti-CMV antibody was successfully developed via a scFv library constructed with mRNA from the spleen cells of a CMV coat protein-immunized mouse and transformed by Agrobacterium tumefaciens into tobacco plants (Nicotiana tabacum L. cv. White burley). In this study, the performance of primary transformants and 3 successive generations of Nicotiana tabacum expressing anti-CMV scFv were evaluated. An overall of 20% reduction in seed germination was observed as compared to wild type tobacco. All 4 generations did not exhibit any …

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Chemical Constituents Of Prismatomeris Malayana Ridley And Quantitative Structure Activity Relationship Study On Anti-Inflammatory Agents And Their Analogues / Nor Hayati Abdullah, Nor Hayati Abdullah Jan 2014

Chemical Constituents Of Prismatomeris Malayana Ridley And Quantitative Structure Activity Relationship Study On Anti-Inflammatory Agents And Their Analogues / Nor Hayati Abdullah, Nor Hayati Abdullah

Student Works (2010-2019)

Prismatomeris malayana Ridley of Rubiaceae was investigated for its anti-inflammatory properties by evaluating their inhibition of the hyaluronidase and lipoxygenase activity and of TPA-induced inflammation in mice. The leaf was found to be the most active in the three selected assays. From bioassay guided fractionation and isolation, ursolic acid 60 was isolated and found to be the compound responsible for the hyaluronidase inhibitory activity. The isolation work on the leaves yielded five pentacylic triterpenoids (PTC) (3β-hydroxyurs-12-en-28-oic acid 60 [ursolic acid], 3β, 19, 24-trihydroxyurs-12-en-28-oic acid 61 [barbinervic acid], 3β,23-dihydroxyurs-12-en-28-oic acid 62, 28-O-β-glucopyranosyl-3α,19α,23-trihydroxyurs-12-en-28-oic acid 63 and 3β-hydroxyurs-11-en-13,28-olide 65); three iridoids (asperulosidic acid …

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Synthesis And Characterisation Of Organotin (Iv) Carboxylate Complexes / Kelly Tan Ching Yi, Kelly Ching Yi Tan Jan 2014

Synthesis And Characterisation Of Organotin (Iv) Carboxylate Complexes / Kelly Tan Ching Yi, Kelly Ching Yi Tan

Student Works (2010-2019)

Two organotin (IV) carboxylate complexes were prepared by the refluxed synthesized in ethanol and acetonitrile respectively. Both reactions are carried out with organotin (IV) complexes and carboxylic acid in 1:1 stoichiometric reaction. The water molecules formed during the reaction is removed from the mixture by molecule sieve and a Dean and Stark apparatus. Complexes have been characterized by elemental analysis, melting point determination, Fourier Transform Infrared spectroscopy and multinuclear Magnetic Resonance (1H, 13C and 119Sn) studies were carried out to elucidate their structures. The differences [υ(COO)], where [υ(COO)as -[υ(COO)s] was determined indicating complex 1 is bidentate with ligand while complex …

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Development Of A Climate Change Vulnerability Index For Peninsular Malaysia / Wong Foong Mei, Foong Mei Wong Jan 2014

Development Of A Climate Change Vulnerability Index For Peninsular Malaysia / Wong Foong Mei, Foong Mei Wong

Student Works (2010-2019)

There are two factors that can affect changes in climate; internal variation and external forcing. The warming and cooling trend is determined by increases of the concentration of greenhouse gases, which consists of water vapour, carbon dioxide, methane and nitrous oxide. Even if greenhouse gas emissions were stabilised instantly at today’s level, the climate would still continue to change as it adapts to the increased emission of recent decades. This is because climate change in the future is greatly influence by the past emissions. Therefore, further changes in climate are unavoidable. Since 21st century, the issue of climate change has …

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Antibacterial Activities Of Selected Marine Fungi From Peninsular Malaysia / Audra Shaleena A/P Paliany, Audra Shaleena Paliany Jan 2014

Antibacterial Activities Of Selected Marine Fungi From Peninsular Malaysia / Audra Shaleena A/P Paliany, Audra Shaleena Paliany

Student Works (2010-2019)

Over the past few decades, scientists have come to acknowledge the growing importance of marine and marine-related resources for new drugs, pesticides and anti-fouling substances, with over 10,000 metabolites isolated from marine organisms to date. In an effort to tap into the pharmaceutical potential of marine fungi in Malaysia, selected marine derived endophytic and manglicolous fungi from the coastlines of Peninsular Malaysia were investigated for their antibacterial potential. The endophytic fungi in this study were isolated from two marine associated plants; Vitex rotundifolia L.f. and Ipomoea pes-caprae (L.) R.Br., from the east and west coast of Peninsular Malaysia respectively. A …

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Volatile Constituents And Trace Metal Analysis Of Alpinia Conchigera Griff. / Haslinda Mohd Salleh, Haslinda Mohd Salleh Jan 2014

Volatile Constituents And Trace Metal Analysis Of Alpinia Conchigera Griff. / Haslinda Mohd Salleh, Haslinda Mohd Salleh

Student Works (2010-2019)

The rhizomes and leaves of Alpinia conchigera were extracted by supercritical fluid extraction (SFE) technique using liquid carbon dioxide at constant temperature (40°C) with two different pressures (A1: leaves extract at 1500 psi; A2: leaves extract at 5000 psi; B1: rhizomes extract at 1500 psi; B2: rhizomes extract at 5000 psi). The volatile constituents of the extracts were detected by GCMS. The major compound was identified as acetoxychavicol acetate in all the extracts. Besides that, β- bisabolene, chavicol, trans-pcoumaryl diacetate and acetoxyeugenol acetate were detected too. The concentration of macronutrients and trace metal for rhizomes and leaves of Alpinia conchigera …

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Organic Petrological And Organic Geochemical Of Tertiary Coals Within The West Middle Block Of The Pinangah Coal Field, Sabah, Malaysia / Fatin Liyana Bt Alias, Fatin Liyana Alias Jan 2014

Organic Petrological And Organic Geochemical Of Tertiary Coals Within The West Middle Block Of The Pinangah Coal Field, Sabah, Malaysia / Fatin Liyana Bt Alias, Fatin Liyana Alias

Student Works (2010-2019)

The Tertiary Tanjong Formation coals exposed in the west middle block of the Pinangah Coalfield, central part of southern Sabah were analysed based on organic petrological and organic geochemical methods. The sediments of the Tanjong Formation were deposited in brackish water condition, based on limited biostratigraphic data (Collenette, 1965). The Tertiary Tanjong coals are humic and generally dominated by vitrinite, with significant amounts of liptinite and low amounts of inertinite macerals. Total organic carbon contents (TOC) of the coals range from 51.2 to 77.7 wt. %, and yield of bitumen values ranging from 57300 to 140000 ppm, which meet the …

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A Study Of Neutrophil Dysfunction Related Disorder In Malaysia: A Case Study On A X-Linked Chronic Granulomatous Disease In A Male Child / Hemahwathy D/O Chanthira Kumar, Hemahwathy Chanthira Kumar Jan 2014

A Study Of Neutrophil Dysfunction Related Disorder In Malaysia: A Case Study On A X-Linked Chronic Granulomatous Disease In A Male Child / Hemahwathy D/O Chanthira Kumar, Hemahwathy Chanthira Kumar

Student Works (2010-2019)

Chronic Granulomatous Disease (CGD) is a primary immunodeficiency disease (PID) in which the defect lies at the neutrophil intracellular killing step of the phagocytosis process. It is caused by a dysfunctional respiratory burst enzyme Nicotinamide Adenine Dinucleotide Phosphate (NADPH)-oxidase, a multi-protein complex making CGD a heterogeneous disease. X-linked CGD (X-CGD) is due to a mutation in the CYBB gene that codes for gp91-phox, while autosomal CGD is caused by mutations in genes that code for p22-phox, p47-phox, p67-phox, p40-phox and Rac2. The incidence of Klinefelter’s Syndrome and CGD occurring together is extremely rare. This study describes a family where the …

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Production And Loss Rates Of Picocyanobacteria In Tropical Coastal Waters / Heng Pei Li, Pei Li Heng Jan 2014

Production And Loss Rates Of Picocyanobacteria In Tropical Coastal Waters / Heng Pei Li, Pei Li Heng

Student Works (2010-2019)

We studied temporal variation of picocyanobacteria and their production (μ) and loss (g) rates for over two years of period at Port Klang (PK) estuarine waters [03º00.1'N, 101º23.4'E] and Port Dickson (PD) coastal waters [02º29.5'N, 101º50.3'E] along the Straits of Malacca. PK waters showed higher level of eutrophication, lower euphotic depth and higher TSS level as compared to PD. Heterotrophic bacterial abundance at PK (2.78 ± 1.58 × 106 cell ml–1) was generally higher than PD (1.39 ± 0.49 × 106 cell ml–1) (Student’s t-test: t = – 5.30, df = 47, p < 0.001). In contrast, picocyanobacterial abundance at PD (1.33 ± 0.47 × 105 cell ml–1) was always higher than at PK (0.28 ± 0.17 × 105 cell ml–1) (Student’s t-test: t = 10.44, df = 30, p < 0.001). μ and g of picocyanobacteria were tightly coupled with each other (R2 = 0.47, df = 459, p < 0.001) and similar at both sites (p > 0.05). μ ranged from – 0.03 to …

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Molecular And Functional Indication Of Chalcone Synthase In Boesenbergia Rotunda / Fatemeh Shahhosseini, Fatemeh Shahhosseini Jan 2014

Molecular And Functional Indication Of Chalcone Synthase In Boesenbergia Rotunda / Fatemeh Shahhosseini, Fatemeh Shahhosseini

Student Works (2010-2019)

Chalcone synthase (CHS) is a key enzyme in flavonoids biosynthesis pathway, which catalyzes the condensation of three acetate residues from malonyl-CoA with pcoumaroyl- CoA to form naringenin chalcone. This step is the first committed step of the phenylpropanoid pathway, which regulates other sub-branches to produce flavonoids, isoflavonoids, anthocyanin, chalcone, and other flavonoids compounds in plants. Several studies have shown that the flavonoids and chalcones are pharmaceutically active in Boesenbergia rotunda, but the purification is often impossible due to the low concentration of these flavonoids. Most studies recently focused on the individual genes of the pathway such as CHS gene to …

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Quantification Of Arsenic In Soil By Inductively Coupled Plasma – Optical Emission Spectrometry / Vickneswary A/P Letchmanan, Vickneswary Letchmanan Jan 2014

Quantification Of Arsenic In Soil By Inductively Coupled Plasma – Optical Emission Spectrometry / Vickneswary A/P Letchmanan, Vickneswary Letchmanan

Student Works (2010-2019)

In this study, determination of arsenic in soil samples was done by Inductively Coupled Plasma – Optical Emission Spectrometry. Soil from five different locations with three sampling points was collected. The location chosen were garden, dam, beach, construction and agriculture areas. A standard method was used for the sample preparation using HotBlockTM system. The highest arsenic concentrated found in the construction area (32.24 ± 0.02 mg/kg) and followed by agriculture (31.55 ± 0.03 mg/kg), garden (19.17 ± 0.02 mg/kg), dam (17.12 ± 0.01 mg/kg) and beach (1.73 ± 0.01 mg/kg).

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Recovery Of Lignin Peroxidase From Amauroderma Rugosum Using Aqueous Two-Phase System / Zeinabsadat Masoudian, Zeinabsadat Masoudian Jan 2014

Recovery Of Lignin Peroxidase From Amauroderma Rugosum Using Aqueous Two-Phase System / Zeinabsadat Masoudian, Zeinabsadat Masoudian

Student Works (2010-2019)

A very important challenge in industrial biotechnology is development of an efficient downstream method for enzyme purification and separation due to the growing market of enzyme in biotechnology. Within the scope of this study, aqueous two-phase system based on imidazolium ionic liquid as an efficient downstream processing method was evaluated for primary purification of lignin peroxidase. The white-rot fungi Amauroderma rugosum is capable to produce lignin peroxidase as one of the ligninolytic enzymes. Lignin peroxidase (LiP) is an extracellular hem protein enzyme which is able to degrade lignin or lignin related aromatic compounds. The fungus cultivation in liquid media comprised …

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Quantification And Bioremediation Of Landfill Methane / Siva Shangari A/P Govindan, Siva Shangari Govindan Jan 2014

Quantification And Bioremediation Of Landfill Methane / Siva Shangari A/P Govindan, Siva Shangari Govindan

Student Works (2010-2019)

Methane (CH4) a potent greenhouse gas (GHG), has more than doubled in past decades and continues to rise. Historically, landfills have been the largest source of anthropogenic CH4 emission from the waste sector with 6,875 million metric tonnes of CO2–eq in year 2010. Therefore, it is important to quantify CH4 emissions from landfills to evaluate measures for reduction of GHG emission which contributes to global warming and climate change. At present, CH4 emission rates from landfills have been estimated using mathematical and empirical models and among them, the first-order-decay (FOD) models are often credited for its estimation. The objective of …

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Microwave Synthesis Of Zno/Rgo Nanocomposites For Enhanced Degradation Of Dye / Fatin Saiha Binti Omar, Fatin Saiha Omar Jan 2014

Microwave Synthesis Of Zno/Rgo Nanocomposites For Enhanced Degradation Of Dye / Fatin Saiha Binti Omar, Fatin Saiha Omar

Student Works (2010-2019)

Zinc oxide/reduced graphene oxide (ZnO/rGO) nanocomposites were successfully synthesized in the presence of diethylenetriamine (DETA) via a facile microwave assisted method. The x-ray diffraction (XRD) patterns of the ZnO/rGO nanocomposites reveal that obtained nanocomposite materials containing ZnO in hexagonal phase with wurtzite structure. The high-resolution transmission electron microscopy (HRTEM) images indicates that the prepared nanocomposites having ZnO nanorods, with an average length:diameter ratio of 10 and which is found to be deposited onto the rGO sheets. Under the irradiation of sunlight, the ZnO/rGO nanocomposites showed two-fold improved photocatalytic performance than that of unmodified ZnO towards the photodegradation of methylene blue. …

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Identification Of A Novel Invariant Splice Site Mutation Of Bruton's Tyrosine Kinase (Btk) Gene In A Malaysian Family With X-Linked Agammaglobulinemia / Chear Chai Teng, Chai Teng Chear Jan 2014

Identification Of A Novel Invariant Splice Site Mutation Of Bruton's Tyrosine Kinase (Btk) Gene In A Malaysian Family With X-Linked Agammaglobulinemia / Chear Chai Teng, Chai Teng Chear

Student Works (2010-2019)

X-linked agammaglobulinemia (XLA) is a rare genetic disorder caused by mutations in the Bruton’s tyrosine kinase (BTK) gene. These mutations cause defects in early B cell development. A patient with no circulating B cells and low serum immunoglobulin isotypes was studied as were his mother and sister. Flow cytometry showed the patient lacked BTK protein expression in his monocytes while the mother and sister had 62% and 40% of the monocytes showing BTK protein expressions, respectively. Results from genetic studies revealed that the patient had a novel base substitution in the first nucleotide of intron 9 in the BTK gene, …

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An Improved Method For The Stilbenes Synthesis Via The Heck Reaction And Approach To Indole Synthesis Using Pida / Ang Kheng Ping, Kheng Ping Ang Jan 2014

An Improved Method For The Stilbenes Synthesis Via The Heck Reaction And Approach To Indole Synthesis Using Pida / Ang Kheng Ping, Kheng Ping Ang

Student Works (2010-2019)

Six iodoanilides incorporating different amide functionalities (e.g butyramide, furan carboxamide, cyclohexylamide, (2.1 to 2.6)) were prepared by established methods. These iodocarboxamides were transformed to the corresponding amido stilbenes (2.5 to 2.12) by an improved Heck procedure. Following silica gel chromatography, the resulting stilbene orthocarboxamides were exposed to diacetoxy iodobenzene with a view to indole synthesis.

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Development Of A Decision Support System (Dss) For Malaysian Adult Weight Management / Aadilah Binti Baharuddin, Aadilah Baharuddin Jan 2014

Development Of A Decision Support System (Dss) For Malaysian Adult Weight Management / Aadilah Binti Baharuddin, Aadilah Baharuddin

Student Works (2010-2019)

The main objective of this study is to develop an electronic knowledge-based decision support system (DSS) for the use of experts, specifically nutritionists and dieticians, as well as Malaysian adults for effective weight management. The system will allow the user to keep track of their daily food consumption and energy expenditure to prevent them from getting overweight since obesity is a prominent public health problem in Malaysia. It is also in line with goals of the National Plan of Action for Nutrition of Malaysia (NPANM) for the period of 2006 to 2015 to reduce overweight individuals and prevent obesity among …

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