Digital Commons Network^™

Using Phylogenetically-Informed Annotation (Pia) To Search For Light-Interacting Genes In Transcriptomes From Non-Model Organisms, Daniel L. Speiser, Molly S. Pankey, Alexander K. Zaharoff, Barbara A. Battelle, Heather D. Bracken-Grissom, Jesse W. Breinholt, Seth M. Bybee, Thomas W. Cronin, Anders Garm, Annie R. Lindgren, Nipam H. Patel, Megan L. Porter, Meredith E. Protas, Ajna S. Rivera, Jeanne M. Serb, Kirk S. Zigler, Keith A. Crandall, Todd H. Oakley

Biology Faculty Publications and Presentations

Background: Tools for high throughput sequencing and de novo assembly make the analysis of transcriptomes (i.e. the suite of genes expressed in a tissue) feasible for almost any organism. Yet a challenge for biologists is that it can be difficult to assign identities to gene sequences, especially from non-model organisms. Phylogenetic analyses are one useful method for assigning identities to these sequences, but such methods tend to be time-consuming because of the need to re-calculate trees for every gene of interest and each time a new data set is analyzed. In response, we employed existing tools for phylogenetic analysis to …

In Vivo Identification Of Eugenol-Responsive And Muscone-Responsive Mouse Odorant Receptors, Timothy S. Mcclintock, Kaylin Adipietro, William B. Titlow, Patrick Breheny, Andreas Walz, Peter Mombaerts, Hiroaki Matsunami

Physiology Faculty Publications

Our understanding of mammalian olfactory coding has been impeded by the paucity of information about the odorant receptors (ORs) that respond to a given odorant ligand in awake, freely behaving animals. Identifying the ORs that respond in vivo to a given odorant ligand from among the ∼1100 ORs in mice is intrinsically challenging but critical for our understanding of olfactory coding at the periphery. Here, we report an in vivo assay that is based on a novel gene-targeted mouse strain, S100a5-tauGFP, in which a fluorescent reporter selectively marks olfactory sensory neurons that have been activated recently in vivo. Because each …

The Bg News November 19, 2014, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. November 19, 2014. Volume 94-Issue 38.

Frequency And Fitness Consequences Of Bacteriophage Φ6 Host Range Mutations, Brian E. Ford, Bruce Sun, James Carpino, Elizabeth S. Chapler, Jane Ching, Yoon Choi, Kevin Jhun, Jung D. Kim, Gregory G. Lallos, Rachelle Morgenstern, Shalini Singh, Sai Theja, John J. Dennehy

Publications and Research

Viruses readily mutate and gain the ability to infect novel hosts, but few data are available regarding the number of possible host range-expanding mutations allowing infection of any given novel host, and the fitness consequences of these mutations on original and novel hosts. To gain insight into the process of host range expansion, we isolated and sequenced 69 independent mutants of the dsRNA bacteriophage Φ6 able to infect the novel host, Pseudomonas pseudoalcaligenes. In total, we found at least 17 unique suites of mutations among these 69 mutants. We assayed fitness for 13 of 17 mutant genotypes on P. pseudoalcaligenes …

Host, Pathogen, And Environmental Characteristics Predict White-Nose Syndrome Mortality In Captive Little Brown Myotis (Myotis Lucifugus), Joseph S. Johnson, Deeann M. Reeder, James W. Mcmichael Iii

KIP Articles

An estimated 5.7 million or more bats died in North America between 2006 and 2012 due to infection with the fungus Pseudogymnoascus destructans (Pd) that causes white-nose syndrome (WNS) during hibernation. The behavioral and physiological changes associated with hibernation leave bats vulnerable to WNS, but the persistence of bats within the contaminated regions of North America suggests that survival might vary predictably among individuals or in relation to environmental conditions. To investigate variables influencing WNS mortality, we conducted a captive study of 147 little brown myotis (Myotis lucifugus) inoculated with 0, 500, 5 000, 50 000, or 500 000 Pd …

Future Research And Clinical Directions In The Field Of Men's Mental Health: The Madrid Declaration, Leo Sher, Zoltan Rihmer, Javier Didia-Attas, Jose De Leon, Carlos Roncero, Nestor Szerman

Psychiatry Faculty Publications

The members of the World Federation of Biological Psychiatry’s Task Force on Men’s Mental Health met in Madrid in September 2014 to discuss the research and clinical directions in the field of Men’s Mental Health. Leo Sher, M.D. (USA), Zoltan Rihmer, M.D., Ph.D. (Hungary), Javier Didia-Attas, M.D. (Argentina), Jose de Leon, M.D. (USA), Shih-Ku Lin, M.D. (Taiwan), Carlos Roncero, M.D. (Spain), and Nestor Szerman, M.D. (Spain) participated in the meeting. The following consensus recommendations were made.

Glucose Significantly Enhances Enterotoxigenic Escherichia Coli Adherence To Intestinal Epithelial Cells Through Its Effects On Heat-Labile Enterotoxin Production, Prageeth Wijemanne, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

The present study tested whether exposure of enterotoxigenic Escherichia coli (ETEC) to glucose at different concentrations in the media results in increased bacterial adherence to host cells through increased heat-labile enterotoxin (LT) production, thereby suggesting the effects are physiological. Porcine-origin ETEC strains grown in Casamino acid yeast extract medium containing different concentrations of glucose were washed and inoculated onto IPEC-J2 porcine intestinal epithelial cells to test for effects on adherence and host cell cAMP concentrations. Consistent with previous studies, all LT+ strains had higher ETEC adherence to IPEC-J2 cells than did LT2 strains. Adherence of the LT2 but not the …

The Winonan, Winona State University

The Winonan - 2010s

No abstract provided.

Metallopeptides From Design To Catalysis: Structure, Oxidative Activities, And Inhibition Studies Of Designed And Naturally Occurring Metallopeptides, Alaa Hassan Hashim

USF Tampa Graduate Theses and Dissertations

Structural and mechanistic complexities of copper-dioxygen systems have attracted much attention in the field of bioinorganic chemistry, both in model systems and trapped protein intermediates. The research presented herein is focused on model and naturally occurring metallopeptide systems, from its design to catalysis. Copper is used as the coordinating metal ion, with cobalt and zinc as probes for metal binding. The bioinorganic chemistry of copper proteins and its coordination and spectroscopic properties are briefly discussed in chapter 1. The next two chapters are centered on the de novo design of a minimalistic metallopeptide system with an amino acid sequence of …

Co(Ii) Based Metalloradical Catalysis: Carbene And Nitrene Transfer Reactions, Joseph B. Gill

USF Tampa Graduate Theses and Dissertations

Radical chemistry has attracted a large amount of research interest over the last few decades and radical reactions have recently been recognized as powerful tools for organic synthesis. The synthetic applications of radicals have been demonstrated in many fields, including in the synthesis of complex natural products. Radical reactions have a number of inherent synthetic advantages over their ionic counterparts. For example, they typically proceed at fast reaction rates under mild and neutral conditions in a broad spectrum of solvents and show significantly greater functional group tolerance. Furthermore, radical processes have the capability of performing in a cascade fashion, allowing …

Climate Change Response At The Farm Level: A Review Of Farmers’ Awareness And Adaptation Strategies In Developing Countries, Nichola Harmer, Sanzidur Rahman

School of Geography, Earth and Environmental Sciences

This paper provides an overview of farmers’ awareness and a typology of adaptation strategies undertaken in order to combat climate change at the farm-level in developing countries. The review suggests that the extent to which farmers are aware of climate change is uneven, and the link between perception and action is often unclear. Farmers adopt a range of strategies from financial responses, to agricultural changes, to religious and cultural strategies, to the use of local and wider support networks. However, whether these actions are adaptation or coping strategies is debatable. Response to climate change has both spatial and temporal dimensions; …

Patient Safety Culture, Compassion Fatigue, Compassion Satisfaction: Impact On Nurse-Sensitive Patient Outcomes, Debbie Anglade

Open Access Dissertations

Improved patient safety has been one of the most critical issues facing the healthcare industry. The 1999 IOM report To Err Is Human stated avoidable medical errors contributed to 44,000 to 98,000 deaths in the United States. Current estimates are now between 210,000 and 440,000 patients annually. This study aimed: To examine the relationships between: a) inpatient nursing units’ patient safety culture (PSC), b) nurse compassion fatigue (CF), c) nurse compassion satisfaction (CS), and d) the impact on nurse-sensitive patient outcomes. Bronfenbrenner’s bioecological model guided this quantitative descriptive correlational design, which used primary and secondary data. Primary data were collected …

Inside The Uconn Libraries, Volume 1, Number 1

UConn Libraries Newsletter

page 1 J.W. "Jack" Swanberg

On Track with an Eminent Railroad Historian, Collector, and Donor

page 2 Message from the Vice Provost Martha Bedard

page 3 Professor Emeritus Rudy J. Favretti

Noted Historic Landscape Architect Has Sights on Mansfield's Past

page 4 Stanley L. Nash

Professor Emeritus Honors Libraries with Gift of Books on Hebrew and Israeli Literature

page 6 Events: Northeast Children's Literature Collection Reception

page 7 Winter Art Exhibits

From Transylvania to Tolland: the Art of Liviu Cupceancu

Current Illustration and Photography from UConn's Faculty in Art and Art History

Illustration - Cora Lynn Deibler, Alison Paul

Photography …

The Patriot Talon (November 18, 2014), University Of Texas At Tyler

The Patriot Talon/The Patriot

The official newspaper for the University of Texas at Tyler before it was changed back to the UT Tyler Patriot. Articles in this issue include: Construction projects right on schedule, Expansions include music building addition, biology labs and pharmacy school; Is it nap time yet? Sleep deprivation affects health and learning; Pi Kappa Alpha coming to University; Greek students delete Yik Yak in protest of bullying; Religion does not excuse crime; Society and economy are factors in obesity; Being alone can be an essential part of growing up; Revamping the Community, Local church motivates Tyler community to donate canned food; …

Flexible And Scalable Genotyping-By-Sequencing Strategies For Population Studies, Christopher Heffelfinger, Christopher A. Fragoso, Maria A. Moreno, John D. Overton, John P. Mottinger, Hongyu Zhao, Joe Tohme, Stephen L. Dellaporta

Cell and Molecular Biology Faculty Publications

Background: Many areas critical to agricultural production and research, such as the breeding and trait mapping in plants and livestock, require robust and scalable genotyping platforms. Genotyping-by-sequencing (GBS) is a one such method highly suited to non-human organisms. In the GBS protocol, genomic DNA is fractionated via restriction digest, then reduced representation is achieved through size selection. Since many restriction sites are conserved across a species, the sequenced portion of the genome is highly consistent within a population. This makes the GBS protocol highly suited for experiments that require surveying large numbers of markers within a population, such as those …

Spartan Daily, November 18, 2014, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily (School of Journalism and Mass Communications)

Volume 143, Issue 33

2014 November 18 - Medical Student Education Committee Meeting Minutes, Medical Student Education Committee, East Tennessee State University

Medical Student Education Committee Minutes

No abstract provided.

Global Leader In Water Treatment Industry Opens Cache Valley Office And New Partnership With College Of Engineering, Usu College Of Engineering

College of Engineering News

An international leader in the water and wastewater industry is opening a new office in Cache Valley to take advantage of what the company calls an ideal location and a talented workforce of new engineering graduates.

Biology Alum Named Supervising Pharmacist At New Store, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

The Tiger Vol. 108 Issue 39 2014-11-18, Clemson University

Tiger Newspapers

No abstract provided.

Bringing The U. To You, Tracey Bentley

UNH Today Archive

No abstract provided.

The Current Volume 25 : Issue 13, Nova Southeastern University

Student Newspapers

No abstract provided.

Parents' And Children's Emotions Spanning The Hiv Disclosure Process In Kenya, Grace Gachanja, Gary J. Burkholder Jr, Aimee Ferraro

Walden Faculty and Staff Publications

2014 APHA Annual Conference Tuesday, November 18, 2014 New Orleans, LA

HIV disclosure from parent to child is challenging. While disclosure is expected to be emotion for parents and children, the total disclosure experience has not been described. The purpose of this study was to understand the lived experiences of HIV - positive parents and their children in Kenya during the disclosure process.

11-18-2014 Wildlife Biologist To Talk Wednesday At Swosu, Southwestern Oklahoma State University

THE BARK ARCHIVE 2014

Levi Feltman, wildlife biologist at the Washita National Wildlife Refuge, will speak at 4 p.m. this Wednesday, November 19, on the Southwestern Oklahoma State University campus.

Daily Eastern News: November 18, 2014, Eastern Illinois University

November

No abstract provided.

A Raman Microspectroscopy Study Of Water And Trehalose In Spin-Dried Cells, Alireza Abazari, Nilay Chakraborty, Steven Hand, Alptekin Aksan, Mehmet Toner

Faculty Publications

© 2014 Biophysical Society. Long-term storage of desiccated nucleated mammalian cells at ambient temperature may be accomplished in a stable glassy state, which can be achieved by removal of water from the biological sample in the presence of glass-forming agents including trehalose. The stability of the glass may be compromised due to a nonuniform distribution of residual water and trehalose within and around the desiccated cells. Thus, quantification of water and trehalose contents at the single-cell level is critical for predicting the glass formation and stability for dry storage. Using Raman microspectroscopy, we estimated the trehalose and residual water contents …

The Formin Fmnl3 Assembles Plasma Membrane Protrusions That Participate In Cell–Cell Adhesion, Timothy J. Gauvin, Lorna E. Young, Henry N. Higgs

Dartmouth Scholarship

FMNL3 is a vertebrate-specific formin protein previously shown to play a role in angiogenesis and cell migration. Here we define the cellular localization of endogenous FMNL3, the dynamics of GFP-tagged FMNL3 during cell migration, and the effects of FMNL3 suppression in mammalian culture cells. The majority of FMNL3 localizes in a punctate pattern, with >95% of these puncta being indistinguishable from the plasma membrane by fluorescence microscopy. A small number of dynamic cytoplasmic FMNL3 patches also exist, which enrich near cell–cell contact sites and fuse with the plasma membrane at these sites. These cytoplasmic puncta appear to be part of …

The George-Anne, Georgia Southern University

The George-Anne

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Cosm News, Georgia Southern University

College of Science & Mathematics: News & Publications (2012-2022)

• Biology Student & Faculty featured in GS Foundation Annual Report

The Parthenon, November 18, 2014, Codi Mohr

The Parthenon

The Parthenon, Marshall University’s student newspaper, is published by students Monday through Friday during the regular semester and weekly Thursday during the summer. The editorial staff is responsible for the news and the editorial content.