Digital Commons Network^™

Evaluation Of The Physiological Effects Of Reduced Hydroxocobalamin On Acute Carbon Monoxide Toxicity, Alden Newcomb

Theses and Dissertations

Carbon monoxide (CO) poisoning represents a global health threat responsible for hundreds of thousands of hospital visits and tens of thousands of deaths annually. Oxygen therapy is the only current approved treatment for CO poisoning. Previous work published in the 1970’s and research conducted in the VCURES lab group has indicated that a reduced form of vitamin B12, hydroxocobalamin (B12r), can potentially serve as an antidote for CO poisoning by converting CO bound to hemoglobin to carbon dioxide (CO2) and mitigating the deleterious neurological effects of CO poisoning. For the first time in documented literature we successfully used a Clark-type …

Regulation And Mechanistic Functions Of Caspase-9 Rna Splicing, Ngoc T. Vu

Theses and Dissertations

Caspase-9 has two splice variants, pro-apoptotic caspase-9a and anti-apoptotic caspase-9b, and dysregulation of caspase-9 splice variant ratio or expression of caspase-9b isoform has been linked to augmentation of the anchorage-independent growth and tumorigenic capacity of non-small cell lung cancer (NSCLC) cells. This study focuses on cell signaling pathway(s) regulating the alternative splicing of caspase-9 pre-mRNA and mechanistic roles of caspase-9b in a certain oncogenic/survival pathway. In regards to the former, we have identified hnRNP U as a novel splice-enhancer associated with exon 3 of caspase-9 (C9/E3). Moreover, hnRNP U binds specifically to C9/E3 at an RNA cis-element previously reported …

The Effect Of Fluvastatin On Mast Cell Function: Genotype Dependence, Elizabeth M. Kolawole

Theses and Dissertations

Fluvastatin, the HMG-CoA reductase inhibitor known for its role in the treatment of hypercholesterolemia and cardiovascular disease, has more recently been shown to play a role in the immune response. Given the critical role that mast cells play in allergy and inflammatory diseases such as asthma, which effects one third of America’s population, we assessed the effect of fluvastatin on mast cell and basophils function. We demonstrate that fluvastatin downregulated IgE-mediated cytokine production. Additionally, in vivo studies showed that fluvastatin suppressed IgE-mediated anaphylaxis. Interestingly, the effects of fluvastatin showed dependence on genetic background, as C57BL/6 mast cells were sensitive, while …

Quantitative Mass Spectrometric Investigations Of Protein Biomarkers: Serum Thymidine Kinase 1 And Human Osteopontin, Morse Faria

Theses and Dissertations

Mass spectrometry is being increasingly used in biomarker research mainly due to its ability to achieve high selectivity coupled with high sensitivity. This dissertation focuses on quantitative mass spectrometric investigations of two protein biomarkers i.e. serum thymidine kinase 1 (TK1) and human osteopontin (OPN).

First part of this research was focused on developing a liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method for measuring the activity of TK1 in serum by monitoring the conversion of a TK1 specific exogenous substrate, 3’-deoxy-3’-fluorothymidine (FLT), to its mono-phosphorylated form 3’-deoxy-3’-fluorothymidine monophosphate (FLT-MP). A method to quantify FLT-MP on LC-MS/MS was developed and …

A Study Of A Professional Development Initiative To Increase Cultural Competency, Jennifer C. Coleman

Theses and Dissertations

Abstract

A STUDY OF A PROFESSIONAL DEVELOPMENT INITIATIVE TO INCREASE

CULTURAL COMPETENCY

By Jennifer Crowe Coleman, Ph.D.

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University.

Virginia Commonwealth University, 2014

Major Director: Charol Shakeshaft, Ph.D.

Professor, Department of Educational Leadership

School of Education

While there are broad, societal forces that contribute to achievement gaps, the professional development of teachers is one way educational leaders have sought to improve schools, improve teacher performance, and increase teacher knowledge and skills. Given the achievement and cultural gaps that exist between teachers and …

Structural And Functional Characterization Of The Mbd2-Nurd Co-Repressor Complex, Megha Desai

Theses and Dissertations

The MBD2-NuRD co-repressor complex is an epigenetic regulator of the developmental silencing of embryonic and fetal β-type globin genes in adult erythroid cells as well as aberrant methylation-dependent silencing of tumor suppressor genes in neoplastic diseases. Biochemical characterization of the MBD2-NuRD complex in chicken erythroid cells identified RbAp46/48, HDAC1/2, MTA1/2/3, p66α/β, Mi2α/β and MBD2 to comprise this multi-protein complex.

In the work presented in Chapter 2, we have pursued biophysical and molecular studies to describe a previously uncharacterized domain of human MBD2 (MBD2_IDR). Biophysical analyses show that MBD2_IDR is an intrinsically disordered region (IDR). Despite this inherent …

Aquatic Priming Effects In The York River Estuary And Implications For Dissolved Organic Carbon Mineralization, Thomas M. Dunlap

Theses and Dissertations

The priming effect (PE), characterized as the enhanced microbial processing of bio-recalcitrant organic matter with the addition of labile substrates, has been hypothesized to moderate carbon (C) cycling in aquatic systems. In this study, aquatic PEs were evaluated through bacterial respiration and dissolved organic C consumption in incubations of water collected from three locations along the York River estuary. Incubations from White’s Landing on the Pamunkey River, a tidal freshwater tributary of the York, and from Croaker Landing in the middle of the estuary, displayed positive PEs when amended with labile C. In contrast, amended incubations from Gloucester Point, near …

The Effect Of Lactic Acid On Mast Cell Function, Andrew J. Spence

Theses and Dissertations

This study shows for the first time the effect that L-(+)-lactic acid has on mast cell activation. Lactic acid is a byproduct of anaerobic glycolysis and is associated with inflammatory environments such as wounds, tumors and, asthma. In this study, pre-treatment with lactic acid altered cytokine production by bone marrow-derived mast cells (BMMC). Specifically, lactic acid enhanced cytokine secretion following IgE cross-linking, but decreased IL-33 mediated cytokine production. These effects were altered by genetic background, since C57BL/6 mast cells demonstrated the aforementioned result, but lactic acid had no effect on IgE-mediated cytokine production in 129/SvJ mast cells. The affected cytokines …

3d Reconstruction Of Ryr1 And Structural Validation Under Different Levels Of Noise, Joshua J. Lobo

Theses and Dissertations

Ryanodine receptors (RyR) are intracellular channels that are intricately involved in Ca2+ release. These channels large membrane proteins~2.26MDa in size. In this multi-goal project firstly we successfully studied the gating mechanics of the RyR1 in the presence of Mg2+. We used single particle reconstruction and image processing techniques to obtain the 3D structure of the RyR1 with Mg2+. The 3D structure in the presence of Mg2+ and an ATP analog is the closest representation of human physiological conditions. The open and closed state structures of RyR1 are known. However, the physiologically closed state has not been studied before. Understanding this …

Family Needs, Caregiver Burden, And Mental Health: Caregivers Of Individuals With Various Neurological Conditions From Colombia And Mexico, Megan Elizabeth Sutter

Theses and Dissertations

This cross-sectional study examined differences in family needs (informational, social, financial, health, and household support), caregiver mental health (depression, satisfaction with life, vitality, social functioning, and emotional role limitations), and caregiver burden (personal life, guilt, and psychological) among caregivers of individuals with traumatic brain injury, spinal cord injury, multiple sclerosis, and dementia from cities in Colombia and Mexico (N = 343). The study also examined the connections among family needs, caregiver mental health, and caregiver burden in the combined sample of caregivers of individuals with neurological conditions. Many significant differences were identified among groups, and implications are discussed. Family …

Exploring The Asymmetric Environment Of Various Chiral Catalysts Using A Modified Ion-Trap Mass Spectrometer: Towards The Development Of A Rapid Chiral Catalyst Screening Method, Cary M. Davis

Theses and Dissertations

Since the tragedy of the drug Thalidomide® in the late 1950 to early 1960’s, chirality has been recognized as an important aspect that must be controlled in the drug development process in the pharmaceutical industry. Since then, there has been a considerable movement towards single enantiomer drugs. This demand has presented many challenges for the synthetic organic chemist. Chiral catalysts offer one solution to this problem, as they afford the unique ability to preferentially synthesize one enantiomer. Unfortunately, the design of new chiral catalysts is often empirical, with luck and trial and error necessary due to factors that govern enantioselectivity. …

Analysis And Modeling Of The Roles Of Actin-Myosin Interactions In Bladder Smooth Muscle Biomechanics, Seyed Omid Komariza

Theses and Dissertations

Muscle mechanical behavior potentially plays an important role in some of the most common bladder disorders. These include overactive bladder, which can involve involuntary contractions during bladder filling, and impaired contractility or underactive bladder, which may involve weak or incomplete contractions during voiding. Actin-myosin cross-bridges in detrusor smooth muscle (DSM) are responsible for contracting and emptying the bladder. The total tension produced by muscle is the sum of its preload and active tensions. Studies suggest that actin-myosin cross-links are involved in adjustable preload stiffness (APS), which is characterized by a preload tension curve that can be shifted along the length …

Statistical Modeling Of Interfractional Tissue Deformation And Its Application In Radiation Therapy Planning, Douglas J. Vile

Theses and Dissertations

In radiation therapy, interfraction organ motion introduces a level of geometric uncertainty into the planning process. Plans, which are typically based upon a single instance of anatomy, must be robust against daily anatomical variations. For this problem, a model of the magnitude, direction, and likelihood of deformation is useful. In this thesis, principal component analysis (PCA) is used to statistically model the 3D organ motion for 19 prostate cancer patients, each with 8-13 fractional computed tomography (CT) images. Deformable image registration and the resultant displacement vector fields (DVFs) are used to quantify the interfraction systematic and random motion. By applying …

Language Skills In Incarcerated Young Offenders: Links With Offending Severity, Pamela Snow

Language as a Social Justice Issue Conference

Oral language competence (i.e. skills with verbal expression and auditory comprehension) underpins and emerges out of, early social-emotional relationships, and forms the basis of the transition to literacy in early childhood. Some outcomes of suboptimal language development in the early years include difficulties forming and maintaining interpersonal relationships, and identifying and understanding affective states of others. This paper presents findings from a study of 100 incarcerated young male offenders in Victoria, Australia. We sought to determine the prevalence of language impairment in this very high-risk group, and also examined correlates of language impairment that could act as early intervention levers, …

Securing Identity Information With Image Watermarks, Brian Cusack, Reza Khaleghparas

Australian Information Security Management Conference

In this paper, we describe the requirements for embedding watermarks in images used for identity verification and demonstrate a proof of concept in security sciences. The watermarking application is designed for verifying the rightful ownership of a driving license or similar identity object. The tool we built and tested embeds and extracts watermarks that contain verification information of the rightful owner. We used the human finger print of the rightful owner as the watermark. Such information protection mechanisms add an extra layer of security to the information system and improve verification of identification attributes by providing strong security. The issues …

Phenological Changes In The Chemical Content Of Wild And Greenhouse-Grown Hypericum Pruinatum: Flavonoids, Cüneyt Çirak, Jolita Radusiene, Liudas Ivanauskas, Valdas Jakstas, Necdet Çamaş

Turkish Journal of Agriculture and Forestry

The present study was conducted to determine the phenological changes in the content of main flavonoids, namely amentoflavone, hyperoside, isoquercitrin, quercitrin, quercetin, avicularin, rutin, (+)-catechin, and (-)-epicatechin in different tissues of Hypericum pruinatum, a promising medicinal herb of the Turkish flora. The wild-growing and greenhouse-grown plants were harvested at vegetative, floral budding, full flowering, fresh fruiting, and mature fruiting stages and dissected into stem, leaf, and reproductive tissues, which were dried separately and subsequently assayed for flavonoid contents by high performance liquid chromatography (HPLC). Chemical contents in whole plants increased during plant phenology and were similar quantitatively for both wild …

Efficient In Vitro Plant Regeneration From Immature Embryos Of Endemic Iris Sari And I. Schachtii, Sati Uzun, Ali̇ İrfan İlbaş, Ari̇f İpek, Neşet Arslan, Surendra Barpete

Turkish Journal of Agriculture and Forestry

Plant tissue culture is an efficient technique for conserving endemic plant species. A reproducible in vitro regeneration protocol was developed for the endemic Iris sari and Iris schachtii in the present study. The highest number of shoots per explant was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L thidiazuron (TDZ) plus 0.5 mg/L α-naphthaleneacetic acid (NAA) and 1 mg/L TDZ plus 0.5 mg/L NAA, whereby 96.88% and 100% shoot induction with 9.55 and 11.34 shoots per explant of Iris sari and Iris schachtii were recorded, respectively. Regenerated shoots were successfully rooted on MS medium with either 1 …

Transcriptomic Analysis Of Tomato Lines Reveals Putative Stress-Specific Biomarkers, Monther Taha Sadder, Abdullah Alsadon, Mahmoud Wahb-Allah

Turkish Journal of Agriculture and Forestry

Different abiotic stresses recruit dedicated signaling and regulatory genes in plants. Genome-wide stress-specific biomarkers were investigated in tomatoes. Three major abiotic stresses were compared: drought, heat, and salinity. For each stress type, 2 different tomato lines were included: susceptible and tolerant. Gene expression was examined by hybridizing to an available tomato microarray. Several stress responsive genes were upregulated in tolerant as well as in susceptible lines for each stress. Comparative analysis of gene expression in response to stress (drought, heat, or salinity) resolved a number of common biomarkers, while other groups of putative biomarkers were associated with each abiotic stress. …

Distribution, Occurrence Of Cry Genes, And Lepidopteran Toxicity Of Native Bacillus Thuringiensis Isolated From Fig Tree Environments In Aydın Province, Mehli̇ka Alper, Hati̇ce Güneş, Arzu Tatlipinar, Beki̇r Çöl, Hasan Sungur Ci̇velek, Cem Özkan, Burcu Poyraz

Turkish Journal of Agriculture and Forestry

Bacillus thuringiensis (Bt) has a significant impact on biological pest control because of the insecticidal activity through its parasporal inclusion bodies (crystal proteins). Fig is an economically important plant in Turkey; agricultural pests result in a considerable economic loss in fig quality and cultivation. The aim of this work was to isolate, characterize, and determine the lepidopteran toxicity of Bt obtained from fig groves in Aydın Province. A total of 606 colonies (out of 1167) obtained from 380 samples were identified as Bt based on parasporal crystal formation. The highest Bt index of 0.60 was observed in the Kuyucak region. …

The Subject Librarian Newsletter, Biology, Spring 2014, Patti Mccall

Libraries' Newsletters

No abstract provided.

Taylor & Francis Archive Collections, Ucf Libraries

Technology Fee Funded Projects

With this purchase, UCF will gain access and perpetual rights to all of the journal content in these four subject collections, starting with the first volume and issue of each journal continuing through 1996. The collection consists of over 400 titles.

Recombinant Interleukin-21 Plus Sorafenib For Metastatic Renal Cell Carcinoma: A Phase 1/2 Study, Shailender Bhatia, Brendan, Marc S. Ernstoff, Michael, Elisabeth I. Heath, Wilson H. Miller Jr, Igor Puzanov, David I. Quinn, Thomas, Peter Vanveldhuizen, Kelly, Jeremy, Rachel, Naomi, Sonia, John A. Thompson

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Despite the positive impact of targeted therapies on metastatic renal cell carcinoma (mRCC), durable responses are infrequent and an unmet need exists for novel therapies with distinct mechanisms of action. We investigated the combination of recombinant Interleukin 21 (IL-21), a cytokine with unique immunostimulatory properties, plus sorafenib, a VEGFR tyrosine kinase inhibitor.

Methods

In this phase 1/2 study, 52 mRCC patients received outpatient treatment with oral sorafenib 400 mg twice daily plus intravenous IL-21 (10–50 mcg/kg) on days 1–5 and 15–19 of each 7-week treatment course. The safety, antitumor activity, pharmacokinetic and pharmacodynamic effects of the combination were …

Are Mothers Hazardous To Their Children’S Health?: Law, Culture, And The Framing Of Risk, Linda C. Fentiman

Elisabeth Haub School of Law Faculty Publications

This Article examines the psychosocial processes of risk construction and explores how these processes intersect with core principles of Anglo-American law. It does so by critiquing current cultural and legal perceptions that mothers, especially pregnant women, pose a risk to their children’s health. The Article’s core argument is that during the last four decades, both American society and American law have increasingly come to view mothers as a primary source of risk to children. This intense focus on the threat of maternal harm ignores significant environmental sources of injury, including fathers and other men, as well as exposure to toxic …

2013 Survey Of Juvenile Law, Michael J. Dale

Nova Law Review

No abstract provided.

No Entry To The Public Lands: Towards A Theory Of A Public Trust Servitude For A Way Over Abutting Private Land, Shelby D. Green

Elisabeth Haub School of Law Faculty Publications

This article explores the problem of inadequate access and why owners of private property abutting public lands cannot fence out the public if their sole or primary purpose is to deny access to public land. The reasons why such landowners should not be allowed to put up fences, even on their own land, if the effect is to hinder the public's access to public land are several. First, it is opportunistic and unjustly interferes with citizens' ability to enjoy the interest they hold in public lands. Second, it denies citizens access rights rooted in the common law. Third, and perhaps …

A Critical Research Agenda For Wills, Trusts And Estates, Bridget J. Crawford

Elisabeth Haub School of Law Faculty Publications

The law of wills, trusts, and estates could benefit from consideration of its development and impact on people of color; women of all colors; lesbian, gay, bisexual, and transgender individuals; low-income and poor individuals; the disabled; and nontraditional families. One can measure the law's commitment to justice and equality by understanding the impact on these historically disempowered groups of the laws of intestacy, spousal rights, child protection, will formalities, will contests, and will construction; the creation, operation and construction of trusts; fiduciary administration; creditors' rights; asset protection; nonprobate transfers; planning for incapacity and death; and wealth transfer taxation. This Article …

Intellectual Property And Public Health - A White Paper, Ryan Vacca

Faculty Publications

On October 26, 2012, The University of Akron School of Law's Center for Intellectual Property and Technology hosted its Sixth Annual IP Scholars Forum. In attendance were thirteen legal scholars with expertise and an interest in IP and public health who met to discuss problems and potential solutions at the intersection of these fields. This report summarizes this discussion by describing the problems raised, areas of agreement and disagreement between the participants, suggestions and solutions made by participants, and the subsequent evaluations of these suggestions and solutions.

Led by the moderator, participants at the Forum focused generally on three broad …

Cyclic Peptide-Capped Gold Nanoparticles For Enhanced Sirna Delivery, Amir Nasrolahi Shirazi, Karissa L. Paquin, Niall G. Howlett, Dindyal Mandal, Keykavous Parang

Pharmacy Faculty Articles and Research

Previously, we have reported the synthesis of a homochiral L-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]5 and [WR]5-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed …

Catalog (2014-2015), Philadelphia College Of Osteopathic Medicine

Catalogs

A snapshot of the PCOM curriculum through the decades.

South Dakota State University Undergraduate General Catalog 2014-2015, South Dakota State University

South Dakota State University Catalogs and Bulletins: 1935 to present

No abstract provided.