Digital Commons Network^™

Lack Of Negatively Charged Residues At The External Mouth Of Kir2.2 Channels Enable The Voltage-Dependent Block By External Mg2+, Junwei Li, Xiaoxiao Xie, Jun Liu, Hui Yu, Suhua Zhang, Yong Zhan, Hailin Zhang, Diomedes E. Logothetis, Hailong An

Physiology and Biophysics Publications and Presentations

Kir channels display voltage-dependent block by cytosolic cations such as Mg2+ and polyamines that causes inward rectification. In fact, cations can regulate K channel activity from both the extracellular and intracellular sides. Previous studies have provided insight into the up-regulation of Kir channel activity by extracellular K+ concentration. In contrast, extracellular Mg2+ has been found to reduce the amplitude of the single-channel current at milimolar concentrations. However, little is known about the molecular mechanism of Kir channel blockade by external Mg2+ and the relationship between the Mg2+ blockade and activity potentiation by permeant K+ ions. In this study, we applied …

Suppression Of The Pi3k Pathway In Vivo Reduces Cystitis-Induced Bladder Hypertrophy And Restores Bladder Capacity Examined By Magnetic Resonance Imaging, Zhongwei Qiao, Chunmei Xia, Shanwei Shen, Frank D. Corwin, Miao Liu, Ruijuan Guan, John R. Grider, Li-Ya Qiao

Physiology and Biophysics Publications and Presentations

This study utilized magnetic resonance imaging (MRI) to monitor the real-time status of the urinary bladder in normal and diseased states following cyclophosphamide (CYP)-induced cystitis, and also examined the role of the phosphoinositide 3-kinase (PI3K) pathway in the regulation of urinary bladder hypertrophy in vivo. Our results showed that under MRI visualization the urinary bladder wall was significantly thickened at 8 h and 48 h post CYP injection. The intravesical volume of the urinary bladder was also markedly reduced. Treatment of the cystitis animals with a specific PI3K inhibitor LY294002 reduced cystitis-induced bladder wall thickening and enlarged the intravesical volumes. …

In Pursuit Of Calmer Waters: Managing The Impact Of Trauma Exposure On Immigration Adjudicators, Kate Aschenbrenner

Faculty Scholarship

No abstract provided.

Fertility Preservation For Medical Reasons And Reproductive Justice, Seema Mohapatra

Faculty Scholarship

No abstract provided.

Reproduction And Population Of Porites Divaricata At Rodriguez Key: The Florida Keys, Usa, John Mcdermond

HCNSO Student Theses and Dissertations

Sexual reproduction in Porites divaricata (Le Sueur, 1820) was studied over a one year period (August, 2012 to August, 2013) in a shallow water (~2m depth) carbonate bank (also described as marginal) habitat at Rodriguez Key, a small island located 1.44 km off the east coast of Key Largo. Porites divaricata is a brooding, gonochoric species with peak reproductive output (planula release) occurring in March and at least a smaller event in May. This species showed an overall female to male sex ratio of 3.5:1. However, during peak reproduction, the sex ratio was 1.2:1. Spawning (sperm release) most likely occurred …

Florida-Friendly Landscaping(Tm) Right Plant, Right Place; Integrated Pest Management; And Attracting Wildlife, John J. Pipoly Iii

Marine & Environmental Sciences Faculty Reports

No abstract provided.

Age And Growth Of Three Coastal-Pelagic Tunas (Actinopterygii: Perciformes: Scombridae) In The Florida Straits, Usa: Blackfin Tuna, Thunnus Atlanticus, Little Tunny, Euthynnus Alletteratus, And Skipjack Tuna, Katsuwonus Pelamis, Jessica L. Adams, David W. Kerstetter

Marine & Environmental Sciences Faculty Articles

Background. Understanding the life history of a species is essential for fully understanding its role within an ecosystem. However, many of the fish species of high ecological value have not been studied due to their less prominent roles in local recreational and commercial fisheries in comparison to other targeted species. These valuable fishes are also important trophic linkages between small neritic fishes and large, economically valuable apex predators. This study describes for the first time the yearly age and growth patterns of three small tuna species inhabiting South Florida (USA) waters: blackfin tuna, Thunnus atlanticus (Lesson, 1831); little tunny, …

Beyond The Content And Pedagogy : A Step Forward Towards Value Based Teacher’S Professional Development, Sharifullah Baig

Professional Development Centre, Gilgit

This paper reviews the contemporary literature of human values and valuation processes, and proposes a re-conceptualized model of a teacher’s professional development. This paper suggests a thorough analysis of the teacher’s personality and devises alternative, well thought-out strategies and approaches to alter self-knowledge, values, valuation processes, and beliefs to imbed the desired impact on their teaching practices. This paper supports the use of deliberate attempts to alter the basic motivation level of teachers and to deliberately expose them to available knowledge to modify their values, attitudes, and actions in their respective professions, organizations, and formal associations. Hence, such professional development …

The History Manifesto, Jo Guldi

History Faculty Publications

How should historians speak truth to power -- and why does it matter? Why is five hundred years better than five months or five years as a planning horizon? And why is history -- especially long-term history -- so essential to understanding the multiple pasts which give rise to our conflicted present? The History Manifesto is a call to arms to historians and everyone interested in the role of history in contemporary society.

Co-Occurrence Of The Cyanotoxins Bmaa, Daba And Anatoxin-A In Nebraska Reservoirs, Fish, And Aquatic Plants, Maitham Ahmed Al-Sammak, Kyle D. Hoagland, David A. Cassada, Daniel D. Snow

Nebraska Water Center: Faculty Publications

Several groups of microorganisms are capable of producing toxins in aquatic environments. Cyanobacteria are prevalent blue green algae in freshwater systems, and many species produce cyanotoxins which include a variety of chemical irritants, hepatotoxins and neurotoxins. Production and occurrence of potent neurotoxic cyanotoxins β-N-methylamino-L-alanine (BMAA), 2,4-diaminobutyric acid dihydrochloride (DABA), and anatoxin-a are especially critical with environmental implications to public and animal health. Biomagnification, though not well understood in aquatic systems, is potentially relevant to both human and animal health effects. Because little is known regarding their presence in fresh water, we investigated the occurrence and potential for bioaccumulation of cyanotoxins …

Balancing Self-Defense And Mission Accomplishment In International Intervention: Challenges In Drafting And Implementing Rules Of Engagement, Todd C. Huntley

Maryland Journal of International Law

No abstract provided.

The Politics Of Religious Freedom In Malaysia, Tamir Moustafa

Maryland Journal of International Law

No abstract provided.

Unlawful Religion? Modern Secular Power And The Legal Reasoning In The Jfs Case, Peter Danchin, Louis Blond

Maryland Journal of International Law

No abstract provided.

Synthesis And Characterization Of Creka-Conjugated Iron Oxide Nanoparticles For Hyperthermia Applications, Anastasia M. Kruse, Samantha A. Meenach, Kimberly W. Anderson, J. Zach Hilt

Chemical, Biomolecular, and Materials Engineering Faculty Publications

One of the current challenges in the systemic delivery of nanoparticles in cancer therapy applications is the lack of effective tumor localization. Iron oxide nanoparticles coated with crosslinked dextran were functionalized with the tumor homing peptide CREKA, which binds to fibrinogen complexes in the extracellular matrix of tumors. This allows for the homing of these nanoparticles to tumor tissue. The iron oxide nanoparticle core allows for particle heating upon exposure to an alternating magnetic field (AMF) while the dextran coating stabilizes the particles in suspension and decreases the cytotoxicity of the system. Magnetically mediated hyperthermia (MMH) allows for the heating …

Progress Towards A Molecular Phylogeny Of Tachinidae, Year Two, Isaac S. Winkler, John O. Stireman Iii, John K. Moulton, Pierfilippo Cerretti, Jeremy D. Blaschke

Biological Sciences Faculty Publications

We reported last year (Stireman et al. 2013) in The Tachinid Times on our collaborative project, funded by the U.S. National Science Foundation, to study the phylogeny of Tachinidae on a worldwide scale. Since that report, we have made some significant progress towards our goal of assembling a robust phylogenetic tree for Tachinidae. Notably, Cerretti et al. (in press) completed a phylogenetic study using 135 morphological characters from 180 tachinid genera. Results from this study largely reflect, with some interesting exceptions, what we are finding from preliminary analyses of our molecular data. Although sampling of taxa and genes for the …

Big Data And Due Process: Toward A Framework To Redress Predictive Privacy Harms, Kate Crawford, Jason M. Schultz

Faculty Articles

The rise of “Big Data” analytics in the private sector poses new challenges for privacy advocates. Through its reliance on existing data and predictive analysis to create detailed individual profiles, Big Data has explod-ed the scope of personally identifiable information (“PII”). It has also effectively marginalized regulatory schema by evading current privacy protections with its novel methodology. Furthermore, poor execution of Big Data methodology may create additional harms by rendering inaccurate profiles that nonetheless impact an individual’s life and livelihood. To respond to Big Data’s evolving practices, this Article examines several existing privacy regimes and explains why these approaches inadequately …

University Of Dayton Magazine, Winter 2013-14, University Of Dayton Magazine

University of Dayton Alumni Publications

Up all night photo essay, law professor's research on social media, and history professor's research on KKK activities targeting Catholic schools.

Coca Leaves And Colorado: International Law And The Shifting Landscape Of Drug Reform, Cody T. Mason

Maryland Journal of International Law

No abstract provided.

Parenting: What's It Like For Black Fathers With Nonresident Children?, Katrina Ann Romaine Akande

Theses and Dissertations--Family Sciences

The demands on fathers to be more involved with their children have steadily been increasing since the 1970s. However, fathers have received less attention in the social science literature compared to mothers. This difference is evident regarding the parenting practices of ethnic minority fathers such as nonresident Black fathers. This dissertation investigates the effects of nonresident Black fathers’ perceptions of the coparenting relationship and coparenting support on their perceived father involvement and their perceived paternal stressors; while testing the mediating effects of maternal gatekeeping. The dissertation is presented in a three manuscript-style format with the intent to publish each manuscript …

Changes In Gene Expression And Viral Titer In Varroa Jacobsoni Mites After A Host Shift Asian To European Honey Bees, Gladys K. Andino Bautista

Open Access Dissertations

Honey bees (Apis mellifera L.) are the most important insects for the pollination of crops and wildflowers. However, they have experienced increasing colony die-offs during the past two decades. Multiple species of parasitic mites have been described that affect honey bees. The most important species in beekeeping belong to the genus Varroa ( Varroa jacobsoniand Varroa destructor). Varroa mite parasitism of honey bees is thought to be the most significant cause of colony mortality worldwide, and mite resistance to active ingredients of acaricides has become common. V. destructor causes direct impacts on bee production as well as …

The Voice, Winter/Spring 2014: Volume 59, Issue 2, Dordt College. Office Of College Advancement

The Voice Archive

No abstract provided.

Coastal Geology And Recent Origins For Sand Point, Lake Superior, Timothy G. Fisher, David E. Krantz, Mario R. Castaneda, Walter L. Loope, Harry M. Jol, Ronald J. Goble, Melinda C. Higley, Samantha Dewald, Paul R. Hanson

Department of Earth and Atmospheric Sciences: Faculty Publications

Sand Point is a small cuspate foreland located along the southeastern shore of Lake Superior within Pictured Rocks National Lakeshore near Munising, Michigan. Park managers’ concerns for the integrity of historic buildings at the northern periphery of the point during the rising lake levels in the mid-1980s greatly elevated the priority of research into the geomorphic history and age of Sand Point. To pursue this priority, we recovered sediment cores from four ponds on Sand Point, assessed subsurface stratigraphy onshore and offshore using geophysical techniques, and interpreted the chronology of events using radiocarbon and luminescence dating. Sand Point formed at …

Toward Enhanced Understanding And Projections Of Climate Extremes Using Physics-Guided Data Mining Techniques, A. R. Ganguly, E. A. Kodra, A. Agrawal, A. Banerjee, S. Boriah, Sn. Chatterjee, So. Chatterjee, A. Choudhary, D. Das, J. Faghmous, P. Ganguli, S. Ghosh, K. Hayhoe, C. J. Hays, W. Hendrix, Q. Fu, J. Kawale, D. Kumar, V. Kumar, W. Liao, S. Liess, R. Mawalagedara, V. Mithal, R. Oglesby, K. Salvi, P. K. Snyder, K. Steinhaeuser, D. Wang, D. Wuebbles

Department of Earth and Atmospheric Sciences: Faculty Publications

Extreme events such as heat waves, cold spells, floods, droughts, tropical cyclones, and tornadoes have potentially devastating impacts on natural and engineered systems and human communities worldwide. Stakeholder decisions about critical infrastructures, natural resources, emergency preparedness and humanitarian aid typically need to be made at local to regional scales over seasonal to decadal planning horizons. However, credible climate change attribution and reliable projections at more localized and shorter time scales remain grand challenges. Long-standing gaps include inadequate understanding of processes such as cloud physics and ocean–land–atmosphere interactions, limitations of physics-based computer models, and the importance of intrinsic climate system variability …

Strongly Bound Noncovalent (So3)N:H2co Complexes (N = 1, 2), L. M. Azofra, I. Alkorta, Steve Scheiner

Chemistry and Biochemistry Faculty Publications

The potential energy surfaces (PES) for the SO3:H2CO and (SO3)2:H2CO complexes were thoroughly examined at the MP2/aug-cc-pVDZ computational level. Heterodimers and trimers are held together primarily by SO chalcogen bonds, supplemented by weaker CHO and/or OC bonds. The nature of the interactions is probed by a variety of means, including electrostatic potentials, AIM, NBO, energy decomposition, and electron density redistribution maps. The most stable dimer is strongly bound, with an interaction energy exceeding 10 kcal mol(-1). Trimers adopt the geometry of the most stable dimer, with an added SO3 molecule situated so as to interact with both of the original …

Pharmacological Targeting Of The Mammalian Clock Regulates Sleep Architecture And Emotional Behaviour, Subhashis Banerjee, Yongjun Wang, Laura A. Solt, Kristine Griffett, Melissa Kazantzis, Ariadna Amador, Bahaa M. El-Gendy, Salvador Huitron-Resendiz, Amanda J. Roberts, Youseung Shin, Theodore M. Kamenecka, Thomas P. Burris

Department of Nutrition and Health Sciences: Faculty Publications

Synthetic drug-like molecules that directly modulate the activity of key clock proteins offer the potential to directly modulate the endogenous circadian rhythm and treat diseases associated with clock dysfunction. Here we demonstrate that synthetic ligands targeting a key component of the mammalian clock, the nuclear receptors REV-ERBα and β, regulate sleep architecture and emotional behaviour in mice. REV-ERB agonists induce wakefulness and reduce REM and slow-wave sleep. Interestingly, REV-ERB agonists also reduce anxiety-like behaviour. These data are consistent with increased anxiety-like behaviour of REV-ERBβ-null mice, in which REV-ERB agonists have no effect. These results indicate that pharmacological targeting of REV-ERB …

Simultaneous Phase And Scatter Correction For Nmr Datasets, Bradley Worley, Robert Powers

Robert Powers Publications

Nuclear magnetic resonance (NMR) spectroscopy has proven invaluable in the diverse field of chemometrics due to its ability to deliver information-rich spectral datasets of complex mixtures for analysis by techniques such as principal component analysis (PCA). However, NMR datasets present a unique challenge during preprocessing due to differences in phase offsets between individual spectra, thus complicating the correction of random dilution factors that may also occur. We show that simultaneously correcting phase and dilution errors in NMR datasets representative of metabolomics data yields improved cluster quality in PCA scores space, even with significant initial phase errors in the data.

The Current State Of Drug Discovery And A Potential Role For Nmr Metabolomics, Robert Powers

Robert Powers Publications

The pharmaceutical industry has significantly contributed to improving human health. Drugs have been attributed to both increasing life expectancy and decreasing health care costs. Unfortunately, there has been a recent decline in the creativity and productivity of the pharmaceutical industry. This is a complex issue with many contributing factors resulting from the numerous mergers, increase in out-sourcing, and the heavy dependency on highthroughput screening (HTS). While a simple solution to such a complex problem is unrealistic and highly unlikely, the inclusion of metabolomics as a routine component of the drug discovery process may provide some solutions to these problems. Specifically, …

Metabolomics Analysis Identifies D-Alanine-D-Alanine Ligase As The Primary Lethal Target Of D-Cycloserine In Mycobacteria, Steven M. Halouska, Robert J. Fenton, Denise K. Zinniel, Darrell D. Marshall, Raul G. Barletta, Robert Powers

Robert Powers Publications

D-cycloserine is an effective second line antibiotic used as a last resort to treat multi (MDR)- and extensively (XDR)- drug resistant strains of Mycobacterium tuberculosis. D-cycloserine interferes with the formation of peptidoglycan biosynthesis by competitive inhibition of Alanine racemase (Alr) and D-Alanine-D-alanine ligase (Ddl). Although, the two enzymes are known to be inhibited, the in vivo lethal target is still unknown. Our NMR metabolomics work has revealed that Ddl is the primary target of DCS, as cell growth is inhibited when the production of D-alanyl-Dalanine is halted. It is shown that inhibition of Alr may contribute indirectly by lowering …

Water Quantity And Quality In The Columbia Basin Trust Region, Janice Brahney

Watershed Sciences Faculty Publications

This report collects the documents generated as part of a two year effort to catalogue the variety of water quality and quantity data that has been collected in the Columbia Basin Trust region of British Columbia. Through the cataloguing effort, a number of important data and knowledge gaps were identified and specific recommendations developed. The available water quantity and climate data was then used in a number of analyses focused on understanding the relationship between changing climate conditions and stream discharges.

One overall finding is that there is a large variety of data collected and held by various different entities. …

Classical Flt3l-Dependent Dendritic Cells Control Immunity To Protein Vaccine, Niroshana Anandasabapathy, Rachel E. Feder, Shamim A. Mollah

Publications

DCs are critical for initiating immunity. The current paradigm in vaccine biology is that DCs migrating from peripheral tissue and classical lymphoid-resident DCs (cDCs) cooperate in the draining LNs to initiate priming and proliferation of T cells. Here, we observe subcutaneous immunity is Fms-like tyrosine kinase 3 ligand (Flt3L) dependent. Flt3L is rapidly secreted after immunization; Flt3 deletion reduces T cell responses by 50%. Flt3L enhances global T cell and humoral immunity as well as both the numbers and antigen capture capacity of migratory DCs (migDCs) and LN-resident cDCs. Surprisingly, however, we find immunity is controlled by cDCs and actively …