Digital Commons Network^™

Producing An Ovine Model Of Cystic Fibrosis, Kira Perry Morgado

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cystic Fibrosis (CF) is an autosomal recessive disease that significantly affects quality of life and lifespan. There are currently no effective animal models of CF that mimic the human disease state. This prevents the development of pharmaceutical treatments for patients. Sheep have been considered for a useful animal model because of their size, life expectancy, and similarities in their anatomy and physiology. In order to generate a sheep transgenic model to study CF we have produced two Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene targeting DNA vectors containing large regions of homology to the CFTR gene in sheep. One of …

Ghrelin Concentrations In Milk And Plasma Of Dairy Cows During Early Lactation, Sameer M. Alhojaily

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ghrelin is a hormone produced mainly by the cells lining the gastric mucosa. Ghrelin was first extracted from human and rat stomachs, and identified as an endogenous stimulator of growth hormone release. Ghrelin is synthesized and produced in several tissues, but the gastric mucosa remains the major source of circulating ghrelin. Besides growth hormone release, ghrelin stimulates appetite and plays some major roles in different organs. In several studies, ghrelin was described as a hormone with multiple functions and diverse biological actions. Ghrelin exists in two major forms, active ghrelin and inactive ghrelin, and only the active from binds to …

Effects Of Processing And Packaging Modifications On The Quality And Shelf Life In Meats, Abdulla Khan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Shelf life of foods, mainly meat and meat products, is affected by its physical and chemical properties like color, pH, water holding capacity etc. In developed countries
food losses occur at production, retail and consumer levels with meat commodities accounting for 41% of the total loss in US during 2008. These losses are because of
spoilage due to inappropriate packaging, improper storage conditions, food wastage, and lack of consumer awareness. By focusing on shelf life and quality issues at the production level, we can overcome some of the major problems faced by the food manufacturers.

Active packaging is an innovative …

Self-Reported Psychopathology Correlates Of Homeless Youth In Utah, Kimberlee Taylor

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Homelessness among unaccompanied youth is a unique, yet pervasive, social problem. Youth often become homeless through three central pathways: conflict with family, involvement in the foster system, and involvement in juvenile justice systems. As youth experience homelessness during important developmental period(s), vulnerability to mental illness may occur if not already present. The present study examined the type and prevalence of mental illness. Characteristics of homelessness, health and mental health service utilization, and pathways to homelessness were examined in relation to the occurrence of mental illness. Findings indicated that a variety of characteristics are associated with mental illness. Mental health service …

Specific Learning Disabilities: Beliefs About The Construct, Identification Methods, And Job Satisfaction Among Practicing School Psychologists, Joseph M. Cottrell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Students with specific learning disabilities (SLDs) account for approximately 40% of all students receiving special education services. Debate among professionals regarding the causes of SLDs and the most appropriate methods used to identify SLDs persists. This debate may be related to the increase in prevalence of SLDs since the implementation of special education law in 1975. There are three prominent theories regarding the cause of SLDs: (a) environmental theory, (b) biological theory, and (c) interactional theory. The Individuals with Disabilities Education Act (IDEA) allows school districts to implement the following SLD identification procedures: (a) the IQ-Achievement discrepancy method, (b) response-to-intervention …

A Survey Of Utah's Public Secondary Education Science Teachers To Determine Their Feelings Of Preparedness To Teach Engineering Design, Roland Tyler Ames

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Education is always changing and science education is no exception, with many influential publications passing through science education over the years. The latest wave in science standards is called the Next Generation Science Standards. The Next Generation Science Standards are anticipated to have a significant effect on state science standards around the entire country. One thing about these new standards is very different from all previous science standards—they include the principle of engineering design in them.

Asking science teachers to teach engineering design is asking them to teach a principle for which their teaching licensure would not have formally prepared …

A Preliminary Study Of The Role Of Nickel In Enhancing Flowering Of The Nickel Hyperaccumulating Plant Alyssum Inflatum Nyár. (Brassicaceae), R. Ghasemi, Z. Z. Chavoshi, R. S. Boyd, Nishanta Rajakaruna

Faculty Publications, Biological Sciences

Alyssum inflatum is a native of serpentine soils and is able to hyperaccumulate nickel (Ni), but the importance of Ni to reproduction in the species is unknown. We investigated if reproductive fitness is enhanced by Ni in the growth medium, and included a treatment involving a relatively high level of Mg to provide a comparison with elevated levels of another metal. Seedlings were grown in a modified Hoagland solution culture in an inertmediumof Perlite andwere treatedwith Ni (100 μM), a high concentration ofMg (5mM), or under control conditions (solution culture without Ni or the addition of high Mg) for 14 …

The Dimerization Domain In Dape Enzymes Is Required For Catalysis, Boguslaw Nocek, Anna Starus, Magdalena Makowska-Grzyska, Blanca Gutierrez, Stephen Sanchez, Robert Jedrzejczak, Jamey C. Mack, Kenneth W. Olsen, Andrzej Joachimiak, Richard C. Holz

Chemistry: Faculty Publications and Other Works

The emergence of antibiotic-resistant bacterial strains underscores the importance of identifying new drug targets and developing new antimicrobial compounds. Lysine and meso-diaminopimelic acid are essential for protein production and bacterial peptidoglycan cell wall remodeling and are synthesized in bacteria by enzymes encoded within dap operon. Thereforedap enzymes may serve as excellent targets for developing a new class of antimicrobial agents. The dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) convertsN-succinyl-L,L-diaminopimelic acid to L,L-diaminopimelic acid and succinate. The enzyme is composed of catalytic and dimerization domains, and belongs to the M20 peptidase family. To understand the specific role …

Acoustic Estimates Of Methane Gas Flux From The Seabed In A 6000 Km2 Region In The Northern Gulf Of Mexico, Thomas C. Weber, Larry A. Mayer, Kevin W. Jerram, Jonathan Beaudoin, Yuri Rzhanov

Center for Coastal and Ocean Mapping

Seeps of free methane gas escaping the seabed can be found throughout the ocean basins. To understand the role of methane gas seeps in the global carbon cycle—including both gas added to the atmosphere and that which is dissolved and potentially oxidized in the ocean volume—it is important to quantify the amount of methane escaping the seabed. Few large-scale mapping projects of natural methane seeps have been undertaken, however, and even among these, quantitative estimates of flux are rare. Here we use acoustic mapping techniques to survey 357 natural methane seeps in a large region (6000 km2) of the northern …

Clpxp Modulates Cell Growth And Morphology In Cell Shape Mutants Of E.Coli, Ryann Murphy

Senior Honors Projects

ClpXP modulates cell growth and morphology in cell shape mutants of E. coli

Ryann Murphy¹ and Jodi L. Camberg¹

¹University of Rhode Island, Department of Cell and Molecular Biology, Kingston, RI, 02881

Penicillin Binding Proteins (PBPs) are a family of prokaryotic membrane proteins named for their propensity to bind the antibiotic penicillin and are involved in remodeling and deposition of peptidoglycan. In wild type Escherichia coli cells, the uniform rod shape is conserved across generations. E.coli cells containing multiple deletions of Low Molecular Weight (LMW) PBPs exhibit irregular shapes. LMW PBP5 (dacA) is a potential …

The Grizzly, May 1, 2014, Dave Muoio, Deana Harley, Jonathan Vander Lugt, Larissa Coyne, Olivia Z. Schultz, Evan Hill, Sophie Snapp, Shawn Hartigan, John Carty, Dana Feigenbaum, Giovanni Waters, Karch Connors

Ursinus College Grizzly Newspaper, 1978 to Present

Faculty Start First Creative Art Exchange at Myrin Library • UC Sustainability and Graduation Speaker Important to Upcoming Commencement • Safety and Care Remain Important RA Qualities • Bearitones and B'Naturals Will Take the Stage This Weekend for Semesterly Concerts • Ursinus Dance Company to Take the Stage This Weekend • Soccer Tournament Supports Ragball • Fulbright Winner Will Travel to Spain • Opinion: Four Loko-Type Drinks Shouldn't be Banned; Temple Cuts Cast Shadow Over Men's Gymnastics • Opinion: NFL Players Earn Every Cent • NBA Owner Accused of Making Racist Comments • Men's Lacrosse, Both Tennis Teams Finish Strong

Serum 25-Hydroxyvitamin D, Calcium, And Calcium-Regulating Hormones In Preeclamptics And Controls During First Day Postpartum, Ahmed Dalmar, Hershel Raff, Suneet P. Chauhan, Maharaj Singh, Danish S. Siddiqui

College of Nursing Faculty Research and Publications

The evidence for a link between vitamin D and preeclampsia is conflicting. There is a paucity of studies reporting simultaneous 25-hydroxyvitamin D (inactive form) and 1,25-dihydroxyvitamin D (biologically active form). We investigated if levels of serum 25-hydroxyvitamin D, calcium-regulating hormones (1,25-dihydroxyvitamin D, parathyroid hormone), and calcium differ significantly between preeclamptics and controls. On postpartum day one, 98 subjects (44 with preeclampsia, 54 controls) were recruited among women admitted to the postdelivery unit, and their serum 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, parathyroid hormone, serum calcium, and serum albumin levels were prospectively measured. The majority of participants (70 %) had serum 25-hydroxyvitamin D …

Cohort Efficacy Study Of Natural Family Planning Among Perimenopause Age Women, Richard J. Fehring, Qiyan Mu

College of Nursing Faculty Research and Publications

Objective: To determine the efficacy of using natural family planning (NFP) methods to avoid unintended pregnancy among women of perimenopause age (i.e., age 40-55 years).

Design: A secondary analysis of subset data from two prospective observational cohort studies.

Setting: A university based in-person and online NFP service program.

Participants: One hundred and sixty couples who used either a website or an in-person NFP service to learn how to avoid pregnancy from January 2001 to November 2012.

Methods: A prospective 12-month effectiveness study among 160 women (between ages 40-55) who used NFP to avoid pregnancy. The …

Feasibility Of Oral Prenatal Probiotics Against Maternal Group B Streptococcus Vaginal And Rectal Colonization, Lisa Hanson, Leona Vandevusse, Megan Duster, Simone Warrack, Nasia Safdar

College of Nursing Faculty Research and Publications

Objective

To examine the effect of an oral prenatal probiotic on group B Streptococcus (GBS) colonization and to demonstrate the feasibility of a larger randomized controlled trial.

Design

This pilot study was an open‐label, two‐group quasi‐experiment.

Setting

An urban central city nurse‐midwifery and wellness center serving a diverse population.

Participants

Ten pregnant participants received the oral probiotic (Florajen3) taken once daily, and 10 participants served as controls.

Methods

A questionnaire on dietary practices, vaginal cleansing, sexual history, and symptoms and GBS colony count samples were taken at 28‐, 32‐, and 36‐weeks gestation.

Results

Participants in the probiotic group reported no …

The Spatial Relationship Between Crime And Public Transportation: A Geospatial Analysis Of Salt Lake City's Trax System, Joel W. Warren

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

It is well-known that, when it comes to crime, some neighborhoods are safer than others. Researchers who make maps of crime have observed that some areas of cities have more crime than others. These areas of high crime are often called hot spots. Crime pattern theory explained why some neighborhoods have more crime than others by looking at criminal events as a meeting between a motivated criminal and a target. Social scientists, geographers, and city planners have shown that criminals generally choose targets from places they see every day, for example on their ride to work or the grocery store. …

Modeling To Improve Vegetation-Based Wetland Biological Assessment, Robin C. Jones

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To meet the requirements of the Clean Water Act (1972), natural resource managers need to be able to detect biological degradation in wetland ecosystems. Biological indices are commonly used by managers to assess wetland biological condition. The accuracy and precision of wetland condition assessments are directly related to the performance of these indices, and biological index performance is thought to be related to how well an index controls for the effects of environmental attributes on biological assemblages. Many plant-based biological indices control for environmental and biological variation through the use of classification schemes that are based on geographic location and …

An Evaluation Of The Relative Performance Of Diploid Versus Triploid Brook Trout With Consideration Of The Influence Of Lake Characteristics, Andy Dean

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Brook trout are native to the east and Midwestern United States. Brook trout have become a popular sport fish in the western United States and are currently widely stocked to provide sport fishing opportunities throughout the west. The Utah Division of Wildlife Resources (UDWR) regularly stocks brook trout into high mountain lakes in the Uinta Mountains to provide one of these popular fisheries. Stocking non-natives to high mountain lakes can cause competition with native species for food and habitat resources and provides source populations for dispersal of non-natives downstream. Triploid (sterile) fish may provide an opportunity to stock non-native fish …

Linking Temporal And Spatial Variability Of Millennial And Decadal-Scale Sediment Yield To Aquatic Habitat In The Columbia River Basin, Elijah Portugal

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Historically the Columbia River was amongst the most productive salmonidbearing watersheds in North America. Currently, salmonid populations have collapsed and are estimated at ten percent of historic levels. Because of this, there are thirteen, Endangered Species Act (ESA) listed distinct population segments within the Columbia River Basin (CRB). The Bonneville Power Administration (BPA) operates a substantial number of hydroelectric dams within the CRB. As the dams effectively block anadromy and are thought to be extremely detrimental to the long-term persistence of salmonid populations within the CRB, the BPA funds a series of programs to mitigate the impacts of dam operation. …

Mothers On The Market: Employer Hiring Practices And Motherhood Penalties, Elizabeth Kiester

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

While gender inequity in wages is often discussed in the media by politicians, the motherhood wage penalty receives substantially less attention. This distinct wage penalty suggests that women with children suffer a wage penalty separate from women without children. In addition, there is an employment gap between mothers and nonmothers indicating that mothers are less likely to be employed. While some argue that this is a matter of choice, I contend that mothers may face unique barriers that deny them access to the labor market. I tested this theory in two ways and in two states; Utah and California. First, …

Molecular Systematics, Historical Biogeography, And Evolution Of Spider Wasps (Hymenoptera: Pompilidae), Juanita Rodriguez

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The study of the diversity and classification of any group of organisms provides a foundation for further scientific studies in ecology, evolution, and conservation. Insects are among the most diverse organisms that inhabit the planet, but knowledge of their diversity and classification is still limited. One understudied group of insects is spider wasps. These are solitary parasitoids that use one spider to lay a single egg. There are approximately 5,000 described species, and many more to be described. Unfortunately, fewer than 10 scientists worldwide study these insects. One reason the group has not been very well studied is the difficulty …

Exploring The Lived Experiences Of Couples Making The Transition To Parenthood And The Meaning They Ascribe To Brief, Couple-Focused, Preventative Interventions, Dolores D. Michael

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The objective of this research study was to investigate how meaningful it would be to provide relationship therapy from a marriage and family therapist to couples going through the transition to parenthood. The participants in this study all received four therapy sessions before the baby was born and one therapy session after the birth. A total of five couples participated in this study. Data was gathered from the assessments they took before each therapy session and at the final interview.

As a result, the participants in this study all remained stable or increased in their marital satisfaction when measured four …

An Adolescent Journey: Expressive Letter Writing Through A Wilderness Adventure Therapy Program, Ava M. Crump

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This qualitative phenomenological study explored the key themes of an adolescent journey during a wilderness adventure therapy program through expressive letter writing to their parents. Ten complete sets of letters (five boys and five girls) totaling over 400 pages were analyzed by four independent coders until saturation of themes were reached. There were five overarching themes that emerged from the data: impact of wilderness experiences, desires for improved relationships, apology and accountability, negative emotions, and positive growth and coping. These themes were presented in the chronological pattern that they appeared in the letters. The findings represent the adolescents’ experiences written …

Pathway Pioneer: A Web-Based Graphical Tool For The Organization And Flux Analysis Of Metabolic Networks, Sumit Kumar Singh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Every so often the field of genome-scale metabolic networks undergoes radical changes, each bringing its own complexity and challenges. The existing visualization tools, owing to their inability to assist in generating layouts suitable for easy readability and conceptualization, hinder further advancement. The lack of in-depth comprehensible representation of the intricacies of interaction and arrangements within the network topology prevents researchers from being able to advance at a faster speed despite the growing number of networks and systems. The far and wide diverse standards, layout schemas and network formats of the biological realm contribute to the existing challenges of developing visualization …

Pathway Pioneer: A Web-Based Metabolic Network Layout Extension, Harsh Dosi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The number and complexity of genome-scale metabolic networks have seen a rapid growth in the past 10 years. Today, more than 450 genome-scale metabolic reconstructions of biological systems are characterized and existing models are extended. With the growing influence of these reconstructions on biomedical and biological research, the field has observed a shift from an inward focus on method development to an outward focus on application development.

Static state analysis of metabolic networks in textual format is too cumbersome and time consuming. In addition, visualization and arrangement of these networks to facilitate their analysis is an open research problem. A …

Father-Child Play Behaviors And Child Emotion Regulation, Amanda Hagman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Most of us have learned ways to manage how, when, and to what extent we experience emotions. These emotion regulation skills are habitual and may seem innate, but for infants and young children these abilities are basic, limited, and unrefined. Developing emotion regulation skills is an important learning task for infants and toddlers; the role of fathers in emotion regulation learning is a growing area of research. This research takes a closer look at early childhood and how father-child interactions may contribute to child emotion regulation.

Theory suggests that during interactions with their parents, children learn emotion regulation skills, but …

A Quantitative Approach To The Development Of Ecological Sites And State-And-Transition Models, Matthew W. Van Scoyoc

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Changes in land-use and climate can trigger changes in ecosystem conditions and may be driving ecosystems toward undesired “states” that provide inadequate ecosystem services. If these changes are drastic enough, energy intensive restoration programs are necessary to restore ecosystems to previous states. Landscape classification systems based on “ecological potential” offer a robust framework to evaluate and manage ecosystems. The ecological site concept is one such landscape classification system that has been developed by the USDA Natural Resources Conservation Service (NRCS) and describes ecosystems and ecosystem dynamics relative to “reference conditions” and response to disturbance. Ecological sites have gained popularity with …

Prediction Of Climate Change Effects On Streamflow Regime Important To Stream Ecology, Sulochan Dhungel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Streamflow is expected to change with climate change, but a general understanding of how climate change will affect the characteristics of streamflow important to stream ecosystems is lacking. This thesis focused on specific characteristics of streamflow, or the streamflow regime, that are important to stream ecosystems. The need to evaluate the potential impacts of climate change on stream ecosystems makes it important to study how streamflow regime may change. This work has identified 16 characteristics of daily streamflow time series that quantify the patterns and span the aspects of streamflow regimes important for stream ecosystems. Principal component analysis objectively identified …

Structure And Multi-Center Bonding: From Atomic Clusters To Solid Phase Materials, Timur R. Galeev

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The work presented in this dissertation has been focused on structure, stability, and chemical bonding of atomic clusters and solid-state compounds. The common thread was development of theoretical models and methods capable of interpreting bonding and hence, structures of these species. A major part of this work presents joint experimental and theoretical studies of a large series of doped boron clusters. The dissertation introduces a new theoretical tool for interpretation of bonding of solid phase materials in chemically intuitive terms of localized and multi-center bonds. Understanding how interactions between atoms in sub-nano clusters and solid-state compounds determine their structures, physical …

Computational Models Of Intracellular And Intercellular Processes In Developmental Biology, Ahmadreza Ghaffarizadeh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Systems biology takes a holistic approach to biological questions as it applies mathematical modeling to link and understand the interaction of components in complex biological systems. Multiscale modeling is the only method that can fully accomplish this aim. Mutliscale models consider processes at different levels that are coupled within the modeling framework. A first requirement in creating such models is a clear understanding of processes that operate at each level. This research focuses on modeling aspects of biological development as a complex process that occurs at many scales. Two of these scales were considered in this work: cellular differentiation, the …

Expert Vs. Novice: Problem Decomposition/Recomposition In Engineering Design, Ting Song

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this research was to investigate the design behavior. A commonly used design strategy called “problem decomposition and recomposition” was the focus of the study. Dyads of engineering experts, dyads of engineering seniors, and dyads of engineering freshmen completed an engineering design challenge within an hour. The entire design process was video and audio recorded. After the design session, participants participated in a group interview.

The conversation and behavior from the design were transcribed, segmented, and coded. Two coding systems including the FBS ontology and “levels of the problem” were used in this study. A series of statistical …