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Augmentation Of Ras-Induced Cell Transformation : A New Role For Mir-200a In Malignancy., Lindsey Erin Becker

Electronic Theses and Dissertations

Cancer is a multistep disease that begins with malignant cell transformation and frequently culminates in metastasis and death. MicroRNAs (miRNAs) are small regulatory 21-25-nt RNA molecules and are frequently deregulated in cancer. The majority of miRNAs are estimated to be co-expressed with neighboring miRNAs as clusters. Many miRNA clusters coordinately regulate multiple members of cellular signaling pathways or protein interaction networks. miR-200a is a member of the miR-200 family, which are known to be strong inhibitors of the epithelial to mesenchymal transition. As such, the tumor suppressive role of miR-200a in oncogenesis has been well studied; however, recent studies have …

Forecasting Seasonal Hydrologic Response In Major River Basins., A. M. Tanvir Hossain Bhuiyan

Electronic Theses and Dissertations

Seasonal precipitation variation due to natural climate variation influences stream flow and the apparent frequency and severity of extreme hydrological conditions such as flood and drought. To study hydrologic response and understand the occurrence of extreme hydrological events, the relevant forcing variables must be identified. This study attempts to assess and quantify the historical occurrence and context of extreme hydrologic flow events and quantify the relation between relevant climate variables. Once identified, the flow data and climate variables are evaluated to identify the primary relationship indicators of hydrologic extreme event occurrence. Existing studies focus on developing basin-scale forecasting techniques based …

Neutrophils Alter Epithelial Response To Porphyromonas Gingivalis In A Gingival Crevice Model., Jessica Lynn Bondy-Carey 1987-

Electronic Theses and Dissertations

A gingival crevice model (epithelial cell-Porphyromonas gingivalis-neutrophil) was established and used to profile gingipain, matrix metalloproteinase (MMP), MMP mediators [neutrophil gelatinase-associated lipocalin (NGAL) and tissue inhibitor of metalloproteinases 1 (TIMP-1)] and cytokine networks. Smoking is the primary environmental risk factor for periodontitis. Therefore, the influence of cigarette smoke extract (CSE) was also monitored in the same model. Porphyromonas gingivalis alone induced low levels of interleukin-1b and interleukin-8 from epithelial cells, but high levels of both cytokines were produced on the addition of neutrophils. Exposure to CSE (100 and 1000 ng mL1 nicotine equivalency) significantly compromised P. gingivalis-induced cytokine secretion (both …

Neurodegeneration-Associated Instability Of Ribosomal Dna., Justin Lauren Hallgren 1981-

Electronic Theses and Dissertations

Homologous recombination-mediated instability of the repetitively organized ribosomal DNA has been proposed as a mediator of cell senescence in yeast triggering the DNA damage response. High individual variability in the content of human ribosomal DNA suggests that this genomic region remained relatively unstable throughout evolution. Therefore, quantitative real time PCR was used to determine the genomic content of ribosomal DNA in post mortem samples of parietal cortex from 14 young and 9 elderly individuals with no diagnosis of a chronic neurodegenerative/neurological disease. In addition, ribosomal DNA content in that brain region was compared between 10 age-matched control individuals and 10 …

Soil Moisture And Temperature Conditions Related To Cheatgrass Establishment Failure, Lauren Ducas

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Cheatgrass, a widespread invasive plant, has taken over many rangelands in the Intermountain West. Restoration efforts have not met with much success. Early in cheatgrass’ growth period, if air temperatures fall too low and soils too dry, growth may be disrupted to the point of death of the cheatgrass plants. In this case, cheatgrass stands may revert to bare ground, providing opportunities for other invasive plants to establish, or possible restoration of native perennials. We conducted a computer modeling and observational field study to determine if air temperature and soil moisture conditions below its tolerance are associated with failure of …

Advancing Digital Soil Mapping And Assessment In Arid Landscapes, Colby W. Brungard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Soil information is required for arid and semi-arid land management decisions such as permitting livestock grazing or planning vegetation restoration projects. However, traditional soil mapping methods may not provide adequate soil information, because the scale of mapping often requires dissimilar soils to be grouped together and there are no estimates of map uncertainty. Traditional methods are also often too costly or impractical to implement in large, remote, public arid and semi-arid rangelands. Digital soil mapping (DSM) may be able to overcome these limitations. Digital soil mapping is the creation of pixel-based soil maps using quantitative statistical models that relate easily …

Restoring The Lost Fishery: An Environmental History Of Northern Nevada's Pyramid Lake And Lower Truckee River Fishery, David Bolingbroke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This thesis focuses on fisheries managers’ efforts to restore native cutthroats to northern Nevada’s Pyramid Lake for recreation, and the Paiutes’ battle to preserve them as a means of livelihood. Their efforts to reconstruct the fishery revealed the implausibility of environmental restoration, but more importantly underlined the motivations necessary to attempt it.

Chapter 2 describes how the Pyramid Lake Lahontan cutthroat— historically an important subsistence resource for Northern Paiutes— were initially exploited for profit in the late 1800s and early 1900s, and gradually destroyed as agricultural interests diverted the Truckee River’s water and industrial pollution contaminated the trout’s aquatic habitat. …

Foraging Ecology Of Cougars In The Pryor Mountains Of Wyoming And Montana, Linsey Blake

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

We conducted this study to better understand the impact of cougar (Puma concolor) predation in the Pryor Mountains of Wyoming and Montana. Managers of the Bighorn Canyon National Recreation Area and the Pryor Mountain Wild Horse Range were concerned that cougars were having a negative impact upon a small, isolated Rocky Mountain bighorn sheep (Ovis canadensis canadensis) population and were hoping predation might be limiting a feral horse population (Equus caballus) that was in excess of the Appropriate Management Level set by the Bureau of Land Management. Wildlife tourism brings revenue to the park …

Nutrition And Genes Associated With Orofacial Cleft Birth Defects In Utah, Huong Dieu Meeks

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Orofacial clefts (OFCs) are facial malformations that happen during early pregnancy and have a complex and heterogeneous etiology, involving both genetic and environmental risk factors. This project examined the association between maternal nutrition, folate-related biomarkers, candidate genes involved in one-carbon metabolism (OCM), and OFCs in order to achieve more comprehensive knowledge of how nutrition and genetics influence OFC risk. All studies performed in this project used the data collected in the Utah Child and Family Health Study (UCFHS) and the Johns Hopkins International Cleft Consortium.

Neither maternal periconceptional multivitamin use nor healthy dietary pattern score alone was individually associated with …

Habitat Characteristics Of Mexican Spotted Owls (Strix Occidentalis Lucida) In The Canyonlands Of Southern Utah, Leah R. Lewis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mexican Spotted Owls are considered a threatened species by the US Fish and Wildlife Service. Therefore, they must be properly managed and protected by federal and state agencies. In this study I explored the habitat characteristics used by Mexican Spotted Owls in the canyonlands region of southern Utah. I spent three summer field seasons (May-September) from 2010 – 2013 locating Mexican Spotted Owl sites and measuring vegetative and geologic features at these sites. I found that canyon width and very tall vegetation influenced Mexican Spotted Owl presence, suggesting that these owls prefer narrow canyons that are vegetated with trees.

Based …

The Ecosystem Role Of Fishes In Lotic Environments, Christopher C. Wheeler

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

It is important for humans to understand how ecosystems work because we depend on them for a variety of products and services. For example, rivers and streams provide fisheries, improved water quality, and recreational opportunities to many individuals. In rivers, interactions among fishes, other stream plants and animals, and the physical river environment can influence continued provision of these valuable services. However, the role played by many freshwater fishes in the provision of these services remains unknown. Additionally, it is important to identify different factors that affect the outcome of interactions involving riverine fishes. To address these issues, I evaluated …

Investigations Of Cho 1-15 And Silk Gland Cell Line Development, Susan Kathleen Robinson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The BioProcessing Demonstration and Training Laboratory was established with a collaboration between Utah State University and Thermo Fisher-Scientific, Inc. This lab was developed into a fully functioning tissue culture facility. Demonstration of tissue culture procedures were necessary for the lab to be industry-fully qualified. The CHO 1-15 cell line protocols were optimized by establishing conditions for reproducibility in shaker flasks and bioreactors (2 to 250 L capacity). CHO 1-15 is the cell line of choice for protein production in the bio-manufacturing industry. Oxidative stress is a problem in the industry because it can cause a decrease in protein production. Mesobiliverdin …

Barriers To Curricular Change In General Education Mathematics At A Large Public Institution, Marcus Jorgensen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This qualitative study looked for barriers to curricular change in general education mathematics at a large public university (LPU) in the Western United States. The literature showed that a number of national organizations dealing in math education have recommended alternatives to the traditional algebra-intensive curriculum. LPU still relies predominantly on traditional College Algebra as its quantitative literacy (QL) course. With such an algebra-heavy emphasis, students may be missing more relevant and useful mathematics. In addition, the College Algebra course has a low success rate, which becomes a barrier to student retention and graduation. Interviews were conducted with 14 participants who …

System Identification Of A Small Low-Cost Unmanned Aerial Vehicle Using Flight Data From Low-Cost Sensors, Nathan V. Hoffer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Remote sensing has usually been done with satellites and manned aircraft. While they can be useful, satellites and manned aircraft have issues in how quickly you can get data, process it, and go back to get more data. Small low-cost unmanned aerial vehicles (UAVs) overcome these issues. To collect precise data a precise model of the UAV is needed to develop the autopilot, make sure the aircraft flies well, and does not break. One method of making a model is system identification (system ID). In this thesis system ID of a small low-cost fixed-wing T-tail UAV is done. The equations …

Activated Carbon Decreases Invasive Plant Growth By Mediating Plant-Microbe Interactions, Nicole E. Nolan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To restore lands invaded by dense, non-native vegetation, it may be necessary to develop targeted restoration tools than can remove the mechanism used by these non-native species as a competitive advantage. Activated carbon (AC) is one such tool, with the ability to disrupt the mechanisms of plant to plant communication (allelopathy) and positive plant to microbe communication commonly used by non-native species. Previous studies have shown the success of high concentrations, 1000g/m², of AC in native plant community restoration on a small scale. Here, our goals are twofold: first, to test AC effectiveness in restoring desirable plant communities …

Implementation Of An Oral Health Intervention For Adults With Intellectual And Developmental Disabilities., Brigit Rene Zaksek

Electronic Theses and Dissertations

Often oral care is overlooked during the daily care of adults with intellectual and developmental disabilities (IDD). Due to the integration of adults with IDD from institutions into group homes and private homes, caregivers need to be taught to implement daily oral hygiene protocols. The purpose of this pilot study, conducted by the University of Louisville and the Pacific Institute for Research and Evaluation, was to develop an oral hygiene plan for adults with IDD residing in group homes in the Louisville, Kentucky area that would ultimately improve their oral health and quality of life. This thesis focuses on the …

Gender Stereotyping Within Career And Technical Education : Exploring Relationships Among Gender, Coursetaking, And Outcomes Of High School Cte Students., Stephanie A. Fluhr

Electronic Theses and Dissertations

This dissertation explored he relationships among gender, coursetaking, and student outcomes of high school CTE students. The variables analyzed within this study were selected based upon an extensive review of existing literature on gender, stereotypes, occupation, nontraditional careers, and CTE coursetaking and policy. The sample examined in this study came from a Midwestern state’s database of CTE students that were enrolled in high school CTE courses from 2010-2012. Operating under a theory that gender segregation with CTE leads to a continuance of gender segregation in the workforce at large, the purpose of this study was to add to the existing …

Enhancement Of Anaerobic Digestion Of Actual Industrial Wastewaters : Reactor Stability And Kinetic Modeling., Mahyar Ghorbanian

Electronic Theses and Dissertations

Industrial plants pay disposal costs for discharging their wastewater that can contain pollutants, toxic organics and inorganics, to the sewer based on the Biological Oxygen Demand (BOD) or Chemical Oxygen Demand (COD) of the streams. It has become increasingly expensive for industry to meet stringent regulatory standards. One solution to reduce this cost is to anaerobically degrade the COD content, which in turn generates useful methane gas that can be used to generate useful energy or heat. Anaerobic Digestion (AD) is one of the most suitable renewable resources of conversion of industrial wastewaters to bioenergy, but it is not widely …

A Male Spider’S Ornamentation Polymorphism Maintained By Opposing Selection With Two Niches, Bo Deng, Alex Estes, Brett Grieb, Douglas Richard, Brittney Hinds, Eileen Hebets

Eileen Hebets Publications

The Levene mechanism to maintain genotypic polymorphism by opposing selection on genotypes in multiple niches was proposed 60 years ago, and yet no systems were found to satisfy the mechanisms rather restrictive conditions. Reported here is such an example that a wolf spider population lives in a habitat of mixed rocks and leafy litter for which the females are phenotypically indistinguishable and the males have two distinct phenotypes subject to opposing selection with respect to the substrates. Census data is best-fitted to a population genetics model of the Levene type. A majority of the best fit support polymorphism, with many …

Tactical Adjustment Of Signaling Leads To Increased Mating Success And Survival, Laura Sullivan-Beckers, Eileen A. Hebets

Eileen Hebets Publications

Most sexually reproducing animals overcome the challenge of searching for and attracting mates by utilizing signals that are broadcast through a spatially and temporally varying environment. A diverse suite of behavioral solutions exist for overcoming such environmental variability, including the adjustment of signaling behavior based upon receiver feedback. Few studies have directly examined the relationship between such tactical signaling adjustments and proxies of male fitness; the few that have, failed to find a relationship. Using the wolf spider, Schizocosa rovneri, we set out to first quantify among-male variation in the form and degree of responsiveness to female feedback. Following …

Preventive Effect Of Non-Mitogenic Acidic Fibroblast Growth Factor On Diabetes-Induced Testicular Cell Death., Melissa Skibba 1990-

Electronic Theses and Dissertations

Fibroblast growth factor (FGF)-1 was found to protect the heart from oxidative damage, but clinically its long-term use was restricted for its undesirable proliferating activity on cells. Thus a cluster of amino acids responsible for the proliferation were deleted in the native FGF-1 to create a non-mitogenic FGF-1 (nmFGF-1). To assess whether nmFGF-1 protects male germ cells from diabetes-induced apoptotic death, the study employed a murine model of diabetes using multiple low-doses of streptozotocin, followed by nmFGF-1 treatment for 6 months. Diabetic mice showed a decrease in testicular weight and an increase in apoptotic cell death. Treatment with nmFGF-1 alleviated …

Tertiary First Step To Success : A Process Evaluation Of The Home Component., Charlos Thompson

Electronic Theses and Dissertations

Children with severe behaviors have prompted the response of educators and family interventionists to develop innovative behavioral solutions to address this growing concern. Within the last few years, collaborative behavioral interventions have emerged demonstrating promising solutions for classroom teachers and concerned families. As part of a 3-year study, a U.S. Department of Education (Institute for Education Sciences) development grant was awarded to the Kent School of Social Work. Researchers from the University of Louisville and Oregon Research Institute developed a version of the First Step to Success program for children requiring tertiary-level support. This dissertation examines fidelity levels, social validity, …

Regulation Of Macrophage Inflammatory Signaling Pathways By Amp-Activated Protein Kinase., Yanfang Zhu

Electronic Theses and Dissertations

AMP-activated protein kinase, AMPK, is a conserved serine/threonine kinase with a critical function in the regulation of metabolic pathways in eukaryotic cells. Recently, AMPK has been shown to play an additional role as a regulator of inflammatory activity in leukocytes. Treatment of macrophages with chemical AMPK activators, or forced expression of a constitutively active form of AMPK, results in polarization to an antiinflammatory phenotype. Additionally, we reported previously that stimulation of macrophages with antiinflammatory cytokines such as IL-10, IL-4 and TGF-ß results in rapid activation of AMPK, suggesting that AMPK contributes to the suppressive function of these cytokines. In the …

Advocacy Begins At Home: Methods Of Promoting Faculty Awareness Of Open Access Publishing At The Brooklyn Campus Of Long Island University, Edward Keene

Urban Library Journal

The paper reports on efforts of the Periodicals Librarian at Long Island University’s Brooklyn Campus, a mid-sized urban institution, to measure the awareness of open access (OA) publishing among faculty and document methods that proved effective in raising awareness of the advent of, and debate over, OA publishing. The author reports his survey findings; interprets their implications; reports on the means available at LIU to promote OA and those that proved worthwhile; notes the departmental and committee relationships integral to the process; proposes ideas for future initiatives at LIU/Brooklyn. The findings in the paper will add to the body of …

Coal Ash And Children's Sleep : A Community-Based Study., Clara G. Sears

Electronic Theses and Dissertations

Kentucky is the fifth largest producer of coal ash, a by-product of coal combustion. The small spherical coal ash particles contain heavy metals like arsenic, lead, mercury, and cadmium. Coal ash is currently classified as nonhazardous by the EPA, which allows it to be stored in open-air impoundments near low-income communities. The primary object of the study is to determine the prevalence of sleep disruptive behaviors in children exposed to coal ash, compared to a group of demographically similar non-exposed children. Parents or guardians from five neighborhoods surrounding a coal ash storage facility, and one non-exposed community, participated in a …

Gravitational Wave Astrophysics With Compact Binary Systems, Eric Addison

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Gravitational waves are ripples in the fabric of spacetime that convey information about changing gravitational fields. Large-scale detection projects are currently in operation, and more advanced detectors are being designed and built. Though we have yet to make a direct detection of a gravitational wave signal, upgrades to current detectors are expected to bring the first detections within the next year or two.

Gravitational waves will bring us information about astrophysical phenomena that is complementary to the information gained from photon-based observations (e.g., telescopes and radio receivers). One of the primary sources of gravitational waves are binary systems: two massive …

Movement And Habitat Use Of Bonneville Cutthroat Trout (Oncorhynchus Clarki Utah): A Case Study In The Temple Fork Watershed, Ryan L. Lokteff

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Movement patterns and habitat use of Bonneville cutthroat trout (Oncorhynchus clarki Utah) in tributaries of the Logan River watershed are greatly affected by habitat alterations created by North American Beaver (Castor canadensis). Evaluation of cutthroat trout habitat use in these watersheds is also complicated by biotic interactions with invasive brown trout (Salmo trutta) and brook trout (Salvelinus fontinalis). My objectives in this thesis were to 1.) Evaluate the passage of beaver dams by each trout species in the Temple Fork watershed and 2.) Evaluate the habitat use of cutthroat trout in the presence …

Global Evaluation Of Biofuel Potential From Microalgae, Jeffrey W. Moody

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The objective of the proposed work is to determine the productivity potential of microalgae around the world based on the current large uncertainty of the productivity
potential found in literature. To achieve this objective, a validated thermal and biological growth model was utilized coupled with weather data files from weather stations around the world. This enabled a realistic assessment of the productivity potential based on actual climatic variables. Sensitivity of microalgae lipid productivity to biomass production, temperature, and variability was performed illustrating the importance of biological and temporal resolution. Results from modeling work were leveraged for a scalability assessment based …

A Comparison Of Antlions, Bees, Darkling Beetles And Velvet Ants Across Sand Dune And Non-Sand Dune Habitats At Ash Meadows National Wildlife Refuge, Nicole F. Boehme

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ash Meadows National Wildlife Refuge (AMNWR) supports nearly 30 organisms found nowhere else in the world. Most of these species are aquatic, dwelling in more than 50 seeps and springs within the over 24,000 acres of the refuge. Much of the research at AMNWR has centered on these unique and abundant springfed pools across the desert landscape. Many of the terrestrial habitats and species at the refuge have not been as well studied. In 2008, Bio-West, Inc. in Logan, Utah was contracted to inventory the terrestrial organisms present on the refuge. To investigate the terrestrial invertebrate fauna, with special interest …

Engaging Alternative High School Students Through The Design, Development, And Crafting Of Computationally Enhanced Pets, Maneksha Katrine Dumont

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A new kind of technology combines making in the physical world with computing in the virtual world. These technologies are simultaneously physical and virtual, require the design of artifacts like computer programs and the crafting of physical components. Combining approaches encourage youth to participate in computing who might not otherwise be interested by harnessing existing hobbies and interests. Because fluency with computing is important to success in a 21st century society, increasing the ways in which young people can experience and connect to computing is important. Computational thinking, and with it the process of debugging computer code, is an example …